BMRB Entry 16996

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for human Rad51D from 1 to 83
Deposition date:
2010-06-11
Original release date:
2011-06-01
Authors:
Choi, Byong-Seok; Kim, Young Mee
Citation:

Citation: Kim, Young Mee; Choi, Byong-Seok. "Structural and functional characterization of the N-terminal domain of human Rad51D."  Int. J. Biochem. Cell Biol. 43, 416-422 (2011).
PubMed: 21111057

Assembly members:

Assembly members:
human_Rad51D_N, polymer, 83 residues, 9070.740 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-28a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts333
15N chemical shifts80
1H chemical shifts587

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human Rad51D N1

Entities:

Entity 1, human Rad51D N 83 residues - 9070.740 Da.

1   METGLYVALLEUARGVALGLYLEUCYSPRO
2   GLYLEUTHRGLUGLUMETILEGLNLEULEU
3   ARGSERHISARGILELYSTHRVALVALASP
4   LEUVALSERALAASPLEUGLUGLUVALALA
5   GLNLYSCYSGLYLEUSERTYRLYSALALEU
6   VALALALEUARGARGVALLEULEUALAGLN
7   PHESERALAPHEPROVALASNGLYALAASP
8   LEUTYRGLUGLULEULYSTHRSERTHRALA
9   ILELEUSER

Samples:

sample_1: human Rad51D_N, [U-100% 15N], 1 mM; DTT 1 mM; sodium azide 0.02%; Tris-HCl 25 mM; NaCl 100 mM; D2O 100%

sample_2: human Rad51D_N, [U-99% 13C; U-99% 15N], 1 mM; TRIS 25 mM; sodium chloride 100 mM; DTT 1 mM; sodium azide 0.02%; D2O 100%

sample_3: human Rad51D_N, [U-99% 15N], 1 mM; TRIS 25 mM; sodium chloride 100 mM; DTT 1 mM; sodium azide 0.02%; D2O 90%; H2O 10%

sample_conditions_1: ionic strength: 100 mM; pH: 7.5; pressure: 1 atm; temperature: 287 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D 1H-15N TOCSYsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, P.GUNTERT ET AL. - refinement

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Bruker Avance 800 MHz

Related Database Links:

PDB
DBJ BAA25914 BAA31747 BAA31748 BAA33779 BAA33780
EMBL CAA75681
GB AAC39719 AAH14422 AAT38112 ABM83244 ABM86446
REF NP_002869 XP_001174459 XP_003818041 XP_004041973 XP_008969520
SP O75771
AlphaFold O75771

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks