BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 15245

Title: NMR SOLUTION STRUCTURE OF THE VILLIN HEADPIECE MUTANT G34L   PubMed: 18284690

Authors: Gronwald, Wolfram; Hohm, Tim; Hoffmann, Daniel

Citation: Gronwald, Wolfram; Hohm, Tim; Hoffmann, Daniel. "Evolutionary Pareto-optimization of stably folding peptides"  BMC Bioinformatics. 9, .-. (2008).

Assembly members:
villin_G34L, polymer, 36 residues, Formula weight is not available

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: chemical synthesis   Host organism: none

Entity Sequences (FASTA):
villin_G34L: MLSDEDFKAVFGMTRSAFAN LPLWKQQNLKKEKLLF

Data sets:
Data typeCount
1H chemical shifts219

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1villin_G34L monomer1

Entities:

Entity 1, villin_G34L monomer 36 residues - Formula weight is not available

1   METLEUSERASPGLUASPPHELYSALAVAL
2   PHEGLYMETTHRARGSERALAPHEALAASN
3   LEUPROLEUTRPLYSGLNGLNASNLEULYS
4   LYSGLULYSLEULEUPHE

Samples:

sample_1: villin_G34L 4.3 mg; H2O 90 v/v %; D2O 10 v/v %; DSS 0.1 mM

sample_conditions_1: ionic strength: 0 M; pH: 5.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

AUREMOL v1.3, Bruker Biospin - chemical shift assignment, data analysis, peak picking

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

BMRB 11519 11520 11521 15097 17698 4428
PDB
GB AAA49133
REF NP_990773 XP_003207697 XP_010711916 XP_010711917
SP P02640