BMRB Entry 17060

Name: Replication protein A 32 interacts through a similar binding interface with TIPIN, XPA, and UNG2
Published: 2010-09-08

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR17060

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Replication protein A 32 interacts through a similar binding interface with TIPIN, XPA, and UNG2

Deposition date:

2010-07-13

Original release date:

2010-09-08

Authors:

Ali, Seikh; Shin, Jae-Sun; Bae, Sung-Hun; Kim, Byoungkook; Choi, Byong-Seok

Citation:

Citation: Ali, Seikh; Shin, Jae-Sun; Bae, Sung-Hun; Kim, Byoungkook; Choi, Byong-Seok. "Replication protein A 32 interacts through a similar binding interface with TIPIN, XPA, and UNG2" INT. J. BIOCHEM. CELL B 42, 1210-1215 (2010).

Assembly members:

Assembly members:
RPA32C, polymer, 97 residues, Formula weight is not available
TIPIN, polymer, 34 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RPA32C: SQPSAGRAPISNPGMSEAGN FGGNSFMPANGLTVAQNQVL NLIKACPRPEGLNFQDLKNQ LKHMSVSSIKQAVDFLSNEG HIYSTVDDDHFKSTDAE
TIPIN: EMFASELSRSLTEEQQRRIE RNKQLALERRQAKL

Data sets:

binding_constants

Data type	Count
binding constants	1

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RPA32C	1
2	TIPIN	2

Entities:

Entity 1, RPA32C 97 residues - Formula weight is not available

1

SER

GLN

PRO

SER

ALA

GLY

ARG

ALA

PRO

ILE

2

SER

ASN

PRO

GLY

MET

SER

GLU

ALA

GLY

ASN

3

PHE

GLY

ASN

SER

PHE

MET

PRO

ALA

ASN

4

GLY

LEU

THR

VAL

ALA

GLN

ASN

GLN

VAL

LEU

5

ASN

LEU

ILE

LYS

ALA

CYS

PRO

ARG

PRO

GLU

6

GLY

LEU

ASN

PHE

GLN

ASP

LEU

LYS

ASN

GLN

7

LEU

LYS

HIS

MET

SER

VAL

SER

ILE

LYS

8

GLN

ALA

VAL

ASP

PHE

LEU

SER

ASN

GLU

GLY

9

HIS

ILE

TYR

SER

THR

VAL

ASP

HIS

10

PHE

LYS

SER

THR

ASP

ALA

GLU

Entity 2, TIPIN 34 residues - Formula weight is not available

1

GLU

MET

PHE

ALA

SER

GLU

LEU

SER

ARG

SER

2

LEU

THR

GLU

GLN

ARG

ILE

GLU

3

ARG

ASN

LYS

GLN

LEU

ALA

LEU

GLU

ARG

4

GLN

ALA

LYS

LEU

Samples:

sample_1: sodium phosphate 25 mM; sodium chloride 50 mM; DTT 3 mM; RPA32C, [U-15N], 0.1 mM; TIPIN0.02 – 3.0 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian not given 600 MHz
Bruker not given 800 MHz

BMRB	4460
PDB	1DPU 1Z1D 2PI2 2Z6K 4MQV 4OU0
DBJ	BAG35795 BAG59456 BAG60901 BAG62374 BAI47325 BAA91229 BAG61265 BAI46472
EMBL	CAG29344 CAH90667 CAL38286
GB	AAA36560 AAH01630 AAH12157 AAH21257 AAX84514 AAH00870 ACB21044 AIC51688 EAW77762
REF	NP_001125362 NP_001247563 NP_001273005 NP_001284487 NP_002937 NP_001276915 NP_060328 XP_005254578 XP_005254579 XP_006720656
SP	P15927 Q5RC43 Q9BVW5
TPG	DAA01365
AlphaFold	P15927 Q5RC43 Q9BVW5