BMRB Entry 5788

Title:
Structural basis for negative regulation of hypoxia-inducible factor-1alpha by CITED2
Deposition date:
2003-04-29
Original release date:
2003-08-01
Authors:
Freedman, Steven; Sun, Zhen-Yu; Kung, Andrew; France, Dennis; Wagner, Gerhard; Eck, Michael
Citation:

Citation: Freedman, Steven; Sun, Zhen-Yu; Kung, Andrew; France, Dennis; Wagner, Gerhard; Eck, Michael. "Structural Basis for Negative Regulation of Hypoxia-inducible Factor-1alpha by CITED2"  Nat. Struct. Biol. 10, 504-512 (2003).
PubMed: 12778114

Assembly members:

Assembly members:
CITED2 transactivation domain, polymer, 52 residues, Formula weight is not available
p300 cysteine/histidine-rich 1 domain, polymer, 101 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts855
13C chemical shifts546
15N chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CITED21
2p300 cysteine histidine-rich 1 domain2

Entities:

Entity 1, CITED2 52 residues - Formula weight is not available

1   GLYSERGLYSERGLYSERGLYSERASNVAL
2   ILEASPTHRASPPHEILEASPGLUGLUVAL
3   LEUMETSERLEUVALILEGLUMETGLYLEU
4   ASPARGILELYSGLULEUPROGLULEUTRP
5   LEUGLYGLNASNGLUPHEASPPHEMETTHR
6   ASPPHE

Entity 2, p300 cysteine histidine-rich 1 domain 101 residues - Formula weight is not available

1   METGLYSERGLYALAHISTHRALAASPPRO
2   GLULYSARGLYSLEUILEGLNGLNGLNLEU
3   VALLEULEULEUHISALAHISLYSCYSGLN
4   ARGARGGLUGLNALAASNGLYGLUVALARG
5   GLNCYSASNLEUPROHISCYSARGTHRMET
6   LYSASNVALLEUASNHISMETTHRHISCYS
7   GLNSERGLYLYSSERCYSGLNVALALAHIS
8   CYSALASERSERARGGLNILEILESERHIS
9   TRPLYSASNCYSTHRARGHISASPCYSPRO
10   VALCYSLEUPROLEULYSASNALAGLYASP
11   LYS

Samples:

sample_1: CITED2 transactivation domain, [U-15N; U-13C], 1.0 mM; p300_CH1, [U-15N; U-13C], 1.0 mM; deut-MES 10 mM; NaCl 100 mM; ZnSO4 0.1 mM; deut-DTT 1 mM

sample_2: CITED2 transactivation domain, [U-15N], 1.0 mM; p300 cysteine/histidine-rich 1 domain, [U-15N], 1.0 mM; deut-MES 10 mM; NaCl 100 mM; ZnSO4 0.1 mM; deut-DTT 1 mM

sample_3: CITED2 transactivation domain 1.0 mM; p300 cysteine/histidine-rich 1 domain 1.0 mM; deut-MES 10 mM; NaCl 100 mM; ZnSO4 0.1 mM; deut-DTT 1 mM

cond_1: pH: 6.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
CCONHnot availablenot availablenot available
HCCONHnot availablenot availablenot available
1H-15N-TOCSYnot availablenot availablenot available
1H-13C-HCCH-TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available
1H-1H 2D NOESYnot availablenot availablenot available

Software:

PROSA - NMR processing

XEASY - peak analysis

NMR spectrometers:

  • Varian UnityInova 500 MHz
  • Varian UnityInova 750 MHz

Related Database Links:

BMRB 5306 5306
PDB
DBJ BAC31311 BAG10860 BAG10860 BAC31311
EMBL CAF93378 CAF95299 CAG04516 CBN81203 CDQ80651 CAH73688 CAH70384 CAG04516 CAF95299 CAF93378
GB AAA18639 AAB31182 AAH53889 AAI44977 AAI50682
REF NP_001231599 NP_001253415 NP_001420 NP_808489 XP_001076610 XP_001168324 XP_001102844 XP_001076610 NP_808489 NP_001420
SP B2RWS6 Q09472
SWISS-PROT Q09472
GenBank EAW60408 AAI50682 AAH53889 AAB31182 AAA18639
AlphaFold B2RWS6 Q09472

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks