BMRB Entry 11521

Title:
The chemical shift assignments of the headpiece subdomain of chicken villin protein under the crowded condition with the segment B1 of streptococcal protein G
Deposition date:
2012-12-13
Original release date:
2013-02-15
Authors:
Tochio, Naoya; Kigawa, Takanori
Citation:

Citation: Harada, Ryuhei; Tochio, Naoya; Kigawa, Takanori; Sugita, Yuji; Feig, Michael. "Reduced native state stability in crowded cellular environment due to protein-protein interactions"  .

Assembly members:

Assembly members:
protein, polymer, 36 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: E. coli - cell free   Vector: P120522-02

Entity Sequences (FASTA):

Entity Sequences (FASTA):
protein: MLSDEDFKAVFGMTRSAFAN LPLWKQQNLKKEKGLF

Data sets:
Data typeCount
15N chemical shifts33
1H chemical shifts33

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Villin1

Entities:

Entity 1, Villin 36 residues - Formula weight is not available

1   METLEUSERASPGLUASPPHELYSALAVAL
2   PHEGLYMETTHRARGSERALAPHEALAASN
3   LEUPROLEUTRPLYSGLNGLNASNLEULYS
4   LYSGLULYSGLYLEUPHE

Samples:

sample_1: protein, [U-15N], 1 mM; HEPES 50 mM; protein G 16 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Kujira, N. Kobayashi - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 11519 11520 15097 15245 17698 19911 4428
PDB
GB AAA49133
REF NP_990773 XP_003207697 XP_010711916 XP_010711917
SP P02640
AlphaFold P02640

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks