data_11045 ####################### # Entry information # ####################### save_entry_information _Entry.Sf_category entry_information _Entry.Sf_framecode entry_information _Entry.ID 11045 _Entry.Title ; Mhr1p-bound ssDNA ; _Entry.Type macromolecule _Entry.Version_type original _Entry.Submission_date 2008-05-15 _Entry.Accession_date 2008-05-15 _Entry.Last_release_date . _Entry.Original_release_date . _Entry.Origination author _Entry.NMR_STAR_version 3.1.1.61 _Entry.Original_NMR_STAR_version 3.0.8.120 _Entry.Experimental_method NMR _Entry.Experimental_method_subtype solution _Entry.Details . _Entry.BMRB_internal_directory_name . loop_ _Entry_author.Ordinal _Entry_author.Given_name _Entry_author.Family_name _Entry_author.First_initial _Entry_author.Middle_initials _Entry_author.Family_title _Entry_author.Entry_ID 1 Takehiko Shibata . . . 11045 2 Yutaka Ito . . . 11045 stop_ loop_ _Struct_keywords.Keywords _Struct_keywords.Text _Struct_keywords.Entry_ID ssDNA . 11045 stop_ loop_ _Data_set.Type _Data_set.Count _Data_set.Entry_ID assigned_chemical_shifts 1 11045 stop_ loop_ _Datum.Type _Datum.Count _Datum.Entry_ID '1H chemical shifts' 37 11045 stop_ loop_ _Release.Release_number _Release.Format_type _Release.Format_version _Release.Date _Release.Submission_date _Release.Type _Release.Author _Release.Detail _Release.Entry_ID 3 . . 2012-09-20 2008-05-15 update BMRB 'update sequence to DNA' 11045 2 . . 2010-01-25 2008-05-15 update BMRB 'complete entry citation' 11045 1 . . 2009-10-15 2008-05-15 original author 'original release' 11045 stop_ loop_ _Related_entries.Database_name _Related_entries.Database_accession_code _Related_entries.Relationship _Related_entries.Entry_ID BMRB 11046 'Structure of d(TACG) bound to structurally distinct recombinase hsRad51' 11045 BMRB 11047 'Structure of d(TACG) bound to structurally distinct recombinase ttRecO' 11045 BMRB 11048 'Structure of d(TACG) bound to structurally distinct recombinase ecRecT' 11045 PDB 2RPD 'BMRB Entry Tracking System' 11045 stop_ save_ ############### # Citations # ############### save_entry_citation _Citation.Sf_category citations _Citation.Sf_framecode entry_citation _Citation.Entry_ID 11045 _Citation.ID 1 _Citation.Class 'entry citation' _Citation.CAS_abstract_code . _Citation.MEDLINE_UI_code . _Citation.DOI . _Citation.PubMed_ID 19729448 _Citation.Full_citation . _Citation.Title 'A non-canonical DNA structure enables homologous recombination in various genetic systems.' _Citation.Status published _Citation.Type journal _Citation.Journal_abbrev 'J. Biol. Chem.' _Citation.Journal_name_full 'The Journal of biological chemistry' _Citation.Journal_volume 284 _Citation.Journal_issue 44 _Citation.Journal_ASTM . _Citation.Journal_ISSN . _Citation.Journal_CSD . _Citation.Book_title . _Citation.Book_chapter_title . _Citation.Book_volume . _Citation.Book_series . _Citation.Book_publisher . _Citation.Book_publisher_city . _Citation.Book_ISBN . _Citation.Conference_title . _Citation.Conference_site . _Citation.Conference_state_province . _Citation.Conference_country . _Citation.Conference_start_date . _Citation.Conference_end_date . _Citation.Conference_abstract_number . _Citation.Thesis_institution . _Citation.Thesis_institution_city . _Citation.Thesis_institution_country . _Citation.WWW_URL . _Citation.Page_first 30230 _Citation.Page_last 30239 _Citation.Year 2009 _Citation.Details . loop_ _Citation_author.Ordinal _Citation_author.Given_name _Citation_author.Family_name _Citation_author.First_initial _Citation_author.Middle_initials _Citation_author.Family_title _Citation_author.Entry_ID _Citation_author.Citation_ID 1 Tokiha Masuda . . . 11045 1 2 Yutaka Ito . . . 11045 1 3 Tohru Terada . . . 11045 1 4 Takehiko Shibata . . . 11045 1 5 Tsutomu Mikawa . . . 11045 1 stop_ save_ ############################################# # Molecular system (assembly) description # ############################################# save_assembly _Assembly.Sf_category assembly _Assembly.Sf_framecode assembly _Assembly.Entry_ID 11045 _Assembly.ID 1 _Assembly.Name "5'-D(*DTP*DAP*DCP*DG)-3' 10 structures" _Assembly.BMRB_code . _Assembly.Number_of_components 11 _Assembly.Organic_ligands . _Assembly.Metal_ions . _Assembly.Non_standard_bonds . _Assembly.Ambiguous_conformational_states . _Assembly.Ambiguous_chem_comp_sites . _Assembly.Molecules_in_chemical_exchange . _Assembly.Paramagnetic no _Assembly.Thiol_state . _Assembly.Molecular_mass . _Assembly.Enzyme_commission_number . _Assembly.Details . _Assembly.DB_query_date . _Assembly.DB_query_revised_last_date . loop_ _Entity_assembly.ID _Entity_assembly.Entity_assembly_name _Entity_assembly.Entity_ID _Entity_assembly.Entity_label _Entity_assembly.Asym_ID _Entity_assembly.PDB_chain_ID _Entity_assembly.Experimental_data_reported _Entity_assembly.Physical_state _Entity_assembly.Conformational_isomer _Entity_assembly.Chemical_exchange_state _Entity_assembly.Magnetic_equivalence_group_code _Entity_assembly.Role _Entity_assembly.Details _Entity_assembly.Entry_ID _Entity_assembly.Assembly_ID 1 'DNA (5'-D(*DTP*DAP*DCP*DG)-3') 1' 1 $entity_1 A . yes native no no . . . 11045 1 2 'DNA (5'-D(*DTP*DAP*DCP*DG)-3') 2' 1 $entity_1 B . yes native no no . . . 11045 1 3 'DNA (5'-D(*DTP*DAP*DCP*DG)-3') 3' 1 $entity_1 C . yes native no no . . . 11045 1 4 'DNA (5'-D(*DTP*DAP*DCP*DG)-3') 4' 1 $entity_1 D . yes native no no . . . 11045 1 5 'DNA (5'-D(*DTP*DAP*DCP*DG)-3') 5' 1 $entity_1 E . yes native no no . . . 11045 1 6 'DNA (5'-D(*DTP*DAP*DCP*DG)-3') 6' 1 $entity_1 F . yes native no no . . . 11045 1 7 'DNA (5'-D(*DTP*DAP*DCP*DG)-3') 7' 1 $entity_1 G . yes native no no . . . 11045 1 8 'DNA (5'-D(*DTP*DAP*DCP*DG)-3') 8' 1 $entity_1 H . yes native no no . . . 11045 1 9 'DNA (5'-D(*DTP*DAP*DCP*DG)-3') 9' 1 $entity_1 I . yes native no no . . . 11045 1 10 'DNA (5'-D(*DTP*DAP*DCP*DG)-3') 10' 1 $entity_1 J . yes native no no . . . 11045 1 11 Mhr1p 2 $entity_2 K . no native no no . . ; Mhr1p protein associate/dissociate with nucleic acids quickly enough to measure TRNOE. ; 11045 1 stop_ save_ #################################### # Biological polymers and ligands # #################################### save_entity_1 _Entity.Sf_category entity _Entity.Sf_framecode entity_1 _Entity.Entry_ID 11045 _Entity.ID 1 _Entity.BMRB_code . _Entity.Name 'DNA (5'-D(*DTP*DAP*DCP*DG)-3')' _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polydeoxyribonucleotide _Entity.Polymer_type_details . _Entity.Polymer_strand_ID A-J _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code TACG _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 4 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'not present' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight 1231.30 _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date . loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . DT . 11045 1 2 . DA . 11045 1 3 . DC . 11045 1 4 . DG . 11045 1 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . DT 1 1 11045 1 . DA 2 2 11045 1 . DC 3 3 11045 1 . DG 4 4 11045 1 stop_ save_ save_entity_2 _Entity.Sf_category entity _Entity.Sf_framecode entity_2 _Entity.Entry_ID 11045 _Entity.ID 2 _Entity.BMRB_code . _Entity.Name Mhr1p _Entity.Type polymer _Entity.Polymer_common_type . _Entity.Polymer_type polypeptide(L) _Entity.Polymer_type_details . _Entity.Polymer_strand_ID K _Entity.Polymer_seq_one_letter_code_can . _Entity.Polymer_seq_one_letter_code ; MKVNHSISRFRPASWFEKTK IIPPQVYIFRNLEYGQVLYS QFPNFSQTQVDKLFVRPNWS NRKPSLRRDIWKCMCVVNLQ NYKQSVHLYQNLCRLRYLRD VAQRKESDKLRKKDSNGHVW YSGQYRPTYCQEAVADLRES LLKVFENATPAEKQTVPAKK PSIYWEDPWRMGDKDKHWNY DVFNALGLEHKLIQRVGNIA REESVILKELAKLESHPTEQ TEVSSQ ; _Entity.Target_identifier . _Entity.Polymer_author_defined_seq . _Entity.Polymer_author_seq_details . _Entity.Ambiguous_conformational_states no _Entity.Ambiguous_chem_comp_sites no _Entity.Nstd_monomer no _Entity.Nstd_chirality no _Entity.Nstd_linkage no _Entity.Nonpolymer_comp_ID . _Entity.Nonpolymer_comp_label . _Entity.Number_of_monomers 226 _Entity.Number_of_nonpolymer_components . _Entity.Paramagnetic no _Entity.Thiol_state 'all free' _Entity.Src_method . _Entity.Parent_entity_ID . _Entity.Fragment . _Entity.Mutation . _Entity.EC_number . _Entity.Calc_isoelectric_point . _Entity.Formula_weight . _Entity.Formula_weight_exptl . _Entity.Formula_weight_exptl_meth . _Entity.Details . _Entity.DB_query_date . _Entity.DB_query_revised_last_date 2015-11-26 loop_ _Entity_db_link.Ordinal _Entity_db_link.Author_supplied _Entity_db_link.Database_code _Entity_db_link.Accession_code _Entity_db_link.Entry_mol_code _Entity_db_link.Entry_mol_name _Entity_db_link.Entry_experimental_method _Entity_db_link.Entry_structure_resolution _Entity_db_link.Entry_relation_type _Entity_db_link.Entry_details _Entity_db_link.Chimera_segment_ID _Entity_db_link.Seq_query_to_submitted_percent _Entity_db_link.Seq_subject_length _Entity_db_link.Seq_identity _Entity_db_link.Seq_positive _Entity_db_link.Seq_homology_expectation_val _Entity_db_link.Seq_align_begin _Entity_db_link.Seq_align_end _Entity_db_link.Seq_difference_details _Entity_db_link.Seq_alignment_details _Entity_db_link.Entry_ID _Entity_db_link.Entity_ID 1 no PDB 1VW4 . "Structure Of The Yeast Mitochondrial Large Ribosomal Subunit" . . . . . 