data_1497 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; ADR1a, a Zinc Finger Peptide, Exists in Two Folded Conformations ; _BMRB_accession_number 1497 _BMRB_flat_file_name bmr1497.str _Entry_type update _Submission_date 1995-07-31 _Accession_date 1996-03-25 _Entry_origination BMRB _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Xu Robert X. . 2 Horvath Suzanne . . 3 Klevit Rachel E. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 190 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-14 revision BMRB 'Complete natural source information' 1999-06-14 revision BMRB 'Converted to BMRB NMR-STAR V 2.1 format' 1996-03-25 reformat BMRB 'Converted to the BMRB 1996-03-01 STAR flat-file format' 1995-07-31 original BMRB 'Last release in original BMRB flat-file format' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full ; Xu, Robert X., Horvath, Suzanne, Klevit, Rachel E., "ADR1a, a Zinc Finger Peptide, Exists in Two Folded Conformations," Biochemistry 30, 3365-3371 (1991). ; _Citation_title 'ADR1a, a Zinc Finger Peptide, Exists in Two Folded Conformations' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Xu Robert X. . 2 Horvath Suzanne . . 3 Klevit Rachel E. . stop_ _Journal_abbreviation Biochemistry _Journal_volume 30 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 3365 _Page_last 3371 _Year 1991 _Details . save_ ################################## # Molecular system description # ################################## save_system_transcriptional_activator_ADR1 _Saveframe_category molecular_system _Mol_system_name 'transcriptional activator ADR1' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'transcriptional activator ADR1' $transcriptional_activator_ADR1 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic ? _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_transcriptional_activator_ADR1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'transcriptional activator ADR1' _Name_variant 'residues 130-159, zinc finger domain ADR1a' _Molecular_mass . _Mol_thiol_state . _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 30 _Mol_residue_sequence ; KPYPCGLCNRCFTRRDLLIR HAQKIHSGNL ; loop_ _Residue_seq_code _Residue_label 1 LYS 2 PRO 3 TYR 4 PRO 5 CYS 6 GLY 7 LEU 8 CYS 9 ASN 10 ARG 11 CYS 12 PHE 13 THR 14 ARG 15 ARG 16 ASP 17 LEU 18 LEU 19 ILE 20 ARG 21 HIS 22 ALA 23 GLN 24 LYS 25 ILE 26 HIS 27 SER 28 GLY 29 ASN 30 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-09-23 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 1496 "transcriptional activator ADR1" 100.00 30 100.00 100.00 1.29e-12 PDB 5A7U "Single-particle Cryo-em Of Co-translational Folded Adr1 Domain Inside The E. Coli Ribosome Exit Tunnel" 93.33 29 100.00 100.00 2.60e-11 DBJ GAA22443 "K7_Adr1ap [Saccharomyces cerevisiae Kyokai no. 7]" 100.00 219 100.00 100.00 5.61e-13 EMBL CAA92359 "Adr1p [Saccharomyces cerevisiae]" 100.00 973 100.00 100.00 2.14e-13 EMBL CAA92367 "Adr1p [Saccharomyces cerevisiae]" 100.00 973 100.00 100.00 2.14e-13 GB AAA73863 "Adr1p [Saccharomyces cerevisiae]" 100.00 1323 100.00 100.00 1.66e-13 GB AHY75198 "Adr1p [Saccharomyces cerevisiae YJM993]" 100.00 1323 100.00 100.00 1.53e-13 GB AJP37926 "Adr1p [Saccharomyces cerevisiae YJM1078]" 100.00 1323 100.00 100.00 1.53e-13 GB AJU58048 "Adr1p [Saccharomyces cerevisiae YJM189]" 100.00 1323 100.00 100.00 1.53e-13 GB AJU58743 "Adr1p [Saccharomyces cerevisiae YJM193]" 100.00 1323 100.00 100.00 1.49e-13 PRF 1204267A "ADR1 gene" 100.00 1323 100.00 100.00 1.53e-13 REF NP_010502 "DNA-binding transcription factor ADR1 [Saccharomyces cerevisiae S288c]" 100.00 1323 100.00 100.00 1.53e-13 SP P07248 "RecName: Full=Regulatory protein ADR1" 100.00 1323 100.00 100.00 1.53e-13 TPG DAA12059 "TPA: DNA-binding transcription factor ADR1 [Saccharomyces cerevisiae S288c]" 100.00 1323 100.00 100.00 1.53e-13 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $transcriptional_activator_ADR1 yeast 4932 Eukaryota Fungi Saccharomyces cerevisiae stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $transcriptional_activator_ADR1 'not available' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_list _Saveframe_category NMR_spectrometer _Manufacturer unknown _Model unknown _Field_strength 0 _Details 'spectrometer information not available' save_ ############################# # NMR applied experiments # ############################# save__1 _Saveframe_category NMR_applied_experiment _Sample_label $sample_one save_ ####################### # Sample conditions # ####################### save_sample_condition_set_one _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.45 . na temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_par_set_one _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio_citation_label _Correction_value_citation_label H2O/HDO H . . ppm 4.8 . . . . . $entry_citation $entry_citation stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_chemical_shift_assignment_data_set_one _Saveframe_category assigned_chemical_shifts _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_condition_set_one _Chem_shift_reference_set_label $chem_shift_reference_par_set_one _Mol_system_component_name 'transcriptional activator ADR1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 . 1 LYS HA H 4.31 . 1 2 . 1 LYS HB2 H 1.9 . 1 3 . 1 LYS HB3 H 1.9 . 1 4 . 1 LYS HG2 H 1.46 . 1 5 . 1 LYS HG3 H 1.46 . 1 6 . 1 LYS HD2 H 1.68 . 1 7 . 1 LYS HD3 H 1.68 . 1 8 . 1 LYS HE2 H 2.98 . 1 9 . 1 LYS HE3 H 2.98 . 1 10 . 2 PRO HA H 4.47 . 1 11 . 2 PRO HB2 H 2.26 . 1 12 . 2 PRO HB3 H 2.26 . 1 13 . 2 PRO HG2 H 1.98 . 1 14 . 2 PRO HG3 H 1.98 . 1 15 . 2 PRO HD2 H 3.56 . 2 16 . 2 PRO HD3 H 3.68 . 2 17 . 3 TYR H H 8.72 . 1 18 . 3 TYR HA H 5.23 . 1 19 . 3 TYR HB2 H 2.76 . 2 20 . 3 TYR HB3 H 2.94 . 2 21 . 3 TYR HD1 H 7.04 . 1 22 . 3 TYR HD2 H 7.04 . 1 23 . 3 TYR HE1 H 6.85 . 1 24 . 3 TYR HE2 H 6.85 . 1 25 . 4 PRO HA H 4.62 . 1 26 . 4 PRO HG2 H 1.98 . 2 27 . 4 PRO HG3 H 2.1 . 2 28 . 4 PRO HD2 H 3.4 . 2 29 . 4 PRO HD3 H 3.96 . 2 30 . 5 CYS H H 8.72 . 1 31 . 5 CYS HA H 4.48 . 1 32 . 5 CYS HB2 H 2.76 . 2 33 . 5 CYS HB3 H 3.39 . 2 34 . 6 GLY H H 9.18 . 1 35 . 6 GLY HA2 H 3.89 . 2 36 . 6 GLY HA3 H 4.21 . 2 37 . 7 LEU H H 9.2 . 1 38 . 7 LEU HA H 4.47 . 1 39 . 7 LEU HB2 H .45 . 2 40 . 7 LEU HB3 H 1.01 . 2 41 . 7 LEU HG H 1.32 . 1 42 . 7 LEU HD1 H .55 . 2 43 . 7 LEU HD2 H .72 . 2 44 . 8 CYS H H 7.95 . 1 45 . 8 CYS HA H 4.96 . 1 46 . 8 CYS HB2 H 3.3 . 2 47 . 8 CYS HB3 H 3.33 . 2 48 . 9 ASN H H 8.16 . 1 49 . 9 ASN HA H 4.84 . 1 50 . 9 ASN HB2 H 2.89 . 2 51 . 9 ASN HB3 H 2.98 . 2 52 . 9 ASN HD21 H 6.85 . 2 53 . 9 ASN HD22 H 7.55 . 2 54 . 10 ARG H H 8.4 . 1 55 . 10 ARG HA H 4.1 . 1 56 . 10 ARG HB2 H 1.24 . 2 57 . 10 ARG HB3 H 1.31 . 2 58 . 10 ARG HG2 H 1.7 . 2 59 . 10 ARG HG3 H 1.76 . 2 60 . 10 ARG HD2 H 3.12 . 1 61 . 10 ARG HD3 H 3.12 . 1 62 . 10 ARG HE H 7.18 . 1 63 . 11 CYS H H 7.96 . 1 64 . 11 CYS HA H 4.59 . 1 65 . 11 CYS HB2 H 2.68 . 2 66 . 11 CYS HB3 H 2.89 . 2 67 . 12 PHE H H 8.24 . 1 68 . 12 PHE HA H 4.91 . 1 69 . 12 PHE HB2 H 3.02 . 2 70 . 12 PHE HB3 H 3.26 . 2 71 . 12 PHE HD1 H 7.33 . 1 72 . 12 PHE HD2 H 7.33 . 1 73 . 12 PHE HE1 H 6.81 . 1 74 . 12 PHE HE2 H 6.81 . 1 75 . 12 PHE HZ H 6.13 . 1 76 . 13 THR H H 8.91 . 1 77 . 13 THR HA H 4.78 . 1 78 . 13 THR HB H 4.53 . 1 79 . 13 THR HG2 H 1.42 . 1 80 . 14 ARG H H 7.12 . 1 81 . 14 ARG HA H 4.7 . 1 82 . 14 ARG HB2 H 1.59 . 2 83 . 14 ARG HB3 H 2.14 . 2 84 . 14 ARG HG2 H 1.74 . 1 85 . 14 ARG HG3 H 1.74 . 1 86 . 14 ARG HD2 H 3.3 . 1 87 . 14 ARG HD3 H 3.3 . 1 88 . 14 ARG HE H 8.52 . 1 89 . 15 ARG H H 8.51 . 1 90 . 15 ARG HB2 H 1.34 . 1 91 . 15 ARG HB3 H 1.34 . 1 92 . 15 ARG HG2 H 1.22 . 2 93 . 15 ARG HG3 H 1.52 . 2 94 . 15 ARG HD2 H 3.05 . 1 95 . 15 ARG HD3 H 3.05 . 1 96 . 15 ARG HE H 7.34 . 1 97 . 16 ASP H H 8.66 . 1 98 . 16 ASP HA H 4.12 . 1 99 . 16 ASP HB2 H 2.56 . 2 100 . 16 ASP HB3 H 2.67 . 2 101 . 17 LEU H H 6.88 . 1 102 . 17 LEU HA H 3.97 . 1 103 . 17 LEU HB2 H 1.52 . 2 104 . 17 LEU HB3 H 1.94 . 2 105 . 17 LEU HG H 1.65 . 1 106 . 17 LEU HD1 H .89 . 2 107 . 17 LEU HD2 H 1.14 . 2 108 . 18 LEU H H 6.96 . 1 109 . 18 LEU HA H 3.33 . 1 110 . 18 LEU HB2 H 1.48 . 2 111 . 18 LEU HB3 H 2.1 . 2 112 . 18 LEU HG H 1.57 . 1 113 . 18 LEU HD1 H 1.04 . 2 114 . 18 LEU HD2 H 1.14 . 2 115 . 19 ILE H H 8.12 . 1 116 . 19 ILE HA H 3.61 . 1 117 . 19 ILE HB H 1.8 . 1 118 . 19 ILE HG12 H 1.53 . 2 119 . 19 ILE HG13 H 1.23 . 2 120 . 19 ILE HG2 H .85 . 1 121 . 19 ILE HD1 H .75 . 1 122 . 20 ARG H H 7.68 . 1 123 . 20 ARG HA H 4.02 . 1 124 . 20 ARG HB2 H 1.82 . 1 125 . 20 ARG HB3 H 1.82 . 1 126 . 20 ARG HG2 H 1.66 . 2 127 . 20 ARG HG3 H 1.74 . 2 128 . 20 ARG HD2 H 3.17 . 1 129 . 20 ARG HD3 H 3.17 . 1 130 . 20 ARG HE H 7.28 . 1 131 . 21 HIS H H 7.52 . 1 132 . 21 HIS HA H 4.27 . 1 133 . 21 HIS HB2 H 2.78 . 2 134 . 21 HIS HB3 H 3.21 . 2 135 . 21 HIS HD2 H 7.16 . 1 136 . 21 HIS HE1 H 7.97 . 1 137 . 22 ALA H H 8.92 . 1 138 . 22 ALA HA H 3.8 . 1 139 . 22 ALA HB H 1.59 . 1 140 . 23 GLN H H 8.2 . 1 141 . 23 GLN HA H 3.81 . 1 142 . 23 GLN HB2 H 2.03 . 2 143 . 23 GLN HB3 H 2.21 . 2 144 . 23 GLN HG2 H 2.36 . 2 145 . 23 GLN HG3 H 2.56 . 2 146 . 23 GLN HE21 H 6.75 . 2 147 . 23 GLN HE22 H 7.39 . 2 148 . 24 LYS H H 7.76 . 1 149 . 24 LYS HA H 4.14 . 1 150 . 24 LYS HB2 H 1.88 . 2 151 . 24 LYS HB3 H 1.82 . 2 152 . 24 LYS HG2 H 1.42 . 2 153 . 24 LYS HG3 H 1.48 . 2 154 . 24 LYS HD2 H 1.67 . 1 155 . 24 LYS HD3 H 1.67 . 1 156 . 24 LYS HE2 H 2.97 . 1 157 . 24 LYS HE3 H 2.97 . 1 158 . 25 ILE H H 8.24 . 1 159 . 25 ILE HA H 4.12 . 1 160 . 25 ILE HB H .92 . 1 161 . 25 ILE HG12 H 1.31 . 2 162 . 25 ILE HG13 H .98 . 2 163 . 25 ILE HG2 H .3 . 1 164 . 25 ILE HD1 H .68 . 1 165 . 26 HIS H H 7.32 . 1 166 . 26 HIS HA H 5.26 . 1 167 . 26 HIS HB2 H 3.22 . 2 168 . 26 HIS HB3 H 3.4 . 2 169 . 26 HIS HD2 H 6.5 . 1 170 . 26 HIS HE1 H 7.91 . 1 171 . 27 SER H H 8.14 . 1 172 . 27 SER HA H 4.26 . 1 173 . 27 SER HB2 H 3.97 . 2 174 . 27 SER HB3 H 4.04 . 2 175 . 28 GLY H H 8.48 . 1 176 . 28 GLY HA2 H 4.07 . 2 177 . 28 GLY HA3 H 3.54 . 2 178 . 29 ASN H H 8.01 . 1 179 . 29 ASN HA H 4.65 . 1 180 . 29 ASN HB2 H 2.72 . 2 181 . 29 ASN HB3 H 2.81 . 2 182 . 29 ASN HD21 H 6.97 . 2 183 . 29 ASN HD22 H 7.47 . 2 184 . 30 LEU H H 7.9 . 1 185 . 30 LEU HA H 4.2 . 1 186 . 30 LEU HB2 H 1.59 . 1 187 . 30 LEU HB3 H 1.59 . 1 188 . 30 LEU HG H 1.59 . 1 189 . 30 LEU HD1 H .84 . 2 190 . 30 LEU HD2 H .9 . 2 stop_ save_