data_26525 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 26525 _Entry.Title ; Structure, Dynamics, and Kinetics of Weak Protein Protein Complexes from NMR Spin Relaxation Measurements of Titrated Solutions ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2015-02-26 _Entry.Accession_date 2015-02-26 _Entry.Last_release_date 2015-03-11 _Entry.Original_release_date 2015-03-11 _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 3.1 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 Loic Salmon . . . 26525 2 Jose-Luis 'Ortega Roldan' . . . 26525 3 Ewen Lescop . . . 26525 4 Antoine Licinio . . . 26525 5 Nico 'van Nuland' . . . 26525 6 Malene Jensen . R. . 26525 7 Martin Blackledge . . . 26525 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID heteronucl_T1_relaxation 8 26525 heteronucl_T2_relaxation 8 26525 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'T1 relaxation values' 456 26525 'T2 relaxation values' 456 26525 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2015-03-11 2015-02-26 original author . 26525 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 26525 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 21425222 _Citation.Full_citation . _Citation.Title 'Structure, dynamics, and kinetics of weak protein-protein complexes from NMR spin relaxation measurements of titrated solutions.' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Angew. Chem. Int. Ed. Engl.' _Citation.Journal_name_full . _Citation.Journal_volume 50 _Citation.Journal_issue 16 _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 3755 _Citation.Page_last 3759 _Citation.Year 2011 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Loic Salmon . . . 26525 1 2 Jose-Luis 'Ortega Roldan' . . . 26525 1 3 Ewen Lescop . . . 26525 1 4 Antoine Licinio . . . 26525 1 5 Nico 'van Nuland' . . . 26525 1 6 Malene Jensen . R. . 26525 1 7 Martin Blackledge . . . 26525 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 26525 _Assembly.ID 1 _Assembly.Name 'CD2AP SH3 domain complex with Ubiquitin' _Assembly.BMRB_code . _Assembly.Number_of_components . _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds . _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'CD2AP SH3 Domain' 1 $SH3_domain A . yes native no no . . . 26525 1 2 Ubiquitin 2 $Ubiquitin B . yes native no no . . . 26525 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_SH3_domain _Entity.Sf_category entity _Entity.Sf_framecode SH3_domain _Entity.Entry_ID 26525 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name SH3_domain _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; GKIKAKEYCRTLFAYEGTNN DELTFKEGEIIHLISKETGE AGWWRGELNGKEGVFPDNFA VQINE ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 65 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-11-25 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no GB EGW10641 . "putative G-protein coupled receptor 111 [Cricetulus griseus]" . . . . . 53.85 1424 97.14 97.14 1.82e-13 . . . . 26525 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 1 GLY . 26525 1 2 2 LYS . 26525 1 3 3 ILE . 26525 1 4 4 LYS . 26525 1 5 5 ALA . 26525 1 6 6 LYS . 26525 1 7 7 GLU . 26525 1 8 8 TYR . 26525 1 9 9 CYS . 26525 1 10 10 ARG . 26525 1 11 11 THR . 26525 1 12 12 LEU . 26525 1 13 13 PHE . 26525 1 14 14 ALA . 26525 1 15 15 TYR . 26525 1 16 16 GLU . 26525 1 17 17 GLY . 26525 1 18 18 THR . 26525 1 19 19 ASN . 26525 1 20 20 ASN . 26525 1 21 21 ASP . 26525 1 22 22 GLU . 26525 1 23 23 LEU . 26525 1 24 24 THR . 26525 1 25 25 PHE . 26525 1 26 26 LYS . 26525 1 27 27 GLU . 26525 1 28 28 GLY . 26525 1 29 29 GLU . 26525 1 30 30 ILE . 26525 1 31 31 ILE . 26525 1 32 32 HIS . 26525 1 33 33 LEU . 26525 1 34 34 ILE . 26525 1 35 35 SER . 26525 1 36 36 LYS . 26525 1 37 37 GLU . 26525 1 38 38 THR . 26525 1 39 39 GLY . 26525 1 40 40 GLU . 26525 1 41 41 ALA . 26525 1 42 42 GLY . 26525 1 43 43 TRP . 26525 1 44 44 TRP . 26525 1 45 45 ARG . 26525 1 46 46 GLY . 26525 1 47 47 GLU . 26525 1 48 48 LEU . 26525 1 49 49 ASN . 26525 1 50 50 GLY . 26525 1 51 51 LYS . 26525 1 52 52 GLU . 26525 1 53 53 GLY . 26525 1 54 54 VAL . 26525 1 55 55 PHE . 26525 1 56 56 PRO . 26525 1 57 57 ASP . 26525 1 58 58 ASN . 26525 1 59 59 PHE . 26525 1 60 60 ALA . 26525 1 61 61 VAL . 26525 1 62 62 GLN . 26525 1 63 63 ILE . 26525 1 64 64 ASN . 26525 1 65 65 GLU . 26525 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . GLY 1 1 26525 1 . LYS 2 2 26525 1 . ILE 3 3 26525 1 . LYS 4 4 26525 1 . ALA 5 5 26525 1 . LYS 6 6 26525 1 . GLU 7 7 26525 1 . TYR 8 8 26525 1 . CYS 9 9 26525 1 . ARG 10 10 26525 1 . THR 11 11 26525 1 . LEU 12 12 26525 1 . PHE 13 13 26525 1 . ALA 14 14 26525 1 . TYR 15 15 26525 1 . GLU 16 16 26525 1 . GLY 17 17 26525 1 . THR 18 18 26525 1 . ASN 19 19 26525 1 . ASN 20 20 26525 1 . ASP 21 21 26525 1 . GLU 22 22 26525 1 . LEU 23 23 26525 1 . THR 24 24 26525 1 . PHE 25 25 26525 1 . LYS 26 26 26525 1 . GLU 27 27 26525 1 . GLY 28 28 26525 1 . GLU 29 29 26525 1 . ILE 30 30 26525 1 . ILE 31 31 26525 1 . HIS 32 32 26525 1 . LEU 33 33 26525 1 . ILE 34 34 26525 1 . SER 35 35 26525 1 . LYS 36 36 26525 1 . GLU 37 37 26525 1 . THR 38 38 26525 1 . GLY 39 39 26525 1 . GLU 40 40 26525 1 . ALA 41 41 26525 1 . GLY 42 42 26525 1 . TRP 43 43 26525 1 . TRP 44 44 26525 1 . ARG 45 45 26525 1 . GLY 46 46 26525 1 . GLU 47 47 26525 1 . LEU 48 48 26525 1 . ASN 49 49 26525 1 . GLY 50 50 26525 1 . LYS 51 51 26525 1 . GLU 52 52 26525 1 . GLY 53 53 26525 1 . VAL 54 54 26525 1 . PHE 55 55 26525 1 . PRO 56 56 26525 1 . ASP 57 57 26525 1 . ASN 58 58 26525 1 . PHE 59 59 26525 1 . ALA 60 60 26525 1 . VAL 61 61 26525 1 . GLN 62 62 26525 1 . ILE 63 63 26525 1 . ASN 64 64 26525 1 . GLU 65 65 26525 1 stop_ save_ save_Ubiquitin _Entity.Sf_category entity _Entity.Sf_framecode Ubiquitin _Entity.Entry_ID 26525 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name Ubiquitin _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; HHHHHHMQIFVKTLTGKTIT LEVEPSDTIENVKAKIQDKE GIPPDQQRLIFAGKQLEDGR TLSDYNIQKESTLHLVLRLR GG ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details 'Ubiquitin contains a His-tag (6 successive Histidines)' _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 82 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 -5 HIS . 26525 2 2 -4 HIS . 26525 2 3 -3 HIS . 26525 2 4 -2 HIS . 26525 2 5 -1 HIS . 26525 2 6 0 HIS . 26525 2 7 1 MET . 26525 2 8 2 GLN . 26525 2 9 3 ILE . 26525 2 10 4 PHE . 26525 2 11 5 VAL . 26525 2 12 6 LYS . 26525 2 13 7 THR . 26525 2 14 8 LEU . 26525 2 15 9 THR . 26525 2 16 10 GLY . 26525 2 17 11 LYS . 26525 2 18 12 THR . 26525 2 19 13 ILE . 26525 2 20 14 THR . 26525 2 21 15 LEU . 26525 2 22 16 GLU . 26525 2 23 17 VAL . 26525 2 24 18 GLU . 26525 2 25 19 PRO . 26525 2 26 20 SER . 26525 2 27 21 ASP . 26525 2 28 22 THR . 26525 2 29 23 ILE . 26525 2 30 24 GLU . 26525 2 31 25 ASN . 26525 2 32 26 VAL . 26525 2 33 27 LYS . 26525 2 34 28 ALA . 26525 2 35 29 LYS . 26525 2 36 30 ILE . 26525 2 37 31 GLN . 26525 2 38 32 ASP . 26525 2 39 33 LYS . 26525 2 40 34 GLU . 26525 2 41 35 GLY . 26525 2 42 36 ILE . 26525 2 43 37 PRO . 26525 2 44 38 PRO . 26525 2 45 39 ASP . 26525 2 46 40 GLN . 26525 2 47 41 GLN . 26525 2 48 42 ARG . 26525 2 49 43 LEU . 26525 2 50 44 ILE . 26525 2 51 45 PHE . 26525 2 52 46 ALA . 26525 2 53 47 GLY . 26525 2 54 48 LYS . 26525 2 55 49 GLN . 26525 2 56 50 LEU . 26525 2 57 51 GLU . 26525 2 58 52 ASP . 26525 2 59 53 GLY . 26525 2 60 54 ARG . 26525 2 61 55 THR . 26525 2 62 56 LEU . 26525 2 63 57 SER . 26525 2 64 58 ASP . 26525 2 65 59 TYR . 26525 2 66 60 ASN . 26525 2 67 61 ILE . 26525 2 68 62 GLN . 26525 2 69 63 LYS . 26525 2 70 64 GLU . 26525 2 71 65 SER . 26525 2 72 66 THR . 26525 2 73 67 LEU . 26525 2 74 68 HIS . 26525 2 75 69 LEU . 26525 2 76 70 VAL . 26525 2 77 71 LEU . 26525 2 78 72 ARG . 26525 2 79 73 LEU . 26525 2 80 74 ARG . 26525 2 81 75 GLY . 26525 2 82 76 GLY . 26525 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . HIS 1 1 26525 2 . HIS 2 2 26525 2 . HIS 3 3 26525 2 . HIS 4 4 26525 2 . HIS 5 5 26525 2 . HIS 6 6 26525 2 . MET 7 7 26525 2 . GLN 8 8 26525 2 . ILE 9 9 26525 2 . PHE 10 10 26525 2 . VAL 11 11 26525 2 . LYS 12 12 26525 2 . THR 13 13 26525 2 . LEU 14 14 26525 2 . THR 15 15 26525 2 . GLY 16 16 26525 2 . LYS 17 17 26525 2 . THR 18 18 26525 2 . ILE 19 19 26525 2 . THR 20 20 26525 2 . LEU 21 21 26525 2 . GLU 22 22 26525 2 . VAL 23 23 26525 2 . GLU 24 24 26525 2 . PRO 25 25 26525 2 . SER 26 26 26525 2 . ASP 27 27 26525 2 . THR 28 28 26525 2 . ILE 29 29 26525 2 . GLU 30 30 26525 2 . ASN 31 31 26525 2 . VAL 32 32 26525 2 . LYS 33 33 26525 2 . ALA 34 34 26525 2 . LYS 35 35 26525 2 . ILE 36 36 26525 2 . GLN 37 37 26525 2 . ASP 38 38 26525 2 . LYS 39 39 26525 2 . GLU 40 40 26525 2 . GLY 41 41 26525 2 . ILE 42 42 26525 2 . PRO 43 43 26525 2 . PRO 44 44 26525 2 . ASP 45 45 26525 2 . GLN 46 46 26525 2 . GLN 47 47 26525 2 . ARG 48 48 26525 2 . LEU 49 49 26525 2 . ILE 50 50 26525 2 . PHE 51 51 26525 2 . ALA 52 52 26525 2 . GLY 53 53 26525 2 . LYS 54 54 26525 2 . GLN 55 55 26525 2 . LEU 56 56 26525 2 . GLU 57 57 26525 2 . ASP 58 58 26525 2 . GLY 59 59 26525 2 . ARG 60 60 26525 2 . THR 61 61 26525 2 . LEU 62 62 26525 2 . SER 63 63 26525 2 . ASP 64 64 26525 2 . TYR 65 65 26525 2 . ASN 66 66 26525 2 . ILE 67 67 26525 2 . GLN 68 68 26525 2 . LYS 69 69 26525 2 . GLU 70 70 26525 2 . SER 71 71 26525 2 . THR 72 72 26525 2 . LEU 73 73 26525 2 . HIS 74 74 26525 2 . LEU 75 75 26525 2 . VAL 76 76 26525 2 . LEU 77 77 26525 2 . ARG 78 78 26525 2 . LEU 79 79 26525 2 . ARG 80 80 26525 2 . GLY 81 81 26525 2 . GLY 82 82 26525 2 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 26525 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $SH3_domain . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . . . . . . . . . 26525 1 2 2 $Ubiquitin . 9606 organism . 'Homo sapiens' Human . . Eukaryota Metazoa Homo sapiens . . . . . . . . . . . . . . . . . . . . . 26525 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 26525 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $SH3_domain . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . . . . . . . . . . . . . . BL21(DE3) . . . . . . 26525 1 2 2 $Ubiquitin . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli . . . . . . . . . . . . . . . . BL21(DE3) . . . . . . 26525 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_SH3_free _Sample.Sf_category sample _Sample.Sf_framecode sample_SH3_free _Sample.Entry_ID 26525 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '92% H2O/8% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 SH3_domain '[U-100% 15N]' . . 1 $SH3_domain . . 0.250 . . mM . . . . 26525 1 2 'sodium phosphate' 'natural abundance' . . . . . . 50 . . mM . . . . 26525 1 3 DTT 'natural abundance' . . . . . . 1 . . mM . . . . 26525 1 4 D2O 'natural abundance' . . . . . . 8 . . % . . . . 26525 1 5 H2O 'natural abundance' . . . . . . 92 . . % . . . . 26525 1 stop_ save_ save_sample_Ubiquitin_free _Sample.Sf_category sample _Sample.Sf_framecode sample_Ubiquitin_free _Sample.Entry_ID 26525 _Sample.ID 2 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '92% H2O/8% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 Ubiquitin '[U-100% 15N]' . . 2 $Ubiquitin . . 0.250 . . mM . . . . 26525 2 2 'sodium phosphate' 'natural abundance' . . . . . . 50 . . mM . . . . 26525 2 3 DTT 'natural abundance' . . . . . . 1 . . mM . . . . 26525 2 4 D2O 'natural abundance' . . . . . . 8 . . % . . . . 26525 2 5 H2O 'natural abundance' . . . . . . 92 . . % . . . . 26525 2 stop_ save_ save_sample_mixture_p1 _Sample.Sf_category sample _Sample.Sf_framecode sample_mixture_p1 _Sample.Entry_ID 26525 _Sample.ID 3 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '92% H2O/8% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'Ubiquitin Free' '[U-100% 15N]' . . 2 $Ubiquitin . . 0.173 . . mM . . . . 26525 3 2 'SH3 Free' '[U-100% 15N]' . . 1 $SH3_domain . . 0.554 . . mM . . . . 26525 3 3 'sodium phosphate' 'natural abundance' . . . . . . 50 . . mM . . . . 26525 3 4 DTT 'natural abundance' . . . . . . 1 . . mM . . . . 26525 3 5 D2O 'natural abundance' . . . . . . 8 . . % . . . . 26525 3 6 H2O 'natural abundance' . . . . . . 92 . . % . . . . 26525 3 stop_ save_ save_sample_mixture_p2 _Sample.Sf_category sample _Sample.Sf_framecode sample_mixture_p2 _Sample.Entry_ID 26525 _Sample.ID 4 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '92% H2O/8% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'Ubiquitin Free' '[U-100% 15N]' . . 2 $Ubiquitin . . 0.231 . . mM . . . . 26525 4 2 'SH3 Free' '[U-100% 15N]' . . 1 $SH3_domain . . 0.345 . . mM . . . . 26525 4 3 'sodium phosphate' 'natural abundance' . . . . . . 50 . . mM . . . . 26525 4 4 DTT 'natural abundance' . . . . . . 1 . . mM . . . . 26525 4 5 D2O 'natural abundance' . . . . . . 8 . . % . . . . 26525 4 6 H2O 'natural abundance' . . . . . . 92 . . % . . . . 26525 4 stop_ save_ save_sample_mixture_p3 _Sample.Sf_category sample _Sample.Sf_framecode sample_mixture_p3 _Sample.Entry_ID 26525 _Sample.ID 5 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '92% H2O/8% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'Ubiquitin Free' '[U-100% 15N]' . . 2 $Ubiquitin . . 0.387 . . mM . . . . 26525 5 2 'SH3 Free' '[U-100% 15N]' . . 1 $SH3_domain . . 0.185 . . mM . . . . 26525 5 3 'sodium phosphate' 'natural abundance' . . . . . . 50 . . mM . . . . 26525 5 4 DTT 'natural abundance' . . . . . . 1 . . mM . . . . 26525 5 5 D2O 'natural abundance' . . . . . . 8 . . % . . . . 26525 5 6 H2O 'natural abundance' . . . . . . 92 . . % . . . . 26525 5 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 26525 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID temperature 308 . K 26525 1 pH 6.0 . pH 26525 1 pressure 1 . atm 26525 1 stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Software.Sf_category software _Software.Sf_framecode NMRPipe _Software.Entry_ID 26525 _Software.ID 1 _Software.Name NMRPipe _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . 26525 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID processing 26525 1 stop_ save_ save_SPARKY _Software.Sf_category software _Software.Sf_framecode SPARKY _Software.Entry_ID 26525 _Software.ID 2 _Software.Name SPARKY _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID Goddard . . 26525 2 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'data analysis' 26525 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 26525 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Varian _NMR_spectrometer.Model 'NMR Direct-Drive Systems' _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 26525 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Varian 'NMR Direct-Drive Systems' . 600 . . . 26525 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 26525 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '1H-15N HSQC 1' no . . . . . . . . . . 1 $sample_SH3_free isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 26525 1 2 '1H-15N HSQC 2' no . . . . . . . . . . 2 $sample_Ubiquitin_free isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 26525 1 3 '1H-15N HSQC 3' no . . . . . . . . . . 3 $sample_mixture_p1 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 26525 1 4 '1H-15N HSQC 4' no . . . . . . . . . . 4 $sample_mixture_p2 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 26525 1 5 '1H-15N HSQC 5' no . . . . . . . . . . 5 $sample_mixture_p3 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 26525 1 stop_ save_ ######################################## # Heteronuclear T1 relaxation values # ######################################## save_heteronuclear_T1_list_1 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronuclear_T1_list_1 _Heteronucl_T1_list.Entry_ID 26525 _Heteronucl_T1_list.ID 1 _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 600 _Heteronucl_T1_list.T1_coherence_type Sz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details . _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 1 '1H-15N HSQC 1' . . . 26525 1 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 1 1 5 5 ALA N N 15 1.290 0.022 . . . . . 26525 1 2 . 1 1 6 6 LYS N N 15 1.621 0.025 . . . . . 26525 1 3 . 1 1 7 7 GLU N N 15 2.134 0.022 . . . . . 26525 1 4 . 1 1 8 8 TYR N N 15 2.268 0.025 . . . . . 26525 1 5 . 1 1 9 9 CYS N N 15 2.390 0.030 . . . . . 26525 1 6 . 1 1 10 10 ARG N N 15 2.418 0.030 . . . . . 26525 1 7 . 1 1 11 11 THR N N 15 2.429 0.026 . . . . . 26525 1 8 . 1 1 13 13 PHE N N 15 2.282 0.022 . . . . . 26525 1 9 . 1 1 15 15 TYR N N 15 2.298 0.023 . . . . . 26525 1 10 . 1 1 16 16 GLU N N 15 2.175 0.021 . . . . . 26525 1 11 . 1 1 17 17 GLY N N 15 2.236 0.025 . . . . . 26525 1 12 . 1 1 18 18 THR N N 15 2.472 0.026 . . . . . 26525 1 13 . 1 1 19 19 ASN N N 15 2.074 0.017 . . . . . 26525 1 14 . 1 1 20 20 ASN N N 15 2.134 0.027 . . . . . 26525 1 15 . 1 1 21 21 ASP N N 15 2.238 0.020 . . . . . 26525 1 16 . 1 1 23 23 LEU N N 15 2.390 0.037 . . . . . 26525 1 17 . 1 1 24 24 THR N N 15 2.025 0.022 . . . . . 26525 1 18 . 1 1 25 25 PHE N N 15 2.453 0.024 . . . . . 26525 1 19 . 1 1 26 26 LYS N N 15 2.312 0.029 . . . . . 26525 1 20 . 1 1 27 27 GLU N N 15 2.295 0.020 . . . . . 26525 1 21 . 1 1 28 28 GLY N N 15 2.396 0.028 . . . . . 26525 1 22 . 1 1 29 29 GLU N N 15 2.303 0.020 . . . . . 26525 1 23 . 1 1 31 31 ILE N N 15 2.334 0.030 . . . . . 26525 1 24 . 1 1 33 33 LEU N N 15 2.421 0.027 . . . . . 26525 1 25 . 1 1 34 34 ILE N N 15 2.261 0.036 . . . . . 26525 1 26 . 1 1 35 35 SER N N 15 2.297 0.023 . . . . . 26525 1 27 . 1 1 37 37 GLU N N 15 2.164 0.020 . . . . . 26525 1 28 . 1 1 38 38 THR N N 15 2.052 0.030 . . . . . 26525 1 29 . 1 1 39 39 GLY N N 15 2.222 0.032 . . . . . 26525 1 30 . 1 1 40 40 GLU N N 15 2.114 0.017 . . . . . 26525 1 31 . 1 1 41 41 ALA N N 15 2.125 0.020 . . . . . 26525 1 32 . 1 1 43 43 TRP N N 15 2.305 0.026 . . . . . 26525 1 33 . 1 1 45 45 ARG N N 15 2.326 0.031 . . . . . 26525 1 34 . 1 1 46 46 GLY N N 15 2.409 0.029 . . . . . 26525 1 35 . 1 1 47 47 GLU N N 15 2.347 0.025 . . . . . 26525 1 36 . 1 1 48 48 LEU N N 15 2.403 0.031 . . . . . 26525 1 37 . 1 1 49 49 ASN N N 15 2.342 0.042 . . . . . 26525 1 38 . 1 1 50 50 GLY N N 15 2.239 0.026 . . . . . 26525 1 39 . 1 1 51 51 LYS N N 15 2.287 0.019 . . . . . 26525 1 40 . 1 1 53 53 GLY N N 15 2.335 0.024 . . . . . 26525 1 41 . 1 1 54 54 VAL N N 15 2.457 0.029 . . . . . 26525 1 42 . 1 1 55 55 PHE N N 15 2.183 0.026 . . . . . 26525 1 43 . 1 1 57 57 ASP N N 15 2.327 0.023 . . . . . 26525 1 44 . 1 1 58 58 ASN N N 15 2.129 0.020 . . . . . 26525 1 45 . 1 1 59 59 PHE N N 15 2.352 0.033 . . . . . 26525 1 46 . 1 1 60 60 ALA N N 15 2.385 0.027 . . . . . 26525 1 47 . 1 1 61 61 VAL N N 15 2.328 0.024 . . . . . 26525 1 48 . 1 1 63 63 ILE N N 15 2.302 0.032 . . . . . 26525 1 49 . 1 1 64 64 ASN N N 15 1.972 0.016 . . . . . 26525 1 stop_ save_ save_heteronuclear_T1_list_2 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronuclear_T1_list_2 _Heteronucl_T1_list.Entry_ID 26525 _Heteronucl_T1_list.ID 2 _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 600 _Heteronucl_T1_list.T1_coherence_type Sz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details ; Ubiquitin percent bound = 69.5 % SH3 pecent bound = 21.7 % ; _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 3 '1H-15N HSQC 3' . . . 26525 2 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 1 1 5 5 ALA N N 15 1.278 0.037 . . . . . 26525 2 2 . 1 1 6 6 LYS N N 15 1.555 0.030 . . . . . 26525 2 3 . 1 1 7 7 GLU N N 15 2.015 0.008 . . . . . 26525 2 4 . 1 1 8 8 TYR N N 15 2.100 0.008 . . . . . 26525 2 5 . 1 1 9 9 CYS N N 15 2.245 0.011 . . . . . 26525 2 6 . 1 1 10 10 ARG N N 15 2.267 0.009 . . . . . 26525 2 7 . 1 1 11 11 THR N N 15 2.282 0.008 . . . . . 26525 2 8 . 1 1 13 13 PHE N N 15 2.113 0.009 . . . . . 26525 2 9 . 1 1 15 15 TYR N N 15 2.160 0.010 . . . . . 26525 2 10 . 1 1 16 16 GLU N N 15 2.025 0.008 . . . . . 26525 2 11 . 1 1 17 17 GLY N N 15 2.115 0.008 . . . . . 26525 2 12 . 1 1 18 18 THR N N 15 2.306 0.009 . . . . . 26525 2 13 . 1 1 19 19 ASN N N 15 1.974 0.009 . . . . . 26525 2 14 . 1 1 20 20 ASN N N 15 1.990 0.052 . . . . . 26525 2 15 . 1 1 21 21 ASP N N 15 2.087 0.010 . . . . . 26525 2 16 . 1 1 23 23 LEU N N 15 2.197 0.014 . . . . . 26525 2 17 . 1 1 24 24 THR N N 15 1.931 0.007 . . . . . 26525 2 18 . 1 1 25 25 PHE N N 15 2.254 0.007 . . . . . 26525 2 19 . 