100.00 226 100.00 100.00 6.69e-167 . . . . 11045 2 2 no DBJ BAA88081 . "MHR1 [Saccharomyces cerevisiae]" . . . . . 100.00 226 100.00 100.00 6.69e-167 . . . . 11045 2 3 no DBJ GAA22516 . "K7_Mhr1p [Saccharomyces cerevisiae Kyokai no. 7]" . . . . . 100.00 226 100.00 100.00 6.69e-167 . . . . 11045 2 4 no EMBL CAY78797 . "Mhr1p [Saccharomyces cerevisiae EC1118]" . . . . . 100.00 226 98.67 98.67 1.76e-164 . . . . 11045 2 5 no GB AAB64732 . "Ydr296wp [Saccharomyces cerevisiae]" . . . . . 100.00 226 100.00 100.00 6.69e-167 . . . . 11045 2 6 no GB AAS56061 . "YDR296W [Saccharomyces cerevisiae]" . . . . . 100.00 226 100.00 100.00 6.69e-167 . . . . 11045 2 7 no GB AHY75269 . "Mhr1p [Saccharomyces cerevisiae YJM993]" . . . . . 100.00 226 99.12 99.12 5.27e-165 . . . . 11045 2 8 no GB AJP37996 . "Mhr1p [Saccharomyces cerevisiae YJM1078]" . . . . . 100.00 226 99.12 99.12 5.27e-165 . . . . 11045 2 9 no GB AJU58119 . "Mhr1p [Saccharomyces cerevisiae YJM189]" . . . . . 100.00 226 98.67 98.67 3.39e-164 . . . . 11045 2 10 no REF NP_010582 . "Mhr1p [Saccharomyces cerevisiae S288c]" . . . . . 100.00 226 100.00 100.00 6.69e-167 . . . . 11045 2 11 no SP Q06630 . "RecName: Full=Mitochondrial homologous recombination protein 1; AltName: Full=Cross-linked transcription component 1" . . . . . 100.00 226 100.00 100.00 6.69e-167 . . . . 11045 2 12 no TPG DAA12135 . "TPA: Mhr1p [Saccharomyces cerevisiae S288c]" . . . . . 100.00 226 100.00 100.00 6.69e-167 . . . . 11045 2 stop_ loop_ _Entity_comp_index.ID _Entity_comp_index.Auth_seq_ID _Entity_comp_index.Comp_ID _Entity_comp_index.Comp_label _Entity_comp_index.Entry_ID _Entity_comp_index.Entity_ID 1 . MET . 11045 2 2 . LYS . 11045 2 3 . VAL . 11045 2 4 . ASN . 11045 2 5 . HIS . 11045 2 6 . SER . 11045 2 7 . ILE . 11045 2 8 . SER . 11045 2 9 . ARG . 11045 2 10 . PHE . 11045 2 11 . ARG . 11045 2 12 . PRO . 11045 2 13 . ALA . 11045 2 14 . SER . 11045 2 15 . TRP . 11045 2 16 . PHE . 11045 2 17 . GLU . 11045 2 18 . LYS . 11045 2 19 . THR . 11045 2 20 . LYS . 11045 2 21 . ILE . 11045 2 22 . ILE . 11045 2 23 . PRO . 11045 2 24 . PRO . 11045 2 25 . GLN . 11045 2 26 . VAL . 11045 2 27 . TYR . 11045 2 28 . ILE . 11045 2 29 . PHE . 11045 2 30 . ARG . 11045 2 31 . ASN . 11045 2 32 . LEU . 11045 2 33 . GLU . 11045 2 34 . TYR . 11045 2 35 . GLY . 11045 2 36 . GLN . 11045 2 37 . VAL . 11045 2 38 . LEU . 11045 2 39 . TYR . 11045 2 40 . SER . 11045 2 41 . GLN . 11045 2 42 . PHE . 11045 2 43 . PRO . 11045 2 44 . ASN . 11045 2 45 . PHE . 11045 2 46 . SER . 11045 2 47 . GLN . 11045 2 48 . THR . 11045 2 49 . GLN . 11045 2 50 . VAL . 11045 2 51 . ASP . 11045 2 52 . LYS . 11045 2 53 . LEU . 11045 2 54 . PHE . 11045 2 55 . VAL . 11045 2 56 . ARG . 11045 2 57 . PRO . 11045 2 58 . ASN . 11045 2 59 . TRP . 11045 2 60 . SER . 11045 2 61 . ASN . 11045 2 62 . ARG . 11045 2 63 . LYS . 11045 2 64 . PRO . 11045 2 65 . SER . 11045 2 66 . LEU . 11045 2 67 . ARG . 11045 2 68 . ARG . 11045 2 69 . ASP . 11045 2 70 . ILE . 11045 2 71 . TRP . 11045 2 72 . LYS . 11045 2 73 . CYS . 11045 2 74 . MET . 11045 2 75 . CYS . 11045 2 76 . VAL . 11045 2 77 . VAL . 11045 2 78 . ASN . 11045 2 79 . LEU . 11045 2 80 . GLN . 11045 2 81 . ASN . 11045 2 82 . TYR . 11045 2 83 . LYS . 11045 2 84 . GLN . 11045 2 85 . SER . 11045 2 86 . VAL . 11045 2 87 . HIS . 11045 2 88 . LEU . 11045 2 89 . TYR . 11045 2 90 . GLN . 11045 2 91 . ASN . 11045 2 92 . LEU . 11045 2 93 . CYS . 11045 2 94 . ARG . 11045 2 95 . LEU . 11045 2 96 . ARG . 11045 2 97 . TYR . 11045 2 98 . LEU . 11045 2 99 . ARG . 11045 2 100 . ASP . 11045 2 101 . VAL . 11045 2 102 . ALA . 11045 2 103 . GLN . 11045 2 104 . ARG . 11045 2 105 . LYS . 11045 2 106 . GLU . 11045 2 107 . SER . 11045 2 108 . ASP . 11045 2 109 . LYS . 11045 2 110 . LEU . 11045 2 111 . ARG . 11045 2 112 . LYS . 11045 2 113 . LYS . 11045 2 114 . ASP . 11045 2 115 . SER . 11045 2 116 . ASN . 11045 2 117 . GLY . 11045 2 118 . HIS . 11045 2 119 . VAL . 11045 2 120 . TRP . 11045 2 121 . TYR . 11045 2 122 . SER . 11045 2 123 . GLY . 11045 2 124 . GLN . 11045 2 125 . TYR . 11045 2 126 . ARG . 11045 2 127 . PRO . 11045 2 128 . THR . 11045 2 129 . TYR . 11045 2 130 . CYS . 11045 2 131 . GLN . 11045 2 132 . GLU . 11045 2 133 . ALA . 11045 2 134 . VAL . 11045 2 135 . ALA . 11045 2 136 . ASP . 11045 2 137 . LEU . 11045 2 138 . ARG . 11045 2 139 . GLU . 11045 2 140 . SER . 11045 2 141 . LEU . 11045 2 142 . LEU . 11045 2 143 . LYS . 11045 2 144 . VAL . 11045 2 145 . PHE . 11045 2 146 . GLU . 11045 2 147 . ASN . 11045 2 148 . ALA . 11045 2 149 . THR . 11045 2 150 . PRO . 11045 2 151 . ALA . 11045 2 152 . GLU . 11045 2 153 . LYS . 11045 2 154 . GLN . 11045 2 155 . THR . 11045 2 156 . VAL . 11045 2 157 . PRO . 11045 2 158 . ALA . 11045 2 159 . LYS . 11045 2 160 . LYS . 11045 2 161 . PRO . 11045 2 162 . SER . 11045 2 163 . ILE . 11045 2 164 . TYR . 11045 2 165 . TRP . 11045 2 166 . GLU . 11045 2 167 . ASP . 11045 2 168 . PRO . 11045 2 169 . TRP . 11045 2 170 . ARG . 11045 2 171 . MET . 11045 2 172 . GLY . 11045 2 173 . ASP . 11045 2 174 . LYS . 11045 2 175 . ASP . 11045 2 176 . LYS . 11045 2 177 . HIS . 11045 2 178 . TRP . 11045 2 179 . ASN . 11045 2 180 . TYR . 11045 2 181 . ASP . 11045 2 182 . VAL . 11045 2 183 . PHE . 11045 2 184 . ASN . 11045 2 185 . ALA . 11045 2 186 . LEU . 11045 2 187 . GLY . 11045 2 188 . LEU . 11045 2 189 . GLU . 11045 2 190 . HIS . 11045 2 191 . LYS . 11045 2 192 . LEU . 11045 2 193 . ILE . 11045 2 194 . GLN . 11045 2 195 . ARG . 11045 2 196 . VAL . 11045 2 197 . GLY . 11045 2 198 . ASN . 11045 2 199 . ILE . 11045 2 200 . ALA . 11045 2 201 . ARG . 11045 2 202 . GLU . 11045 2 203 . GLU . 11045 2 204 . SER . 11045 2 205 . VAL . 11045 2 206 . ILE . 11045 2 207 . LEU . 11045 2 208 . LYS . 11045 2 209 . GLU . 11045 2 210 . LEU . 11045 2 211 . ALA . 11045 2 212 . LYS . 11045 2 213 . LEU . 11045 2 214 . GLU . 11045 2 215 . SER . 11045 2 216 . HIS . 11045 2 217 . PRO . 11045 2 218 . THR . 11045 2 219 . GLU . 11045 2 220 . GLN . 11045 2 221 . THR . 11045 2 222 . GLU . 11045 2 223 . VAL . 11045 2 224 . SER . 11045 2 225 . SER . 11045 2 226 . GLN . 11045 2 stop_ loop_ _Entity_poly_seq.Hetero _Entity_poly_seq.Mon_ID _Entity_poly_seq.Num _Entity_poly_seq.Comp_index_ID _Entity_poly_seq.Entry_ID _Entity_poly_seq.Entity_ID . MET 1 1 11045 2 . LYS 2 2 11045 2 . VAL 3 3 11045 2 . ASN 4 4 11045 2 . HIS 5 5 11045 2 . SER 6 6 11045 2 . ILE 7 7 11045 2 . SER 8 8 11045 2 . ARG 9 9 11045 2 . PHE 10 10 11045 2 . ARG 11 11 11045 2 . PRO 12 12 11045 2 . ALA 13 13 11045 2 . SER 14 14 11045 2 . TRP 15 15 11045 2 . PHE 16 16 11045 2 . GLU 17 17 11045 2 . LYS 18 18 11045 2 . THR 19 19 11045 2 . LYS 20 20 11045 2 . ILE 21 21 11045 2 . ILE 22 22 11045 2 . PRO 23 23 11045 2 . PRO 24 24 11045 2 . GLN 25 25 11045 2 . VAL 26 26 11045 2 . TYR 27 27 11045 2 . ILE 28 28 11045 2 . PHE 29 29 11045 2 . ARG 30 30 11045 2 . ASN 31 31 11045 2 . LEU 32 32 11045 2 . GLU 33 33 11045 2 . TYR 34 34 11045 2 . GLY 35 35 11045 2 . GLN 36 36 11045 2 . VAL 37 37 11045 2 . LEU 38 38 11045 2 . TYR 39 39 11045 2 . SER 40 40 11045 2 . GLN 41 41 11045 2 . PHE 42 42 11045 2 . PRO 43 43 11045 2 . ASN 44 44 11045 2 . PHE 45 45 11045 2 . SER 46 46 11045 2 . GLN 47 47 11045 2 . THR 48 48 11045 2 . GLN 49 49 11045 2 . VAL 50 50 11045 2 . ASP 51 51 11045 2 . LYS 52 52 11045 2 . LEU 53 53 11045 2 . PHE 54 54 11045 2 . VAL 55 55 11045 2 . ARG 56 56 11045 2 . PRO 57 57 11045 2 . ASN 58 58 11045 2 . TRP 59 59 11045 2 . SER 60 60 11045 2 . ASN 61 61 11045 2 . ARG 62 62 11045 2 . LYS 63 63 11045 2 . PRO 64 64 11045 2 . SER 65 65 11045 2 . LEU 66 66 11045 2 . ARG 67 67 11045 2 . ARG 68 68 11045 2 . ASP 69 69 11045 2 . ILE 70 70 11045 2 . TRP 71 71 11045 2 . LYS 72 72 11045 2 . CYS 73 73 11045 2 . MET 74 74 11045 2 . CYS 75 75 11045 2 . VAL 76 76 11045 2 . VAL 77 77 11045 2 . ASN 78 78 11045 2 . LEU 79 79 11045 2 . GLN 80 80 11045 2 . ASN 81 81 11045 2 . TYR 82 82 11045 2 . LYS 83 83 11045 2 . GLN 84 84 11045 2 . SER 85 85 11045 2 . VAL 86 86 11045 2 . HIS 87 87 11045 2 . LEU 88 88 11045 2 . TYR 89 89 11045 2 . GLN 90 90 11045 2 . ASN 91 91 11045 2 . LEU 92 92 11045 2 . CYS 93 93 11045 2 . ARG 94 94 11045 2 . LEU 95 95 11045 2 . ARG 96 96 11045 2 . TYR 97 97 11045 2 . LEU 98 98 11045 2 . ARG 99 99 11045 2 . ASP 100 100 11045 2 . VAL 101 101 11045 2 . ALA 102 102 11045 2 . GLN 103 103 11045 2 . ARG 104 104 11045 2 . LYS 105 105 11045 2 . GLU 106 106 11045 2 . SER 107 107 11045 2 . ASP 108 108 11045 2 . LYS 109 109 11045 2 . LEU 110 110 11045 2 . ARG 111 111 11045 2 . LYS 112 112 11045 2 . LYS 113 113 11045 2 . ASP 114 114 11045 2 . SER 115 115 11045 2 . ASN 116 116 11045 2 . GLY 117 117 11045 2 . HIS 118 118 11045 2 . VAL 119 119 11045 2 . TRP 120 120 11045 2 . TYR 121 121 11045 2 . SER 122 122 11045 2 . GLY 123 123 11045 2 . GLN 124 124 11045 2 . TYR 125 125 11045 2 . ARG 126 126 11045 2 . PRO 127 127 