PDB Newsletter Number 4 -- Winter, 1999 -- RCSB Published quarterly by the Research Collaboratory for Structural Bioinformatics Weekly PDB news is available on the Web at http://www.rcsb.org/pdb/latest_news.html. This newsletter will soon be available on the Web in HTML format at http://www.rcsb.org/pdb/newsletter/ SNAPSHOT -- December 21, 1999 11,317 released atomic coordinate entries Molecule Type 10044 proteins, peptides, and viruses 768 nucleic acids 487 protein/nucleic acid complexes 18 carbohydrates Experimental Technique 9263 diffraction and other 3277 structure factor files 1800 NMR 681 NMR restraint files 254 theoretical modeling TABLE OF CONTENTS Message from the PDB PDB News Citing the PDB Availability of PDB Services December 31, 1999, through January 3, 2000 PDB Newsletter - Final Advisory PDB at the Biophysical Society Annual Meeting New Building for the RU PDB Team One-Year Anniversary of RCSB PDB Grant Award Data Deposition, Processing, and Uniformity Summary Completion of Layer 1 Entry Processing Processing of Backlog Structures Completed PDB Format Changes Implemented Data Distribution and Query Summary CD-ROM of PDB Holdings Now Available PDB Opens Beta Test Site New RCSB PDB Mirrors Redirection of Traffic from the BNL PDB Site Recent Additions to the PDB Web Site New Visualization Applications on the PDB Web Site Outreach Activities Two Workshops on Structural Genomics New Web Site for Synchrotron Users Hand-held Models of Proteins RFP for CORBA Standard for Biological Macromolecular Structure Data Statement of Support RCSB PDB Team ----------------------------------------- MESSAGE FROM THE PDB In the past several months, the PDB has continued to concentrate on data processing, uniformity, query, and distribution. This newsletter summarizes the overall activities in these areas while bulleting several items of particular note. A detailed summary of PDB activities will be made available in the forthcoming PDB Annual Report. We look forward to working with all of you in the coming year, and hope to meet you in person at upcoming meetings and workshops. Comments and questions about the PDB and this newsletter are always welcome at info@rcsb.org. Best wishes for a happy new year. The PDB ----------------------------------------- PDB NEWS *Citing the PDB A journal reference is now available for citing the PDB: H.M.Berman, J.Westbrook, Z.Feng, G.Gilliland, T.N.Bhat, H.Weissig, I.N.Shindyalov, P.E.Bourne The Protein Data Bank Nucleic Acids Research, 28 pp. 235-242 (2000) *Availability of PDB Services December 31, 1999, through January 3, 2000 We have taken all reasonable precautions to ensure that the software and hardware supporting PDB operations are Y2K compliant. While we do not anticipate any problems with our local facilities related to Y2K, we are concerned about the potential for disruption of network communication and the associated adverse affect this could have on the deposition process. In particular, we want to avoid the loss or corruption of any deposited data. As a result, we believe that it is prudent to shutdown PDB deposition operations during this uncertain period. The following summarizes the availability of PDB services from December 31, 1999, through January 3, 1999. WWW query and ftp services: All query, ftp, and e-mail services provided by the PDB will remain on-line during this period. Data Deposition services: We plan to turn off the ADIT deposition server to correspond to the worldwide transition to the year 2000 at 6 am EST December 31, 1999. We will be testing the ADIT system on January 2, 1999, and if all goes well we plan to restore deposition services at 9 am EST January 3, 1999. Although ADIT and AUTODEP at the Rutgers site will not be available during this period, the deposition server system will remain on-line and will accept e-mail during this period. *PDB Newsletter - Final Advisory Based on feedback from the September 1, 1999, Advisory Notice, the PDB plans to discontinue the printed Newsletter starting with this current issue. The Newsletter will continue to be available from the Web site in HTML format and a text version will continue to be e-mailed to those people subscribed to the Newsletter list (http://www.rcsb.org/pdb/forum.html). A printed Annual Report will summarize the past year's events. *PDB at the Biophysical Society Annual Meeting The PDB will have an exhibition booth at the Biophysical Society 2000 Meeting in New Orleans, LA from February 13-15. Please stop by and say hello! *New Building for the RU PDB Team The PDB team at Rutgers University has moved into a newly renovated building located on Rutgers University's Busch Campus. The new space has expanded office room for the annotation and programming staff and a specialized high-density filing system for the maintenance of the physical archive by the