data_15274 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; NMR chemical shift assignments for Arf-binding domain of Hdm2 ; _BMRB_accession_number 15274 _BMRB_flat_file_name bmr15274.str _Entry_type original _Submission_date 2007-05-31 _Accession_date 2007-05-31 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'NMR chemical shift assignments for Arf-binding domain of Hdm2 (residues 210-304)' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Sivakolundu Sivashankar G. . 2 Kriwacki Richard W. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 91 "13C chemical shifts" 276 "15N chemical shifts" 91 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2008-11-21 update BMRB 'update entry citation' 2008-10-28 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Intrinsically Unstructured Domains of Arf and Hdm2 Form Bimolecular Oligomeric Structures In Vitro and In Vivo' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 18809412 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Sivakolundu Sivashankar G. . 2 Nourse Amanda . . 3 Moshiach Simon . . 4 Bothner Brian . . 5 Ashley Chimere . . 6 Satumba John . . 7 Lahti Jill . . 8 Kriwacki Richard W. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_name_full 'Journal of Molecular Biology' _Journal_volume 384 _Journal_issue 1 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 240 _Page_last 254 _Year 2008 _Details . loop_ _Keyword arf hdm2 stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name Hdm2-ABD _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label Hdm2-ABD $Hdm2-ABD stop_ _System_molecular_weight 10913 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Hdm2-ABD _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Hdm2-ABD _Molecular_mass . _Mol_thiol_state 'not present' loop_ _Biological_function 'Transcriptional regulator and E3 ubiquitin ligase of p53' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 99 _Mol_residue_sequence ; GSHMSSSSESTGTPSNPDLD AGVSEHSGDWLDQDSVSDQF SVEFEVESLDSEDYSLSEEG QELSDEDDEVYQVTVYQAGE SDTDSFEEDPEISLADYWK ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 SER 3 HIS 4 MET 5 SER 6 SER 7 SER 8 SER 9 GLU 10 SER 11 THR 12 GLY 13 THR 14 PRO 15 SER 16 ASN 17 PRO 18 ASP 19 LEU 20 ASP 21 ALA 22 GLY 23 VAL 24 SER 25 GLU 26 HIS 27 SER 28 GLY 29 ASP 30 TRP 31 LEU 32 ASP 33 GLN 34 ASP 35 SER 36 VAL 37 SER 38 ASP 39 GLN 40 PHE 41 SER 42 VAL 43 GLU 44 PHE 45 GLU 46 VAL 47 GLU 48 SER 49 LEU 50 ASP 51 SER 52 GLU 53 ASP 54 TYR 55 SER 56 LEU 57 SER 58 GLU 59 GLU 60 GLY 61 GLN 62 GLU 63 LEU 64 SER 65 ASP 66 GLU 67 ASP 68 ASP 69 GLU 70 VAL 71 TYR 72 GLN 73 VAL 74 THR 75 VAL 76 TYR 77 GLN 78 ALA 79 GLY 80 GLU 81 SER 82 ASP 83 THR 84 ASP 85 SER 86 PHE 87 GLU 88 GLU 89 ASP 90 PRO 91 GLU 92 ILE 93 SER 94 LEU 95 ALA 96 ASP 97 TYR 98 TRP 99 LYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value DBJ BAB11975 "MDM2 [Canis lupus familiaris]" 95.96 487 98.95 100.00 1.16e-53 DBJ BAC78209 "double minute 2 protein MDM2 [Felis catus]" 95.96 491 98.95 100.00 9.19e-54 DBJ BAF83030 "unnamed protein product [Homo sapiens]" 95.96 491 100.00 100.00 2.56e-54 DBJ BAJ17752 "Mdm2 p53 binding protein homolog [synthetic construct]" 95.96 497 100.00 100.00 2.43e-54 EMBL CAA78055 "p53 associated [Homo sapiens]" 95.96 491 100.00 100.00 2.56e-54 EMBL CAH89564 "hypothetical protein [Pongo abelii]" 95.96 497 97.89 98.95 8.27e-53 EMBL CAP16704 "MDM2 protein [Homo sapiens]" 95.96 522 100.00 100.00 9.52e-54 EMBL CAP16705 "MDM2 protein [Homo sapiens]" 95.96 455 100.00 100.00 1.15e-54 EMBL CAP16729 "MDM2 protein [Homo sapiens]" 91.92 305 100.00 100.00 2.08e-51 GB AAA60568 "p53 associated [Homo sapiens]" 95.96 491 100.00 100.00 2.56e-54 GB AAA75514 "mdm2-A [Homo sapiens]" 82.83 296 100.00 100.00 1.46e-46 GB AAA75516 "mdm2-C [Homo sapiens]" 82.83 321 100.00 100.00 1.89e-46 GB AAF28866 "double minute 2 protein [Equus caballus]" 95.96 491 98.95 100.00 7.69e-54 GB AAF42995 "MDM2-a1 [Homo sapiens]" 54.55 265 98.15 98.15 9.60e-24 PRF 1814460A "p53-associated protein" 95.96 491 100.00 100.00 2.56e-54 REF NP_001009346 "E3 ubiquitin-protein ligase Mdm2 [Felis catus]" 95.96 491 98.95 100.00 9.19e-54 REF NP_001092577 "E3 ubiquitin-protein ligase Mdm2 [Bos taurus]" 95.96 492 98.95 100.00 7.99e-54 REF NP_001124685 "E3 ubiquitin-protein ligase Mdm2 [Pongo abelii]" 95.96 497 97.89 98.95 8.27e-53 REF NP_001138811 "E3 ubiquitin-protein ligase Mdm2 isoform h [Homo sapiens]" 95.96 442 100.00 100.00 1.20e-54 REF NP_001138812 "E3 ubiquitin-protein ligase Mdm2 isoform i [Homo sapiens]" 54.55 295 98.15 98.15 8.48e-24 SP P56950 "RecName: Full=E3 ubiquitin-protein ligase Mdm2; AltName: Full=Double minute 2 protein; Short=Cdm2; AltName: Full=p53-binding pr" 95.96 487 98.95 100.00 1.08e-53 SP P56951 "RecName: Full=E3 ubiquitin-protein ligase Mdm2; AltName: Full=Double minute 2 protein; Short=Edm2; AltName: Full=p53-binding pr" 95.96 491 98.95 100.00 7.69e-54 SP Q00987 "RecName: Full=E3 ubiquitin-protein ligase Mdm2; AltName: Full=Double minute 2 protein; Short=Hdm2; AltName: Full=Oncoprotein Md" 95.96 491 100.00 100.00 2.56e-54 SP Q7YRZ8 "RecName: Full=E3 ubiquitin-protein ligase Mdm2; AltName: Full=Double minute 2 protein; AltName: Full=p53-binding protein Mdm2" 95.96 491 98.95 100.00 9.19e-54 TPG DAA29805 "TPA: MDM2-like protein [Bos taurus]" 95.96 492 98.95 100.00 7.91e-54 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Details $Hdm2-ABD Human 9606 Eukaryota Metazoa Homo sapiens 'Central domain comprising residues 210 to 304 of Hdm2' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Hdm2-ABD 'recombinant technology' . Escherichia coli BL21(DE3) pET28a stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '1 mM Hdm2-ABD in 10 mM sodium phosphate and 10 mM NaCl at pH6' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Hdm2-ABD 1 mM '[U-98% 13C; U-98% 15N]' 'sodium phosphate' 10 mM 'natural abundance' NaCl 10 mM 'natural abundance' H2O 95 % 'natural abundance' D2O 5 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_Felix _Saveframe_category software _Name FELIX _Version . loop_ _Vendor _Address _Electronic_address 'Accelrys Software Inc.' . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details 'Equiped with a TCI cryoprobe' save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details 'Equiped with a TCI cryoprobe' save_ save_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HNCA_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_3D_HNCO_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HCACO_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCACO' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details '10 mM sodium phosphate' loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.02 . M pH 6 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details 'referenced using tsp' loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio TSP C 13 'methyl protons' ppm 0 external indirect . . . . TSP H 1 'methyl protons' ppm 0 external direct . . . 1 TSP N 15 'methyl protons' ppm 0 external indirect . . . . stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $Felix stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D CBCA(CO)NH' '3D HNCACB' '3D HNCA' '3D HN(CO)CA' '3D HNCO' '3D HCACO' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name Hdm2-ABD _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 210 5 SER H H 8.45 . . 2 210 5 SER CA C 58.17 . . 3 210 5 SER CB C 63.95 . . 4 210 5 SER N N 117.58 . . 5 211 6 SER CA C 58.35 . . 6 211 6 SER CB C 63.95 . . 7 212 7 SER H H 8.47 . . 8 212 7 SER C C 176.72 . . 9 212 7 SER CA C 58.46 . . 10 212 7 SER CB C 63.95 . . 11 212 7 SER N N 119.08 . . 12 213 8 SER H H 8.41 . . 13 213 8 SER C C 176.89 . . 14 213 8 SER CA C 58.69 . . 15 213 8 SER CB C 63.95 . . 16 213 8 SER N N 118.98 . . 17 214 9 GLU H H 8.40 . . 18 214 9 GLU C C 174.85 . . 19 214 9 GLU CA C 56.75 . . 20 214 9 GLU CB C 30.06 . . 21 214 9 GLU N N 123.60 . . 22 215 10 SER H H 8.39 . . 23 215 10 SER C C 176.55 . . 24 215 10 SER CA C 58.35 . . 25 215 10 SER CB C 63.95 . . 26 215 10 SER N N 117.76 . . 27 216 11 THR H H 8.23 . . 28 216 11 THR C C 176.36 . . 29 216 11 THR CA C 62.00 . . 30 216 11 THR CB C 69.74 . . 31 216 11 THR N N 116.60 . . 32 217 12 GLY H H 8.41 . . 33 217 12 GLY C C 177.63 . . 34 217 12 GLY CA C 45.17 . . 35 217 12 GLY N N 112.20 . . 36 218 13 THR H H 8.15 . . 37 218 13 THR C C 178.59 . . 38 218 13 THR CA C 59.77 . . 39 218 13 THR CB C 69.74 . . 40 218 13 THR N N 117.76 . . 41 219 14 PRO C C 175.25 . . 42 219 14 PRO CA C 62.96 . . 43 219 14 PRO CB C 34.52 . . 44 220 15 SER H H 8.64 . . 45 220 15 SER C C 177.55 . . 46 220 15 SER CA C 58.58 . . 47 220 15 SER CB C 63.95 . . 48 220 15 SER N N 117.89 . . 49 221 16 ASN H H 8.35 . . 50 221 16 ASN C C 178.02 . . 51 221 16 ASN N N 126.41 . . 52 222 17 PRO C C 174.77 . . 53 222 17 PRO CA C 63.71 . . 54 222 17 PRO CB C 32.29 . . 55 223 18 ASP H H 8.29 . . 56 223 18 ASP C C 175.01 . . 