data_5919 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; The solution structure of the loop E region of the 5S rRNA from spinach chloroplasts ; _BMRB_accession_number 5919 _BMRB_flat_file_name bmr5919.str _Entry_type original _Submission_date 2003-08-25 _Accession_date 2003-08-26 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Vallurupalli Pramodh . . 2 Moore Peter B. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 253 "13C chemical shifts" 110 "15N chemical shifts" 20 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2003-10-16 original author . stop_ _Original_release_date 2003-10-16 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; The solution structure of the loop E region of the 5S rRNA from spinach chloroplasts ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 12527295 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Vallurupalli Pramodh . . 2 Moore Peter B. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 325 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 843 _Page_last 856 _Year 2003 _Details . loop_ _Keyword '5 S rRNA' L25 'loop E' NMR 'Spinacia oleracia' stop_ save_ ################################## # Molecular system description # ################################## save_system_Loop_E_of_5S_rRNA_from_spinach_chloroplasts _Saveframe_category molecular_system _Mol_system_name 'Loop E of 5S rRNA from spinach chloroplasts' _Abbreviation_common 'Loop E of 5S rRNA from spinach chloroplasts' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Loop E of 5S rRNA from spinach chloroplasts' $SPIN2 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'not present' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_SPIN2 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common 'Loop E of 5S rRNA from spinach chloroplasts' _Abbreviation_common SPIN2 _Molecular_mass 14468 _Mol_thiol_state 'not present' _Details ; The sequence consists of the Helix IV/V arm of spinanch chlorolast 5S rRNA. The ends on either side have been modified to make a construct suitable for NMR. Numbered sequentially in the following way to keep numbering consistent with the E. Coli sequence 68-88a,88b-108. ; ############################## # Polymer residue sequence # ############################## _Residue_count 42 _Mol_residue_sequence ; GGGUGACGAUACUGUAGGCG AGAGCCUGCGGAAAAAUAGC CC ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 68 G 2 69 G 3 70 G 4 71 U 5 72 G 6 73 A 7 74 C 8 75 G 9 76 A 10 77 U 11 78 A 12 79 C 13 80 U 14 81 G 15 82 U 16 83 A 17 84 G 18 85 G 19 86 C 20 87 G 21 88a A 22 88b G 23 89 A 24 90 G 25 91 C 26 92 C 27 93 U 28 94 G 29 95 C 30 96 G 31 97 G 32 98 A 33 99 A 34 100 A 35 101 A 36 102 A 37 103 U 38 104 A 39 105 G 40 106 C 41 107 C 42 108 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Organelle $SPIN2 Spinach 3562 Eukaryota Viridiplantae Spinacia oleracea Chloroplast stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $SPIN2 'enzymatic semisynthesis' . . . . . ; The RNA was invitro transcribed from a linearized template plasmid using T7 RNA polymerase. ; stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_Sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SPIN2 1.5 mM . NaCl 50 mM . Cacodylate 2.5 mM . EDTA 0.1 mM . D2O 10 % . H2O 90 % . stop_ save_ save_Sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SPIN2 1.5 mM . NaCl 50 mM . Cacodylate 2.5 mM . EDTA 0.1 mM . D2O 100 % . stop_ save_ save_Sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SPIN2 1.5 mM '[U->90% 13C; U->90% 15N]' NaCl 50 mM . Cacodylate 2.5 mM . EDTA 0.1 mM . D2O 10 % . H2O 90 % . stop_ save_ save_Sample_4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $SPIN2 1.5 mM '[U->90% 13C; U->90% 15N]' NaCl 50 mM . Cacodylate 2.5 mM . EDTA 0.1 mM . D2O 100 % . stop_ save_ ############################ # Computer software used # ############################ save_Felix _Saveframe_category software _Name FELIX _Version . _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version . _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 500 _Details . save_ save_NMR_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model UnityPlus _Field_strength 500 _Details . save_ save_NMR_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_1H-15NHSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name 1H-15NHSQC _Sample_label . save_ save_1H-13CHSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name 1H-13CHSQC _Sample_label . save_ save_1H-13CNOESY-HMQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name 1H-13CNOESY-HMQC _Sample_label . save_ save_HCcH_COSY_4 _Saveframe_category NMR_applied_experiment _Experiment_name 'HCcH COSY' _Sample_label . save_ save_Relay_HCcH_COSY_5 _Saveframe_category NMR_applied_experiment _Experiment_name 'Relay HCcH COSY' _Sample_label . save_ save_Double_Relay_HCcH_COSY_6 _Saveframe_category NMR_applied_experiment _Experiment_name 'Double Relay HCcH COSY' _Sample_label . save_ save_IPAP_HSQC_7 _Saveframe_category NMR_applied_experiment _Experiment_name 'IPAP HSQC' _Sample_label . save_ save_IPAP_HCcH_COSY_8 _Saveframe_category NMR_applied_experiment _Experiment_name 'IPAP HCcH COSY' _Sample_label . save_ save_IPAP_Relay_HCcH_COSY_9 _Saveframe_category NMR_applied_experiment _Experiment_name 