PDB. The new conference room was the location of the opening dedication ceremony, which was attended by Rutgers administration, faculty, and students. *One-year Anniversary of RCSB PDB Grant Award October 1, 1999 marked the passing of one year since the RCSB was awarded the grant to manage the Protein Data Bank (PDB). We are pleased to report that our initial goals have been met, and in many cases surpassed, with the help and support of the PDB community. Of special note is the fact that this date was originally intended to mark the end of the transition period -- a date that actually occurred on July 1, 1999 -- when the RCSB assumed the full responsibility for all PDB activities. In addition to maintaining the PDB distribution activities, the PDB introduced new tools for deposition and query, including -ADIT (AutoDep Input Tool - http://pdb.rutgers.edu/adit/), which has proven to be a very useful tool for both data deposition and annotation. Structures deposited using ADIT are fully processed to completion in an average of nine days. -SearchLite (http://www.rcsb.org/pdb/searchlite.html) and SearchFields (http://www.rcsb.org/pdb/cgi/queryForm.cgi), which provide simple and advanced searching of the PDB. Search results are examined using the Structure Explorer and the Query Result interfaces. ----------------------------------------- DATA DEPOSITION, PROCESSING, AND UNIFORMITY *Summary From January 27, 1999 - December 1, 1999, 1994 structures were deposited with the RCSB. On average, these structures are processed and returned to the author in less than ten days. The 458 structures inherited by the RCSB from the Brookhaven National Laboratory (BNL) backlog have been processed, sent to the author, and are being released according to their release status. The 456 Layer 1 entries brought to Layer 2 status have been processed, sent to the author, and released into the PDB archive. Overall, approximately 25% of the current PDB archive has been processed by the RCSB PDB. Preparations for another ADIT site have begun at the Institute for Protein Research in Osaka, Japan. Members from the PDB went to Osaka in September to set up the mirror equipment, and members from Osaka came to Rutgers University in December for ADIT training. This site will be available shortly. ADIT's extension dictionaries are also being developed. With the BIOSYNC project, the PDB is developing an extension dictionary and input tool that enables ADIT to collect the data required to describe synchotron facilities and beamlines. The revisions and extensions from the NMR task force are also being integrated into the NMR view of ADIT. The Data Uniformity project, whose goal is to make the archive as consistent and error-free as possible, is being accomplished by using two methods. The file-by-file method examines entries individually using ADIT. These entries are processed in groups of similar structures to insure the consistency of these data. The archival data are also being processed by looking across the archive at specific tables. Using this batch method, we have completed the tables for R-factor, resolution, and citation information. These updated tables will be implemented for use with the SearchLite and SearchFields tools, and will be available early in the year 2000. Currently, uniformity efforts are being focused on items such as source, enzyme classification, and compound. *Completion of Layer 1 Entry Processing Starting in mid-December, the release of the fully processed versions of the PDB Entries currently classified as "Layer 1" began. The Layer 1 entries were annotated and processed over the summer by the PDB Processing Team. These entries have a REVDAT record with a modification number of 4 to reflect the change from Layer 1 to Layer 2, as was indicated in the PDB format advisory that is described in this newsletter. In addition, several PDB format records are to be included in the REVDAT to further indicate that the file has been brought to Layer 2 status. REVDAT 2 15-DEC-99 1ABC 4 HEADER COMPND REMARK JRNL REVDAT 2 2 4 ATOM SOURCE SEQRES The Layer 1 upgrade project was undertaken to ensure the uniformity of the data in the archive, and marks the end of Layer 1 releases all together. Any additional changes to these files may be sent to deposit@rcsb.rutgers.edu. *Processing of Backlog Structures Completed Processing of the backlog structures inherited at the time of the PDB transition has been completed by the RCSB. These 458 structures arrived at the RCSB PDB in various stages of processing on July 1, 1999. Several structures had been processed almost to completion by the Brookhaven National Laboratory staff, and were quickly run through final checks and modifications by the RCSB PDB staff. Other structures were fully processed by the RCSB PDB annotators and the RCSB PDB summer staff. The RCSB