data_17339 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 17339 _Entry.Title ; The Solution Structure of a Fungal AREA Protein-DNA Complex: An Alternative Binding Mode for a Basic Carboxyl Tail of GATA factors ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2010-12-02 _Entry.Accession_date 2010-12-02 _Entry.Last_release_date 2012-06-06 _Entry.Original_release_date 2012-06-06 _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 3.0.9.13 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 Mary Starich . R. . 17339 2 Mats Wikstrom . . . 17339 3 Herbert Arst . N. Jr. 17339 4 G. Clore . M. . 17339 5 Angela Gronenborn . M. . 17339 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID chemical_rates 1 17339 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'kinetic rates' 1 17339 'binding constants' 6 17339 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2012-06-06 2010-12-02 original author . 17339 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 17339 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 9533883 _Citation.Full_citation . _Citation.Title 'The solution structure of a fungal AREA protein-DNA complex: an alternative binding mode for the basic carboxyl tail of GATA factors.' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'J. Mol. Biol.' _Citation.Journal_name_full 'Journal of molecular biology' _Citation.Journal_volume 277 _Citation.Journal_issue 3 _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 605 _Citation.Page_last 620 _Citation.Year 1998 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Mary Starich . R. . 17339 1 2 Mats Wikstrom . . . 17339 1 3 Herbert Arst . N. Jr. 17339 1 4 G. Clore . M. . 17339 1 5 Angela Gronenborn . M. . 17339 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 17339 _Assembly.ID 1 _Assembly.Name 'AREA/GATA complex' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 AREA 1 $AREA A . yes native no no . . . 17339 1 2 GATA 2 $GATA B . yes native no no . . . 17339 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_AREA _Entity.Sf_category entity _Entity.Sf_framecode AREA _Entity.Entry_ID 17339 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name AREA _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MKNGEQNGPTTCTNCFTQTT PLWRRNPEGQPLCNACGLFL KLHGVVRPLSLKTDVIKKRN RNSANS ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 66 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID 1 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-11-25 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no PDB 2VUS . "Crystal Structure Of Unliganded Nmra-Area Zinc Finger Complex" . . . . . 65.15 43 100.00 100.00 3.39e-22 . . . . 17339 1 2 no PDB 2VUT . "Crystal Structure Of Nad-Bound Nmra-Area Zinc Finger Complex" . . . . . 65.15 43 100.00 100.00 3.39e-22 . . . . 17339 1 3 no PDB 2VUU . "Crystal Structure Of Nadp-Bound Nmra-Area Zinc Finger Complex" . . . . . 65.15 43 100.00 100.00 3.39e-22 . . . . 17339 1 4 no PDB 4GAT . "Solution Nmr Structure Of The Wild Type Dna Binding Domain Of Area Complexed To A 13bp Dna Containing A Cgata Site, Regularized" . . . . . 100.00 66 100.00 100.00 1.17e-39 . . . . 17339 1 5 no PDB 5GAT . "Solution Nmr Structure Of The Wild Type Dna Binding Domain Of Area Complexed To A 13bp Dna Containing A Cgata Site, 35 Structur" . . . . . 100.00 66 100.00 100.00 1.17e-39 . . . . 17339 1 6 no PDB 6GAT . "Solution Nmr Structure Of The L22v Mutant Dna Binding Domain Of Area Complexed To A 13 Bp Dna Containing A Tgata Site, Regulari" . . . . . 100.00 66 98.48 100.00 2.92e-39 . . . . 17339 1 7 no PDB 7GAT . "Solution Nmr Structure Of The L22v Mutant Dna Binding Domain Of Area Complexed To A 13 Bp Dna Containing A Tgata Site, 34 Struc" . . . . . 100.00 66 98.48 100.00 2.92e-39 . . . . 17339 1 8 no DBJ GAN02876 . "hypothetical protein MAM1_0029d02325 [Mucor ambiguus]" . . . . . 57.58 722 97.37 97.37 1.80e-15 . . . . 17339 1 9 no EMBL CAA36731 . "areA [Aspergillus nidulans]" . . . . . 98.48 876 100.00 100.00 3.59e-34 . . . . 17339 1 10 no GB AAU05980 . "global nitrogen regulator protein, partial [Colletotrichum gloeosporioides]" . . . . . 71.21 47 100.00 100.00 2.05e-25 . . . . 17339 1 11 no GB AEO65119 . "hypothetical protein THITE_37279 [Thielavia terrestris NRRL 8126]" . . . . . 80.30 988 100.00 100.00 1.34e-26 . . . . 17339 1 12 no GB EAA60701 . "AREA_EMENI Nitrogen regulatory protein areA [Aspergillus nidulans FGSC A4]" . . . . . 98.48 876 100.00 100.00 3.59e-34 . . . . 17339 1 13 no GB EAW08915 . "GATA transcriptional activator AreA [Aspergillus clavatus NRRL 1]" . . . . . 100.00 879 96.97 98.48 5.16e-32 . . . . 17339 1 14 no GB EGS22804 . "putative sequence-specific DNA binding protein [Chaetomium thermophilum var. thermophilum DSM 1495]" . . . . . 80.30 928 100.00 100.00 7.96e-29 . . . . 17339 1 15 no REF XP_001270341 . "GATA transcriptional activator AreA [Aspergillus clavatus NRRL 1]" . . . . . 100.00 879 96.97 98.48 5.16e-32 . . . . 17339 1 16 no REF XP_003651455 . "hypothetical protein THITE_37279 [Thielavia terrestris NRRL 8126]" . . . . . 80.30 988 100.00 100.00 1.34e-26 . . . . 17339 1 17 no REF XP_006691796 . "putative sequence-specific DNA binding protein [Chaetomium thermophilum var. thermophilum DSM 1495]" . . . . . 80.30 928 100.00 100.00 7.96e-29 . . . . 17339 1 18 no REF XP_681936 . "AREA_EMENI Nitrogen regulatory protein areA [Aspergillus nidulans FGSC A4]" . . . . . 98.48 876 100.00 100.00 3.59e-34 . . . . 17339 1 19 no SP P17429 . "RecName: Full=Nitrogen regulatory protein areA" . . . . . 98.48 876 100.00 100.00 3.59e-34 . . . . 17339 1 20 no TPE CBF78221 . "TPA: Nitrogen regulatory protein areA [Source:UniProtKB/Swiss-Prot;Acc:P17429] [Aspergillus nidulans FGSC A4]" . . . . . 98.48 876 100.00 100.00 3.59e-34 . . . . 17339 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 17339 1 2 . LYS . 17339 1 3 . ASN . 17339 1 4 . GLY . 17339 1 5 . GLU . 17339 1 6 . GLN . 17339 1 7 . ASN . 17339 1 8 . GLY . 17339 1 9 . PRO . 17339 1 10 . THR . 17339 1 11 . THR . 17339 1 12 . CYS . 17339 1 13 . THR . 17339 1 14 . ASN . 17339 1 15 . CYS . 17339 1 16 . PHE . 17339 1 17 . THR . 17339 1 18 . GLN . 17339 1 19 . THR . 17339 1 20 . THR . 17339 1 21 . PRO . 17339 1 22 . LEU . 17339 1 23 . TRP . 17339 1 24 . ARG . 17339 1 25 . ARG . 17339 1 26 . ASN . 17339 1 27 . PRO . 17339 1 28 . GLU . 17339 1 29 . GLY . 17339 1 30 . GLN . 17339 1 31 . PRO . 17339 1 32 . LEU . 17339 1 33 . CYS . 17339 1 34 . ASN . 17339 1 35 . ALA . 17339 1 36 . CYS . 17339 1 37 . GLY . 17339 1 38 . LEU . 17339 1 39 . PHE . 17339 1 40 . LEU . 17339 1 41 . LYS . 17339 1 42 . LEU . 17339 1 43 . HIS . 17339 1 44 . GLY . 17339 1 45 . VAL . 17339 1 46 . VAL . 17339 1 47 . ARG . 17339 1 48 . PRO . 17339 1 49 . LEU . 17339 1 50 . SER . 17339 1 51 . LEU . 17339 1 52 . LYS . 17339 1 53 . THR . 17339 1 54 . ASP . 17339 1 55 . VAL . 17339 1 56 . ILE . 17339 1 57 . LYS . 17339 1 58 . LYS . 17339 1 59 . ARG . 17339 1 60 . ASN . 17339 1 61 . ARG . 17339 1 62 . ASN . 17339 1 63 . SER . 17339 1 64 . ALA . 17339 1 65 . ASN . 17339 1 66 . SER . 17339 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 17339 1 . LYS 2 2 17339 1 . ASN 3 3 17339 1 . GLY 4 4 17339 1 . GLU 5 5 17339 1 . GLN 6 6 17339 1 . ASN 7 7 17339 1 . GLY 8 8 17339 1 . PRO 9 9 17339 1 . THR 10 10 17339 1 . THR 11 11 17339 1 . CYS 12 12 17339 1 . THR 13 13 17339 1 . ASN 14 14 17339 1 . CYS 15 15 17339 1 . PHE 16 16 17339 1 . THR 17 17 17339 1 . GLN 18 18 17339 1 . THR 19 19 17339 1 . THR 20 20 17339 1 . PRO 21 21 17339 1 . LEU 22 22 17339 1 . TRP 23 23 17339 1 . ARG 24 24 17339 1 . ARG 25 25 17339 1 . ASN 26 26 17339 1 . PRO 27 27 17339 1 . GLU 28 28 17339 1 . GLY 29 29 17339 1 . GLN 30 30 17339 1 . PRO 31 31 17339 1 . LEU 32 32 17339 1 . CYS 33 33 17339 1 . ASN 34 34 17339 1 . ALA 35 35 17339 1 . CYS 36 36 17339 1 . GLY 37 37 17339 1 . LEU 38 38 17339 1 . PHE 39 39 17339 1 . LEU 40 40 17339 1 . LYS 41 41 17339 1 . LEU 42 42 17339 1 . HIS 43 43 17339 1 . GLY 44 44 17339 1 . VAL 45 45 17339 1 . VAL 46 46 17339 1 . ARG 47 47 17339 1 . PRO 48 48 17339 1 . LEU 49 49 17339 1 . SER 50 50 17339 1 . LEU 51 51 17339 1 . LYS 52 52 17339 1 . THR 53 53 17339 1 . ASP 54 54 17339 1 . VAL 55 55 17339 1 . ILE 56 56 17339 1 . LYS 57 57 17339 1 . LYS 58 58 17339 1 . ARG 59 59 17339 1 . ASN 60 60 17339 1 . ARG 61 61 17339 1 . ASN 62 62 17339 1 . SER 63 63 17339 1 . ALA 64 64 17339 1 . ASN 65 65 17339 1 . SER 66 66 17339 1 stop_ save_ save_GATA _Entity.Sf_category entity _Entity.Sf_framecode GATA _Entity.Entry_ID 17339 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name GATA _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polydeoxyribonucleotide _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; CAGCGATAGAGACGTCGCTA TCTCTG ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 26 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID 2 _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . DC . 17339 2 2 . DA . 17339 2 3 . DG . 17339 2 4 . DC . 17339 2 5 . DG . 17339 2 6 . DA . 17339 2 7 . DT . 17339 2 8 . DA . 17339 2 9 . DG . 17339 2 10 . DA . 17339 2 11 . DG . 17339 2 12 . DA . 17339 2 13 . DC . 17339 2 14 . DG . 17339 2 15 . DT . 17339 2 16 . DC . 17339 2 17 . DG . 17339 2 18 . DC . 17339 2 19 . DT . 17339 2 20 . DA . 17339 2 21 . DT . 17339 2 22 . DC . 17339 2 23 . DT . 17339 2 24 . DC . 17339 2 25 . DT . 17339 2 26 . DG . 17339 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . DC 1 1 17339 2 . DA 2 2 17339 2 . DG 3 3 17339 2 . DC 4 4 17339 2 . DG 5 5 17339 2 . DA 6 6 17339 2 . DT 7 7 17339 2 . DA 8 8 17339 2 . DG 9 9 17339 2 . DA 10 10 17339 2 . DG 11 11 17339 2 . DA 12 12 17339 2 . DC 13 13 17339 2 . DG 14 14 17339 2 . DT 15 15 17339 2 . DC 16 16 17339 2 . DG 17 17 17339 2 . DC 18 18 17339 2 . DT 19 19 17339 2 . DA 20 20 17339 2 . DT 21 21 17339 2 . DC 22 22 17339 2 . DT 23 23 17339 2 . DC 24 24 17339 2 . DT 25 25 17339 2 . DG 26 26 17339 2 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 17339 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $AREA . 162425 organism . 'Aspergillus nidulans' 'Aspergillus nidulans' . . Eukaryota Fungi Aspergillus nidulans . . . . . . . . . . . . . . . . . . . . . 17339 1 2 2 $GATA . . 'not applicable' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17339 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 17339 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $AREA . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli BL21 DE3 . . . . . . . . . . . . . . pET11a . . . . . . 17339 1 2 2 $GATA . 'obtained from a vendor' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17339 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 17339 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details 'AREA has zinc bound and was in a complex with GATA' _Sample.Aggregate_sample_number . _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 AREA [U-15N] . . 1 $AREA . . 2 . . mM . . . . 17339 1 2 GATA 'natural abundance' . . 2 $GATA . . 2 . . mM . . . . 17339 1 3 'sodium chloride' 'natural abundance' . . . . . . 12 . . mM . . . . 17339 1 4 'zinc chloride' 'natural abundance' . . . . . . 2.2 . . mM . . . . 17339 1 5 'sodium azide' 'natural abundance' . . . . . . 5.0 . . mM . . . . 17339 1 6 H2O 'natural abundance' . . . . . . 90 . . % . . . . 17339 1 7 D2O 'natural abundance' . . . . . . 10 . . % . . . . 17339 1 stop_ save_ save_sample_2 _Sample.Sf_category sample _Sample.Sf_framecode sample_2 _Sample.Entry_ID 17339 _Sample.ID 2 _Sample.Type solution _Sample.Sub_type . _Sample.Details 'AREA has zinc bound and was in a complex with GATA' _Sample.Aggregate_sample_number . _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 AREA '[U-13C; U-15N]' . . 1 $AREA . . 2 . . mM . . . . 17339 2 2 GATA 'natural abundance' . . 2 $GATA . . 2 . . mM . . . . 17339 2 3 'sodium chloride' 'natural abundance' . . . . . . 12 . . mM . . . . 17339 2 4 'zinc chloride' 'natural abundance' . . . . . . 2.2 . . mM . . . . 17339 2 5 'sodium azide' 'natural abundance' . . . . . . 5.0 . . mM . . . . 17339 2 6 H2O 'natural abundance' . . . . . . 90 . . % . . . . 17339 2 7 D2O 'natural abundance' . . . . . . 10 . . % . . . . 17339 2 stop_ save_ save_sample_3 _Sample.Sf_category sample _Sample.Sf_framecode sample_3 _Sample.Entry_ID 17339 _Sample.ID 3 _Sample.Type solution _Sample.Sub_type . _Sample.Details 'AREA has zinc bound and was in a complex with GATA' _Sample.Aggregate_sample_number . _Sample.Solvent_system '99.996% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 AREA '[U-13C; U-15N]' . . 1 $AREA . . 2 . . mM . . . . 17339 3 2 GATA 'natural abundance' . . 2 $GATA . . 2 . . mM . . . . 17339 3 3 'sodium chloride' 'natural abundance' . . . . . . 12 . . mM . . . . 17339 3 4 'zinc chloride' 'natural abundance' . . . . . . 2.2 . . mM . . . . 17339 3 5 'sodium azide' 'natural abundance' . . . . . . 5.0 . . mM . . . . 17339 3 6 D2O 'natural abundance' . . . . . . 99.996 . . % . . . . 17339 3 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 17339 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID temperature 298 . K 17339 1 pH 6.5 . pH 17339 1 pressure 1 . atm 17339 1 stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Software.Sf_category software _Software.Sf_framecode NMRPipe _Software.Entry_ID 17339 _Software.ID 1 _Software.Name NMRPipe _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Johnson, One Moon Scientific' . . 17339 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID processing 17339 1 stop_ save_ save_PIPP _Software.Sf_category software _Software.Sf_framecode PIPP _Software.Entry_ID 17339 _Software.ID 2 _Software.Name PIPP _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID Garrett . . 17339 2 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'data analysis' 17339 2 stop_ save_ save_CAPP _Software.Sf_category software _Software.Sf_framecode CAPP _Software.Entry_ID 17339 _Software.ID 3 _Software.Name CAPP _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID Garrett . . 17339 3 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'data analysis' 17339 3 stop_ save_ save_STAPP _Software.Sf_category software _Software.Sf_framecode STAPP _Software.Entry_ID 17339 _Software.ID 4 _Software.Name STAPP _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID '(Garrett et al., 1991)' . . 17339 4 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID 'data analysis' 17339 4 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 17339 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model AMX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 500 save_ save_spectrometer_2 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_2 _NMR_spectrometer.Entry_ID 17339 _NMR_spectrometer.ID 2 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model DMX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 500 save_ save_spectrometer_3 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_3 _NMR_spectrometer.Entry_ID 17339 _NMR_spectrometer.ID 3 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model AMX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_spectrometer_4 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_4 _NMR_spectrometer.Entry_ID 17339 _NMR_spectrometer.ID 4 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model DMX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_spectrometer_5 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_5 _NMR_spectrometer.Entry_ID 17339 _NMR_spectrometer.ID 5 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model DMX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 750 save_ save_spectrometer_6 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_6 _NMR_spectrometer.Entry_ID 17339 _NMR_spectrometer.ID 6 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model AMX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 360 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 17339 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Bruker AMX . 500 . . . 17339 1 2 spectrometer_2 Bruker DMX . 500 . . . 17339 1 3 spectrometer_3 Bruker AMX . 600 . . . 17339 1 4 spectrometer_4 Bruker DMX . 600 . . . 17339 1 5 spectrometer_5 Bruker DMX . 750 . . . 17339 1 6 spectrometer_6 Bruker AMX . 360 . . . 17339 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 17339 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . . . . . . . . . . . . . . . . . . . 17339 1 2 '2D 1H-15N HSQC' no . . . . . . . . . . 2 $sample_2 isotropic . . 1 $sample_conditions_1 . . . . . . . . . . . . . . . . . . . . . 17339 1 3 '2D 1H-15N HSQC' no . . . . . . . . . . 3 $sample_3 isotropic . . 1 $sample_conditions_1 . . . . . . . . . . . . . . . . . . . . . 17339 1 stop_ save_ save_Chemical_rate_list _Chemical_rate_list.Sf_category chemical_rates _Chemical_rate_list.Sf_framecode Chemical_rate_list _Chemical_rate_list.Entry_ID 17339 _Chemical_rate_list.ID 1 _Chemical_rate_list.Sample_condition_list_ID 1 _Chemical_rate_list.Sample_condition_list_label $sample_conditions_1 _Chemical_rate_list.Details . _Chemical_rate_list.Text_data_format . _Chemical_rate_list.Text_data . loop_ _Chemical_rate_experiment.Experiment_ID _Chemical_rate_experiment.Experiment_name _Chemical_rate_experiment.Sample_ID _Chemical_rate_experiment.Sample_label _Chemical_rate_experiment.Sample_state _Chemical_rate_experiment.Entry_ID _Chemical_rate_experiment.Chemical_rate_list_ID 1 '2D 1H-15N HSQC' 1 $sample_1 isotropic 17339 1 2 '2D 1H-15N HSQC' 2 $sample_2 isotropic 17339 1 3 '2D 1H-15N HSQC' 3 $sample_3 isotropic 17339 1 stop_ loop_ _Chemical_rate_software.Software_ID _Chemical_rate_software.Software_label _Chemical_rate_software.Method_ID _Chemical_rate_software.Method_label _Chemical_rate_software.Entry_ID _Chemical_rate_software.Chemical_rate_list_ID 1 $NMRPipe . . 17339 1 2 $PIPP . . 17339 1 3 $CAPP . . 17339 1 4 $STAPP . . 17339 1 stop_ loop_ _Chemical_rate.ID _Chemical_rate.Assembly_ID _Chemical_rate.Assembly_atom_ID _Chemical_rate.Entity_assembly_ID _Chemical_rate.Entity_ID _Chemical_rate.Comp_index_ID _Chemical_rate.Seq_ID _Chemical_rate.Comp_ID _Chemical_rate.Atom_ID _Chemical_rate.Atom_type _Chemical_rate.Atom_isotope_number _Chemical_rate.Val_type _Chemical_rate.Val _Chemical_rate.Val_min _Chemical_rate.Val_max _Chemical_rate.Val_err _Chemical_rate.Val_units _Chemical_rate.Resonance_ID _Chemical_rate.Auth_entity_assembly_ID _Chemical_rate.Auth_seq_ID _Chemical_rate.Auth_comp_ID _Chemical_rate.Auth_atom_ID _Chemical_rate.Entry_ID _Chemical_rate.Chemical_rate_list_ID 1 1 . 1 1 . . . . . . kd . 1 5 . s-1 . . . . . 17339 1 stop_ save_ save_binding_data _Binding_value_list.Sf_category binding_data _Binding_value_list.Sf_framecode binding_data _Binding_value_list.Entry_ID 17339 _Binding_value_list.ID 1 _Binding_value_list.Sample_condition_list_ID 1 _Binding_value_list.Sample_condition_list_label $sample_conditions_1 _Binding_value_list.Details . _Binding_value_list.Text_data_format . _Binding_value_list.Text_data . loop_ _Binding_experiment.Experiment_ID _Binding_experiment.Experiment_name _Binding_experiment.Sample_ID _Binding_experiment.Sample_label _Binding_experiment.Sample_state _Binding_experiment.Entry_ID _Binding_experiment.Binding_value_list_ID 1 '2D 1H-15N HSQC' 1 $sample_1 isotropic 17339 1 2 '2D 1H-15N HSQC' 2 $sample_2 isotropic 17339 1 3 '2D 1H-15N HSQC' 3 $sample_3 isotropic 17339 1 stop_ loop_ _Binding_software.Software_ID _Binding_software.Software_label _Binding_software.Method_ID _Binding_software.Method_label _Binding_software.Entry_ID _Binding_software.Binding_value_list_ID 1 $NMRPipe . . 17339 1 2 $PIPP . . 17339 1 3 $CAPP . . 17339 1 4 $STAPP . . 17339 1 stop_ loop_ _Binding_result.ID _Binding_result.Experiment_ID _Binding_result.Assembly_ID _Binding_result.Atm_obs_assembly_atom_ID _Binding_result.Atm_obs_entity_assembly_ID _Binding_result.Atm_obs_entity_ID _Binding_result.Atm_obs_comp_index_ID _Binding_result.Atm_obs_seq_ID _Binding_result.Atm_obs_comp_ID _Binding_result.Atm_obs_atom_ID _Binding_result.Atm_obs_atom_type _Binding_result.Atm_obs_atom_isotope_number _Binding_result.Resonance_ID _Binding_result.Atm_obs_auth_entity_assembly_ID _Binding_result.Atm_obs_auth_seq_ID _Binding_result.Atm_obs_auth_comp_ID _Binding_result.Atm_obs_auth_atom_ID _Binding_result.Expt_observed_param _Binding_result.Val_type _Binding_result.Val _Binding_result.Val_err _Binding_result.Val_units _Binding_result.Entry_ID _Binding_result.Binding_value_list_ID 1 1 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Kamin 50000 . M-1s-1 17339 1 2 2 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Kamin 50000 . M-1s-1 17339 1 3 3 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Kamin 50000 . M-1s-1 17339 1 4 1 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Kamax 2000000 . M-1s-1 17339 1 5 2 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Kamax 2000000 . M-1s-1 17339 1 6 3 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Kamax 2000000 . M-1s-1 17339 1 stop_ loop_ _Binding_partners.Binding_result_ID _Binding_partners.Assembly_ID _Binding_partners.Entity_assembly_ID _Binding_partners.Entity_assembly_name _Binding_partners.Entity_ID _Binding_partners.Entity_label _Binding_partners.Entry_ID _Binding_partners.Binding_value_list_ID 1 1 1 AREA 1 $AREA 17339 1 1 1 2 GATA 2 $GATA 17339 1 2 1 1 AREA 1 $AREA 17339 1 2 1 2 GATA 2 $GATA 17339 1 3 1 1 AREA 1 $AREA 17339 1 3 1 2 GATA 2 $GATA 17339 1 4 1 1 AREA 1 $AREA 17339 1 4 1 2 GATA 2 $GATA 17339 1 5 1 1 AREA 1 $AREA 17339 1 5 1 2 GATA 2 $GATA 17339 1 6 1 1 AREA 1 $AREA 17339 1 6 1 2 GATA 2 $GATA 17339 1 stop_ save_