BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 5306

Title: 1H, 13C, and 15N Chemical Shift Assignments for the HIF-1alpha CTAD/p300 CH1 Complex   PubMed: 11959990

Authors: Freedman, Steven; Sun, Zhen-Yu; Poy, Florence; Kung, Andrew; Livingston, David; Wagner, Gerhard; Eck, Michael

Citation: Freedman, Steven; Sun, Zhen-Yu; Poy, Florence; Kung, Andrew; Livingston, David; Wagner, Gerhard; Eck, Michael. "Structural Basis for Recruitment of CBP/p300 by Hypoxia-inducible Factor-1 alpha "  Proc. Natl. Acad. Sci. U. S. A. 99, 5367-5372 (2002).

Assembly members:
C-terminal activation domain of hypoxia inducible factor-1alpha, polymer, 42 residues, Formula weight is not available
p300 cysteine/histidine-rich 1 domain, polymer, 101 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
C-terminal activation domain of hypoxia inducible factor-1alpha: GSMDESGLPQLTSYDCEVNA PIQGSRNLLQGEELLRALDQ VN
p300 cysteine/histidine-rich 1 domain: MGSGAHTADPEKRKLIQQQL VLLLHAHKCQRREQANGEVR QCNLPHCRTMKNVLNHMTHC QSGKSCQVAHCASSRQIISH WKNCTRHDCPVCLPLKNAGD K

Data sets:
Data typeCount
1H chemical shifts805
13C chemical shifts557
15N chemical shifts146

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HIF-1alpha C-terminal activation domain1
2p300 cysteine/histidine-rich 1 domain2
3ZINC (II) ION, I3
4ZINC (II) ION, II3
5ZINC (II) ION, III3

Entities:

Entity 1, HIF-1alpha C-terminal activation domain 42 residues - Formula weight is not available

1   GLYSERMETASPGLUSERGLYLEUPROGLN
2   LEUTHRSERTYRASPCYSGLUVALASNALA
3   PROILEGLNGLYSERARGASNLEULEUGLN
4   GLYGLUGLULEULEUARGALALEUASPGLN
5   VALASN

Entity 2, p300 cysteine/histidine-rich 1 domain 101 residues - Formula weight is not available

1   METGLYSERGLYALAHISTHRALAASPPRO
2   GLULYSARGLYSLEUILEGLNGLNGLNLEU
3   VALLEULEULEUHISALAHISLYSCYSGLN
4   ARGARGGLUGLNALAASNGLYGLUVALARG
5   GLNCYSASNLEUPROHISCYSARGTHRMET
6   LYSASNVALLEUASNHISMETTHRHISCYS
7   GLNSERGLYLYSSERCYSGLNVALALAHIS
8   CYSALASERSERARGGLNILEILESERHIS
9   TRPLYSASNCYSTHRARGHISASPCYSPRO
10   VALCYSLEUPROLEULYSASNALAGLYASP
11   LYS

Entity 3, ZINC (II) ION, I - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: C-terminal activation domain of hypoxia inducible factor-1alpha 1.0 mM; p300 cysteine/histidine-rich 1 domain 1.0 mM; ZnSO4 0.1 mM; DTT 1 mM; NaCl 100 mM

sample_2: C-terminal activation domain of hypoxia inducible factor-1alpha, [U-15N], 1.0 mM; p300 cysteine/histidine-rich 1 domain, [U-15N], 1.0 mM; ZnSO4 0.1 mM; DTT 1 mM; NaCl 100 mM

sample_3: C-terminal activation domain of hypoxia inducible factor-1alpha, [U-13C; U-15N], 1.0 mM; p300 cysteine/histidine-rich 1 domain, [U-13C; U-15N], 1.0 mM; ZnSO4 0.1 mM; DTT 1 mM; NaCl 100 mM

Condition_1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availableCondition_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Varian UnityInova 750 MHz
  • Varian Unity 500 MHz

Related Database Links:

BMRB 5788 5788
PDB
DBJ BAC31311 BAG10860 BAG10860 BAC31311
EMBL CAF93378 CAF95299 CAG04516 CBN81203 CDQ80651 CAH73688 CAH70384 CAG04516 CAF95299 CAF93378
GB AAA18639 AAB31182 AAH53889 AAI44977 AAI50682
REF NP_001231599 NP_001253415 NP_001420 NP_808489 XP_001076610 XP_001168324 XP_001102844 XP_001076610 NP_808489 NP_001420
SP B2RWS6 Q09472
SWISS-PROT Q09472
GenBank EAW60408 AAI50682 AAH53889 AAB31182 AAA18639