1 1 26 26 LYS N N 15 2.148 0.009 . . . . . 26525 2 20 . 1 1 27 27 GLU N N 15 2.145 0.007 . . . . . 26525 2 21 . 1 1 28 28 GLY N N 15 2.266 0.012 . . . . . 26525 2 22 . 1 1 29 29 GLU N N 15 2.131 0.009 . . . . . 26525 2 23 . 1 1 31 31 ILE N N 15 2.186 0.009 . . . . . 26525 2 24 . 1 1 33 33 LEU N N 15 2.269 0.009 . . . . . 26525 2 25 . 1 1 34 34 ILE N N 15 2.115 0.014 . . . . . 26525 2 26 . 1 1 35 35 SER N N 15 2.136 0.009 . . . . . 26525 2 27 . 1 1 37 37 GLU N N 15 2.028 0.008 . . . . . 26525 2 28 . 1 1 38 38 THR N N 15 1.967 0.022 . . . . . 26525 2 29 . 1 1 39 39 GLY N N 15 2.118 0.039 . . . . . 26525 2 30 . 1 1 40 40 GLU N N 15 1.996 0.005 . . . . . 26525 2 31 . 1 1 41 41 ALA N N 15 2.116 0.009 . . . . . 26525 2 32 . 1 1 43 43 TRP N N 15 2.133 0.008 . . . . . 26525 2 33 . 1 1 45 45 ARG N N 15 2.170 0.009 . . . . . 26525 2 34 . 1 1 46 46 GLY N N 15 2.261 0.009 . . . . . 26525 2 35 . 1 1 47 47 GLU N N 15 2.220 0.008 . . . . . 26525 2 36 . 1 1 48 48 LEU N N 15 2.238 0.009 . . . . . 26525 2 37 . 1 1 49 49 ASN N N 15 2.132 0.074 . . . . . 26525 2 38 . 1 1 50 50 GLY N N 15 2.130 0.008 . . . . . 26525 2 39 . 1 1 51 51 LYS N N 15 2.108 0.006 . . . . . 26525 2 40 . 1 1 53 53 GLY N N 15 2.236 0.008 . . . . . 26525 2 41 . 1 1 54 54 VAL N N 15 2.294 0.014 . . . . . 26525 2 42 . 1 1 55 55 PHE N N 15 2.044 0.011 . . . . . 26525 2 43 . 1 1 57 57 ASP N N 15 2.172 0.009 . . . . . 26525 2 44 . 1 1 58 58 ASN N N 15 2.023 0.012 . . . . . 26525 2 45 . 1 1 59 59 PHE N N 15 2.202 0.015 . . . . . 26525 2 46 . 1 1 60 60 ALA N N 15 2.215 0.008 . . . . . 26525 2 47 . 1 1 61 61 VAL N N 15 2.176 0.007 . . . . . 26525 2 48 . 1 1 63 63 ILE N N 15 2.189 0.009 . . . . . 26525 2 49 . 1 1 64 64 ASN N N 15 1.895 0.004 . . . . . 26525 2 stop_ save_ save_heteronuclear_T1_list_3 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronuclear_T1_list_3 _Heteronucl_T1_list.Entry_ID 26525 _Heteronucl_T1_list.ID 3 _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 600 _Heteronucl_T1_list.T1_coherence_type Sz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details ; Ubiquitin percent bound = 53.8 % SH3 pecent bound = 36.0 % ; _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 4 '1H-15N HSQC 4' . . . 26525 3 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 1 1 5 5 ALA N N 15 1.286 0.048 . . . . . 26525 3 2 . 1 1 6 6 LYS N N 15 1.526 0.042 . . . . . 26525 3 3 . 1 1 7 7 GLU N N 15 1.936 0.017 . . . . . 26525 3 4 . 1 1 8 8 TYR N N 15 2.017 0.025 . . . . . 26525 3 5 . 1 1 9 9 CYS N N 15 2.113 0.029 . . . . . 26525 3 6 . 1 1 10 10 ARG N N 15 2.161 0.026 . . . . . 26525 3 7 . 1 1 11 11 THR N N 15 2.187 0.019 . . . . . 26525 3 8 . 1 1 13 13 PHE N N 15 2.040 0.021 . . . . . 26525 3 9 . 1 1 15 15 TYR N N 15 2.051 0.021 . . . . . 26525 3 10 . 1 1 16 16 GLU N N 15 1.963 0.025 . . . . . 26525 3 11 . 1 1 17 17 GLY N N 15 2.036 0.020 . . . . . 26525 3 12 . 1 1 18 18 THR N N 15 2.224 0.022 . . . . . 26525 3 13 . 1 1 19 19 ASN N N 15 1.879 0.016 . . . . . 26525 3 14 . 1 1 20 20 ASN N N 15 1.983 0.044 . . . . . 26525 3 15 . 1 1 21 21 ASP N N 15 2.006 0.016 . . . . . 26525 3 16 . 1 1 23 23 LEU N N 15 2.145 0.022 . . . . . 26525 3 17 . 1 1 24 24 THR N N 15 1.841 0.018 . . . . . 26525 3 18 . 1 1 25 25 PHE N N 15 2.144 0.024 . . . . . 26525 3 19 . 1 1 26 26 LYS N N 15 1.995 0.033 . . . . . 26525 3 20 . 1 1 27 27 GLU N N 15 2.027 0.022 . . . . . 26525 3 21 . 1 1 28 28 GLY N N 15 2.167 0.024 . . . . . 26525 3 22 . 1 1 29 29 GLU N N 15 2.028 0.020 . . . . . 26525 3 23 . 1 1 31 31 ILE N N 15 2.083 0.027 . . . . . 26525 3 24 . 1 1 33 33 LEU N N 15 2.164 0.028 . . . . . 26525 3 25 . 1 1 34 34 ILE N N 15 2.012 0.033 . . . . . 26525 3 26 . 1 1 35 35 SER N N 15 2.021 0.017 . . . . . 26525 3 27 . 1 1 37 37 GLU N N 15 1.956 0.019 . . . . . 26525 3 28 . 1 1 38 38 THR N N 15 1.864 0.032 . . . . . 26525 3 29 . 1 1 39 39 GLY N N 15 2.049 0.037 . . . . . 26525 3 30 . 1 1 40 40 GLU N N 15 1.899 0.013 . . . . . 26525 3 31 . 1 1 41 41 ALA N N 15 1.959 0.027 . . . . . 26525 3 32 . 1 1 43 43 TRP N N 15 2.085 0.022 . . . . . 26525 3 33 . 1 1 45 45 ARG N N 15 2.098 0.025 . . . . . 26525 3 34 . 1 1 46 46 GLY N N 15 2.157 0.021 . . . . . 26525 3 35 . 1 1 47 47 GLU N N 15 2.104 0.019 . . . . . 26525 3 36 . 1 1 48 48 LEU N N 15 2.143 0.018 . . . . . 26525 3 37 . 1 1 49 49 ASN N N 15 2.049 0.079 . . . . . 26525 3 38 . 1 1 50 50 GLY N N 15 2.038 0.025 . . . . . 26525 3 39 . 1 1 51 51 LYS N N 15 2.036 0.022 . . . . . 26525 3 40 . 1 1 53 53 GLY N N 15 2.141 0.017 . . . . . 26525 3 41 . 1 1 54 54 VAL N N 15 2.165 0.027 . . . . . 26525 3 42 . 1 1 55 55 PHE N N 15 1.977 0.030 . . . . . 26525 3 43 . 1 1 57 57 ASP N N 15 2.071 0.025 . . . . . 26525 3 44 . 1 1 58 58 ASN N N 15 1.961 0.019 . . . . . 26525 3 45 . 1 1 59 59 PHE N N 15 2.125 0.022 . . . . . 26525 3 46 . 1 1 60 60 ALA N N 15 2.152 0.016 . . . . . 26525 3 47 . 1 1 61 61 VAL N N 15 2.073 0.019 . . . . . 26525 3 48 . 1 1 63 63 ILE N N 15 2.099 0.022 . . . . . 26525 3 49 . 1 1 64 64 ASN N N 15 1.893 0.011 . . . . . 26525 3 stop_ save_ save_heteronuclear_T1_list_4 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronuclear_T1_list_4 _Heteronucl_T1_list.Entry_ID 26525 _Heteronucl_T1_list.ID 4 _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 600 _Heteronucl_T1_list.T1_coherence_type Sz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details ; Ubiquitin percent bound = 28.4 % SH3 pecent bound = 59.3 % ; _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 5 '1H-15N HSQC 5' . . . 26525 4 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 1 1 5 5 ALA N N 15 1.297 0.036 . . . . . 26525 4 2 . 1 1 6 6 LYS N N 15 1.503 0.031 . . . . . 26525 4 3 . 1 1 7 7 GLU N N 15 1.820 0.026 . . . . . 26525 4 4 . 1 1 8 8 TYR N N 15 1.850 0.023 . . . . . 26525 4 5 . 1 1 9 9 CYS N N 15 2.001 0.032 . . . . . 26525 4 6 . 1 1 10 10 ARG N N 15 2.011 0.030 . . . . . 26525 4 7 . 1 1 11 11 THR N N 15 2.008 0.026 . . . . . 26525 4 8 . 1 1 13 13 PHE N N 15 1.842 0.021 . . . . . 26525 4 9 . 1 1 15 15 TYR N N 15 1.955 0.026 . . . . . 26525 4 10 . 1 1 16 16 GLU N N 15 1.774 0.022 . . . . . 26525 4 11 . 1 1 17 17 GLY N N 15 1.874 0.028 . . . . . 26525 4 12 . 1 1 18 18 THR N N 15 2.078 0.030 . . . . . 26525 4 13 . 1 1 19 19 ASN N N 15 1.749 0.022 . . . . . 26525 4 14 . 1 1 20 20 ASN N N 15 1.862 0.095 . . . . . 26525 4 15 . 1 1 21 21 ASP N N 15 1.855 0.021 . . . . . 26525 4 16 . 1 1 23 23 LEU N N 15 1.993 0.028 . . . . . 26525 4 17 . 1 1 24 24 THR N N 15 1.729 0.022 . . . . . 26525 4 18 . 1 1 25 25 PHE N N 15 1.953 0.021 . . . . . 26525 4 19 . 1 1 26 26 LYS N N 15 1.839 0.032 . . . . . 26525 4 20 . 1 1 27 27 GLU N N 15 1.863 0.021 . . . . . 26525 4 21 . 1 1 28 28 GLY N N 15 2.050 0.034 . . . . . 26525 4 22 . 1 1 29 29 GLU N N 15 1.878 0.023 . . . . . 26525 4 23 . 1 1 31 31 ILE N N 15 1.979 0.033 . . . . . 26525 4 24 . 1 1 33 33 LEU N N 15 2.036 0.040 . . . . . 26525 4 25 . 1 1 34 34 ILE N N 15 1.846 0.041 . . . . . 26525 4 26 . 1 1 35 35 SER N N 15 1.866 0.024 . . . . . 26525 4 27 . 1 1 37 37 GLU N N 15 1.799 0.020 . . . . . 26525 4 28 . 1 1 38 38 THR N N 15 1.664 0.030 . . . . . 26525 4 29 . 1 1 39 39 GLY N N 15 1.929 0.057 . . . . . 26525 4 30 . 1 1 40 40 GLU N N 15 1.782 0.016 . . . . . 26525 4 31 . 1 1 41 41 ALA N N 15 1.788 0.030 . . . . . 26525 4 32 . 1 1 43 43 TRP N N 15 1.965 0.027 . . . . . 26525 4 33 . 1 1 45 45 ARG N N 15 2.006 0.033 . . . . . 26525 4 34 . 1 1 46 46 GLY N N 15 2.009 0.027 . . . . . 26525 4 35 . 1 1 47 47 GLU N N 15 1.926 0.025 . . . . . 26525 4 36 . 1 1 48 48 LEU N N 15 1.986 0.025 . . . . . 26525 4 37 . 1 1 49 49 ASN N N 15 1.984 0.051 . . . . . 26525 4 38 . 1 1 50 50 GLY N N 15 1.879 0.025 . . . . . 26525 4 39 . 1 1 51 51 LYS N N 15 1.870 0.019 . . . . . 26525 4 40 . 1 1 53 53 GLY N N 15 1.925 0.023 . . . . . 26525 4 41 . 1 1 54 54 VAL N N 15 2.047 0.039 . . . . . 26525 4 42 . 1 1 55 55 PHE N N 15 1.851 0.037 . . . . . 26525 4 43 . 1 1 57 57 ASP N N 15 1.955 0.029 . . . . . 26525 4 44 . 1 1 58 58 ASN N N 15 1.853 0.020 . . . . . 26525 4 45 . 1 1 59 59 PHE N N 15 1.964 0.026 . . . . . 26525 4 46 . 1 1 60 60 ALA N N 15 2.006 0.019 . . . . . 26525 4 47 . 1 1 61 61 VAL N N 15 1.897 0.022 . . . . . 26525 4 48 . 1 1 63 63 ILE N N 15 1.959 0.029 . . . . . 26525 4 49 . 1 1 64 64 ASN N N 15 1.771 0.012 . . . . . 26525 4 stop_ save_ save_heteronuclear_T1_list_5 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronuclear_T1_list_5 _Heteronucl_T1_list.Entry_ID 26525 _Heteronucl_T1_list.ID 5 _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 600 _Heteronucl_T1_list.T1_coherence_type Sz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details . _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 2 '1H-15N HSQC 2' . . . 26525 5 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 2 2 8 8 GLN N N 15 2.010 0.018 . 2 2 GLN N 26525 5 2 . 2 2 10 10 PHE N N 15 2.244 0.022 . 2 4 PHE N 26525 5 3 . 2 2 11 11 VAL N N 15 2.086 0.021 . 2 5 VAL N 26525 5 4 . 2 2 12 12 LYS N N 15 2.238 0.029 . 2 6 LYS N 26525 5 5 . 2 2 13 13 THR N N 15 2.090 0.018 . 2 7 THR N 26525 5 6 . 2 2 14 14 LEU N N 15 2.168 0.063 . 2 8 LEU N 26525 5 7 . 2 2 15 15 THR N N 15 1.991 0.087 . 2 9 THR N 26525 5 8 . 2 2 16 16 GLY N N 15 2.054 0.030 . 2 10 GLY N 26525 5 9 . 2 2 17 17 LYS N N 15 1.875 0.029 . 2 11 LYS N 26525 5 10 . 2 2 18 18 THR N N 15 2.098 0.026 . 2 12 THR N 26525 5 11 . 2 2 19 19 ILE N N 15 2.125 0.023 . 2 13 ILE N 26525 5 12 . 2 2 20 20 THR N N 15 2.109 0.019 . 2 14 THR N 26525 5 13 . 2 2 21 21 LEU N N 15 2.179 0.018 . 2 15 LEU N 26525 5 14 . 2 2 22 22 GLU N N 15 1.915 0.015 . 2 16 GLU N 26525 5 15 . 2 2 23 23 VAL N N 15 2.117 0.025 . 2 17 VAL N 26525 5 16 . 2 2 24 24 GLU N N 15 1.959 0.016 . 2 18 GLU N 26525 5 17 . 2 2 26 26 SER N N 15 2.012 0.025 . 2 20 SER N 26525 5 18 . 2 2 28 28 THR N N 15 2.195 0.025 . 2 22 THR N 26525 5 19 . 2 2 31 31 ASN N N 15 2.211 0.019 . 2 25 ASN N 26525 5 20 . 2 2 32 32 VAL N N 15 2.239 0.014 . 2 26 VAL N 26525 5 21 . 2 2 33 33 LYS N N 15 2.314 0.025 . 2 27 LYS N 26525 5 22 . 2 2 34 34 ALA N N 15 2.274 0.014 . 2 28 ALA N 26525 5 23 . 2 2 35 35 LYS N N 15 2.256 0.023 . 2 29 LYS N 26525 5 24 . 2 2 36 36 ILE N N 15 2.247 0.019 . 