data_6485 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; A novel RNA pentaloop fold involved in targeting ADAR2 ; _BMRB_accession_number 6485 _BMRB_flat_file_name bmr6485.str _Entry_type new _Submission_date 2005-02-03 _Accession_date 2005-02-04 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Stefl Richard . . 2 Allain Frederic . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 221 "13C chemical shifts" 184 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2011-04-20 update author 'Update chemical shifts' 2005-05-26 original author 'Original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'A novel RNA pentaloop fold involved in targeting ADAR2' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 15840813 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Stefl R. . . 2 Allain F. H. . stop_ _Journal_abbreviation RNA _Journal_volume 11 _Journal_issue 5 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 592 _Page_last 597 _Year 2005 _Details . save_ ################################## # Molecular system description # ################################## save_GluR-B_R_G_RNA _Saveframe_category molecular_system _Mol_system_name 'GluR-B R/G RNA' _Abbreviation_common 'GluR-B R/G RNA' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'GluR-B R/G RNA' $R_G_stem-loop stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'not present' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_R_G_stem-loop _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common 'GluR-B R/G stem-loop RNA' _Abbreviation_common 'R/G stem-loop' _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 27 _Mol_residue_sequence ; GGUAACAAUAUGCUAAAUGU UGUUACC ; loop_ _Residue_seq_code _Residue_label 1 G 2 G 3 U 4 A 5 A 6 C 7 A 8 A 9 U 10 A 11 U 12 G 13 C 14 U 15 A 16 A 17 A 18 U 19 G 20 U 21 U 22 G 23 U 24 U 25 A 26 C 27 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $R_G_stem-loop 9606 Human Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $R_G_stem-loop 'cell free synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $R_G_stem-loop . mM 0.5 2 '[U-13C; U-15N]' stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 900 _Details . save_ save_NMR_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label $sample_1 save_ save_2D_TOCSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _Sample_label $sample_1 save_ save_13C-HSQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name 13C-HSQC _Sample_label $sample_1 save_ save_3D_13-separated_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 13-separated NOESY' _Sample_label $sample_1 save_ save_and_3D_HCCH-TOCSY_5 _Saveframe_category NMR_applied_experiment _Experiment_name 'and 3D HCCH-TOCSY' _Sample_label $sample_1 save_ save_NMR_spec_expt__0_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_3 _Saveframe_category NMR_applied_experiment _Experiment_name 13C-HSQC _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 13-separated NOESY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_5 _Saveframe_category NMR_applied_experiment _Experiment_name 'and 3D HCCH-TOCSY' _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_condition_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6 0.1 pH temperature 303 1 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio _Indirect_shift_ratio_citation_label _Correction_value_citation_label DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0 $entry_citation $entry_citation DSS C 13 'methyl protons' ppm 0.0 . indirect . . . 0.251449530 $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $condition_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'GluR-B R/G RNA' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 G H8 H 8.14 . 1 2 . 1 G C8 C 139.3 . 1 3 . 1 G H1' H 5.81 . 1 4 . 1 G H2' H 4.95 . 1 5 . 1 G H3' H 4.7 . 1 6 . 1 G H4' H 4.55 . 1 7 . 1 G H5' H 4.24 . 2 8 . 1 G H5'' H 4.42 . 2 9 . 1 G C1' C 92.1 . 1 10 . 1 G C2' C 75.1 . 1 11 . 1 G C3' C 74.5 . 1 12 . 1 G C4' C 83.6 . 1 13 . 1 G C5' C 67.2 . 1 14 . 2 G H1 H 13.32 . 1 15 . 2 G H8 H 7.53 . 1 16 . 2 G C8 C 137 . 1 17 . 2 G H1' H 5.89 . 1 18 . 2 G H2' H 4.53 . 1 19 . 2 G H3' H 4.53 . 1 20 . 2 G H4' H 4.54 . 1 21 . 2 G H5' H 4.22 . 2 22 . 2 G H5'' H 4.50 . 2 23 . 2 G C1' C 93.3 . 1 24 . 2 G C2' C 75.5 . 1 25 . 2 G C3' C 73.1 . 1 26 . 2 G C4' C 82.7 . 1 27 . 2 G C5' C 66.1 . 1 28 . 3 U H3 H 13.52 . 1 29 . 3 U H5 H 5.14 . 1 30 . 3 U H6 H 7.75 . 1 31 . 3 U C5 C 103.2 . 1 32 . 3 U C6 C 141.4 . 1 33 . 3 U H1' H 5.52 . 1 34 . 3 U H2' H 4.56 . 1 35 . 3 U H3' H 4.58 . 1 36 . 3 U H4' H 4.45 . 1 37 . 3 U H5' H 4.11 . 2 38 . 3 U H5'' H 4.58 . 2 39 . 3 U C1' C 93.8 . 1 40 . 3 U C2' C 75.5 . 1 41 . 3 U C3' C 72.4 . 1 42 . 3 U C4' C 82.3 . 1 43 . 3 U C5' C 64.6 . 1 44 . 4 A H2 H 6.40 . 1 45 . 4 A H8 H 8.06 . 1 46 . 4 A C2 C 152.3 . 1 47 . 4 A C8 C 139.7 . 1 48 . 4 A H1' H 5.9 . 1 49 . 4 A H2' H 4.54 . 1 50 . 4 A H3' H 4.71 . 1 51 . 4 A H4' H 4.53 . 1 52 . 4 A H5' H 4.19 . 2 53 . 4 A H5'' H 4.53 . 2 54 . 4 A C1' C 92.9 . 1 55 . 4 A C2' C 75.8 . 1 56 . 4 A C3' C 73.4 . 1 57 . 4 A C4' C 82.3 . 1 58 . 4 A C5' C 65.9 . 1 59 . 5 A H2 H 7.55 . 1 60 . 5 A H8 H 7.83 . 1 61 . 5 A C2 C 153.9 . 1 62 . 5 A C8 C 139.1 . 1 63 . 5 A H1' H 5.81 . 1 64 . 5 A H2' H 4.41 . 1 65 . 5 A H3' H 4.56 . 1 66 . 5 A H4' H 4.47 . 1 67 . 5 A H5' H 4.14 . 2 68 . 5 A H5'' H 4.58 . 2 69 . 5 A C1' C 92.7 . 1 70 . 5 A C2' C 75.7 . 1 71 . 5 A C3' C 72.8 . 1 72 . 5 A C4' C 82.2 . 1 73 . 5 A C5' C 65.3 . 1 74 . 6 C H5 H 5.15 . 1 75 . 6 C H6 H 7.38 . 1 76 . 6 C C5 C 97.7 . 1 77 . 6 C C6 C 140.2 . 1 78 . 6 C H1' H 5.25 . 1 79 . 6 C H2' H 4.31 . 1 80 . 6 C H3' H 4.41 . 1 81 . 6 C H4' H 4.35 . 1 82 . 6 C H5' H 4.04 . 2 83 . 6 C H5'' H 4.48 . 2 84 . 6 C C1' C 93.6 . 1 85 . 6 C C2' C 75.6 . 1 86 . 6 C C3' C 72.4 . 1 87 . 6 C C4' C 81.8 . 1 88 . 6 C C5' C 64.8 . 1 89 . 7 A H2 H 6.74 . 1 90 . 7 A H8 H 7.90 . 1 91 . 7 A C2 C 152.4 . 1 92 . 7 A C8 C 139.6 . 1 93 . 7 A H1' H 5.83 . 1 94 . 7 A H2' H 4.64 . 1 95 . 7 A H3' H 4.41 . 1 96 . 7 A H4' H 4.48 . 1 97 . 7 A H5' H 4.12 . 2 98 . 7 A H5'' H 4.51 . 2 99 . 7 A C1' C 93.0 . 1 100 . 7 A C2' C 75.8 . 1 101 . 7 A C3' C 72.7 . 1 102 . 7 A C4' C 82.2 . 1 103 . 7 A C5' C 65.4 . 1 104 . 8 A H2 H 7.70 . 1 105 . 8 A H8 H 7.64 . 1 106 . 8 A C2 C 154 . 1 107 . 8 A C8 C 139.1 . 1 108 . 8 A H1' H 5.83 . 1 109 . 8 A H2' H 4.55 . 1 110 . 8 A H3' H 4.68 . 1 111 . 8 A H4' H 4.48 . 1 112 . 8 A H5' H 4.10 . 2 113 . 8 A H5'' H 4.53 . 2 114 . 8 A C1' C 93.2 . 1 115 . 8 A C2' C 75.6 . 1 116 . 8 A C3' C 73.2 . 1 117 . 8 A C4' C 82.2 . 1 118 . 8 A C5' C 65.3 . 1 119 . 9 U H3 H 11.58 . 1 120 . 9 U H5 H 5.32 . 1 121 . 9 U H6 H 7.47 . 1 122 . 9 U C5 C 104.4 . 1 123 . 9 U C6 C 140.2 . 1 124 . 9 U H1' H 5.22 . 1 125 . 9 U H2' H 4.10 . 1 126 . 9 U H3' H 4.51 . 1 127 . 9 U H4' H 4.38 . 1 128 . 9 U H5' H 4.06 . 2 129 . 9 U H5'' H 4.47 . 2 130 . 9 U C1' C 93.3 . 1 131 . 9 U C2' C 75.8 . 1 132 . 9 U C3' C 72.8 . 1 133 . 9 U C4' C 82.6 . 1 134 . 9 U C5' C 64.5 . 1 135 . 10 A H2 H 7.37 . 1 136 . 10 A H8 H 8.28 . 1 137 . 10 A C2 C 153.6 . 1 138 . 10 A C8 C 140.9 . 1 139 . 10 A H1' H 5.94 . 1 140 . 10 A H2' H 4.47 . 1 141 . 10 A H3' H 4.7 . 1 142 . 10 A H4' H 4.45 . 1 143 . 10 A H5' H 4.15 . 2 144 . 10 A H5'' H 4.52 . 2 145 . 10 A C1' C 92.6 . 1 146 . 10 A C2' C 75.8 . 1 147 . 10 A C3' C 73.3 . 1 148 . 10 A C4' C 82.6 . 1 149 . 10 A C5' C 65.7 . 1 150 . 11 U H5 H 4.97 . 1 151 . 11 U H6 H 7.34 . 1 152 . 11 U C5 C 103.7 . 1 153 . 11 U C6 C 140.7 . 1 154 . 11 U H1' H 5.49 . 1 155 . 11 U H2' H 4.37 . 1 156 . 11 U H3' H 4.34 . 1 157 . 11 U H4' H 4.37 . 1 158 . 11 U H5' H 4.09 . 2 159 . 11 U H5'' H 4.44 . 2 160 . 11 U C1' C 93.3 . 1 161 . 11 U C2' C 75.8 . 1 162 . 11 U C3' C 72.9 . 1 163 . 11 U C4' C 82.5 . 1 164 . 11 U C5' C 65.3 . 1 165 . 12 G H8 H 7.79 . 1 166 . 12 G C8 C 137.4 . 1 167 . 12 G H1' H 5.55 . 1 168 . 12 G H2' H 4.30 . 1 169 . 12 G H3' H 4.59 . 1 170 . 12 G H4' H 4.42 . 1 171 . 12 G H5' H 4.10 . 2 172 . 12 G H5'' H 4.34 . 2 173 . 12 G C1' C 91.9 . 1 174 . 12 G C2' C 76.0 . 1 175 . 12 G C3' C 74.5 . 1 176 . 12 G C4' C 83.5 . 1 177 . 12 G C5' C 66.0 . 1 178 . 13 C H5 H 5.62 . 1 179 . 13 C H6 H 7.52 . 1 180 . 13 C C5 C 97.9 . 1 181 . 13 C C6 C 143.7 . 1 182 . 13 C H1' H 5.15 . 1 183 . 13 C H2' H 4.32 . 1 184 . 13 C H3' H 4.17 . 1 185 . 13 C H4' H 4.10 . 1 186 . 13 C H5' H 3.97 . 2 187 . 13 C H5'' H 4.16 . 2 188 . 13 C C1' C 92.4 . 1 189 . 13 C C2' C 75.4 . 1 190 . 13 C C3' C 75.4 . 1 191 . 13 C C4' C 84.1 . 1 192 . 13 C C5' C 66.7 . 1 193 . 14 U H5 H 5.76 . 1 194 . 14 U H6 H 7.70 . 1 195 . 14 U C5 C 105.3 . 1 196 . 14 U C6 C 143.7 . 1 197 . 14 U H1' H 5.66 . 1 198 . 14 U H2' H 4.29 . 1 199 . 14 U H3' H 4.41 . 1 200 . 14 U H4' H 4.18 . 1 201 . 14 U H5' H 3.88 . 2 202 . 14 U H5'' H 3.94 . 2 203 . 14 U C1' C 91.3 . 1 204 . 14 U C2' C 75.8 . 1 205 . 14 U C3' C 75.5 . 1 206 . 14 U C4' C 84.6 . 1 207 . 14 U C5' C 66.5 . 1 208 . 15 A H2 H 8.01 . 1 209 . 15 A H8 H 8.08 . 1 210 . 15 A C2 C 155 . 1 211 . 15 A C8 C 141.9 . 1 212 . 15 A H1' H 5.84 . 1 213 . 15 A H2' H 4.75 . 1 214 . 15 A H3' H 4.41 . 1 215 . 15 A H4' H 4.38 . 1 216 . 15 A H5' H 3.99 . 2 217 . 15 A H5'' H 4.15 . 2 218 . 15 A C1' C 91.7 . 1 219 . 15 A C2' C 75.7 . 1 220 . 15 A C3' C 72.7 . 1 221 . 15 A C4' C 84.2 . 1 222 . 15 A C5' C 67.1 . 1 223 . 16 A H2 H 8.10 . 1 224 . 16 A H8 H 8.08 . 1 225 . 16 A C2 C 155.2 . 1 226 . 16 A C8 C 141.7 . 1 227 . 16 A H1' H 5.59 . 1 228 . 16 A H2' H 4.74 . 1 229 . 16 A H3' H 4.39 . 1 230 . 16 A H4' H 4.54 . 1 231 . 16 A H5' H 4.31 . 2 232 . 16 A H5'' H 4.37 . 2 233 . 16 A C1' C 91.6 . 1 234 . 16 A C2' C 75.9 . 1 235 . 16 A C3' C 74.3 . 1 236 . 16 A C4' C 83.8 . 1 237 . 16 A C5' C 67.9 . 1 238 . 17 A H2 H 7.87 . 1 239 . 17 A H8 H 8.15 . 1 240 . 17 A C2 C 154.1 . 1 241 . 17 A C8 C 140.7 . 1 242 . 17 A H1' H 5.01 . 1 243 . 17 A H2' H 4.41 . 1 244 . 17 A H3' H 4.4 . 1 245 . 17 A H4' H 4.39 . 1 246 . 17 A H5' H 4.28 . 2 247 . 17 A H5'' H 4.33 . 2 248 . 17 A C1' C 93.0 . 1 249 . 17 A C2' C 75.5 . 1 250 . 17 A C3' C 74.2 . 1 251 . 17 A C4' C 83.0 . 1 252 . 17 A C5' C 67.6 . 1 253 . 18 U H3 H 13.62 . 1 254 . 18 U H5 H 5.11 . 1 255 . 18 U H6 H 7.53 . 1 256 . 18 U C5 C 103.3 . 1 257 . 18 U C6 C 141.8 . 1 258 . 18 U H1' H 5.57 . 1 259 . 18 U H2' H 4.52 . 1 260 . 18 U H3' H 4.47 . 1 261 . 18 U H4' H 4.41 . 1 262 . 18 U H5' H 4.08 . 2 263 . 18 U H5'' H 4.43 . 2 264 . 18 U C1' C 92.82 . 1 265 . 18 U C2' C 75.8 . 1 266 . 18 U C3' C 73.3 . 1 267 . 18 U C4' C 82.3 . 1 268 . 18 U C5' C 65.1 . 1 269 . 19 G H1 H 11 . 1 270 . 19 G H8 H 7.67 . 1 271 . 19 G C8 C 137.4 . 1 272 . 19 G H1' H 5.77 . 1 273 . 19 G H2' H 4.63 . 1 274 . 19 G H3' H 4.25 . 1 275 . 19 G H4' H 4.52 . 1 276 . 19 G H5' H 4.15 . 2 277 . 19 G H5'' H 4.39 . 2 278 . 19 G C1' C 93.7 . 1 279 . 19 G C2' C 75.3 . 1 280 . 19 G C3' C 73.6 . 1 281 . 19 G C4' C 82.5 . 1 282 . 19 G C5' C 67.2 . 1 283 . 20 U H3 H 14.2 . 1 284 . 20 U H5 H 5.16 . 1 285 . 20 U H6 H 7.62 . 1 286 . 20 U C5 C 103.2 . 1 287 . 20 U C6 C 141.1 . 1 288 . 20 U H1' H 5.42 . 1 289 . 20 U H2' H 4.35 . 1 290 . 20 U H3' H 4.46 . 1 291 . 20 U H4' H 4.41 . 1 292 . 20 U H5' H 4.08 . 2 293 . 20 U H5'' H 4.5 . 2 294 . 20 U C1' C 94.1 . 1 295 . 20 U C2' C 75.5 . 1 296 . 20 U C3' C 72.1 . 1 297 . 20 U C4' C 82.3 . 1 298 . 20 U C5' C 64.1 . 1 299 . 21 U H3 H 13.21 . 1 300 . 21 U H5 H 5.53 . 1 301 . 21 U H6 H 7.88 . 1 302 . 21 U C5 C 103.9 . 1 303 . 21 U C6 C 142.0 . 1 304 . 21 U H1' H 5.59 . 1 305 . 21 U H2' H 4.64 . 1 306 . 21 U H3' H 4.57 . 1 307 . 21 U H4' H 4.46 . 1 308 . 21 U H5' H 4.12 . 2 309 . 21 U H5'' H 4.51 . 2 310 . 21 U C1' C 93.6 . 1 311 . 21 U C2' C 75.5 . 1 312 . 21 U C3' C 73.0 . 1 313 . 21 U C4' C 82.3 . 1 314 . 21 U C5' C 65.0 . 1 315 . 22 G H1 H 12.48 . 1 316 . 22 G H8 H 7.69 . 