'IPAP Relay HCcH COSY' _Sample_label . save_ save_NOESY_10 _Saveframe_category NMR_applied_experiment _Experiment_name NOESY _Sample_label . save_ save_DQF-COSY_11 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _Sample_label . save_ save_NMR_spec_expt__0_1 _Saveframe_category NMR_applied_experiment _Experiment_name 1H-15NHSQC _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_2 _Saveframe_category NMR_applied_experiment _Experiment_name 1H-13CHSQC _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_3 _Saveframe_category NMR_applied_experiment _Experiment_name 1H-13CNOESY-HMQC _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_4 _Saveframe_category NMR_applied_experiment _Experiment_name 'HCcH COSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_5 _Saveframe_category NMR_applied_experiment _Experiment_name 'Relay HCcH COSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_6 _Saveframe_category NMR_applied_experiment _Experiment_name 'Double Relay HCcH COSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_7 _Saveframe_category NMR_applied_experiment _Experiment_name 'IPAP HSQC' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_8 _Saveframe_category NMR_applied_experiment _Experiment_name 'IPAP HCcH COSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_9 _Saveframe_category NMR_applied_experiment _Experiment_name 'IPAP Relay HCcH COSY' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_10 _Saveframe_category NMR_applied_experiment _Experiment_name NOESY _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_11 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_Cond_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 . n/a temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.0 external indirect cylindrical external parallel 0.251449530 DSS H 1 'methyl protons' ppm 0.0 . . . . . 1.0 DSS N 15 'methyl protons' ppm 0.0 external indirect cylindrical external parallel 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_shifts _Saveframe_category assigned_chemical_shifts _Details ; Shifts for non exchangeble protons/carbons are at 25C while they are at 5C for exchangeble protons/nitrogens. ; loop_ _Sample_label $Sample_1 $Sample_2 $Sample_3 $Sample_4 stop_ _Sample_conditions_label $Cond_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'Loop E of 5S rRNA from spinach chloroplasts' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 G H8 H 8.11 0.02 . 2 . 1 G H1' H 5.79 0.02 . 3 . 1 G H2' H 4.88 0.02 . 4 . 1 G H3' H 4.70 0.02 . 5 . 1 G H4' H 4.52 0.02 . 6 . 1 G H5' H 4.24 0.02 . 7 . 1 G H5'' H 4.42 0.02 . 8 . 1 G H1 H 12.6 0.02 . 9 . 1 G C1' C 91.4 0.1 . 10 . 1 G C8 C 139.4 0.1 . 11 . 1 G N1 N 148.4 0.1 . 12 . 2 G H8 H 7.54 0.02 . 13 . 2 G H1' H 5.90 0.02 . 14 . 2 G H2' H 4.63 0.02 . 15 . 2 G H3' H 4.54 0.02 . 16 . 2 G H4' H 4.50 0.02 . 17 . 2 G H1 H 12.78 0.02 . 18 . 2 G C1' C 92.4 0.1 . 19 . 2 G C8 C 137.0 0.1 . 20 . 2 G N1 N 148.3 0.1 . 21 . 3 G H8 H 7.13 0.02 . 22 . 3 G H1' H 5.75 0.02 . 23 . 3 G H2' H 4.57 0.02 . 24 . 3 G H3' H 4.31 0.02 . 25 . 3 G H4' H 4.43 0.02 . 26 . 3 G H1 H 13.26 0.02 . 27 . 3 G C1' C 92.5 0.1 . 28 . 3 G C8 C 135.9 0.1 . 29 . 3 G N1 N 149.4 0.1 . 30 . 4 U H6 H 7.33 0.02 . 31 . 4 U H5 H 5.25 0.02 . 32 . 4 U H1' H 5.36 0.02 . 33 . 4 U H2' H 4.58 0.02 . 34 . 4 U H3' H 4.32 0.02 . 35 . 4 U H4' H 4.35 0.02 . 36 . 4 U H5' H 4.03 0.02 . 37 . 4 U H5'' H 4.43 0.02 . 38 . 4 U H3 H 11.33 0.02 . 39 . 4 U C1' C 93.7 0.1 . 40 . 4 U C6 C 139.4 0.1 . 41 . 4 U N3 N 158.3 0.1 . 42 . 4 U C5 C 104.7 0.1 . 43 . 5 G H8 H 7.95 0.02 . 44 . 5 G H1' H 5.53 0.02 . 45 . 5 G H2' H 4.37 0.02 . 46 . 5 G H3' H 4.87 0.02 . 47 . 5 G H4' H 4.45 0.02 . 48 . 5 G H1 H 10.56 0.02 . 49 . 5 G C1' C 89.9 0.1 . 50 . 5 G C8 C 137.7 0.1 . 51 . 5 G N1 N 147.8 0.1 . 52 . 6 A H8 H 7.86 0.02 . 53 . 6 A H2 H 7.95 0.02 . 54 . 6 A H1' H 5.92 0.02 . 55 . 6 A H2' H 4.84 0.02 . 56 . 6 A H3' H 4.10 0.02 . 57 . 6 A C1' C 93.4 0.1 . 58 . 6 A C8 C 139.8 0.1 . 59 . 6 A C2 C 154.1 0.1 . 60 . 7 C H6 H 7.01 0.02 . 61 . 7 C H5 H 4.89 0.02 . 62 . 7 C H1' H 5.16 0.02 . 63 . 7 C H2' H 4.43 0.02 . 64 . 7 C H3' H 4.24 0.02 . 65 . 7 C H4' H 4.28 0.02 . 66 . 7 C C1' C 93.1 0.1 . 67 . 7 C C6 C 139.7 0.1 . 68 . 7 C C5 C 96.9 0.1 . 69 . 8 G H8 H 7.54 0.02 . 70 . 8 G H1' H 5.45 0.02 . 71 . 8 G H2' H 4.65 0.02 . 72 . 8 G H3' H 4.53 0.02 . 73 . 8 G H4' H 4.43 0.02 . 74 . 8 G C1' C 92.1 0.1 . 75 . 8 G C8 C 137.7 0.1 . 76 . 9 A H8 H 7.66 0.02 . 77 . 9 A H2 H 7.90 0.02 . 78 . 9 A H1' H 5.72 0.02 . 79 . 9 A H2' H 4.53 0.02 . 80 . 9 A H3' H 4.50 0.02 . 81 . 9 A H4' H 4.42 0.02 . 82 . 9 A C1' C 92.4 0.1 . 83 . 9 A C8 C 139.1 0.1 . 84 . 9 A C2 C 155.2 0.1 . 85 . 10 U H6 H 7.12 0.02 . 86 . 10 U H5 H 4.92 0.02 . 87 . 10 U H1' H 5.11 0.02 . 88 . 10 U H2' H 4.26 0.02 . 89 . 10 U H3' H 4.44 0.02 . 90 . 10 U H4' H 4.35 0.02 . 91 . 10 U H3 H 12.00 0.02 . 92 . 10 U C1' C 92.9 0.1 . 93 . 10 U C6 C 134.8 0.1 . 94 . 10 U N3 N 160.6 0.1 . 95 . 10 U C5 C 104.1 0.1 . 96 . 11 A H8 H 7.66 0.02 . 97 . 11 A H2 H 7.24 0.02 . 98 . 11 A H1' H 5.67 0.02 . 99 . 11 A H2' H 4.61 0.02 . 100 . 11 A H3' H 4.40 0.02 . 101 . 11 A C1' C 92.5 0.1 . 102 . 11 A C8 C 139.7 0.1 . 103 . 11 A C2 C 153.6 0.1 . 104 . 12 C H6 H 7.44 0.02 . 105 . 12 C H5 H 5.61 0.02 . 106 . 12 C H3' H 4.07 0.02 . 107 . 