2 30 ILE N 26525 5 25 . 2 2 37 37 GLN N N 15 2.280 0.017 . 2 31 GLN N 26525 5 26 . 2 2 38 38 ASP N N 15 2.201 0.016 . 2 32 ASP N 26525 5 27 . 2 2 39 39 LYS N N 15 2.087 0.020 . 2 33 LYS N 26525 5 28 . 2 2 40 40 GLU N N 15 2.116 0.022 . 2 34 GLU N 26525 5 29 . 2 2 41 41 GLY N N 15 2.074 0.035 . 2 35 GLY N 26525 5 30 . 2 2 42 42 ILE N N 15 1.682 0.024 . 2 36 ILE N 26525 5 31 . 2 2 45 45 ASP N N 15 2.232 0.017 . 2 39 ASP N 26525 5 32 . 2 2 46 46 GLN N N 15 2.144 0.021 . 2 40 GLN N 26525 5 33 . 2 2 47 47 GLN N N 15 2.165 0.021 . 2 41 GLN N 26525 5 34 . 2 2 48 48 ARG N N 15 2.215 0.030 . 2 42 ARG N 26525 5 35 . 2 2 49 49 LEU N N 15 2.127 0.023 . 2 43 LEU N 26525 5 36 . 2 2 50 50 ILE N N 15 2.181 0.019 . 2 44 ILE N 26525 5 37 . 2 2 51 51 PHE N N 15 2.188 0.020 . 2 45 PHE N 26525 5 38 . 2 2 52 52 ALA N N 15 2.204 0.072 . 2 46 ALA N 26525 5 39 . 2 2 53 53 GLY N N 15 2.186 0.025 . 2 47 GLY N 26525 5 40 . 2 2 54 54 LYS N N 15 2.005 0.025 . 2 48 LYS N 26525 5 41 . 2 2 55 55 GLN N N 15 2.028 0.014 . 2 49 GLN N 26525 5 42 . 2 2 57 57 GLU N N 15 1.976 0.021 . 2 51 GLU N 26525 5 43 . 2 2 58 58 ASP N N 15 1.865 0.013 . 2 52 ASP N 26525 5 44 . 2 2 59 59 GLY N N 15 2.226 0.018 . 2 53 GLY N 26525 5 45 . 2 2 60 60 ARG N N 15 1.970 0.035 . 2 54 ARG N 26525 5 46 . 2 2 61 61 THR N N 15 2.175 0.023 . 2 55 THR N 26525 5 47 . 2 2 62 62 LEU N N 15 2.285 0.019 . 2 56 LEU N 26525 5 48 . 2 2 63 63 SER N N 15 2.189 0.017 . 2 57 SER N 26525 5 49 . 2 2 65 65 TYR N N 15 2.113 0.021 . 2 59 TYR N 26525 5 50 . 2 2 66 66 ASN N N 15 2.242 0.023 . 2 60 ASN N 26525 5 51 . 2 2 67 67 ILE N N 15 2.167 0.024 . 2 61 ILE N 26525 5 52 . 2 2 68 68 GLN N N 15 1.846 0.017 . 2 62 GLN N 26525 5 53 . 2 2 69 69 LYS N N 15 2.043 0.018 . 2 63 LYS N 26525 5 54 . 2 2 70 70 GLU N N 15 2.208 0.023 . 2 64 GLU N 26525 5 55 . 2 2 71 71 SER N N 15 2.119 0.020 . 2 65 SER N 26525 5 56 . 2 2 72 72 THR N N 15 2.132 0.021 . 2 66 THR N 26525 5 57 . 2 2 74 74 HIS N N 15 2.165 0.025 . 2 68 HIS N 26525 5 58 . 2 2 75 75 LEU N N 15 2.202 0.021 . 2 69 LEU N 26525 5 59 . 2 2 76 76 VAL N N 15 2.193 0.023 . 2 70 VAL N 26525 5 60 . 2 2 77 77 LEU N N 15 2.093 0.016 . 2 71 LEU N 26525 5 61 . 2 2 78 78 ARG N N 15 2.067 0.029 . 2 72 ARG N 26525 5 62 . 2 2 79 79 LEU N N 15 1.960 0.028 . 2 73 LEU N 26525 5 63 . 2 2 80 80 ARG N N 15 1.714 0.072 . 2 74 ARG N 26525 5 64 . 2 2 81 81 GLY N N 15 1.472 0.051 . 2 75 GLY N 26525 5 65 . 2 2 82 82 GLY N N 15 1.302 0.065 . 2 76 GLY N 26525 5 stop_ save_ save_heteronuclear_T1_list_6 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronuclear_T1_list_6 _Heteronucl_T1_list.Entry_ID 26525 _Heteronucl_T1_list.ID 6 _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 600 _Heteronucl_T1_list.T1_coherence_type Sz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details ; Ubiquitin percent bound = 69.5 % SH3 pecent bound = 21.7 % ; _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 3 '1H-15N HSQC 3' . . . 26525 6 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 2 2 8 8 GLN N N 15 1.799 0.029 . 2 2 GLN N 26525 6 2 . 2 2 10 10 PHE N N 15 1.892 0.034 . 2 4 PHE N 26525 6 3 . 2 2 11 11 VAL N N 15 1.819 0.031 . 2 5 VAL N 26525 6 4 . 2 2 12 12 LYS N N 15 1.887 0.055 . 2 6 LYS N 26525 6 5 . 2 2 13 13 THR N N 15 1.861 0.032 . 2 7 THR N 26525 6 6 . 2 2 14 14 LEU N N 15 1.936 0.054 . 2 8 LEU N 26525 6 7 . 2 2 15 15 THR N N 15 1.638 0.181 . 2 9 THR N 26525 6 8 . 2 2 16 16 GLY N N 15 1.860 0.044 . 2 10 GLY N 26525 6 9 . 2 2 17 17 LYS N N 15 1.714 0.043 . 2 11 LYS N 26525 6 10 . 2 2 18 18 THR N N 15 1.791 0.040 . 2 12 THR N 26525 6 11 . 2 2 19 19 ILE N N 15 1.789 0.040 . 2 13 ILE N 26525 6 12 . 2 2 20 20 THR N N 15 1.774 0.029 . 2 14 THR N 26525 6 13 . 2 2 21 21 LEU N N 15 1.888 0.032 . 2 15 LEU N 26525 6 14 . 2 2 22 22 GLU N N 15 1.697 0.019 . 2 16 GLU N 26525 6 15 . 2 2 23 23 VAL N N 15 1.842 0.025 . 2 17 VAL N 26525 6 16 . 2 2 24 24 GLU N N 15 1.749 0.026 . 2 18 GLU N 26525 6 17 . 2 2 26 26 SER N N 15 1.699 0.025 . 2 20 SER N 26525 6 18 . 2 2 28 28 THR N N 15 1.783 0.030 . 2 22 THR N 26525 6 19 . 2 2 31 31 ASN N N 15 1.929 0.032 . 2 25 ASN N 26525 6 20 . 2 2 32 32 VAL N N 15 1.945 0.021 . 2 26 VAL N 26525 6 21 . 2 2 33 33 LYS N N 15 1.986 0.038 . 2 27 LYS N 26525 6 22 . 2 2 34 34 ALA N N 15 1.933 0.021 . 2 28 ALA N 26525 6 23 . 2 2 35 35 LYS N N 15 1.925 0.032 . 2 29 LYS N 26525 6 24 . 2 2 36 36 ILE N N 15 1.891 0.033 . 2 30 ILE N 26525 6 25 . 2 2 37 37 GLN N N 15 1.945 0.026 . 2 31 GLN N 26525 6 26 . 2 2 38 38 ASP N N 15 1.839 0.021 . 2 32 ASP N 26525 6 27 . 2 2 39 39 LYS N N 15 1.777 0.031 . 2 33 LYS N 26525 6 28 . 2 2 40 40 GLU N N 15 1.822 0.041 . 2 34 GLU N 26525 6 29 . 2 2 41 41 GLY N N 15 1.775 0.038 . 2 35 GLY N 26525 6 30 . 2 2 42 42 ILE N N 15 1.457 0.035 . 2 36 ILE N 26525 6 31 . 2 2 45 45 ASP N N 15 1.915 0.024 . 2 39 ASP N 26525 6 32 . 2 2 46 46 GLN N N 15 1.730 0.030 . 2 40 GLN N 26525 6 33 . 2 2 47 47 GLN N N 15 1.758 0.033 . 2 41 GLN N 26525 6 34 . 2 2 48 48 ARG N N 15 1.802 0.067 . 2 42 ARG N 26525 6 35 . 2 2 49 49 LEU N N 15 1.779 0.031 . 2 43 LEU N 26525 6 36 . 2 2 50 50 ILE N N 15 1.826 0.037 . 2 44 ILE N 26525 6 37 . 2 2 51 51 PHE N N 15 1.812 0.042 . 2 45 PHE N 26525 6 38 . 2 2 52 52 ALA N N 15 1.803 0.102 . 2 46 ALA N 26525 6 39 . 2 2 53 53 GLY N N 15 1.808 0.048 . 2 47 GLY N 26525 6 40 . 2 2 54 54 LYS N N 15 1.707 0.033 . 2 48 LYS N 26525 6 41 . 2 2 55 55 GLN N N 15 1.702 0.023 . 2 49 GLN N 26525 6 42 . 2 2 57 57 GLU N N 15 1.689 0.032 . 2 51 GLU N 26525 6 43 . 2 2 58 58 ASP N N 15 1.551 0.017 . 2 52 ASP N 26525 6 44 . 2 2 59 59 GLY N N 15 1.850 0.030 . 2 53 GLY N 26525 6 45 . 2 2 60 60 ARG N N 15 1.680 0.036 . 2 54 ARG N 26525 6 46 . 2 2 61 61 THR N N 15 1.817 0.037 . 2 55 THR N 26525 6 47 . 2 2 62 62 LEU N N 15 1.902 0.028 . 2 56 LEU N 26525 6 48 . 2 2 63 63 SER N N 15 1.855 0.024 . 2 57 SER N 26525 6 49 . 2 2 65 65 TYR N N 15 1.781 0.027 . 2 59 TYR N 26525 6 50 . 2 2 66 66 ASN N N 15 1.825 0.031 . 2 60 ASN N 26525 6 51 . 2 2 67 67 ILE N N 15 1.876 0.029 . 2 61 ILE N 26525 6 52 . 2 2 68 68 GLN N N 15 1.522 0.022 . 2 62 GLN N 26525 6 53 . 2 2 69 69 LYS N N 15 1.731 0.026 . 2 63 LYS N 26525 6 54 . 2 2 70 70 GLU N N 15 1.894 0.040 . 2 64 GLU N 26525 6 55 . 2 2 71 71 SER N N 15 1.860 0.034 . 2 65 SER N 26525 6 56 . 2 2 72 72 THR N N 15 1.737 0.028 . 2 66 THR N 26525 6 57 . 2 2 74 74 HIS N N 15 1.815 0.044 . 2 68 HIS N 26525 6 58 . 2 2 75 75 LEU N N 15 1.886 0.032 . 2 69 LEU N 26525 6 59 . 2 2 76 76 VAL N N 15 1.840 0.040 . 2 70 VAL N 26525 6 60 . 2 2 77 77 LEU N N 15 1.782 0.031 . 2 71 LEU N 26525 6 61 . 2 2 78 78 ARG N N 15 1.865 0.045 . 2 72 ARG N 26525 6 62 . 2 2 79 79 LEU N N 15 1.772 0.076 . 2 73 LEU N 26525 6 63 . 2 2 80 80 ARG N N 15 1.721 0.056 . 2 74 ARG N 26525 6 64 . 2 2 81 81 GLY N N 15 1.486 0.075 . 2 75 GLY N 26525 6 65 . 2 2 82 82 GLY N N 15 1.486 0.054 . 2 76 GLY N 26525 6 stop_ save_ save_heteronuclear_T1_list_7 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronuclear_T1_list_7 _Heteronucl_T1_list.Entry_ID 26525 _Heteronucl_T1_list.ID 7 _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 600 _Heteronucl_T1_list.T1_coherence_type Sz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details ; Ubiquitin percent bound = 53.8 % SH3 pecent bound = 36.0 % ; _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 4 '1H-15N HSQC 4' . . . 26525 7 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 2 2 8 8 GLN N N 15 1.815 0.027 . 2 2 GLN N 26525 7 2 . 2 2 10 10 PHE N N 15 2.010 0.029 . 2 4 PHE N 26525 7 3 . 2 2 11 11 VAL N N 15 1.888 0.043 . 2 5 VAL N 26525 7 4 . 2 2 12 12 LYS N N 15 1.945 0.039 . 2 6 LYS N 26525 7 5 . 2 2 13 13 THR N N 15 1.935 0.025 . 2 7 THR N 26525 7 6 . 2 2 14 14 LEU N N 15 1.940 0.038 . 2 8 LEU N 26525 7 7 . 2 2 15 15 THR N N 15 1.743 0.109 . 2 9 THR N 26525 7 8 . 2 2 16 16 GLY N N 15 1.901 0.036 . 2 10 GLY N 26525 7 9 . 2 2 17 17 LYS N N 15 1.756 0.026 . 2 11 LYS N 26525 7 10 . 2 2 18 18 THR N N 15 1.935 0.045 . 2 12 THR N 26525 7 11 . 2 2 19 19 ILE N N 15 1.864 0.030 . 2 13 ILE N 26525 7 12 . 2 2 20 20 THR N N 15 1.855 0.024 . 2 14 THR N 26525 7 13 . 2 2 21 21 LEU N N 15 1.919 0.030 . 2 15 LEU N 26525 7 14 . 2 2 22 22 GLU N N 15 1.749 0.018 . 2 16 GLU N 26525 7 15 . 2 2 23 23 VAL N N 15 1.890 0.023 . 2 17 VAL N 26525 7 16 . 2 2 24 24 GLU N N 15 1.779 0.023 . 2 18 GLU N 26525 7 17 . 2 2 26 26 SER N N 15 1.734 0.021 . 2 20 SER N 26525 7 18 . 2 2 28 28 THR N N 15 1.948 0.028 . 2 22 THR N 26525 7 19 . 2 2 31 31 ASN N N 15 1.991 0.039 . 2 25 ASN N 26525 7 20 . 2 2 32 32 VAL N N 15 1.974 0.034 . 2 26 VAL N 26525 7 21 . 2 2 33 33 LYS N N 15 2.049 0.034 . 2 27 LYS N 26525 7 22 . 2 2 34 34 ALA N N 15 1.986 0.017 . 2 28 ALA N 26525 7 23 . 2 2 35 35 LYS N N 15 1.996 0.027 . 2 29 LYS N 26525 7 24 . 2 2 36 36 ILE N N 15 1.985 0.028 . 2 30 ILE N 26525 7 25 . 2 2 37 37 GLN N N 15 1.999 0.026 . 2 31 GLN N 26525 7 26 . 2 2 38 38 ASP N N 15 1.936 0.018 . 2 32 ASP N 26525 7 27 . 2 2 39 39 LYS N N 15 1.830 0.029 . 2 33 LYS N 26525 7 28 . 2 2 40 40 GLU N N 15 1.853 0.029 . 2 34 GLU N 26525 7 29 . 2 2 41 41 GLY N N 15 1.822 0.034 . 2 35 GLY N 26525 7 30 . 2 2 42 42 ILE N N 15 1.509 0.020 . 2 36 ILE N 26525 7 31 . 2 2 45 45 ASP N N 15 1.934 0.018 . 2 39 ASP N 26525 7 32 . 2 2 46 46 GLN N N 15 1.864 0.025 . 2 40 GLN N 26525 7 33 . 2 2 47 47 GLN N N 15 1.868 0.025 . 2 41 GLN N 26525 7 34 . 2 2 48 48 ARG N N 15 1.869 0.047 . 2 42 ARG N 26525 7 35 . 2 2 49 49 LEU N N 15 1.892 0.029 . 2 43 LEU N 26525 7 36 . 2 2 50 50 ILE N N 15 1.934 0.036 . 2 44 ILE N 26525 7 37 . 2 2 51 51 PHE N N 15 1.864 0.034 . 2 45 PHE N 26525 7 38 . 2 2 52 52 ALA N N 15 1.957 0.085 . 2 46 ALA N 26525 7 39 . 2 2 53 53 GLY N N 15 1.847 0.034 . 2 47 GLY N 26525 7 40 . 2 2 54 54 LYS N N 15 1.822 0.037 . 2 48 LYS N 26525 7 41 . 2 2 55 55 GLN N N 15 1.821 0.021 . 2 49 GLN N 26525 7 42 . 2 2 57 57 GLU N N 15 1.796 0.029 . 2 51 GLU N 26525 7 43 . 2 2 58 58 ASP N N 15 1.667 0.018 . 2 52 ASP N 26525 7 44 . 2 2 59 59 GLY N N 15 1.974 0.030 . 2 53 GLY N 26525 7 45 . 2 2 60 60 ARG N N 15 1.720 0.045 . 2 54 ARG N 26525 7 46 . 2 2 61 61 THR N N 15 1.911 0.030 . 2 55 THR N 26525 7 47 . 2 2 62 62 LEU N N 15 2.013 0.032 . 2 56 LEU N 26525 7 48 . 2 2 63 63 SER N N 15 1.922 0.029 . 2 57 SER N 26525 7 49 . 2 2 65 65 TYR N N 15 1.891 0.026 . 2 59 TYR N 26525 7 50 . 2 2 66 66 ASN N N 15 1.913 0.031 . 2 60 ASN N 26525 7 51 . 2 2 67 67 ILE N N 15 1.925 0.029 . 2 61 ILE N 26525 7 52 . 2 2 68 68 GLN N N 15 1.637 0.026 . 2 62 GLN N 26525 7 53 . 2 2 69 69 LYS N N 15 1.779 0.022 . 2 63 LYS N 26525 7 54 . 2 2 70 70 GLU N N 15 2.015 0.047 . 2 64 GLU N 26525 7 55 . 2 2 71 71 SER N N 15 1.860 0.021 . 2 65 SER N 26525 7 56 . 2 2 72 72 THR N N 15 1.846 0.028 . 2 66 THR N 26525 7 57 . 2 2 74 74 HIS N N 15 1.920 0.040 . 2 68 HIS N 26525 7 58 . 2 2 75 75 LEU N N 15 1.926 0.029 . 2 69 LEU N 26525 7 59 . 2 2 76 76 VAL N N 15 1.929 0.043 . 2 70 VAL N 26525 7 60 . 2 2 77 77 LEU N N 15 1.847 0.022 . 2 71 LEU N 26525 7 61 . 2 2 78 78 ARG N N 15 1.916 0.039 . 2 72 ARG N 26525 7 62 . 2 2 79 79 LEU N N 15 1.753 0.068 . 2 73 LEU N 26525 7 63 . 2 2 80 80 ARG N N 15 1.703 0.055 . 2 74 ARG N 26525 7 64 . 2 2 81 81 GLY N N 15 1.600 0.074 . 2 75 GLY N 26525 7 65 . 2 2 82 82 GLY N N 15 1.420 0.054 . 2 76 GLY N 26525 7 stop_ save_ save_heteronuclear_T1_list_8 _Heteronucl_T1_list.Sf_category heteronucl_T1_relaxation _Heteronucl_T1_list.Sf_framecode heteronuclear_T1_list_8 _Heteronucl_T1_list.Entry_ID 26525 _Heteronucl_T1_list.ID 8 _Heteronucl_T1_list.Sample_condition_list_ID 1 _Heteronucl_T1_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T1_list.Spectrometer_frequency_1H 600 _Heteronucl_T1_list.T1_coherence_type Sz _Heteronucl_T1_list.T1_val_units s-1 _Heteronucl_T1_list.Details ; Ubiquitin percent bound = 28.4 % SH3 pecent bound = 59.3 % ; _Heteronucl_T1_list.Text_data_format . _Heteronucl_T1_list.Text_data . loop_ _Heteronucl_T1_experiment.Experiment_ID _Heteronucl_T1_experiment.Experiment_name _Heteronucl_T1_experiment.Sample_ID _Heteronucl_T1_experiment.Sample_label _Heteronucl_T1_experiment.Sample_state _Heteronucl_T1_experiment.Entry_ID _Heteronucl_T1_experiment.Heteronucl_T1_list_ID 5 '1H-15N HSQC 5' . . . 26525 8 stop_ loop_ _T1.ID _T1.Assembly_atom_ID _T1.Entity_assembly_ID _T1.Entity_ID _T1.Comp_index_ID _T1.Seq_ID _T1.Comp_ID _T1.Atom_ID _T1.Atom_type _T1.Atom_isotope_number _T1.Val _T1.Val_err _T1.Resonance_ID _T1.Auth_entity_assembly_ID _T1.Auth_seq_ID _T1.Auth_comp_ID _T1.Auth_atom_ID _T1.Entry_ID _T1.Heteronucl_T1_list_ID 1 . 2 2 8 8 GLN N N 15 1.916 0.007 . 2 2 GLN N 26525 8 2 . 2 2 10 10 PHE N N 15 2.085 0.011 . 2 4 PHE N 26525 8 3 . 2 2 11 11 VAL N N 15 1.947 0.009 . 2 5 VAL N 26525 8 4 . 2 2 12 12 LYS N N 15 2.057 0.014 . 2 6 LYS N 26525 8 5 . 2 2 13 13 THR N N 15 2.025 0.008 . 2 7 THR N 26525 8 6 . 2 2 14 14 LEU N N 15 2.077 0.042 . 2 8 LEU N 26525 8 7 . 2 2 15 15 THR N N 15 1.810 0.078 . 2 9 THR N 26525 8 8 . 2 2 16 16 GLY N N 15 1.987 0.022 . 2 10 GLY N 26525 8 9 . 2 2 17 17 LYS N N 15 1.809 0.019 . 2 11 LYS N 26525 8 10 . 2 2 18 18 THR N N 15 1.969 0.028 . 2 12 THR N 26525 8 11 . 2 2 19 19 ILE N N 15 1.991 0.011 . 2 13 ILE N 26525 8 12 . 2 2 20 20 THR N N 15 1.949 0.009 . 2 14 THR N 26525 8 13 . 2 2 21 21 LEU N N 15 2.053 0.011 . 2 15 LEU N 26525 8 14 . 2 2 22 22 GLU N N 15 1.847 0.009 . 2 16 GLU N 26525 8 15 . 2 2 23 23 VAL N N 15 2.024 0.009 . 2 17 VAL N 26525 8 16 . 2 2 24 24 GLU N N 15 1.842 0.007 . 2 18 GLU N 26525 8 17 . 2 2 26 26 SER N N 15 1.898 0.008 . 2 20 SER N 26525 8 18 . 2 2 28 28 THR N N 15 2.028 0.010 . 2 22 THR N 26525 8 19 . 2 2 31 31 ASN N N 15 2.095 0.008 . 2 25 ASN N 26525 8 20 . 2 2 32 32 VAL N N 15 2.107 0.006 . 2 26 VAL N 26525 8 21 . 2 2 33 33 LYS N N 15 2.175 0.012 . 2 27 LYS N 26525 8 22 . 2 2 34 34 ALA N N 15 2.104 0.006 . 2 28 ALA N 26525 8 23 . 2 2 35 35 LYS N N 15 2.069 0.011 . 2 29 LYS N 26525 8 24 . 2 2 36 36 ILE N N 15 2.092 0.008 . 2 30 ILE N 26525 8 25 . 2 2 37 37 GLN N N 15 2.152 0.008 . 2 31 GLN N 26525 8 26 . 2 2 38 38 ASP N N 15 2.099 0.006 . 2 32 ASP N 26525 8 27 . 2 2 39 39 LYS N N 15 1.978 0.014 . 2 33 LYS N 26525 8 28 . 2 2 40 40 GLU N N 15 1.995 0.009 . 2 34 GLU N 26525 8 29 . 2 2 41 41 GLY N N 15 1.934 0.014 . 2 35 GLY N 26525 8 30 . 2 2 42 42 ILE N N 15 1.557 0.008 . 2 36 ILE N 26525 8 31 . 2 2 45 45 ASP N N 15 2.077 0.006 . 2 39 ASP N 26525 8 32 . 2 2 46 46 GLN N N 15 1.993 0.010 . 2 40 GLN N 26525 8 33 . 2 2 47 47 GLN N N 15 1.972 0.010 . 2 41 GLN N 26525 8 34 . 2 2 48 48 ARG N N 15 2.031 0.015 . 2 42 ARG N 26525 8 35 . 2 2 49 49 LEU N N 15 2.006 0.009 . 2 43 LEU N 26525 8 36 . 2 2 50 50 ILE N N 15 2.071 0.009 . 2 44 ILE N 26525 8 37 . 2 2 51 51 PHE N N 15 2.059 0.010 . 2 45 PHE N 26525 8 38 . 2 2 52 52 ALA N N 15 2.094 0.065 . 2 46 ALA N 26525 8 39 . 2 2 53 53 GLY N N 15 2.030 0.011 . 2 47 GLY N 26525 8 40 . 2 2 54 54 LYS N N 15 1.882 0.008 . 2 48 LYS N 26525 8 41 . 2 2 55 55 GLN N N 15 1.918 0.008 . 2 49 GLN N 26525 8 42 . 2 2 57 57 GLU N N 15 1.886 0.008 . 2 51 GLU N 26525 8 43 . 2 2 58 58 ASP N N 15 1.753 0.005 . 2 52 ASP N 26525 8 44 . 2 2 59 59 GLY N N 15 2.037 0.009 . 2 53 GLY N 26525 8 45 . 2 2 60 60 ARG N N 15 1.824 0.011 . 2 54 ARG N 26525 8 46 . 2 2 61 61 THR N N 15 2.013 0.010 . 2 55 THR N 26525 8 47 . 2 2 62 62 LEU N N 15 2.140 0.008 . 2 56 LEU N 26525 8 48 . 2 2 63 63 SER N N 15 2.015 0.011 . 2 57 SER N 26525 8 49 . 2 2 65 65 TYR N N 15 1.974 0.010 . 2 59 TYR N 26525 8 50 . 2 2 66 66 ASN N N 15 2.069 0.009 . 2 60 ASN N 26525 8 51 . 2 2 67 67 ILE N N 15 2.038 0.011 . 2 61 ILE N 26525 8 52 . 2 2 68 68 GLN N N 15 1.741 0.007 . 2 62 GLN N 26525 8 53 . 2 2 69 69 LYS N N 15 1.963 0.008 . 2 63 LYS N 26525 8 54 . 2 2 70 70 GLU N N 15 2.116 0.011 . 2 64 GLU N 26525 8 55 . 2 2 71 71 SER N N 15 2.005 0.007 . 2 65 SER N 26525 8 56 . 2 2 72 72 THR N N 15 1.956 0.009 . 2 66 THR N 26525 8 57 . 2 2 74 74 HIS N N 15 2.016 0.011 . 2 68 HIS N 26525 8 58 . 2 2 75 75 LEU N N 15 2.041 0.010 . 2 69 LEU N 26525 8 59 . 2 2 76 76 VAL N N 15 2.052 0.011 . 2 70 VAL N 26525 8 60 . 2 2 77 77 LEU N N 15 1.988 0.007 . 2 71 LEU N 26525 8 61 . 2 2 78 78 ARG N N 15 1.989 0.011 . 2 72 ARG N 26525 8 62 . 2 2 79 79 LEU N N 15 1.835 0.023 . 2 73 LEU N 26525 8 63 . 2 2 80 80 ARG N N 15 1.622 0.051 . 2 74 ARG N 26525 8 64 . 2 2 81 81 GLY N N 15 1.456 0.048 . 2 75 GLY N 26525 8 65 . 2 2 82 82 GLY N N 15 1.359 0.048 . 2 76 GLY N 26525 8 stop_ save_ ######################################## # Heteronuclear T2 relaxation values # ######################################## save_heteronuclear_T2_list_1 _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronuclear_T2_list_1 _Heteronucl_T2_list.Entry_ID 26525 _Heteronucl_T2_list.ID 1 _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method 'no calibration applied' _Heteronucl_T2_list.Temp_control_method 'no temperature control applied' _Heteronucl_T2_list.Spectrometer_frequency_1H 600 _Heteronucl_T2_list.T2_coherence_type S(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details . _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 1 '1H-15N HSQC 1' . . . 26525 1 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 1 1 5 5 ALA N N 15 2.579 0.055 . . . . . . . 26525 1 2 . 1 1 6 6 LYS N N 15 3.307 0.072 . . . . . . . 26525 1 3 . 1 1 7 7 GLU N N 15 4.263 0.074 . . . . . . . 26525 1 4 . 1 1 8 8 TYR N N 15 4.818 0.053 . . . . . . . 26525 1 5 . 1 1 9 9 CYS N N 15 5.017 0.069 . . . . . . . 26525 1 6 . 1 1 10 10 ARG N N 15 4.957 0.060 . . . . . . . 26525 1 7 . 1 1 11 11 THR N N 15 5.086 0.051 . . . . . . . 26525 1 8 . 1 1 13 13 PHE N N 15 5.104 0.055 . . . . . . . 26525 1 9 . 1 1 15 15 TYR N N 15 5.037 0.050 . . . . . . . 26525 1 10 . 1 1 16 16 GLU N N 15 4.865 0.059 . . . . . . . 26525 1 11 . 1 1 17 17 GLY N N 15 4.973 0.087 . . . . . . . 26525 1 12 . 1 1 18 18 THR N N 15 5.687 0.052 . . . . . . . 26525 1 13 . 1 1 19 19 ASN N N 15 4.627 0.035 . . . . . . . 26525 1 14 . 1 1 20 20 ASN N N 15 4.898 0.080 . . . . . . . 26525 1 15 . 1 1 21 21 ASP N N 15 5.025 0.057 . . . . . . . 26525 1 16 . 1 1 23 23 LEU N N 15 4.750 0.078 . . . . . . . 26525 1 17 . 1 1 24 24 THR N N 15 4.115 0.051 . . . . . . . 26525 1 18 . 1 1 25 25 PHE N N 15 5.367 0.065 . . . . . . . 26525 1 19 . 1 1 26 26 LYS N N 15 4.971 0.082 . . . . . . . 26525 1 20 . 1 1 27 27 GLU N N 15 5.103 0.042 . . . . . . . 26525 1 21 . 1 1 28 28 GLY N N 15 4.856 0.070 . . . . . . . 26525 1 22 . 1 1 29 29 GLU N N 15 5.052 0.054 . . . . . . . 26525 1 23 . 1 1 31 31 ILE N N 15 4.835 0.180 . . . . . . . 26525 1 24 . 1 1 33 33 LEU N N 15 5.552 0.126 . . . . . . . 26525 1 25 . 1 1 34 34 ILE N N 15 5.102 0.213 . . . . . . . 26525 1 26 . 1 1 35 35 SER N N 15 5.434 0.175 . . . . . . . 26525 1 27 . 1 1 37 37 GLU N N 15 4.657 0.079 . . . . . . . 26525 1 28 . 1 1 38 38 THR N N 15 4.221 0.067 . . . . . . . 26525 1 29 . 1 1 39 39 GLY N N 15 4.301 0.182 . . . . . . . 26525 1 30 . 1 1 40 40 GLU N N 15 4.644 0.045 . . . . . . . 26525 1 31 . 1 1 41 41 ALA N N 15 4.685 0.062 . . . . . . . 26525 1 32 . 1 1 43 43 TRP N N 15 4.973 0.054 . . . . . . . 26525 1 33 . 1 1 45 45 ARG N N 15 5.308 0.064 . . . . . . . 26525 1 34 . 1 1 46 46 GLY N N 15 5.360 0.063 . . . . . . . 26525 1 35 . 1 1 47 47 GLU N N 15 5.138 0.057 . . . . . . . 26525 1 36 . 1 1 48 48 LEU N N 15 4.910 0.118 . . . . . . . 26525 1 37 . 1 1 49 49 ASN N N 15 5.441 0.283 . . . . . . . 26525 1 38 . 1 1 50 50 GLY N N 15 5.046 0.079 . . . . . . . 26525 1 39 . 1 1 51 51 LYS N N 15 4.713 0.059 . . . . . . . 26525 1 40 . 1 1 53 53 GLY N N 15 4.870 0.103 . . . . . . . 26525 1 41 . 1 1 54 54 VAL N N 15 5.200 0.258 . . . . . . . 26525 1 42 . 1 1 55 55 PHE N N 15 4.696 0.099 . . . . . . . 26525 1 43 . 1 1 57 57 ASP N N 15 5.331 0.048 . . . . . . . 26525 1 44 . 1 1 58 58 ASN N N 15 4.628 0.041 . . . . . . . 26525 1 45 . 1 1 59 59 PHE N N 15 5.041 0.068 . . . . . . . 26525 1 46 . 1 1 60 60 ALA N N 15 4.981 0.072 . . . . . . . 26525 1 47 . 1 1 61 61 VAL N N 15 4.776 0.086 . . . . . . . 26525 1 48 . 1 1 63 63 ILE N N 15 4.753 0.078 . . . . . . . 26525 1 49 . 1 1 64 64 ASN N N 15 3.831 0.038 . . . . . . . 26525 1 stop_ save_ save_heteronuclear_T2_list_2 _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronuclear_T2_list_2 _Heteronucl_T2_list.Entry_ID 26525 _Heteronucl_T2_list.ID 2 _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method 'no calibration applied' _Heteronucl_T2_list.Temp_control_method 'no temperature control applied' _Heteronucl_T2_list.Spectrometer_frequency_1H 600 _Heteronucl_T2_list.T2_coherence_type S(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details ; Ubiquitin percent bound = 69.5 % SH3 pecent bound = 21.7 % ; _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 3 '1H-15N HSQC 3' . . . 26525 2 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 1 1 5 5 ALA N N 15 2.815 0.052 . . . . . . . 26525 2 2 . 1 1 6 6 LYS N N 15 3.476 0.032 . . . . . . . 26525 2 3 . 1 1 7 7 GLU N N 15 4.855 0.021 . . . . . . . 26525 2 4 . 1 1 8 8 TYR N N 15 5.493 0.031 . . . . . . . 26525 2 5 . 1 1 9 9 CYS N N 15 5.640 0.026 . . . . . . . 26525 2 6 . 1 1 10 10 ARG N N 15 5.643 0.025 . . . . . . . 26525 2 7 . 1 1 11 11 THR N N 15 6.029 0.035 . . . . . . . 26525 2 8 . 1 1 13 13 PHE N N 15 5.909 0.040 . . . . . . . 26525 2 9 . 1 1 15 15 TYR N N 15 6.433 0.031 . . . . . . . 26525 2 10 . 1 1 16 16 GLU N N 15 5.656 0.017 . . . . . . . 26525 2 11 . 1 1 17 17 GLY N N 15 5.708 0.025 . . . . . . . 26525 2 12 . 1 1 18 18 THR N N 15 6.432 0.027 . . . . . . . 26525 2 13 . 1 1 19 19 ASN N N 15 5.260 0.020 . . . . . . . 26525 2 14 . 1 1 20 20 ASN N N 15 7.170 0.086 . . . . . . . 26525 2 15 . 1 1 21 21 ASP N N 15 6.273 0.021 . . . . . . . 26525 2 16 . 1 1 23 23 LEU N N 15 5.621 0.024 . . . . . . . 26525 2 17 . 1 1 24 24 THR N N 15 5.059 0.015 . . . . . . . 26525 2 18 . 1 1 25 25 PHE N N 15 6.296 0.019 . . . . . . . 26525 2 19 . 1 1 26 26 LYS N N 15 5.819 0.021 . . . . . . . 26525 2 20 . 1 1 27 27 GLU N N 15 5.747 0.015 . . . . . . . 26525 2 21 . 1 1 28 28 GLY N N 15 5.581 0.020 . . . . . . . 26525 2 22 . 1 1 29 29 GLU N N 15 5.877 0.021 . . . . . . . 26525 2 23 . 1 1 31 31 ILE N N 15 5.516 0.064 . . . . . . . 26525 2 24 . 1 1 33 33 LEU N N 15 6.373 0.057 . . . . . . . 26525 2 25 . 1 1 34 34 ILE N N 15 6.038 0.034 . . . . . . . 26525 2 26 . 1 1 35 35 SER N N 15 6.056 0.081 . . . . . . . 26525 2 27 . 1 1 37 37 GLU N N 15 5.713 0.034 . . . . . . . 26525 2 28 . 1 1 38 38 THR N N 15 5.329 0.036 . . . . . . . 26525 2 29 . 1 1 39 39 GLY N N 15 5.339 0.062 . . . . . . . 26525 2 30 . 1 1 40 40 GLU N N 15 5.360 0.017 . . . . . . . 26525 2 31 . 1 1 41 41 ALA N N 15 5.460 0.041 . . . . . . . 26525 2 32 . 1 1 43 43 TRP N N 15 5.806 0.023 . . . . . . . 26525 2 33 . 1 1 45 45 ARG N N 15 5.974 0.021 . . . . . . . 26525 2 34 . 1 1 46 46 GLY N N 15 6.264 0.033 . . . . . . . 26525 2 35 . 1 1 47 47 GLU N N 15 5.711 0.017 . . . . . . . 26525 2 36 . 1 1 48 48 LEU N N 15 5.843 0.020 . . . . . . . 26525 2 37 . 1 1 49 49 ASN N N 15 6.032 0.085 . . . . . . . 26525 2 38 . 1 1 50 50 GLY N N 15 5.555 0.036 . . . . . . . 26525 2 39 . 1 1 51 51 LYS N N 15 5.448 0.015 . . . . . . . 26525 2 40 . 1 1 53 53 GLY N N 15 6.039 0.032 . . . . . . . 26525 2 41 . 1 1 54 54 VAL N N 15 5.874 0.047 . . . . . . . 26525 2 42 . 1 1 55 55 PHE N N 15 5.486 0.026 . . . . . . . 26525 2 43 . 1 1 57 57 ASP N N 15 7.109 0.037 . . . . . . . 26525 2 44 . 1 1 58 58 ASN N N 15 5.435 0.015 . . . . . . . 26525 2 45 . 1 1 59 59 PHE N N 15 5.879 0.019 . . . . . . . 26525 2 46 . 1 1 60 60 ALA N N 15 5.809 0.013 . . . . . . . 26525 2 47 . 1 1 61 61 VAL N N 15 5.546 0.016 . . . . . . . 26525 2 48 . 1 1 63 63 ILE N N 15 5.470 0.020 . . . . . . . 26525 2 49 . 1 1 64 64 ASN N N 15 4.346 0.012 . . . . . . . 26525 2 stop_ save_ save_heteronuclear_T2_list_3 _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronuclear_T2_list_3 _Heteronucl_T2_list.Entry_ID 26525 _Heteronucl_T2_list.ID 3 _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method 'no calibration applied' _Heteronucl_T2_list.Temp_control_method 'no temperature control applied' _Heteronucl_T2_list.Spectrometer_frequency_1H 600 _Heteronucl_T2_list.T2_coherence_type S(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details ; Ubiquitin percent bound = 53.8 % SH3 pecent bound = 36.0 % ; _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 4 '1H-15N HSQC 4' . . . 26525 3 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 1 1 5 5 ALA N N 15 2.874 0.068 . . . . . . . 26525 3 2 . 1 1 6 6 LYS N N 15 3.691 0.051 . . . . . . . 26525 3 3 . 1 1 7 7 GLU N N 15 5.382 0.055 . . . . . . . 26525 3 4 . 1 1 8 8 TYR N N 15 5.945 0.048 . . . . . . . 26525 3 5 . 1 1 9 9 CYS N N 15 6.415 0.078 . . . . . . . 26525 3 6 . 1 1 10 10 ARG N N 15 6.208 0.056 . . . . . . . 26525 3 7 . 1 1 11 11 THR N N 15 6.657 0.045 . . . . . . . 26525 3 8 . 1 1 13 13 PHE N N 15 6.503 0.041 . . . . . . . 26525 3 9 . 1 1 15 15 TYR N N 15 7.684 0.060 . . . . . . . 26525 3 10 . 1 1 16 16 GLU N N 15 6.410 0.051 . . . . . . . 26525 3 11 . 1 1 17 17 GLY N N 15 6.312 0.055 . . . . . . . 26525 3 12 . 1 1 18 18 THR N N 15 7.240 0.063 . . . . . . . 26525 3 13 . 1 1 19 19 ASN N N 15 5.833 0.048 . . . . . . . 26525 3 14 . 1 1 20 20 ASN N N 15 8.930 0.170 . . . . . . . 26525 3 15 . 1 1 21 21 ASP N N 15 7.339 0.056 . . . . . . . 26525 3 16 . 1 1 23 23 LEU N N 15 6.286 0.053 . . . . . . . 26525 3 17 . 1 1 24 24 THR N N 15 5.827 0.074 . . . . . . . 26525 3 18 . 1 1 25 25 PHE N N 15 7.074 0.048 . . . . . . . 26525 3 19 . 1 1 26 26 LYS N N 15 6.540 0.066 . . . . . . . 26525 3 20 . 1 1 27 27 GLU N N 15 6.403 0.038 . . . . . . . 26525 3 21 . 1 1 28 28 GLY N N 15 6.304 0.063 . . . . . . . 26525 3 22 . 1 1 29 29 GLU N N 15 6.696 0.053 . . . . . . . 26525 3 23 . 1 1 31 31 ILE N N 15 6.119 0.061 . . . . . . . 26525 3 24 . 1 1 33 33 LEU N N 15 7.045 0.118 . . . . . . . 26525 3 25 . 1 1 34 34 ILE N N 15 6.663 0.100 . . . . . . . 26525 3 26 . 1 1 35 35 SER N N 15 6.698 0.083 . . . . . . . 26525 3 27 . 1 1 37 37 GLU N N 15 6.581 0.052 . . . . . . . 26525 3 28 . 1 1 38 38 THR N N 15 6.191 0.082 . . . . . . . 26525 3 29 . 1 1 39 39 GLY N N 15 6.090 0.113 . . . . . . . 26525 3 30 . 1 1 40 40 GLU N N 15 6.203 0.045 . . . . . . . 26525 3 31 . 1 1 41 41 ALA N N 15 6.099 0.063 . . . . . . . 26525 3 32 . 1 1 43 43 TRP N N 15 6.520 0.063 . . . . . . . 26525 3 33 . 1 1 45 45 ARG N N 15 6.680 0.065 . . . . . . . 26525 3 34 . 1 1 46 46 GLY N N 15 7.004 0.067 . . . . . . . 26525 3 35 . 1 1 47 47 GLU N N 15 6.447 0.052 . . . . . . . 26525 3 36 . 1 1 48 48 LEU N N 15 6.508 0.059 . . . . . . . 26525 3 37 . 1 1 49 49 ASN N N 15 6.572 0.157 . . . . . . . 26525 3 38 . 1 1 50 50 GLY N N 15 6.007 0.076 . . . . . . . 26525 3 39 . 1 1 51 51 LYS N N 15 6.133 0.044 . . . . . . . 26525 3 40 . 1 1 53 53 GLY N N 15 6.740 0.067 . . . . . . . 26525 3 41 . 1 1 54 54 VAL N N 15 6.660 0.088 . . . . . . . 26525 3 42 . 1 1 55 55 PHE N N 15 6.232 0.100 . . . . . . . 26525 3 43 . 1 1 57 57 ASP N N 15 8.434 0.069 . . . . . . . 26525 3 44 . 1 1 58 58 ASN N N 15 6.157 0.047 . . . . . . . 26525 3 45 . 1 1 59 59 PHE N N 15 6.785 0.073 . . . . . . . 26525 3 46 . 1 1 60 60 ALA N N 15 6.519 0.048 . . . . . . . 26525 3 47 . 1 1 61 61 VAL N N 15 6.144 0.052 . . . . . . . 26525 3 48 . 1 1 63 63 ILE N N 15 6.155 0.063 . . . . . . . 26525 3 49 . 1 1 64 64 ASN N N 15 4.666 0.030 . . . . . . . 26525 3 stop_ save_ save_heteronuclear_T2_list_4 _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronuclear_T2_list_4 _Heteronucl_T2_list.Entry_ID 26525 _Heteronucl_T2_list.ID 4 _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method 'no calibration applied' _Heteronucl_T2_list.Temp_control_method 'no temperature control applied' _Heteronucl_T2_list.Spectrometer_frequency_1H 600 _Heteronucl_T2_list.T2_coherence_type S(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details ; Ubiquitin percent bound = 28.4 % SH3 pecent bound = 59.3 % ; _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 5 '1H-15N HSQC 5' . . . 26525 4 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 1 1 5 5 ALA N N 15 3.130 0.108 . . . . . . . 26525 4 2 . 1 1 6 6 LYS N N 15 4.188 0.063 . . . . . . . 26525 4 3 . 1 1 7 7 GLU N N 15 6.299 0.060 . . . . . . . 26525 4 4 . 1 1 8 8 TYR N N 15 7.138 0.086 . . . . . . . 26525 4 5 . 1 1 9 9 CYS N N 15 7.183 0.092 . . . . . . . 26525 4 6 . 1 1 10 10 ARG N N 15 7.319 0.089 . . . . . . . 26525 4 7 . 1 1 11 11 THR N N 15 7.830 0.076 . . . . . . . 26525 4 8 . 1 1 13 13 PHE N N 15 7.598 0.085 . . . . . . . 26525 4 9 . 1 1 15 15 TYR N N 15 8.711 0.091 . . . . . . . 26525 4 10 . 1 1 16 16 GLU N N 15 7.345 0.082 . . . . . . . 26525 4 11 . 1 1 17 17 GLY N N 15 7.392 0.093 . . . . . . . 26525 4 12 . 1 1 18 18 THR N N 15 8.277 0.110 . . . . . . . 26525 4 13 . 1 1 19 19 ASN N N 15 6.416 0.107 . . . . . . . 26525 4 14 . 1 1 20 20 ASN N N 15 9.780 0.322 . . . . . . . 26525 4 15 . 1 1 21 21 ASP N N 15 8.479 0.094 . . . . . . . 26525 4 16 . 1 1 23 23 LEU N N 15 7.241 0.127 . . . . . . . 26525 4 17 . 1 1 24 24 THR N N 15 6.712 0.083 . . . . . . . 26525 4 18 . 1 1 25 25 PHE N N 15 8.457 0.083 . . . . . . . 26525 4 19 . 1 1 26 26 LYS N N 15 7.686 0.097 . . . . . . . 26525 4 20 . 1 1 27 27 GLU N N 15 7.522 0.066 . . . . . . . 26525 4 21 . 1 1 28 28 GLY N N 15 7.169 0.096 . . . . . . . 26525 4 22 . 1 1 29 29 GLU N N 15 7.823 0.073 . . . . . . . 26525 4 23 . 1 1 31 31 ILE N N 15 6.957 0.113 . . . . . . . 26525 4 24 . 1 1 33 33 LEU N N 15 8.040 0.131 . . . . . . . 26525 4 25 . 1 1 34 34 ILE N N 15 7.706 0.213 . . . . . . . 26525 4 26 . 1 1 35 35 SER N N 15 7.738 0.095 . . . . . . . 26525 4 27 . 1 1 37 37 GLU N N 15 7.314 0.081 . . . . . . . 26525 4 28 . 1 1 38 38 THR N N 15 6.927 0.136 . . . . . . . 26525 4 29 . 1 1 39 39 GLY N N 15 7.254 0.182 . . . . . . . 26525 4 30 . 1 1 40 40 GLU N N 15 7.458 0.076 . . . . . . . 26525 4 31 . 1 1 41 41 ALA N N 15 7.284 0.075 . . . . . . . 26525 4 32 . 1 1 43 43 TRP N N 15 7.487 0.093 . . . . . . . 26525 4 33 . 1 1 45 45 ARG N N 15 7.600 0.090 . . . . . . . 26525 4 34 . 1 1 46 46 GLY N N 15 8.021 0.091 . . . . . . . 26525 4 35 . 1 1 47 47 GLU N N 15 7.398 0.081 . . . . . . . 26525 4 36 . 1 1 48 48 LEU N N 15 7.514 0.112 . . . . . . . 26525 4 37 . 1 1 49 49 ASN N N 15 7.405 0.216 . . . . . . . 26525 4 38 . 1 1 50 50 GLY N N 15 6.942 0.093 . . . . . . . 26525 4 39 . 1 1 51 51 LYS N N 15 7.030 0.080 . . . . . . . 26525 4 40 . 1 1 53 53 GLY N N 15 7.821 0.080 . . . . . . . 26525 4 41 . 1 1 54 54 VAL N N 15 7.476 0.129 . . . . . . . 26525 4 42 . 1 1 55 55 PHE N N 15 6.869 0.141 . . . . . . . 26525 4 43 . 