BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5931

Title: Dynamics of the RNase H Domain of HIV-1 Reverse Transcriptase in the Presence of Magnesium and AMP   PubMed: 12534276

Deposition date: 2003-09-03 Original release date: 2004-04-07

Authors: Pari, Koteppa; Mueller, Geoffrey; DeRose, Eugene; Kirby, Thomas; London, Robert

Citation: Pari, Koteppa; Mueller, Geoffrey; DeRose, Eugene; Kirby, Thomas; London, Robert. "Solution structure of the RNase H domain of the HIV-1 reverse transciptase in the presence of magnesium."  Biochemistry 42, 639-650 (2003).

Assembly members:
HIV-1 RNase H, polymer, 138 residues, 15243 Da.
MG, non-polymer, 24.305 Da.
AMP, non-polymer, 347.221 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: viruses   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
HIV-1 RNase H: MNELYQLEKEPIVGAETFYV DGAANRETKLGKAGYVTNRG RQKVVTLTDTTNQKTELQAI YLALQDSGLEVNIVTDSQYA LGIIQAQPDQSESELVNQII EQLIKKEKVYLAWVPAHKGI GGNEQVDKLVSAGIRKVL

Data sets:
Data typeCount
1H chemical shifts980
13C chemical shifts587
15N chemical shifts150

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HIV-1 RT, RNase H domain1
2Mg1
3AMP1

Entities:

Entity 1, HIV-1 RT, RNase H domain 138 residues - 15243 Da.

1   METASNGLULEUTYRGLNLEUGLULYSGLU
2   PROILEVALGLYALAGLUTHRPHETYRVAL
3   ASPGLYALAALAASNARGGLUTHRLYSLEU
4   GLYLYSALAGLYTYRVALTHRASNARGGLY
5   ARGGLNLYSVALVALTHRLEUTHRASPTHR
6   THRASNGLNLYSTHRGLULEUGLNALAILE
7   TYRLEUALALEUGLNASPSERGLYLEUGLU
8   VALASNILEVALTHRASPSERGLNTYRALA
9   LEUGLYILEILEGLNALAGLNPROASPGLN
10   SERGLUSERGLULEUVALASNGLNILEILE
11   GLUGLNLEUILELYSLYSGLULYSVALTYR
12   LEUALATRPVALPROALAHISLYSGLYILE
13   GLYGLYASNGLUGLNVALASPLYSLEUVAL
14   SERALAGLYILEARGLYSVALLEU

Samples:

sample_1: HIV-1 RNase H 1.1 mM

Exp-cond_1: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availableExp-cond_1
1H-13C HSQCsample_1not availableExp-cond_1
HNCACBsample_1not availableExp-cond_1
CBCA(CO)NHsample_1not availableExp-cond_1
HNCOsample_1not availableExp-cond_1
H(CCO)NHsample_1not availableExp-cond_1
(H)C(CO)NH TOCSYsample_1not availableExp-cond_1
(HB)CB(CGCD)HDsample_1not availableExp-cond_1
(HB)CB(CGCDCE)HEsample_1not availableExp-cond_1
CN-NOESY HSQCsample_1not availableExp-cond_1

Software:

NMRPipe -

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 4093 5347 5932
PDB
DBJ BAA12997 BAH96515 BAH96524
GB AAA93161 AAA93162 AAA93165 AAB05599 AAB50259
PIR GNVWLV
REF NP_057849 NP_705927 NP_789740 YP_001856242
SP P03367 P04585

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts