data_4970 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; NMR Relaxation data for Protein Calmodulin in complex with the smooth muscle myosin light chain kinase calmodulin binding domain ; _BMRB_accession_number 4970 _BMRB_flat_file_name bmr4970.str _Entry_type update _Submission_date 2001-03-12 _Accession_date 2001-03-12 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lee Andrew L. . 2 Wand A. J. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count T1_relaxation 5 T2_relaxation 39 heteronucl_NOE 5 S2_parameters 18 stop_ loop_ _Data_type _Data_type_count "T1 relaxation values" 442 "T2 relaxation values" 2320 "order parameters" 1143 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2011-09-06 update author 'correction of residue 60' 2011-08-10 update BMRB ; Calmodulin sequence corrected, sample conditions added to represent temperatures used for data collection, atom type information added to order parameter save frames ; 2007-03-14 update author 'correction of natural source' 2005-09-02 update author 'correction of field strength' 2003-06-09 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_primary_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; NMR Relaxation data for Protein Calmodulin in complex with the smooth muscle myosin light chain kinase calmodulin binding domain ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 11373686 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Lee Andrew L. . 2 Wand A. J. . stop_ _Journal_abbreviation Nature _Journal_volume 411 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 501 _Page_last 504 _Year 2001 _Details . loop_ _Keyword 'protein NMR' '15N relaxation' 'correlation time' 'chemical shift anisotropy' 'spin-spin relaxation' 'nuclear Overhauser effect' 'spin-lattice relaxation' stop_ save_ ################################## # Molecular system description # ################################## save_system_Calmodulin _Saveframe_category molecular_system _Mol_system_name Calmodulin _Abbreviation_common Calmodulin _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label Calmodulin $Calmodulin 'smooth muscle myosin light chain kinase' $SMMLCK stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state monomer _System_paramagnetic no _System_thiol_state 'not present' _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Calmodulin _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Calmodulin _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 148 _Mol_residue_sequence ; ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGD GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK ; loop_ _Residue_seq_code _Residue_label 1 ALA 2 ASP 3 GLN 4 LEU 5 THR 6 GLU 7 GLU 8 GLN 9 ILE 10 ALA 11 GLU 12 PHE 13 LYS 14 GLU 15 ALA 16 PHE 17 SER 18 LEU 19 PHE 20 ASP 21 LYS 22 ASP 23 GLY 24 ASP 25 GLY 26 THR 27 ILE 28 THR 29 THR 30 LYS 31 GLU 32 LEU 33 GLY 34 THR 35 VAL 36 MET 37 ARG 38 SER 39 LEU 40 GLY 41 GLN 42 ASN 43 PRO 44 THR 45 GLU 46 ALA 47 GLU 48 LEU 49 GLN 50 ASP 51 MET 52 ILE 53 ASN 54 GLU 55 VAL 56 ASP 57 ALA 58 ASP 59 GLY 60 ASP 61 GLY 62 THR 63 ILE 64 ASP 65 PHE 66 PRO 67 GLU 68 PHE 69 LEU 70 THR 71 MET 72 MET 73 ALA 74 ARG 75 LYS 76 MET 77 LYS 78 ASP 79 THR 80 ASP 81 SER 82 GLU 83 GLU 84 GLU 85 ILE 86 ARG 87 GLU 88 ALA 89 PHE 90 ARG 91 VAL 92 PHE 93 ASP 94 LYS 95 ASP 96 GLY 97 ASN 98 GLY 99 TYR 100 ILE 101 SER 102 ALA 103 ALA 104 GLU 105 LEU 106 ARG 107 HIS 108 VAL 109 MET 110 THR 111 ASN 112 LEU 113 GLY 114 GLU 115 LYS 116 LEU 117 THR 118 ASP 119 GLU 120 GLU 121 VAL 122 ASP 123 GLU 124 MET 125 ILE 126 ARG 127 GLU 128 ALA 129 ASP 130 ILE 131 ASP 132 GLY 133 ASP 134 GLY 135 GLN 136 VAL 137 ASN 138 TYR 139 GLU 140 GLU 141 PHE 142 VAL 143 GLN 144 MET 145 MET 146 THR 147 ALA 148 LYS stop_ _Sequence_homology_query_date 2008-08-19 _Sequence_homology_query_revised_last_date 2008-08-19 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value SWISS-PROT P62149 'Calmodulin (CaM)' 100.00 149 100.00 100.00 3.91e-79 SWISS-PROT P62151 'Calmodulin (CaM)' 100.00 149 100.00 100.00 3.91e-79 SWISS-PROT P21251 'Calmodulin (CaM)' 100.00 149 98.65 99.32 3.66e-78 SWISS-PROT P62144 'Calmodulin (CaM)' 100.00 149 100.00 100.00 3.91e-79 REF NP_001084025 'cam [Xenopus laevis]' 100.00 149 100.00 100.00 3.91e-79 SWISS-PROT P02594 'Calmodulin (CaM)' 100.00 149 99.32 100.00 9.56e-79 REF NP_001039714 'calmodulin 3 [Bos taurus]' 100.00 149 100.00 100.00 3.91e-79 REF NP_001080864 'calmodulin 2 [Xenopus laevis]' 100.00 149 100.00 100.00 3.91e-79 REF NP_001008160 'calmodulin 2 (phosphorylase kinase, delta) [Xenopus tropicalis]' 100.00 149 100.00 100.00 3.91e-79 REF NP_001009759 'calmodulin 2 [Ovis aries]' 100.00 149 100.00 100.00 3.91e-79 PIR JC1305 'calmodulin - Japanese medaka' 100.00 149 100.00 100.00 3.91e-79 PIR MCON 'calmodulin - salmon' 100.00 148 100.00 100.00 3.88e-79 GenBank AAA40862 calmodulin 100.00 149 100.00 100.00 3.91e-79 GenBank AAA40863 calmodulin 100.00 149 100.00 100.00 3.91e-79 GenBank AAA35641 calmodulin 100.00 149 100.00 100.00 3.91e-79 GenBank AAA37365 'calmodulin synthesis' 100.00 149 100.00 100.00 3.91e-79 EMBL CAA32478 'calmodulin III [Rattus norvegicus]' 100.00 149 100.00 100.00 3.91e-79 GenBank AAA35635 calmodulin 100.00 149 100.00 100.00 3.91e-79 EMBL CAA32119 'calmodulin [Rattus norvegicus]' 100.00 149 100.00 100.00 3.91e-79 EMBL CAA32120 'calmodulin [Rattus norvegicus]' 100.00 149 100.00 100.00 3.91e-79 EMBL CAA32050 'calmodulin [Rattus norvegicus]' 100.00 149 100.00 100.00 3.91e-79 EMBL CAA32062 'calmodulin II [Rattus norvegicus]' 100.00 149 100.00 100.00 3.91e-79 DBJ BAB28116 'unnamed protein product [Mus musculus]' 100.00 149 100.00 100.00 3.91e-79 DBJ BAB28319 'unnamed protein product [Mus musculus]' 100.00 149 100.00 100.00 3.91e-79 DBJ BAA11896 'calmodulin [Anas platyrhynchos]' 100.00 149 100.00 100.00 3.91e-79 DBJ BAB23462 'unnamed protein product [Mus musculus]' 100.00 149 100.00 100.00 3.91e-79 PDB 3CLN 'Structure Of Calmodulin Refined At 2.2 Angstroms Resolution' 100.00 148 99.32 100.00 1.14e-78 DBJ BAA08302 'calmodulin [Homo sapiens]' 100.00 149 100.00 100.00 3.91e-79 PDB 3BXK 'Crystal Structure Of The PQ-Type Calcium Channel (Cav2.1) Iq Domain And Ca2+calmodulin Complex' 100.00 148 100.00 100.00 3.88e-79 PDB 3BXL 'Crystal Structure Of The R-Type Calcium Channel (Cav2.3) Iq Domain And Ca2+calmodulin Complex' 100.00 148 100.00 100.00 3.88e-79 PDB 2V01 'Recombinant Vertebrate Calmodulin Complexed With Pb' 100.00 149 100.00 100.00 3.91e-79 PDB 2V02 'Recombinant Vertebrate Calmodulin Complexed With Ba' 100.00 149 100.00 100.00 3.91e-79 PDB 2O60 'Calmodulin Bound To Peptide From Neuronal Nitric Oxide Synthase' 100.00 148 100.00 100.00 3.88e-79 PDB 2R28 'The Complex Structure Of Calmodulin Bound To A Calcineurin Peptide' 100.00 149 100.00 100.00 3.91e-79 PDB 2K0F 'Calmodulin Complexed With Calmodulin-Binding Peptide From Smooth Muscle Myosin Light Chain Kinase' 100.00 148 100.00 100.00 3.88e-79 PDB 2O5G 'Calmodulin-Smooth Muscle Light Chain Kinase Peptide Complex' 100.00 148 100.00 100.00 3.88e-79 PDB 2HQW 'Crystal Structure Of Ca2+CALMODULIN BOUND TO NMDA RECEPTOR Nr1c1 Peptide' 100.00 148 100.00 100.00 3.88e-79 PDB 2K0E 'A Coupled Equilibrium Shift Mechanism In Calmodulin- Mediated Signal Transduction' 100.00 148 100.00 100.00 3.88e-79 PDB 2F3Z 'CalmodulinIQ-Aa Domain Complex' 100.00 148 100.00 100.00 3.88e-79 PDB 2FOT 'Crystal Structure Of The Complex Between Calmodulin And Alphaii-Spectrin' 100.00 148 100.00 100.00 3.88e-79 PDB 2F2P 'Structure Of Calmodulin Bound To A Calcineurin Peptide: A New Way Of Making An Old Binding Mode' 100.00 179 100.00 100.00 2.13e-79 PDB 2F3Y 'CalmodulinIQ DOMAIN COMPLEX' 100.00 148 100.00 100.00 3.88e-79 PDB 2DFS '3-D Structure Of Myosin-V Inhibited State' 100.00 148 100.00 100.00 3.88e-79 PDB 2F2O 'Structure Of Calmodulin Bound To A Calcineurin Peptide: A New Way Of Making An Old Binding Mode' 100.00 179 100.00 100.00 2.13e-79 PDB 2BCX 'Crystal Structure Of Calmodulin In Complex With A Ryanodine Receptor Peptide' 100.00 148 100.00 100.00 3.88e-79 PDB 2BKI 'Myosin Vi Nucleotide-Free (Mdinsert2-Iq) Crystal Structure' 100.00 149 100.00 100.00 3.91e-79 PDB 1XFZ 'Crystal Structure Of Anthrax Edema Factor (Ef) In Complex With Calmodulin In The Presence Of 1 Millimolar Exogenously Added Calcium Chloride' 100.00 149 99.32 100.00 7.57e-79 PDB 1YR5 '1.7-A Structure Of Calmodulin Bound To A Peptide From Dap Kinase' 100.00 148 100.00 100.00 3.88e-79 PDB 1XFW "Crystal Structure Of Anthrax Edema Factor (Ef) In Complex With Calmodulin And 3'5' Cyclic Amp (Camp)" 100.00 149 99.32 100.00 7.57e-79 PDB 1XFY 'Crystal Structure Of Anthrax Edema Factor (Ef) In Complex With Calmodulin' 100.00 149 99.32 100.00 7.57e-79 PDB 1XFU 'Crystal Structure Of Anthrax Edema Factor (Ef) Truncation Mutant, Ef-Delta 64 In Complex With Calmodulin' 100.00 149 99.32 100.00 7.57e-79 PDB 1XFV "Crystal Structure Of Anthrax Edema Factor (Ef) In Complex With Calmodulin And 3' Deoxy-Atp" 100.00 149 99.32 100.00 7.57e-79 PDB 1X02 'Solution Structure Of Stereo Array Isotope Labeled (Sail) Calmodulin' 100.00 148 100.00 100.00 3.88e-79 PDB 1XA5 'Structure Of Calmodulin In Complex With Kar-2, A Bis-Indol Alkaloid' 100.00 148 100.00 100.00 3.88e-79 PDB 1UP5 'Chicken Calmodulin' 100.00 148 99.32 99.32 1.98e-78 PDB 1WRZ 'Calmodulin Complexed With A Peptide From A Human Death- Associated Protein Kinase' 100.00 149 100.00 100.00 3.91e-79 PDB 1SK6 "Crystal Structure Of The Adenylyl Cyclase Domain Of Anthrax Edema Factor (Ef) In Complex With Calmodulin, 3',5' Cyclic Amp (Camp), And Pyrophosphate" 100.00 148 100.00 100.00 3.88e-79 PDB 1SY9 'Structure Of Calmodulin Complexed With A Fragment Of The Olfactory Cng Channel' 100.00 148 100.00 100.00 3.88e-79 PDB 1QX5 'Crystal Structure Of Apocalmodulin' 100.00 148 100.00 100.00 3.88e-79 PDB 1S26 'Structure Of Anthrax Edema Factor-Calmodulin-Alpha,Beta- Methyleneadenosine 5'-Triphosphate Complex Reveals An Alternative Mode Of Atp Binding To The Catalytic Site' 100.00 148 100.00 100.00 3.88e-79 PDB 1QIV 'Calmodulin Complexed With N-(3,3,-Diphenylpropyl)-N'-[1-R- (3,4-Bis-Butoxyphenyl)-Ethyl]-Propylenediamine (Dpd), 1:2 Complex' 100.00 148 100.00 100.00 3.88e-79 PDB 1QIW 'Calmodulin Complexed With N-(3,3,-Diphenylpropyl)-N'-[1-R- (3,4-Bis-Butoxyphenyl)-Ethyl]-Propylenediamine (Dpd)' 100.00 148 100.00 100.00 3.88e-79 PDB 1NWD 'Solution Structure Of Ca2+CALMODULIN BOUND TO THE C- Terminal Domain Of Petunia Glutamate Decarboxylase' 100.00 148 100.00 100.00 3.88e-79 PDB 1PRW 'Crystal Structure Of Bovine Brain Ca++ Calmodulin In A Compact Form' 100.00 148 99.32 99.32 1.98e-78 PDB 1LVC "Crystal Structure Of The Adenylyl Cyclase Domain Of Anthrax Edema Factor (Ef) In Complex With Calmodulin And 2' Deoxy, 3' Anthraniloyl Atp" 100.00 149 100.00 100.00 3.91e-79 PDB 1MUX 'Solution Nmr Structure Of CalmodulinW-7 Complex: The Basis Of Diversity In Molecular Recognition, 30 Structures' 100.00 148 100.00 100.00 3.88e-79 PDB 1L7Z 'Crystal Structure Of Ca2+CALMODULIN COMPLEXED WITH Myristoylated Cap-23NAP-22 Peptide' 100.00 148 100.00 100.00 3.88e-79 PDB 1LIN 'Calmodulin Complexed With Trifluoperazine (1:4 Complex)' 100.00 148 100.00 100.00 3.88e-79 PDB 1IWQ 'Crystal Structure Of Marcks Calmodulin Binding Domain Peptide Complexed With Ca2+CALMODULIN' 100.00 148 100.00 100.00 3.88e-79 PDB 1K90 "Crystal Structure Of The Adenylyl Cyclase Domain Of Anthrax Edema Factor (Ef) In Complex With Calmodulin And 3' Deoxy- Atp" 100.00 148 100.00 100.00 3.88e-79 PDB 1G4Y '1.60 A Crystal Structure Of The Gating Domain From Small Conductance Potassium Channel Complexed With Calcium- Calmodulin' 100.00 148 100.00 100.00 3.88e-79 PDB 1IQ5 'CalmodulinNEMATODE CA2+CALMODULIN DEPENDENT KINASE KINASE Fragment' 100.00 149 100.00 100.00 3.91e-79 PDB 1CTR 'Drug Binding By Calmodulin: Crystal Structure Of A Calmodulin-Trifluoperazine Complex' 100.00 148 100.00 100.00 3.88e-79 PDB 1DMO 'Calmodulin, Nmr, 30 Structures' 100.00 148 99.32 100.00 1.14e-78 PDB 1CM1 'Motions Of Calmodulin-Single-Conformer Refinement' 100.00 148 100.00 100.00 3.88e-79 PDB 1CM4 'Motions Of Calmodulin-Four-Conformer Refinement' 100.00 148 100.00 100.00 3.88e-79 PDB 1CKK 'CalmodulinRAT CA2+CALMODULIN DEPENDENT PROTEIN KINASE Fragment' 100.00 148 100.00 100.00 3.88e-79 PDB 1CLL 'Calmodulin Structure Refined At 1.7 Angstroms Resolution' 100.00 148 100.00 100.00 3.88e-79 PDB 1CFD 'Calcium-Free Calmodulin' 100.00 148 100.00 100.00 3.88e-79 PDB 1CFF 'Nmr Solution Structure Of A Complex Of Calmodulin With A Binding Peptide Of The Ca2+-Pump' 100.00 148 100.00 100.00 3.88e-79 PDB 1A29 'Calmodulin Complexed With Trifluoperazine (1:2 Complex)' 100.00 148 100.00 100.00 3.88e-79 PDB 1CFC 'Calcium-Free Calmodulin' 100.00 148 100.00 100.00 3.88e-79 BMRB 6023 Calmodulin 100.00 148 100.00 100.00 3.88e-79 BMRB 6541 calmodulin 100.00 148 100.00 100.00 3.88e-79 BMRB 5480 Calmodulin 100.00 148 100.00 100.00 3.88e-79 BMRB 5770 Calmodulin 100.00 148 100.00 100.00 3.88e-79 BMRB 5286 calmodulin 100.00 148 100.00 100.00 3.88e-79 BMRB 5287 calmodulin 100.00 148 100.00 100.00 3.88e-79 BMRB 4310 calmodulin 100.00 148 100.00 100.00 3.88e-79 BMRB 5227 'calmodulin (chicken)' 100.00 148 100.00 100.00 3.88e-79 BMRB 4270 calmodulin 100.00 148 100.00 100.00 3.88e-79 BMRB 4284 Calmodulin 100.00 148 100.00 100.00 3.88e-79 BMRB 15191 Calmodulin 100.00 148 98.65 100.00 1.51e-78 BMRB 4056 calmodulin 100.00 148 100.00 100.00 3.88e-79 BMRB 15187 calmodulin 100.00 149 98.65 100.00 1.60e-78 BMRB 15188 calmodulin 100.00 148 98.65 100.00 1.51e-78 BMRB 15185 calmodulin 100.00 148 98.65 100.00 1.51e-78 BMRB 15186 calmodulin 100.00 148 98.65 100.00 1.51e-78 BMRB 15183 calmodulin 100.00 149 98.65 100.00 1.60e-78 BMRB 15184 calmodulin 100.00 148 98.65 100.00 1.51e-78 stop_ save_ save_SMMLCK _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'smooth muscle myosin light chain kinase calmodulin binding domain' _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 21 _Mol_residue_sequence ; GSARRKWQKTGHAVRAIGRL S ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 SER 3 ALA 4 ARG 5 ARG 6 LYS 7 TRP 8 GLN 9 LYS 10 THR 11 GLY 12 HIS 13 ALA 14 VAL 15 ARG 16 ALA 17 ILE 18 GLY 19 ARG 20 LEU 21 SER stop_ _Sequence_homology_query_date 2008-08-19 _Sequence_homology_query_revised_last_date 2008-08-19 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value REF NP_990790 'myosin, light chain kinase [Gallus gallus]' 90.48 1906 100.00 100.00 7.29e-03 SWISS-PROT P11799 'Myosin light chain kinase, smooth muscle (MLCK) (Telokin)' 90.48 1906 100.00 100.00 7.29e-03 GenBank AAC29031 'smooth muscle/non-muscle myosin light chain kinase [Gallus gallus]' 90.48 1906 100.00 100.00 7.29e-03 REF NP_001099152 'myosin, light chain kinase [Danio rerio]' 85.71 899 100.00 100.00 6.65e-03 GenBank AAB41402 'neuronal myosin light chain kinase 1' 85.71 907 100.00 100.00 1.03e-02 GenBank AAB53767 'myosin light chain kinase [Gallus gallus]' 90.48 212 100.00 100.00 7.92e-03 GenBank AAA49069 'smooth muscle myosin light chain kinase precursor (EC 2.7.2.37)' 90.48 972 100.00 100.00 2.82e-03 GenBank AAA69964 'myosin light chain kinase' 90.48 648 100.00 100.00 2.12e-03 EMBL CAA37059 'unnamed protein product [Gallus gallus]' 90.48 603 100.00 100.00 5.31e-03 EMBL CAF90187 'unnamed protein product [Tetraodon nigroviridis]' 85.71 346 100.00 100.00 6.65e-03 EMBL CAA37056 'myosin light chain kinase [Gallus gallus]' 90.48 1906 100.00 100.00 7.29e-03 EMBL CAA37057 'myosin light chain kinase [Gallus gallus]' 90.48 972 100.00 100.00 2.86e-03 PDB 2O5G 'Calmodulin-Smooth Muscle Light Chain Kinase Peptide Complex' 90.48 19 100.00 100.00 1.02e-01 DBJ BAF80632 'myosin light chain kinase 1 [Danio rerio]' 85.71 899 100.00 100.00 6.65e-03 PDB 1QTX 'The 1.65 Angstrom Structure Of Calmodulin Rs20 Peptide Complex' 85.71 20 100.00 100.00 2.35e-01 PDB 2K0F 'Calmodulin Complexed With Calmodulin-Binding Peptide From Smooth Muscle Myosin Light Chain Kinase' 85.71 19 100.00 100.00 2.55e-01 BMRB 5227 'Calmodulin recognition element from smooth muscle myosin light chain kinase' 100.00 21 100.00 100.00 8.98e-03 PDB 1CDL 'Target Enzyme Recognition By Calmodulin: 2.4 Angstroms Structure Of A Calmodulin-Peptide Complex' 90.48 20 100.00 100.00 9.21e-02 BMRB 15184 CaMK1p 90.48 20 100.00 100.00 9.21e-02 BMRB 15186 smMLCKp 100.00 21 100.00 100.00 8.98e-03 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Calmodulin Chicken 9031 Eukaryota Metazoa Gallus gallus $SMMLCK Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Calmodulin 'recombinant technology' . . . . . $SMMLCK 'recombinant technology' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_one _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $Calmodulin 1.2 mM . . '[U-13C; U-15N]' $SMMLCK 2.4 mM . . . imidazole . mM 10 20 [U-2H] KCl 100 mM . . . CaCl2 6 mM . . . NaN3 0.02 % . . . H2O 93 % . . . D2O 7 % . . . stop_ save_ save_sample_two _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $Calmodulin 1.2 mM . . '[U-50% 2H; U-13C; U-15N]' $SMMLCK 2.4 mM . . . bis-tris . mM 10 20 [U-2H] KCl 100 mM . . . CaCl2 6 mM . . . NaN3 0.02 % . . . H2O 93 % . . . D2O 7 % . . . stop_ save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model Inova _Field_strength 600 _Details . save_ save_NMR_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model Inova _Field_strength 750 _Details . save_ ############################# # NMR applied experiments # ############################# save_15N_T1_1 _Saveframe_category NMR_applied_experiment _Experiment_name '15N T1' _Sample_label . save_ save_15N_T2_2 _Saveframe_category NMR_applied_experiment _Experiment_name '15N T2' _Sample_label . save_ save_15N_T1rho_3 _Saveframe_category NMR_applied_experiment _Experiment_name '15N T1rho' _Sample_label . save_ save_{1H}-15N_NOE_4 _Saveframe_category NMR_applied_experiment _Experiment_name '{1H}-15N NOE' _Sample_label . save_ save_NMR_spec_expt__0_1 _Saveframe_category NMR_applied_experiment _Experiment_name '15N T1' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_2 _Saveframe_category NMR_applied_experiment _Experiment_name '15N T2' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_3 _Saveframe_category NMR_applied_experiment _Experiment_name '15N T1rho' _BMRB_pulse_sequence_accession_number . _Details . save_ save_NMR_spec_expt__0_4 _Saveframe_category NMR_applied_experiment _Experiment_name '{1H}-15N NOE' _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 0.05 pH temperature 295 0.2 K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 0.05 pH temperature 308 0.2 K stop_ save_ save_sample_conditions_3 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 0.05 pH temperature 320 0.2 K stop_ save_ save_sample_conditions_4 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 0.05 pH temperature 333 0.2 K stop_ save_ save_sample_conditions_5 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 0.05 pH temperature 346 0.2 K stop_ save_ save_sample_conditions_6 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 0.05 pH temperature 288 0.2 K stop_ save_ save_sample_conditions_7 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 0.05 pH temperature 301 0.2 K stop_ save_ save_sample_conditions_8 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 0.05 pH temperature 311 0.2 K stop_ save_ save_sample_conditions_9 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 0.05 pH temperature 314 0.2 K stop_ save_ save_sample_conditions_10 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 0.05 pH temperature 317 0.2 K stop_ save_ save_sample_conditions_11 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 0.05 pH temperature 323 0.2 K stop_ save_ save_sample_conditions_12 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 0.05 pH temperature 326 0.2 K stop_ save_ save_sample_conditions_13 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 5.0 0.05 pH temperature 340 0.2 K stop_ save_ save_15N_T1_22C _Saveframe_category T1_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 500 _T1_coherence_type Nz _T1_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T1_ID _Residue_seq_code _Residue_label _Atom_name _T1_value _T1_value_error 1 4 LEU N 0.735 0.007 2 5 THR N 0.720 0.013 3 6 GLU N 0.665 0.011 4 7 GLU N 0.685 0.010 5 8 GLN N 0.713 0.012 6 11 GLU N 0.708 0.011 7 13 LYS N 0.686 0.013 8 16 PHE N 0.694 0.015 9 18 LEU N 0.697 0.012 10 19 PHE N 0.701 0.016 11 20 ASP N 0.740 0.016 12 21 LYS N 0.744 0.013 13 23 GLY N 0.718 0.012 14 24 ASP N 0.707 0.011 15 26 THR N 0.699 0.011 16 27 ILE N 0.742 0.024 17 28 THR N 0.713 0.021 18 29 THR N 0.724 0.014 19 31 GLU N 0.765 0.019 20 33 GLY N 0.731 0.018 21 34 THR N 0.753 0.013 22 36 MET N 0.737 0.016 23 37 ARG N 0.723 0.016 24 39 LEU N 0.770 0.014 25 40 GLY N 0.728 0.012 26 42 ASN N 0.810 0.027 27 44 THR N 0.708 0.015 28 45 GLU N 0.682 0.012 29 47 GLU N 0.731 0.011 30 49 GLN N 0.692 0.010 31 52 ILE N 0.714 0.013 32 53 ASN N 0.706 0.011 33 54 GLU N 0.713 0.008 34 55 VAL N 0.701 0.014 35 57 ALA N 0.937 0.014 36 60 ASP N 0.739 0.010 37 62 THR N 0.716 0.009 38 63 ILE N 0.703 0.020 39 64 ASP N 0.719 0.019 40 65 PHE N 0.662 0.023 41 67 GLU N 0.664 0.028 42 69 LEU N 0.665 0.016 43 70 THR N 0.759 0.013 44 71 MET N 0.694 0.014 45 74 ARG N 0.699 0.013 46 75 LYS N 0.694 0.016 47 77 LYS N 0.677 0.021 48 80 ASP N 0.698 0.020 49 81 SER N 0.654 0.013 50 87 GLU N 0.700 0.013 51 89 PHE N 0.699 0.014 52 91 VAL N 0.724 0.011 53 92 PHE N 0.727 0.017 54 93 ASP N 0.748 0.015 55 94 LYS N 0.741 0.013 56 96 GLY N 0.695 0.012 57 100 ILE N 0.771 0.024 58 101 SER N 0.706 0.023 59 102 ALA N 0.749 0.014 60 105 LEU N 0.727 0.015 61 106 ARG N 0.727 0.014 62 109 MET N 0.742 0.016 63 110 THR N 0.719 0.016 64 111 ASN N 0.722 0.013 65 112 LEU N 0.762 0.014 66 113 GLY N 0.733 0.014 67 115 LYS N 0.704 0.009 68 116 LEU N 0.745 0.007 69 117 THR N 0.696 0.015 70 118 ASP N 0.670 0.016 71 120 GLU N 0.714 0.013 72 123 GLU N 0.685 0.009 73 125 ILE N 0.700 0.016 74 126 ARG N 0.683 0.011 75 127 GLU N 0.713 0.011 76 128 ALA N 0.699 0.012 77 129 ASP N 0.731 0.012 78 130 ILE N 0.974 0.020 79 131 ASP N 0.726 0.012 80 133 ASP N 0.738 0.011 81 134 GLY N 0.703 0.013 82 135 GLN N 0.735 0.009 83 136 VAL N 0.704 0.016 84 137 ASN N 0.729 0.021 85 139 GLU N 0.736 0.010 86 143 GLN N 0.702 0.010 87 144 MET N 0.690 0.012 88 145 MET N 0.713 0.016 89 146 THR N 0.761 0.013 90 147 ALA N 0.611 0.007 91 148 LYS N 0.685 0.003 stop_ save_ save_15N_T1_35C _Saveframe_category T1_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_2 _Spectrometer_frequency_1H 500 _T1_coherence_type Nz _T1_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T1_ID _Residue_seq_code _Residue_label _Atom_name _T1_value _T1_value_error 1 4 LEU N 0.684 0.009 2 5 THR N 0.583 0.008 3 6 GLU N 0.550 0.013 4 7 GLU N 0.536 0.010 5 8 GLN N 0.554 0.008 6 9 ILE N 0.547 0.008 7 11 GLU N 0.548 0.007 8 12 PHE N 0.534 0.007 9 13 LYS N 0.524 0.007 10 15 ALA N 0.540 0.007 11 16 PHE N 0.541 0.009 12 18 LEU N 0.548 0.008 13 19 PHE N 0.546 0.009 14 20 ASP N 0.587 0.010 15 21 LYS N 0.581 0.008 16 23 GLY N 0.559 0.008 17 24 ASP N 0.546 0.008 18 26 THR N 0.539 0.008 19 27 ILE N 0.561 0.013 20 28 THR N 0.550 0.013 21 29 THR N 0.568 0.011 22 30 LYS N 0.567 0.014 23 31 GLU N 0.581 0.011 24 32 LEU N 0.560 0.009 25 33 GLY N 0.564 0.010 26 36 MET N 0.554 0.009 27 37 ARG N 0.543 0.009 28 39 LEU N 0.590 0.008 29 40 GLY N 0.575 0.008 30 42 ASN N 0.661 0.067 31 44 THR N 0.567 0.009 32 45 GLU N 0.525 0.011 33 47 GLU N 0.571 0.008 34 49 GLN N 0.544 0.007 35 51 MET N 0.568 0.007 36 52 ILE N 0.543 0.008 37 53 ASN N 0.540 0.006 38 54 GLU N 0.548 0.005 39 55 VAL N 0.561 0.009 40 57 ALA N 0.747 0.010 41 60 ASP N 0.568 0.007 42 62 THR N 0.551 0.007 43 63 ILE N 0.533 0.011 44 64 ASP N 0.550 0.011 45 65 PHE N 0.530 0.012 46 67 GLU N 0.548 0.014 47 68 PHE N 0.534 0.011 48 69 LEU N 0.528 0.010 49 70 THR N 0.560 0.008 50 71 MET N 0.541 0.009 51 74 ARG N 0.578 0.008 52 75 LYS N 0.619 0.021 53 77 LYS N 0.626 0.032 54 80 ASP N 0.566 0.017 55 81 SER N 0.543 0.020 56 85 ILE N 0.545 0.008 57 86 ARG N 0.531 0.008 58 87 GLU N 0.545 0.008 59 89 PHE N 0.535 0.008 60 91 VAL N 0.559 0.007 61 92 PHE N 0.558 0.010 62 93 ASP N 0.572 0.009 63 94 LYS N 0.573 0.007 64 96 GLY N 0.541 0.008 65 97 ASN N 0.548 0.008 66 100 ILE N 0.573 0.012 67 101 SER N 0.535 0.012 68 102 ALA N 0.574 0.009 69 103 ALA N 0.544 0.009 70 105 LEU N 0.573 0.009 71 106 ARG N 0.553 0.008 72 109 MET N 0.571 0.009 73 110 THR N 0.558 0.010 74 111 ASN N 0.557 0.008 75 112 LEU N 0.598 0.008 76 113 GLY N 0.577 0.009 77 114 GLU N 0.546 0.007 78 115 LYS N 0.644 0.011 79 116 LEU N 0.677 0.007 80 117 THR N 0.570 0.009 81 118 ASP N 0.580 0.026 82 119 GLU N 0.518 0.009 83 120 GLU N 0.557 0.008 84 123 GLU N 0.532 0.006 85 125 ILE N 0.542 0.008 86 126 ARG N 0.530 0.006 87 127 GLU N 0.552 0.007 88 128 ALA N 0.545 0.007 89 130 ILE N 0.756 0.011 90 131 ASP N 0.573 0.008 91 133 ASP N 0.570 0.008 92 134 GLY N 0.548 0.009 93 135 GLN N 0.556 0.006 94 136 VAL N 0.550 0.009 95 137 ASN N 0.548 0.011 96 138 TYR N 0.543 0.009 97 139 GLU N 0.560 0.006 98 141 PHE N 0.525 0.009 99 142 VAL N 0.531 0.007 100 143 GLN N 0.551 0.006 101 144 MET N 0.542 0.007 102 145 MET N 0.558 0.009 103 146 THR N 0.586 0.009 104 147 ALA N 0.538 0.009 105 148 LYS N 0.676 0.003 stop_ save_ save_15N_T1_47C _Saveframe_category T1_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_3 _Spectrometer_frequency_1H 500 _T1_coherence_type Nz _T1_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T1_ID _Residue_seq_code _Residue_label _Atom_name _T1_value _T1_value_error 1 4 LEU N 0.707 0.014 2 5 THR N 0.493 0.006 3 6 GLU N 0.488 0.024 4 7 GLU N 0.464 0.012 5 8 GLN N 0.456 0.004 6 9 ILE N 0.458 0.004 7 12 PHE N 0.452 0.004 8 13 LYS N 0.444 0.004 9 15 ALA N 0.454 0.004 10 16 PHE N 0.451 0.005 11 18 LEU N 0.446 0.004 12 19 PHE N 0.462 0.005 13 21 LYS N 0.501 0.004 14 23 GLY N 0.457 0.004 15 25 GLY N 0.467 0.006 16 26 THR N 0.447 0.005 17 27 ILE N 0.471 0.007 18 28 THR N 0.453 0.007 19 29 THR N 0.471 0.010 20 30 LYS N 0.479 0.027 21 31 GLU N 0.469 0.005 22 32 LEU N 0.465 0.004 23 33 GLY N 0.454 0.005 24 34 THR N 0.469 0.004 25 37 ARG N 0.459 0.004 26 39 LEU N 0.494 0.005 27 40 GLY N 0.480 0.005 28 44 THR N 0.481 0.005 29 45 GLU N 0.492 0.022 30 47 GLU N 0.468 0.004 31 49 GLN N 0.450 0.003 32 51 MET N 0.457 0.004 33 52 ILE N 0.465 0.004 34 55 VAL N 0.462 0.005 35 57 ALA N 0.635 0.005 36 59 GLY N 0.509 0.005 37 60 ASP N 0.465 0.005 38 61 GLY N 0.456 0.005 39 62 THR N 0.452 0.004 40 63 ILE N 0.452 0.006 41 64 ASP N 0.454 0.006 42 65 PHE N 0.433 0.007 43 67 GLU N 0.451 0.006 44 69 LEU N 0.441 0.005 45 70 THR N 0.468 0.004 46 71 MET N 0.447 0.004 47 74 ARG N 0.495 0.004 48 75 LYS N 0.548 0.046 49 85 ILE N 0.447 0.004 50 87 GLU N 0.446 0.004 51 89 PHE N 0.444 0.004 52 91 VAL N 0.461 0.004 53 92 PHE N 0.468 0.005 54 93 ASP N 0.472 0.005 55 94 LYS N 0.470 0.004 56 96 GLY N 0.450 0.004 57 97 ASN N 0.453 0.005 58 98 GLY N 0.458 0.006 59 100 ILE N 0.471 0.007 60 101 SER N 0.452 0.007 61 102 ALA N 0.467 0.005 62 106 ARG N 0.457 0.004 63 109 MET N 0.466 0.004 64 111 ASN N 0.453 0.004 65 112 LEU N 0.488 0.004 66 113 GLY N 0.490 0.005 67 114 GLU N 0.460 0.004 68 115 LYS N 0.653 0.019 69 116 LEU N 0.622 0.005 70 117 THR N 0.505 0.005 71 119 GLU N 0.461 0.010 72 120 GLU N 0.458 0.004 73 123 GLU N 0.440 0.003 74 125 ILE N 0.439 0.004 75 126 ARG N 0.440 0.003 76 127 GLU N 0.460 0.003 77 128 ALA N 0.460 0.004 78 129 ASP N 0.455 0.004 79 130 ILE N 0.634 0.006 80 132 GLY N 0.518 0.005 81 133 ASP N 0.468 0.004 82 134 GLY N 0.452 0.005 83 135 GLN N 0.462 0.004 84 136 VAL N 0.465 0.005 85 137 ASN N 0.456 0.006 86 138 TYR N 0.450 0.005 87 142 VAL N 0.438 0.004 88 143 GLN N 0.450 0.003 89 144 MET N 0.459 0.004 90 145 MET N 0.466 0.005 91 146 THR N 0.496 0.006 92 147 ALA N 0.532 0.014 93 148 LYS N 0.693 0.003 stop_ save_ save_15N_T1_60C _Saveframe_category T1_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_4 _Spectrometer_frequency_1H 500 _T1_coherence_type Nz _T1_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T1_ID _Residue_seq_code _Residue_label _Atom_name _T1_value _T1_value_error 1 4 LEU N 0.810 0.052 2 5 THR N 0.479 0.009 3 7 GLU N 0.427 0.028 4 8 GLN N 0.431 0.006 5 9 ILE N 0.408 0.004 6 11 GLU N 0.411 0.003 7 12 PHE N 0.413 0.004 8 15 ALA N 0.406 0.003 9 16 PHE N 0.394 0.004 10 19 PHE N 0.420 0.005 11 21 LYS N 0.452 0.004 12 23 GLY N 0.416 0.004 13 25 GLY N 0.404 0.005 14 26 THR N 0.401 0.004 15 27 ILE N 0.412 0.006 16 28 THR N 0.405 0.006 17 29 THR N 0.451 0.028 18 31 GLU N 0.420 0.005 19 32 LEU N 0.407 0.004 20 33 GLY N 0.398 0.004 21 37 ARG N 0.407 0.004 22 39 LEU N 0.439 0.004 23 40 GLY N 0.430 0.006 24 44 THR N 0.444 0.005 25 47 GLU N 0.419 0.003 26 49 GLN N 0.413 0.003 27 51 MET N 0.417 0.003 28 52 ILE N 0.419 0.004 29 55 VAL N 0.420 0.005 30 57 ALA N 0.564 0.005 31 59 GLY N 0.448 0.005 32 60 ASP N 0.417 0.004 33 61 GLY N 0.415 0.005 34 62 THR N 0.403 0.004 35 63 ILE N 0.399 0.005 36 64 ASP N 0.408 0.006 37 65 PHE N 0.407 0.008 38 67 GLU N 0.402 0.005 39 69 LEU N 0.398 0.005 40 70 THR N 0.408 0.004 41 74 ARG N 0.461 0.007 42 87 GLU N 0.401 0.004 43 89 PHE N 0.400 0.004 44 91 VAL N 0.414 0.003 45 92 PHE N 0.418 0.004 46 93 ASP N 0.426 0.004 47 94 LYS N 0.438 0.003 48 96 GLY N 0.412 0.004 49 97 ASN N 0.402 0.004 50 98 GLY N 0.413 0.006 51 100 ILE N 0.419 0.006 52 102 ALA N 0.410 0.006 53 106 ARG N 0.401 0.003 54 109 MET N 0.402 0.003 55 111 ASN N 0.404 0.004 56 112 LEU N 0.436 0.004 57 113 GLY N 0.432 0.005 58 114 GLU N 0.426 0.004 59 115 LYS N 0.670 0.054 60 116 LEU N 0.634 0.010 61 117 THR N 0.462 0.005 62 120 GLU N 0.414 0.003 63 123 GLU N 0.402 0.003 64 125 ILE N 0.402 0.004 65 126 ARG N 0.386 0.003 66 127 GLU N 0.404 0.003 67 128 ALA N 0.408 0.004 68 129 ASP N 0.419 0.003 69 130 ILE N 0.567 0.005 70 132 GLY N 0.453 0.004 71 133 ASP N 0.427 0.004 72 134 GLY N 0.408 0.004 73 135 GLN N 0.413 0.004 74 136 VAL N 0.415 0.004 75 137 ASN N 0.410 0.005 76 138 TYR N 0.410 0.005 77 142 VAL N 0.398 0.003 78 143 GLN N 0.407 0.003 79 144 MET N 0.413 0.004 80 145 MET N 0.426 0.004 81 146 THR N 0.459 0.006 82 147 ALA N 0.538 0.042 83 148 LYS N 0.732 0.005 stop_ save_ save_15N_T1_73C _Saveframe_category T1_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_5 _Spectrometer_frequency_1H 500 _T1_coherence_type Nz _T1_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T1_ID _Residue_seq_code _Residue_label _Atom_name _T1_value _T1_value_error 1 8 GLN N 0.46754 0.01331 2 9 ILE N 0.43277 0.00654 3 11 GLU N 0.43557 0.00634 4 12 PHE N 0.43543 0.00625 5 15 ALA N 0.43225 0.00509 6 16 PHE N 0.44186 0.00477 7 19 PHE N 0.45600 0.00577 8 21 LYS N 0.50958 0.00530 9 23 GLY N 0.45827 0.00492 10 25 GLY N 0.43969 0.00644 11 27 ILE N 0.45395 0.00756 12 28 THR N 0.44519 0.00614 13 32 LEU N 0.44631 0.00458 14 33 GLY N 0.43620 0.00532 15 39 LEU N 0.46539 0.00503 16 40 GLY N 0.46257 0.01110 17 44 THR N 0.48661 0.00730 18 47 GLU N 0.46049 0.00425 19 49 GLN N 0.44195 0.00352 20 52 ILE N 0.44287 0.00445 21 55 VAL N 0.45311 0.00563 22 57 ALA N 0.60608 0.00733 23 59 GLY N 0.46358 0.00648 24 61 GLY N 0.43622 0.00628 25 62 THR N 0.43632 0.00499 26 63 ILE N 0.44699 0.00602 27 64 ASP N 0.43420 0.00756 28 65 PHE N 0.43254 0.01497 29 67 GLU N 0.43210 0.00723 30 69 LEU N 0.42652 0.00717 31 70 THR N 0.43873 0.00601 32 74 ARG N 0.45663 0.02560 33 89 PHE N 0.43471 0.00514 34 91 VAL N 0.44645 0.00453 35 92 PHE N 0.44751 0.00597 36 93 ASP N 0.45782 0.00542 37 94 LYS N 0.48900 0.00499 38 96 GLY N 0.44725 0.00503 39 97 ASN N 0.44039 0.00504 40 98 GLY N 0.42663 0.00652 41 100 ILE N 0.45822 0.00804 42 102 ALA N 0.45302 0.01403 43 106 ARG N 0.45484 0.00411 44 109 MET N 0.44988 0.00435 45 111 ASN N 0.42993 0.00614 46 112 LEU N 0.46096 0.00519 47 113 GLY N 0.47836 0.00938 48 114 GLU N 0.45777 0.00706 49 116 LEU N 0.72836 0.02873 50 117 THR N 0.50542 0.00938 51 120 GLU N 0.44677 0.00437 52 123 GLU N 0.43775 0.00336 53 125 ILE N 0.43929 0.00620 54 127 GLU N 0.43412 0.00479 55 128 ALA N 0.44541 0.00557 56 129 ASP N 0.45532 0.00445 57 130 ILE N 0.60610 0.00623 58 132 GLY N 0.48623 0.00538 59 133 ASP N 0.44713 0.00452 60 134 GLY N 0.44908 0.00606 61 135 GLN N 0.43807 0.00414 62 136 VAL N 0.44711 0.00489 63 137 ASN N 0.43820 0.00746 64 138 TYR N 0.42991 0.00556 65 142 VAL N 0.43781 0.00405 66 143 GLN N 0.44486 0.00370 67 144 MET N 0.44976 0.00498 68 145 MET N 0.46263 0.00564 69 146 THR N 0.46776 0.00867 70 148 LYS N 0.77130 0.01259 stop_ save_ save_15N_T2_22C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 500 _T2_coherence_type Nz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU N 0.1141 0.0014 . . 2 5 THR N 0.0775 0.0015 . . 3 6 GLU N 0.0740 0.0013 . . 4 7 GLU N 0.0711 0.0011 . . 5 8 GLN N 0.0708 0.0014 . . 6 11 GLU N 0.0691 0.0013 . . 7 13 LYS N 0.0682 0.0013 . . 8 16 PHE N 0.0664 0.0015 . . 9 18 LEU N 0.0650 0.0013 . . 10 19 PHE N 0.0703 0.0019 . . 11 20 ASP N 0.0697 0.0018 . . 12 21 LYS N 0.0760 0.0016 . . 13 23 GLY N 0.0735 0.0014 . . 14 24 ASP N 0.0695 0.0012 . . 15 26 THR N 0.0676 0.0012 . . 16 27 ILE N 0.0706 0.0026 . . 17 28 THR N 0.0679 0.0022 . . 18 29 THR N 0.0652 0.0014 . . 19 31 GLU N 0.0637 0.0018 . . 20 33 GLY N 0.0655 0.0016 . . 21 34 THR N 0.0668 0.0013 . . 22 36 MET N 0.0635 0.0015 . . 23 37 ARG N 0.0649 0.0015 . . 24 39 LEU N 0.0655 0.0013 . . 25 40 GLY N 0.0754 0.0014 . . 26 42 ASN N 0.0835 0.0028 . . 27 44 THR N 0.0756 0.0017 . . 28 45 GLU N 0.0707 0.0013 . . 29 47 GLU N 0.0681 0.0012 . . 30 49 GLN N 0.0687 0.0011 . . 31 52 ILE N 0.0687 0.0015 . . 32 53 ASN N 0.0675 0.0011 . . 33 54 GLU N 0.0702 0.0010 . . 34 55 VAL N 0.0701 0.0016 . . 35 57 ALA N 0.0908 0.0016 . . 36 60 ASP N 0.0677 0.0010 . . 37 62 THR N 0.0676 0.0010 . . 38 63 ILE N 0.0682 0.0021 . . 39 64 ASP N 0.0707 0.0020 . . 40 65 PHE N 0.0588 0.0021 . . 41 67 GLU N 0.0647 0.0031 . . 42 69 LEU N 0.0651 0.0017 . . 43 70 THR N 0.0657 0.0013 . . 44 71 MET N 0.0669 0.0016 . . 45 74 ARG N 0.0747 0.0017 . . 46 75 LYS N 0.0625 0.0015 . . 47 77 LYS N 0.0748 0.0024 . . 48 80 ASP N 0.0809 0.0028 . . 49 81 SER N 0.0699 0.0015 . . 50 87 GLU N 0.0664 0.0014 . . 51 89 PHE N 0.0669 0.0015 . . 52 91 VAL N 0.0685 0.0013 . . 53 92 PHE N 0.0673 0.0018 . . 54 93 ASP N 0.0697 0.0015 . . 55 94 LYS N 0.0739 0.0015 . . 56 96 GLY N 0.0735 0.0014 . . 57 100 ILE N 0.0735 0.0026 . . 58 101 SER N 0.0651 0.0022 . . 59 102 ALA N 0.0646 0.0013 . . 60 105 LEU N 0.0627 0.0014 . . 61 106 ARG N 0.0646 0.0013 . . 62 109 MET N 0.0619 0.0015 . . 63 110 THR N 0.0671 0.0017 . . 64 111 ASN N 0.0667 0.0014 . . 65 112 LEU N 0.0647 0.0013 . . 66 113 GLY N 0.0722 0.0017 . . 67 115 LYS N 0.1083 0.0016 . . 68 116 LEU N 0.1149 0.0015 . . 69 117 THR N 0.0798 0.0019 . . 70 118 ASP N 0.0721 0.0018 . . 71 120 GLU N 0.0716 0.0015 . . 72 123 GLU N 0.0711 0.0011 . . 73 125 ILE N 0.0667 0.0017 . . 74 126 ARG N 0.0694 0.0012 . . 75 127 GLU N 0.0682 0.0012 . . 76 128 ALA N 0.0689 0.0014 . . 77 129 ASP N 0.0712 0.0013 . . 78 130 ILE N 0.0967 0.0022 . . 79 131 ASP N 0.0674 0.0012 . . 80 133 ASP N 0.0670 0.0010 . . 81 134 GLY N 0.0707 0.0016 . . 82 135 GLN N 0.0674 0.0010 . . 83 136 VAL N 0.0722 0.0018 . . 84 137 ASN N 0.0699 0.0021 . . 85 139 GLU N 0.0690 0.0010 . . 86 143 GLN N 0.0653 0.0011 . . 87 144 MET N 0.0680 0.0015 . . 88 145 MET N 0.0690 0.0018 . . 89 146 THR N 0.0741 0.0015 . . 90 147 ALA N 0.1060 0.0015 . . 91 148 LYS N 0.1784 0.0014 . . stop_ save_ save_15N_T2_35C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_2 _Spectrometer_frequency_1H 500 _T2_coherence_type Nz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU N 0.1552 0.0031 . . 2 5 THR N 0.1128 0.0022 . . 3 6 GLU N 0.0985 0.0027 . . 4 7 GLU N 0.0932 0.0020 . . 5 8 GLN N 0.1017 0.0020 . . 6 9 ILE N 0.0976 0.0019 . . 7 11 GLU N 0.0960 0.0015 . . 8 12 PHE N 0.0933 0.0017 . . 9 13 LYS N 0.0956 0.0018 . . 10 15 ALA N 0.0946 0.0017 . . 11 16 PHE N 0.0935 0.0020 . . 12 18 LEU N 0.0910 0.0017 . . 13 19 PHE N 0.0995 0.0025 . . 14 20 ASP N 0.0990 0.0024 . . 15 21 LYS N 0.1120 0.0023 . . 16 23 GLY N 0.1034 0.0021 . . 17 24 ASP N 0.0957 0.0018 . . 18 26 THR N 0.0929 0.0018 . . 19 27 ILE N 0.1001 0.0033 . . 20 28 THR N 0.0930 0.0027 . . 21 29 THR N 0.0930 0.0023 . . 22 30 LYS N 0.0900 0.0028 . . 23 31 GLU N 0.0911 0.0023 . . 24 32 LEU N 0.0865 0.0016 . . 25 33 GLY N 0.0923 0.0021 . . 26 36 MET N 0.0871 0.0017 . . 27 37 ARG N 0.0909 0.0018 . . 28 39 LEU N 0.0912 0.0017 . . 29 40 GLY N 0.1111 0.0024 . . 30 42 ASN N 0.1187 0.0155 . . 31 44 THR N 0.1085 0.0024 . . 32 45 GLU N 0.0949 0.0025 . . 33 47 GLU N 0.0966 0.0018 . . 34 49 GLN N 0.0974 0.0016 . . 35 51 MET N 0.0965 0.0016 . . 36 52 ILE N 0.0953 0.0018 . . 37 53 ASN N 0.0949 0.0015 . . 38 54 GLU N 0.0925 0.0012 . . 39 55 VAL N 0.1045 0.0024 . . 40 57 ALA N 0.1266 0.0024 . . 41 60 ASP N 0.0949 0.0017 . . 42 62 THR N 0.0946 0.0016 . . 43 63 ILE N 0.0945 0.0026 . . 44 64 ASP N 0.0980 0.0027 . . 45 65 PHE N 0.0851 0.0024 . . 46 67 GLU N 0.0928 0.0031 . . 47 68 PHE N 0.0935 0.0025 . . 48 69 LEU N 0.0939 0.0022 . . 49 70 THR N 0.0937 0.0018 . . 50 71 MET N 0.0912 0.0020 . . 51 74 ARG N 0.1111 0.0021 . . 52 75 LYS N 0.0894 0.0035 . . 53 77 LYS N 0.1011 0.0060 . . 54 80 ASP N 0.1148 0.0049 . . 55 81 SER N 0.0905 0.0038 . . 56 85 ILE N 0.0941 0.0019 . . 57 86 ARG N 0.0965 0.0018 . . 58 87 GLU N 0.0951 0.0018 . . 59 89 PHE N 0.0934 0.0019 . . 60 91 VAL N 0.0928 0.0015 . . 61 92 PHE N 0.0983 0.0025 . . 62 93 ASP N 0.0955 0.0020 . . 63 94 LYS N 0.1005 0.0018 . . 64 96 GLY N 0.1009 0.0020 . . 65 97 ASN N 0.0957 0.0017 . . 66 100 ILE N 0.1020 0.0032 . . 67 101 SER N 0.0929 0.0028 . . 68 102 ALA N 0.0923 0.0018 . . 69 103 ALA N 0.0885 0.0017 . . 70 105 LEU N 0.0896 0.0017 . . 71 106 ARG N 0.0878 0.0015 . . 72 109 MET N 0.0893 0.0018 . . 73 110 THR N 0.0930 0.0021 . . 74 111 ASN N 0.0895 0.0017 . . 75 112 LEU N 0.0909 0.0017 . . 76 113 GLY N 0.1002 0.0023 . . 77 114 GLU N 0.1018 0.0017 . . 78 115 LYS N 0.1411 0.0036 . . 79 116 LEU N 0.1677 0.0028 . . 80 117 THR N 0.1172 0.0028 . . 81 118 ASP N 0.0917 0.0046 . . 82 119 GLU N 0.0976 0.0020 . . 83 120 GLU N 0.0963 0.0018 . . 84 123 GLU N 0.0950 0.0013 . . 85 125 ILE N 0.0922 0.0018 . . 86 126 ARG N 0.0918 0.0014 . . 87 127 GLU N 0.0961 0.0015 . . 88 128 ALA N 0.0967 0.0018 . . 89 130 ILE N 0.1415 0.0034 . . 90 131 ASP N 0.0930 0.0017 . . 91 133 ASP N 0.0932 0.0016 . . 92 134 GLY N 0.0958 0.0022 . . 93 135 GLN N 0.0932 0.0014 . . 94 136 VAL N 0.0983 0.0022 . . 95 137 ASN N 0.0981 0.0029 . . 96 138 TYR N 0.0936 0.0020 . . 97 139 GLU N 0.0962 0.0014 . . 98 141 PHE N 0.0951 0.0022 . . 99 142 VAL N 0.0905 0.0015 . . 100 143 GLN N 0.0902 0.0014 . . 101 144 MET N 0.0957 0.0018 . . 102 145 MET N 0.0975 0.0022 . . 103 146 THR N 0.1099 0.0026 . . 104 147 ALA N 0.1454 0.0037 . . 105 148 LYS N 0.2443 0.0029 . . stop_ save_ save_15N_T2_47C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_3 _Spectrometer_frequency_1H 500 _T2_coherence_type Nz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU N 0.2152 0.0082 . . 2 5 THR N 0.1451 0.0026 . . 3 6 GLU N 0.1274 0.0084 . . 4 7 GLU N 0.1268 0.0046 . . 5 8 GLN N 0.1334 0.0020 . . 6 9 ILE N 0.1294 0.0017 . . 7 12 PHE N 0.1206 0.0016 . . 8 13 LYS N 0.1275 0.0016 . . 9 15 ALA N 0.1271 0.0016 . . 10 16 PHE N 0.1269 0.0019 . . 11 18 LEU N 0.1191 0.0015 . . 12 19 PHE N 0.1319 0.0024 . . 13 21 LYS N 0.1383 0.0018 . . 14 23 GLY N 0.1377 0.0020 . . 15 25 GLY N 0.1260 0.0028 . . 16 26 THR N 0.1291 0.0020 . . 17 27 ILE N 0.1292 0.0030 . . 18 28 THR N 0.1213 0.0025 . . 19 29 THR N 0.1157 0.0033 . . 20 30 LYS N 0.1142 0.0089 . . 21 31 GLU N 0.1208 0.0021 . . 22 32 LEU N 0.1123 0.0015 . . 23 33 GLY N 0.1213 0.0019 . . 24 34 THR N 0.1272 0.0017 . . 25 37 ARG N 0.1237 0.0017 . . 26 39 LEU N 0.1210 0.0017 . . 27 40 GLY N 0.1428 0.0027 . . 28 44 THR N 0.1425 0.0023 . . 29 45 GLU N 0.1225 0.0070 . . 30 47 GLU N 0.1313 0.0018 . . 31 49 GLN N 0.1241 0.0013 . . 32 51 MET N 0.1289 0.0015 . . 33 52 ILE N 0.1281 0.0017 . . 34 55 VAL N 0.1360 0.0024 . . 35 57 ALA N 0.1798 0.0027 . . 36 59 GLY N 0.1398 0.0023 . . 37 60 ASP N 0.1280 0.0019 . . 38 61 GLY N 0.1289 0.0024 . . 39 62 THR N 0.1209 0.0016 . . 40 63 ILE N 0.1241 0.0025 . . 41 64 ASP N 0.1265 0.0027 . . 42 65 PHE N 0.1171 0.0026 . . 43 67 GLU N 0.1239 0.0026 . . 44 69 LEU N 0.1219 0.0020 . . 45 70 THR N 0.1257 0.0018 . . 46 71 MET N 0.1280 0.0020 . . 47 74 ARG N 0.1483 0.0023 . . 48 75 LYS N 0.1128 0.0123 . . 49 85 ILE N 0.1255 0.0018 . . 50 87 GLU N 0.1203 0.0014 . . 51 89 PHE N 0.1202 0.0016 . . 52 91 VAL N 0.1295 0.0017 . . 53 92 PHE N 0.1297 0.0022 . . 54 93 ASP N 0.1258 0.0018 . . 55 94 LYS N 0.1397 0.0017 . . 56 96 GLY N 0.1335 0.0020 . . 57 97 ASN N 0.1305 0.0020 . . 58 98 GLY N 0.1220 0.0027 . . 59 100 ILE N 0.1374 0.0034 . . 60 101 SER N 0.1227 0.0027 . . 61 102 ALA N 0.1234 0.0020 . . 62 106 ARG N 0.1169 0.0013 . . 63 109 MET N 0.1132 0.0014 . . 64 111 ASN N 0.1213 0.0017 . . 65 112 LEU N 0.1225 0.0017 . . 66 113 GLY N 0.1326 0.0023 . . 67 114 GLU N 0.1362 0.0018 . . 68 115 LYS N 0.1896 0.0090 . . 69 116 LEU N 0.2110 0.0036 . . 70 117 THR N 0.1550 0.0027 . . 71 119 GLU N 0.1235 0.0037 . . 72 120 GLU N 0.1298 0.0016 . . 73 123 GLU N 0.1239 0.0012 . . 74 125 ILE N 0.1221 0.0016 . . 75 126 ARG N 0.1273 0.0013 . . 76 127 GLU N 0.1232 0.0013 . . 77 128 ALA N 0.1261 0.0016 . . 78 129 ASP N 0.1280 0.0015 . . 79 130 ILE N 0.1764 0.0029 . . 80 132 GLY N 0.1323 0.0019 . . 81 133 ASP N 0.1237 0.0017 . . 82 134 GLY N 0.1272 0.0022 . . 83 135 GLN N 0.1308 0.0017 . . 84 136 VAL N 0.1358 0.0023 . . 85 137 ASN N 0.1298 0.0028 . . 86 138 TYR N 0.1258 0.0020 . . 87 142 VAL N 0.1229 0.0015 . . 88 143 GLN N 0.1186 0.0013 . . 89 144 MET N 0.1316 0.0018 . . 90 145 MET N 0.1290 0.0020 . . 91 146 THR N 0.1399 0.0026 . . 92 147 ALA N 0.1942 0.0093 . . 93 148 LYS N 0.3668 0.0051 . . stop_ save_ save_15N_T2_60C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_4 _Spectrometer_frequency_1H 500 _T2_coherence_type Nz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU N 0.2464 0.0256 . . 2 5 THR N 0.1720 0.0048 . . 3 7 GLU N 0.1483 0.0118 . . 4 8 GLN N 0.1516 0.0028 . . 5 9 ILE N 0.1543 0.0022 . . 6 11 GLU N 0.1539 0.0020 . . 7 12 PHE N 0.1426 0.0020 . . 8 15 ALA N 0.1536 0.0020 . . 9 16 PHE N 0.1430 0.0018 . . 10 19 PHE N 0.1526 0.0027 . . 11 21 LYS N 0.1776 0.0025 . . 12 23 GLY N 0.1661 0.0022 . . 13 25 GLY N 0.1419 0.0025 . . 14 26 THR N 0.1469 0.0020 . . 15 27 ILE N 0.1517 0.0032 . . 16 28 THR N 0.1461 0.0028 . . 17 29 THR N 0.1340 0.0093 . . 18 31 GLU N 0.1412 0.0022 . . 19 32 LEU N 0.1381 0.0017 . . 20 33 GLY N 0.1401 0.0020 . . 21 37 ARG N 0.1438 0.0020 . . 22 39 LEU N 0.1525 0.0023 . . 23 40 GLY N 0.1738 0.0039 . . 24 44 THR N 0.1742 0.0031 . . 25 47 GLU N 0.1534 0.0019 . . 26 49 GLN N 0.1634 0.0021 . . 27 51 MET N 0.1456 0.0016 . . 28 52 ILE N 0.1438 0.0017 . . 29 55 VAL N 0.1549 0.0026 . . 30 57 ALA N 0.2112 0.0033 . . 31 59 GLY N 0.1625 0.0025 . . 32 60 ASP N 0.1511 0.0021 . . 33 61 GLY N 0.1559 0.0029 . . 34 62 THR N 0.1484 0.0020 . . 35 63 ILE N 0.1503 0.0029 . . 36 64 ASP N 0.1527 0.0032 . . 37 65 PHE N 0.1367 0.0034 . . 38 67 GLU N 0.1464 0.0026 . . 39 69 LEU N 0.1398 0.0022 . . 40 70 THR N 0.1459 0.0020 . . 41 74 ARG N 0.1657 0.0044 . . 42 87 GLU N 0.1359 0.0018 . . 43 89 PHE N 0.1474 0.0019 . . 44 91 VAL N 0.1457 0.0017 . . 45 92 PHE N 0.1507 0.0024 . . 46 93 ASP N 0.1468 0.0020 . . 47 94 LYS N 0.1591 0.0019 . . 48 96 GLY N 0.1524 0.0019 . . 49 97 ASN N 0.1551 0.0021 . . 50 98 GLY N 0.1406 0.0025 . . 51 100 ILE N 0.1529 0.0033 . . 52 102 ALA N 0.1387 0.0029 . . 53 106 ARG N 0.1355 0.0015 . . 54 109 MET N 0.1382 0.0016 . . 55 111 ASN N 0.1449 0.0021 . . 56 112 LEU N 0.1578 0.0024 . . 57 113 GLY N 0.1605 0.0030 . . 58 114 GLU N 0.1574 0.0023 . . 59 115 LYS N 0.2142 0.0239 . . 60 116 LEU N 0.2379 0.0070 . . 61 117 THR N 0.1765 0.0033 . . 62 120 GLU N 0.1574 0.0020 . . 63 123 GLU N 0.1480 0.0014 . . 64 125 ILE N 0.1389 0.0020 . . 65 126 ARG N 0.1413 0.0015 . . 66 127 GLU N 0.1489 0.0017 . . 67 128 ALA N 0.1467 0.0020 . . 68 129 ASP N 0.1513 0.0017 . . 69 130 ILE N 0.2059 0.0031 . . 70 132 GLY N 0.1603 0.0021 . . 71 133 ASP N 0.1441 0.0017 . . 72 134 GLY N 0.1545 0.0025 . . 73 135 GLN N 0.1481 0.0017 . . 74 136 VAL N 0.1565 0.0025 . . 75 137 ASN N 0.1519 0.0031 . . 76 138 TYR N 0.1441 0.0020 . . 77 142 VAL N 0.1494 0.0018 . . 78 143 GLN N 0.1391 0.0014 . . 79 144 MET N 0.1521 0.0021 . . 80 145 MET N 0.1492 0.0022 . . 81 146 THR N 0.1617 0.0030 . . 82 147 ALA N 0.2239 0.0286 . . 83 148 LYS N 0.3789 0.0080 . . stop_ save_ save_15N_T2_73C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_5 _Spectrometer_frequency_1H 500 _T2_coherence_type Nz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 8 GLN N 0.17105 0.01349 . . 2 9 ILE N 0.16369 0.00717 . . 3 11 GLU N 0.15479 0.00594 . . 4 12 PHE N 0.15096 0.00590 . . 5 15 ALA N 0.15707 0.00507 . . 6 16 PHE N 0.15495 0.00493 . . 7 19 PHE N 0.16948 0.00624 . . 8 21 LYS N 0.20601 0.00683 . . 9 23 GLY N 0.17768 0.00549 . . 10 25 GLY N 0.15316 0.00617 . . 11 27 ILE N 0.16847 0.00830 . . 12 28 THR N 0.16251 0.00716 . . 13 32 LEU N 0.14463 0.00425 . . 14 33 GLY N 0.14629 0.00538 . . 15 39 LEU N 0.15878 0.00484 . . 16 40 GLY N 0.17929 0.01240 . . 17 44 THR N 0.18563 0.00905 . . 18 47 GLU N 0.17131 0.00464 . . 19 49 GLN N 0.16334 0.00381 . . 20 52 ILE N 0.15480 0.00414 . . 21 55 VAL N 0.16450 0.00570 . . 22 57 ALA N 0.20007 0.00745 . . 23 59 GLY N 0.17350 0.00692 . . 24 61 GLY N 0.16492 0.00682 . . 25 62 THR N 0.15643 0.00484 . . 26 63 ILE N 0.16568 0.00729 . . 27 64 ASP N 0.15407 0.00791 . . 28 65 PHE N 0.14952 0.01451 . . 29 67 GLU N 0.15498 0.00645 . . 30 69 LEU N 0.14762 0.00647 . . 31 70 THR N 0.15493 0.00543 . . 32 74 ARG N 0.16561 0.02458 . . 33 89 PHE N 0.15411 0.00537 . . 34 91 VAL N 0.16351 0.00457 . . 35 92 PHE N 0.16353 0.00617 . . 36 93 ASP N 0.16604 0.00538 . . 37 94 LYS N 0.19251 0.00603 . . 38 96 GLY N 0.18155 0.00611 . . 39 97 ASN N 0.16124 0.00550 . . 40 98 GLY N 0.16102 0.00719 . . 41 100 ILE N 0.17873 0.00928 . . 42 102 ALA N 0.15140 0.01359 . . 43 106 ARG N 0.15498 0.00428 . . 44 109 MET N 0.15910 0.00452 . . 45 111 ASN N 0.15799 0.00629 . . 46 112 LEU N 0.15428 0.00468 . . 47 113 GLY N 0.16568 0.00895 . . 48 114 GLU N 0.17558 0.00795 . . 49 116 LEU N 0.25261 0.03008 . . 50 117 THR N 0.19063 0.01130 . . 51 120 GLU N 0.17169 0.00498 . . 52 123 GLU N 0.16143 0.00351 . . 53 125 ILE N 0.14690 0.00557 . . 54 127 GLU N 0.15199 0.00428 . . 55 128 ALA N 0.14601 0.00469 . . 56 129 ASP N 0.16902 0.00466 . . 57 130 ILE N 0.21547 0.00763 . . 58 132 GLY N 0.17713 0.00569 . . 59 133 ASP N 0.15507 0.00460 . . 60 134 GLY N 0.16786 0.00640 . . 61 135 GLN N 0.15880 0.00425 . . 62 136 VAL N 0.16048 0.00558 . . 63 137 ASN N 0.15893 0.00750 . . 64 138 TYR N 0.15797 0.00579 . . 65 142 VAL N 0.15806 0.00454 . . 66 143 GLN N 0.16545 0.00400 . . 67 144 MET N 0.15450 0.00478 . . 68 145 MET N 0.17420 0.00615 . . 69 146 THR N 0.16986 0.00880 . . 70 148 LYS N 0.40606 0.03027 . . stop_ save_ save_2H_T2_600MHz_Dy_15C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_6 _Spectrometer_frequency_1H 600 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.00953 0.00069 . . 2 9 ILE HD1 0.00876 0.00024 . . 3 10 ALA HB 0.00503 0.00024 . . 4 15 ALA HB 0.00647 0.00121 . . 5 18 LEU HD2 0.00883 0.00056 . . 6 26 THR HG2 0.00519 0.00036 . . 7 27 ILE HD1 0.00616 0.00076 . . 8 27 ILE HG2 0.00643 0.00047 . . 9 29 THR HG2 0.00618 0.00034 . . 10 34 THR HG2 0.00598 0.00026 . . 11 35 VAL HG1 0.00608 0.00037 . . 12 35 VAL HG2 0.00690 0.00032 . . 13 36 MET HE 0.01422 0.00012 . . 14 39 LEU HD1 0.00858 0.00098 . . 15 39 LEU HD2 0.00742 0.00044 . . 16 46 ALA HB 0.00566 0.00022 . . 17 51 MET HE 0.01506 0.00016 . . 18 52 ILE HD1 0.01233 0.00034 . . 19 52 ILE HG2 0.00543 0.00036 . . 20 55 VAL HG1 0.00768 0.00023 . . 21 55 VAL HG2 0.00829 0.00030 . . 22 63 ILE HD1 0.00608 0.00044 . . 23 63 ILE HG2 0.00599 0.00045 . . 24 69 LEU HD1 0.01082 0.00131 . . 25 70 THR HG2 0.00586 0.00024 . . 26 71 MET HE 0.00919 0.00021 . . 27 72 MET HE 0.00611 0.00043 . . 28 73 ALA HB 0.00588 0.00032 . . 29 76 MET HE 0.03249 0.00034 . . 30 79 THR HG2 0.00945 0.00019 . . 31 85 ILE HD1 0.01299 0.00031 . . 32 85 ILE HG2 0.00662 0.00033 . . 33 88 ALA HB 0.00702 0.00091 . . 34 91 VAL HG1 0.00530 0.00037 . . 35 100 ILE HD1 0.00558 0.00075 . . 36 102 ALA HB 0.00494 0.00030 . . 37 103 ALA HB 0.00514 0.00021 . . 38 105 LEU HD1 0.00731 0.00091 . . 39 108 VAL HG1 0.00769 0.00026 . . 40 108 VAL HG2 0.00861 0.00024 . . 41 109 MET HE 0.01175 0.00013 . . 42 110 THR HG2 0.00629 0.00020 . . 43 112 LEU HD2 0.00682 0.00057 . . 44 116 LEU HD1 0.01356 0.00061 . . 45 121 VAL HG1 0.00627 0.00025 . . 46 124 MET HE 0.00577 0.00069 . . 47 125 ILE HD1 0.00939 0.00031 . . 48 125 ILE HG2 0.00582 0.00031 . . 49 128 ALA HB 0.00491 0.00068 . . 50 130 ILE HD1 0.01192 0.00019 . . 51 130 ILE HG2 0.00711 0.00015 . . 52 136 VAL HG1 0.00547 0.00069 . . 53 142 VAL HG1 0.00592 0.00023 . . 54 142 VAL HG2 0.00698 0.00026 . . 55 144 MET HE 0.00998 0.00043 . . 56 145 MET HE 0.01542 0.00030 . . 57 146 THR HG2 0.00737 0.00017 . . 58 147 ALA HB 0.00935 0.00012 . . stop_ save_ save_2H_T2_600MHz_Dy_22C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 600 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.01155 0.00086 . . 2 9 ILE HD1 0.01044 0.00036 . . 3 10 ALA HB 0.00660 0.00037 . . 4 15 ALA HB 0.00815 0.00110 . . 5 18 LEU HD2 0.01137 0.00069 . . 6 26 THR HG2 0.00645 0.00045 . . 7 27 ILE HD1 0.00697 0.00083 . . 8 27 ILE HG2 0.00679 0.00059 . . 9 29 THR HG2 0.00778 0.00049 . . 10 34 THR HG2 0.00712 0.00036 . . 11 35 VAL HG1 0.00734 0.00047 . . 12 35 VAL HG2 0.00859 0.00041 . . 13 36 MET HE 0.01694 0.00019 . . 14 39 LEU HD1 0.00928 0.00107 . . 15 39 LEU HD2 0.00965 0.00068 . . 16 46 ALA HB 0.00607 0.00031 . . 17 51 MET HE 0.01844 0.00021 . . 18 52 ILE HD1 0.01504 0.00052 . . 19 52 ILE HG2 0.00700 0.00049 . . 20 55 VAL HG1 0.00938 0.00031 . . 21 55 VAL HG2 0.01010 0.00040 . . 22 63 ILE HD1 0.00723 0.00057 . . 23 63 ILE HG2 0.00713 0.00057 . . 24 69 LEU HD1 0.01369 0.00159 . . 25 70 THR HG2 0.00753 0.00035 . . 26 71 MET HE 0.01196 0.00025 . . 27 72 MET HE 0.00777 0.00042 . . 28 73 ALA HB 0.00648 0.00035 . . 29 76 MET HE 0.03924 0.00048 . . 30 79 THR HG2 0.01080 0.00025 . . 31 85 ILE HD1 0.01580 0.00046 . . 32 85 ILE HG2 0.00776 0.00041 . . 33 88 ALA HB 0.00592 0.00080 . . 34 91 VAL HG1 0.00700 0.00047 . . 35 100 ILE HD1 0.00618 0.00080 . . 36 102 ALA HB 0.00577 0.00040 . . 37 103 ALA HB 0.00575 0.00032 . . 38 105 LEU HD1 0.00833 0.00100 . . 39 108 VAL HG1 0.00986 0.00040 . . 40 108 VAL HG2 0.01154 0.00039 . . 41 109 MET HE 0.01449 0.00020 . . 42 110 THR HG2 0.00752 0.00031 . . 43 112 LEU HD2 0.00793 0.00073 . . 44 116 LEU HD1 0.01558 0.00084 . . 45 121 VAL HG1 0.00830 0.00038 . . 46 124 MET HE 0.00649 0.00045 . . 47 125 ILE HD1 0.01096 0.00042 . . 48 125 ILE HG2 0.00631 0.00040 . . 49 128 ALA HB 0.00531 0.00061 . . 50 130 ILE HD1 0.01388 0.00032 . . 51 130 ILE HG2 0.00866 0.00022 . . 52 136 VAL HG1 0.00619 0.00069 . . 53 142 VAL HG1 0.00728 0.00033 . . 54 142 VAL HG2 0.00913 0.00042 . . 55 144 MET HE 0.01273 0.00036 . . 56 145 MET HE 0.01738 0.00029 . . 57 146 THR HG2 0.00884 0.00027 . . 58 147 ALA HB 0.01158 0.00020 . . stop_ save_ save_2H_T2_600MHz_Dy_28C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_7 _Spectrometer_frequency_1H 600 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.01363 0.00067 . . 2 9 ILE HD1 0.01236 0.00026 . . 3 10 ALA HB 0.00715 0.00021 . . 4 15 ALA HB 0.00686 0.00054 . . 5 18 LEU HD2 0.01343 0.00056 . . 6 26 THR HG2 0.00766 0.00027 . . 7 27 ILE HD1 0.00796 0.00055 . . 8 27 ILE HG2 0.00902 0.00040 . . 9 29 THR HG2 0.00909 0.00030 . . 10 34 THR HG2 0.00841 0.00024 . . 11 35 VAL HG1 0.00831 0.00030 . . 12 35 VAL HG2 0.01011 0.00032 . . 13 36 MET HE 0.02073 0.00016 . . 14 39 LEU HD1 0.01064 0.00073 . . 15 39 LEU HD2 0.01112 0.00050 . . 16 46 ALA HB 0.00748 0.00020 . . 17 51 MET HE 0.02300 0.00019 . . 18 52 ILE HD1 0.01810 0.00041 . . 19 52 ILE HG2 0.00796 0.00029 . . 20 55 VAL HG1 0.01118 0.00024 . . 21 55 VAL HG2 0.01222 0.00030 . . 22 63 ILE HD1 0.00857 0.00035 . . 23 63 ILE HG2 0.00814 0.00035 . . 24 69 LEU HD1 0.01561 0.00138 . . 25 70 THR HG2 0.00863 0.00022 . . 26 71 MET HE 0.01479 0.00021 . . 27 72 MET HE 0.00968 0.00029 . . 28 73 ALA HB 0.00773 0.00027 . . 29 76 MET HE 0.04891 0.00054 . . 30 79 THR HG2 0.01240 0.00020 . . 31 85 ILE HD1 0.01773 0.00038 . . 32 85 ILE HG2 0.00967 0.00029 . . 33 88 ALA HB 0.00636 0.00040 . . 34 91 VAL HG1 0.00786 0.00031 . . 35 100 ILE HD1 0.00798 0.00053 . . 36 102 ALA HB 0.00669 0.00023 . . 37 103 ALA HB 0.00721 0.00021 . . 38 105 LEU HD1 0.01016 0.00069 . . 39 108 VAL HG1 0.01082 0.00026 . . 40 108 VAL HG2 0.01301 0.00029 . . 41 109 MET HE 0.01706 0.00016 . . 42 110 THR HG2 0.00903 0.00020 . . 43 112 LEU HD2 0.00969 0.00054 . . 44 116 LEU HD1 0.01937 0.00079 . . 45 121 VAL HG1 0.00907 0.00023 . . 46 124 MET HE 0.00791 0.00027 . . 47 125 ILE HD1 0.01357 0.00031 . . 48 125 ILE HG2 0.00756 0.00024 . . 49 128 ALA HB 0.00664 0.00043 . . 50 130 ILE HD1 0.01633 0.00025 . . 51 130 ILE HG2 0.00997 0.00015 . . 52 136 VAL HG1 0.00730 0.00042 . . 53 142 VAL HG1 0.00882 0.00021 . . 54 142 VAL HG2 0.01009 0.00025 . . 55 144 MET HE 0.01554 0.00028 . . 56 145 MET HE 0.02145 0.00028 . . 57 146 THR HG2 0.01044 0.00019 . . 58 147 ALA HB 0.01345 0.00015 . . stop_ save_ save_2H_T2_600MHz_Dy_35C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_2 _Spectrometer_frequency_1H 600 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.01745 0.00070 . . 2 9 ILE HD1 0.01497 0.00029 . . 3 10 ALA HB 0.00845 0.00019 . . 4 15 ALA HB 0.00797 0.00034 . . 5 18 LEU HD2 0.01731 0.00053 . . 6 26 THR HG2 0.00930 0.00023 . . 7 27 ILE HD1 0.00972 0.00047 . . 8 27 ILE HG2 0.00912 0.00030 . . 9 29 THR HG2 0.01110 0.00029 . . 10 34 THR HG2 0.01048 0.00024 . . 11 35 VAL HG1 0.00998 0.00025 . . 12 35 VAL HG2 0.01174 0.00026 . . 13 36 MET HE 0.02576 0.00021 . . 14 39 LEU HD1 0.01384 0.00076 . . 15 39 LEU HD2 0.01348 0.00047 . . 16 46 ALA HB 0.00876 0.00018 . . 17 51 MET HE 0.02794 0.00024 . . 18 52 ILE HD1 0.02217 0.00046 . . 19 52 ILE HG2 0.00942 0.00025 . . 20 55 VAL HG1 0.01315 0.00021 . . 21 55 VAL HG2 0.01448 0.00028 . . 22 63 ILE HD1 0.01036 0.00032 . . 23 63 ILE HG2 0.01009 0.00032 . . 24 69 LEU HD1 0.01728 0.00117 . . 25 70 THR HG2 0.01033 0.00021 . . 26 71 MET HE 0.01927 0.00022 . . 27 72 MET HE 0.01129 0.00021 . . 28 73 ALA HB 0.00928 0.00021 . . 29 76 MET HE 0.06136 0.00073 . . 30 79 THR HG2 0.01500 0.00020 . . 31 85 ILE HD1 0.02130 0.00041 . . 32 85 ILE HG2 0.01173 0.00027 . . 33 88 ALA HB 0.00750 0.00031 . . 34 91 VAL HG1 0.00940 0.00025 . . 35 100 ILE HD1 0.00888 0.00045 . . 36 102 ALA HB 0.00826 0.00023 . . 37 103 ALA HB 0.00846 0.00019 . . 38 105 LEU HD1 0.01266 0.00060 . . 39 108 VAL HG1 0.01292 0.00026 . . 40 108 VAL HG2 0.01537 0.00028 . . 41 109 MET HE 0.02074 0.00018 . . 42 110 THR HG2 0.01053 0.00020 . . 43 112 LEU HD2 0.01229 0.00052 . . 44 116 LEU HD1 0.02299 0.00087 . . 45 121 VAL HG1 0.01126 0.00022 . . 46 124 MET HE 0.00962 0.00022 . . 47 125 ILE HD1 0.01729 0.00034 . . 48 125 ILE HG2 0.00902 0.00023 . . 49 128 ALA HB 0.00720 0.00029 . . 50 130 ILE HD1 0.01931 0.00031 . . 51 130 ILE HG2 0.01242 0.00017 . . 52 136 VAL HG1 0.00841 0.00034 . . 53 142 VAL HG1 0.01023 0.00020 . . 54 142 VAL HG2 0.01272 0.00027 . . 55 144 MET HE 0.01873 0.00027 . . 56 145 MET HE 0.02502 0.00027 . . 57 146 THR HG2 0.01278 0.00021 . . 58 147 ALA HB 0.01637 0.00017 . . stop_ save_ save_2H_T2_600MHz_Dy_38C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_8 _Spectrometer_frequency_1H 600 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.02061 0.00079 . . 2 9 ILE HD1 0.01583 0.00028 . . 3 10 ALA HB 0.00946 0.00018 . . 4 15 ALA HB 0.00876 0.00042 . . 5 18 LEU HD2 0.01814 0.00055 . . 6 26 THR HG2 0.00976 0.00021 . . 7 27 ILE HD1 0.00959 0.00045 . . 8 27 ILE HG2 0.01107 0.00033 . . 9 29 THR HG2 0.01183 0.00026 . . 10 34 THR HG2 0.01116 0.00022 . . 11 35 VAL HG1 0.01060 0.00025 . . 12 35 VAL HG2 0.01249 0.00027 . . 13 39 LEU HD1 0.01422 0.00072 . . 14 39 LEU HD2 0.01431 0.00048 . . 15 46 ALA HB 0.00965 0.00018 . . 16 52 ILE HD1 0.02374 0.00044 . . 17 52 ILE HG2 0.01011 0.00024 . . 18 55 VAL HG1 0.01416 0.00021 . . 19 55 VAL HG2 0.01558 0.00029 . . 20 63 ILE HD1 0.01108 0.00034 . . 21 63 ILE HG2 0.01048 0.00030 . . 22 69 LEU HD1 0.01990 0.00142 . . 23 70 THR HG2 0.01186 0.00022 . . 24 71 MET HE 0.02181 0.00026 . . 25 72 MET HE 0.01267 0.00027 . . 26 73 ALA HB 0.00947 0.00022 . . 27 76 MET HE 0.06936 0.00096 . . 28 79 THR HG2 0.01712 0.00025 . . 29 85 ILE HD1 0.02280 0.00043 . . 30 85 ILE HG2 0.01217 0.00026 . . 31 88 ALA HB 0.00879 0.00033 . . 32 100 ILE HD1 0.00958 0.00043 . . 33 102 ALA HB 0.00876 0.00021 . . 34 103 ALA HB 0.00912 0.00019 . . 35 105 LEU HD1 0.01315 0.00055 . . 36 108 VAL HG1 0.01389 0.00026 . . 37 108 VAL HG2 0.01676 0.00026 . . 38 109 MET HE 0.02409 0.00021 . . 39 110 THR HG2 0.01158 0.00020 . . 40 112 LEU HD2 0.01304 0.00050 . . 41 116 LEU HD1 0.02496 0.00084 . . 42 121 VAL HG1 0.01189 0.00021 . . 43 124 MET HE 0.01064 0.00026 . . 44 125 ILE HD1 0.01928 0.00036 . . 45 125 ILE HG2 0.00972 0.00022 . . 46 128 ALA HB 0.00743 0.00028 . . 47 130 ILE HD1 0.02086 0.00027 . . 48 130 ILE HG2 0.01319 0.00016 . . 49 136 VAL HG1 0.00917 0.00033 . . 50 142 VAL HG2 0.01346 0.00023 . . 51 144 MET HE 0.02118 0.00033 . . 52 145 MET HE 0.02852 0.00033 . . 53 146 THR HG2 0.01296 0.00018 . . 54 147 ALA HB 0.01784 0.00017 . . stop_ save_ save_2H_T2_600MHz_Dy_41C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_9 _Spectrometer_frequency_1H 600 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.02084 0.00079 . . 2 9 ILE HD1 0.01728 0.00031 . . 3 10 ALA HB 0.00991 0.00020 . . 4 15 ALA HB 0.00958 0.00043 . . 5 18 LEU HD2 0.02120 0.00071 . . 6 26 THR HG2 0.01069 0.00023 . . 7 27 ILE HD1 0.01100 0.00053 . . 8 27 ILE HG2 0.01094 0.00030 . . 9 29 THR HG2 0.01267 0.00028 . . 10 34 THR HG2 0.01218 0.00024 . . 11 35 VAL HG1 0.01158 0.00028 . . 12 35 VAL HG2 0.01369 0.00030 . . 13 39 LEU HD1 0.01464 0.00075 . . 14 39 LEU HD2 0.01599 0.00054 . . 15 46 ALA HB 0.01012 0.00019 . . 16 52 ILE HD1 0.02557 0.00049 . . 17 52 ILE HG2 0.01089 0.00024 . . 18 55 VAL HG1 0.01519 0.00024 . . 19 55 VAL HG2 0.01658 0.00031 . . 20 63 ILE HD1 0.01185 0.00034 . . 21 63 ILE HG2 0.01157 0.00032 . . 22 69 LEU HD1 0.02247 0.00173 . . 23 70 THR HG2 0.01214 0.00023 . . 24 71 MET HE 0.02402 0.00030 . . 25 72 MET HE 0.01380 0.00027 . . 26 73 ALA HB 0.01066 0.00025 . . 27 76 MET HE 0.07028 0.00092 . . 28 79 THR HG2 0.01791 0.00026 . . 29 85 ILE HD1 0.02341 0.00045 . . 30 85 ILE HG2 0.01305 0.00029 . . 31 88 ALA HB 0.00925 0.00032 . . 32 100 ILE HD1 0.01048 0.00046 . . 33 102 ALA HB 0.00898 0.00021 . . 34 103 ALA HB 0.00980 0.00021 . . 35 105 LEU HD1 0.01388 0.00056 . . 36 108 VAL HG1 0.01431 0.00028 . . 37 108 VAL HG2 0.01797 0.00031 . . 38 109 MET HE 0.02504 0.00021 . . 39 110 THR HG2 0.01224 0.00022 . . 40 112 LEU HD2 0.01432 0.00059 . . 41 116 LEU HD1 0.02674 0.00098 . . 42 121 VAL HG1 0.01272 0.00022 . . 43 124 MET HE 0.01106 0.00024 . . 44 125 ILE HD1 0.02058 0.00039 . . 45 125 ILE HG2 0.01052 0.00024 . . 46 128 ALA HB 0.00858 0.00032 . . 47 130 ILE HD1 0.02232 0.00032 . . 48 130 ILE HG2 0.01409 0.00018 . . 49 136 VAL HG1 0.00946 0.00034 . . 50 142 VAL HG2 0.01407 0.00025 . . 51 144 MET HE 0.02274 0.00032 . . 52 145 MET HE 0.02911 0.00034 . . 53 146 THR HG2 0.01418 0.00021 . . 54 147 ALA HB 0.01883 0.00019 . . stop_ save_ save_2H_T2_600MHz_Dy_44C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_10 _Spectrometer_frequency_1H 600 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.02427 0.00084 . . 2 9 ILE HD1 0.01898 0.00029 . . 3 10 ALA HB 0.01063 0.00017 . . 4 15 ALA HB 0.01007 0.00036 . . 5 18 LEU HD2 0.02194 0.00060 . . 6 26 THR HG2 0.01168 0.00020 . . 7 27 ILE HD1 0.01185 0.00048 . . 8 27 ILE HG2 0.01183 0.00027 . . 9 29 THR HG2 0.01378 0.00024 . . 10 34 THR HG2 0.01337 0.00022 . . 11 35 VAL HG1 0.01221 0.00022 . . 12 35 VAL HG2 0.01433 0.00025 . . 13 39 LEU HD1 0.01643 0.00068 . . 14 39 LEU HD2 0.01700 0.00048 . . 15 46 ALA HB 0.01064 0.00016 . . 16 52 ILE HD1 0.02729 0.00047 . . 17 52 ILE HG2 0.01175 0.00022 . . 18 55 VAL HG1 0.01677 0.00023 . . 19 55 VAL HG2 0.01834 0.00029 . . 20 63 ILE HD1 0.01309 0.00032 . . 21 63 ILE HG2 0.01213 0.00028 . . 22 69 LEU HD1 0.02256 0.00139 . . 23 70 THR HG2 0.01354 0.00021 . . 24 71 MET HE 0.02627 0.00028 . . 25 72 MET HE 0.01507 0.00026 . . 26 73 ALA HB 0.01118 0.00023 . . 27 76 MET HE 0.07996 0.00128 . . 28 79 THR HG2 0.01945 0.00026 . . 29 85 ILE HD1 0.02562 0.00044 . . 30 85 ILE HG2 0.01375 0.00024 . . 31 88 ALA HB 0.00969 0.00026 . . 32 100 ILE HD1 0.01110 0.00039 . . 33 102 ALA HB 0.01015 0.00020 . . 34 103 ALA HB 0.01009 0.00017 . . 35 105 LEU HD1 0.01540 0.00053 . . 36 108 VAL HG1 0.01508 0.00024 . . 37 108 VAL HG2 0.01866 0.00025 . . 38 109 MET HE 0.02788 0.00022 . . 39 110 THR HG2 0.01306 0.00019 . . 40 112 LEU HD2 0.01585 0.00052 . . 41 116 LEU HD1 0.02951 0.00090 . . 42 121 VAL HG1 0.01355 0.00020 . . 43 124 MET HE 0.01218 0.00024 . . 44 125 ILE HD1 0.02245 0.00037 . . 45 125 ILE HG2 0.01130 0.00020 . . 46 128 ALA HB 0.00906 0.00027 . . 47 130 ILE HD1 0.02416 0.00032 . . 48 130 ILE HG2 0.01506 0.00016 . . 49 136 VAL HG1 0.01056 0.00030 . . 50 142 VAL HG2 0.01546 0.00023 . . 51 144 MET HE 0.02479 0.00038 . . 52 145 MET HE 0.03167 0.00035 . . 53 146 THR HG2 0.01462 0.00018 . . 54 147 ALA HB 0.02042 0.00018 . . stop_ save_ save_2H_T2_600MHz_Dy_47C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_3 _Spectrometer_frequency_1H 600 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.02487 0.00102 . . 2 9 ILE HD1 0.01973 0.00039 . . 3 10 ALA HB 0.01086 0.00021 . . 4 15 ALA HB 0.01053 0.00040 . . 5 18 LEU HD2 0.02363 0.00074 . . 6 26 THR HG2 0.01230 0.00027 . . 7 27 ILE HD1 0.01197 0.00052 . . 8 27 ILE HG2 0.01168 0.00032 . . 9 29 THR HG2 0.01469 0.00035 . . 10 34 THR HG2 0.01375 0.00028 . . 11 35 VAL HG1 0.01285 0.00029 . . 12 35 VAL HG2 0.01543 0.00034 . . 13 39 LEU HD1 0.01712 0.00083 . . 14 39 LEU HD2 0.01805 0.00060 . . 15 46 ALA HB 0.01122 0.00025 . . 16 52 ILE HD1 0.02972 0.00068 . . 17 52 ILE HG2 0.01229 0.00028 . . 18 55 VAL HG1 0.01762 0.00028 . . 19 55 VAL HG2 0.01921 0.00036 . . 20 63 ILE HD1 0.01351 0.00039 . . 21 63 ILE HG2 0.01257 0.00034 . . 22 69 LEU HD1 0.02353 0.00156 . . 23 70 THR HG2 0.01391 0.00026 . . 24 71 MET HE 0.02928 0.00035 . . 25 72 MET HE 0.01617 0.00029 . . 26 73 ALA HB 0.01150 0.00025 . . 27 76 MET HE 0.08977 0.00154 . . 28 79 THR HG2 0.02040 0.00028 . . 29 85 ILE HD1 0.02712 0.00059 . . 30 85 ILE HG2 0.01469 0.00031 . . 31 88 ALA HB 0.00969 0.00035 . . 32 100 ILE HD1 0.01151 0.00049 . . 33 102 ALA HB 0.01002 0.00025 . . 34 103 ALA HB 0.01098 0.00025 . . 35 105 LEU HD1 0.01617 0.00068 . . 36 108 VAL HG1 0.01582 0.00031 . . 37 108 VAL HG2 0.01882 0.00028 . . 38 109 MET HE 0.02971 0.00029 . . 39 110 THR HG2 0.01383 0.00025 . . 40 112 LEU HD2 0.01569 0.00059 . . 41 116 LEU HD1 0.02985 0.00114 . . 42 121 VAL HG1 0.01433 0.00027 . . 43 124 MET HE 0.01256 0.00023 . . 44 125 ILE HD1 0.02371 0.00046 . . 45 125 ILE HG2 0.01167 0.00026 . . 46 128 ALA HB 0.00930 0.00032 . . 47 130 ILE HD1 0.02598 0.00044 . . 48 130 ILE HG2 0.01573 0.00022 . . 49 136 VAL HG1 0.01086 0.00038 . . 50 142 VAL HG2 0.01604 0.00030 . . 51 144 MET HE 0.02577 0.00034 . . 52 145 MET HE 0.03306 0.00042 . . 53 146 THR HG2 0.01586 0.00024 . . 54 147 ALA HB 0.02144 0.00024 . . stop_ save_ save_2H_T2_600MHz_Dy_50C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_11 _Spectrometer_frequency_1H 600 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.02648 0.00123 . . 2 9 ILE HD1 0.02131 0.00047 . . 3 10 ALA HB 0.01148 0.00024 . . 4 15 ALA HB 0.01089 0.00048 . . 5 18 LEU HD2 0.02657 0.00103 . . 6 26 THR HG2 0.01303 0.00028 . . 7 27 ILE HD1 0.01267 0.00065 . . 8 27 ILE HG2 0.01359 0.00041 . . 9 29 THR HG2 0.01584 0.00036 . . 10 34 THR HG2 0.01458 0.00031 . . 11 35 VAL HG1 0.01362 0.00035 . . 12 35 VAL HG2 0.01600 0.00038 . . 13 39 LEU HD1 0.01932 0.00109 . . 14 39 LEU HD2 0.01933 0.00072 . . 15 46 ALA HB 0.01187 0.00025 . . 16 52 ILE HD1 0.03119 0.00075 . . 17 52 ILE HG2 0.01310 0.00031 . . 18 55 VAL HG1 0.01910 0.00036 . . 19 55 VAL HG2 0.02050 0.00045 . . 20 63 ILE HD1 0.01450 0.00044 . . 21 63 ILE HG2 0.01308 0.00038 . . 22 69 LEU HD1 0.02676 0.00244 . . 23 70 THR HG2 0.01542 0.00031 . . 24 71 MET HE 0.03215 0.00050 . . 25 72 MET HE 0.01749 0.00042 . . 26 73 ALA HB 0.01243 0.00033 . . 27 76 MET HE 0.09897 0.00273 . . 28 79 THR HG2 0.02170 0.00040 . . 29 85 ILE HD1 0.02890 0.00072 . . 30 85 ILE HG2 0.01573 0.00038 . . 31 88 ALA HB 0.01048 0.00035 . . 32 100 ILE HD1 0.01216 0.00057 . . 33 102 ALA HB 0.01108 0.00029 . . 34 103 ALA HB 0.01127 0.00025 . . 35 105 LEU HD1 0.01789 0.00083 . . 36 108 VAL HG1 0.01636 0.00035 . . 37 108 VAL HG2 0.01965 0.00030 . . 38 109 MET HE 0.03152 0.00038 . . 39 110 THR HG2 0.01452 0.00027 . . 40 112 LEU HD2 0.01699 0.00073 . . 41 116 LEU HD1 0.03059 0.00136 . . 42 121 VAL HG1 0.01501 0.00028 . . 43 124 MET HE 0.01326 0.00034 . . 44 125 ILE HD1 0.02610 0.00064 . . 45 125 ILE HG2 0.01253 0.00028 . . 46 128 ALA HB 0.01012 0.00037 . . 47 130 ILE HD1 0.02676 0.00051 . . 48 130 ILE HG2 0.01689 0.00025 . . 49 136 VAL HG1 0.01158 0.00041 . . 50 142 VAL HG2 0.01700 0.00036 . . 51 144 MET HE 0.02843 0.00064 . . 52 145 MET HE 0.03649 0.00061 . . 53 146 THR HG2 0.01660 0.00028 . . 54 147 ALA HB 0.02254 0.00028 . . stop_ save_ save_2H_T2_600MHz_Dy_53C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_12 _Spectrometer_frequency_1H 600 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.02934 0.00111 . . 2 9 ILE HD1 0.02225 0.00037 . . 3 10 ALA HB 0.01231 0.00019 . . 4 15 ALA HB 0.01123 0.00034 . . 5 18 LEU HD2 0.02734 0.00079 . . 6 26 THR HG2 0.01397 0.00023 . . 7 27 ILE HD1 0.01307 0.00046 . . 8 27 ILE HG2 0.01339 0.00028 . . 9 29 THR HG2 0.01650 0.00028 . . 10 34 THR HG2 0.01577 0.00025 . . 11 35 VAL HG1 0.01447 0.00027 . . 12 35 VAL HG2 0.01694 0.00029 . . 13 39 LEU HD1 0.01991 0.00088 . . 14 39 LEU HD2 0.02079 0.00062 . . 15 46 ALA HB 0.01275 0.00020 . . 16 52 ILE HD1 0.03281 0.00063 . . 17 52 ILE HG2 0.01383 0.00024 . . 18 55 VAL HG1 0.01967 0.00028 . . 19 55 VAL HG2 0.02162 0.00035 . . 20 63 ILE HD1 0.01533 0.00035 . . 21 63 ILE HG2 0.01370 0.00028 . . 22 69 LEU HD1 0.02773 0.00186 . . 23 70 THR HG2 0.01627 0.00024 . . 24 71 MET HE 0.03550 0.00041 . . 25 72 MET HE 0.01859 0.00030 . . 26 73 ALA HB 0.01306 0.00025 . . 27 76 MET HE 0.09746 0.00175 . . 28 79 THR HG2 0.02299 0.00032 . . 29 85 ILE HD1 0.02966 0.00056 . . 30 85 ILE HG2 0.01671 0.00028 . . 31 88 ALA HB 0.01088 0.00026 . . 32 100 ILE HD1 0.01304 0.00045 . . 33 102 ALA HB 0.01136 0.00021 . . 34 103 ALA HB 0.01213 0.00022 . . 35 105 LEU HD1 0.01876 0.00065 . . 36 108 VAL HG1 0.01751 0.00028 . . 37 109 MET HE 0.03402 0.00032 . . 38 110 THR HG2 0.01559 0.00023 . . 39 112 LEU HD2 0.01789 0.00056 . . 40 116 LEU HD1 0.03330 0.00118 . . 41 121 VAL HG1 0.01611 0.00023 . . 42 124 MET HE 0.01419 0.00024 . . 43 125 ILE HD1 0.02712 0.00047 . . 44 125 ILE HG2 0.01333 0.00023 . . 45 128 ALA HB 0.01047 0.00027 . . 46 130 ILE HD1 0.02854 0.00042 . . 47 130 ILE HG2 0.01761 0.00021 . . 48 136 VAL HG1 0.01226 0.00032 . . 49 142 VAL HG2 0.01802 0.00028 . . 50 144 MET HE 0.02948 0.00042 . . 51 145 MET HE 0.03771 0.00046 . . 52 146 THR HG2 0.01744 0.00022 . . 53 147 ALA HB 0.02379 0.00024 . . stop_ save_ save_2H_T2_600MHz_Dy_60C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_4 _Spectrometer_frequency_1H 600 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.03533 0.00202 . . 2 9 ILE HD1 0.02549 0.00053 . . 3 10 ALA HB 0.01367 0.00029 . . 4 15 ALA HB 0.01197 0.00044 . . 5 18 LEU HD2 0.03205 0.00109 . . 6 26 THR HG2 0.01607 0.00034 . . 7 27 ILE HD1 0.01433 0.00063 . . 8 27 ILE HG2 0.01502 0.00039 . . 9 29 THR HG2 0.01910 0.00041 . . 10 34 THR HG2 0.01757 0.00036 . . 11 35 VAL HG1 0.01583 0.00036 . . 12 35 VAL HG2 0.01863 0.00042 . . 13 39 LEU HD1 0.02218 0.00115 . . 14 39 LEU HD2 0.02238 0.00076 . . 15 46 ALA HB 0.01371 0.00029 . . 16 52 ILE HD1 0.03939 0.00098 . . 17 52 ILE HG2 0.01565 0.00033 . . 18 55 VAL HG1 0.02292 0.00042 . . 19 55 VAL HG2 0.02483 0.00050 . . 20 63 ILE HD1 0.01703 0.00048 . . 21 63 ILE HG2 0.01549 0.00043 . . 22 69 LEU HD1 0.03070 0.00240 . . 23 70 THR HG2 0.01862 0.00036 . . 24 71 MET HE 0.04646 0.00072 . . 25 72 MET HE 0.02209 0.00043 . . 26 73 ALA HB 0.01442 0.00034 . . 27 76 MET HE 0.11032 0.00258 . . 28 79 THR HG2 0.02677 0.00045 . . 29 85 ILE HD1 0.03295 0.00081 . . 30 85 ILE HG2 0.01832 0.00038 . . 31 88 ALA HB 0.01183 0.00038 . . 32 100 ILE HD1 0.01418 0.00058 . . 33 102 ALA HB 0.01233 0.00031 . . 34 103 ALA HB 0.01328 0.00030 . . 35 105 LEU HD1 0.02201 0.00089 . . 36 108 VAL HG1 0.01866 0.00038 . . 37 109 MET HE 0.04036 0.00049 . . 38 110 THR HG2 0.01721 0.00033 . . 39 112 LEU HD2 0.02125 0.00086 . . 40 116 LEU HD1 0.03851 0.00172 . . 41 121 VAL HG1 0.01805 0.00032 . . 42 124 MET HE 0.01579 0.00032 . . 43 125 ILE HD1 0.03119 0.00073 . . 44 125 ILE HG2 0.01471 0.00032 . . 45 128 ALA HB 0.01163 0.00038 . . 46 130 ILE HD1 0.03179 0.00062 . . 47 130 ILE HG2 0.02010 0.00030 . . 48 136 VAL HG1 0.01331 0.00042 . . 49 142 VAL HG2 0.02067 0.00042 . . 50 144 MET HE 0.03352 0.00055 . . 51 145 MET HE 0.04206 0.00068 . . 52 146 THR HG2 0.01961 0.00032 . . 53 147 ALA HB 0.02734 0.00038 . . stop_ save_ save_2H_T2_600MHz_Dy_67C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_13 _Spectrometer_frequency_1H 600 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.04120 0.00277 . . 2 9 ILE HD1 0.02889 0.00069 . . 3 10 ALA HB 0.01460 0.00032 . . 4 15 ALA HB 0.01297 0.00053 . . 5 18 LEU HD2 0.03629 0.00141 . . 6 26 THR HG2 0.01707 0.00032 . . 7 27 ILE HD1 0.01551 0.00073 . . 8 27 ILE HG2 0.01732 0.00044 . . 9 29 THR HG2 0.02088 0.00045 . . 10 34 THR HG2 0.01923 0.00040 . . 11 35 VAL HG1 0.01795 0.00045 . . 12 35 VAL HG2 0.02001 0.00048 . . 13 39 LEU HD1 0.02520 0.00138 . . 14 39 LEU HD2 0.02512 0.00092 . . 15 46 ALA HB 0.01504 0.00031 . . 16 52 ILE HD1 0.04266 0.00118 . . 17 52 ILE HG2 0.01698 0.00038 . . 18 55 VAL HG1 0.02497 0.00047 . . 19 55 VAL HG2 0.02879 0.00067 . . 20 63 ILE HD1 0.01949 0.00057 . . 21 63 ILE HG2 0.01647 0.00044 . . 22 69 LEU HD1 0.03502 0.00334 . . 23 70 THR HG2 0.02176 0.00038 . . 24 71 MET HE 0.05200 0.00096 . . 25 72 MET HE 0.02446 0.00069 . . 26 73 ALA HB 0.01511 0.00045 . . 27 76 MET HE 0.13136 0.00516 . . 28 79 THR HG2 0.02976 0.00063 . . 29 85 ILE HD1 0.03715 0.00112 . . 30 85 ILE HG2 0.02022 0.00046 . . 31 88 ALA HB 0.01262 0.00037 . . 32 100 ILE HD1 0.01570 0.00071 . . 33 102 ALA HB 0.01381 0.00034 . . 34 103 ALA HB 0.01442 0.00031 . . 35 105 LEU HD1 0.02450 0.00111 . . 36 108 VAL HG1 0.01990 0.00042 . . 37 109 MET HE 0.04473 0.00074 . . 38 110 THR HG2 0.01874 0.00036 . . 39 112 LEU HD2 0.02397 0.00103 . . 40 116 LEU HD1 0.03965 0.00191 . . 41 121 VAL HG1 0.02017 0.00038 . . 42 124 MET HE 0.01768 0.00055 . . 43 125 ILE HD1 0.03634 0.00100 . . 44 125 ILE HG2 0.01618 0.00035 . . 45 128 ALA HB 0.01270 0.00043 . . 46 130 ILE HD1 0.03508 0.00073 . . 47 130 ILE HG2 0.02191 0.00035 . . 48 136 VAL HG1 0.01471 0.00049 . . 49 142 VAL HG2 0.02194 0.00046 . . 50 144 MET HE 0.03897 0.00107 . . 51 145 MET HE 0.04761 0.00106 . . 52 146 THR HG2 0.02121 0.00036 . . 53 147 ALA HB 0.02932 0.00043 . . stop_ save_ save_2H_T2_600MHz_Dy_73C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_5 _Spectrometer_frequency_1H 600 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.04829 0.00567 . . 2 10 ALA HB 0.01563 0.00044 . . 3 15 ALA HB 0.01482 0.00102 . . 4 18 LEU HD2 0.03817 0.00253 . . 5 26 THR HG2 0.01872 0.00043 . . 6 27 ILE HD1 0.01800 0.00120 . . 7 27 ILE HG2 0.02032 0.00067 . . 8 29 THR HG2 0.02297 0.00059 . . 9 34 THR HG2 0.02095 0.00055 . . 10 35 VAL HG1 0.01920 0.00068 . . 11 35 VAL HG2 0.02219 0.00083 . . 12 39 LEU HD1 0.02655 0.00192 . . 13 39 LEU HD2 0.02950 0.00166 . . 14 46 ALA HB 0.01623 0.00044 . . 15 52 ILE HD1 0.04642 0.00176 . . 16 52 ILE HG2 0.01863 0.00054 . . 17 55 VAL HG1 0.02718 0.00069 . . 18 55 VAL HG2 0.03359 0.00113 . . 19 63 ILE HD1 0.02163 0.00081 . . 20 63 ILE HG2 0.01757 0.00063 . . 21 69 LEU HD1 0.03641 0.00510 . . 22 70 THR HG2 0.03179 0.00064 . . 23 71 MET HE 0.06684 0.00213 . . 24 72 MET HE 0.02845 0.00161 . . 25 73 ALA HB 0.01645 0.00083 . . 26 79 THR HG2 0.03535 0.00134 . . 27 85 ILE HD1 0.04037 0.00199 . . 28 85 ILE HG2 0.02126 0.00066 . . 29 88 ALA HB 0.01298 0.00049 . . 30 100 ILE HD1 0.01667 0.00107 . . 31 102 ALA HB 0.01462 0.00047 . . 32 103 ALA HB 0.01594 0.00044 . . 33 105 LEU HD1 0.02831 0.00198 . . 34 108 VAL HG1 0.02103 0.00064 . . 35 109 MET HE 0.04912 0.00126 . . 36 110 THR HG2 0.02018 0.00053 . . 37 112 LEU HD2 0.02509 0.00143 . . 38 116 LEU HD1 0.04809 0.00377 . . 39 121 VAL HG1 0.02157 0.00055 . . 40 124 MET HE 0.01895 0.00104 . . 41 125 ILE HD1 0.04098 0.00180 . . 42 125 ILE HG2 0.01768 0.00050 . . 43 128 ALA HB 0.01296 0.00068 . . 44 130 ILE HG2 0.02353 0.00051 . . 45 136 VAL HG1 0.01599 0.00074 . . 46 142 VAL HG2 0.02365 0.00064 . . 47 144 MET HE 0.04263 0.00221 . . 48 145 MET HE 0.04931 0.00185 . . 49 146 THR HG2 0.02243 0.00050 . . 50 147 ALA HB 0.03165 0.00061 . . stop_ save_ save_2H_T2_600MHz_Dz_15C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_6 _Spectrometer_frequency_1H 600 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.03427 0.00356 . . 2 9 ILE HD1 0.10609 0.00540 . . 3 10 ALA HB 0.04778 0.00323 . . 4 15 ALA HB 0.05400 0.01625 . . 5 18 LEU HD2 0.06423 0.00658 . . 6 26 THR HG2 0.02108 0.00210 . . 7 27 ILE HD1 0.07102 0.01629 . . 8 27 ILE HG2 0.05133 0.00570 . . 9 29 THR HG2 0.03239 0.00269 . . 10 34 THR HG2 0.03332 0.00202 . . 11 35 VAL HG1 0.03526 0.00308 . . 12 35 VAL HG2 0.07277 0.00544 . . 13 36 MET HE 0.15332 0.00291 . . 14 39 LEU HD1 0.04831 0.00870 . . 15 39 LEU HD2 0.06054 0.00588 . . 16 46 ALA HB 0.03740 0.00190 . . 17 51 MET HE 0.15176 0.00328 . . 18 52 ILE HD1 0.09696 0.00492 . . 19 52 ILE HG2 0.04642 0.00452 . . 20 55 VAL HG1 0.03105 0.00138 . . 21 55 VAL HG2 0.04025 0.00229 . . 22 63 ILE HD1 0.08894 0.01177 . . 23 63 ILE HG2 0.07614 0.01003 . . 24 69 LEU HD1 0.04585 0.00771 . . 25 70 THR HG2 0.04096 0.00231 . . 26 71 MET HE 0.16641 0.01057 . . 27 72 MET HE 0.20431 0.04333 . . 28 73 ALA HB 0.05240 0.00380 . . 29 76 MET HE 0.12557 0.00157 . . 30 79 THR HG2 0.04338 0.00124 . . 31 85 ILE HD1 0.07166 0.00253 . . 32 85 ILE HG2 0.05996 0.00463 . . 33 88 ALA HB 0.03090 0.00591 . . 34 91 VAL HG1 0.03987 0.00417 . . 35 100 ILE HD1 0.06927 0.01560 . . 36 102 ALA HB 0.05103 0.00378 . . 37 103 ALA HB 0.03744 0.00204 . . 38 105 LEU HD1 0.05915 0.01179 . . 39 108 VAL HG1 0.03570 0.00182 . . 40 108 VAL HG2 0.06179 0.00276 . . 41 109 MET HE 0.11194 0.00253 . . 42 110 THR HG2 0.03891 0.00170 . . 43 112 LEU HD2 0.06953 0.01024 . . 44 116 LEU HD1 0.03066 0.00190 . . 45 121 VAL HG1 0.04687 0.00279 . . 46 124 MET HE 0.11952 0.02551 . . 47 125 ILE HD1 0.07711 0.00440 . . 48 125 ILE HG2 0.04570 0.00341 . . 49 128 ALA HB 0.02803 0.00549 . . 50 130 ILE HD1 0.07921 0.00196 . . 51 130 ILE HG2 0.05277 0.00151 . . 52 136 VAL HG1 0.03750 0.00655 . . 53 142 VAL HG1 0.02785 0.00152 . . 54 142 VAL HG2 0.07767 0.00464 . . 55 144 MET HE 0.10630 0.00845 . . 56 145 MET HE 0.14085 0.00535 . . 57 146 THR HG2 0.03558 0.00115 . . 58 147 ALA HB 0.03926 0.00067 . . stop_ save_ save_2H_T2_600MHz_Dz_22C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 600 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.03999 0.00312 . . 2 9 ILE HD1 0.10487 0.00474 . . 3 10 ALA HB 0.04774 0.00260 . . 4 15 ALA HB 0.03562 0.00531 . . 5 18 LEU HD2 0.06219 0.00407 . . 6 26 THR HG2 0.02256 0.00183 . . 7 27 ILE HD1 0.07005 0.01008 . . 8 27 ILE HG2 0.05416 0.00509 . . 9 29 THR HG2 0.03397 0.00232 . . 10 34 THR HG2 0.03531 0.00183 . . 11 35 VAL HG1 0.03912 0.00270 . . 12 35 VAL HG2 0.07349 0.00405 . . 13 36 MET HE 0.15735 0.00294 . . 14 39 LEU HD1 0.04971 0.00663 . . 15 39 LEU HD2 0.06638 0.00527 . . 16 46 ALA HB 0.04377 0.00218 . . 17 51 MET HE 0.15094 0.00259 . . 18 52 ILE HD1 0.09816 0.00433 . . 19 52 ILE HG2 0.04790 0.00346 . . 20 55 VAL HG1 0.03503 0.00128 . . 21 55 VAL HG2 0.04001 0.00179 . . 22 63 ILE HD1 0.09090 0.00895 . . 23 63 ILE HG2 0.06670 0.00609 . . 24 69 LEU HD1 0.04985 0.00673 . . 25 70 THR HG2 0.04062 0.00183 . . 26 71 MET HE 0.14410 0.00512 . . 27 72 MET HE 0.15839 0.01390 . . 28 73 ALA HB 0.05246 0.00273 . . 29 76 MET HE 0.13645 0.00158 . . 30 79 THR HG2 0.04046 0.00097 . . 31 85 ILE HD1 0.07606 0.00244 . . 32 85 ILE HG2 0.06606 0.00404 . . 33 88 ALA HB 0.02387 0.00402 . . 34 91 VAL HG1 0.03891 0.00265 . . 35 100 ILE HD1 0.07853 0.01345 . . 36 102 ALA HB 0.05179 0.00350 . . 37 103 ALA HB 0.04025 0.00201 . . 38 105 LEU HD1 0.06207 0.00850 . . 39 108 VAL HG1 0.04176 0.00188 . . 40 108 VAL HG2 0.06704 0.00256 . . 41 109 MET HE 0.10625 0.00189 . . 42 110 THR HG2 0.04118 0.00161 . . 43 112 LEU HD2 0.07425 0.00835 . . 44 116 LEU HD1 0.03594 0.00212 . . 45 121 VAL HG1 0.04865 0.00238 . . 46 124 MET HE 0.14390 0.01540 . . 47 125 ILE HD1 0.08046 0.00383 . . 48 125 ILE HG2 0.05634 0.00379 . . 49 128 ALA HB 0.03131 0.00409 . . 50 130 ILE HD1 0.07856 0.00196 . . 51 130 ILE HG2 0.05590 0.00148 . . 52 136 VAL HG1 0.03795 0.00476 . . 53 142 VAL HG1 0.03096 0.00149 . . 54 142 VAL HG2 0.08315 0.00457 . . 55 144 MET HE 0.10079 0.00359 . . 56 145 MET HE 0.13505 0.00329 . . 57 146 THR HG2 0.03863 0.00120 . . 58 147 ALA HB 0.04162 0.00070 . . stop_ save_ save_2H_T2_600MHz_Dz_28C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_7 _Spectrometer_frequency_1H 600 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.04536 0.00371 . . 2 9 ILE HD1 0.10472 0.00454 . . 3 10 ALA HB 0.04986 0.00243 . . 4 15 ALA HB 0.04215 0.00547 . . 5 18 LEU HD2 0.06342 0.00451 . . 6 26 THR HG2 0.02412 0.00173 . . 7 27 ILE HD1 0.06328 0.00859 . . 8 27 ILE HG2 0.05904 0.00470 . . 9 29 THR HG2 0.03271 0.00188 . . 10 34 THR HG2 0.03879 0.00188 . . 11 35 VAL HG1 0.04016 0.00263 . . 12 35 VAL HG2 0.07038 0.00388 . . 13 36 MET HE 0.15833 0.00281 . . 14 39 LEU HD1 0.05020 0.00650 . . 15 39 LEU HD2 0.06858 0.00580 . . 16 46 ALA HB 0.04405 0.00194 . . 17 51 MET HE 0.15644 0.00268 . . 18 52 ILE HD1 0.10579 0.00472 . . 19 52 ILE HG2 0.04906 0.00308 . . 20 55 VAL HG1 0.03555 0.00133 . . 21 55 VAL HG2 0.03818 0.00159 . . 22 63 ILE HD1 0.09403 0.00823 . . 23 63 ILE HG2 0.07253 0.00587 . . 24 69 LEU HD1 0.04895 0.00762 . . 25 70 THR HG2 0.04469 0.00191 . . 26 71 MET HE 0.14466 0.00506 . . 27 72 MET HE 0.15294 0.01191 . . 28 73 ALA HB 0.05128 0.00293 . . 29 76 MET HE 0.15078 0.00206 . . 30 79 THR HG2 0.04312 0.00115 . . 31 85 ILE HD1 0.07594 0.00266 . . 32 85 ILE HG2 0.06690 0.00383 . . 33 88 ALA HB 0.02162 0.00287 . . 34 91 VAL HG1 0.03977 0.00268 . . 35 100 ILE HD1 0.06247 0.00823 . . 36 102 ALA HB 0.05100 0.00294 . . 37 103 ALA HB 0.04451 0.00222 . . 38 105 LEU HD1 0.05691 0.00705 . . 39 108 VAL HG1 0.04135 0.00176 . . 40 108 VAL HG2 0.06626 0.00259 . . 41 109 MET HE 0.10561 0.00177 . . 42 110 THR HG2 0.04630 0.00169 . . 43 112 LEU HD2 0.05816 0.00560 . . 44 116 LEU HD1 0.03699 0.00229 . . 45 121 VAL HG1 0.05145 0.00227 . . 46 124 MET HE 0.12656 0.00975 . . 47 125 ILE HD1 0.08031 0.00352 . . 48 125 ILE HG2 0.05151 0.00271 . . 49 128 ALA HB 0.02767 0.00329 . . 50 130 ILE HD1 0.07776 0.00197 . . 51 130 ILE HG2 0.05768 0.00143 . . 52 136 VAL HG1 0.03135 0.00310 . . 53 142 VAL HG1 0.03533 0.00158 . . 54 142 VAL HG2 0.07673 0.00351 . . 55 144 MET HE 0.09378 0.00318 . . 56 145 MET HE 0.13732 0.00362 . . 57 146 THR HG2 0.04321 0.00130 . . 58 147 ALA HB 0.04501 0.00084 . . stop_ save_ save_2H_T2_600MHz_Dz_35C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_2 _Spectrometer_frequency_1H 600 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.05100 0.00269 . . 2 9 ILE HD1 0.10264 0.00318 . . 3 10 ALA HB 0.05063 0.00153 . . 4 15 ALA HB 0.04458 0.00292 . . 5 18 LEU HD2 0.07133 0.00294 . . 6 26 THR HG2 0.02613 0.00103 . . 7 27 ILE HD1 0.06300 0.00491 . . 8 27 ILE HG2 0.05904 0.00296 . . 9 29 THR HG2 0.03712 0.00141 . . 10 34 THR HG2 0.04152 0.00126 . . 11 35 VAL HG1 0.04405 0.00169 . . 12 35 VAL HG2 0.07174 0.00238 . . 13 36 MET HE 0.16263 0.00233 . . 14 39 LEU HD1 0.04942 0.00381 . . 15 39 LEU HD2 0.07599 0.00393 . . 16 46 ALA HB 0.04698 0.00134 . . 17 51 MET HE 0.15392 0.00204 . . 18 52 ILE HD1 0.10812 0.00334 . . 19 52 ILE HG2 0.05032 0.00201 . . 20 55 VAL HG1 0.03754 0.00089 . . 21 55 VAL HG2 0.04347 0.00117 . . 22 63 ILE HD1 0.08743 0.00448 . . 23 63 ILE HG2 0.07645 0.00398 . . 24 69 LEU HD1 0.04802 0.00433 . . 25 70 THR HG2 0.04590 0.00127 . . 26 71 MET HE 0.13471 0.00268 . . 27 72 MET HE 0.13839 0.00550 . . 28 73 ALA HB 0.05394 0.00168 . . 29 76 MET HE 0.16465 0.00177 . . 30 79 THR HG2 0.04327 0.00075 . . 31 85 ILE HD1 0.07967 0.00197 . . 32 85 ILE HG2 0.07003 0.00248 . . 33 88 ALA HB 0.02801 0.00187 . . 34 91 VAL HG1 0.04245 0.00160 . . 35 100 ILE HD1 0.06999 0.00593 . . 36 102 ALA HB 0.05566 0.00220 . . 37 103 ALA HB 0.04831 0.00153 . . 38 105 LEU HD1 0.05790 0.00399 . . 39 108 VAL HG1 0.04647 0.00132 . . 40 108 VAL HG2 0.06991 0.00177 . . 41 109 MET HE 0.10316 0.00125 . . 42 110 THR HG2 0.04849 0.00124 . . 43 112 LEU HD2 0.06803 0.00423 . . 44 116 LEU HD1 0.04484 0.00198 . . 45 121 VAL HG1 0.05400 0.00153 . . 46 124 MET HE 0.11397 0.00480 . . 47 125 ILE HD1 0.08564 0.00248 . . 48 125 ILE HG2 0.05415 0.00187 . . 49 128 ALA HB 0.03163 0.00200 . . 50 130 ILE HD1 0.08172 0.00172 . . 51 130 ILE HG2 0.05923 0.00114 . . 52 136 VAL HG1 0.03935 0.00240 . . 53 142 VAL HG1 0.03705 0.00104 . . 54 142 VAL HG2 0.08194 0.00269 . . 55 144 MET HE 0.09319 0.00185 . . 56 145 MET HE 0.13828 0.00235 . . 57 146 THR HG2 0.04380 0.00098 . . 58 147 ALA HB 0.04846 0.00061 . . stop_ save_ save_2H_T2_600MHz_Dz_38C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_8 _Spectrometer_frequency_1H 600 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.05182 0.00184 . . 2 9 ILE HD1 0.10787 0.00262 . . 3 10 ALA HB 0.05370 0.00104 . . 4 15 ALA HB 0.04426 0.00227 . . 5 18 LEU HD2 0.06847 0.00215 . . 6 26 THR HG2 0.02708 0.00065 . . 7 27 ILE HD1 0.06318 0.00363 . . 8 27 ILE HG2 0.06026 0.00197 . . 9 29 THR HG2 0.03753 0.00082 . . 10 34 THR HG2 0.04405 0.00087 . . 11 35 VAL HG1 0.04366 0.00112 . . 12 35 VAL HG2 0.07403 0.00180 . . 13 39 LEU HD1 0.05113 0.00259 . . 14 39 LEU HD2 0.07536 0.00293 . . 15 46 ALA HB 0.04872 0.00091 . . 16 52 ILE HD1 0.11187 0.00263 . . 17 52 ILE HG2 0.05574 0.00142 . . 18 55 VAL HG1 0.04037 0.00067 . . 19 55 VAL HG2 0.04425 0.00087 . . 20 63 ILE HD1 0.08208 0.00312 . . 21 63 ILE HG2 0.07466 0.00252 . . 22 69 LEU HD1 0.04975 0.00338 . . 23 70 THR HG2 0.04612 0.00082 . . 24 71 MET HE 0.13700 0.00259 . . 25 72 MET HE 0.14459 0.00526 . . 26 73 ALA HB 0.05455 0.00138 . . 27 76 MET HE 0.17328 0.00224 . . 28 79 THR HG2 0.04551 0.00064 . . 29 85 ILE HD1 0.07938 0.00158 . . 30 85 ILE HG2 0.06935 0.00169 . . 31 88 ALA HB 0.03354 0.00135 . . 32 100 ILE HD1 0.06730 0.00360 . . 33 102 ALA HB 0.05399 0.00126 . . 34 103 ALA HB 0.04834 0.00100 . . 35 105 LEU HD1 0.06070 0.00279 . . 36 108 VAL HG1 0.04797 0.00092 . . 37 108 VAL HG2 0.07370 0.00135 . . 38 109 MET HE 0.10660 0.00109 . . 39 110 THR HG2 0.04960 0.00086 . . 40 112 LEU HD2 0.07170 0.00320 . . 41 116 LEU HD1 0.04528 0.00134 . . 42 121 VAL HG1 0.05464 0.00106 . . 43 124 MET HE 0.10847 0.00356 . . 44 125 ILE HD1 0.08927 0.00198 . . 45 125 ILE HG2 0.05919 0.00136 . . 46 128 ALA HB 0.03151 0.00128 . . 47 130 ILE HD1 0.08192 0.00117 . . 48 130 ILE HG2 0.05776 0.00074 . . 49 136 VAL HG1 0.04121 0.00160 . . 50 142 VAL HG2 0.07796 0.00159 . . 51 144 MET HE 0.09162 0.00176 . . 52 145 MET HE 0.13921 0.00224 . . 53 146 THR HG2 0.04405 0.00062 . . 54 147 ALA HB 0.05091 0.00047 . . stop_ save_ save_2H_T2_600MHz_Dz_41C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_9 _Spectrometer_frequency_1H 600 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.05395 0.00241 . . 2 9 ILE HD1 0.10309 0.00278 . . 3 10 ALA HB 0.05280 0.00128 . . 4 15 ALA HB 0.04363 0.00253 . . 5 18 LEU HD2 0.07223 0.00294 . . 6 26 THR HG2 0.02889 0.00087 . . 7 27 ILE HD1 0.06014 0.00378 . . 8 27 ILE HG2 0.05882 0.00214 . . 9 29 THR HG2 0.03779 0.00105 . . 10 34 THR HG2 0.04458 0.00108 . . 11 35 VAL HG1 0.04496 0.00138 . . 12 35 VAL HG2 0.07313 0.00218 . . 13 39 LEU HD1 0.05563 0.00369 . . 14 39 LEU HD2 0.07579 0.00340 . . 15 46 ALA HB 0.05029 0.00116 . . 16 52 ILE HD1 0.11419 0.00312 . . 17 52 ILE HG2 0.05120 0.00147 . . 18 55 VAL HG1 0.04018 0.00081 . . 19 55 VAL HG2 0.04596 0.00111 . . 20 63 ILE HD1 0.08388 0.00360 . . 21 63 ILE HG2 0.07213 0.00269 . . 22 69 LEU HD1 0.05577 0.00507 . . 23 70 THR HG2 0.04792 0.00111 . . 24 71 MET HE 0.13012 0.00253 . . 25 72 MET HE 0.13208 0.00467 . . 26 73 ALA HB 0.05501 0.00154 . . 27 76 MET HE 0.18071 0.00236 . . 28 79 THR HG2 0.04456 0.00073 . . 29 85 ILE HD1 0.08060 0.00192 . . 30 85 ILE HG2 0.07089 0.00217 . . 31 88 ALA HB 0.03323 0.00153 . . 32 100 ILE HD1 0.06904 0.00429 . . 33 102 ALA HB 0.05560 0.00162 . . 34 103 ALA HB 0.04878 0.00123 . . 35 105 LEU HD1 0.06488 0.00360 . . 36 108 VAL HG1 0.04840 0.00118 . . 37 108 VAL HG2 0.06974 0.00150 . . 38 109 MET HE 0.10292 0.00119 . . 39 110 THR HG2 0.05198 0.00112 . . 40 112 LEU HD2 0.06478 0.00343 . . 41 116 LEU HD1 0.04881 0.00193 . . 42 121 VAL HG1 0.05400 0.00123 . . 43 124 MET HE 0.10351 0.00342 . . 44 125 ILE HD1 0.08751 0.00222 . . 45 125 ILE HG2 0.05653 0.00156 . . 46 128 ALA HB 0.03386 0.00162 . . 47 130 ILE HD1 0.08121 0.00144 . . 48 130 ILE HG2 0.05869 0.00097 . . 49 136 VAL HG1 0.04220 0.00198 . . 50 142 VAL HG2 0.07790 0.00196 . . 51 144 MET HE 0.08801 0.00161 . . 52 145 MET HE 0.13591 0.00237 . . 53 146 THR HG2 0.04572 0.00085 . . 54 147 ALA HB 0.05178 0.00062 . . stop_ save_ save_2H_T2_600MHz_Dz_44C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_10 _Spectrometer_frequency_1H 600 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.06329 0.00216 . . 2 9 ILE HD1 0.10256 0.00214 . . 3 10 ALA HB 0.05471 0.00097 . . 4 15 ALA HB 0.04483 0.00180 . . 5 18 LEU HD2 0.07602 0.00232 . . 6 26 THR HG2 0.03072 0.00062 . . 7 27 ILE HD1 0.06155 0.00302 . . 8 27 ILE HG2 0.05835 0.00157 . . 9 29 THR HG2 0.03975 0.00075 . . 10 34 THR HG2 0.04523 0.00076 . . 11 35 VAL HG1 0.04629 0.00099 . . 12 35 VAL HG2 0.07275 0.00158 . . 13 39 LEU HD1 0.05395 0.00246 . . 14 39 LEU HD2 0.07565 0.00250 . . 15 46 ALA HB 0.05197 0.00088 . . 16 52 ILE HD1 0.11328 0.00256 . . 17 52 ILE HG2 0.05347 0.00111 . . 18 55 VAL HG1 0.04056 0.00059 . . 19 55 VAL HG2 0.04636 0.00081 . . 20 63 ILE HD1 0.08631 0.00290 . . 21 63 ILE HG2 0.07346 0.00212 . . 22 69 LEU HD1 0.05492 0.00348 . . 23 70 THR HG2 0.04859 0.00078 . . 24 71 MET HE 0.14397 0.00259 . . 25 72 MET HE 0.14098 0.00459 . . 26 73 ALA HB 0.05438 0.00122 . . 27 76 MET HE 0.18520 0.00286 . . 28 79 THR HG2 0.04719 0.00063 . . 29 85 ILE HD1 0.08356 0.00161 . . 30 85 ILE HG2 0.07430 0.00166 . . 31 88 ALA HB 0.03437 0.00113 . . 32 100 ILE HD1 0.06486 0.00286 . . 33 102 ALA HB 0.05531 0.00116 . . 34 103 ALA HB 0.04790 0.00086 . . 35 105 LEU HD1 0.06026 0.00231 . . 36 108 VAL HG1 0.04920 0.00086 . . 37 108 VAL HG2 0.07285 0.00118 . . 38 109 MET HE 0.11133 0.00118 . . 39 110 THR HG2 0.05119 0.00079 . . 40 112 LEU HD2 0.07052 0.00274 . . 41 116 LEU HD1 0.04893 0.00129 . . 42 121 VAL HG1 0.05638 0.00094 . . 43 124 MET HE 0.10278 0.00291 . . 44 125 ILE HD1 0.09072 0.00184 . . 45 125 ILE HG2 0.06000 0.00119 . . 46 128 ALA HB 0.03505 0.00116 . . 47 130 ILE HD1 0.08419 0.00122 . . 48 130 ILE HG2 0.05945 0.00070 . . 49 136 VAL HG1 0.04324 0.00138 . . 50 142 VAL HG2 0.07613 0.00142 . . 51 144 MET HE 0.08573 0.00149 . . 52 145 MET HE 0.14245 0.00238 . . 53 146 THR HG2 0.04608 0.00061 . . 54 147 ALA HB 0.05381 0.00048 . . stop_ save_ save_2H_T2_600MHz_Dz_47C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_3 _Spectrometer_frequency_1H 600 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.05674 0.00272 . . 2 9 ILE HD1 0.10551 0.00347 . . 3 10 ALA HB 0.05604 0.00152 . . 4 15 ALA HB 0.04281 0.00223 . . 5 18 LEU HD2 0.07654 0.00312 . . 6 26 THR HG2 0.03107 0.00097 . . 7 27 ILE HD1 0.06144 0.00419 . . 8 27 ILE HG2 0.06047 0.00245 . . 9 29 THR HG2 0.04205 0.00139 . . 10 34 THR HG2 0.04656 0.00125 . . 11 35 VAL HG1 0.04653 0.00147 . . 12 35 VAL HG2 0.07317 0.00234 . . 13 39 LEU HD1 0.05437 0.00351 . . 14 39 LEU HD2 0.07639 0.00360 . . 15 46 ALA HB 0.05225 0.00155 . . 16 52 ILE HD1 0.11709 0.00412 . . 17 52 ILE HG2 0.05595 0.00183 . . 18 55 VAL HG1 0.04247 0.00092 . . 19 55 VAL HG2 0.04761 0.00120 . . 20 63 ILE HD1 0.08461 0.00401 . . 21 63 ILE HG2 0.07336 0.00309 . . 22 69 LEU HD1 0.05648 0.00475 . . 23 70 THR HG2 0.04896 0.00124 . . 24 71 MET HE 0.13859 0.00289 . . 25 72 MET HE 0.13348 0.00477 . . 26 73 ALA HB 0.05778 0.00173 . . 27 76 MET HE 0.20173 0.00362 . . 28 79 THR HG2 0.04620 0.00079 . . 29 85 ILE HD1 0.08509 0.00242 . . 30 85 ILE HG2 0.07432 0.00243 . . 31 88 ALA HB 0.03369 0.00186 . . 32 100 ILE HD1 0.06948 0.00487 . . 33 102 ALA HB 0.05540 0.00180 . . 34 103 ALA HB 0.05147 0.00159 . . 35 105 LEU HD1 0.06392 0.00375 . . 36 108 VAL HG1 0.05164 0.00140 . . 37 108 VAL HG2 0.06994 0.00145 . . 38 109 MET HE 0.10977 0.00159 . . 39 110 THR HG2 0.05324 0.00128 . . 40 112 LEU HD2 0.07689 0.00447 . . 41 116 LEU HD1 0.05235 0.00222 . . 42 121 VAL HG1 0.05779 0.00157 . . 43 124 MET HE 0.10270 0.00339 . . 44 125 ILE HD1 0.09558 0.00287 . . 45 125 ILE HG2 0.05882 0.00183 . . 46 128 ALA HB 0.03686 0.00180 . . 47 130 ILE HD1 0.08488 0.00191 . . 48 130 ILE HG2 0.06020 0.00117 . . 49 136 VAL HG1 0.04485 0.00224 . . 50 142 VAL HG2 0.07911 0.00235 . . 51 144 MET HE 0.08665 0.00158 . . 52 145 MET HE 0.14011 0.00291 . . 53 146 THR HG2 0.04660 0.00094 . . 54 147 ALA HB 0.05467 0.00076 . . stop_ save_ save_2H_T2_600MHz_Dz_50C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_11 _Spectrometer_frequency_1H 600 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.06306 0.00245 . . 2 9 ILE HD1 0.10409 0.00252 . . 3 10 ALA HB 0.05486 0.00108 . . 4 15 ALA HB 0.04324 0.00188 . . 5 18 LEU HD2 0.07616 0.00250 . . 6 26 THR HG2 0.03226 0.00070 . . 7 27 ILE HD1 0.06591 0.00391 . . 8 27 ILE HG2 0.06108 0.00184 . . 9 29 THR HG2 0.04190 0.00092 . . 10 34 THR HG2 0.04741 0.00092 . . 11 35 VAL HG1 0.04869 0.00124 . . 12 35 VAL HG2 0.07152 0.00172 . . 13 39 LEU HD1 0.05641 0.00296 . . 14 39 LEU HD2 0.08148 0.00307 . . 15 46 ALA HB 0.05151 0.00101 . . 16 52 ILE HD1 0.11778 0.00278 . . 17 52 ILE HG2 0.05536 0.00128 . . 18 55 VAL HG1 0.04323 0.00075 . . 19 55 VAL HG2 0.04958 0.00100 . . 20 63 ILE HD1 0.08109 0.00275 . . 21 63 ILE HG2 0.07368 0.00236 . . 22 69 LEU HD1 0.05279 0.00394 . . 23 70 THR HG2 0.05040 0.00093 . . 24 71 MET HE 0.14866 0.00268 . . 25 72 MET HE 0.12968 0.00397 . . 26 73 ALA HB 0.05754 0.00150 . . 27 76 MET HE 0.20604 0.00345 . . 28 79 THR HG2 0.04848 0.00079 . . 29 85 ILE HD1 0.08640 0.00188 . . 30 85 ILE HG2 0.07072 0.00173 . . 31 88 ALA HB 0.03447 0.00120 . . 32 100 ILE HD1 0.07197 0.00383 . . 33 102 ALA HB 0.05744 0.00143 . . 34 103 ALA HB 0.05009 0.00103 . . 35 105 LEU HD1 0.06634 0.00301 . . 36 108 VAL HG1 0.05156 0.00110 . . 37 108 VAL HG2 0.06999 0.00106 . . 38 109 MET HE 0.11265 0.00125 . . 39 110 THR HG2 0.05389 0.00095 . . 40 112 LEU HD2 0.07758 0.00351 . . 41 116 LEU HD1 0.05560 0.00193 . . 42 121 VAL HG1 0.05738 0.00106 . . 43 124 MET HE 0.09547 0.00286 . . 44 125 ILE HD1 0.09623 0.00231 . . 45 125 ILE HG2 0.06107 0.00138 . . 46 128 ALA HB 0.03690 0.00135 . . 47 130 ILE HD1 0.08571 0.00142 . . 48 130 ILE HG2 0.05909 0.00082 . . 49 136 VAL HG1 0.04653 0.00172 . . 50 142 VAL HG2 0.07906 0.00172 . . 51 144 MET HE 0.08742 0.00170 . . 52 145 MET HE 0.14308 0.00240 . . 53 146 THR HG2 0.04793 0.00075 . . 54 147 ALA HB 0.05625 0.00059 . . stop_ save_ save_2H_T2_600MHz_Dz_53C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_12 _Spectrometer_frequency_1H 600 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.06723 0.00270 . . 2 9 ILE HD1 0.10318 0.00223 . . 3 10 ALA HB 0.05593 0.00110 . . 4 15 ALA HB 0.04172 0.00180 . . 5 18 LEU HD2 0.08051 0.00256 . . 6 26 THR HG2 0.03287 0.00079 . . 7 27 ILE HD1 0.05989 0.00300 . . 8 27 ILE HG2 0.06083 0.00173 . . 9 29 THR HG2 0.04310 0.00099 . . 10 34 THR HG2 0.05043 0.00101 . . 11 35 VAL HG1 0.04871 0.00123 . . 12 35 VAL HG2 0.07150 0.00162 . . 13 39 LEU HD1 0.06112 0.00347 . . 14 39 LEU HD2 0.08328 0.00310 . . 15 46 ALA HB 0.05368 0.00114 . . 16 52 ILE HD1 0.11986 0.00267 . . 17 52 ILE HG2 0.05779 0.00132 . . 18 55 VAL HG1 0.04489 0.00079 . . 19 55 VAL HG2 0.05071 0.00104 . . 20 63 ILE HD1 0.07970 0.00249 . . 21 63 ILE HG2 0.07210 0.00205 . . 22 69 LEU HD1 0.06046 0.00442 . . 23 70 THR HG2 0.05133 0.00097 . . 24 71 MET HE 0.14369 0.00205 . . 25 72 MET HE 0.12743 0.00314 . . 26 73 ALA HB 0.05643 0.00141 . . 27 76 MET HE 0.21417 0.00280 . . 28 79 THR HG2 0.04903 0.00079 . . 29 85 ILE HD1 0.08641 0.00174 . . 30 85 ILE HG2 0.07345 0.00166 . . 31 88 ALA HB 0.03612 0.00135 . . 32 100 ILE HD1 0.06528 0.00324 . . 33 102 ALA HB 0.05784 0.00137 . . 34 103 ALA HB 0.05034 0.00114 . . 35 105 LEU HD1 0.06932 0.00307 . . 36 108 VAL HG1 0.05414 0.00112 . . 37 109 MET HE 0.11580 0.00122 . . 38 110 THR HG2 0.05440 0.00099 . . 39 112 LEU HD2 0.08119 0.00344 . . 40 116 LEU HD1 0.05434 0.00187 . . 41 121 VAL HG1 0.05896 0.00112 . . 42 124 MET HE 0.09513 0.00225 . . 43 125 ILE HD1 0.09715 0.00203 . . 44 125 ILE HG2 0.06139 0.00137 . . 45 128 ALA HB 0.03764 0.00143 . . 46 130 ILE HD1 0.08887 0.00139 . . 47 130 ILE HG2 0.05968 0.00084 . . 48 136 VAL HG1 0.04657 0.00175 . . 49 142 VAL HG2 0.07916 0.00160 . . 50 144 MET HE 0.08581 0.00130 . . 51 145 MET HE 0.14842 0.00213 . . 52 146 THR HG2 0.04782 0.00075 . . 53 147 ALA HB 0.05800 0.00064 . . stop_ save_ save_2H_T2_600MHz_Dz_60C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_4 _Spectrometer_frequency_1H 600 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.07116 0.00283 . . 2 9 ILE HD1 0.10550 0.00205 . . 3 10 ALA HB 0.05727 0.00106 . . 4 15 ALA HB 0.04623 0.00155 . . 5 18 LEU HD2 0.08497 0.00219 . . 6 26 THR HG2 0.03676 0.00076 . . 7 27 ILE HD1 0.05958 0.00253 . . 8 27 ILE HG2 0.06182 0.00156 . . 9 29 THR HG2 0.04722 0.00094 . . 10 34 THR HG2 0.05167 0.00096 . . 11 35 VAL HG1 0.05274 0.00114 . . 12 35 VAL HG2 0.06926 0.00140 . . 13 39 LEU HD1 0.06578 0.00307 . . 14 39 LEU HD2 0.08532 0.00260 . . 15 46 ALA HB 0.05319 0.00100 . . 16 52 ILE HD1 0.12306 0.00254 . . 17 52 ILE HG2 0.05848 0.00117 . . 18 55 VAL HG1 0.04626 0.00073 . . 19 55 VAL HG2 0.05271 0.00089 . . 20 63 ILE HD1 0.07496 0.00200 . . 21 63 ILE HG2 0.07095 0.00185 . . 22 69 LEU HD1 0.06394 0.00369 . . 23 70 THR HG2 0.05216 0.00088 . . 24 71 MET HE 0.15311 0.00202 . . 25 72 MET HE 0.11846 0.00219 . . 26 73 ALA HB 0.05623 0.00116 . . 27 76 MET HE 0.23281 0.00314 . . 28 79 THR HG2 0.05044 0.00064 . . 29 85 ILE HD1 0.09117 0.00176 . . 30 85 ILE HG2 0.07540 0.00147 . . 31 88 ALA HB 0.03585 0.00115 . . 32 100 ILE HD1 0.06867 0.00288 . . 33 102 ALA HB 0.05714 0.00127 . . 34 103 ALA HB 0.05247 0.00105 . . 35 105 LEU HD1 0.07050 0.00242 . . 36 108 VAL HG1 0.05636 0.00104 . . 37 109 MET HE 0.12349 0.00121 . . 38 110 THR HG2 0.05660 0.00096 . . 39 112 LEU HD2 0.08155 0.00298 . . 40 116 LEU HD1 0.06290 0.00189 . . 41 121 VAL HG1 0.06040 0.00099 . . 42 124 MET HE 0.08912 0.00170 . . 43 125 ILE HD1 0.09995 0.00196 . . 44 125 ILE HG2 0.06285 0.00124 . . 45 128 ALA HB 0.03856 0.00118 . . 46 130 ILE HD1 0.09107 0.00138 . . 47 130 ILE HG2 0.06077 0.00079 . . 48 136 VAL HG1 0.04923 0.00155 . . 49 142 VAL HG2 0.07775 0.00145 . . 50 144 MET HE 0.08545 0.00099 . . 51 145 MET HE 0.14747 0.00200 . . 52 146 THR HG2 0.04995 0.00074 . . 53 147 ALA HB 0.06220 0.00066 . . stop_ save_ save_2H_T2_600MHz_Dz_67C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_13 _Spectrometer_frequency_1H 600 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.07951 0.00439 . . 2 9 ILE HD1 0.10774 0.00291 . . 3 10 ALA HB 0.05550 0.00132 . . 4 15 ALA HB 0.04468 0.00217 . . 5 18 LEU HD2 0.08658 0.00312 . . 6 26 THR HG2 0.03726 0.00085 . . 7 27 ILE HD1 0.05825 0.00346 . . 8 27 ILE HG2 0.06037 0.00168 . . 9 29 THR HG2 0.04991 0.00116 . . 10 34 THR HG2 0.05380 0.00117 . . 11 35 VAL HG1 0.05289 0.00150 . . 12 35 VAL HG2 0.06992 0.00190 . . 13 39 LEU HD1 0.05986 0.00319 . . 14 39 LEU HD2 0.08425 0.00333 . . 15 46 ALA HB 0.05294 0.00121 . . 16 52 ILE HD1 0.13292 0.00383 . . 17 52 ILE HG2 0.05865 0.00147 . . 18 55 VAL HG1 0.04833 0.00095 . . 19 55 VAL HG2 0.05714 0.00134 . . 20 63 ILE HD1 0.07573 0.00250 . . 21 63 ILE HG2 0.06832 0.00211 . . 22 69 LEU HD1 0.07205 0.00649 . . 23 70 THR HG2 0.05476 0.00097 . . 24 71 MET HE 0.16329 0.00333 . . 25 72 MET HE 0.12026 0.00447 . . 26 73 ALA HB 0.05593 0.00194 . . 27 76 MET HE 0.27660 0.00874 . . 28 79 THR HG2 0.05239 0.00112 . . 29 85 ILE HD1 0.09758 0.00281 . . 30 85 ILE HG2 0.07529 0.00199 . . 31 88 ALA HB 0.03622 0.00129 . . 32 100 ILE HD1 0.06321 0.00336 . . 33 102 ALA HB 0.05739 0.00155 . . 34 103 ALA HB 0.05128 0.00120 . . 35 105 LEU HD1 0.07256 0.00339 . . 36 108 VAL HG1 0.05739 0.00139 . . 37 109 MET HE 0.12921 0.00214 . . 38 110 THR HG2 0.05644 0.00117 . . 39 112 LEU HD2 0.08047 0.00362 . . 40 116 LEU HD1 0.06580 0.00264 . . 41 121 VAL HG1 0.05975 0.00122 . . 42 124 MET HE 0.08704 0.00332 . . 43 125 ILE HD1 0.10458 0.00291 . . 44 125 ILE HG2 0.06139 0.00146 . . 45 128 ALA HB 0.04014 0.00167 . . 46 130 ILE HD1 0.09660 0.00196 . . 47 130 ILE HG2 0.05923 0.00097 . . 48 136 VAL HG1 0.04688 0.00176 . . 49 142 VAL HG2 0.07628 0.00179 . . 50 144 MET HE 0.08772 0.00225 . . 51 145 MET HE 0.15600 0.00381 . . 52 146 THR HG2 0.05031 0.00092 . . 53 147 ALA HB 0.06355 0.00090 . . stop_ save_ save_2H_T2_600MHz_Dz_73C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_5 _Spectrometer_frequency_1H 600 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.08626 0.00868 . . 2 10 ALA HB 0.05618 0.00203 . . 3 15 ALA HB 0.04303 0.00398 . . 4 18 LEU HD2 0.09407 0.00694 . . 5 26 THR HG2 0.03828 0.00117 . . 6 27 ILE HD1 0.06412 0.00630 . . 7 27 ILE HG2 0.06196 0.00254 . . 8 29 THR HG2 0.05319 0.00170 . . 9 34 THR HG2 0.05449 0.00174 . . 10 35 VAL HG1 0.05473 0.00251 . . 11 35 VAL HG2 0.07187 0.00356 . . 12 39 LEU HD1 0.06162 0.00490 . . 13 39 LEU HD2 0.08625 0.00551 . . 14 46 ALA HB 0.05299 0.00176 . . 15 52 ILE HD1 0.13898 0.00677 . . 16 52 ILE HG2 0.05668 0.00204 . . 17 55 VAL HG1 0.05095 0.00151 . . 18 55 VAL HG2 0.06021 0.00215 . . 19 63 ILE HD1 0.07331 0.00355 . . 20 63 ILE HG2 0.06713 0.00323 . . 21 69 LEU HD1 0.07637 0.01124 . . 22 70 THR HG2 0.06226 0.00129 . . 23 71 MET HE 0.17500 0.00699 . . 24 72 MET HE 0.11699 0.00974 . . 25 73 ALA HB 0.05289 0.00374 . . 26 79 THR HG2 0.05892 0.00242 . . 27 85 ILE HD1 0.10329 0.00576 . . 28 85 ILE HG2 0.07967 0.00350 . . 29 88 ALA HB 0.03582 0.00179 . . 30 100 ILE HD1 0.06259 0.00565 . . 31 102 ALA HB 0.05374 0.00214 . . 32 103 ALA HB 0.05259 0.00179 . . 33 105 LEU HD1 0.07336 0.00596 . . 34 108 VAL HG1 0.05777 0.00233 . . 35 109 MET HE 0.13530 0.00425 . . 36 110 THR HG2 0.05636 0.00179 . . 37 112 LEU HD2 0.07938 0.00552 . . 38 116 LEU HD1 0.06904 0.00449 . . 39 121 VAL HG1 0.06252 0.00208 . . 40 124 MET HE 0.08973 0.00766 . . 41 125 ILE HD1 0.11005 0.00580 . . 42 125 ILE HG2 0.06056 0.00215 . . 43 128 ALA HB 0.04158 0.00298 . . 44 130 ILE HG2 0.06123 0.00158 . . 45 136 VAL HG1 0.05012 0.00320 . . 46 142 VAL HG2 0.07823 0.00288 . . 47 144 MET HE 0.09146 0.00533 . . 48 145 MET HE 0.15412 0.00788 . . 49 146 THR HG2 0.05357 0.00150 . . 50 147 ALA HB 0.06751 0.00141 . . stop_ save_ save_2H_T2_750MHz_Dy_15C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_6 _Spectrometer_frequency_1H 750 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.01127 0.00171 . . 2 9 ILE HD1 0.00884 0.00041 . . 3 10 ALA HB 0.00536 0.00053 . . 4 15 ALA HB 0.00881 0.00338 . . 5 18 LEU HD2 0.00946 0.00115 . . 6 26 THR HG2 0.00604 0.00098 . . 7 27 ILE HD1 0.00781 0.00195 . . 8 27 ILE HG2 0.00684 0.00100 . . 9 29 THR HG2 0.00673 0.00067 . . 10 34 THR HG2 0.00684 0.00059 . . 11 35 VAL HG1 0.00629 0.00073 . . 12 35 VAL HG2 0.00779 0.00069 . . 13 36 MET HE 0.01732 0.00031 . . 14 39 LEU HD1 0.00908 0.00202 . . 15 39 LEU HD2 0.00809 0.00092 . . 16 46 ALA HB 0.00577 0.00044 . . 17 51 MET HE 0.01719 0.00031 . . 18 52 ILE HD1 0.01454 0.00076 . . 19 52 ILE HG2 0.00663 0.00080 . . 20 55 VAL HG1 0.00865 0.00049 . . 21 55 VAL HG2 0.00922 0.00063 . . 22 63 ILE HD1 0.00728 0.00100 . . 23 63 ILE HG2 0.00680 0.00095 . . 24 69 LEU HD1 0.01076 0.00178 . . 25 70 THR HG2 0.00650 0.00053 . . 26 71 MET HE 0.01026 0.00044 . . 27 72 MET HE 0.00820 0.00135 . . 28 73 ALA HB 0.00600 0.00067 . . 29 76 MET HE 0.04095 0.00093 . . 30 79 THR HG2 0.01119 0.00047 . . 31 85 ILE HD1 0.01448 0.00064 . . 32 85 ILE HG2 0.00672 0.00062 . . 33 88 ALA HB 0.00483 0.00112 . . 34 91 VAL HG1 0.00595 0.00086 . . 35 100 ILE HD1 0.00590 0.00142 . . 36 102 ALA HB 0.00558 0.00064 . . 37 103 ALA HB 0.00599 0.00052 . . 38 105 LEU HD1 0.00762 0.00186 . . 39 108 VAL HG1 0.00849 0.00059 . . 40 108 VAL HG2 0.01123 0.00069 . . 41 109 MET HE 0.01288 0.00028 . . 42 110 THR HG2 0.00703 0.00049 . . 43 112 LEU HD2 0.00833 0.00137 . . 44 116 LEU HD1 0.01593 0.00147 . . 45 121 VAL HG1 0.00638 0.00049 . . 46 124 MET HE 0.00764 0.00215 . . 47 125 ILE HD1 0.01030 0.00061 . . 48 125 ILE HG2 0.00571 0.00060 . . 49 128 ALA HB 0.00577 0.00164 . . 50 130 ILE HD1 0.01358 0.00039 . . 51 130 ILE HG2 0.00765 0.00030 . . 52 136 VAL HG1 0.00692 0.00161 . . 53 142 VAL HG1 0.00646 0.00050 . . 54 142 VAL HG2 0.00788 0.00056 . . 55 144 MET HE 0.01100 0.00108 . . 56 145 MET HE 0.01756 0.00070 . . 57 146 THR HG2 0.00823 0.00039 . . 58 147 ALA HB 0.01056 0.00026 . . stop_ save_ save_2H_T2_750MHz_Dy_22C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 750 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.01241 0.00088 . . 2 9 ILE HD1 0.01051 0.00029 . . 3 10 ALA HB 0.00648 0.00032 . . 4 15 ALA HB 0.00693 0.00104 . . 5 18 LEU HD2 0.01235 0.00080 . . 6 26 THR HG2 0.00749 0.00051 . . 7 27 ILE HD1 0.00786 0.00094 . . 8 27 ILE HG2 0.00815 0.00059 . . 9 29 THR HG2 0.00808 0.00041 . . 10 34 THR HG2 0.00776 0.00035 . . 11 35 VAL HG1 0.00788 0.00046 . . 12 35 VAL HG2 0.00880 0.00042 . . 13 36 MET HE 0.01822 0.00018 . . 14 39 LEU HD1 0.01046 0.00113 . . 15 39 LEU HD2 0.01043 0.00067 . . 16 46 ALA HB 0.00668 0.00027 . . 17 51 MET HE 0.01932 0.00020 . . 18 52 ILE HD1 0.01597 0.00045 . . 19 52 ILE HG2 0.00688 0.00041 . . 20 55 VAL HG1 0.00979 0.00030 . . 21 55 VAL HG2 0.01111 0.00040 . . 22 63 ILE HD1 0.00787 0.00054 . . 23 63 ILE HG2 0.00769 0.00052 . . 24 69 LEU HD1 0.01434 0.00143 . . 25 70 THR HG2 0.00793 0.00033 . . 26 71 MET HE 0.01334 0.00027 . . 27 72 MET HE 0.00776 0.00045 . . 28 73 ALA HB 0.00711 0.00036 . . 29 76 MET HE 0.04111 0.00048 . . 30 79 THR HG2 0.01194 0.00028 . . 31 85 ILE HD1 0.01722 0.00047 . . 32 85 ILE HG2 0.00873 0.00040 . . 33 88 ALA HB 0.00633 0.00062 . . 34 91 VAL HG1 0.00699 0.00046 . . 35 100 ILE HD1 0.00818 0.00097 . . 36 102 ALA HB 0.00639 0.00037 . . 37 103 ALA HB 0.00658 0.00032 . . 38 105 LEU HD1 0.00877 0.00093 . . 39 108 VAL HG1 0.01006 0.00038 . . 40 108 VAL HG2 0.01170 0.00037 . . 41 109 MET HE 0.01604 0.00019 . . 42 110 THR HG2 0.00805 0.00029 . . 43 112 LEU HD2 0.00859 0.00070 . . 44 116 LEU HD1 0.01697 0.00085 . . 45 121 VAL HG1 0.00837 0.00034 . . 46 124 MET HE 0.00754 0.00063 . . 47 125 ILE HD1 0.01251 0.00039 . . 48 125 ILE HG2 0.00728 0.00038 . . 49 128 ALA HB 0.00627 0.00073 . . 50 130 ILE HD1 0.01531 0.00028 . . 51 130 ILE HG2 0.00929 0.00020 . . 52 136 VAL HG1 0.00796 0.00082 . . 53 142 VAL HG1 0.00792 0.00032 . . 54 142 VAL HG2 0.00908 0.00036 . . 55 144 MET HE 0.01403 0.00047 . . 56 145 MET HE 0.02060 0.00039 . . 57 146 THR HG2 0.00937 0.00025 . . 58 147 ALA HB 0.01219 0.00017 . . stop_ save_ save_2H_T2_750MHz_Dy_35C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_2 _Spectrometer_frequency_1H 750 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.01858 0.00117 . . 2 9 ILE HD1 0.01493 0.00040 . . 3 10 ALA HB 0.00863 0.00033 . . 4 15 ALA HB 0.00947 0.00085 . . 5 18 LEU HD2 0.01920 0.00104 . . 6 26 THR HG2 0.00980 0.00046 . . 7 27 ILE HD1 0.01023 0.00088 . . 8 27 ILE HG2 0.01022 0.00056 . . 9 29 THR HG2 0.01112 0.00043 . . 10 34 THR HG2 0.01112 0.00043 . . 11 35 VAL HG1 0.01035 0.00046 . . 12 35 VAL HG2 0.01216 0.00048 . . 13 36 MET HE 0.02570 0.00029 . . 14 39 LEU HD1 0.01381 0.00119 . . 15 39 LEU HD2 0.01493 0.00082 . . 16 46 ALA HB 0.00894 0.00031 . . 17 51 MET HE 0.02861 0.00034 . . 18 52 ILE HD1 0.02226 0.00062 . . 19 52 ILE HG2 0.00948 0.00040 . . 20 55 VAL HG1 0.01384 0.00037 . . 21 55 VAL HG2 0.01529 0.00048 . . 22 63 ILE HD1 0.01124 0.00059 . . 23 63 ILE HG2 0.01015 0.00052 . . 24 69 LEU HD1 0.01811 0.00171 . . 25 70 THR HG2 0.01104 0.00037 . . 26 71 MET HE 0.01938 0.00035 . . 27 72 MET HE 0.01183 0.00045 . . 28 73 ALA HB 0.00947 0.00041 . . 29 76 MET HE 0.05719 0.00091 . . 30 79 THR HG2 0.01703 0.00042 . . 31 85 ILE HD1 0.02142 0.00060 . . 32 85 ILE HG2 0.01209 0.00046 . . 33 88 ALA HB 0.00787 0.00053 . . 34 91 VAL HG1 0.00948 0.00047 . . 35 100 ILE HD1 0.00937 0.00073 . . 36 102 ALA HB 0.00834 0.00037 . . 37 103 ALA HB 0.00854 0.00034 . . 38 105 LEU HD1 0.01211 0.00088 . . 39 108 VAL HG1 0.01313 0.00045 . . 40 108 VAL HG2 0.01610 0.00046 . . 41 109 MET HE 0.02387 0.00033 . . 42 110 THR HG2 0.01128 0.00037 . . 43 112 LEU HD2 0.01261 0.00088 . . 44 116 LEU HD1 0.02362 0.00129 . . 45 121 VAL HG1 0.01129 0.00037 . . 46 124 MET HE 0.01021 0.00043 . . 47 125 ILE HD1 0.01848 0.00053 . . 48 125 ILE HG2 0.00922 0.00036 . . 49 128 ALA HB 0.00814 0.00063 . . 50 130 ILE HD1 0.01986 0.00040 . . 51 130 ILE HG2 0.01260 0.00026 . . 52 136 VAL HG1 0.00881 0.00058 . . 53 142 VAL HG1 0.01029 0.00032 . . 54 142 VAL HG2 0.01348 0.00046 . . 55 144 MET HE 0.02001 0.00049 . . 56 145 MET HE 0.02805 0.00055 . . 57 146 THR HG2 0.01317 0.00035 . . 58 147 ALA HB 0.01657 0.00026 . . stop_ save_ save_2H_T2_750MHz_Dy_47C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_3 _Spectrometer_frequency_1H 750 _T2_coherence_type Dy _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.02465 0.00239 . . 