data_4266 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; The Solution Structure of a Superpotent B-chain-shortened Single-replacement Insulin Analogue ; _BMRB_accession_number 4266 _BMRB_flat_file_name bmr4266.str _Entry_type original _Submission_date 1998-11-08 _Accession_date 1998-11-08 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kurapkat Guenther . . 2 Siedentop Michael . . 3 Gattner Hans-Georg . . 4 Hagelstein Michael . . 5 Brandenburg Dietrich . . 6 Grotzinger Joachim . . 7 Wollmer Axel . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 241 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2002-08-12 update BMRB 'Modify the saveframe name.' 1999-10-05 original author 'Original release.' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Kurapkat, G., Siedentop, M., Gattner, H-G., Hagelstein, M., Brandenburg, D., Grotzinger, J., and Wollmer, A., "The Solution Structure of a Superpotent B-chain-shortened Single-replacement Insulin Analogue," Protein Sci. 8, 499- 508 (1999). ; _Citation_title ; The Solution Structure of a Superpotent B-chain-shortend Single-replacement Insulin Analogue ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code 99190073 _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kurapkat Guenther . . 2 Siedentop Michael . . 3 Gattner Hans-Georg . . 4 Hagelstein Michael . . 5 Brandenburg Dietrich . . 6 Grotzinger Joachim . . 7 Wollmer Axel . . stop_ _Journal_abbreviation 'Protein Sci.' _Journal_name_full 'Protein Science' _Journal_volume 8 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 499 _Page_last 508 _Year 1999 _Details . loop_ _Keyword insulin structure 'active conformation' 'NMR spectroscopy' 'CD spectroscopy' receptor_binding superpotency stop_ save_ ################################## # Molecular system description # ################################## save_system_D-AlaB26_DTI-amide _Saveframe_category molecular_system _Mol_system_name [D-AlaB26]destetra(B27-B30)insulin-B26-amide _Abbreviation_common [D-AlaB26]DTI-amide _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label [D-AlaB26]DTI-amide_A-chain $D-AlaB26_DTI-amide [D-AlaB26]DTI-amide_B-chain $D-AlaB26_DTI-amide stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state . loop_ _Magnetic_equivalence_ID _Magnetically_equivalent_system_component 1 [D-AlaB26]DTI-amide_A-chain 2 [D-AlaB26]DTI-amide_B-chain stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_D-AlaB26_DTI-amide _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common [D-AlaB26]destetra(B27-B30)insulin-B26-amide _Abbreviation_common [D-AlaB26]DTI-amide _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 47 _Mol_residue_sequence ; GIVEQCCTSICSLYQLENYC NFVNQHLCGSHLVEALYLVC GERGFFA ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 ILE 3 VAL 4 GLU 5 GLN 6 CYS 7 CYS 8 THR 9 SER 10 ILE 11 CYS 12 SER 13 LEU 14 TYR 15 GLN 16 LEU 17 GLU 18 ASN 19 TYR 20 CYS 21 ASN 22 PHE 23 VAL 24 ASN 25 GLN 26 HIS 27 LEU 28 CYS 29 GLY 30 SER 31 HIS 32 LEU 33 VAL 34 GLU 35 ALA 36 LEU 37 TYR 38 LEU 39 VAL 40 CYS 41 GLY 42 GLU 43 ARG 44 GLY 45 PHE 46 PHE 47 ALA stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-10-20 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 1015 "insulin B chain" 53.19 30 100.00 100.00 4.26e-08 BMRB 1021 "insulin B chain" 51.06 30 100.00 100.00 2.32e-07 BMRB 1025 "insulin B chain" 53.19 30 100.00 100.00 4.26e-08 BMRB 1444 "insulin B chain" 53.19 30 100.00 100.00 4.63e-08 BMRB 1633 "insulin B chain" 53.19 30 100.00 100.00 4.26e-08 PDB 1AI0 "R6 Human Insulin Hexamer (Non-Symmetric), Nmr, 10 Structures" 53.19 30 100.00 100.00 4.26e-08 PDB 1AIY "R6 Human Insulin Hexamer (Symmetric), Nmr, 10 Structures" 53.19 30 100.00 100.00 4.26e-08 PDB 1APH "Conformational Changes In Cubic Insulin Crystals In The Ph Range 7-11" 53.19 30 100.00 100.00 4.63e-08 PDB 1B17 "Ph Affects Glu B13 Switching And Sulfate Binding In Cubic Insulin Crystals (Ph 5.00 Coordinates)" 53.19 30 100.00 100.00 4.63e-08 PDB 1B18 "Ph Affects Glu B13 Switching And Sulfate Binding In Cubic Insulin Crystals (Ph 5.53 Coordinates)" 53.19 30 100.00 100.00 4.63e-08 PDB 1B19 "Ph