data_6569 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Measles virus P protein (amino acids 457-507) bound to the Measles virus N protein (amino acids 477-505) ; _BMRB_accession_number 6569 _BMRB_flat_file_name bmr6569.str _Entry_type original _Submission_date 2005-03-24 _Accession_date 2005-03-28 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kingston Richard L. . 2 Hamel Damon J. . 3 Gay Leslie S. . 4 Dahlquist Frederick W. . 5 Matthews Brian W. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 49 "15N chemical shifts" 49 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2005-06-03 original author . stop_ _Original_release_date 2005-06-03 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structural basis for the attachment of a paramyxoviral polymerase to its template' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 15159535 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kingston Richard L . 2 Hamel Damon J . 3 Gay Leslie S . 4 Dahlquist Frederick W . 5 Matthews Brian W . stop_ _Journal_abbreviation 'Proc. Natl. Acad. Sci. U.S.A.' _Journal_volume 101 _Journal_issue 22 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 8301 _Page_last 8306 _Year 2004 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Measles virus P protein (amino acids 457-507) bound to the measles virus N protein (amino acids 477-505)' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Measles virus P protein (amino acids 457-507)' $Measles_virus_P_protein_(amino_acids_457-507) 'Measles virus N protein (amino acids 477-505)' $Measles_virus_N_protein_(amino_acids_477-505) stop_ _System_molecular_weight 9277 _System_physical_state native _System_oligomer_state 'protein-protein complex' _System_paramagnetic no _System_thiol_state 'not present' loop_ _Biological_function 'Measles virus polymerase-nucleocapsid interaction' stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Measles_virus_P_protein_(amino_acids_457-507) _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'Measles virus P protein (amino acids 457-507)' _Molecular_mass 5879 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 51 _Mol_residue_sequence ; GGASRSVIRSIIKSSRLEED RKRYLMTLLDDIKGANDLAK FHQMLMKIIMK ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 457 GLY 2 458 GLY 3 459 ALA 4 460 SER 5 461 ARG 6 462 SER 7 463 VAL 8 464 ILE 9 465 ARG 10 466 SER 11 467 ILE 12 468 ILE 13 469 LYS 14 470 SER 15 471 SER 16 472 ARG 17 473 LEU 18 474 GLU 19 475 GLU 20 476 ASP 21 477 ARG 22 478 LYS 23 479 ARG 24 480 TYR 25 481 LEU 26 482 MET 27 483 THR 28 484 LEU 29 485 LEU 30 486 ASP 31 487 ASP 32 488 ILE 33 489 LYS 34 490 GLY 35 491 ALA 36 492 ASN 37 493 ASP 38 494 LEU 39 495 ALA 40 496 LYS 41 497 PHE 42 498 HIS 43 499 GLN 44 500 MET 45 501 LEU 46 502 MET 47 503 LYS 48 504 ILE 49 505 ILE 50 506 MET 51 507 LYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-28 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 6566 Measles_virus_N_protein_(amino_acids_477-505) 100.00 32 100.00 100.00 3.10e-12 PDB 1T6O "Nucleocapsid-Binding Domain Of The Measles Virus P Protein (Amino Acids 457-507) In Complex With Amino Acids 486-505 Of The Mea" 62.50 20 100.00 100.00 6.27e-03 DBJ BAA00801 "nucleocapsid protein [Measles virus]" 90.63 151 100.00 100.00 5.59e-09 DBJ BAA00806 "nucleocapsid protein [Measles virus]" 90.63 151 100.00 100.00 5.59e-09 EMBL CAA76595 "nucleocapsid protein [Measles virus]" 90.63 128 100.00 100.00 4.88e-09 EMBL CBW39466 "nucleoprotein [Measles virus]" 90.63 151 100.00 100.00 5.37e-09 GB AAA18998 "nucleoprotein [Measles virus]" 90.63 525 100.00 100.00 3.94e-08 GB AAA56646 "nucleoprotein [Measles virus]" 90.63 525 100.00 100.00 3.91e-08 GB AAA56658 "nucleoprotein [Measles virus]" 90.63 525 100.00 100.00 3.94e-08 GB AAB65448 "nucleocapsid protein, partial [Measles virus]" 90.63 151 100.00 100.00 5.37e-09 GB AAB65449 "nucleocapsid protein, partial [Measles virus]" 90.63 151 100.00 100.00 5.37e-09 SP B8PZP3 "RecName: Full=Nucleoprotein; AltName: Full=Nucleocapsid protein; Short=NP; Short=Protein N [Measles virus strain Schwarz]" 90.63 525 100.00 100.00 3.94e-08 SP Q77M43 "RecName: Full=Nucleoprotein; AltName: Full=Nucleocapsid protein; Short=NP; Short=Protein N [Measles virus strain Moraten]" 90.63 525 100.00 100.00 3.94e-08 stop_ save_ save_Measles_virus_N_protein_(amino_acids_477-505) _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'Measles virus N protein (amino acids 477-505)' _Molecular_mass 3398 _Mol_thiol_state 'not present' _Details . _Residue_count 32 _Mol_residue_sequence ; GTTATESSQDPQDSRRSADA LLRLQAMAGISY ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 475 GLY 2 476 THR 3 477 THR 4 478 ALA 5 479 THR 6 480 GLU 7 481 SER 8 482 SER 9 483 GLN 10 484 ASP 11 485 PRO 12 486 GLN 13 487 ASP 14 488 SER 15 489 ARG 16 490 ARG 17 491 SER 18 492 ALA 19 493 ASP 20 494 ALA 21 495 LEU 22 496 LEU 23 497 ARG 24 498 LEU 25 499 GLN 26 500 ALA 27 501 MET 28 502 ALA 29 503 GLY 30 504 ILE 31 505 SER 32 506 TYR stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Measles_virus_P_protein_(amino_acids_457-507) 'Measles virus, Moraten vaccine strain' 11234 Viruses 'Not applicable' Morbillivirus 'Measles virus' $Measles_virus_N_protein_(amino_acids_477-505) 'Measles virus, Moraten vaccine strain' 11234 Viruses 'Not applicable' Morbillivirus 'Measles virus' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Measles_virus_P_protein_(amino_acids_457-507) 'recombinant technology' 'Escherichia coli' . . . . $Measles_virus_N_protein_(amino_acids_477-505) 'recombinant technology' 'Escherichia coli' . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Measles_virus_P_protein_(amino_acids_457-507) 0.23 mM [U-15N] $Measles_virus_N_protein_(amino_acids_477-505) 0.68 mM unlabeled 'sodium phosphate' 9 mM . 'sodium chloride' 90 mM . 'sodium azide' 0.01 %(w/v) . stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . _Details . save_ save_Sparky _Saveframe_category software _Name SPARKY _Version . _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_600MHz_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model Inova _Field_strength '600 MHz' _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_HNCACB_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_H(CCO)NH_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D H(CCO)NH' _Sample_label $sample_1 save_ save_3D_C(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D C(CO)NH' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_1H15N_HSQC_(example) _Saveframe_category NMR_applied_experiment _Experiment_name . _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.7 0.1 pH pressure 1.0 0.1 atm temperature 293 1.0 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_referencing _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS C 13 'methyl protons' ppm 0.0 . indirect . . . 0.251449530 $entry_citation $entry_citation DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0 $entry_citation $entry_citation DSS N 15 'methyl protons' ppm 0.0 . indirect . . . 0.101329118 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $NMRPipe $Sparky stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $conditions_1 _Chem_shift_reference_set_label $chemical_shift_referencing _Mol_system_component_name 'Measles virus P protein (amino acids 457-507)' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 459 3 ALA H H 8.389 . 1 2 459 3 ALA N N 122.124 . 1 3 460 4 SER H H 8.735 . 1 4 460 4 SER N N 118.438 . 1 5 461 5 ARG H H 9.110 . 1 6 461 5 ARG N N 122.491 . 1 7 462 6 SER H H 8.362 . 1 8 462 6 SER N N 112.547 . 1 9 463 7 VAL H H 7.554 . 1 10 463 7 VAL N N 125.176 . 1 11 464 8 ILE H H 8.212 . 1 12 464 8 ILE N N 120.868 . 1 13 465 9 ARG H H 8.507 . 1 14 465 9 ARG N N 119.149 . 1 15 466 10 SER H H 7.802 . 1 16 466 10 SER N N 114.337 . 1 17 467 11 ILE H H 8.242 . 1 18 467 11 ILE N N 124.898 . 1 19 468 12 ILE H H 8.068 . 1 20 468 12 ILE N N 118.650 . 1 21 469 13 LYS H H 8.522 . 1 22 469 13 LYS N N 119.579 . 1 23 470 14 SER H H 8.070 . 1 24 470 14 SER N N 111.711 . 1 25 471 15 SER H H 7.441 . 1 26 471 15 SER N N 118.091 . 1 27 472 16 ARG H H 8.971 . 1 28 472 16 ARG N N 117.260 . 1 29 473 17 LEU H H 7.006 . 1 30 473 17 LEU N N 119.309 . 1 31 474 18 GLU H H 8.981 . 1 32 474 18 GLU N N 119.822 . 1 33 475 19 GLU H H 9.068 . 1 34 475 19 GLU N N 123.079 . 1 35 476 20 ASP H H 9.069 . 1 36 476 20 ASP N N 116.852 . 1 37 477 21 ARG H H 7.350 . 1 38 477 21 ARG N N 121.404 . 1 39 478 22 LYS H H 8.200 . 1 40 478 22 LYS N N 119.335 . 1 41 479 23 ARG H H 8.145 . 1 42 479 23 ARG N N 117.074 . 1 43 480 24 TYR H H 7.880 . 1 44 480 24 TYR N N 120.865 . 1 45 481 25 LEU H H 8.459 . 1 46 481 25 LEU N N 119.028 . 1 47 482 26 MET H H 8.319 . 1 48 482 26 MET N N 117.340 . 1 49 483 27 THR H H 7.938 . 1 50 483 27 THR N N 115.556 . 1 51 484 28 LEU H H 7.809 . 1 52 484 28 LEU N N 121.891 . 1 53 485 29 LEU H H 7.982 . 1 54 485 29 LEU N N 118.739 . 1 55 486 30 ASP H H 7.489 . 1 56 486 30 ASP N N 117.266 . 1 57 487 31 ASP H H 7.444 . 1 58 487 31 ASP N N 118.701 . 1 59 488 32 ILE H H 7.457 . 1 60 488 32 ILE N N 121.836 . 1 61 489 33 LYS H H 9.458 . 1 62 489 33 LYS N N 128.528 . 1 63 490 34 GLY H H 8.640 . 1 64 490 34 GLY N N 113.213 . 1 65 491 35 ALA H H 8.130 . 1 66 491 35 ALA N N 123.917 . 1 67 492 36 ASN H H 7.831 . 1 68 492 36 ASN N N 115.665 . 1 69 493 37 ASP H H 8.965 . 1 70 493 37 ASP N N 123.255 . 1 71 494 38 LEU H H 8.086 . 1 72 494 38 LEU N N 122.223 . 1 73 495 39 ALA H H 7.871 . 1 74 495 39 ALA N N 121.069 . 1 75 496 40 LYS H H 7.871 . 1 76 496 40 LYS N N 119.637 . 1 77 497 41 PHE H H 8.694 . 1 78 497 41 PHE N N 121.461 . 1 79 498 42 HIS H H 8.774 . 1 80 498 42 HIS N N 118.192 . 1 81 499 43 GLN H H 8.119 . 1 82 499 43 GLN N N 116.738 . 1 83 500 44 MET H H 7.919 . 1 84 500 44 MET N N 117.793 . 1 85 501 45 LEU H H 8.799 . 1 86 501 45 LEU N N 120.623 . 1 87 502 46 MET H H 7.813 . 1 88 502 46 MET N N 113.918 . 1 89 503 47 LYS H H 7.237 . 1 90 503 47 LYS N N 117.214 . 1 91 504 48 ILE H H 7.617 . 1 92 504 48 ILE N N 118.094 . 1 93 505 49 ILE H H 7.944 . 1 94 505 49 ILE N N 114.864 . 1 95 506 50 MET H H 7.652 . 1 96 506 50 MET N N 119.411 . 1 97 507 51 LYS H H 7.495 . 1 98 507 51 LYS N N 126.511 . 1 stop_ save_