11045 2 . THR 128 128 11045 2 . TYR 129 129 11045 2 . CYS 130 130 11045 2 . GLN 131 131 11045 2 . GLU 132 132 11045 2 . ALA 133 133 11045 2 . VAL 134 134 11045 2 . ALA 135 135 11045 2 . ASP 136 136 11045 2 . LEU 137 137 11045 2 . ARG 138 138 11045 2 . GLU 139 139 11045 2 . SER 140 140 11045 2 . LEU 141 141 11045 2 . LEU 142 142 11045 2 . LYS 143 143 11045 2 . VAL 144 144 11045 2 . PHE 145 145 11045 2 . GLU 146 146 11045 2 . ASN 147 147 11045 2 . ALA 148 148 11045 2 . THR 149 149 11045 2 . PRO 150 150 11045 2 . ALA 151 151 11045 2 . GLU 152 152 11045 2 . LYS 153 153 11045 2 . GLN 154 154 11045 2 . THR 155 155 11045 2 . VAL 156 156 11045 2 . PRO 157 157 11045 2 . ALA 158 158 11045 2 . LYS 159 159 11045 2 . LYS 160 160 11045 2 . PRO 161 161 11045 2 . SER 162 162 11045 2 . ILE 163 163 11045 2 . TYR 164 164 11045 2 . TRP 165 165 11045 2 . GLU 166 166 11045 2 . ASP 167 167 11045 2 . PRO 168 168 11045 2 . TRP 169 169 11045 2 . ARG 170 170 11045 2 . MET 171 171 11045 2 . GLY 172 172 11045 2 . ASP 173 173 11045 2 . LYS 174 174 11045 2 . ASP 175 175 11045 2 . LYS 176 176 11045 2 . HIS 177 177 11045 2 . TRP 178 178 11045 2 . ASN 179 179 11045 2 . TYR 180 180 11045 2 . ASP 181 181 11045 2 . VAL 182 182 11045 2 . PHE 183 183 11045 2 . ASN 184 184 11045 2 . ALA 185 185 11045 2 . LEU 186 186 11045 2 . GLY 187 187 11045 2 . LEU 188 188 11045 2 . GLU 189 189 11045 2 . HIS 190 190 11045 2 . LYS 191 191 11045 2 . LEU 192 192 11045 2 . ILE 193 193 11045 2 . GLN 194 194 11045 2 . ARG 195 195 11045 2 . VAL 196 196 11045 2 . GLY 197 197 11045 2 . ASN 198 198 11045 2 . ILE 199 199 11045 2 . ALA 200 200 11045 2 . ARG 201 201 11045 2 . GLU 202 202 11045 2 . GLU 203 203 11045 2 . SER 204 204 11045 2 . VAL 205 205 11045 2 . ILE 206 206 11045 2 . LEU 207 207 11045 2 . LYS 208 208 11045 2 . GLU 209 209 11045 2 . LEU 210 210 11045 2 . ALA 211 211 11045 2 . LYS 212 212 11045 2 . LEU 213 213 11045 2 . GLU 214 214 11045 2 . SER 215 215 11045 2 . HIS 216 216 11045 2 . PRO 217 217 11045 2 . THR 218 218 11045 2 . GLU 219 219 11045 2 . GLN 220 220 11045 2 . THR 221 221 11045 2 . GLU 222 222 11045 2 . VAL 223 223 11045 2 . SER 224 224 11045 2 . SER 225 225 11045 2 . GLN 226 226 11045 2 stop_ save_ #################### # Natural source # #################### save_natural_source _Entity_natural_src_list.Sf_category natural_source _Entity_natural_src_list.Sf_framecode natural_source _Entity_natural_src_list.Entry_ID 11045 _Entity_natural_src_list.ID 1 loop_ _Entity_natural_src.ID _Entity_natural_src.Entity_ID _Entity_natural_src.Entity_label _Entity_natural_src.Entity_chimera_segment_ID _Entity_natural_src.NCBI_taxonomy_ID _Entity_natural_src.Type _Entity_natural_src.Common _Entity_natural_src.Organism_name_scientific _Entity_natural_src.Organism_name_common _Entity_natural_src.Organism_acronym _Entity_natural_src.ICTVdb_decimal_code _Entity_natural_src.Superkingdom _Entity_natural_src.Kingdom _Entity_natural_src.Genus _Entity_natural_src.Species _Entity_natural_src.Strain _Entity_natural_src.Variant _Entity_natural_src.Subvariant _Entity_natural_src.Organ _Entity_natural_src.Tissue _Entity_natural_src.Tissue_fraction _Entity_natural_src.Cell_line _Entity_natural_src.Cell_type _Entity_natural_src.ATCC_number _Entity_natural_src.Organelle _Entity_natural_src.Cellular_location _Entity_natural_src.Fragment _Entity_natural_src.Fraction _Entity_natural_src.Secretion _Entity_natural_src.Plasmid _Entity_natural_src.Plasmid_details _Entity_natural_src.Gene_mnemonic _Entity_natural_src.Dev_stage _Entity_natural_src.Details _Entity_natural_src.Citation_ID _Entity_natural_src.Citation_label _Entity_natural_src.Entry_ID _Entity_natural_src.Entity_natural_src_list_ID 1 1 $entity_1 . . 'no natural source' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11045 1 2 2 $entity_2 . 4932 organism . 'Saccharomyces cerevisiae' 'baker's yeast' . . Eukaryota Fungi Saccharomyces cerevisiae . . . . . . . . . . . . . . . . . . . . . 