1 1 57 57 ASP N N 15 9.528 0.118 . . . . . . . 26525 4 44 . 1 1 58 58 ASN N N 15 7.118 0.057 . . . . . . . 26525 4 45 . 1 1 59 59 PHE N N 15 7.830 0.080 . . . . . . . 26525 4 46 . 1 1 60 60 ALA N N 15 7.407 0.060 . . . . . . . 26525 4 47 . 1 1 61 61 VAL N N 15 7.163 0.070 . . . . . . . 26525 4 48 . 1 1 63 63 ILE N N 15 7.178 0.091 . . . . . . . 26525 4 49 . 1 1 64 64 ASN N N 15 5.269 0.038 . . . . . . . 26525 4 stop_ save_ save_heteronuclear_T2_list_5 _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronuclear_T2_list_5 _Heteronucl_T2_list.Entry_ID 26525 _Heteronucl_T2_list.ID 5 _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method 'no calibration applied' _Heteronucl_T2_list.Temp_control_method 'no temperature control applied' _Heteronucl_T2_list.Spectrometer_frequency_1H 600 _Heteronucl_T2_list.T2_coherence_type S(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details . _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 2 '1H-15N HSQC 2' . . . 26525 5 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 2 2 8 8 GLN N N 15 5.965 0.052 . . . 2 2 GLN N 26525 5 2 . 2 2 10 10 PHE N N 15 5.949 0.052 . . . 2 4 PHE N 26525 5 3 . 2 2 11 11 VAL N N 15 5.697 0.061 . . . 2 5 VAL N 26525 5 4 . 2 2 12 12 LYS N N 15 5.748 0.098 . . . 2 6 LYS N 26525 5 5 . 2 2 13 13 THR N N 15 5.861 0.069 . . . 2 7 THR N 26525 5 6 . 2 2 14 14 LEU N N 15 5.568 0.088 . . . 2 8 LEU N 26525 5 7 . 2 2 15 15 THR N N 15 5.509 0.264 . . . 2 9 THR N 26525 5 8 . 2 2 16 16 GLY N N 15 5.382 0.109 . . . 2 10 GLY N 26525 5 9 . 2 2 17 17 LYS N N 15 5.127 0.059 . . . 2 11 LYS N 26525 5 10 . 2 2 18 18 THR N N 15 5.401 0.110 . . . 2 12 THR N 26525 5 11 . 2 2 19 19 ILE N N 15 5.682 0.071 . . . 2 13 ILE N 26525 5 12 . 2 2 20 20 THR N N 15 5.721 0.049 . . . 2 14 THR N 26525 5 13 . 2 2 21 21 LEU N N 15 5.675 0.079 . . . 2 15 LEU N 26525 5 14 . 2 2 22 22 GLU N N 15 5.555 0.048 . . . 2 16 GLU N 26525 5 15 . 2 2 23 23 VAL N N 15 6.007 0.053 . . . 2 17 VAL N 26525 5 16 . 2 2 24 24 GLU N N 15 6.393 0.055 . . . 2 18 GLU N 26525 5 17 . 2 2 26 26 SER N N 15 5.786 0.105 . . . 2 20 SER N 26525 5 18 . 2 2 28 28 THR N N 15 5.947 0.068 . . . 2 22 THR N 26525 5 19 . 2 2 31 31 ASN N N 15 7.563 0.067 . . . 2 25 ASN N 26525 5 20 . 2 2 32 32 VAL N N 15 5.799 0.047 . . . 2 26 VAL N 26525 5 21 . 2 2 33 33 LYS N N 15 6.391 0.063 . . . 2 27 LYS N 26525 5 22 . 2 2 34 34 ALA N N 15 6.309 0.061 . . . 2 28 ALA N 26525 5 23 . 2 2 35 35 LYS N N 15 6.087 0.050 . . . 2 29 LYS N 26525 5 24 . 2 2 36 36 ILE N N 15 5.913 0.044 . . . 2 30 ILE N 26525 5 25 . 2 2 37 37 GLN N N 15 6.077 0.055 . . . 2 31 GLN N 26525 5 26 . 2 2 38 38 ASP N N 15 6.271 0.041 . . . 2 32 ASP N 26525 5 27 . 2 2 39 39 LYS N N 15 5.659 0.075 . . . 2 33 LYS N 26525 5 28 . 2 2 40 40 GLU N N 15 5.670 0.058 . . . 2 34 GLU N 26525 5 29 . 2 2 41 41 GLY N N 15 6.819 0.102 . . . 2 35 GLY N 26525 5 30 . 2 2 42 42 ILE N N 15 5.946 0.050 . . . 2 36 ILE N 26525 5 31 . 2 2 45 45 ASP N N 15 5.697 0.043 . . . 2 39 ASP N 26525 5 32 . 2 2 46 46 GLN N N 15 5.898 0.062 . . . 2 40 GLN N 26525 5 33 . 2 2 47 47 GLN N N 15 5.829 0.072 . . . 2 41 GLN N 26525 5 34 . 2 2 48 48 ARG N N 15 5.462 0.082 . . . 2 42 ARG N 26525 5 35 . 2 2 49 49 LEU N N 15 5.721 0.056 . . . 2 43 LEU N 26525 5 36 . 2 2 50 50 ILE N N 15 5.614 0.077 . . . 2 44 ILE N 26525 5 37 . 2 2 51 51 PHE N N 15 6.019 0.117 . . . 2 45 PHE N 26525 5 38 . 2 2 52 52 ALA N N 15 6.126 0.213 . . . 2 46 ALA N 26525 5 39 . 2 2 53 53 GLY N N 15 5.457 0.153 . . . 2 47 GLY N 26525 5 40 . 2 2 54 54 LYS N N 15 6.280 0.071 . . . 2 48 LYS N 26525 5 41 . 2 2 55 55 GLN N N 15 5.274 0.037 . . . 2 49 GLN N 26525 5 42 . 2 2 57 57 GLU N N 15 5.909 0.061 . . . 2 51 GLU N 26525 5 43 . 2 2 58 58 ASP N N 15 6.207 0.046 . . . 2 52 ASP N 26525 5 44 . 2 2 59 59 GLY N N 15 5.889 0.058 . . . 2 53 GLY N 26525 5 45 . 2 2 60 60 ARG N N 15 5.953 0.083 . . . 2 54 ARG N 26525 5 46 . 2 2 61 61 THR N N 15 6.374 0.126 . . . 2 55 THR N 26525 5 47 . 2 2 62 62 LEU N N 15 5.905 0.047 . . . 2 56 LEU N 26525 5 48 . 2 2 63 63 SER N N 15 5.874 0.049 . . . 2 57 SER N 26525 5 49 . 2 2 65 65 TYR N N 15 5.775 0.062 . . . 2 59 TYR N 26525 5 50 . 2 2 66 66 ASN N N 15 5.826 0.073 . . . 2 60 ASN N 26525 5 51 . 2 2 67 67 ILE N N 15 5.871 0.056 . . . 2 61 ILE N 26525 5 52 . 2 2 68 68 GLN N N 15 4.894 0.043 . . . 2 62 GLN N 26525 5 53 . 2 2 69 69 LYS N N 15 5.972 0.054 . . . 2 63 LYS N 26525 5 54 . 2 2 70 70 GLU N N 15 5.742 0.054 . . . 2 64 GLU N 26525 5 55 . 2 2 71 71 SER N N 15 5.834 0.052 . . . 2 65 SER N 26525 5 56 . 2 2 72 72 THR N N 15 5.620 0.066 . . . 2 66 THR N 26525 5 57 . 2 2 74 74 HIS N N 15 5.867 0.058 . . . 2 68 HIS N 26525 5 58 . 2 2 75 75 LEU N N 15 5.765 0.074 . . . 2 69 LEU N 26525 5 59 . 2 2 76 76 VAL N N 15 6.033 0.065 . . . 2 70 VAL N 26525 5 60 . 2 2 77 77 LEU N N 15 5.517 0.047 . . . 2 71 LEU N 26525 5 61 . 2 2 78 78 ARG N N 15 5.213 0.063 . . . 2 72 ARG N 26525 5 62 . 2 2 79 79 LEU N N 15 4.188 0.060 . . . 2 73 LEU N 26525 5 63 . 2 2 80 80 ARG N N 15 4.034 0.124 . . . 2 74 ARG N 26525 5 64 . 2 2 81 81 GLY N N 15 3.209 0.216 . . . 2 75 GLY N 26525 5 65 . 2 2 82 82 GLY N N 15 3.047 0.139 . . . 2 76 GLY N 26525 5 stop_ save_ save_heteronuclear_T2_list_6 _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronuclear_T2_list_6 _Heteronucl_T2_list.Entry_ID 26525 _Heteronucl_T2_list.ID 6 _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method 'no calibration applied' _Heteronucl_T2_list.Temp_control_method 'no temperature control applied' _Heteronucl_T2_list.Spectrometer_frequency_1H 600 _Heteronucl_T2_list.T2_coherence_type S(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details ; Ubiquitin percent bound = 69.5 % SH3 pecent bound = 21.7 % ; _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 3 '1H-15N HSQC 3' . . . 26525 6 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 2 2 8 8 GLN N N 15 7.792 0.109 . . . 2 2 GLN N 26525 6 2 . 2 2 10 10 PHE N N 15 8.142 0.114 . . . 2 4 PHE N 26525 6 3 . 2 2 11 11 VAL N N 15 7.174 0.095 . . . 2 5 VAL N 26525 6 4 . 2 2 12 12 LYS N N 15 7.687 0.174 . . . 2 6 LYS N 26525 6 5 . 2 2 13 13 THR N N 15 7.624 0.115 . . . 2 7 THR N 26525 6 6 . 2 2 14 14 LEU N N 15 7.654 0.238 . . . 2 8 LEU N 26525 6 7 . 2 2 15 15 THR N N 15 11.340 2.173 . . . 2 9 THR N 26525 6 8 . 2 2 16 16 GLY N N 15 6.922 0.236 . . . 2 10 GLY N 26525 6 9 . 2 2 17 17 LYS N N 15 6.886 0.135 . . . 2 11 LYS N 26525 6 10 . 2 2 18 18 THR N N 15 7.192 0.163 . . . 2 12 THR N 26525 6 11 . 2 2 19 19 ILE N N 15 7.431 0.189 . . . 2 13 ILE N 26525 6 12 . 2 2 20 20 THR N N 15 7.989 0.123 . . . 2 14 THR N 26525 6 13 . 2 2 21 21 LEU N N 15 7.307 0.114 . . . 2 15 LEU N 26525 6 14 . 2 2 22 22 GLU N N 15 7.067 0.076 . . . 2 16 GLU N 26525 6 15 . 2 2 23 23 VAL N N 15 7.632 0.105 . . . 2 17 VAL N 26525 6 16 . 2 2 24 24 GLU N N 15 7.775 0.094 . . . 2 18 GLU N 26525 6 17 . 2 2 26 26 SER N N 15 7.342 0.108 . . . 2 20 SER N 26525 6 18 . 2 2 28 28 THR N N 15 7.852 0.170 . . . 2 22 THR N 26525 6 19 . 2 2 31 31 ASN N N 15 9.537 0.197 . . . 2 25 ASN N 26525 6 20 . 2 2 32 32 VAL N N 15 7.280 0.076 . . . 2 26 VAL N 26525 6 21 . 2 2 33 33 LYS N N 15 8.046 0.134 . . . 2 27 LYS N 26525 6 22 . 2 2 34 34 ALA N N 15 7.821 0.076 . . . 2 28 ALA N 26525 6 23 . 2 2 35 35 LYS N N 15 8.062 0.137 . . . 2 29 LYS N 26525 6 24 . 2 2 36 36 ILE N N 15 8.030 0.112 . . . 2 30 ILE N 26525 6 25 . 2 2 37 37 GLN N N 15 7.894 0.091 . . . 2 31 GLN N 26525 6 26 . 2 2 38 38 ASP N N 15 7.823 0.096 . . . 2 32 ASP N 26525 6 27 . 2 2 39 39 LYS N N 15 7.156 0.091 . . . 2 33 LYS N 26525 6 28 . 2 2 40 40 GLU N N 15 7.309 0.096 . . . 2 34 GLU N 26525 6 29 . 2 2 41 41 GLY N N 15 8.623 0.278 . . . 2 35 GLY N 26525 6 30 . 2 2 42 42 ILE N N 15 7.573 0.085 . . . 2 36 ILE N 26525 6 31 . 2 2 45 45 ASP N N 15 7.448 0.098 . . . 2 39 ASP N 26525 6 32 . 2 2 46 46 GLN N N 15 8.302 0.128 . . . 2 40 GLN N 26525 6 33 . 2 2 47 47 GLN N N 15 8.701 0.155 . . . 2 41 GLN N 26525 6 34 . 2 2 48 48 ARG N N 15 8.101 0.269 . . . 2 42 ARG N 26525 6 35 . 2 2 49 49 LEU N N 15 7.557 0.156 . . . 2 43 LEU N 26525 6 36 . 2 2 50 50 ILE N N 15 7.770 0.140 . . . 2 44 ILE N 26525 6 37 . 2 2 51 51 PHE N N 15 8.911 0.166 . . . 2 45 PHE N 26525 6 38 . 2 2 52 52 ALA N N 15 9.029 0.565 . . . 2 46 ALA N 26525 6 39 . 2 2 53 53 GLY N N 15 8.687 0.293 . . . 2 47 GLY N 26525 6 40 . 2 2 54 54 LYS N N 15 8.323 0.185 . . . 2 48 LYS N 26525 6 41 . 2 2 55 55 GLN N N 15 7.160 0.090 . . . 2 49 GLN N 26525 6 42 . 2 2 57 57 GLU N N 15 7.642 0.134 . . . 2 51 GLU N 26525 6 43 . 2 2 58 58 ASP N N 15 7.886 0.077 . . . 2 52 ASP N 26525 6 44 . 2 2 59 59 GLY N N 15 7.831 0.125 . . . 2 53 GLY N 26525 6 45 . 2 2 60 60 ARG N N 15 7.957 0.197 . . . 2 54 ARG N 26525 6 46 . 2 2 61 61 THR N N 15 7.751 0.161 . . . 2 55 THR N 26525 6 47 . 2 2 62 62 LEU N N 15 7.880 0.155 . . . 2 56 LEU N 26525 6 48 . 2 2 63 63 SER N N 15 7.947 0.198 . . . 2 57 SER N 26525 6 49 . 2 2 65 65 TYR N N 15 7.343 0.107 . . . 2 59 TYR N 26525 6 50 . 2 2 66 66 ASN N N 15 7.871 0.112 . . . 2 60 ASN N 26525 6 51 . 2 2 67 67 ILE N N 15 7.773 0.109 . . . 2 61 ILE N 26525 6 52 . 2 2 68 68 GLN N N 15 6.469 0.101 . . . 2 62 GLN N 26525 6 53 . 2 2 69 69 LYS N N 15 7.917 0.117 . . . 2 63 LYS N 26525 6 54 . 2 2 70 70 GLU N N 15 7.829 0.141 . . . 2 64 GLU N 26525 6 55 . 2 2 71 71 SER N N 15 7.803 0.098 . . . 2 65 SER N 26525 6 56 . 2 2 72 72 THR N N 15 7.931 0.153 . . . 2 66 THR N 26525 6 57 . 2 2 74 74 HIS N N 15 7.740 0.193 . . . 2 68 HIS N 26525 6 58 . 2 2 75 75 LEU N N 15 7.539 0.112 . . . 2 69 LEU N 26525 6 59 . 2 2 76 76 VAL N N 15 8.423 0.178 . . . 2 70 VAL N 26525 6 60 . 2 2 77 77 LEU N N 15 7.915 0.156 . . . 2 71 LEU N 26525 6 61 . 2 2 78 78 ARG N N 15 8.038 0.177 . . . 2 72 ARG N 26525 6 62 . 2 2 79 79 LEU N N 15 9.537 0.535 . . . 2 73 LEU N 26525 6 63 . 2 2 80 80 ARG N N 15 5.437 0.205 . . . 2 74 ARG N 26525 6 64 . 2 2 81 81 GLY N N 15 4.758 0.417 . . . 2 75 GLY N 26525 6 65 . 2 2 82 82 GLY N N 15 3.811 0.213 . . . 