BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6535

Title: 'Backbone 1H and 15N Chemical Shift Assignments for T antigen origin binding domain in the presence of RPA32C'   PubMed: 15793585

Authors: Arunkumar, Alphonse; Chazin, Walter

Citation: Arunkumar, Alphonse; Klimovich, Vitaly; Jiang, Xiaohua; Ott, Robert; Mizoue, Laura; Fanning, Ellen; Chazin, Walter. "Insights into hRPA32 C-terminal domain-mediated assembly of the simian virus 40 replisome."  Nat. Struct. Mol. Biol. 12, 332-339 (2005).

Assembly members:
Tag-OBD, polymer, 131 residues, Formula weight is not available
RPA32 C-terminal domain, polymer, 103 residues, Formula weight is not available

Natural source:   Common Name: Simian viurs40   Taxonomy ID: not available   Superkingdom: Viruses   Kingdom: not available   Genus/species: Simian viurs40 Simian virus40

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Tag-OBD: GSKVEDPKDFPSELLSFLSH AVFSNRTLACFAIYTTKEKA ALLYKKIMEKYSVTFISRHN SYNHNILFFLTPHRHRVSAI NNYAQKLCTFSFLICKGVNK EYLMYSALTRDPFSVIEESL PGGLKEHDFNP
RPA32 C-terminal domain: ANGLTVAQNQVLNLIKACPR PEGLNFQDLKNQLKHMSVSS IKQAVDFLSNEGHIYSTVDD DHFKSTDAE

Data sets:
Data typeCount
15N chemical shifts165
1H chemical shifts165

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Tag-OBD1
2RPA32C2

Entities:

Entity 1, Tag-OBD 131 residues - Formula weight is not available

1   GLYSERLYSVALGLUASPPROLYSASPPHE
2   PROSERGLULEULEUSERPHELEUSERHIS
3   ALAVALPHESERASNARGTHRLEUALACYS
4   PHEALAILETYRTHRTHRLYSGLULYSALA
5   ALALEULEUTYRLYSLYSILEMETGLULYS
6   TYRSERVALTHRPHEILESERARGHISASN
7   SERTYRASNHISASNILELEUPHEPHELEU
8   THRPROHISARGHISARGVALSERALAILE
9   ASNASNTYRALAGLNLYSLEUCYSTHRPHE
10   SERPHELEUILECYSLYSGLYVALASNLYS
11   GLUTYRLEUMETTYRSERALALEUTHRARG
12   ASPPROPHESERVALILEGLUGLUSERLEU
13   PROGLYGLYLEULYSGLUHISASPPHEASN
14   PRO

Entity 2, RPA32C 103 residues - Formula weight is not available

1   ALAASNGLYLEUTHRVALALAGLNASNGLN
2   VALLEUASNLEUILELYSALACYSPROARG
3   PROGLUGLYLEUASNPHEGLNASPLEULYS
4   ASNGLNLEULYSHISMETSERVALSERSER
5   ILELYSGLNALAVALASPPHELEUSERASN
6   GLUGLYHISILETYRSERTHRVALASPASP
7   ASPHISPHELYSSERTHRASPALAGLU

Samples:

sample_1: Tag-OBD, [U-15N], 0.2 mM; RPA32 C-terminal domain, unlabeled, 0.6 mM; Tris, 2H, 20 mM; MgCl2 5 mM; DTT 2 mM

sample_2: Tag-OBD, unlabeled, 0.2 mM; RPA32 C-terminal domain, [U-15N], 0.6 mM; Tris, 2H, 20 mM; MgCl2 5 mM; DTT 2 mM

conditions_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQCnot availableisotropicconditions_1

Software:

FELIX, Accelrys Inc -

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 17060 4460
PDB
DBJ BAG35795 BAG59456 BAG60901 BAG62374 BAI47325
EMBL CAG29344 CAH90667
GB AAA36560 AAH01630 AAH12157 AAH21257 AAX84514
REF NP_001125362 NP_001231871 NP_001247563 NP_001273005 NP_001284487
SP P15927 Q5RC43