11045 1 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Entity_experimental_src_list.Sf_category experimental_source _Entity_experimental_src_list.Sf_framecode experimental_source _Entity_experimental_src_list.Entry_ID 11045 _Entity_experimental_src_list.ID 1 loop_ _Entity_experimental_src.ID _Entity_experimental_src.Entity_ID _Entity_experimental_src.Entity_label _Entity_experimental_src.Entity_chimera_segment_ID _Entity_experimental_src.Production_method _Entity_experimental_src.Host_org_scientific_name _Entity_experimental_src.Host_org_name_common _Entity_experimental_src.Host_org_details _Entity_experimental_src.Host_org_NCBI_taxonomy_ID _Entity_experimental_src.Host_org_genus _Entity_experimental_src.Host_org_species _Entity_experimental_src.Host_org_strain _Entity_experimental_src.Host_org_variant _Entity_experimental_src.Host_org_subvariant _Entity_experimental_src.Host_org_organ _Entity_experimental_src.Host_org_tissue _Entity_experimental_src.Host_org_tissue_fraction _Entity_experimental_src.Host_org_cell_line _Entity_experimental_src.Host_org_cell_type _Entity_experimental_src.Host_org_cellular_location _Entity_experimental_src.Host_org_organelle _Entity_experimental_src.Host_org_gene _Entity_experimental_src.Host_org_culture_collection _Entity_experimental_src.Host_org_ATCC_number _Entity_experimental_src.Vector_type _Entity_experimental_src.PDBview_host_org_vector_name _Entity_experimental_src.PDBview_plasmid_name _Entity_experimental_src.Vector_name _Entity_experimental_src.Vector_details _Entity_experimental_src.Vendor_name _Entity_experimental_src.Host_org_dev_stage _Entity_experimental_src.Details _Entity_experimental_src.Citation_ID _Entity_experimental_src.Citation_label _Entity_experimental_src.Entry_ID _Entity_experimental_src.Entity_experimental_src_list_ID 1 1 $entity_1 . 'obtained from a vendor' . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11045 1 2 2 $entity_2 . 'recombinant technology' 'Escherichia coli BL21(DE3)' . . 469008 Escherichia coli BL21(DE3) . . . . . . . . . . . . plasmid . . pET14b . . . . . . 11045 1 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Sample.Sf_category sample _Sample.Sf_framecode sample_1 _Sample.Entry_ID 11045 _Sample.ID 1 _Sample.Type solution _Sample.Sub_type . _Sample.Details . _Sample.Aggregate_sample_number . _Sample.Solvent_system '100% D2O' _Sample.Preparation_date . _Sample.Preparation_expiration_date . _Sample.Polycrystallization_protocol . _Sample.Single_crystal_protocol . _Sample.Crystal_grow_apparatus . _Sample.Crystal_grow_atmosphere . _Sample.Crystal_grow_details . _Sample.Crystal_grow_method . _Sample.Crystal_grow_method_cit_ID . _Sample.Crystal_grow_pH . _Sample.Crystal_grow_pH_range . _Sample.Crystal_grow_pressure . _Sample.Crystal_grow_pressure_esd . _Sample.Crystal_grow_seeding . _Sample.Crystal_grow_seeding_cit_ID . _Sample.Crystal_grow_temp . _Sample.Crystal_grow_temp_details . _Sample.Crystal_grow_temp_esd . _Sample.Crystal_grow_time . _Sample.Oriented_sample_prep_protocol . _Sample.Lyophilization_cryo_protectant . _Sample.Storage_protocol . loop_ _Sample_component.ID _Sample_component.Mol_common_name _Sample_component.Isotopic_labeling _Sample_component.Assembly_ID _Sample_component.Assembly_label _Sample_component.Entity_ID _Sample_component.Entity_label _Sample_component.Product_ID _Sample_component.Type _Sample_component.Concentration_val _Sample_component.Concentration_val_min _Sample_component.Concentration_val_max _Sample_component.Concentration_val_units _Sample_component.Concentration_val_err _Sample_component.Vendor _Sample_component.Vendor_product_name _Sample_component.Vendor_product_code _Sample_component.Entry_ID _Sample_component.Sample_ID 1 'DNA (5'-D(*DTP*DAP*DCP*DG)-3')' 'natural abundance' . . 1 $entity_1 . DNA 0.5 . . mM . . . . 11045 1 2 Mhr1p 'natural abundance' . . 2 $entity_2 . protein 0.05 . . mM . . . . 11045 1 3 MgCl2 'natural abundance' . . . . . salt 6.7 . . mM . . . . 11045 1 4 NaCl 'natural abundance' . . . . . salt 50 . . mM . . . . 11045 1 5 D2O . . . . . . solvent 100 . . % . . . . 11045 1 stop_ save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Sample_condition_list.Sf_category sample_conditions _Sample_condition_list.Sf_framecode sample_conditions_1 _Sample_condition_list.Entry_ID 11045 _Sample_condition_list.ID 1 _Sample_condition_list.Details . loop_ _Sample_condition_variable.Type _Sample_condition_variable.Val _Sample_condition_variable.Val_err _Sample_condition_variable.Val_units _Sample_condition_variable.Entry_ID _Sample_condition_variable.Sample_condition_list_ID 'ionic strength' 50 . mM 11045 1 pH 7.1 . pH 11045 1 pressure 1 . atm 11045 1 temperature 298 . K 11045 1 stop_ save_ ############################ # Computer software used # ############################ save_X-PLOR_NIH _Software.Sf_category software _Software.Sf_framecode X-PLOR_NIH _Software.Entry_ID 11045 _Software.ID 1 _Software.Name 'X-PLOR NIH' _Software.Version . _Software.Details . loop_ _Vendor.Name _Vendor.Address _Vendor.Electronic_address _Vendor.Entry_ID _Vendor.Software_ID 'Schwieters, Kuszewski, Tjandra and Clore' . . 11045 1 stop_ loop_ _Task.Task _Task.Entry_ID _Task.Software_ID processing 11045 1 stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _NMR_spectrometer.Sf_category NMR_spectrometer _NMR_spectrometer.Sf_framecode spectrometer_1 _NMR_spectrometer.Entry_ID 11045 _NMR_spectrometer.ID 1 _NMR_spectrometer.Details . _NMR_spectrometer.Manufacturer Bruker _NMR_spectrometer.Model DRX _NMR_spectrometer.Serial_number . _NMR_spectrometer.Field_strength 600 save_ save_NMR_spectrometer_list _NMR_spectrometer_list.Sf_category NMR_spectrometer_list _NMR_spectrometer_list.Sf_framecode NMR_spectrometer_list _NMR_spectrometer_list.Entry_ID 11045 _NMR_spectrometer_list.ID 1 loop_ _NMR_spectrometer_view.ID _NMR_spectrometer_view.Name _NMR_spectrometer_view.Manufacturer _NMR_spectrometer_view.Model _NMR_spectrometer_view.Serial_number _NMR_spectrometer_view.Field_strength _NMR_spectrometer_view.Details _NMR_spectrometer_view.Citation_ID _NMR_spectrometer_view.Citation_label _NMR_spectrometer_view.Entry_ID _NMR_spectrometer_view.NMR_spectrometer_list_ID 1 spectrometer_1 Bruker DRX . 600 . . . 11045 1 stop_ save_ ############################# # NMR applied experiments # ############################# save_experiment_list _Experiment_list.Sf_category experiment_list _Experiment_list.Sf_framecode experiment_list _Experiment_list.Entry_ID 11045 _Experiment_list.ID 1 _Experiment_list.Details . loop_ _Experiment.ID _Experiment.Name _Experiment.Raw_data_flag _Experiment.NMR_spec_expt_ID _Experiment.NMR_spec_expt_label _Experiment.MS_expt_ID _Experiment.MS_expt_label _Experiment.SAXS_expt_ID _Experiment.SAXS_expt_label _Experiment.FRET_expt_ID _Experiment.FRET_expt_label _Experiment.EMR_expt_ID _Experiment.EMR_expt_label _Experiment.Sample_ID _Experiment.Sample_label _Experiment.Sample_state _Experiment.Sample_volume _Experiment.Sample_volume_units _Experiment.Sample_condition_list_ID _Experiment.Sample_condition_list_label _Experiment.Sample_spinning_rate _Experiment.Sample_angle _Experiment.NMR_tube_type _Experiment.NMR_spectrometer_ID _Experiment.NMR_spectrometer_label _Experiment.NMR_spectrometer_probe_ID _Experiment.NMR_spectrometer_probe_label _Experiment.NMR_spectral_processing_ID _Experiment.NMR_spectral_processing_label _Experiment.Mass_spectrometer_ID _Experiment.Mass_spectrometer_label _Experiment.Xray_instrument_ID _Experiment.Xray_instrument_label _Experiment.Fluorescence_instrument_ID _Experiment.Fluorescence_instrument_label _Experiment.EMR_instrument_ID _Experiment.EMR_instrument_label _Experiment.Chromatographic_system_ID _Experiment.Chromatographic_system_label _Experiment.Chromatographic_column_ID _Experiment.Chromatographic_column_label _Experiment.Entry_ID _Experiment.Experiment_list_ID 1 '2D 1H-1H NOESY' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 11045 1 2 '2D 1H-1H transferred NOESY' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 11045 1 3 '2D 1H-1H DQF-COSY' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 11045 1 4 '2D 1H-1H TOCSY' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 11045 1 5 '2D 1H-1H ROESY' no . . . . . . . . . . 1 $sample_1 isotropic . . 1 $sample_conditions_1 . . . 1 $spectrometer_1 . . . . . . . . . . . . . . . . 11045 1 stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Chem_shift_reference.Sf_category chem_shift_reference _Chem_shift_reference.Sf_framecode chemical_shift_reference_1 _Chem_shift_reference.Entry_ID 11045 _Chem_shift_reference.ID 1 _Chem_shift_reference.Details . loop_ _Chem_shift_ref.Atom_type _Chem_shift_ref.Atom_isotope_number _Chem_shift_ref.Mol_common_name _Chem_shift_ref.Atom_group _Chem_shift_ref.Concentration_val _Chem_shift_ref.Concentration_units _Chem_shift_ref.Solvent _Chem_shift_ref.Rank _Chem_shift_ref.Chem_shift_units _Chem_shift_ref.Chem_shift_val _Chem_shift_ref.Ref_method _Chem_shift_ref.Ref_type _Chem_shift_ref.Indirect_shift_ratio _Chem_shift_ref.External_ref_loc _Chem_shift_ref.External_ref_sample_geometry _Chem_shift_ref.External_ref_axis _Chem_shift_ref.Indirect_shift_ratio_cit_ID _Chem_shift_ref.Indirect_shift_ratio_cit_label _Chem_shift_ref.Ref_correction_type _Chem_shift_ref.Correction_val _Chem_shift_ref.Correction_val_cit_ID _Chem_shift_ref.Correction_val_cit_label _Chem_shift_ref.Entry_ID _Chem_shift_ref.Chem_shift_reference_ID H 1 DSS 'methyl protons' . . . . ppm 0.0 . indirect 1.0 . . . . . . . . . 