brought together a team to tackle the backlog structures: Nathaniel Macapagal and Michael Huang, two recent Rutgers graduates who each spent two years doing undergraduate research with the NDB, including the NDB Undergraduate Research Experience Summer Program; Andrew Napoli, a Rutgers graduate student in chemistry; and Tara Abrams, a Princeton University biochemistry undergraduate. After a period of training directed by senior annotator Shri Jain, the RCSB PDB summer staff used ADIT, the AutoDep Input Tool (http://pdb.rutgers.edu/adit/), to process and annotate these structures. Every file was further reviewed by the annotator staff including Kyle Burkhardt, Victoria Colflesh, and Bohdan Schneider. The processed backlog structures were sent to the authors for review, and are being released according to the instructions provided by the author. The RCSB-Rutgers team has also brought the 456 structures that were released as "Layer 1" -- meaning unannotated and unprocessed -- up to "Layer 2" status. This will ensure the uniformity of the data in the archive and marks the end of "Layer 1" files all together. Authors of these files will be contacted in the next week for final approval of the "Layer 2" release. *PDB Format Changes Implemented Starting November 1999, all files released by the PDB will reflect the changes listed below. A full description and explanation is available in the summary of comments on PDB Change Advisory Notice (http://www.rcsb.org/pdb/lists/pdb-l/199910/msg00012.html) that was announced on October 13, 1999. - The REMARK 500 outlier criteria is 6*RMSD to flag bond distance and bond angle outliers. - The REMARK 300 and 350 templates have improved descriptions for biological assemblies. - Files will continue to be placed into the PDB archive with the current hydrogen atom nomenclature. In the near future, files with IUPAC-compliant nomenclature will be placed in a separate area on the ftp server. These files will carry new REMARK records that clearly and prominently identify these files as having a IUPAC hydrogen atom nomenclature. Details about the location of the new files will be announced shortly. - The COMPND record will simplify/clarify the specification of macromolecule content. - The REVDAT modification type (modType) is used to indicate if a structure is a re-released Layer 1 entry (with a modification type of 4) or whether it is a re-released entry that has been modified as part of the data uniformity project (with a modification type of 5). For example, the following REVDAT is for a structure that has been re-released as a Layer 2 file after being initially released as a Layer 1. REVDAT 2 03-NOV-99 1ABC 4 The following REVDAT is for a structure that has been modified as part of the data uniformity project. REVDAT 2 03-NOV-99 1BER 5 - CODEN identifiers are not included in files released on or after November 3, 1999. ----------------------------------------- DATA DISTRIBUTION AND QUERY Data in the form of PDB files continue to be distributed once per week and become available no later than 1:00 am PST each Wednesday. Mirror sites have been established (see below for details) and are updated along with the main PDB site. Additional mirrors are also under development. Tools for query and reporting are being enhanced. *New CD-ROM of PDB Holdings Now Available Issue 90 of the PDB CD-ROM Sets, which includes PDB structures deposited before October 1, 1999, is now available for on-line orders (http://www.rcsb.org/pdb/cdrom.html). In addition to ordering Issue 90, you can place orders for the four issues of 2000. The CD-ROM sets are provided at no charge. Issue 91 will be available by February 2000. With Issue 90, all the NMR Constraint files have been added to the CD-ROMs; the PDB Newsletter is also included in both text and PDF format. *PDB Opens Beta Test Site The PDB has opened a beta test site (http://beta.rcsb.org/pdb/) in order to present new developments to the user community for evaluation before they appear on the other public PDB sites. All PDB users are invited to participate in testing the new features that appear on this site. The beta site is intended to provide users with a first glimpse of new features (http://www.rcsb.org/pdb/beta.html) as they are being developed, but these features may not be robust as the applications on the production sites. Establishing the dependability of these new applications is part of the testing process! Please also note that the performance of the beta site is expected to vary and may not approach that of the production sites. The current content of the beta site is documented here. This first release of the beta site contains the First Glance and Protein Explorer (http://www.umass.edu/microbio/chime/explorer/) applications developed by Eric Martz (emartz@microbio.umass.edu). Please e-mail any comments or bug reports to notify@rcsb.org. *New RCSB PDB Mirrors New RCSB PDB mirrors have been established: Cambridge Crystallographic Data http://pdb.ccdc.cam.ac.uk/ Center, United Kingdom Osaka University, Japan http://pdb.protein.osaka-u.ac.jp/ National University of Singapore, http://pdb.bic.nus.edu.sg/ Singapore All RCSB PDB mirrors sites are listed at http://www.rcsb.org/pdb/mirrors.html. * Redirection of Traffic from the BNL PDB Site Since the shutdown of the BNL PDB site on June 30, 1999, any user attempting to access the BNL PDB Web or FTP addresses has been automatically redirected to the appropriate RCSB PDB address. Although we will endeavor to keep this redirection in place as long as possible, this matter is not entirely under our control, and there is a possibility that it may cease in the near future. We strongly recommend that all users of the PDB update any links in Web documents, software, and personal bookmarks at this time so as to avoid the possibility of a discontinuity in service. * Recent Additions to the PDB Web Site Documentation and information provided on the PDB Web site is constantly evolving in response to user requests and questions received through the PDB help service. Significant recent additions to the Web site are the provision of data on the growth of the PDB (http://www.rcsb.org/pdb/holdings.html), the education page (http://www.rcsb.org/pdb/education.html), and the list of new features (http://www.rcsb.org/pdb/beta.html) to try on the beta test site (http://beta.rcsb.org/pdb/). * New Visualization Applications on the PDB Web Site The First Glance and Protein Explorer applications (http://www.umass.edu/microbio/chime/explorer/), initially provided on the PDB beta site (http://beta.rcsb.org/pdb/), have now been added to the production Web sites. Both of these applications are implemented as View Structure options on the Structure Explorer pages and make use of Chemscape Chime (http://www.mdli.com/support/chime/default.html). A description of these new applications is available at: http://www.rcsb.org/pdb/beta.html. ----------------------------------------- OUTREACH ACTIVITIES * Two Workshops on Structural Genomics at Argonne National Laboratory by Andrzej Joachimiak and John Westbrook The PDB actively participated in two workshops held at Argonne National Laboratory in November. These workshops brought together researchers who are actively working in the fields of bioinformatics, high throughput cloning, expression and purification, protein crystallization, protein structure determination and validation, protein structure prediction, data bases - important fields in a "pipeline" that proceed from gene sequence to protein structure. In the first workshop entitled, "High Throughput Methods for Structural Genomics", Helen Berman presented the PDB plans for enabling structural genomics. The second workshop: "Rapid Structure Determination at 3rd Generation Synchrotron Sources", was intended to experimentally demonstrate the unique capabilities at 3rd generation sources through a hands-on data collection, analysis, and structure determination. The experimental part was complemented by short seminars and discussion with experts. John Westbrook gave a practical demonstration of the PDB plans for enabling structural genomics. Under an expert guide, attendees worked as teams to collect and process diffraction data, learn how to analyze and evaluate data, solve a structure using MAD analysis, automatically build a model, and refine a structure. Several alternative crystallographic software packages were used at the beamline for data processing, analysis and structure determination. High-throughput methods were emphasized. MAD data were collected on four SeMet labeled proteins ranging from simple (16 kDa) to challenging (450 kDa) problems. All four structures were solved from the data collected during the three-day workshop. The purpose of the demonstration was also to illustrate how data pipelining -- the incremental collection of data required for PDB deposition -- could be used to automate virtually all aspects of the PDB deposition. Although the demonstration was focused on collecting information for deposition, the same techniques can be applied to collect and encode experimental details that need to be captured during high throughput experiments. Prior to the workshop, we worked with Wladek Minor and Zbyszek Otwinowski to develop an mmCIF extension dictionary to capture key data items from the data processing program HKL2000. This allowed us to collect information during data processing that could be pipelined directly into the deposition process. In prior work with the developers of X-PLOR and CNS, we had developed a macro to export the details of refinement in mmCIF format. During the workshop, we demonstrated the full cycle of data collection, processing, refinement, and deposition at PDB for two protein systems: peptide binding domain of thermophilic chaperonin and aldose reductase. The MAD data sets were processed using HKL2000. MAD phasing was accomplished with SOLVE, CNS and SHARP, autotracing with ARP/wARP. Refinement was performed using CNS. Details of each step in the structure determination were electronically captured and integrated with the coordinates of the deposited structure. The success of this demonstration and the lessons that were learned will enable us to further improve our systems so that PDB deposition will become fully integrated into high throughput structure determination. * New Web Site for Synchrotron Users A new Web site is in place to put detailed information on US synchrotron beamlines for macromolecular crystallography at researchers' fingertips. The National Institutes of Health Division of Research Resources (NCRR - http://www.ncrr.nih.gov/) has funded the National Biomedical Computation Resource (NBCR - http://www.sdsc.edu/nbcr), centered at the San Diego Supercomputer Center (http://www.sdsc.edu), to develop a Web site for BioSync, the Structural Biology Synchrotron Users Organization. The site, http://biosync.sdsc.edu, began operating over the summer as a portal for investigators planning visits to synchrotron facilities and as a central resource for researchers in structural biology seeking information about such facilities. Over the past ten years, the importance of high-energy synchrotron beams in x-ray crystallography has grown rapidly, BioSync research reveals. In 1990, a survey estimated that about 18% of publications of new structures reported use of synchrotron radiation. In the early 1990s, that figure jumped to 25%. New synchrotron facilities and widespread adoption of cryopreservation of crystals led to an increase in synchrotron dependence to 49% of new structure publications in 1998. The new Web site addresses an urgent need for a central location with information on synchrotron beamlines for macromolecular crystallography. Along with researchers' growing dependence on synchrotron radiation, the number of new beamlines is expanding rapidly, and operating beamlines are being upgraded continuously. The intent of the Web site is to demystify what has been a word-of-mouth process and help to rationalize and democratize the use of the facilities. A BioSync committee outlined the new Web site to include information on the capabilities and instrumentation of each beamline, as well as key staff, access procedures, and pointers to local Web sites. The intent is to give users a single point of entry to obtain information about US beamlines for crystallography. The BioSync committee included Daved Fremont (Washington University), Osnat Herzberg (University of Maryland), Alfonso Mondragon (Northwestern University), Dan Thiel (Cornell University), John Badger (San Diego Supercomputing Center) and Janet Smith (Purdue University). The Structural Biology Synchrotron Users Organization (BioSync) was formed in 1990 to promote access to synchrotron radiation for scientists whose primary research is in the field of structural biology and who use synchrotron radiation for experiments in crystallography, spectroscopy, scattering from noncrystalline materials and imaging. The BioSync membership includes leaders of all such structural biology research groups in North America. Further information on BioSync is available from a report developed to evaluate the existing resources and future needs for synchrotron radiation. The NCRR supports the research infrastructure of many shared facilities, including stations for crystallography at Cornell, Stanford, Brookhaven, Argonne and NBCR. *Hand-held Models of Proteins The opportunity exists for the PDB, thanks to the National Science Foundation (http://www.nsf.gov) sponsored TeleManufacturing Facility (http://www.sdsc.edu/tmf) at the San Diego Supercomputer Center (http://www.sdsc.edu/) directed by Mike Bailey (mjb@sdsc.edu), to produce and distribute free approximately six solid models of proteins each week. The sturdy models produced on a rapid prototyping device are approximately 4-6 inches and show good surface detail. The process and results for studying virus assemblies and light harvesting complexes were described in Bailey, Schulten, and Johnson, Current Opinions in Structural Biology 8(2), 202-208 (1998). The models are constructed from a surface representation produced from programs like Grasp (http://trantor.bioc.columbia.edu/grasp/) (or in this case, the UNC SURF program and VMD [http://www.ks.uiuc.edu/Research/vmd/]). It is only necessary to provide a PDB id or Cartesian coordinates in PDB format for the model to be constructed. We are interested in your feedback on the appropriate models to make and for whom. Input to the following questions is sought. 1.Would you find such models useful? If so why? 2.Should we provide models to: - instructors of credited courses? or - structure depositors who deposit a protein with a potentially new fold? or - specific requests justified by a description of why a good model of the surface is important? 3.What proteins do you see as particularly good candidates? 4.Other comments? Please reply to the PDB discussion list (http://www.rcsb.org/pdb/forum.html). Live camera shots of the TeleManufacturing Facility can be viewed at http://www.sdsc.edu/tmf/LomCam/lomcam.html. * RFP for CORBA Standard for Biological Macromolecular Structure Data For the past several months, RCSB has been active in the Object Management Group (OMG), the non-profit organization responsible for the Common Object Request Broker Architecture (CORBA). CORBA is widely used in the computer industry for developing object-oriented network interfaces that are independent of hardware platform, operating system and programming language. SDSC as a member of the OMG has been representing the RCSB in its effort to define a specification for accessing the PDB using CORBA. A major milestone was recently achieved in this process, as described in the following excerpt from a press release issued by the OMG: "The Domain Technology Committee (DTC), responsible for the OMG's specification efforts in vertical markets, issued a Request For Proposals (RFP) for a biological macromolecular structure data access facility. The facility will find uses in both academic and commercial biochemical research and development." The full version of the RFP is available online: http://www.omg.org/cgi-bin/doc?lifesci/99-08-15. ----------------------------------------- STATEMENT OF SUPPORT The PDB is supported by funds from the National Science Foundation, the Office of Biology and Environmental Research at the Department of Energy, and two units of the National Institutes of Health: The National Institute of General Medical Sciences and the National Library of Medicine, in addition to resources and staff made available by the host institutions. ----------------------------------------- RCSB PDB TEAM Rutgers, The State University of New Jersey Department of Chemistry Rutgers University 610 Taylor Road Piscataway, NJ 08854-8087 Dr. Helen M. Berman 732-445-4667 Fax: 732-445-4320 berman@rcsb.rutgers.edu Dr. John Westbrook 732-445-4290 Fax: 732-445-4320 jwest@rcsb.rutgers.edu Kyle Burkhardt 732-445-0103 Fax: 732-445-4320 kburkhar@rcsb.rutgers.edu Victoria Colflesh 732-445-0103 Fax: 732-445-4320 victoria@rcsb.rutgers.edu Dr. Zukang Feng 732-445-0103 Fax: 732-445-4320 zfeng@rcsb.rutgers.edu Michael Huang 732.445.0103 Fax: 732.445.4320 mshuang@rcsb.rutgers.edu Dr. Shri Jain 732-445-0103 Fax: 732-445-4320 sjain@rcsb.rutgers.edu Dr. Rachel Kramer 732-445-0103 Fax: 732-445-4320 kramer@rcsb.rutgers.edu Dr. Bohdan Schneider 732-445-0103 Fax: 732-445-4320 bohdan@rcsb.rutgers.edu Dr. Kata Schneider 732-445-0103 Fax: 732-445-4320 kata@rcsb.rutgers.edu Christine Zardecki 732-445-0103 Fax: 732-445-4320 zardecki@rcsb.rutgers.edu NIST Biotechnology Division National Institute of Standards and Technology Gaithersburg, MD 20899-8310 Dr. Gary Gilliland 301-975-2629 Fax: 301-330-3447 gary.gilliland@nist.gov Dr. Talapady N. Bhat 301-975-8702 Fax: 301-975-8717 talapady.bhat@nist.gov Phoebe Fagan 301-975-2213 Fax: 301-975-8717 phoebe.fagan@nist.gov Dr. Diane Hancock 301-975-4873 Fax: 301-975-8717 diane.hancock@nist.gov Dr. Narmada Thanki 301-975-8704 Fax: 301-975-8717 narmada@carb.nist.gov Dr. Michael Tung 301-975-5328 Fax: 301-975-8717 michael.tung@nist.gov Dr. Greg Vasquez 301-975-4195 Fax: 301-975-8717 gregory.vasquez@nist.gov UCSD/SDSC SDSC UC San Diego 9500 Gilman Drive La Jolla, CA 92093 Dr. Peter Arzberger 858-534-5079 Fax: 858-822-0948 parzberg@sdsc.edu Dr. Phil Bourne 858-534-8301 Fax: 858-822-0873 bourne@sdsc.edu Dr. John Badger 858-822-0936 Fax: 858-822-0873 badger@sdsc.edu Dr. Douglas S. Greer 858-534-8357 Fax: 858-534-5113 dsg@sdsc.edu Dr. Michael Gribskov 858-534-8312 Fax: 858-822-0873 gribskov@sdsc.edu Dorothy Kegler 858-822-0880 Fax: 858-822-0873 dkegler@sdsc.edu Dr. John Kowalski 858-822-0897 Fax: 858-822-5113 kowalski@sdsc.edu Shawn Strande 858-534-5133 Fax: 858-534-5117 strande@sdsc.edu Dr. Lynn F. Ten Eyck 858-534-5141 Fax: 858-534-5113 teneyckl@sdsc.edu Dr. Helge Weissig 858-534-8399 Fax: 858-822-0873 helgew@sdsc.edu Dr. Kenneth Yoshimoto 858-822-0886 Fax: 858-822-0873 kenneth@sdsc.edu ----------------------------------------- PDB Newsletter Number 4 -- Winter, 1999 -- RCSB Published quarterly by the Research Collaboratory for Structural Bioinformatics Weekly PDB news is available on the Web at http://www.rcsb.org/pdb/latest_news.html. This newsletter will soon be available on the Web in HTML format at http://www.rcsb.org/pdb/newsletter/