2 76 GLY N 26525 6 stop_ save_ save_heteronuclear_T2_list_7 _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronuclear_T2_list_7 _Heteronucl_T2_list.Entry_ID 26525 _Heteronucl_T2_list.ID 7 _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method 'no calibration applied' _Heteronucl_T2_list.Temp_control_method 'no temperature control applied' _Heteronucl_T2_list.Spectrometer_frequency_1H 600 _Heteronucl_T2_list.T2_coherence_type S(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details ; Ubiquitin percent bound = 53.8 % SH3 pecent bound = 36.0 % ; _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 4 '1H-15N HSQC 4' . . . 26525 7 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 2 2 8 8 GLN N N 15 7.510 0.073 . . . 2 2 GLN N 26525 7 2 . 2 2 10 10 PHE N N 15 7.602 0.122 . . . 2 4 PHE N 26525 7 3 . 2 2 11 11 VAL N N 15 7.097 0.075 . . . 2 5 VAL N 26525 7 4 . 2 2 12 12 LYS N N 15 7.238 0.124 . . . 2 6 LYS N 26525 7 5 . 2 2 13 13 THR N N 15 7.674 0.121 . . . 2 7 THR N 26525 7 6 . 2 2 14 14 LEU N N 15 7.420 0.225 . . . 2 8 LEU N 26525 7 7 . 2 2 15 15 THR N N 15 10.182 0.886 . . . 2 9 THR N 26525 7 8 . 2 2 16 16 GLY N N 15 6.469 0.121 . . . 2 10 GLY N 26525 7 9 . 2 2 17 17 LYS N N 15 6.585 0.106 . . . 2 11 LYS N 26525 7 10 . 2 2 18 18 THR N N 15 6.882 0.193 . . . 2 12 THR N 26525 7 11 . 2 2 19 19 ILE N N 15 7.387 0.130 . . . 2 13 ILE N 26525 7 12 . 2 2 20 20 THR N N 15 7.426 0.083 . . . 2 14 THR N 26525 7 13 . 2 2 21 21 LEU N N 15 7.173 0.089 . . . 2 15 LEU N 26525 7 14 . 2 2 22 22 GLU N N 15 6.822 0.060 . . . 2 16 GLU N 26525 7 15 . 2 2 23 23 VAL N N 15 7.415 0.082 . . . 2 17 VAL N 26525 7 16 . 2 2 24 24 GLU N N 15 7.843 0.086 . . . 2 18 GLU N 26525 7 17 . 2 2 26 26 SER N N 15 7.356 0.106 . . . 2 20 SER N 26525 7 18 . 2 2 28 28 THR N N 15 7.578 0.112 . . . 2 22 THR N 26525 7 19 . 2 2 31 31 ASN N N 15 9.159 0.111 . . . 2 25 ASN N 26525 7 20 . 2 2 32 32 VAL N N 15 7.222 0.067 . . . 2 26 VAL N 26525 7 21 . 2 2 33 33 LYS N N 15 7.713 0.099 . . . 2 27 LYS N 26525 7 22 . 2 2 34 34 ALA N N 15 7.746 0.064 . . . 2 28 ALA N 26525 7 23 . 2 2 35 35 LYS N N 15 7.493 0.098 . . . 2 29 LYS N 26525 7 24 . 2 2 36 36 ILE N N 15 7.586 0.081 . . . 2 30 ILE N 26525 7 25 . 2 2 37 37 GLN N N 15 7.635 0.089 . . . 2 31 GLN N 26525 7 26 . 2 2 38 38 ASP N N 15 7.655 0.058 . . . 2 32 ASP N 26525 7 27 . 2 2 39 39 LYS N N 15 6.926 0.078 . . . 2 33 LYS N 26525 7 28 . 2 2 40 40 GLU N N 15 7.028 0.105 . . . 2 34 GLU N 26525 7 29 . 2 2 41 41 GLY N N 15 8.411 0.133 . . . 2 35 GLY N 26525 7 30 . 2 2 42 42 ILE N N 15 7.126 0.081 . . . 2 36 ILE N 26525 7 31 . 2 2 45 45 ASP N N 15 7.243 0.074 . . . 2 39 ASP N 26525 7 32 . 2 2 46 46 GLN N N 15 7.777 0.114 . . . 2 40 GLN N 26525 7 33 . 2 2 47 47 GLN N N 15 7.947 0.113 . . . 2 41 GLN N 26525 7 34 . 2 2 48 48 ARG N N 15 7.358 0.165 . . . 2 42 ARG N 26525 7 35 . 2 2 49 49 LEU N N 15 7.116 0.102 . . . 2 43 LEU N 26525 7 36 . 2 2 50 50 ILE N N 15 7.247 0.100 . . . 2 44 ILE N 26525 7 37 . 2 2 51 51 PHE N N 15 8.264 0.116 . . . 2 45 PHE N 26525 7 38 . 2 2 52 52 ALA N N 15 7.268 0.307 . . . 2 46 ALA N 26525 7 39 . 2 2 53 53 GLY N N 15 7.332 0.201 . . . 2 47 GLY N 26525 7 40 . 2 2 54 54 LYS N N 15 8.069 0.132 . . . 2 48 LYS N 26525 7 41 . 2 2 55 55 GLN N N 15 6.920 0.056 . . . 2 49 GLN N 26525 7 42 . 2 2 57 57 GLU N N 15 7.084 0.100 . . . 2 51 GLU N 26525 7 43 . 2 2 58 58 ASP N N 15 7.613 0.068 . . . 2 52 ASP N 26525 7 44 . 2 2 59 59 GLY N N 15 7.543 0.101 . . . 2 53 GLY N 26525 7 45 . 2 2 60 60 ARG N N 15 7.747 0.122 . . . 2 54 ARG N 26525 7 46 . 2 2 61 61 THR N N 15 7.273 0.150 . . . 2 55 THR N 26525 7 47 . 2 2 62 62 LEU N N 15 7.724 0.105 . . . 2 56 LEU N 26525 7 48 . 2 2 63 63 SER N N 15 7.466 0.077 . . . 2 57 SER N 26525 7 49 . 2 2 65 65 TYR N N 15 7.267 0.083 . . . 2 59 TYR N 26525 7 50 . 2 2 66 66 ASN N N 15 7.341 0.088 . . . 2 60 ASN N 26525 7 51 . 2 2 67 67 ILE N N 15 7.361 0.074 . . . 2 61 ILE N 26525 7 52 . 2 2 68 68 GLN N N 15 6.440 0.071 . . . 2 62 GLN N 26525 7 53 . 2 2 69 69 LYS N N 15 7.522 0.094 . . . 2 63 LYS N 26525 7 54 . 2 2 70 70 GLU N N 15 7.357 0.098 . . . 2 64 GLU N 26525 7 55 . 2 2 71 71 SER N N 15 7.587 0.093 . . . 2 65 SER N 26525 7 56 . 2 2 72 72 THR N N 15 7.136 0.107 . . . 2 66 THR N 26525 7 57 . 2 2 74 74 HIS N N 15 7.589 0.124 . . . 2 68 HIS N 26525 7 58 . 2 2 75 75 LEU N N 15 7.032 0.093 . . . 2 69 LEU N 26525 7 59 . 2 2 76 76 VAL N N 15 7.847 0.110 . . . 2 70 VAL N 26525 7 60 . 2 2 77 77 LEU N N 15 7.190 0.076 . . . 2 71 LEU N 26525 7 61 . 2 2 78 78 ARG N N 15 7.442 0.132 . . . 2 72 ARG N 26525 7 62 . 2 2 79 79 LEU N N 15 8.479 0.272 . . . 2 73 LEU N 26525 7 63 . 2 2 80 80 ARG N N 15 5.019 0.161 . . . 2 74 ARG N 26525 7 64 . 2 2 81 81 GLY N N 15 4.178 0.252 . . . 2 75 GLY N 26525 7 65 . 2 2 82 82 GLY N N 15 3.389 0.151 . . . 2 76 GLY N 26525 7 stop_ save_ save_heteronuclear_T2_list_8 _Heteronucl_T2_list.Sf_category heteronucl_T2_relaxation _Heteronucl_T2_list.Sf_framecode heteronuclear_T2_list_8 _Heteronucl_T2_list.Entry_ID 26525 _Heteronucl_T2_list.ID 8 _Heteronucl_T2_list.Sample_condition_list_ID 1 _Heteronucl_T2_list.Sample_condition_list_label $sample_conditions_1 _Heteronucl_T2_list.Temp_calibration_method 'no calibration applied' _Heteronucl_T2_list.Temp_control_method 'no temperature control applied' _Heteronucl_T2_list.Spectrometer_frequency_1H 600 _Heteronucl_T2_list.T2_coherence_type S(+,-) _Heteronucl_T2_list.T2_val_units s-1 _Heteronucl_T2_list.Rex_units . _Heteronucl_T2_list.Details ; Ubiquitin percent bound = 28.4 % SH3 pecent bound = 59.3 % ; _Heteronucl_T2_list.Text_data_format . _Heteronucl_T2_list.Text_data . loop_ _Heteronucl_T2_experiment.Experiment_ID _Heteronucl_T2_experiment.Experiment_name _Heteronucl_T2_experiment.Sample_ID _Heteronucl_T2_experiment.Sample_label _Heteronucl_T2_experiment.Sample_state _Heteronucl_T2_experiment.Entry_ID _Heteronucl_T2_experiment.Heteronucl_T2_list_ID 5 '1H-15N HSQC 5' . . . 26525 8 stop_ loop_ _T2.ID _T2.Assembly_atom_ID _T2.Entity_assembly_ID _T2.Entity_ID _T2.Comp_index_ID _T2.Seq_ID _T2.Comp_ID _T2.Atom_ID _T2.Atom_type _T2.Atom_isotope_number _T2.T2_val _T2.T2_val_err _T2.Rex_val _T2.Rex_err _T2.Resonance_ID _T2.Auth_entity_assembly_ID _T2.Auth_seq_ID _T2.Auth_comp_ID _T2.Auth_atom_ID _T2.Entry_ID _T2.Heteronucl_T2_list_ID 1 . 2 2 8 8 GLN N N 15 6.810 0.023 . . . 2 2 GLN N 26525 8 2 . 2 2 10 10 PHE N N 15 6.905 0.025 . . . 2 4 PHE N 26525 8 3 . 2 2 11 11 VAL N N 15 6.546 0.024 . . . 2 5 VAL N 26525 8 4 . 2 2 12 12 LYS N N 15 6.544 0.034 . . . 2 6 LYS N 26525 8 5 . 2 2 13 13 THR N N 15 6.746 0.036 . . . 2 7 THR N 26525 8 6 . 2 2 14 14 LEU N N 15 6.480 0.077 . . . 2 8 LEU N 26525 8 7 . 2 2 15 15 THR N N 15 7.934 0.255 . . . 2 9 THR N 26525 8 8 . 2 2 16 16 GLY N N 15 6.073 0.049 . . . 2 10 GLY N 26525 8 9 . 2 2 17 17 LYS N N 15 6.013 0.029 . . . 2 11 LYS N 26525 8 10 . 2 2 18 18 THR N N 15 6.440 0.070 . . . 2 12 THR N 26525 8 11 . 2 2 19 19 ILE N N 15 6.618 0.031 . . . 2 13 ILE N 26525 8 12 . 2 2 20 20 THR N N 15 6.807 0.024 . . . 2 14 THR N 26525 8 13 . 2 2 21 21 LEU N N 15 6.474 0.037 . . . 2 15 LEU N 26525 8 14 . 2 2 22 22 GLU N N 15 6.179 0.017 . . . 2 16 GLU N 26525 8 15 . 2 2 23 23 VAL N N 15 6.854 0.022 . . . 2 17 VAL N 26525 8 16 . 2 2 24 24 GLU N N 15 7.123 0.026 . . . 2 18 GLU N 26525 8 17 . 2 2 26 26 SER N N 15 6.835 0.090 . . . 2 20 SER N 26525 8 18 . 2 2 28 28 THR N N 15 6.800 0.060 . . . 2 22 THR N 26525 8 19 . 2 2 31 31 ASN N N 15 8.681 0.049 . . . 2 25 ASN N 26525 8 20 . 2 2 32 32 VAL N N 15 6.758 0.023 . . . 2 26 VAL N 26525 8 21 . 2 2 33 33 LYS N N 15 7.172 0.029 . . . 2 27 LYS N 26525 8 22 . 2 2 34 34 ALA N N 15 7.113 0.040 . . . 2 28 ALA N 26525 8 23 . 2 2 35 35 LYS N N 15 6.967 0.030 . . . 2 29 LYS N 26525 8 24 . 2 2 36 36 ILE N N 15 6.777 0.021 . . . 2 30 ILE N 26525 8 25 . 2 2 37 37 GLN N N 15 6.940 0.033 . . . 2 31 GLN N 26525 8 26 . 2 2 38 38 ASP N N 15 6.947 0.029 . . . 2 32 ASP N 26525 8 27 . 2 2 39 39 LYS N N 15 6.364 0.040 . . . 2 33 LYS N 26525 8 28 . 2 2 40 40 GLU N N 15 6.396 0.023 . . . 2 34 GLU N 26525 8 29 . 2 2 41 41 GLY N N 15 7.733 0.086 . . . 2 35 GLY N 26525 8 30 . 2 2 42 42 ILE N N 15 6.703 0.026 . . . 2 36 ILE N 26525 8 31 . 2 2 45 45 ASP N N 15 6.590 0.025 . . . 2 39 ASP N 26525 8 32 . 2 2 46 46 GLN N N 15 6.895 0.023 . . . 2 40 GLN N 26525 8 33 . 2 2 47 47 GLN N N 15 7.075 0.034 . . . 2 41 GLN N 26525 8 34 . 2 2 48 48 ARG N N 15 6.651 0.042 . . . 2 42 ARG N 26525 8 35 . 2 2 49 49 LEU N N 15 6.544 0.030 . . . 2 43 LEU N 26525 8 36 . 2 2 50 50 ILE N N 15 6.518 0.026 . . . 2 44 ILE N 26525 8 37 . 2 2 51 51 PHE N N 15 7.374 0.027 . . . 2 45 PHE N 26525 8 38 . 2 2 52 52 ALA N N 15 7.057 0.164 . . . 2 46 ALA N 26525 8 39 . 2 2 53 53 GLY N N 15 6.582 0.055 . . . 2 47 GLY N 26525 8 40 . 2 2 54 54 LYS N N 15 7.305 0.027 . . . 2 48 LYS N 26525 8 41 . 2 2 55 55 GLN N N 15 6.320 0.025 . . . 2 49 GLN N 26525 8 42 . 2 2 57 57 GLU N N 15 6.639 0.026 . . . 2 51 GLU N 26525 8 43 . 2 2 58 58 ASP N N 15 6.866 0.017 . . . 2 52 ASP N 26525 8 44 . 2 2 59 59 GLY N N 15 6.760 0.032 . . . 2 53 GLY N 26525 8 45 . 2 2 60 60 ARG N N 15 6.985 0.060 . . . 2 54 ARG N 26525 8 46 . 2 2 61 61 THR N N 15 7.033 0.071 . . . 2 55 THR N 26525 8 47 . 2 2 62 62 LEU N N 15 6.932 0.038 . . . 2 56 LEU N 26525 8 48 . 2 2 63 63 SER N N 15 6.653 0.025 . . . 2 57 SER N 26525 8 49 . 2 2 65 65 TYR N N 15 6.542 0.028 . . . 2 59 TYR N 26525 8 50 . 2 2 66 66 ASN N N 15 6.715 0.025 . . . 2 60 ASN N 26525 8 51 . 2 2 67 67 ILE N N 15 6.706 0.020 . . . 2 61 ILE N 26525 8 52 . 2 2 68 68 GLN N N 15 5.800 0.020 . . . 2 62 GLN N 26525 8 53 . 2 2 69 69 LYS N N 15 6.892 0.026 . . . 2 63 LYS N 26525 8 54 . 2 2 70 70 GLU N N 15 6.634 0.027 . . . 2 64 GLU N 26525 8 55 . 2 2 71 71 SER N N 15 6.801 0.022 . . . 2 65 SER N 26525 8 56 . 2 2 72 72 THR N N 15 6.602 0.027 . . . 2 66 THR N 26525 8 57 . 2 2 74 74 HIS N N 15 6.814 0.038 . . . 2 68 HIS N 26525 8 58 . 2 2 75 75 LEU N N 15 6.535 0.032 . . . 2 69 LEU N 26525 8 59 . 2 2 76 76 VAL N N 15 7.215 0.032 . . . 2 70 VAL N 26525 8 60 . 2 2 77 77 LEU N N 15 6.403 0.024 . . . 2 71 LEU N 26525 8 61 . 2 2 78 78 ARG N N 15 6.335 0.035 . . . 2 72 ARG N 26525 8 62 . 2 2 79 79 LEU N N 15 7.190 0.074 . . . 2 73 LEU N 26525 8 63 . 2 2 80 80 ARG N N 15 4.570 0.079 . . . 2 74 ARG N 26525 8 64 . 2 2 81 81 GLY N N 15 3.572 0.153 . . . 2 75 GLY N 26525 8 65 . 2 2 82 82 GLY N N 15 3.463 0.080 . . . 2 76 GLY N 26525 8 stop_ save_