11045 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Assigned_chem_shift_list.Sf_category assigned_chemical_shifts _Assigned_chem_shift_list.Sf_framecode assigned_chem_shift_list_1 _Assigned_chem_shift_list.Entry_ID 11045 _Assigned_chem_shift_list.ID 1 _Assigned_chem_shift_list.Sample_condition_list_ID 1 _Assigned_chem_shift_list.Sample_condition_list_label $sample_conditions_1 _Assigned_chem_shift_list.Chem_shift_reference_ID 1 _Assigned_chem_shift_list.Chem_shift_reference_label $chemical_shift_reference_1 _Assigned_chem_shift_list.Chem_shift_1H_err . _Assigned_chem_shift_list.Chem_shift_13C_err . _Assigned_chem_shift_list.Chem_shift_15N_err . _Assigned_chem_shift_list.Chem_shift_31P_err . _Assigned_chem_shift_list.Chem_shift_2H_err . _Assigned_chem_shift_list.Chem_shift_19F_err . _Assigned_chem_shift_list.Error_derivation_method . _Assigned_chem_shift_list.Details . _Assigned_chem_shift_list.Text_data_format . _Assigned_chem_shift_list.Text_data . loop_ _Chem_shift_experiment.Experiment_ID _Chem_shift_experiment.Experiment_name _Chem_shift_experiment.Sample_ID _Chem_shift_experiment.Sample_label _Chem_shift_experiment.Sample_state _Chem_shift_experiment.Entry_ID _Chem_shift_experiment.Assigned_chem_shift_list_ID 1 '2D 1H-1H NOESY' 1 $sample_1 isotropic 11045 1 2 '2D 1H-1H transferred NOESY' 1 $sample_1 isotropic 11045 1 3 '2D 1H-1H DQF-COSY' 1 $sample_1 isotropic 11045 1 4 '2D 1H-1H TOCSY' 1 $sample_1 isotropic 11045 1 5 '2D 1H-1H ROESY' 1 $sample_1 isotropic 11045 1 stop_ loop_ _Atom_chem_shift.ID _Atom_chem_shift.Assembly_atom_ID _Atom_chem_shift.Entity_assembly_ID _Atom_chem_shift.Entity_ID _Atom_chem_shift.Comp_index_ID _Atom_chem_shift.Seq_ID _Atom_chem_shift.Comp_ID _Atom_chem_shift.Atom_ID _Atom_chem_shift.Atom_type _Atom_chem_shift.Atom_isotope_number _Atom_chem_shift.Val _Atom_chem_shift.Val_err _Atom_chem_shift.Assign_fig_of_merit _Atom_chem_shift.Ambiguity_code _Atom_chem_shift.Occupancy _Atom_chem_shift.Resonance_ID _Atom_chem_shift.Auth_entity_assembly_ID _Atom_chem_shift.Auth_asym_ID _Atom_chem_shift.Auth_seq_ID _Atom_chem_shift.Auth_comp_ID _Atom_chem_shift.Auth_atom_ID _Atom_chem_shift.Details _Atom_chem_shift.Entry_ID _Atom_chem_shift.Assigned_chem_shift_list_ID 1 . 1 1 1 1 DT H1' H 1 5.93 0.01 . 1 . . . . 1 T H1' . 11045 1 2 . 1 1 1 1 DT H2' H 1 1.64 0.01 . 2 . . . . 1 T H2' . 11045 1 3 . 1 1 1 1 DT H2'' H 1 2.18 0.01 . 2 . . . . 1 T H2'' . 11045 1 4 . 1 1 1 1 DT H3' H 1 4.60 0.01 . 1 . . . . 1 T H3' . 11045 1 5 . 1 1 1 1 DT H4' H 1 4.00 0.01 . 1 . . . . 1 T H4' . 11045 1 6 . 1 1 1 1 DT H5' H 1 3.63 0.01 . 2 . . . . 1 T H5' . 11045 1 7 . 1 1 1 1 DT H5'' H 1 3.63 0.01 . 2 . . . . 1 T H5'' . 11045 1 8 . 1 1 1 1 DT H6 H 1 7.31 0.01 . 1 . . . . 1 T H6 . 11045 1 9 . 1 1 1 1 DT H71 H 1 1.81 0.01 . 1 . . . . 1 T H7 . 11045 1 10 . 1 1 1 1 DT H72 H 1 1.81 0.01 . 1 . . . . 1 T H7 . 11045 1 11 . 1 1 1 1 DT H73 H 1 1.81 0.01 . 1 . . . . 1 T H7 . 11045 1 12 . 1 1 2 2 DA H1' H 1 6.26 0.01 . 1 . . . . 2 A H1' . 11045 1 13 . 1 1 2 2 DA H2 H 1 8.07 0.01 . 1 . . . . 2 A H2 . 11045 1 14 . 1 1 2 2 DA H2' H 1 2.80 0.01 . 1 . . . . 2 A H2' . 11045 1 15 . 1 1 2 2 DA H2'' H 1 2.70 0.01 . 1 . . . . 2 A H2'' . 11045 1 16 . 1 1 2 2 DA H3' H 1 4.98 0.01 . 1 . . . . 2 A H3' . 11045 1 17 . 1 1 2 2 DA H4' H 1 4.37 0.01 . 1 . . . . 2 A H4' . 11045 1 18 . 1 1 2 2 DA H5' H 1 4.02 0.01 . 2 . . . . 2 A H5' . 11045 1 19 . 1 1 2 2 DA H5'' H 1 4.02 0.01 . 2 . . . . 2 A H5'' . 11045 1 20 . 1 1 2 2 DA H8 H 1 8.33 0.01 . 1 . . . . 2 A H8 . 11045 1 21 . 1 1 3 3 DC H1' H 1 6.06 0.01 . 1 . . . . 3 C H1' . 11045 1 22 . 1 1 3 3 DC H2' H 1 1.92 0.01 . 1 . . . . 3 C H2' . 11045 1 23 . 1 1 3 3 DC H2'' H 1 2.35 0.01 . 1 . . . . 3 C H2'' . 11045 1 24 . 1 1 3 3 DC H3' H 1 4.75 0.01 . 1 . . . . 3 C H3' . 11045 1 25 . 1 1 3 3 DC H4' H 1 4.18 0.01 . 1 . . . . 3 C H4' . 11045 1 26 . 1 1 3 3 DC H5 H 1 5.79 0.01 . 1 . . . . 3 C H5 . 11045 1 27 . 1 1 3 3 DC H5' H 1 4.04 0.01 . 2 . . . . 3 C H5' . 11045 1 28 . 1 1 3 3 DC H5'' H 1 4.04 0.01 . 2 . . . . 3 C H5'' . 11045 1 29 . 1 1 3 3 DC H6 H 1 7.53 0.01 . 1 . . . . 3 C H6 . 11045 1 30 . 1 1 4 4 DG H1' H 1 6.15 0.01 . 1 . . . . 4 G H1' . 11045 1 31 . 1 1 4 4 DG H2' H 1 2.71 0.01 . 1 . . . . 4 G H2' . 11045 1 32 . 1 1 4 4 DG H2'' H 1 2.44 0.01 . 1 . . . . 4 G H2'' . 11045 1 33 . 1 1 4 4 DG H3' H 1 4.68 0.01 . 1 . . . . 4 G H3' . 11045 1 34 . 1 1 4 4 DG H4' H 1 4.16 0.01 . 1 . . . . 4 G H4' . 11045 1 35 . 1 1 4 4 DG H5' H 1 4.07 0.01 . 2 . . . . 4 G H5' . 11045 1 36 . 1 1 4 4 DG H5'' H 1 4.07 0.01 . 2 . . . . 4 G H5'' . 11045 1 37 . 1 1 4 4 DG H8 H 1 7.95 0.01 . 1 . . . . 4 G H8 . 11045 1 stop_ save_