Affects Glu B13 Switching And Sulfate Binding In Cubic Insulin Crystals (Ph 5.80 Coordinates)" 53.19 30 100.00 100.00 4.63e-08 PDB 1B2A "Ph Affects Glu B13 Switching And Sulfate Binding In Cubic Insulin Crystals (Ph 6.00 Coordinates)" 53.19 30 100.00 100.00 4.63e-08 PDB 1B2B "Ph Affects Glu B13 Switching And Sulfate Binding In Cubic Insulin Crystals (Ph 6.16 Coordinates)" 53.19 30 100.00 100.00 4.63e-08 PDB 1B2C "Ph Affects Glu B13 Switching And Sulfate Binding In Cubic Insulin Crystals (Ph 6.26 Coordinates)" 53.19 30 100.00 100.00 4.63e-08 PDB 1B2D "Ph Affects Glu B13 Switching And Sulfate Binding In Cubic Insulin Crystals (Ph 6.35 Coordinates)" 53.19 30 100.00 100.00 4.63e-08 PDB 1B2E "Ph Affects Glu B13 Switching And Sulfate Binding In Cubic Insulin Crystals (Ph 6.50 Coordinates)" 53.19 30 100.00 100.00 4.63e-08 PDB 1B2F "Ph Affects Glu B13 Switching And Sulfate Binding In Cubic Insulin Crystals (Ph 6.98 Coordinates)" 53.19 30 100.00 100.00 4.63e-08 PDB 1B2G "Ph Affects Glu B13 Switching And Sulfate Binding In Cubic Insulin Crystals (Ph 9.00 Coordinates)" 53.19 30 100.00 100.00 4.63e-08 PDB 1BEN "Insulin Complexed With 4-Hydroxybenzamide" 51.06 30 100.00 100.00 2.61e-07 PDB 1BPH "Conformational Changes In Cubic Insulin Crystals In The Ph Range 7-11" 53.19 30 100.00 100.00 4.63e-08 PDB 1BZV "[d-Alab26]-Des(B27-B30)-Insulin-B26-Amide A Superpotent Single-Replacement Insulin Analogue, Nmr, Minimized Average Structure" 53.19 26 100.00 100.00 8.95e-08 PDB 1CPH "Conformational Changes In Cubic Insulin Crystals In The Ph Range 7-11" 53.19 30 100.00 100.00 4.63e-08 PDB 1DPH "Conformational Changes In Cubic Insulin Crystals In The Ph Range 7-11" 53.19 30 100.00 100.00 4.63e-08 PDB 1EV3 "Structure Of The Rhombohedral Form Of The M-cresol/insulin R6 Hexamer" 53.19 30 100.00 100.00 4.26e-08 PDB 1EV6 "Structure Of The Monoclinic Form Of The M-CresolINSULIN R6 Hexamer" 53.19 30 100.00 100.00 4.26e-08 PDB 1EVR "The Structure Of The ResorcinolINSULIN R6 HEXAMER" 53.19 30 100.00 100.00 4.26e-08 PDB 1FU2 "First Protein Structure Determined From X-Ray Powder Diffraction Data" 53.19 30 100.00 100.00 4.26e-08 PDB 1FUB "First Protein Structure Determined From X-Ray Powder Diffraction Data" 53.19 30 100.00 100.00 4.26e-08 PDB 1G7A "1.2 A Structure Of T3r3 Human Insulin At 100 K" 53.19 30 100.00 100.00 4.26e-08 PDB 1G7B "1.3 A Structure Of T3r3 Human Insulin At 100 K" 53.19 30 100.00 100.00 4.26e-08 PDB 1GUJ "Insulin At Ph 2: Structural Analysis Of The Conditions Promoting Insulin Fibre Formation." 53.19 30 100.00 100.00 4.26e-08 PDB 1HIS "Structure And Dynamics Of Des-Pentapeptide-Insulin In Solution: The Molten-Globule Hypothesis" 53.19 25 100.00 100.00 8.93e-08 PDB 1HTV "Crystal Structure Of Destripeptide (B28-B30) Insulin" 53.19 27 100.00 100.00 7.58e-08 PDB 1J73 "Crystal Structure Of An Unstable Insulin Analog With Native Activity." 53.19 30 100.00 100.00 4.26e-08 PDB 1JCA "Non-standard Design Of Unstable Insulin Analogues With Enhanced Activity" 53.19 30 100.00 100.00 4.26e-08 PDB 1JCO "Solution Structure Of The Monomeric [thr(B27)->pro,Pro(B28)- >thr] Insulin Mutant (Pt Insulin)" 53.19 30 100.00 100.00 4.26e-08 PDB 1LPH "Lys(B28)pro(B29)-Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 1M5A "Crystal Structure Of 2-Co(2+)-Insulin At 1.2a Resolution" 53.19 30 100.00 100.00 4.63e-08 PDB 1MPJ "X-Ray Crystallographic Studies On Hexameric Insulins In The Presence Of Helix-Stabilizing Agents, Thiocyanate, Methylparaben An" 53.19 30 100.00 100.00 4.63e-08 PDB 1MSO "T6 Human Insulin At 1.0 A Resolution" 53.19 30 100.00 100.00 4.26e-08 PDB 1OS3 "Dehydrated T6 Human Insulin At 100 K" 53.19 30 100.00 100.00 4.26e-08 PDB 1OS4 "Dehydrated T6 Human Insulin At 295 K" 53.19 30 100.00 100.00 4.26e-08 PDB 1PID "Bovine Despentapeptide Insulin" 53.19 25 100.00 100.00 8.93e-08 PDB 1Q4V "Crystal Structure Of Allo-Ilea2-Insulin, An Inactive Chiral Analogue: Implications For The Mechanism Of Receptor" 53.19 30 100.00 100.00 4.26e-08 PDB 1RWE "Enhancing The Activity Of Insulin At Receptor Edge: Crystal Structure And Photo-Cross-Linking Of A8 Analogues" 53.19 30 100.00 100.00 4.26e-08 PDB 1SF1 "Nmr Structure Of Human Insulin Under Amyloidogenic Condition, 15 Structures" 53.19 30 100.00 100.00 4.26e-08 PDB 1TRZ "Crystallographic Evidence For Dual Coordination Around Zinc In The T3r3 Human Insulin Hexamer" 53.19 30 100.00 100.00 4.26e-08 PDB 1TYL "The Structure Of A Complex Of Hexameric Insulin And 4'- Hydroxyacetanilide" 53.19 30 100.00 100.00 4.26e-08 PDB 1TYM "The Structure Of A Complex Of Hexameric Insulin And 4'- Hydroxyacetanilide" 53.19 30 100.00 100.00 4.26e-08 PDB 1UZ9 "Crystallographic And Solution Studies Of N-Lithocholyl Insulin: A New Generation Of Prolonged-Acting Insulins." 53.19 29 100.00 100.00 5.28e-08 PDB 1WAV "Crystal Structure Of Form B Monoclinic Crystal Of Insulin" 53.19 30 100.00 100.00 4.63e-08 PDB 1XDA "Structure Of Insulin" 53.19 29 100.00 100.00 5.28e-08 PDB 1XGL "Human Insulin Disulfide Isomer, Nmr, 10 Structures" 53.19 30 100.00 100.00 4.26e-08 PDB 1XW7 "Diabetes-Associated Mutations In Human Insulin: Crystal Structure And Photo-Cross-Linking Studies Of A-Chain Variant Insulin Wa" 53.19 30 100.00 100.00 4.26e-08 PDB 1ZEG "Structure Of B28 Asp Insulin In Complex With Phenol" 53.19 30 100.00 100.00 4.35e-08 PDB 1ZEH "Structure Of Insulin" 53.19 30 100.00 100.00 4.35e-08 PDB 1ZNI Insulin 53.19 30 100.00 100.00 4.63e-08 PDB 1ZNJ "Insulin, Monoclinic Crystal Form" 53.19 30 100.00 100.00 4.26e-08 PDB 2A3G "The Structure Of T6 Bovine Insulin" 53.19 30 100.00 100.00 4.63e-08 PDB 2AIY "R6 Human Insulin Hexamer (Symmetric), Nmr, 20 Structures" 53.19 30 100.00 100.00 4.26e-08 PDB 2BN1 "Insulin After A High Dose X-ray Burn" 53.19 30 100.00 100.00 4.63e-08 PDB 2BN3 "Insulin Before A High Dose X-Ray Burn" 53.19 30 100.00 100.00 4.63e-08 PDB 2C8Q "Insuline(1sec) And Uv Laser Excited Fluorescence" 53.19 29 100.00 100.00 5.28e-08 PDB 2C8R "Insuline(60sec) And Uv Laser Excited Fluorescence" 53.19 29 100.00 100.00 5.28e-08 PDB 2CEU "Despentapeptide Insulin In Acetic Acid (Ph 2)" 53.19 25 100.00 100.00 8.93e-08 PDB 2EFA "Neutron Crystal Structure Of Cubic Insulin At Pd6.6" 53.19 30 100.00 100.00 4.63e-08 PDB 2G4M "Insulin Collected At 2.0 A Wavelength" 53.19 30 100.00 100.00 4.63e-08 PDB 2G54 "Crystal Structure Of Zn-Bound Human Insulin-Degrading Enzyme In Complex With Insulin B Chain" 53.19 30 100.00 100.00 4.26e-08 PDB 2G56 "Crystal Structure Of Human Insulin-degrading Enzyme In Complex With Insulin B Chain" 53.19 30 100.00 100.00 4.26e-08 PDB 2HIU "Nmr Structure Of Human Insulin In 20% Acetic Acid, Zinc- Free, 10 Structures" 53.19 30 100.00 100.00 4.26e-08 PDB 2INS "The Structure Of Des-Phe B1 Bovine Insulin" 51.06 29 100.00 100.00 2.70e-07 PDB 2JV1 "Nmr Structure Of Human Insulin Monomer In 35% Cd3cn Zinc Free, 50 Structures" 53.19 30 100.00 100.00 4.26e-08 PDB 2KJJ "Dynamics Of Insulin Probed By 1h-Nmr Amide Proton Exchange A Flexibility Of The Receptor-Binding Surface" 53.19 30 100.00 100.00 4.26e-08 PDB 2KXK "Human Insulin Mutant A22gly-B31lys-B32arg" 53.19 32 100.00 100.00 3.35e-08 PDB 2LGB "Modified A22gly-B31arg Human Insulin" 53.19 31 100.00 100.00 4.07e-08 PDB 2M1D "Biosynthetic Engineered B28k-b29p Human Insulin Monomer Structure In In Water/acetonitrile Solutions." 53.19 30 100.00 100.00 4.26e-08 PDB 2M1E "Biosynthetic Engineered B28k-b29p Human Insulin Monomer Structure In In Water Solutions" 53.19 30 100.00 100.00 4.26e-08 PDB 2MVC "Solution Structure Of Human Insulin At Ph 1.9" 53.19 30 100.00 100.00 4.26e-08 PDB 2OLY "Structure Of Human Insulin In Presence Of Urea At Ph 7.0" 53.19 30 100.00 100.00 4.26e-08 PDB 2OLZ "Structure Of Human Insulin In Presence Of Thiocyanate At Ph 7.0" 53.19 30 100.00 100.00 4.26e-08 PDB 2OM0 "Structure Of Human Insulin In Presence Of Urea At Ph 6.5" 53.19 30 100.00 100.00 4.26e-08 PDB 2OM1 "Structure Of Human Insulin In Presence Of Thiocyanate At Ph 6.5" 53.19 30 100.00 100.00 4.26e-08 PDB 2OMG "Structure Of Human Insulin Cocrystallized With Protamine And Urea" 53.19 30 100.00 100.00 4.26e-08 PDB 2OMH "Structure Of Human Insulin Cocrystallized With Arg-12 Peptide In Presence Of Urea" 53.19 30 100.00 100.00 4.26e-08 PDB 2OMI "Structure Of Human Insulin Cocrystallized With Protamine" 53.19 30 100.00 100.00 4.26e-08 PDB 2QIU "Structure Of Human Arg-Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 2R34 "Crystal Structure Of Mn Human Arg-Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 2R35 "Crystal Structure Of Rb Human Arg-Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 2R36 "Crystal Structure Of Ni Human Arg-Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 2RN5 "Humal Insulin Mutant B31lys-B32arg" 53.19 32 100.00 100.00 3.35e-08 PDB 2TCI "X-Ray Crystallographic Studies On Hexameric Insulins In The Presence Of Helix-Stabilizing Agents, Thiocyanate, Methylparaben An" 53.19 30 100.00 100.00 4.63e-08 PDB 2VJZ "Crystal Structure Form Ultalente Insulin Microcrystals" 53.19 30 100.00 100.00 4.26e-08 PDB 2VK0 "Crystal Structure Form Ultalente Insulin Microcrystals" 53.19 30 100.00 100.00 4.26e-08 PDB 2W44 "Structure Deltaa1-a4 Insulin" 53.19 29 100.00 100.00 5.28e-08 PDB 2WC0 "Crystal Structure Of Human Insulin Degrading Enzyme In Complex With Iodinated Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 2WRU "Semi-synthetic Highly Active Analogue Of Human Insulin Nmealab26-dti-nh2" 53.19 26 100.00 100.00 8.95e-08 PDB 2WRV "Semi-Synthetic Highly Active Analogue Of Human Insulin Nmehisb26-Dti-Nh2" 53.19 26 100.00 100.00 8.95e-08 PDB 2WRW "Semi-Synthetic Highly Active Analogue Of Human Insulin D- Prob26-Dti-Nh2" 53.19 26 100.00 100.00 8.95e-08 PDB 2WRX "Semi-Synthetic Analogue Of Human Insulin Nmealab26-Insulin At Ph 3.0" 53.19 30 100.00 100.00 4.08e-08 PDB 2WS0 "Semi-Synthetic Analogue Of Human Insulin Nmealab26-Insulin At Ph 7.5" 53.19 30 100.00 100.00 4.08e-08 PDB 2WS1 "Semi-Synthetic Analogue Of Human Insulin Nmetyrb26-Insulin In Monomer Form" 53.19 30 100.00 100.00 4.08e-08 PDB 2WS6 "Semi-Synthetic Analogue Of Human Insulin Nmetyrb26-Insulin In Hexamer Form" 53.19 30 100.00 100.00 4.08e-08 PDB 2ZP6 "Crystal Structure Of Bovine Insulin (Hexameric Form)" 53.19 30 100.00 100.00 4.63e-08 PDB 2ZPP "Neutron Crystal Structure Of Cubic Insulin At Pd9" 53.19 30 100.00 100.00 4.63e-08 PDB 3AIY "R6 Human Insulin Hexamer (Symmetric), Nmr, Refined Average Structure" 53.19 30 100.00 100.00 4.26e-08 PDB 3E7Y "Structure Of Human Insulin" 53.19 29 100.00 100.00 5.28e-08 PDB 3E7Z "Structure Of Human Insulin" 53.19 29 100.00 100.00 5.28e-08 PDB 3EXX "Structure Of The T6 Human Insulin Derivative With Nickel At 1.35 A Resolution" 53.19 30 100.00 100.00 4.26e-08 PDB 3FHP "A Neutron Crystallographic Analysis Of A Porcine 2zn Insulin At 2.0 A Resolution" 53.19 30 100.00 100.00 4.63e-08 PDB 3FQ9 "Design Of An Insulin Analog With Enhanced Receptor-Binding Selectivity. Rationale, Structure, And Therapeutic Implications" 53.19 30 100.00 100.00 4.26e-08 PDB 3GKY "The Structural Basis Of An Er Stress-Associated Bottleneck In A Protein Folding Landscape" 53.19 30 100.00 100.00 4.63e-08 PDB 3I3Z "Human Insulin" 53.19 30 100.00 100.00 4.63e-08 PDB 3I40 "Human Insulin" 53.19 30 100.00 100.00 4.63e-08 PDB 3ILG "Crystal Structure Of Humnan Insulin Sr+2 Complex" 53.19 30 100.00 100.00 4.26e-08 PDB 3INC "Crystal Structure Of Human Insulin With Ni+2 Complex" 53.19 30 100.00 100.00 4.26e-08 PDB 3INS "Structure Of Insulin. Results Of Joint Neutron And X-ray Refinement" 53.19 30 100.00 100.00 4.63e-08 PDB 3IR0 "Crystal Structure Of Human Insulin Complexed With Cu+2 Metal Ion" 53.19 30 100.00 100.00 4.26e-08 PDB 3KQ6 "Enhancing The Therapeutic Properties Of A Protein By A Desig Binding Site, Structural Principles Of A Novel Long-Acting Analog" 53.19 30 100.00 100.00 4.26e-08 PDB 3MTH "X-Ray Crystallographic Studies On Hexameric Insulins In The Presence Of Helix-Stabilizing Agents, Thiocyanate, Methylparaben An" 53.19 30 100.00 100.00 4.63e-08 PDB 3P33 "Insulin Fibrillation Is The Janus Face Of Induced Fit. A Chiral Clamp Stabilizes The Native State At The Expense Of Activity" 53.19 30 100.00 100.00 4.26e-08 PDB 3Q6E "Human Insulin In Complex With Cucurbit[7]uril" 53.19 30 100.00 100.00 4.26e-08 PDB 3RTO "Acoustically Mounted Porcine Insulin Microcrystals Yield An X-Ray Sad Structure" 53.19 30 100.00 100.00 4.63e-08 PDB 3T2A "Tmao-Grown Cubic Insulin (Porcine)" 53.19 30 100.00 100.00 4.63e-08 PDB 3TT8 "Crystal Structure Analysis Of Cu Human Insulin Derivative" 53.19 30 100.00 100.00 4.26e-08 PDB 3U4N "A Novel Covalently Linked Insulin Dimer" 51.06 29 100.00 100.00 4.78e-07 PDB 3V19 "Forestalling Insulin Fibrillation By Insertion Of A Chiral Clamp Mechanism-Based Application Of Protein Engineering To Global H" 53.19 30 100.00 100.00 4.26e-08 PDB 3W11 "Insulin Receptor Ectodomain Construct Comprising Domains L1-cr In Complex With Human Insulin, Alpha-ct Peptide(704-719) And Fab" 53.19 30 100.00 100.00 4.26e-08 PDB 3W12 "Insulin Receptor Ectodomain Construct Comprising Domains L1-cr In Complex With High-affinity Insulin Analogue [d-pro-b26]-dti-n" 53.19 26 100.00 100.00 8.95e-08 PDB 3W13 "Insulin Receptor Ectodomain Construct Comprising Domains L1-cr In Complex With High-affinity Insulin Analogue [d-pro-b26]-dti-n" 53.19 26 100.00 100.00 8.95e-08 PDB 3W14 "Insulin Receptor Ectodomain Construct Comprising Domains L1,cr,l2, Fniii-1 And Alphact Peptide In Complex With Bovine Insulin A" 53.19 30 100.00 100.00 4.63e-08 PDB 3W7Y "0.92a Structure Of 2zn Human Insulin At 100k" 53.19 30 100.00 100.00 4.26e-08 PDB 3W7Z "1.15a Structure Of Human 2zn Insulin At 293k" 53.19 30 100.00 100.00 4.26e-08 PDB 3W80 "Crystal Structure Of Dodecamer Human Insulin With Double C-axis Length Of The Hexamer 2 Zn Insulin Cell" 53.19 30 100.00 100.00 4.26e-08 PDB 3ZQR "Nmepheb25 Insulin Analogue Crystal Structure" 51.06 30 100.00 100.00 2.61e-07 PDB 3ZU1 "Structure Of Lysb29(Nepsilon Omega-Carboxyheptadecanoyl) Des(B30) Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4A7E "X-Ray Crystal Structure Of Porcine Insulin Flash-Cooled At High Pressure" 53.19 30 100.00 100.00 4.63e-08 PDB 4AIY "R6 Human Insulin Hexamer (Symmetric), Nmr, 'green' Substate, Average Structure" 53.19 30 100.00 100.00 4.26e-08 PDB 4AJX "Ligand Controlled Assembly Of Hexamers, Dihexamers, And Linear Multihexamer Structures By An Engineered Acylated Insulin" 53.19 29 100.00 100.00 5.28e-08 PDB 4AJZ "Ligand Controlled Assembly Of Hexamers, Dihexamers, And Linear Multihexamer Structures By An Engineered Acylated Insulin" 53.19 29 100.00 100.00 5.28e-08 PDB 4AK0 "Ligand Controlled Assembly Of Hexamers, Dihexamers, And Linear Multihexamer Structures By An Engineered Acylated Insulin" 53.19 29 100.00 100.00 5.28e-08 PDB 4AKJ "Ligand Controlled Assembly Of Hexamers, Dihexamers, And Linear Multihexamer Structures By An Engineered Acylated Insulin" 53.19 29 100.00 100.00 5.28e-08 PDB 4BS3 "Bovin Insulin Structure Determined By In Situ Crystal Analysis And Sulfur-sad Phasing At Room Temperature" 53.19 30 100.00 100.00 4.63e-08 PDB 4E7T "The Structure Of T6 Bovine Insulin" 53.19 30 100.00 100.00 4.63e-08 PDB 4E7U "The Structure Of T3r3 Bovine Insulin" 53.19 30 100.00 100.00 4.63e-08 PDB 4E7V "The Structure Of R6 Bovine Insulin" 53.19 30 100.00 100.00 4.63e-08 PDB 4EWW "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4EWX "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4EWZ "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4EX0 "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4EX1 "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4EXX "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4EY1 "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4EY9 "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4EYD "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4EYN "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4EYP "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4F0N "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4F0O "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4F1A "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4F1B "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4F1C "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4F1D "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4F1F "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4F1G "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4F4T "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4F4V "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4F51 "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4F8F "Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4FG3 "Crystal Structure Analysis Of The Human Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4FKA "High Resolution Structure Of The Manganese Derivative Of Insulin" 53.19 30 100.00 100.00 4.26e-08 PDB 4GBC "Crystal Structure Of Aspart Insulin At Ph 6.5" 53.19 30 100.00 100.00 4.35e-08 PDB 4GBI "Crystal Structure Of Aspart Insulin At Ph 6.5" 53.19 30 100.00 100.00 4.35e-08 PDB 4GBK "Crystal Structure Of Aspart Insulin At Ph 8.5" 53.19 30 100.00 100.00 4.35e-08 PDB 4GBL "Crystal Structure Of Aspart Insulin At Ph 8.5" 53.19 30 100.00 100.00 4.35e-08 PDB 4GBN "Crystal Structure Of Aspart Insulin At Ph 6.5" 53.19 30 100.00 100.00 4.35e-08 PDB 4I5Y "Insulin Protein Crystallization Via Langmuir-blodgett" 53.19 30 100.00 100.00 4.63e-08 PDB 4I5Z "Insulin Protein Crystallization Via Langmuir-blodgett" 53.19 30 100.00 100.00 4.63e-08 PDB 4IDW "Polycrystalline T6 Bovine Insulin: Anisotropic Lattice Evolution And Novel Structure Refinement Strategy" 53.19 30 100.00 100.00 4.63e-08 PDB 4IHN "High Resolution Insulin By Langmuir-blodgett Modified Hanging Drop Vapour Diffusion" 53.19 30 100.00 100.00 4.63e-08 PDB 4INS "The Structure Of 2zn Pig Insulin Crystals At 1.5 Angstroms Resolution" 53.19 30 100.00 100.00 4.63e-08 PDB 4IYD "Insulin Glargine Crystal Structure 1" 53.19 29 100.00 100.00 5.28e-08 PDB 4IYF "Insulin Glargine Crystal Structure 2" 53.19 29 100.00 100.00 5.28e-08 PDB 4M4F "Radiation Damage Study Of Cu T6-insulin - 0.01 Mgy" 53.19 30 100.00 100.00 4.63e-08 PDB 4M4H "Radiation Damage Study Of Cu T6-insulin - 0.06 Mgy" 53.19 30 100.00 100.00 4.63e-08 PDB 4M4I "Radiation Damage Study Of Cu T6-insulin - 0.12 Mgy" 53.19 30 100.00 100.00 4.63e-08 PDB 4M4J "Radiation Damage Study Of Cu T6-insulin - 0.30 Mgy" 53.19 30 100.00 100.00 4.63e-08 PDB 4M4L "The Structure Of Cu T6 Bovine Insulin" 53.19 30 100.00 100.00 4.63e-08 PDB 4M4M "The Structure Of Ni T6 Bovine Insulin" 53.19 30 100.00 100.00 4.63e-08 PDB 4OGA "Insulin In Complex With Site 1 Of The Human Insulin Receptor" 53.19 30 100.00 100.00 4.26e-08 PDB 4RXW "Crystal Structure Of The Cobalt Human Insulin Derivative" 53.19 30 100.00 100.00 4.26e-08 PDB 4UNE "Human Insulin B26phe Mutant Crystal Structure" 53.19 30 100.00 100.00 4.58e-08 PDB 4UNG "Human Insulin B26asn Mutant Crystal Structure" 53.19 30 100.00 100.00 3.95e-08 PDB 4XC4 "Insulin Co-crystallizes In The Presence Of It Beta-cell Chaperone Sulfatide" 53.19 30 100.00 100.00 4.26e-08 PDB 5AIY "R6 Human Insulin Hexamer (Symmetric), Nmr, 'red' Substate, Average Structure" 53.19 30 100.00 100.00 4.26e-08 PDB 5CNY "Crystal Structure Of Human Zinc Insulin At Ph 5.5" 53.19 30 100.00 100.00 4.26e-08 PDB 5CO2 "Crystalization Of Human Zinc Insulin At Ph 5.5" 53.19 30 100.00 100.00 4.26e-08 PDB 5CO6 "Crystal Structure Of Human Zinc Insulin At Ph 6.5" 53.19 30 100.00 100.00 4.26e-08 PDB 5CO9 "Crystal Structure Of Human Zinc Insulin At Ph 6.5" 53.19 30 100.00 100.00 4.26e-08 PDB 7INS "Structure Of Porcine Insulin Cocrystallized With Clupeine Z" 53.19 30 100.00 100.00 4.63e-08 PDB 9INS "Monovalent Cation Binding In Cubic Insulin Crystals" 53.19 30 100.00 100.00 4.63e-08 EMBL CAA08766 "insulin [Homo sapiens]" 53.19 59 100.00 100.00 1.29e-08 GB AAA72171 "insulin B chain [synthetic construct]" 53.19 31 100.00 100.00 3.62e-08 GB AAA72917 "insulin beta chain, partial [synthetic construct]" 53.19 30 100.00 100.00 4.26e-08 GB AAB25817 "insulin B-chain, partial [Equus przewalskii]" 53.19 30 100.00 100.00 4.63e-08 GB AAI48489 "INS-IGF2 readthrough transcript, partial [synthetic construct]" 53.19 200 100.00 100.00 1.22e-09 GB AAI53084 "INS-IGF2 readthrough transcript [synthetic construct]" 53.19 200 100.00 100.00 1.22e-09 PRF 0510475A "insulin B,24/25-Leu" 53.19 30 100.00 100.00 4.26e-08 PRF 550086A insulin 97.87 51 100.00 100.00 1.40e-23 PRF 560164A insulin 97.87 51 97.83 97.83 8.00e-23 PRF 580107B insulin 97.87 50 97.83 97.83 5.15e-21 PRF 600165A insulin 97.87 51 100.00 100.00 1.48e-23 REF NP_001035835 "insulin, isoform 2 precursor [Homo sapiens]" 53.19 200 100.00 100.00 1.22e-09 REF XP_004050477 "PREDICTED: insulin isoform 3 [Gorilla gorilla gorilla]" 53.19 200 100.00 100.00 1.22e-09 REF XP_006766071 "PREDICTED: insulin-like [Myotis davidii]" 53.19 128 100.00 100.00 1.44e-08 REF XP_008576869 "PREDICTED: insulin, isoform 2-like [Galeopterus variegatus]" 53.19 200 100.00 100.00 1.22e-09 REF XP_008697844 "PREDICTED: insulin, isoform 2-like [Ursus maritimus]" 53.19 202 100.00 100.00 1.24e-09 SP F8WCM5 "RecName: Full=Insulin, isoform 2; AltName: Full=INS-IGF2 readthrough transcript protein" 53.19 200 100.00 100.00 1.22e-09 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $D-AlaB26_DTI-amide human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $D-AlaB26_DTI-amide 'chemically synthesized' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $D-AlaB26_DTI-amide 4 mM . stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer . _Model . _Field_strength . _Details . save_ ####################### # Sample conditions # ####################### save_sample_conditions_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH* 1.9 0.2 na temperature 298 1 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis DSS H 1 'methyl protons' ppm 0.0 internal direct . . 1.0 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_one _Chem_shift_reference_set_label $chemical_shift_reference_one _Mol_system_component_name [D-AlaB26]DTI-amide_A-chain _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 GLY HA2 H 3.992 . . 2 . 2 ILE H H 8.495 . . 3 . 2 ILE HA H 3.940 . . 4 . 2 ILE HB H 1.325 . . 5 . 2 ILE HG12 H 1.224 . . 6 . 2 ILE HG13 H 1.151 . . 7 . 2 ILE HD1 H 0.606 . . 8 . 2 ILE HG2 H 0.739 . . 9 . 3 VAL H H 8.098 . . 10 . 3 VAL HA H 3.629 . . 11 . 3 VAL HB H 1.906 . . 12 . 3 VAL HG1 H 0.864 . . 13 . 4 GLU H H 8.094 . . 14 . 4 GLU HA H 4.221 . . 15 . 4 GLU HB2 H 2.091 . . 16 . 4 GLU HB3 H 2.091 . . 17 . 4 GLU HG2 H 2.500 . . 18 . 4 GLU HG3 H 2.500 . . 19 . 5 GLN H H 8.229 . . 20 . 5 GLN HA H 4.054 . . 21 . 5 GLN HB2 H 2.099 . . 22 . 5 GLN HB3 H 2.099 . . 23 . 5 GLN HG2 H 2.483 . . 24 . 5 GLN HG3 H 2.399 . . 25 . 6 CYS H H 8.280 . . 26 . 6 CYS HA H 4.878 . . 27 . 6 CYS HB2 H 2.856 . . 28 . 6 CYS HB3 H 3.320 . . 29 . 7 CYS H H 8.260 . . 30 . 7 CYS HA H 4.830 . . 31 . 7 CYS HB2 H 3.302 . . 32 . 7 CYS HB3 H 3.760 . . 33 . 8 THR H H 8.239 . . 34 . 8 THR HA H 4.029 . . 35 . 8 THR HB H 4.372 . . 36 . 8 THR HG2 H 1.222 . . 37 . 9 SER H H 7.441 . . 38 . 9 SER HA H 4.763 . . 39 . 9 SER HB2 H 3.874 . . 40 . 9 SER HB3 H 4.028 . . 41 . 10 ILE H H 7.808 . . 42 . 10 ILE HA H 4.351 . . 43 . 10 ILE HB H 1.540 . . 44 . 10 ILE HG12 H 1.072 . . 45 . 10 ILE HG13 H 0.434 . . 46 . 10 ILE HD1 H 0.506 . . 47 . 10 ILE HG2 H 0.615 . . 48 . 11 CYS H H 9.676 . . 49 . 12 SER H H 8.739 . . 50 . 12 SER HA H 4.588 . . 51 . 12 SER HB2 H 4.292 . . 52 . 12 SER HB3 H 3.986 . . 53 . 13 LEU H H 8.610 . . 54 . 13 LEU HA H 3.845 . . 55 . 13 LEU HB2 H 1.332 . . 56 . 13 LEU HB3 H 1.397 . . 57 . 13 LEU HG H 1.332 . . 58 . 13 LEU HD1 H 0.785 . . 59 . 13 LEU HD2 H 0.723 . . 60 . 14 TYR H H 7.471 . . 61 . 14 TYR HA H 4.142 . . 62 . 14 TYR HB2 H 2.977 . . 63 . 14 TYR HB3 H 2.889 . . 64 . 14 TYR HD1 H 7.036 . . 65 . 14 TYR HE1 H 6.816 . . 66 . 15 GLN H H 7.541 . . 67 . 15 GLN HA H 3.967 . . 68 . 15 GLN HB2 H 2.017 . . 69 . 15 GLN HG2 H 2.383 . . 70 . 15 GLN HG3 H 2.383 . . 71 . 15 GLN HE21 H 7.474 . . 72 . 16 LEU H H 8.075 . . 73 . 16 LEU HA H 4.159 . . 74 . 16 LEU HB2 H 1.919 . . 75 . 16 LEU HB3 H 1.584 . . 76 . 16 LEU HG H 1.731 . . 77 . 16 LEU HD1 H 0.799 . . 78 . 16 LEU HD2 H 0.834 . . 79 . 17 GLU H H 8.064 . . 80 . 17 GLU HA H 4.162 . . 81 . 17 GLU HB2 H 2.035 . . 82 . 17 GLU HB3 H 2.110 . . 83 . 17 GLU HG2 H 2.311 . . 84 . 17 GLU HG3 H 2.552 . . 85 . 18 ASN H H 7.417 . . 86 . 18 ASN HA H 4.462 . . 87 . 18 ASN HB2 H 2.533 . . 88 . 18 ASN HB3 H 2.598 . . 89 . 18 ASN HD21 H 7.147 . . 90 . 18 ASN HD22 H 6.502 . . 91 . 19 TYR H H 7.894 . . 92 . 19 TYR HA H 4.463 . . 93 . 19 TYR HB2 H 2.994 . . 94 . 19 TYR HB3 H 3.300 . . 95 . 19 TYR HD1 H 7.303 . . 96 . 19 TYR HE1 H 6.747 . . 97 . 20 CYS H H 7.448 . . 98 . 20 CYS HA H 4.774 . . 99 . 20 CYS HB2 H 3.240 . . 100 . 20 CYS HB3 H 2.837 . . 101 . 21 ASN H H 8.256 . . 102 . 21 ASN HA H 4.703 . . 103 . 21 ASN HB2 H 2.861 . . 104 . 21 ASN HB3 H 2.758 . . 105 . 21 ASN HD21 H 6.700 . . 106 . 21 ASN HD22 H 7.509 . . 107 . 22 PHE HA H 4.252 . . 108 . 22 PHE HB2 H 3.144 . . 109 . 22 PHE HB3 H 3.144 . . 110 . 22 PHE HD1 H 7.197 . . 111 . 22 PHE HE1 H 7.334 . . 112 . 22 PHE HZ H 7.233 . . 113 . 23 VAL H H 8.134 . . 114 . 23 VAL HA H 4.114 . . 115 . 23 VAL HB H 1.886 . . 116 . 23 VAL HG1 H 0.841 . . 117 . 23 VAL HG2 H 0.841 . . 118 . 24 ASN H H 8.453 . . 119 . 24 ASN HA H 4.705 . . 120 . 24 ASN HB2 H 2.707 . . 121 . 24 ASN HB3 H 2.707 . . 122 . 24 ASN HD21 H 7.324 . . 123 . 24 ASN HD22 H 6.897 . . 124 . 25 GLN H H 8.403 . . 125 . 25 GLN HA H 4.480 . . 126 . 25 GLN HB2 H 2.128 . . 127 . 25 GLN HB3 H 1.899 . . 128 . 25 GLN HG2 H 2.252 . . 129 . 25 GLN HG3 H 2.252 . . 130 . 25 GLN HE21 H 7.324 . . 131 . 25 GLN HE22 H 6.782 . . 132 . 26 HIS H H 8.617 . . 133 . 26 HIS HA H 4.440 . . 134 . 26 HIS HB2 H 3.247 . . 135 . 26 HIS HB3 H 3.543 . . 136 . 26 HIS HE1 H 8.564 . . 137 . 26 HIS HD1 H 7.381 . . 138 . 27 LEU H H 8.967 . . 139 . 27 LEU HA H 4.497 . . 140 . 27 LEU HB2 H 1.757 . . 141 . 27 LEU HG H 1.600 . . 142 . 27 LEU HD1 H 0.882 . . 143 . 27 LEU HD2 H 0.721 . . 144 . 28 CYS H H 8.312 . . 145 . 28 CYS HA H 4.962 . . 146 . 28 CYS HB2 H 3.211 . . 147 . 28 CYS HB3 H 2.951 . . 148 . 29 GLY H H 9.219 . . 149 . 29 GLY HA2 H 3.980 . . 150 . 29 GLY HA3 H 3.823 . . 151 . 30 SER H H 9.056 . . 152 . 30 SER HA H 4.112 . . 153 . 30 SER HB2 H 3.890 . . 154 . 30 SER HB3 H 3.890 . . 155 . 31 HIS H H 7.999 . . 156 . 31 HIS HA H 4.514 . . 157 . 31 HIS HB2 H 3.576 . . 158 . 31 HIS HB3 H 3.305 . . 159 . 31 HIS HE1 H 8.671 . . 160 . 31 HIS HD1 H 7.458 . . 161 . 32 LEU H H 7.060 . . 162 . 32 LEU HA H 4.002 . . 163 . 32 LEU HB2 H 1.892 . . 164 . 32 LEU HB3 H 1.233 . . 165 . 32 LEU HG H 1.319 . . 166 . 32 LEU HD1 H 0.930 . . 167 . 32 LEU HD2 H 0.930 . . 168 . 33 VAL H H 7.176 . . 169 . 33 VAL HA H 3.326 . . 170 . 33 VAL HB H 2.047 . . 171 . 33 VAL HG1 H 0.954 . . 172 . 33 VAL HG2 H 0.954 . . 173 . 34 GLU H H 7.938 . . 174 . 34 GLU HA H 4.090 . . 175 . 34 GLU HB2 H 2.083 . . 176 . 34 GLU HB3 H 2.083 . . 177 . 34 GLU HG2 H 2.533 . . 178 . 34 GLU HG3 H 2.169 . . 179 . 35 ALA H H 7.748 . . 180 . 35 ALA HA H 4.081 . . 181 . 35 ALA HB H 1.485 . . 182 . 36 LEU H H 8.063 . . 183 . 36 LEU HA H 3.903 . . 184 . 36 LEU HG H 1.490 . . 185 . 36 LEU HD1 H 0.659 . . 186 . 36 LEU HD2 H 0.465 . . 187 . 37 TYR H H 8.173 . . 188 . 37 TYR HA H 4.256 . . 189 . 37 TYR HB2 H 3.130 . . 190 . 37 TYR HB3 H 3.130 . . 191 . 37 TYR HD1 H 7.112 . . 192 . 37 TYR HE1 H 6.756 . . 193 . 38 LEU H H 7.855 . . 194 . 38 LEU HA H 4.081 . . 195 . 38 LEU HB2 H 1.921 . . 196 . 38 LEU HB3 H 1.648 . . 197 . 38 LEU HG H 1.833 . . 198 . 38 LEU HD1 H 0.916 . . 199 . 38 LEU HD2 H 0.916 . . 200 . 39 VAL H H 8.511 . . 201 . 39 VAL HA H 3.847 . . 202 . 39 VAL HB H 2.096 . . 203 . 39 VAL HG1 H 1.011 . . 204 . 39 VAL HG2 H 0.872 . . 205 . 40 CYS H H 8.724 . . 206 . 40 CYS HA H 4.777 . . 207 . 40 CYS HB2 H 3.215 . . 208 . 40 CYS HB3 H 2.931 . . 209 . 41 GLY H H 7.721 . . 210 . 41 GLY HA2 H 3.935 . . 211 . 41 GLY HA3 H 3.935 . . 212 . 42 GLU H H 8.403 . . 213 . 42 GLU HA H 4.225 . . 214 . 42 GLU HB2 H 2.178 . . 215 . 42 GLU HB3 H 2.086 . . 216 . 42 GLU HG2 H 2.506 . . 217 . 42 GLU HG3 H 2.506 . . 218 . 43 ARG H H 7.982 . . 219 . 43 ARG HA H 4.208 . . 220 . 43 ARG HB2 H 1.947 . . 221 . 43 ARG HB3 H 1.947 . . 222 . 43 ARG HG2 H 1.756 . . 223 . 43 ARG HG3 H 1.756 . . 224 . 44 GLY H H 7.692 . . 225 . 44 GLY HA2 H 3.810 . . 226 . 44 GLY HA3 H 3.973 . . 227 . 45 PHE H H 7.719 . . 228 . 45 PHE HA H 4.839 . . 229 . 45 PHE HB2 H 2.935 . . 230 . 45 PHE HB3 H 3.106 . . 231 . 45 PHE HD1 H 6.939 . . 232 . 45 PHE HE1 H 7.069 . . 233 . 46 PHE H H 8.362 . . 234 . 46 PHE HA H 4.532 . . 235 . 46 PHE HB2 H 3.098 . . 236 . 46 PHE HB3 H 3.098 . . 237 . 46 PHE HD1 H 7.233 . . 238 . 46 PHE HE1 H 7.333 . . 239 . 47 ALA H H 8.015 . . 240 . 47 ALA HA H 4.216 . . 241 . 47 ALA HB H 1.164 . . stop_ save_