57 223 18 ASP CA C 54.64 . . 58 223 18 ASP CB C 40.76 . . 59 223 18 ASP N N 120.52 . . 60 224 19 LEU H H 7.98 . . 61 224 19 LEU C C 174.17 . . 62 224 19 LEU CA C 55.44 . . 63 224 19 LEU CB C 42.55 . . 64 224 19 LEU N N 123.19 . . 65 225 20 ASP H H 8.26 . . 66 225 20 ASP C C 175.35 . . 67 225 20 ASP CA C 54.46 . . 68 225 20 ASP CB C 41.21 . . 69 225 20 ASP N N 122.03 . . 70 226 21 ALA H H 8.18 . . 71 226 21 ALA C C 173.08 . . 72 226 21 ALA CA C 52.93 . . 73 226 21 ALA CB C 18.92 . . 74 226 21 ALA N N 125.78 . . 75 227 22 GLY H H 8.42 . . 76 227 22 GLY C C 177.13 . . 77 227 22 GLY CA C 45.46 . . 78 227 22 GLY N N 108.92 . . 79 228 23 VAL H H 7.93 . . 80 228 23 VAL C C 175.10 . . 81 228 23 VAL CA C 62.34 . . 82 228 23 VAL CB C 32.74 . . 83 228 23 VAL N N 120.28 . . 84 229 24 SER H H 8.40 . . 85 229 24 SER C C 176.98 . . 86 229 24 SER CA C 58.29 . . 87 229 24 SER CB C 63.95 . . 88 229 24 SER N N 120.32 . . 89 230 25 GLU H H 8.43 . . 90 230 25 GLU C C 175.35 . . 91 230 25 GLU CA C 56.75 . . 92 230 25 GLU CB C 30.06 . . 93 230 25 GLU N N 123.69 . . 94 231 26 HIS H H 8.45 . . 95 231 26 HIS C C 177.11 . . 96 231 26 HIS CA C 55.04 . . 97 231 26 HIS CB C 29.17 . . 98 231 26 HIS N N 120.17 . . 99 232 27 SER H H 8.38 . . 100 232 27 SER C C 176.51 . . 101 232 27 SER CA C 58.56 . . 102 232 27 SER CB C 63.95 . . 103 232 27 SER N N 118.72 . . 104 233 28 GLY H H 8.51 . . 105 233 28 GLY C C 177.55 . . 106 233 28 GLY CA C 45.46 . . 107 233 28 GLY N N 111.93 . . 108 234 29 ASP H H 8.21 . . 109 234 29 ASP C C 175.42 . . 110 234 29 ASP CA C 54.41 . . 111 234 29 ASP CB C 40.96 . . 112 234 29 ASP N N 121.60 . . 113 235 30 TRP H H 8.04 . . 114 235 30 TRP C C 175.60 . . 115 235 30 TRP CA C 57.49 . . 116 235 30 TRP CB C 29.17 . . 117 235 30 TRP N N 122.42 . . 118 236 31 LEU H H 8.14 . . 119 236 31 LEU C C 174.94 . . 120 236 31 LEU CA C 54.92 . . 121 236 31 LEU CB C 42.61 . . 122 236 31 LEU N N 122.37 . . 123 237 32 ASP H H 7.89 . . 124 237 32 ASP C C 175.19 . . 125 237 32 ASP CA C 54.24 . . 126 237 32 ASP CB C 40.76 . . 127 237 32 ASP N N 125.26 . . 128 238 33 GLN H H 8.29 . . 129 238 33 GLN C C 175.57 . . 130 238 33 GLN CA C 56.12 . . 131 238 33 GLN CB C 29.62 . . 132 238 33 GLN N N 121.90 . . 133 239 34 ASP H H 8.43 . . 134 239 34 ASP C C 175.14 . . 135 239 34 ASP CA C 54.76 . . 136 239 34 ASP CB C 41.20 . . 137 239 34 ASP N N 122.18 . . 138 240 35 SER H H 8.16 . . 139 240 35 SER C C 176.92 . . 140 240 35 SER CA C 58.46 . . 141 240 35 SER CB C 63.95 . . 142 240 35 SER N N 117.11 . . 143 241 36 VAL H H 8.13 . . 144 241 36 VAL C C 175.18 . . 145 241 36 VAL CA C 62.34 . . 146 241 36 VAL CB C 32.73 . . 147 241 36 VAL N N 122.59 . . 148 242 37 SER H H 8.37 . . 149 242 37 SER C C 177.09 . . 150 242 37 SER CA C 58.41 . . 151 242 37 SER CB C 63.95 . . 152 242 37 SER N N 120.32 . . 153 243 38 ASP H H 8.37 . . 154 243 38 ASP C C 175.25 . . 155 243 38 ASP CA C 54.41 . . 156 243 38 ASP CB C 41.21 . . 157 243 38 ASP N N 123.83 . . 158 244 39 GLN H H 8.24 . . 159 244 39 GLN C C 175.83 . . 160 244 39 GLN CA C 56.01 . . 161 244 39 GLN CB C 29.17 . . 162 244 39 GLN N N 121.22 . . 163 245 40 PHE H H 8.20 . . 164 245 40 PHE C C 175.86 . . 165 245 40 PHE CA C 57.61 . . 166 245 40 PHE CB C 39.42 . . 167 245 40 PHE N N 121.83 . . 168 246 41 SER H H 8.15 . . 169 246 41 SER C C 177.28 . . 170 246 41 SER CA C 58.12 . . 171 246 41 SER CB C 63.95 . . 172 246 41 SER N N 118.69 . . 173 247 42 VAL H H 8.14 . . 174 247 42 VAL C C 175.57 . . 175 247 42 VAL CA C 62.17 . . 176 247 42 VAL CB C 32.73 . . 177 247 42 VAL N N 122.76 . . 178 248 43 GLU H H 8.37 . . 179 248 43 GLU C C 175.61 . . 180 248 43 GLU CA C 56.47 . . 181 248 43 GLU CB C 30.50 . . 182 248 43 GLU N N 125.21 . . 183 249 44 PHE H H 8.14 . . 184 249 44 PHE C C 176.20 . . 185 249 44 PHE CA C 57.44 . . 186 249 44 PHE CB C 39.87 . . 187 249 44 PHE N N 122.14 . . 188 250 45 GLU H H 8.32 . . 189 250 45 GLU C C 175.57 . . 190 250 45 GLU CA C 56.18 . . 191 250 45 GLU CB C 30.50 . . 192 250 45 GLU N N 124.21 . . 193 251 46 VAL H H 8.24 . . 194 251 46 VAL C C 175.37 . . 195 251 46 VAL CA C 62.28 . . 196 251 46 VAL CB C 33.18 . . 197 251 46 VAL N N 123.06 . . 198 252 47 GLU H H 8.56 . . 199 252 47 GLU C C 175.22 . . 200 252 47 GLU CA C 56.52 . . 201 252 47 GLU CB C 30.51 . . 202 252 47 GLU N N 126.26 . . 203 253 48 SER H H 8.43 . . 204 253 48 SER C C 177.08 . . 205 253 48 SER CA C 58.01 . . 206 253 48 SER CB C 63.95 . . 207 253 48 SER N N 118.83 . . 208 254 49 LEU H H 8.40 . . 209 254 49 LEU C C 174.34 . . 210 254 49 LEU CA C 55.10 . . 211 254 49 LEU CB C 42.55 . . 212 254 49 LEU N N 126.15 . . 213 255 50 ASP H H 8.40 . . 214 255 50 ASP C C 175.29 . . 215 255 50 ASP CA C 54.36 . . 216 255 50 ASP CB C 41.21 . . 217 255 50 ASP N N 122.62 . . 218 256 51 SER H H 8.25 . . 219 256 51 SER C C 176.87 . . 220 256 51 SER CA C 58.40 . . 221 256 51 SER CB C 63.95 . . 222 256 51 SER N N 117.43 . . 223 257 52 GLU H H 8.43 . . 224 257 52 GLU C C 175.72 . . 225 257 52 GLU CA C 56.47 . . 226 257 52 GLU CB C 30.06 . . 227 257 52 GLU N N 123.96 . . 228 258 53 ASP H H 8.27 . . 229 258 53 ASP C C 175.45 . . 230 258 53 ASP CA C 54.07 . . 231 258 53 ASP CB C 41.21 . . 232 258 53 ASP N N 122.30 . . 233 259 54 TYR H H 8.19 . . 234 259 54 TYR C C 175.45 . . 235 259 54 TYR CA C 58.00 . . 236 259 54 TYR CB C 38.53 . . 237 259 54 TYR N N 123.28 . . 238 260 55 SER H H 8.30 . . 239 260 55 SER C C 177.09 . . 240 260 55 SER CA C 58.75 . . 241 260 55 SER CB C 63.95 . . 242 260 55 SER N N 118.85 . . 243 261 56 LEU H H 8.14 . . 244 261 56 LEU C C 174.03 . . 245 261 56 LEU CA C 55.21 . . 246 261 56 LEU CB C 42.55 . . 247 261 56 LEU N N 125.11 . . 248 262 57 SER H H 8.26 . . 249 262 57 SER C C 176.89 . . 250 262 57 SER CA C 58.34 . . 251 262 57 SER CB C 63.95 . . 252 262 57 SER N N 117.84 . . 253 263 58 GLU H H 8.49 . . 254 263 58 GLU C C 174.93 . . 255 263 58 GLU CA C 56.58 . . 256 263 58 GLU CB C 30.06 . . 257 263 58 GLU N N 124.15 . . 258 264 59 GLU H H 8.45 . . 259 264 59 GLU C C 174.40 . . 260 264 59 GLU CA C 56.86 . . 261 264 59 GLU CB C 30.06 . . 262 264 59 GLU N N 123.07 . . 263 265 60 GLY H H 8.52 . . 264 265 60 GLY C C 177.35 . . 265 265 60 GLY CA C 45.40 . . 266 265 60 GLY N N 111.42 . . 267 266 61 GLN H H 8.21 . . 268 266 61 GLN C C 175.65 . . 269 266 61 GLN CA C 55.66 . . 270 266 61 GLN CB C 30.06 . . 271 266 61 GLN N N 120.92 . . 272 267 62 GLU H H 8.60 . . 273 267 62 GLU C C 175.25 . . 274 267 62 GLU CA C 56.46 . . 275 267 62 GLU CB C 30.06 . . 276 267 62 GLU N N 123.81 . . 277 268 63 LEU H H 8.40 . . 278 268 63 LEU C C 174.22 . . 279 268 63 LEU CA C 54.93 . . 280 268 63 LEU CB C 42.55 . . 281 268 63 LEU N N 125.33 . . 282 269 64 SER H H 8.44 . . 283 269 64 SER C C 177.22 . . 284 269 64 SER CA C 57.95 . . 285 269 64 SER CB C 64.39 . . 286 269 64 SER N N 118.38 . . 287 270 65 ASP H H 8.49 . . 288 270 65 ASP C C 175.23 . . 289 270 65 ASP CA C 54.30 . . 290 270 65 ASP CB C 41.21 . . 291 270 65 ASP N N 124.30 . . 292 271 66 GLU H H 8.38 . . 293 271 66 GLU C C 175.28 . . 294 271 66 GLU CA C 56.64 . . 295 271 66 GLU CB C 30.50 . . 296 271 66 GLU N N 122.09 . . 297 272 67 ASP H H 8.40 . . 298 272 67 ASP C C 175.51 . . 299 272 67 ASP CA C 54.36 . . 300 272 67 ASP CB C 41.21 . . 301 272 67 ASP N N 122.70 . . 302 273 68 ASP H H 8.31 . . 303 273 68 ASP C C 175.09 . . 304 273 68 ASP CA C 54.47 . . 305 273 68 ASP CB C 41.21 . . 306 273 68 ASP N N 122.43 . . 307 274 69 GLU H H 8.35 . . 308 274 69 GLU C C 175.01 . . 309 274 69 GLU CA C 56.87 . . 310 274 69 GLU CB C 30.06 . . 311 274 69 GLU N N 122.51 . . 312 275 70 VAL H H 8.08 . . 313 275 70 VAL C C 175.47 . . 314 275 70 VAL CA C 62.68 . . 315 275 70 VAL CB C 32.74 . . 316 275 70 VAL N N 122.12 . . 317 276 71 TYR H H 8.21 . . 318 276 71 TYR C C 175.93 . . 319 276 71 TYR CA C 57.95 . . 320 276 71 TYR CB C 38.98 . . 321 276 71 TYR N N 125.21 . . 322 277 72 GLN H H 8.19 . . 323 277 72 GLN C C 176.11 . . 324 277 72 GLN CA C 55.44 . . 325 277 72 GLN CB C 29.61 . . 326 277 72 GLN N N 123.85 . . 327 278 73 VAL H H 8.17 . . 328 278 73 VAL C C 175.25 . . 329 278 73 VAL CA C 62.39 . . 330 278 73 VAL CB C 32.74 . . 331 278 73 VAL N N 123.16 . . 332 279 74 THR H H 8.30 . . 333 279 74 THR C C 177.44 . . 334 279 74 THR CA C 62.11 . . 335 279 74 THR CB C 69.74 . . 336 279 74 THR N N 120.85 . . 337 280 75 VAL H H 8.19 . . 338 280 75 VAL C C 176.00 . . 339 280 75 VAL CA C 62.11 . . 340 280 75 VAL CB C 32.74 . . 341 280 75 VAL N N 124.83 . . 342 281 76 TYR H H 8.35 . . 343 281 76 TYR C C 176.08 . . 344 281 76 TYR CA C 58.01 . . 345 281 76 TYR CB C 38.98 . . 346 281 76 TYR N N 126.22 . . 347 282 77 GLN H H 8.28 . . 348 282 77 GLN C C 176.57 . . 349 282 77 GLN CA C 55.15 . . 350 282 77 GLN CB C 30.06 . . 351 282 77 GLN N N 125.04 . . 352 283 78 ALA H H 8.35 . . 353 283 78 ALA C C 173.34 . . 354 283 78 ALA CA C 52.70 . . 355 283 78 ALA CB C 18.92 . . 356 283 78 ALA N N 127.46 . . 357 284 79 GLY H H 8.43 . . 358 284 79 GLY C C 177.30 . . 359 284 79 GLY CA C 45.17 . . 360 284 79 GLY N N 109.80 . . 361 285 80 GLU H H 8.33 . . 362 285 80 GLU C C 174.89 . . 363 285 80 GLU CA C 56.58 . . 364 285 80 GLU CB C 30.51 . . 365 285 80 GLU N N 121.78 . . 366 286 81 SER H H 8.44 . . 367 286 81 SER C C 177.27 . . 368 286 81 SER CA C 58.17 . . 369 286 81 SER CB C 64.31 . . 370 286 81 SER N N 117.29 . . 371 287 82 ASP H H 8.47 . . 372 287 82 ASP C C 175.02 . . 373 287 82 ASP CA C 54.41 . . 374 287 82 ASP CB C 41.21 . . 375 287 82 ASP N N 123.87 . . 376 288 83 THR H H 8.14 . . 377 288 83 THR C C 177.06 . . 378 288 83 THR CA C 61.88 . . 379 288 83 THR CB C 69.74 . . 380 288 83 THR N N 114.90 . . 381 289 84 ASP H H 8.36 . . 382 289 84 ASP C C 175.43 . . 383 289 84 ASP CA C 54.53 . . 384 289 84 ASP CB C 41.21 . . 385 289 84 ASP N N 124.09 . . 386 290 85 SER H H 8.17 . . 387 290 85 SER C C 177.47 . . 388 290 85 SER CA C 58.17 . . 389 290 85 SER CB C 63.95 . . 390 290 85 SER N N 116.97 . . 391 291 86 PHE H H 8.26 . . 392 291 86 PHE C C 176.11 . . 393 291 86 PHE CA C 57.61 . . 394 291 86 PHE CB C 39.87 . . 395 291 86 PHE N N 123.55 . . 396 292 87 GLU H H 8.25 . . 397 292 87 GLU C C 175.80 . . 398 292 87 GLU CA C 56.07 . . 399 292 87 GLU CB C 30.50 . . 400 292 87 GLU N N 124.09 . . 401 293 88 GLU H H 8.35 . . 402 293 88 GLU C C 175.75 . . 403 293 88 GLU CA C 56.12 . . 404 293 88 GLU CB C 30.50 . . 405 293 88 GLU N N 123.54 . . 406 294 89 ASP H H 8.54 . . 407 294 89 ASP C C 176.77 . . 408 294 89 ASP CA C 52.25 . . 409 294 89 ASP CB C 41.21 . . 410 294 89 ASP N N 124.70 . . 411 295 90 PRO C C 174.21 . . 412 295 90 PRO CA C 63.48 . . 413 295 90 PRO CB C 32.29 . . 414 296 91 GLU H H 8.51 . . 415 296 91 GLU C C 174.59 . . 416 296 91 GLU CA C 56.92 . . 417 296 91 GLU CB C 30.06 . . 418 296 91 GLU N N 121.07 . . 419 297 92 ILE H H 7.94 . . 420 297 92 ILE C C 175.19 . . 421 297 92 ILE CA C 61.37 . . 422 297 92 ILE CB C 38.97 . . 423 297 92 ILE N N 122.21 . . 424 298 93 SER H H 8.33 . . 425 298 93 SER C C 176.71 . . 426 298 93 SER CA C 58.23 . . 427 298 93 SER CB C 63.50 . . 428 298 93 SER N N 120.35 . . 429 299 94 LEU H H 8.25 . . 430 299 94 LEU C C 174.16 . . 431 299 94 LEU CA C 55.44 . . 432 299 94 LEU CB C 42.10 . . 433 299 94 LEU N N 125.88 . . 434 300 95 ALA H H 8.10 . . 435 300 95 ALA C C 173.77 . . 436 300 95 ALA CA C 52.98 . . 437 300 95 ALA CB C 18.92 . . 438 300 95 ALA N N 124.69 . . 439 301 96 ASP H H 8.11 . . 440 301 96 ASP C C 175.42 . . 441 301 96 ASP CA C 54.47 . . 442 301 96 ASP CB C 40.76 . . 443 301 96 ASP N N 119.45 . . 444 302 97 TYR H H 7.72 . . 445 302 97 TYR C C 176.07 . . 446 302 97 TYR CA C 58.41 . . 447 302 97 TYR CB C 38.53 . . 448 302 97 TYR N N 120.70 . . 449 303 98 TRP H H 7.76 . . 450 303 98 TRP C C 176.57 . . 451 303 98 TRP CA C 56.81 . . 452 303 98 TRP CB C 29.62 . . 453 303 98 TRP N N 122.56 . . 454 304 99 LYS H H 7.45 . . 455 304 99 LYS C C 170.55 . . 456 304 99 LYS CA C 57.83 . . 457 304 99 LYS CB C 33.63 . . 458 304 99 LYS N N 128.55 . . stop_ save_