BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5347

Title: Solution Structure of the RNase H Domain of the HIV-1 Reverse Transcriptase in Presence of Magnesium   PubMed: 12534276

Authors: Pari, Koteppa; Mueller, Geoffrey; DeRose, Eugene; Kirby, Thomas; London, Robert

Citation: Pari, Koteppa; Mueller, Geoffrey; DeRose, Eugene; Kirby, Thomas; London, Robert. "Solution Structure of the RNase H Domain of the HIV-1 Reverse Transcriptase in the Presence of Magnesium"  Biochemistry 42, 639-650 (2003).

Assembly members:
HIV-1 RNase H, polymer, 138 residues, 15243 Da.
MG, non-polymer, 24.305 Da.

Natural source:   Common Name: Human immunodeficiency virus type 1 (HXB2 isolate)   Taxonomy ID: 11706   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lentivirus Human immunodeficincy virus type 1

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
HIV-1 RNase H: MNELYQLEKEPIVGAETFYV DGAANRETKLGKAGYVTNRG RQKVVTLTDTTNQKTELQAI YLALQDSGLEVNIVTDSQYA LGIIQAQPDQSESELVNQII EQLIKKEKVYLAWVPAHKGI GGNEQVDKLVSAGIRKVL

Data sets:
Data typeCount
1H chemical shifts912
13C chemical shifts552
15N chemical shifts139

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HIV-1 RT1
2Magnesium ion2

Entities:

Entity 1, HIV-1 RT 138 residues - 15243 Da.

1   METASNGLULEUTYRGLNLEUGLULYSGLU
2   PROILEVALGLYALAGLUTHRPHETYRVAL
3   ASPGLYALAALAASNARGGLUTHRLYSLEU
4   GLYLYSALAGLYTYRVALTHRASNARGGLY
5   ARGGLNLYSVALVALTHRLEUTHRASPTHR
6   THRASNGLNLYSTHRGLULEUGLNALAILE
7   TYRLEUALALEUGLNASPSERGLYLEUGLU
8   VALASNILEVALTHRASPSERGLNTYRALA
9   LEUGLYILEILEGLNALAGLNPROASPGLN
10   SERGLUSERGLULEUVALASNGLNILEILE
11   GLUGLNLEUILELYSLYSGLULYSVALTYR
12   LEUALATRPVALPROALAHISLYSGLYILE
13   GLYGLYASNGLUGLNVALASPLYSLEUVAL
14   SERALAGLYILEARGLYSVALLEU

Entity 2, Magnesium ion - Mg - 24.305 Da.

1   MG

Samples:

Sample_1: HIV-1 RNase H, [U-90% 13C; U-90% 15N], 1.1 mM; magnesium 80 mM

Experimental_Conditions: pH: 6.8; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCSample_1not availableExperimental_Conditions
1H-13C HSQCSample_1not availableExperimental_Conditions
HNCACBSample_1not availableExperimental_Conditions
CBCA(CO)NHSample_1not availableExperimental_Conditions
HNCOSample_1not availableExperimental_Conditions
H(CCO)NHSample_1not availableExperimental_Conditions
(H)C(CO)NH TOCSYSample_1not availableExperimental_Conditions
(HB)CB(CGCD)HDSample_1not availableExperimental_Conditions
(HB)CB(CGCDCE)HESample_1not availableExperimental_Conditions
CN-NOESY HSQCSample_1not availableExperimental_Conditions

Software:

NMRPipe - spectra processing

NMRView - spectra processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

BMRB 4093 5931 5932
PDB
DBJ BAA12997 BAH96515 BAH96524
GB AAA93161 AAA93162 AAA93165 AAB05599 AAB50259
PIR GNVWLV
REF NP_057849 NP_705927 NP_789740 YP_001856242
SP P03367 P04585