BMRB Entry 4093

Title:
Secondary Structure of the Ribonuclease H Domain of the Human Immunodeficiency Virus Reverse Transcriptase in Solution Using Three-Dimensional Double and Triple Resonance Heteronuclear Magnetic Resonance Spectroscopy
Deposition date:
1998-01-08
Original release date:
1998-03-02
Authors:
Powers, Robert; Clore, G.; Bax, Ad; Garrett, Daniel; Stahl, Stephen; Wingfield, Paul; Gronenborn, Angela
Citation:

Citation: Powers, Robert; Clore, G.; Bax, Ad; Garrett, Daniel; Stahl, Stephen; Wingfield, Paul; Gronenborn, Angela. "Secondary Structure of the Ribonuclease H Domain of the Human Immunodeficiency Virus Reverse Transcriptase in Solution Using Three-Dimensional Double and Triple Resonance Heteronuclear Magnetic Resonance Spectroscopy"  J. Biomol. NMR 221, 1081-1090 (1991).

Assembly members:

Assembly members:
Reverse Transcriptase of HIV-1, polymer, 138 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: HIV-1   Taxonomy ID: 11706   Superkingdom: not available   Kingdom: not available   Genus/species: Lentivirus HIV-1

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts239
15N chemical shifts117
1H chemical shifts243

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNase_H-HIV-11

Entities:

Entity 1, RNase_H-HIV-1 138 residues - Formula weight is not available

1   METASNGLULEUTYRGLNLEUGLULYSGLU
2   PROILEVALGLYALAGLUTHRPHETYRVAL
3   ASPGLYALAALAASNARGGLUTHRLYSLEU
4   GLYLYSALAGLYTYRVALTHRASNARGGLY
5   ARGGLNLYSVALVALTHRLEUTHRASPTHR
6   THRASNGLNLYSTHRGLULEUGLNALAILE
7   TYRLEUALALEUGLNASPSERGLYLEUGLU
8   VALASNILEVALTHRASPSERGLNTYRALA
9   LEUGLYILEILEGLNALAGLNPROASPGLN
10   SERGLUSERGLULEUVALASNGLNILEILE
11   GLUGLNLEUILELYSLYSGLULYSVALTYR
12   LEUALAALAVALPROALAHISLYSGLYILE
13   GLYGLYASNGLUGLNVALASPLYSLEUVAL
14   SERALAGLYILEARGLYSVALLEU

Samples:

sample_one: Reverse Transcriptase of HIV-1, [U-95% 13C; U-95% 15N], 1.1 mM; H2O 90%; D2O 10%; Sodium phosphate buffer 100 mM

sample_conditions: pH: 5.4; temperature: 299 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker AM600 600 MHz

Related Database Links:

PDB
GB AAA93161 AAB50259 AAC55949 AAC55950 AAC82598
REF NP_057849 NP_705927 NP_789740 YP_001856242
SP P04585
AlphaFold P04585

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks