data_5811 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H Chemical Shift Assignments for quasi-repetitive arginine/glycine/ tyrosine-rich domains within glycine-rich RNA binding proteins ; _BMRB_accession_number 5811 _BMRB_flat_file_name bmr5811.str _Entry_type original _Submission_date 2003-05-27 _Accession_date 2003-05-27 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kumaki Yasuhiro . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 coupling_constants 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 47 "coupling constants" 4 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2005-05-26 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 5812 'Chemical shift of GGYGGGRRDG' stop_ _Original_release_date 2005-05-26 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Side chain-side chain interactions of arginine with tyrosine and aspartic acid in Arg/Gly/Tyr-rich domains within plant glycine-rich RNA binding proteins ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 15269237 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kumaki Yasuhiro . . 2 Nitta K. . . 3 Hikichi K. . . 4 Matsumoto T. . . 5 Matsushima N. . . stop_ _Journal_abbreviation 'J. Biochem.' _Journal_volume 136 _Journal_issue 1 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 29 _Page_last 37 _Year 2004 _Details ; quasi-repetitive arginine/glycine/ tyrosine-rich domains within glycine-rich RNA binding protein ; loop_ _Keyword 'arginine/glycine/ tyrosine-rich domains' 'glycine-rich RNA binding protein' quasi-repetitive stop_ save_ ################################## # Molecular system description # ################################## save_GGRRDGGYGG _Saveframe_category molecular_system _Mol_system_name 'GGRRDGGYGG monomer' _Abbreviation_common GGRRDGGYGG _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'GGRRDGGYGG subunit 1' $glycine-rich_RNA-binding_proteins stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'not present' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_glycine-rich_RNA-binding_proteins _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'glycine-rich RNA-binding proteins' _Abbreviation_common 'glycine-rich RNA-binding proteins' _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 10 _Mol_residue_sequence GGRRDGGYGG loop_ _Residue_seq_code _Residue_label 1 GLY 2 GLY 3 ARG 4 ARG 5 ASP 6 GLY 7 GLY 8 TYR 9 GLY 10 GLY stop_ _Sequence_homology_query_date 2005-11-24 _Sequence_homology_query_revised_last_date 2005-06-17 save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $glycine-rich_RNA-binding_proteins 'Zea mays' 4577 Eukaryota Viridiplantae Zea mays stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $glycine-rich_RNA-binding_proteins 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $glycine-rich_RNA-binding_proteins 12 mM . stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer _Saveframe_category NMR_spectrometer _Manufacturer JEOL _Model ALPHA _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_TOCSY_1 _Saveframe_category NMR_applied_experiment _Experiment_name TOCSY _Sample_label . save_ save_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name NOESY _Sample_label . save_ save_ROESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name ROESY _Sample_label . save_ save_DQF-COSY_4 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _Sample_label . save_ save_NMR_spec_expt__0_1 _Saveframe_category NMR_applied_experiment _Experiment_name TOCSY _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_2 _Saveframe_category NMR_applied_experiment _Experiment_name NOESY _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_3 _Saveframe_category NMR_applied_experiment _Experiment_name ROESY _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_4 _Saveframe_category NMR_applied_experiment _Experiment_name DQF-COSY _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_sample_cond_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 4.3 0.2 pH temperature 278 1 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.0 internal direct . . . 1.0 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_shift_set_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_cond_1 _Chem_shift_reference_set_label $chemical_shift_reference _Mol_system_component_name 'GGRRDGGYGG subunit 1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 GLY HA2 H 3.89 0.02 1 2 . 1 GLY HA3 H 3.89 0.02 1 3 . 2 GLY H H 8.67 0.02 1 4 . 2 GLY HA2 H 4.03 0.02 1 5 . 2 GLY HA3 H 4.03 0.02 1 6 . 3 ARG H H 8.55 0.02 1 7 . 3 ARG HA H 4.31 0.02 1 8 . 3 ARG HB2 H 1.75 0.02 2 9 . 3 ARG HB3 H 1.83 0.02 2 10 . 3 ARG HG2 H 1.62 0.02 1 11 . 3 ARG HG3 H 1.62 0.02 1 12 . 3 ARG HD2 H 3.17 0.02 1 13 . 3 ARG HD3 H 3.17 0.02 1 14 . 3 ARG HE H 7.41 0.02 1 15 . 4 ARG H H 8.75 0.02 1 16 . 4 ARG HA H 4.36 0.02 1 17 . 4 ARG HB2 H 1.77 0.02 2 18 . 4 ARG HB3 H 1.86 0.02 2 19 . 4 ARG HG2 H 1.61 0.02 1 20 . 4 ARG HG3 H 1.61 0.02 1 21 . 4 ARG HD2 H 3.17 0.02 1 22 . 4 ARG HD3 H 3.17 0.02 1 23 . 4 ARG HE H 7.22 0.02 1 24 . 5 ASP H H 8.52 0.02 1 25 . 5 ASP HA H 4.63 0.02 1 26 . 5 ASP HB2 H 2.75 0.02 1 27 . 5 ASP HB3 H 2.75 0.02 1 28 . 6 GLY H H 8.51 0.02 1 29 . 6 GLY HA2 H 3.93 0.02 2 30 . 6 GLY HA3 H 3.98 0.02 2 31 . 7 GLY H H 8.35 0.02 1 32 . 7 GLY HA2 H 3.9 0.02 1 33 . 7 GLY HA3 H 3.9 0.02 1 34 . 8 TYR H H 8.29 0.02 1 35 . 8 TYR HA H 4.55 0.02 1 36 . 8 TYR HB2 H 2.98 0.02 2 37 . 8 TYR HB3 H 3.06 0.02 2 38 . 8 TYR HD1 H 7.15 0.02 1 39 . 8 TYR HD2 H 7.15 0.02 1 40 . 8 TYR HE1 H 6.84 0.02 1 41 . 8 TYR HE2 H 6.84 0.02 1 42 . 9 GLY H H 8.56 0.02 1 43 . 9 GLY HA2 H 3.9 0.02 1 44 . 9 GLY HA3 H 3.9 0.02 1 45 . 10 GLY H H 7.69 0.02 1 46 . 10 GLY HA2 H 3.76 0.02 1 47 . 10 GLY HA3 H 3.76 0.02 1 stop_ save_ ######################## # Coupling constants # ######################## save_J_values_set_1 _Saveframe_category coupling_constants _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_cond_1 _Spectrometer_frequency_1H 500 _Mol_system_component_name 'GGRRDGGYGG subunit 1' _Text_data_format . _Text_data . loop_ _Coupling_constant_ID _Coupling_constant_code _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_name _Coupling_constant_value _Coupling_constant_min_value _Coupling_constant_max_value _Coupling_constant_value_error 1 3JHNHA 3 ARG H 3 ARG HA 6.4 . . 0.8 2 3JHNHA 4 ARG H 4 ARG HA 6.4 . . 0.8 3 3JHNHA 5 ASP H 5 ASP HA 7.3 . . 0.8 4 3JHNHA 8 TYR H 8 TYR HA 7.3 . . 0.8 stop_ save_