1 317 . 22 G C8 C 136.7 . 1 318 . 22 G H1' H 5.71 . 1 319 . 22 G H2' H 4.48 . 1 320 . 22 G H3' H 4.51 . 1 321 . 22 G H4' H 4.52 . 1 322 . 22 G H5' H 4.16 . 2 323 . 22 G H5'' H 4.48 . 2 324 . 22 G C1' C 93.1 . 1 325 . 22 G C2' C 75.3 . 1 326 . 22 G C3' C 73.4 . 1 327 . 22 G C4' C 82.5 . 1 328 . 22 G C5' C 66.2 . 1 329 . 23 U H3 H 14.25 . 1 330 . 23 U H5 H 5.02 . 1 331 . 23 U H6 H 7.77 . 1 332 . 23 U C5 C 102.6 . 1 333 . 23 U C6 C 142 . 1 334 . 23 U H1' H 5.46 . 1 335 . 23 U H2' H 4.41 . 1 336 . 23 U H3' H 4.46 . 1 337 . 23 U H4' H 4.44 . 1 338 . 23 U H5' H 4.10 . 2 339 . 23 U H5'' H 4.57 . 2 340 . 23 U C1' C 94.0 . 1 341 . 23 U C2' C 75.3 . 1 342 . 23 U C3' C 72.2 . 1 343 . 23 U C4' C 82.3 . 1 344 . 23 U C5' C 64.5 . 1 345 . 24 U H3 H 13.12 . 1 346 . 24 U H5 H 5.59 . 1 347 . 24 U H6 H 7.99 . 1 348 . 24 U C5 C 103.7 . 1 349 . 24 U C6 C 142.3 . 1 350 . 24 U H1' H 5.59 . 1 351 . 24 U H2' H 4.48 . 1 352 . 24 U H3' H 4.61 . 1 353 . 24 U H4' H 4.45 . 1 354 . 24 U H5' H 4.13 . 2 355 . 24 U H5'' H 4.54 . 2 356 . 24 U C1' C 93.6 . 1 357 . 24 U C2' C 75.5 . 1 358 . 24 U C3' C 72.6 . 1 359 . 24 U C4' C 82.2 . 1 360 . 24 U C5' C 64.8 . 1 361 . 25 A H2 H 7.04 . 1 362 . 25 A H8 H 8.19 . 1 363 . 25 A C2 C 153.5 . 1 364 . 25 A C8 C 139.8 . 1 365 . 25 A H1' H 5.96 . 1 366 . 25 A H2' H 4.46 . 1 367 . 25 A H3' H 4.68 . 1 368 . 25 A H4' H 4.49 . 1 369 . 25 A H5' H 4.16 . 2 370 . 25 A H5'' H 4.58 . 2 371 . 25 A C1' C 93.0 . 1 372 . 25 A C2' C 75.8 . 1 373 . 25 A C3' C 72.9 . 1 374 . 25 A C4' C 82.1 . 1 375 . 25 A C5' C 65.2 . 1 376 . 26 C H5 H 5.18 . 1 377 . 26 C H6 H 7.51 . 1 378 . 26 C C5 C 97.1 . 1 379 . 26 C C6 C 140.9 . 1 380 . 26 C H1' H 5.33 . 1 381 . 26 C H2' H 4.04 . 1 382 . 26 C H3' H 4.33 . 1 383 . 26 C H4' H 4.33 . 1 384 . 26 C H5' H 4.01 . 2 385 . 26 C H5'' H 4.49 . 2 386 . 26 C C1' C 94.0 . 1 387 . 26 C C2' C 75.9 . 1 388 . 26 C C3' C 72.3 . 1 389 . 26 C C4' C 81.9 . 1 390 . 26 C C5' C 64.7 . 1 391 . 27 C H5 H 5.4 . 1 392 . 27 C H6 H 7.59 . 1 393 . 27 C C5 C 98.2 . 1 394 . 27 C C6 C 141.9 . 1 395 . 27 C H1' H 5.71 . 1 396 . 27 C H2' H 3.97 . 1 397 . 27 C H3' H 4.14 . 1 398 . 27 C H4' H 4.12 . 1 399 . 27 C H5' H 3.99 . 2 400 . 27 C H5'' H 4.42 . 2 401 . 27 C C1' C 93.0 . 1 402 . 27 C C2' C 77.7 . 1 403 . 27 C C3' C 70.0 . 1 404 . 27 C C4' C 83.6 . 1 405 . 27 C C5' C 65.3 . 1 stop_ save_