BMRB Entry 16653

Title:
1H, 13C and 15N resonance assignment of the PDZ domain of HtrA from Streptococcus pneumonia
Deposition date:
2009-12-23
Original release date:
2010-05-05
Authors:
Fan, Kai; Shang, Qiang; Zhang, Jiahai; Tu, Xiaoming
Citation:

Citation: Fan, Kai; Zhang, Jiahai; Shang, Qiang; Tu, Xiaoming. "1H, 13C and 15N resonance assignment of the PDZ domain of HtrA from Streptococcus pneumoniae."  Biomol. NMR Assignments 4, 79-82 (2010).
PubMed: 20437142

Assembly members:

Assembly members:
HtrA-PDZ, polymer, 134 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Streptococcus pneumoniae   Taxonomy ID: 1313   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus pneumoniae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET22

Data sets:
Data typeCount
13C chemical shifts376
15N chemical shifts119
1H chemical shifts796

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HtrA_PDZ, chain 11
2HtrA_PDZ, chain 21

Entities:

Entity 1, HtrA_PDZ, chain 1 134 residues - Formula weight is not available

1   METGLUGLYLEUGLYPHEALAILEPROALA
2   ASNASPALAILEASNILEILEGLUGLNLEU
3   GLULYSASNGLYLYSVALTHRARGPROALA
4   LEUGLYILEGLNMETVALASNLEUSERASN
5   VALSERTHRSERASPILEARGARGLEUASN
6   ILEPROSERASNVALTHRSERGLYVALILE
7   VALARGSERVALGLNSERASNMETPROALA
8   ASNGLYHISLEUGLULYSTYRASPVALILE
9   THRLYSVALASPASPLYSGLUILEALASER
10   SERTHRASPLEUGLNSERALALEUTYRASN
11   HISSERILEGLYASPTHRILELYSILETHR
12   TYRTYRARGASNGLYLYSGLUGLUTHRTHR
13   SERILELYSLEUASNLYSLEUGLUHISHIS
14   HISHISHISHIS

Samples:

sample_1: HtrA-PDZ, [U-99% 13C; U-99% 15N], 0.6 mM; sodium chloride 100 mM; sodium phosphate 25 mM; DTT 2 mM; EDTA 1.5 mM; D2O 10%

sample_conditions_1: ionic strength: 125 mM; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

PDB
EMBL CAR70001 CBW33633 CBW35682 CBW37622 CCM09372
GB AAC45334 AAK76286 AAL00847 ABJ54994 ACA35689
REF NP_359636 WP_000197799 WP_000681590 WP_000681595 WP_000681597

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks