data_1693 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Comparison of Helix Stability in Wild-type and Mutant LamB Signal Sequences ; _BMRB_accession_number 1693 _BMRB_flat_file_name bmr1693.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bruch Martha D. . 2 Gierasch Lila M. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 41 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-15 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Bruch, Martha D., Gierasch, Lila M., "Comparison of Helix Stability in Wild-type and Mutant LamB Signal Sequences," J. Biol. Chem. 265 (7), 3851-3858 (1990). ; _Citation_title 'Comparison of Helix Stability in Wild-type and Mutant LamB Signal Sequences' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bruch Martha D. . 2 Gierasch Lila M. . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_volume 265 _Journal_issue 7 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 3851 _Page_last 3858 _Year 1990 _Details . save_ ################################## # Molecular system description # ################################## save_system_synthesized_signal_peptide _Saveframe_category molecular_system _Mol_system_name 'synthesized signal peptide' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'synthesized signal peptide' $synthesized_signal_peptide stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_synthesized_signal_peptide _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'synthesized signal peptide' _Name_variant 'lamB protein signal sequence deletion 78' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 21 _Mol_residue_sequence ; MMITLRKLPVAAGVMSAQAM A ; loop_ _Residue_seq_code _Residue_label 1 MET 2 MET 3 ILE 4 THR 5 LEU 6 ARG 7 LYS 8 LEU 9 PRO 10 VAL 11 ALA 12 ALA 13 GLY 14 VAL 15 MET 16 SER 17 ALA 18 GLN 19 ALA 20 MET 21 ALA stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 1999-12-06 save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain $synthesized_signal_peptide 'E. coli' 562 Bacteria . Escherichia coli generic stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $synthesized_signal_peptide 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 4 . na temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label CF3CD2OD H . CH2 ppm 3.88 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'synthesized signal peptide' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 MET HA H 4.12 . 1 2 . 2 MET H H 8.57 . 1 3 . 2 MET HA H 4.59 . 1 4 . 3 ILE H H 8.03 . 1 5 . 3 ILE HA H 4.21 . 1 6 . 4 THR H H 7.78 . 1 7 . 4 THR HA H 4.34 . 1 8 . 5 LEU H H 7.86 . 1 9 . 5 LEU HA H 4.34 . 1 10 . 6 ARG H H 7.91 . 1 11 . 6 ARG HA H 4.27 . 1 12 . 7 LYS H H 7.89 . 1 13 . 7 LYS HA H 4.34 . 1 14 . 8 LEU H H 7.82 . 1 15 . 8 LEU HA H 4.6 . 1 16 . 9 PRO HA H 4.46 . 1 17 . 10 VAL H H 7.53 . 1 18 . 10 VAL HA H 3.95 . 1 19 . 11 ALA H H 7.92 . 1 20 . 11 ALA HA H 4.16 . 1 21 . 12 ALA H H 7.96 . 1 22 . 12 ALA HA H 4.16 . 1 23 . 13 GLY H H 8.07 . 1 24 . 13 GLY HA2 H 3.92 . 2 25 . 13 GLY HA3 H 3.86 . 2 26 . 14 VAL H H 7.87 . 1 27 . 14 VAL HA H 3.88 . 1 28 . 15 MET H H 8.22 . 1 29 . 15 MET HA H 4.35 . 1 30 . 16 SER H H 8.01 . 1 31 . 16 SER HA H 4.32 . 1 32 . 17 ALA H H 7.94 . 1 33 . 17 ALA HA H 4.23 . 1 34 . 18 GLN H H 8.05 . 1 35 . 18 GLN HA H 4.18 . 1 36 . 19 ALA H H 7.88 . 1 37 . 19 ALA HA H 4.28 . 1 38 . 20 MET H H 7.78 . 1 39 . 20 MET HA H 4.47 . 1 40 . 21 ALA H H 7.79 . 1 41 . 21 ALA HA H 4.34 . 1 stop_ save_