12 C H4' H 4.14 0.02 . 108 . 12 C C6 C 140.5 0.1 . 109 . 12 C C5 C 99.26 0.1 . 110 . 13 U H6 H 7.59 0.02 . 111 . 13 U H5 H 5.61 0.02 . 112 . 13 U H1' H 5.50 0.02 . 113 . 13 U H2' H 4.20 0.02 . 114 . 13 U H3' H 4.44 0.02 . 115 . 13 U H4' H 4.31 0.02 . 116 . 13 U H3 H 11.50 0.02 . 117 . 13 U C1' C 93.5 0.1 . 118 . 13 U C6 C 141.3 0.1 . 119 . 13 U N3 N 159.0 0.1 . 120 . 13 U C5 C 105.3 0.1 . 121 . 14 G H8 H 7.78 0.02 . 122 . 14 G H1' H 5.66 0.02 . 123 . 14 G H2' H 4.62 0.02 . 124 . 14 G H3' H 4.40 0.02 . 125 . 14 G H1 H 12.77 0.02 . 126 . 14 G C1' C 92.4 0.1 . 127 . 14 G C8 C 137.3 0.1 . 128 . 14 G N1 N 148.2 0.1 . 129 . 15 U H6 H 7.60 0.02 . 130 . 15 U H5 H 5.42 0.02 . 131 . 15 U H1' H 5.38 0.02 . 132 . 15 U H2' H 4.24 0.02 . 133 . 15 U H3' H 4.54 0.02 . 134 . 15 U H4' H 4.39 0.02 . 135 . 15 U H3 H 11.58 0.02 . 136 . 15 U C1' C 93.3 0.1 . 137 . 15 U C6 C 140.8 0.1 . 138 . 15 U N3 N 159.3 0.1 . 139 . 15 U C5 C 104.6 0.1 . 140 . 16 A H8 H 8.29 0.02 . 141 . 16 A H2 H 6.93 0.02 . 142 . 16 A H1' H 5.94 0.02 . 143 . 16 A H2' H 4.72 0.02 . 144 . 16 A H3' H 4.71 0.02 . 145 . 16 A H4' H 4.43 0.02 . 146 . 16 A C1' C 91.9 0.1 . 147 . 16 A C8 C 140.8 0.1 . 148 . 16 A C2 C 153.0 0.1 . 149 . 17 G H8 H 7.02 0.02 . 150 . 17 G H1' H 5.47 0.02 . 151 . 17 G H2' H 4.43 0.02 . 152 . 17 G H3' H 4.35 0.02 . 153 . 17 G H4' H 4.44 0.02 . 154 . 17 G H1 H 12.72 0.02 . 155 . 17 G C1' C 92.3 0.1 . 156 . 17 G C8 C 136.0 0.1 . 157 . 17 G N1 N 148.6 0.1 . 158 . 18 G H8 H 7.26 0.02 . 159 . 18 G H1' H 5.73 0.02 . 160 . 18 G H2' H 4.38 0.02 . 161 . 18 G H3' H 4.50 0.02 . 162 . 18 G H4' H 4.40 0.02 . 163 . 18 G H1 H 13.21 0.02 . 164 . 18 G C1' C 92.4 0.1 . 165 . 18 G C8 C 136.3 0.1 . 166 . 18 G N1 N 149.3 0.1 . 167 . 19 C H6 H 7.37 0.02 . 168 . 19 C H5 H 5.08 0.02 . 169 . 19 C H1' H 5.55 0.02 . 170 . 19 C H2' H 4.50 0.02 . 171 . 19 C H3' H 4.41 0.02 . 172 . 19 C H4' H 4.36 0.02 . 173 . 19 C C1' C 93.2 0.1 . 174 . 19 C C6 C 139.9 0.1 . 175 . 19 C C5 C 98.1 0.1 . 176 . 20 G H8 H 7.40 0.02 . 177 . 20 G H1' H 5.74 0.02 . 178 . 20 G H2' H 4.31 0.02 . 179 . 20 G H3' H 4.59 0.02 . 180 . 20 G H4' H 4.34 0.02 . 181 . 20 G H1 H 10.41 0.02 . 182 . 20 G C1' C 93.3 0.1 . 183 . 20 G C8 C 136.6 0.1 . 184 . 20 G N1 N 146.5 0.1 . 185 . 21 A H8 H 8.21 0.02 . 186 . 21 A H2 H 7.87 0.02 . 187 . 21 A H1' H 5.67 0.02 . 188 . 21 A H2' H 4.66 0.02 . 189 . 21 A H3' H 4.38 0.02 . 190 . 21 A H4' H 4.16 0.02 . 191 . 21 A C1' C 92.0 0.1 . 192 . 21 A C8 C 142.2 0.1 . 193 . 21 A C2 C 156.0 0.1 . 194 . 22 G H8 H 7.55 0.02 . 195 . 22 G H1' H 5.10 0.02 . 196 . 22 G H2' H 4.15 0.02 . 197 . 22 G H3' H 4.51 0.02 . 198 . 22 G H4' H 4.20 0.02 . 199 . 22 G H1 H 10.52 0.02 . 200 . 22 G C1' C 91.7 0.1 . 201 . 22 G C8 C 137.8 0.1 . 202 . 22 G N1 N 147.7 0.1 . 203 . 22 G C2 C 154.8 0.1 . 204 . 23 A H8 H 8.13 0.02 . 205 . 23 A H2 H 8.19 0.02 . 206 . 23 A H1' H 6.05 0.02 . 207 . 23 A H2' H 4.57 0.02 . 208 . 23 A H3' H 4.95 0.02 . 209 . 23 A H4' H 4.40 0.02 . 210 . 23 A C1' C 91.4 0.1 . 211 . 23 A C8 C 140.8 0.1 . 212 . 24 G H8 H 7.92 0.02 . 213 . 24 G H1' H 3.85 0.02 . 214 . 24 G H2' H 4.27 0.02 . 215 . 24 G H3' H 4.11 0.02 . 216 . 24 G H4' H 4.19 0.02 . 217 . 24 G H1 H 12.96 0.02 . 218 . 24 G C1' C 92.6 0.1 . 219 . 24 G C8 C 137.7 0.1 . 220 . 24 G N1 N 149.0 0.1 . 221 . 25 C H6 H 7.63 0.02 . 222 . 25 C H5 H 5.17 0.02 . 223 . 25 C H1' H 5.51 0.02 . 224 . 25 C H2' H 4.42 0.02 . 225 . 25 C H3' H 4.39 0.02 . 226 . 25 C C1' C 93.4 0.1 . 227 . 25 C C6 C 141.6 0.1 . 228 . 25 C C5 C 97.2 0.1 . 229 . 26 C H6 H 7.73 0.02 . 230 . 26 C H5 H 5.48 0.02 . 231 . 26 C H1' H 5.48 0.02 . 232 . 26 C H2' H 4.37 0.02 . 233 . 26 C H3' H 4.45 0.02 . 234 . 26 C C1' C 93.7 0.1 . 235 . 26 C C6 C 141.7 0.1 . 236 . 26 C C5 C 98.1 0.1 . 237 . 27 U H6 H 7.81 0.02 . 238 . 27 U H5 H 5.41 0.02 . 239 . 27 U H1' H 5.55 0.02 . 240 . 27 U H2' H 4.52 0.02 . 241 . 27 U H3' H 4.54 0.02 . 242 . 27 U H4' H 4.41 0.02 . 243 . 27 U H3 H 13.50 0.02 . 244 . 27 U C1' C 92.8 0.1 . 245 . 27 U C6 C 142.2 0.1 . 246 . 27 U N3 N 163.3 0.1 . 247 . 27 U C5 C 104.0 0.1 . 248 . 28 G H8 H 7.69 0.02 . 249 . 28 G H1' H 5.74 0.02 . 250 . 28 G H2' H 4.51 0.02 . 251 . 28 G H3' H 4.36 0.02 . 252 . 28 G H4' H 4.45 0.02 . 253 . 28 G H1 H 10.77 0.02 . 254 . 28 G C1' C 92.7 0.1 . 255 . 28 G C8 C 137.4 0.1 . 256 . 28 G N1 N 144.7 0.1 . 257 . 29 C H6 H 7.50 0.02 . 258 . 29 C H5 H 5.24 0.02 . 259 . 29 C H1' H 5.47 0.02 . 260 . 29 C H2' H 4.42 0.02 . 261 . 29 C H3' H 4.43 0.02 . 262 . 29 C H4' H 4.31 0.02 . 263 . 29 C C1' C 92.7 0.1 . 264 . 29 C C6 C 140.6 0.1 . 265 . 29 C C5 C 97.8 0.1 . 266 . 30 G H8 H 7.34 0.02 . 267 . 30 G H1' H 5.67 0.02 . 268 . 30 G H2' H 4.71 0.02 . 269 . 30 G H3' H 4.19 0.02 . 270 . 30 G H4' H 4.45 0.02 . 271 . 30 G H1 H 10.17 0.02 . 272 . 30 G C1' C 93.4 0.1 . 273 . 30 G C8 C 137.6 0.1 . 274 . 30 G N1 N 143.2 0.1 . 275 . 31 G H8 H 6.95 0.02 . 276 . 31 G H1' H 5.63 0.02 . 277 . 31 G H2' H 4.69 0.02 . 278 . 31 G H3' H 4.44 0.02 . 279 . 31 G H4' H 4.41 0.02 . 280 . 31 G H1 H 12.83 0.02 . 281 . 31 G C1' C 92.7 0.1 . 282 . 31 G C8 C 136.1 0.1 . 283 . 31 G N1 N 148.6 0.1 . 284 . 32 A H8 H 7.81 0.02 . 285 . 32 A H2 H 8.0 0.02 . 286 . 32 A H1' H 5.77 0.02 . 287 . 32 A H2' H 4.38 0.02 . 288 . 32 A H3' H 4.69 0.02 . 289 . 32 A H4' H 4.45 0.02 . 290 . 32 A C1' C 93.6 0.1 . 291 . 32 A C8 C 139.7 0.1 . 292 . 32 A C2 C 155.5 0.1 . 293 . 33 A H8 H 7.76 0.02 . 294 . 33 A H2 H 7.69 0.02 . 295 . 33 A H1' H 5.80 0.02 . 296 . 33 A H2' H 4.88 0.02 . 297 . 33 A H3' H 4.32 0.02 . 298 . 33 A H4' H 4.64 0.02 . 299 . 33 A C1' C 93.5 0.1 . 300 . 33 A C8 C 140.4 0.1 . 301 . 33 A C2 C 154.2 0.1 . 302 . 34 A H8 H 7.63 0.02 . 303 . 34 A H2 H 7.97 0.02 . 304 . 34 A H1' H 5.45 0.02 . 305 . 34 A H2' H 4.65 0.02 . 306 . 34 A H3' H 4.53 0.02 . 307 . 34 A H4' H 4.41 0.02 . 308 . 34 A C1' C 91.8 0.1 . 309 . 34 A C8 C 138.8 0.1 . 310 . 34 A C2 C 154.8 0.1 . 311 . 35 A H8 H 7.42 0.02 . 312 . 35 A H2 H 7.89 0.02 . 313 . 35 A H1' H 5.16 0.02 . 314 . 35 A H2' H 4.55 0.02 . 315 . 35 A H3' H 4.32 0.02 . 316 . 35 A H4' H 4.37 0.02 . 317 . 35 A C1' C 92.0 0.1 . 318 . 35 A C8 C 139.1 0.1 . 319 . 35 A C2 C 154.3 0.1 . 320 . 36 A H8 H 7.35 0.02 . 321 . 36 A H2 H 7.61 0.02 . 322 . 36 A H1' H 5.25 0.02 . 323 . 36 A H2' H 4.40 0.02 . 324 . 36 A H3' H 4.27 0.02 . 325 . 36 A H4' H 4.34 0.02 . 326 . 36 A C1' C 92.4 0.1 . 327 . 36 A C8 C 139.7 0.1 . 328 . 36 A C2 C 154.4 0.1 . 329 . 37 U H6 H 7.25 0.02 . 330 . 37 U H5 H 4.82 0.02 . 331 . 37 U H1' H 5.31 0.02 . 332 . 37 U H2' H 4.22 0.02 . 333 . 37 U H3' H 4.42 0.02 . 334 . 37 U H4' H 4.37 0.02 . 335 . 37 U H3 H 12.11 0.02 . 336 . 37 U C1' C 92.1 0.1 . 337 . 37 U C6 C 140.4 0.1 . 338 . 37 U N3 N 160.6 0.1 . 339 . 37 U C5 C 104.1 0.1 . 340 . 38 A H8 H 7.86 0.02 . 341 . 38 A H2 H 7.16 0.02 . 342 . 38 A H1' H 5.83 0.02 . 343 . 38 A H2' H 4.83 0.02 . 344 . 38 A H3' H 4.43 0.02 . 345 . 38 A H4' H 4.48 0.02 . 346 . 38 A C1' C 92.6 0.1 . 347 . 38 A C8 C 139.7 0.1 . 348 . 38 A C2 C 153.6 0.1 . 349 . 39 G H8 H 7.25 0.02 . 350 . 39 G H1' H 4.32 0.02 . 351 . 39 G H2' H 4.23 0.02 . 352 . 39 G H3' H 4.23 0.02 . 353 . 39 G H4' H 4.28 0.02 . 354 . 39 G H1 H 11.50 0.02 . 355 . 39 G C1' C 92.1 0.1 . 356 . 39 G C8 C 135.9 0.1 . 357 . 39 G N1 N 145.7 0.1 . 358 . 40 C H6 H 7.61 0.02 . 359 . 40 C H5 H 5.28 0.02 . 360 . 40 C H1' H 5.41 0.02 . 361 . 40 C H2' H 4.19 0.02 . 362 . 40 C H3' H 4.43 0.02 . 363 . 40 C H4' H 4.32 0.02 . 364 . 40 C C1' C 93.5 0.1 . 365 . 40 C C6 C 140.9 0.1 . 366 . 40 C C5 C 97.5 0.1 . 367 . 41 C H6 H 7.78 0.02 . 368 . 41 C H5 H 5.39 0.02 . 369 . 41 C H1' H 5.47 0.02 . 370 . 41 C H2' H 4.24 0.02 . 371 . 41 C H3' H 4.45 0.02 . 372 . 41 C H4' H 4.33 0.02 . 373 . 41 C C1' C 93.9 0.1 . 374 . 41 C C6 C 141.8 0.1 . 375 . 41 C C5 C 97.7 0.1 . 376 . 42 C H6 H 7.65 0.02 . 377 . 42 C H5 H 5.47 0.02 . 378 . 42 C H1' H 5.73 0.02 . 379 . 42 C H2' H 3.98 0.02 . 380 . 42 C H3' H 4.15 0.02 . 381 . 42 C C1' C 92.4 0.1 . 382 . 42 C C6 C 142.1 0.1 . 383 . 42 C C5 C 98.4 0.1 . stop_ save_