BMRB Entry 19959

Title:
Structural basis for binding of Pan3 to Pan2 and its function in mRNA recruitment and deadenylation
Deposition date:
2014-05-08
Original release date:
2014-05-20
Authors:
Wolf, J.; Valkov, E.; Allen, Mark; Meineke, B.; Gordiyenko, Y.; Mclaughlin, S.; Olsen, T.; Robinson, C.; Bycroft, M.; Stewart, M.; Passmore, L.; Olsen, S.
Citation:

Citation: Wolf, J.; Valkov, E.; Allen, Mark; Meineke, B.; Gordiyenko, Y.; Mclaughlin, S.; Olsen, T.; Robinson, C.; Bycroft, M.; Stewart, M.; Passmore, L.. "Structural basis for binding of Pan3 to Pan2 and its function in mRNA recruitment and deadenylation"  To Be Published ., .-..

Assembly members:

Assembly members:
PAB-DEPENDENT_POLY(A)-SPECIFIC_RIBONUCLEASE_SUBUN, polymer, 41 residues, 4715.2286 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI B41   Vector: pHLTV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PAB-DEPENDENT_POLY(A)-SPECIFIC_RIBONUCLEASE_SUBUN: MDKINPDWAKDIPCRNITIY GYCKKEKEGCPFKHSDNTTA T

Data sets:
Data typeCount
1H chemical shifts285
13C chemical shifts121
15N chemical shifts36

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PAB-DEPENDENT POLY(A)-SPECIFIC RIBONUCLEASE SUBUN1
2ZINC ION2

Entities:

Entity 1, PAB-DEPENDENT POLY(A)-SPECIFIC RIBONUCLEASE SUBUN 41 residues - 4715.2286 Da.

1   METASPLYSILEASNPROASPTRPALALYS
2   ASPILEPROCYSARGASNILETHRILETYR
3   GLYTYRCYSLYSLYSGLULYSGLUGLYCYS
4   PROPHELYSHISSERASPASNTHRTHRALA
5   THR

Entity 2, ZINC ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: PAB-DEPENDENT POLY(A)-SPECIFIC RIBONUCLEASE SUBUN, [U-13C; U-15N], 1.5 mM; Tris 20 mM; NaCl 100 mM

sample_conditions_1: ionic strength: 100.000 mM; pH: 7.000; pressure: 1 atm; temperature: 293.000 K

Experiments:

NameSampleSample stateSample conditions
nasample_1solutionsample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

CNS vany, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker Avance . MHz

Related Database Links:

GB EGA85959.1 AHY76216 AJP40010 AJS30208 AJS30509 AJS30808
UNP PAN3_YEAST
PDB
DBJ GAA24697
EMBL CAA81860 CAY81058
REF NP_012900
SP P36102
TPG DAA09129
AlphaFold D6VXQ9 P36102

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks