data_16762 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 16762 _Entry.Title ; Mapping the Ca2+-dependent binding of an invertebrate homolog of protein phosphatase 4 regulatory subunit 2 of the small EF-hand protein, calsensin ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2010-03-03 _Entry.Accession_date 2010-03-03 _Entry.Last_release_date 2010-03-24 _Entry.Original_release_date 2010-03-24 _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 3.0.9.13 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 Deepa Venkitaramani . V. . 16762 2 D. Fulton . B. . 16762 3 Amy Andreotti . H. . 16762 4 Kristen Johansen . M. . 16762 5 Jorgen Johansen . . . 16762 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID binding_constants 1 16762 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID 'binding constants' 1 16762 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 1 . . 2010-03-24 2010-03-03 original author . 16762 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 16762 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 16600403 _Citation.Full_citation . _Citation.Title 'Mapping the Ca2+-dependent binding of an invertebrate homolog of protein phosphatase 4 regulatory subunit 2 of the small EF-hand protein, calsensin' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'Biochim. Biophys. Acta' _Citation.Journal_name_full . _Citation.Journal_volume 1763 _Citation.Journal_issue . _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 322 _Citation.Page_last 329 _Citation.Year 2006 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Deepa Venkitaramani . V. . 16762 1 2 D. Fulton . B. . 16762 1 3 Amy Andreotti . H. . 16762 1 4 Kristen Johansen . M. . 16762 1 5 Jorgen Johansen . . . 16762 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 16762 _Assembly.ID 1 _Assembly.Name 'PP4R2/Calsensin complex' _Assembly.BMRB_code . _Assembly.Number_of_components 2 _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds no _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 PP4R2 1 $PP4R2 A . yes native no no . . . 16762 1 2 Calsensin 2 $Calsensin B . yes native no no . . . 16762 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_PP4R2 _Entity.Sf_category entity _Entity.Sf_framecode PP4R2 _Entity.Entry_ID 16762 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name PP4R2 _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MGSRKEIRDALEEFLKQPED GEVNGILERYLSRVAVTGIT LYPWSKLKPLITRKMNLVVE EFMKECPMESLALNKNVENT SVEDMKKRLNDSIELFNGIP FTIQRLCELLTDQNISKQYR RVDKFMRGIEKNLNVVTTVD PFGNRIVGDPKSASNGVLSA EDDCLLTPTSCTTVKTPPSW FSSLSSVVNSPDSYPNWKQS QDEQQQQEQRDAAISSSRER KKPRSEDDAVDKDCLPPPSK LFRPGDEFVDEAKQPETKDG SDSSVATNSTFTPLLTQLLN GSTPISSSEKEQSVVGSCDS TESSQPSGLLEILTGKLKDP ETNLDASEGTPSVDVGAVGT TSQTDASEVFGAVKSEATQS TQYHVEITEHANKKDVSTAS DKKTDEKIQQEPAEVKRTQE GELESSGVKCQQPSDDPDSL FSF ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 423 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2013-12-02 loop_ _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID no GB ABC88653 . "protein phosphatase 4 regulatory subunit 2 [Haemopis marmorata]" . . . . . 100.00 423 100.00 100.00 0.00e+00 . . . . 16762 1 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 16762 1 2 . GLY . 16762 1 3 . SER . 16762 1 4 . ARG . 16762 1 5 . LYS . 16762 1 6 . GLU . 16762 1 7 . ILE . 16762 1 8 . ARG . 16762 1 9 . ASP . 16762 1 10 . ALA . 16762 1 11 . LEU . 16762 1 12 . GLU . 16762 1 13 . GLU . 16762 1 14 . PHE . 16762 1 15 . LEU . 16762 1 16 . LYS . 16762 1 17 . GLN . 16762 1 18 . PRO . 16762 1 19 . GLU . 16762 1 20 . ASP . 16762 1 21 . GLY . 16762 1 22 . GLU . 16762 1 23 . VAL . 16762 1 24 . ASN . 16762 1 25 . GLY . 16762 1 26 . ILE . 16762 1 27 . LEU . 16762 1 28 . GLU . 16762 1 29 . ARG . 16762 1 30 . TYR . 16762 1 31 . LEU . 16762 1 32 . SER . 16762 1 33 . ARG . 16762 1 34 . VAL . 16762 1 35 . ALA . 16762 1 36 . VAL . 16762 1 37 . THR . 16762 1 38 . GLY . 16762 1 39 . ILE . 16762 1 40 . THR . 16762 1 41 . LEU . 16762 1 42 . TYR . 16762 1 43 . PRO . 16762 1 44 . TRP . 16762 1 45 . SER . 16762 1 46 . LYS . 16762 1 47 . LEU . 16762 1 48 . LYS . 16762 1 49 . PRO . 16762 1 50 . LEU . 16762 1 51 . ILE . 16762 1 52 . THR . 16762 1 53 . ARG . 16762 1 54 . LYS . 16762 1 55 . MET . 16762 1 56 . ASN . 16762 1 57 . LEU . 16762 1 58 . VAL . 16762 1 59 . VAL . 16762 1 60 . GLU . 16762 1 61 . GLU . 16762 1 62 . PHE . 16762 1 63 . MET . 16762 1 64 . LYS . 16762 1 65 . GLU . 16762 1 66 . CYS . 16762 1 67 . PRO . 16762 1 68 . MET . 16762 1 69 . GLU . 16762 1 70 . SER . 16762 1 71 . LEU . 16762 1 72 . ALA . 16762 1 73 . LEU . 16762 1 74 . ASN . 16762 1 75 . LYS . 16762 1 76 . ASN . 16762 1 77 . VAL . 16762 1 78 . GLU . 16762 1 79 . ASN . 16762 1 80 . THR . 16762 1 81 . SER . 16762 1 82 . VAL . 16762 1 83 . GLU . 16762 1 84 . ASP . 16762 1 85 . MET . 16762 1 86 . LYS . 16762 1 87 . LYS . 16762 1 88 . ARG . 16762 1 89 . LEU . 16762 1 90 . ASN . 16762 1 91 . ASP . 16762 1 92 . SER . 16762 1 93 . ILE . 16762 1 94 . GLU . 16762 1 95 . LEU . 16762 1 96 . PHE . 16762 1 97 . ASN . 16762 1 98 . GLY . 16762 1 99 . ILE . 16762 1 100 . PRO . 16762 1 101 . PHE . 16762 1 102 . THR . 16762 1 103 . ILE . 16762 1 104 . GLN . 16762 1 105 . ARG . 16762 1 106 . LEU . 16762 1 107 . CYS . 16762 1 108 . GLU . 16762 1 109 . LEU . 16762 1 110 . LEU . 16762 1 111 . THR . 16762 1 112 . ASP . 16762 1 113 . GLN . 16762 1 114 . ASN . 16762 1 115 . ILE . 16762 1 116 . SER . 16762 1 117 . LYS . 16762 1 118 . GLN . 16762 1 119 . TYR . 16762 1 120 . ARG . 16762 1 121 . ARG . 16762 1 122 . VAL . 16762 1 123 . ASP . 16762 1 124 . LYS . 16762 1 125 . PHE . 16762 1 126 . MET . 16762 1 127 . ARG . 16762 1 128 . GLY . 16762 1 129 . ILE . 16762 1 130 . GLU . 16762 1 131 . LYS . 16762 1 132 . ASN . 16762 1 133 . LEU . 16762 1 134 . ASN . 16762 1 135 . VAL . 16762 1 136 . VAL . 16762 1 137 . THR . 16762 1 138 . THR . 16762 1 139 . VAL . 16762 1 140 . ASP . 16762 1 141 . PRO . 16762 1 142 . PHE . 16762 1 143 . GLY . 16762 1 144 . ASN . 16762 1 145 . ARG . 16762 1 146 . ILE . 16762 1 147 . VAL . 16762 1 148 . GLY . 16762 1 149 . ASP . 16762 1 150 . PRO . 16762 1 151 . LYS . 16762 1 152 . SER . 16762 1 153 . ALA . 16762 1 154 . SER . 16762 1 155 . ASN . 16762 1 156 . GLY . 16762 1 157 . VAL . 16762 1 158 . LEU . 16762 1 159 . SER . 16762 1 160 . ALA . 16762 1 161 . GLU . 16762 1 162 . ASP . 16762 1 163 . ASP . 16762 1 164 . CYS . 16762 1 165 . LEU . 16762 1 166 . LEU . 16762 1 167 . THR . 16762 1 168 . PRO . 16762 1 169 . THR . 16762 1 170 . SER . 16762 1 171 . CYS . 16762 1 172 . THR . 16762 1 173 . THR . 16762 1 174 . VAL . 16762 1 175 . LYS . 16762 1 176 . THR . 16762 1 177 . PRO . 16762 1 178 . PRO . 16762 1 179 . SER . 16762 1 180 . TRP . 16762 1 181 . PHE . 16762 1 182 . SER . 16762 1 183 . SER . 16762 1 184 . LEU . 16762 1 185 . SER . 16762 1 186 . SER . 16762 1 187 . VAL . 16762 1 188 . VAL . 16762 1 189 . ASN . 16762 1 190 . SER . 16762 1 191 . PRO . 16762 1 192 . ASP . 16762 1 193 . SER . 16762 1 194 . TYR . 16762 1 195 . PRO . 16762 1 196 . ASN . 16762 1 197 . TRP . 16762 1 198 . LYS . 16762 1 199 . GLN . 16762 1 200 . SER . 16762 1 201 . GLN . 16762 1 202 . ASP . 16762 1 203 . GLU . 16762 1 204 . GLN . 16762 1 205 . GLN . 16762 1 206 . GLN . 16762 1 207 . GLN . 16762 1 208 . GLU . 16762 1 209 . GLN . 16762 1 210 . ARG . 16762 1 211 . ASP . 16762 1 212 . ALA . 16762 1 213 . ALA . 16762 1 214 . ILE . 16762 1 215 . SER . 16762 1 216 . SER . 16762 1 217 . SER . 16762 1 218 . ARG . 16762 1 219 . GLU . 16762 1 220 . ARG . 16762 1 221 . LYS . 16762 1 222 . LYS . 16762 1 223 . PRO . 16762 1 224 . ARG . 16762 1 225 . SER . 16762 1 226 . GLU . 16762 1 227 . ASP . 16762 1 228 . ASP . 16762 1 229 . ALA . 16762 1 230 . VAL . 16762 1 231 . ASP . 16762 1 232 . LYS . 16762 1 233 . ASP . 16762 1 234 . CYS . 16762 1 235 . LEU . 16762 1 236 . PRO . 16762 1 237 . PRO . 16762 1 238 . PRO . 16762 1 239 . SER . 16762 1 240 . LYS . 16762 1 241 . LEU . 16762 1 242 . PHE . 16762 1 243 . ARG . 16762 1 244 . PRO . 16762 1 245 . GLY . 16762 1 246 . ASP . 16762 1 247 . GLU . 16762 1 248 . PHE . 16762 1 249 . VAL . 16762 1 250 . ASP . 16762 1 251 . GLU . 16762 1 252 . ALA . 16762 1 253 . LYS . 16762 1 254 . GLN . 16762 1 255 . PRO . 16762 1 256 . GLU . 16762 1 257 . THR . 16762 1 258 . LYS . 16762 1 259 . ASP . 16762 1 260 . GLY . 16762 1 261 . SER . 16762 1 262 . ASP . 16762 1 263 . SER . 16762 1 264 . SER . 16762 1 265 . VAL . 16762 1 266 . ALA . 16762 1 267 . THR . 16762 1 268 . ASN . 16762 1 269 . SER . 16762 1 270 . THR . 16762 1 271 . PHE . 16762 1 272 . THR . 16762 1 273 . PRO . 16762 1 274 . LEU . 16762 1 275 . LEU . 16762 1 276 . THR . 16762 1 277 . GLN . 16762 1 278 . LEU . 16762 1 279 . LEU . 16762 1 280 . ASN . 16762 1 281 . GLY . 16762 1 282 . SER . 16762 1 283 . THR . 16762 1 284 . PRO . 16762 1 285 . ILE . 16762 1 286 . SER . 16762 1 287 . SER . 16762 1 288 . SER . 16762 1 289 . GLU . 16762 1 290 . LYS . 16762 1 291 . GLU . 16762 1 292 . GLN . 16762 1 293 . SER . 16762 1 294 . VAL . 16762 1 295 . VAL . 16762 1 296 . GLY . 16762 1 297 . SER . 16762 1 298 . CYS . 16762 1 299 . ASP . 16762 1 300 . SER . 16762 1 301 . THR . 16762 1 302 . GLU . 16762 1 303 . SER . 16762 1 304 . SER . 16762 1 305 . GLN . 16762 1 306 . PRO . 16762 1 307 . SER . 16762 1 308 . GLY . 16762 1 309 . LEU . 16762 1 310 . LEU . 16762 1 311 . GLU . 16762 1 312 . ILE . 16762 1 313 . LEU . 16762 1 314 . THR . 16762 1 315 . GLY . 16762 1 316 . LYS . 16762 1 317 . LEU . 16762 1 318 . LYS . 16762 1 319 . ASP . 16762 1 320 . PRO . 16762 1 321 . GLU . 16762 1 322 . THR . 16762 1 323 . ASN . 16762 1 324 . LEU . 16762 1 325 . ASP . 16762 1 326 . ALA . 16762 1 327 . SER . 16762 1 328 . GLU . 16762 1 329 . GLY . 16762 1 330 . THR . 16762 1 331 . PRO . 16762 1 332 . SER . 16762 1 333 . VAL . 16762 1 334 . ASP . 16762 1 335 . VAL . 16762 1 336 . GLY . 16762 1 337 . ALA . 16762 1 338 . VAL . 16762 1 339 . GLY . 16762 1 340 . THR . 16762 1 341 . THR . 16762 1 342 . SER . 16762 1 343 . GLN . 16762 1 344 . THR . 16762 1 345 . ASP . 16762 1 346 . ALA . 16762 1 347 . SER . 16762 1 348 . GLU . 16762 1 349 . VAL . 16762 1 350 . PHE . 16762 1 351 . GLY . 16762 1 352 . ALA . 16762 1 353 . VAL . 16762 1 354 . LYS . 16762 1 355 . SER . 16762 1 356 . GLU . 16762 1 357 . ALA . 16762 1 358 . THR . 16762 1 359 . GLN . 16762 1 360 . SER . 16762 1 361 . THR . 16762 1 362 . GLN . 16762 1 363 . TYR . 16762 1 364 . HIS . 16762 1 365 . VAL . 16762 1 366 . GLU . 16762 1 367 . ILE . 16762 1 368 . THR . 16762 1 369 . GLU . 16762 1 370 . HIS . 16762 1 371 . ALA . 16762 1 372 . ASN . 16762 1 373 . LYS . 16762 1 374 . LYS . 16762 1 375 . ASP . 16762 1 376 . VAL . 16762 1 377 . SER . 16762 1 378 . THR . 16762 1 379 . ALA . 16762 1 380 . SER . 16762 1 381 . ASP . 16762 1 382 . LYS . 16762 1 383 . LYS . 16762 1 384 . THR . 16762 1 385 . ASP . 16762 1 386 . GLU . 16762 1 387 . LYS . 16762 1 388 . ILE . 16762 1 389 . GLN . 16762 1 390 . GLN . 16762 1 391 . GLU . 16762 1 392 . PRO . 16762 1 393 . ALA . 16762 1 394 . GLU . 16762 1 395 . VAL . 16762 1 396 . LYS . 16762 1 397 . ARG . 16762 1 398 . THR . 16762 1 399 . GLN . 16762 1 400 . GLU . 16762 1 401 . GLY . 16762 1 402 . GLU . 16762 1 403 . LEU . 16762 1 404 . GLU . 16762 1 405 . SER . 16762 1 406 . SER . 16762 1 407 . GLY . 16762 1 408 . VAL . 16762 1 409 . LYS . 16762 1 410 . CYS . 16762 1 411 . GLN . 16762 1 412 . GLN . 16762 1 413 . PRO . 16762 1 414 . SER . 16762 1 415 . ASP . 16762 1 416 . ASP . 16762 1 417 . PRO . 16762 1 418 . ASP . 16762 1 419 . SER . 16762 1 420 . LEU . 16762 1 421 . PHE . 16762 1 422 . SER . 16762 1 423 . PHE . 16762 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 16762 1 . GLY 2 2 16762 1 . SER 3 3 16762 1 . ARG 4 4 16762 1 . LYS 5 5 16762 1 . GLU 6 6 16762 1 . ILE 7 7 16762 1 . ARG 8 8 16762 1 . ASP 9 9 16762 1 . ALA 10 10 16762 1 . LEU 11 11 16762 1 . GLU 12 12 16762 1 . GLU 13 13 16762 1 . PHE 14 14 16762 1 . LEU 15 15 16762 1 . LYS 16 16 16762 1 . GLN 17 17 16762 1 . PRO 18 18 16762 1 . GLU 19 19 16762 1 . ASP 20 20 16762 1 . GLY 21 21 16762 1 . GLU 22 22 16762 1 . VAL 23 23 16762 1 . ASN 24 24 16762 1 . GLY 25 25 16762 1 . ILE 26 26 16762 1 . LEU 27 27 16762 1 . GLU 28 28 16762 1 . ARG 29 29 16762 1 . TYR 30 30 16762 1 . LEU 31 31 16762 1 . SER 32 32 16762 1 . ARG 33 33 16762 1 . VAL 34 34 16762 1 . ALA 35 35 16762 1 . VAL 36 36 16762 1 . THR 37 37 16762 1 . GLY 38 38 16762 1 . ILE 39 39 16762 1 . THR 40 40 16762 1 . LEU 41 41 16762 1 . TYR 42 42 16762 1 . PRO 43 43 16762 1 . TRP 44 44 16762 1 . SER 45 45 16762 1 . LYS 46 46 16762 1 . LEU 47 47 16762 1 . LYS 48 48 16762 1 . PRO 49 49 16762 1 . LEU 50 50 16762 1 . ILE 51 51 16762 1 . THR 52 52 16762 1 . ARG 53 53 16762 1 . LYS 54 54 16762 1 . MET 55 55 16762 1 . ASN 56 56 16762 1 . LEU 57 57 16762 1 . VAL 58 58 16762 1 . VAL 59 59 16762 1 . GLU 60 60 16762 1 . GLU 61 61 16762 1 . PHE 62 62 16762 1 . MET 63 63 16762 1 . LYS 64 64 16762 1 . GLU 65 65 16762 1 . CYS 66 66 16762 1 . PRO 67 67 16762 1 . MET 68 68 16762 1 . GLU 69 69 16762 1 . SER 70 70 16762 1 . LEU 71 71 16762 1 . ALA 72 72 16762 1 . LEU 73 73 16762 1 . ASN 74 74 16762 1 . LYS 75 75 16762 1 . ASN 76 76 16762 1 . VAL 77 77 16762 1 . GLU 78 78 16762 1 . ASN 79 79 16762 1 . THR 80 80 16762 1 . SER 81 81 16762 1 . VAL 82 82 16762 1 . GLU 83 83 16762 1 . ASP 84 84 16762 1 . MET 85 85 16762 1 . LYS 86 86 16762 1 . LYS 87 87 16762 1 . ARG 88 88 16762 1 . LEU 89 89 16762 1 . ASN 90 90 16762 1 . ASP 91 91 16762 1 . SER 92 92 16762 1 . ILE 93 93 16762 1 . GLU 94 94 16762 1 . LEU 95 95 16762 1 . PHE 96 96 16762 1 . ASN 97 97 16762 1 . GLY 98 98 16762 1 . ILE 99 99 16762 1 . PRO 100 100 16762 1 . PHE 101 101 16762 1 . THR 102 102 16762 1 . ILE 103 103 16762 1 . GLN 104 104 16762 1 . ARG 105 105 16762 1 . LEU 106 106 16762 1 . CYS 107 107 16762 1 . GLU 108 108 16762 1 . LEU 109 109 16762 1 . LEU 110 110 16762 1 . THR 111 111 16762 1 . ASP 112 112 16762 1 . GLN 113 113 16762 1 . ASN 114 114 16762 1 . ILE 115 115 16762 1 . SER 116 116 16762 1 . LYS 117 117 16762 1 . GLN 118 118 16762 1 . TYR 119 119 16762 1 . ARG 120 120 16762 1 . ARG 121 121 16762 1 . VAL 122 122 16762 1 . ASP 123 123 16762 1 . LYS 124 124 16762 1 . PHE 125 125 16762 1 . MET 126 126 16762 1 . ARG 127 127 16762 1 . GLY 128 128 16762 1 . ILE 129 129 16762 1 . GLU 130 130 16762 1 . LYS 131 131 16762 1 . ASN 132 132 16762 1 . LEU 133 133 16762 1 . ASN 134 134 16762 1 . VAL 135 135 16762 1 . VAL 136 136 16762 1 . THR 137 137 16762 1 . THR 138 138 16762 1 . VAL 139 139 16762 1 . ASP 140 140 16762 1 . PRO 141 141 16762 1 . PHE 142 142 16762 1 . GLY 143 143 16762 1 . ASN 144 144 16762 1 . ARG 145 145 16762 1 . ILE 146 146 16762 1 . VAL 147 147 16762 1 . GLY 148 148 16762 1 . ASP 149 149 16762 1 . PRO 150 150 16762 1 . LYS 151 151 16762 1 . SER 152 152 16762 1 . ALA 153 153 16762 1 . SER 154 154 16762 1 . ASN 155 155 16762 1 . GLY 156 156 16762 1 . VAL 157 157 16762 1 . LEU 158 158 16762 1 . SER 159 159 16762 1 . ALA 160 160 16762 1 . GLU 161 161 16762 1 . ASP 162 162 16762 1 . ASP 163 163 16762 1 . CYS 164 164 16762 1 . LEU 165 165 16762 1 . LEU 166 166 16762 1 . THR 167 167 16762 1 . PRO 168 168 16762 1 . THR 169 169 16762 1 . SER 170 170 16762 1 . CYS 171 171 16762 1 . THR 172 172 16762 1 . THR 173 173 16762 1 . VAL 174 174 16762 1 . LYS 175 175 16762 1 . THR 176 176 16762 1 . PRO 177 177 16762 1 . PRO 178 178 16762 1 . SER 179 179 16762 1 . TRP 180 180 16762 1 . PHE 181 181 16762 1 . SER 182 182 16762 1 . SER 183 183 16762 1 . LEU 184 184 16762 1 . SER 185 185 16762 1 . SER 186 186 16762 1 . VAL 187 187 16762 1 . VAL 188 188 16762 1 . ASN 189 189 16762 1 . SER 190 190 16762 1 . PRO 191 191 16762 1 . ASP 192 192 16762 1 . SER 193 193 16762 1 . TYR 194 194 16762 1 . PRO 195 195 16762 1 . ASN 196 196 16762 1 . TRP 197 197 16762 1 . LYS 198 198 16762 1 . GLN 199 199 16762 1 . SER 200 200 16762 1 . GLN 201 201 16762 1 . ASP 202 202 16762 1 . GLU 203 203 16762 1 . GLN 204 204 16762 1 . GLN 205 205 16762 1 . GLN 206 206 16762 1 . GLN 207 207 16762 1 . GLU 208 208 16762 1 . GLN 209 209 16762 1 . ARG 210 210 16762 1 . ASP 211 211 16762 1 . ALA 212 212 16762 1 . ALA 213 213 16762 1 . ILE 214 214 16762 1 . SER 215 215 16762 1 . SER 216 216 16762 1 . SER 217 217 16762 1 . ARG 218 218 16762 1 . GLU 219 219 16762 1 . ARG 220 220 16762 1 . LYS 221 221 16762 1 . LYS 222 222 16762 1 . PRO 223 223 16762 1 . ARG 224 224 16762 1 . SER 225 225 16762 1 . GLU 226 226 16762 1 . ASP 227 227 16762 1 . ASP 228 228 16762 1 . ALA 229 229 16762 1 . VAL 230 230 16762 1 . ASP 231 231 16762 1 . LYS 232 232 16762 1 . ASP 233 233 16762 1 . CYS 234 234 16762 1 . LEU 235 235 16762 1 . PRO 236 236 16762 1 . PRO 237 237 16762 1 . PRO 238 238 16762 1 . SER 239 239 16762 1 . LYS 240 240 16762 1 . LEU 241 241 16762 1 . PHE 242 242 16762 1 . ARG 243 243 16762 1 . PRO 244 244 16762 1 . GLY 245 245 16762 1 . ASP 246 246 16762 1 . GLU 247 247 16762 1 . PHE 248 248 16762 1 . VAL 249 249 16762 1 . ASP 250 250 16762 1 . GLU 251 251 16762 1 . ALA 252 252 16762 1 . LYS 253 253 16762 1 . GLN 254 254 16762 1 . PRO 255 255 16762 1 . GLU 256 256 16762 1 . THR 257 257 16762 1 . LYS 258 258 16762 1 . ASP 259 259 16762 1 . GLY 260 260 16762 1 . SER 261 261 16762 1 . ASP 262 262 16762 1 . SER 263 263 16762 1 . SER 264 264 16762 1 . VAL 265 265 16762 1 . ALA 266 266 16762 1 . THR 267 267 16762 1 . ASN 268 268 16762 1 . SER 269 269 16762 1 . THR 270 270 16762 1 . PHE 271 271 16762 1 . THR 272 272 16762 1 . PRO 273 273 16762 1 . LEU 274 274 16762 1 . LEU 275 275 16762 1 . THR 276 276 16762 1 . GLN 277 277 16762 1 . LEU 278 278 16762 1 . LEU 279 279 16762 1 . ASN 280 280 16762 1 . GLY 281 281 16762 1 . SER 282 282 16762 1 . THR 283 283 16762 1 . PRO 284 284 16762 1 . ILE 285 285 16762 1 . SER 286 286 16762 1 . SER 287 287 16762 1 . SER 288 288 16762 1 . GLU 289 289 16762 1 . LYS 290 290 16762 1 . GLU 291 291 16762 1 . GLN 292 292 16762 1 . SER 293 293 16762 1 . VAL 294 294 16762 1 . VAL 295 295 16762 1 . GLY 296 296 16762 1 . SER 297 297 16762 1 . CYS 298 298 16762 1 . ASP 299 299 16762 1 . SER 300 300 16762 1 . THR 301 301 16762 1 . GLU 302 302 16762 1 . SER 303 303 16762 1 . SER 304 304 16762 1 . GLN 305 305 16762 1 . PRO 306 306 16762 1 . SER 307 307 16762 1 . GLY 308 308 16762 1 . LEU 309 309 16762 1 . LEU 310 310 16762 1 . GLU 311 311 16762 1 . ILE 312 312 16762 1 . LEU 313 313 16762 1 . THR 314 314 16762 1 . GLY 315 315 16762 1 . LYS 316 316 16762 1 . LEU 317 317 16762 1 . LYS 318 318 16762 1 . ASP 319 319 16762 1 . PRO 320 320 16762 1 . GLU 321 321 16762 1 . THR 322 322 16762 1 . ASN 323 323 16762 1 . LEU 324 324 16762 1 . ASP 325 325 16762 1 . ALA 326 326 16762 1 . SER 327 327 16762 1 . GLU 328 328 16762 1 . GLY 329 329 16762 1 . THR 330 330 16762 1 . PRO 331 331 16762 1 . SER 332 332 16762 1 . VAL 333 333 16762 1 . ASP 334 334 16762 1 . VAL 335 335 16762 1 . GLY 336 336 16762 1 . ALA 337 337 16762 1 . VAL 338 338 16762 1 . GLY 339 339 16762 1 . THR 340 340 16762 1 . THR 341 341 16762 1 . SER 342 342 16762 1 . GLN 343 343 16762 1 . THR 344 344 16762 1 . ASP 345 345 16762 1 . ALA 346 346 16762 1 . SER 347 347 16762 1 . GLU 348 348 16762 1 . VAL 349 349 16762 1 . PHE 350 350 16762 1 . GLY 351 351 16762 1 . ALA 352 352 16762 1 . VAL 353 353 16762 1 . LYS 354 354 16762 1 . SER 355 355 16762 1 . GLU 356 356 16762 1 . ALA 357 357 16762 1 . THR 358 358 16762 1 . GLN 359 359 16762 1 . SER 360 360 16762 1 . THR 361 361 16762 1 . GLN 362 362 16762 1 . TYR 363 363 16762 1 . HIS 364 364 16762 1 . VAL 365 365 16762 1 . GLU 366 366 16762 1 . ILE 367 367 16762 1 . THR 368 368 16762 1 . GLU 369 369 16762 1 . HIS 370 370 16762 1 . ALA 371 371 16762 1 . ASN 372 372 16762 1 . LYS 373 373 16762 1 . LYS 374 374 16762 1 . ASP 375 375 16762 1 . VAL 376 376 16762 1 . SER 377 377 16762 1 . THR 378 378 16762 1 . ALA 379 379 16762 1 . SER 380 380 16762 1 . ASP 381 381 16762 1 . LYS 382 382 16762 1 . LYS 383 383 16762 1 . THR 384 384 16762 1 . ASP 385 385 16762 1 . GLU 386 386 16762 1 . LYS 387 387 16762 1 . ILE 388 388 16762 1 . GLN 389 389 16762 1 . GLN 390 390 16762 1 . GLU 391 391 16762 1 . PRO 392 392 16762 1 . ALA 393 393 16762 1 . GLU 394 394 16762 1 . VAL 395 395 16762 1 . LYS 396 396 16762 1 . ARG 397 397 16762 1 . THR 398 398 16762 1 . GLN 399 399 16762 1 . GLU 400 400 16762 1 . GLY 401 401 16762 1 . GLU 402 402 16762 1 . LEU 403 403 16762 1 . GLU 404 404 16762 1 . SER 405 405 16762 1 . SER 406 406 16762 1 . GLY 407 407 16762 1 . VAL 408 408 16762 1 . LYS 409 409 16762 1 . CYS 410 410 16762 1 . GLN 411 411 16762 1 . GLN 412 412 16762 1 . PRO 413 413 16762 1 . SER 414 414 16762 1 . ASP 415 415 16762 1 . ASP 416 416 16762 1 . PRO 417 417 16762 1 . ASP 418 418 16762 1 . SER 419 419 16762 1 . LEU 420 420 16762 1 . PHE 421 421 16762 1 . SER 422 422 16762 1 . PHE 423 423 16762 1 stop_ save_ save_Calsensin _Entity.Sf_category entity _Entity.Sf_framecode Calsensin _Entity.Entry_ID 16762 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name Calsensin _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID . _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MACKVKAELEAAFKKLDANG DGYVTALELQTFMVTLDAYK ALSKDKVKEASAKLIKMADK NSDGKISKEEFLNANAELLC QLK ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 83 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not reported' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-01-30 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no BMRB 6519 . Calsensin . . . . . 100.00 83 100.00 100.00 9.39e-49 . . . . 16762 2 2 no PDB 1YX7 . "Nmr Structure Of Calsensin, Energy Minimized Average Structure." . . . . . 100.00 83 100.00 100.00 9.39e-49 . . . . 16762 2 3 no PDB 1YX8 . "Nmr Structure Of Calsensin, 20 Low Energy Structures." . . . . . 100.00 83 100.00 100.00 9.39e-49 . . . . 16762 2 4 no GB AAC46630 . "calsensin [Haemopis marmorata]" . . . . . 100.00 83 100.00 100.00 9.39e-49 . . . . 16762 2 5 no SP Q25088 . "RecName: Full=Calsensin; AltName: Full=LAN3-6 antigen [Haemopis marmorata]" . . . . . 100.00 83 100.00 100.00 9.39e-49 . . . . 16762 2 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 16762 2 2 . ALA . 16762 2 3 . CYS . 16762 2 4 . LYS . 16762 2 5 . VAL . 16762 2 6 . LYS . 16762 2 7 . ALA . 16762 2 8 . GLU . 16762 2 9 . LEU . 16762 2 10 . GLU . 16762 2 11 . ALA . 16762 2 12 . ALA . 16762 2 13 . PHE . 16762 2 14 . LYS . 16762 2 15 . LYS . 16762 2 16 . LEU . 16762 2 17 . ASP . 16762 2 18 . ALA . 16762 2 19 . ASN . 16762 2 20 . GLY . 16762 2 21 . ASP . 16762 2 22 . GLY . 16762 2 23 . TYR . 16762 2 24 . VAL . 16762 2 25 . THR . 16762 2 26 . ALA . 16762 2 27 . LEU . 16762 2 28 . GLU . 16762 2 29 . LEU . 16762 2 30 . GLN . 16762 2 31 . THR . 16762 2 32 . PHE . 16762 2 33 . MET . 16762 2 34 . VAL . 16762 2 35 . THR . 16762 2 36 . LEU . 16762 2 37 . ASP . 16762 2 38 . ALA . 16762 2 39 . TYR . 16762 2 40 . LYS . 16762 2 41 . ALA . 16762 2 42 . LEU . 16762 2 43 . SER . 16762 2 44 . LYS . 16762 2 45 . ASP . 16762 2 46 . LYS . 16762 2 47 . VAL . 16762 2 48 . LYS . 16762 2 49 . GLU . 16762 2 50 . ALA . 16762 2 51 . SER . 16762 2 52 . ALA . 16762 2 53 . LYS . 16762 2 54 . LEU . 16762 2 55 . ILE . 16762 2 56 . LYS . 16762 2 57 . MET . 16762 2 58 . ALA . 16762 2 59 . ASP . 16762 2 60 . LYS . 16762 2 61 . ASN . 16762 2 62 . SER . 16762 2 63 . ASP . 16762 2 64 . GLY . 16762 2 65 . LYS . 16762 2 66 . ILE . 16762 2 67 . SER . 16762 2 68 . LYS . 16762 2 69 . GLU . 16762 2 70 . GLU . 16762 2 71 . PHE . 16762 2 72 . LEU . 16762 2 73 . ASN . 16762 2 74 . ALA . 16762 2 75 . ASN . 16762 2 76 . ALA . 16762 2 77 . GLU . 16762 2 78 . LEU . 16762 2 79 . LEU . 16762 2 80 . CYS . 16762 2 81 . GLN . 16762 2 82 . LEU . 16762 2 83 . LYS . 16762 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 16762 2 . ALA 2 2 16762 2 . CYS 3 3 16762 2 . LYS 4 4 16762 2 . VAL 5 5 16762 2 . LYS 6 6 16762 2 . ALA 7 7 16762 2 . GLU 8 8 16762 2 . LEU 9 9 16762 2 . GLU 10 10 16762 2 . ALA 11 11 16762 2 . ALA 12 12 16762 2 . PHE 13 13 16762 2 . LYS 14 14 16762 2 . LYS 15 15 16762 2 . LEU 16 16 16762 2 . ASP 17 17 16762 2 . ALA 18 18 16762 2 . ASN 19 19 16762 2 . GLY 20 20 16762 2 . ASP 21 21 16762 2 . GLY 22 22 16762 2 . TYR 23 23 16762 2 . VAL 24 24 16762 2 . THR 25 25 16762 2 . ALA 26 26 16762 2 . LEU 27 27 16762 2 . GLU 28 28 16762 2 . LEU 29 29 16762 2 . GLN 30 30 16762 2 . THR 31 31 16762 2 . PHE 32 32 16762 2 . MET 33 33 16762 2 . VAL 34 34 16762 2 . THR 35 35 16762 2 . LEU 36 36 16762 2 . ASP 37 37 16762 2 . ALA 38 38 16762 2 . TYR 39 39 16762 2 . LYS 40 40 16762 2 . ALA 41 41 16762 2 . LEU 42 42 16762 2 . SER 43 43 16762 2 . LYS 44 44 16762 2 . ASP 45 45 16762 2 . LYS 46 46 16762 2 . VAL 47 47 16762 2 . LYS 48 48 16762 2 . GLU 49 49 16762 2 . ALA 50 50 16762 2 . SER 51 51 16762 2 . ALA 52 52 16762 2 . LYS 53 53 16762 2 . LEU 54 54 16762 2 . ILE 55 55 16762 2 . LYS 56 56 16762 2 . MET 57 57 16762 2 . ALA 58 58 16762 2 . ASP 59 59 16762 2 . LYS 60 60 16762 2 . ASN 61 61 16762 2 . SER 62 62 16762 2 . ASP 63 63 16762 2 . GLY 64 64 16762 2 . LYS 65 65 16762 2 . ILE 66 66 16762 2 . SER 67 67 16762 2 . LYS 68 68 16762 2 . GLU 69 69 16762 2 . GLU 70 70 16762 2 . PHE 71 71 16762 2 . LEU 72 72 16762 2 . ASN 73 73 16762 2 . ALA 74 74 16762 2 . ASN 75 75 16762 2 . ALA 76 76 16762 2 . GLU 77 77 16762 2 . LEU 78 78 16762 2 . LEU 79 79 16762 2 . CYS 80 80 16762 2 . GLN 81 81 16762 2 . LEU 82 82 16762 2 . LYS 83 83 16762 2 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 16762 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $PP4R2 . 38567 organism . 'Haemopis marmorata' 'segemented worms' . . Eukaryota Metazoa Hirudo marmorata . . . . . . . . . . . . . . . . . . . . . 16762 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 16762 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $PP4R2 . 'recombinant technology' 'Escherichia coli' . . . Escherichia coli BL21 . . . . . . . . . . . . . . . pGEX4T3 . . . . . . 16762 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 16762 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details 'PP4R2 was titrated into solution' _Sample.Aggregate_sample_number . _Sample.Solvent_system '90% H2O/10% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 Calsensin '[U-100% 15N]' . . 2 $Calsensin . . 0.8 . . mM . . . . 16762 1 2 'sodium phosphate' 'natural abundance' . . . . . . 50 . . mM . . . . 16762 1 3 'sodium chloride' 'natural abundance' . . . . . . 75 . . mM . . . . 16762 1 4 DTT 'natural abundance' . . . . . . 2 . . mM . . . . 16762 1 5 EDTA 'natural abundance' . . . . . . 8 . . mM . . . . 16762 1 6 'sodium azide' 'natural abundance' . . . . . . 0.02 . . % . . . . 16762 1 7 PP4R2 'natural abundance' . . 1 $PP4R2 . . . 0 0.9 mM . . . . 16762 1 8 H2O 'natural abundance' . . . . . . 90 . . % . . . . 16762 1 9 D2O 'natural abundance' . . . . . . 10 . . % . . . . 16762 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 16762 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID pH 6.0 . pH 16762 1 pressure 1 . atm 16762 1 temperature 298 . K 16762 1 stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Software.Sf_category software _Software.Sf_framecode NMRPipe _Software.Entry_ID 16762 _Software.ID 1 _Software.Name NMRPipe _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . 16762 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID processing 16762 1 stop_ save_ save_NMRView _Software.Sf_category software _Software.Sf_framecode NMRView _Software.Entry_ID 16762 _Software.ID 2 _Software.Name NMRView _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Johnson, One Moon Scientific' . . 16762 2 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID assignments 16762 2 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 16762 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details 'Operated at 499.867 MHz' _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model DRX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 500 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 16762 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Bruker DRX . 500 'Operated at 499.867 MHz' . . 16762 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 16762 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-15N HSQC' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 16762 1 stop_ save_ save_binding_data _Binding_value_list.Sf_category binding_data _Binding_value_list.Sf_framecode binding_data _Binding_value_list.Entry_ID 16762 _Binding_value_list.ID 1 _Binding_value_list.Sample_condition_list_ID 1 _Binding_value_list.Sample_condition_list_label $sample_conditions_1 _Binding_value_list.Details . _Binding_value_list.Text_data_format . _Binding_value_list.Text_data . loop_ _Binding_experiment.Experiment_ID _Binding_experiment.Experiment_name _Binding_experiment.Sample_ID _Binding_experiment.Sample_label _Binding_experiment.Sample_state _Binding_experiment.Entry_ID _Binding_experiment.Binding_value_list_ID 1 '2D 1H-15N HSQC' 1 $sample_1 isotropic 16762 1 stop_ loop_ _Binding_software.Software_ID _Binding_software.Software_label _Binding_software.Method_ID _Binding_software.Method_label _Binding_software.Entry_ID _Binding_software.Binding_value_list_ID 1 $NMRPipe . . 16762 1 2 $NMRView . . 16762 1 stop_ loop_ _Binding_result.ID _Binding_result.Experiment_ID _Binding_result.Assembly_ID _Binding_result.Atm_obs_assembly_atom_ID _Binding_result.Atm_obs_entity_assembly_ID _Binding_result.Atm_obs_entity_ID _Binding_result.Atm_obs_comp_index_ID _Binding_result.Atm_obs_seq_ID _Binding_result.Atm_obs_comp_ID _Binding_result.Atm_obs_atom_ID _Binding_result.Atm_obs_atom_type _Binding_result.Atm_obs_atom_isotope_number _Binding_result.Resonance_ID _Binding_result.Atm_obs_auth_entity_assembly_ID _Binding_result.Atm_obs_auth_seq_ID _Binding_result.Atm_obs_auth_comp_ID _Binding_result.Atm_obs_auth_atom_ID _Binding_result.Expt_observed_param _Binding_result.Val_type _Binding_result.Val _Binding_result.Val_err _Binding_result.Val_units _Binding_result.Entry_ID _Binding_result.Binding_value_list_ID 1 1 1 . 1 1 . . . . . . . . . . . 'chemical shifts' Kd 157 70 uM 16762 1 stop_ loop_ _Binding_partners.Binding_result_ID _Binding_partners.Assembly_ID _Binding_partners.Entity_assembly_ID _Binding_partners.Entity_assembly_name _Binding_partners.Entity_ID _Binding_partners.Entity_label _Binding_partners.Entry_ID _Binding_partners.Binding_value_list_ID 1 1 1 PP4R2 1 $PP4R2 16762 1 1 1 2 Calsensin 2 $Calsensin 16762 1 stop_ save_