2 9 ILE HD1 0.02130 0.00088 . . 3 10 ALA HB 0.01151 0.00051 . . 4 15 ALA HB 0.01188 0.00116 . . 5 18 LEU HD2 0.02488 0.00178 . . 6 26 THR HG2 0.01283 0.00061 . . 7 27 ILE HD1 0.01263 0.00120 . . 8 27 ILE HG2 0.01266 0.00072 . . 9 29 THR HG2 0.01486 0.00067 . . 10 34 THR HG2 0.01438 0.00062 . . 11 35 VAL HG1 0.01358 0.00070 . . 12 35 VAL HG2 0.01615 0.00081 . . 13 39 LEU HD1 0.01827 0.00204 . . 14 39 LEU HD2 0.01921 0.00139 . . 15 46 ALA HB 0.01161 0.00050 . . 16 52 ILE HD1 0.02880 0.00129 . . 17 52 ILE HG2 0.01298 0.00062 . . 18 55 VAL HG1 0.01808 0.00067 . . 19 55 VAL HG2 0.01991 0.00087 . . 20 63 ILE HD1 0.01433 0.00087 . . 21 63 ILE HG2 0.01235 0.00066 . . 22 69 LEU HD1 0.02789 0.00476 . . 23 70 THR HG2 0.01474 0.00063 . . 24 71 MET HE 0.02870 0.00082 . . 25 72 MET HE 0.01687 0.00081 . . 26 73 ALA HB 0.01227 0.00065 . . 27 76 MET HE 0.07900 0.00344 . . 28 79 THR HG2 0.02159 0.00086 . . 29 85 ILE HD1 0.02797 0.00131 . . 30 85 ILE HG2 0.01570 0.00075 . . 31 88 ALA HB 0.01082 0.00075 . . 32 100 ILE HD1 0.01210 0.00103 . . 33 102 ALA HB 0.01090 0.00057 . . 34 103 ALA HB 0.01198 0.00060 . . 35 105 LEU HD1 0.01614 0.00134 . . 36 108 VAL HG1 0.01612 0.00072 . . 37 108 VAL HG2 0.01900 0.00064 . . 38 109 MET HE 0.03132 0.00076 . . 39 110 THR HG2 0.01440 0.00059 . . 40 112 LEU HD2 0.01649 0.00141 . . 41 116 LEU HD1 0.02955 0.00268 . . 42 121 VAL HG1 0.01454 0.00057 . . 43 124 MET HE 0.01329 0.00063 . . 44 125 ILE HD1 0.02525 0.00113 . . 45 125 ILE HG2 0.01212 0.00055 . . 46 128 ALA HB 0.00976 0.00074 . . 47 130 ILE HD1 0.02697 0.00097 . . 48 130 ILE HG2 0.01697 0.00050 . . 49 136 VAL HG1 0.01087 0.00076 . . 50 142 VAL HG2 0.01647 0.00068 . . 51 144 MET HE 0.02739 0.00112 . . 52 145 MET HE 0.03356 0.00104 . . 53 146 THR HG2 0.01622 0.00056 . . 54 147 ALA HB 0.02131 0.00055 . . stop_ save_ save_2H_T2_750MHz_Dz_15C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_6 _Spectrometer_frequency_1H 750 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.03586 0.00725 . . 2 9 ILE HD1 0.11133 0.01025 . . 3 10 ALA HB 0.04779 0.00592 . . 4 15 ALA HB 0.03558 0.01842 . . 5 18 LEU HD2 0.05846 0.01116 . . 6 26 THR HG2 0.02043 0.00438 . . 7 27 ILE HD1 0.07129 0.02883 . . 8 27 ILE HG2 0.05571 0.01153 . . 9 29 THR HG2 0.03158 0.00441 . . 10 34 THR HG2 0.03348 0.00366 . . 11 35 VAL HG1 0.03402 0.00527 . . 12 35 VAL HG2 0.07095 0.00958 . . 13 36 MET HE 0.15746 0.00616 . . 14 39 LEU HD1 0.03581 0.01019 . . 15 39 LEU HD2 0.05782 0.00959 . . 16 46 ALA HB 0.04003 0.00365 . . 17 51 MET HE 0.15672 0.00603 . . 18 52 ILE HD1 0.09581 0.00844 . . 19 52 ILE HG2 0.04571 0.00728 . . 20 55 VAL HG1 0.03294 0.00256 . . 21 55 VAL HG2 0.03879 0.00380 . . 22 63 ILE HD1 0.09756 0.02380 . . 23 63 ILE HG2 0.08302 0.02031 . . 24 69 LEU HD1 0.05355 0.01394 . . 25 70 THR HG2 0.03972 0.00387 . . 26 71 MET HE 0.16058 0.01751 . . 27 72 MET HE 0.14205 0.04635 . . 28 73 ALA HB 0.04976 0.00699 . . 29 76 MET HE 0.13031 0.00331 . . 30 79 THR HG2 0.04629 0.00249 . . 31 85 ILE HD1 0.07905 0.00529 . . 32 85 ILE HG2 0.06145 0.00831 . . 33 88 ALA HB 0.03379 0.01250 . . 34 91 VAL HG1 0.03527 0.00635 . . 35 100 ILE HD1 0.12016 0.06885 . . 36 102 ALA HB 0.04504 0.00605 . . 37 103 ALA HB 0.04016 0.00420 . . 38 105 LEU HD1 0.04906 0.01597 . . 39 108 VAL HG1 0.03598 0.00336 . . 40 108 VAL HG2 0.06924 0.00627 . . 41 109 MET HE 0.12653 0.00569 . . 42 110 THR HG2 0.04094 0.00345 . . 43 112 LEU HD2 0.06206 0.01482 . . 44 116 LEU HD1 0.03211 0.00371 . . 45 121 VAL HG1 0.05119 0.00575 . . 46 124 MET HE 0.27312 0.26619 . . 47 125 ILE HD1 0.07708 0.00788 . . 48 125 ILE HG2 0.04855 0.00645 . . 49 128 ALA HB 0.02993 0.01128 . . 50 130 ILE HD1 0.09026 0.00407 . . 51 130 ILE HG2 0.05706 0.00297 . . 52 136 VAL HG1 0.04006 0.01190 . . 53 142 VAL HG1 0.02948 0.00299 . . 54 142 VAL HG2 0.08917 0.01139 . . 55 144 MET HE 0.12716 0.02575 . . 56 145 MET HE 0.12176 0.00850 . . 57 146 THR HG2 0.03886 0.00236 . . 58 147 ALA HB 0.04083 0.00127 . . stop_ save_ save_2H_T2_750MHz_Dz_22C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 750 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.04355 0.00448 . . 2 9 ILE HD1 0.12200 0.00615 . . 3 10 ALA HB 0.05313 0.00309 . . 4 15 ALA HB 0.04827 0.00898 . . 5 18 LEU HD2 0.07170 0.00648 . . 6 26 THR HG2 0.02550 0.00248 . . 7 27 ILE HD1 0.08677 0.01658 . . 8 27 ILE HG2 0.06211 0.00600 . . 9 29 THR HG2 0.03376 0.00236 . . 10 34 THR HG2 0.04142 0.00240 . . 11 35 VAL HG1 0.03941 0.00310 . . 12 35 VAL HG2 0.08044 0.00546 . . 13 36 MET HE 0.19001 0.00456 . . 14 39 LEU HD1 0.05206 0.00754 . . 15 39 LEU HD2 0.07003 0.00612 . . 16 46 ALA HB 0.04782 0.00237 . . 17 51 MET HE 0.17894 0.00409 . . 18 52 ILE HD1 0.10883 0.00513 . . 19 52 ILE HG2 0.05014 0.00383 . . 20 55 VAL HG1 0.03611 0.00155 . . 21 55 VAL HG2 0.04223 0.00206 . . 22 63 ILE HD1 0.10961 0.01276 . . 23 63 ILE HG2 0.09393 0.01022 . . 24 69 LEU HD1 0.05343 0.00652 . . 25 70 THR HG2 0.04536 0.00225 . . 26 71 MET HE 0.17839 0.00905 . . 27 72 MET HE 0.18858 0.02469 . . 28 73 ALA HB 0.05532 0.00347 . . 29 76 MET HE 0.15423 0.00250 . . 30 79 THR HG2 0.04714 0.00144 . . 31 85 ILE HD1 0.08987 0.00351 . . 32 85 ILE HG2 0.06815 0.00442 . . 33 88 ALA HB 0.03691 0.00551 . . 34 91 VAL HG1 0.04071 0.00360 . . 35 100 ILE HD1 0.08835 0.01657 . . 36 102 ALA HB 0.05813 0.00405 . . 37 103 ALA HB 0.04504 0.00254 . . 38 105 LEU HD1 0.05729 0.00839 . . 39 108 VAL HG1 0.04197 0.00216 . . 40 108 VAL HG2 0.07203 0.00324 . . 41 109 MET HE 0.13417 0.00319 . . 42 110 THR HG2 0.04644 0.00206 . . 43 112 LEU HD2 0.07660 0.00940 . . 44 116 LEU HD1 0.03691 0.00249 . . 45 121 VAL HG1 0.05120 0.00269 . . 46 124 MET HE 0.17254 0.02928 . . 47 125 ILE HD1 0.09000 0.00491 . . 48 125 ILE HG2 0.05980 0.00397 . . 49 128 ALA HB 0.03365 0.00521 . . 50 130 ILE HD1 0.09696 0.00265 . . 51 130 ILE HG2 0.06341 0.00176 . . 52 136 VAL HG1 0.04036 0.00568 . . 53 142 VAL HG1 0.03375 0.00184 . . 54 142 VAL HG2 0.08855 0.00524 . . 55 144 MET HE 0.12384 0.00761 . . 56 145 MET HE 0.15992 0.00594 . . 57 146 THR HG2 0.04333 0.00153 . . 58 147 ALA HB 0.04710 0.00091 . . stop_ save_ save_2H_T2_750MHz_Dz_35C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_2 _Spectrometer_frequency_1H 750 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.05978 0.00482 . . 2 9 ILE HD1 0.13592 0.00629 . . 3 10 ALA HB 0.06264 0.00272 . . 4 15 ALA HB 0.04518 0.00470 . . 5 18 LEU HD2 0.08455 0.00577 . . 6 26 THR HG2 0.03041 0.00170 . . 7 27 ILE HD1 0.07149 0.00799 . . 8 27 ILE HG2 0.07071 0.00494 . . 9 29 THR HG2 0.04395 0.00211 . . 10 34 THR HG2 0.05096 0.00226 . . 11 35 VAL HG1 0.04820 0.00260 . . 12 35 VAL HG2 0.09010 0.00503 . . 13 36 MET HE 0.20604 0.00499 . . 14 39 LEU HD1 0.05491 0.00612 . . 15 39 LEU HD2 0.09102 0.00682 . . 16 46 ALA HB 0.05730 0.00224 . . 17 51 MET HE 0.20632 0.00501 . . 18 52 ILE HD1 0.13532 0.00619 . . 19 52 ILE HG2 0.05829 0.00302 . . 20 55 VAL HG1 0.04376 0.00146 . . 21 55 VAL HG2 0.05032 0.00199 . . 22 63 ILE HD1 0.12566 0.01093 . . 23 63 ILE HG2 0.08959 0.00640 . . 24 69 LEU HD1 0.06034 0.00702 . . 25 70 THR HG2 0.05536 0.00226 . . 26 71 MET HE 0.18393 0.00735 . . 27 72 MET HE 0.20916 0.01923 . . 28 73 ALA HB 0.06293 0.00304 . . 29 76 MET HE 0.20662 0.00447 . . 30 79 THR HG2 0.05175 0.00145 . . 31 85 ILE HD1 0.09899 0.00386 . . 32 85 ILE HG2 0.08333 0.00443 . . 33 88 ALA HB 0.03574 0.00297 . . 34 91 VAL HG1 0.05059 0.00286 . . 35 100 ILE HD1 0.08776 0.01007 . . 36 102 ALA HB 0.06495 0.00322 . . 37 103 ALA HB 0.05604 0.00238 . . 38 105 LEU HD1 0.06839 0.00641 . . 39 108 VAL HG1 0.05198 0.00217 . . 40 108 VAL HG2 0.08567 0.00342 . . 41 109 MET HE 0.14183 0.00316 . . 42 110 THR HG2 0.05905 0.00228 . . 43 112 LEU HD2 0.08353 0.00794 . . 44 116 LEU HD1 0.04867 0.00303 . . 45 121 VAL HG1 0.06138 0.00250 . . 46 124 MET HE 0.16867 0.01350 . . 47 125 ILE HD1 0.10248 0.00431 . . 48 125 ILE HG2 0.07135 0.00346 . . 49 128 ALA HB 0.03635 0.00322 . . 50 130 ILE HD1 0.10707 0.00310 . . 51 130 ILE HG2 0.07149 0.00183 . . 52 136 VAL HG1 0.04503 0.00363 . . 53 142 VAL HG1 0.04203 0.00162 . . 54 142 VAL HG2 0.10294 0.00516 . . 55 144 MET HE 0.12898 0.00522 . . 56 145 MET HE 0.18266 0.00677 . . 57 146 THR HG2 0.05177 0.00157 . . 58 147 ALA HB 0.05685 0.00106 . . stop_ save_ save_2H_T2_750MHz_Dz_47C _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_3 _Spectrometer_frequency_1H 750 _T2_coherence_type Dz _T2_value_units s _Mol_system_component_name Calmodulin _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 4 LEU HD1 0.06673 0.00760 . . 2 9 ILE HD1 0.12310 0.00877 . . 3 10 ALA HB 0.06315 0.00358 . . 4 15 ALA HB 0.04767 0.00563 . . 5 18 LEU HD2 0.08625 0.00763 . . 6 26 THR HG2 0.03349 0.00201 . . 7 27 ILE HD1 0.07374 0.01060 . . 8 27 ILE HG2 0.07052 0.00576 . . 9 29 THR HG2 0.04548 0.00260 . . 10 34 THR HG2 0.05198 0.00280 . . 11 35 VAL HG1 0.05121 0.00344 . . 12 35 VAL HG2 0.08284 0.00605 . . 13 39 LEU HD1 0.06095 0.00873 . . 14 39 LEU HD2 0.09001 0.00913 . . 15 46 ALA HB 0.05896 0.00322 . . 16 52 ILE HD1 0.13634 0.01031 . . 17 52 ILE HG2 0.06219 0.00399 . . 18 55 VAL HG1 0.04511 0.00203 . . 19 55 VAL HG2 0.05260 0.00287 . . 20 63 ILE HD1 0.10171 0.01069 . . 21 63 ILE HG2 0.08937 0.00794 . . 22 69 LEU HD1 0.06778 0.01238 . . 23 70 THR HG2 0.05600 0.00290 . . 24 71 MET HE 0.16957 0.01005 . . 25 72 MET HE 0.16787 0.01963 . . 26 73 ALA HB 0.06686 0.00482 . . 27 76 MET HE 0.21689 0.01187 . . 28 79 THR HG2 0.05200 0.00229 . . 29 85 ILE HD1 0.09740 0.00612 . . 30 85 ILE HG2 0.08522 0.00614 . . 31 88 ALA HB 0.03738 0.00339 . . 32 100 ILE HD1 0.07787 0.01041 . . 33 102 ALA HB 0.06623 0.00459 . . 34 103 ALA HB 0.05825 0.00360 . . 35 105 LEU HD1 0.07047 0.00793 . . 36 108 VAL HG1 0.05548 0.00323 . . 37 108 VAL HG2 0.08207 0.00399 . . 38 109 MET HE 0.13194 0.00509 . . 39 110 THR HG2 0.06122 0.00321 . . 40 112 LEU HD2 0.08447 0.01052 . . 41 116 LEU HD1 0.05774 0.00543 . . 42 121 VAL HG1 0.06468 0.00354 . . 43 124 MET HE 0.12900 0.01264 . . 44 125 ILE HD1 0.10523 0.00708 . . 45 125 ILE HG2 0.06963 0.00440 . . 46 128 ALA HB 0.03987 0.00396 . . 47 130 ILE HD1 0.10159 0.00515 . . 48 130 ILE HG2 0.06925 0.00273 . . 49 136 VAL HG1 0.04674 0.00430 . . 50 142 VAL HG2 0.09140 0.00604 . . 51 144 MET HE 0.10960 0.00677 . . 52 145 MET HE 0.17039 0.01018 . . 53 146 THR HG2 0.05305 0.00229 . . 54 147 ALA HB 0.06149 0.00188 . . stop_ save_ save_heteronuclear_NOE_22C _Saveframe_category heteronuclear_NOE _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 500 _Mol_system_component_name Calmodulin _Atom_one_atom_name H _Atom_two_atom_name N _Heteronuclear_NOE_value_type 'not reported' _NOE_reference_value . _NOE_reference_description . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Heteronuclear_NOE_value _Heteronuclear_NOE_value_error 4 LEU 0.096874 0.023625 5 THR 0.615117 0.045755 6 GLU 0.707607 0.041585 7 GLU 0.737742 0.037421 8 GLN 0.736831 0.039227 11 GLU 0.767349 0.039450 13 LYS 0.774630 0.049158 16 PHE 0.815762 0.057303 18 LEU 0.787322 0.043738 19 PHE 0.787025 0.054757 20 ASP 0.788865 0.052799 21 LYS 0.784673 0.042365 23 GLY 0.817293 0.031393 24 ASP 0.811372 0.031792 26 THR 0.823287 0.035512 27 ILE 0.786955 0.068994 28 THR 0.817086 0.070255 29 THR 0.802064 0.052462 31 GLU 0.782744 0.054067 33 GLY 0.799788 0.058473 34 THR 0.780437 0.039243 36 MET 0.782533 0.052983 37 ARG 0.807620 0.053656 39 LEU 0.774908 0.042638 40 GLY 0.717391 0.036040 42 ASN 0.662789 0.058037 44 THR 0.768802 0.053915 45 GLU 0.778910 0.043858 47 GLU 0.780758 0.036487 49 GLN 0.750428 0.038707 52 ILE 0.763909 0.048229 53 ASN 0.759157 0.039964 54 GLU 0.705993 0.030196 55 VAL 0.745615 0.050387 57 ALA 0.616654 0.033224 60 ASP 0.796292 0.028701 62 THR 0.825501 0.028959 63 ILE 0.775201 0.068277 64 ASP 0.799787 0.060571 65 PHE 0.800112 0.074985 67 GLU 0.818352 0.089924 69 LEU 0.786299 0.058541 70 THR 0.785369 0.040830 71 MET 0.776612 0.047784 74 ARG 0.642694 0.049236 75 LYS 0.573414 0.051572 77 LYS 0.603125 0.065378 80 ASP 0.719040 0.058004 81 SER 0.699594 0.041178 87 GLU 0.776531 0.049121 89 PHE 0.842915 0.050745 91 VAL 0.819281 0.041952 92 PHE 0.844587 0.058802 93 ASP 0.779970 0.043155 94 LYS 0.767491 0.038584 96 GLY 0.782614 0.033724 100 ILE 0.777135 0.056569 101 SER 0.790540 0.066046 102 ALA 0.848416 0.048214 105 LEU 0.789617 0.048993 106 ARG 0.772722 0.049636 109 MET 0.759067 0.048813 110 THR 0.803098 0.052758 111 ASN 0.791395 0.044029 112 LEU 0.732722 0.041007 113 GLY 0.746673 0.043747 115 LYS 0.375838 0.028407 116 LEU 0.287636 0.023495 117 THR 0.632516 0.054108 118 ASP 0.806684 0.049200 120 GLU 0.746346 0.040195 123 GLU 0.746925 0.035164 125 ILE 0.796991 0.054674 126 ARG 0.775742 0.043994 127 GLU 0.762911 0.038064 128 ALA 0.764589 0.042126 129 ASP 0.776371 0.038382 130 ILE 0.633136 0.040959 131 ASP 0.811018 0.038092 133 ASP 0.803934 0.030298 134 GLY 0.774951 0.038965 135 GLN 0.768949 0.029347 136 VAL 0.782341 0.055852 137 ASN 0.794373 0.058339 139 GLU 0.833845 0.033204 143 GLN 0.768214 0.036672 144 MET 0.798399 0.043024 145 MET 0.772209 0.050667 146 THR 0.689765 0.037619 147 ALA 0.444625 0.023884 148 LYS 0.098175 0.012226 stop_ save_ save_heteronuclear_NOE_35C _Saveframe_category heteronuclear_NOE _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_2 _Spectrometer_frequency_1H 500 _Mol_system_component_name Calmodulin _Atom_one_atom_name H _Atom_two_atom_name N _Heteronuclear_NOE_value_type . _NOE_reference_value . _NOE_reference_description . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Heteronuclear_NOE_value _Heteronuclear_NOE_value_error 4 LEU 0.104953 0.021449 5 THR 0.659044 0.029264 6 GLU 0.725173 0.034175 7 GLU 0.734617 0.029699 8 GLN 0.735387 0.026469 9 ILE 0.725907 0.030640 11 GLU 0.788960 0.024987 12 PHE 0.785223 0.029641 13 LYS 0.803265 0.031387 15 ALA 0.768352 0.027586 16 PHE 0.778733 0.033157 18 LEU 0.802743 0.027429 19 PHE 0.784164 0.032977 20 ASP 0.766354 0.030581 21 LYS 0.749426 0.026216 23 GLY 0.814275 0.022185 24 ASP 0.797381 0.022445 26 THR 0.793302 0.024807 27 ILE 0.772852 0.039409 28 THR 0.753661 0.041605 29 THR 0.807313 0.035123 30 LYS 0.848592 0.034025 31 GLU 0.774899 0.033926 32 LEU 0.803680 0.032659 33 GLY 0.808318 0.036650 36 MET 0.801618 0.032171 37 ARG 0.782519 0.033138 39 LEU 0.740398 0.027027 40 GLY 0.710063 0.025320 42 ASN 0.642753 0.089040 44 THR 0.783710 0.034274 45 GLU 0.830068 0.035637 47 GLU 0.755494 0.025904 49 GLN 0.776013 0.027748 51 MET 0.793287 0.026572 52 ILE 0.771936 0.028717 53 ASN 0.767911 0.027468 54 GLU 0.736953 0.020958 55 VAL 0.771073 0.033421 57 ALA 0.667218 0.024605 60 ASP 0.789651 0.021480 62 THR 0.794697 0.021501 63 ILE 0.773812 0.042029 64 ASP 0.788772 0.039946 65 PHE 0.823570 0.041560 67 GLU 0.796830 0.043403 68 PHE 0.758486 0.037209 69 LEU 0.790383 0.035676 70 THR 0.790219 0.026828 71 MET 0.793393 0.030457 74 ARG 0.705933 0.027613 75 LYS 0.585240 0.044602 77 LYS 0.634025 0.058013 80 ASP 0.645322 0.037955 81 SER 0.718623 0.044050 85 ILE 0.754672 0.030897 86 ARG 0.764493 0.031089 87 GLU 0.767158 0.032545 89 PHE 0.794250 0.032487 91 VAL 0.783710 0.025105 92 PHE 0.813767 0.034679 93 ASP 0.762611 0.027491 94 LYS 0.761742 0.023857 96 GLY 0.785717 0.022619 97 ASN 0.812697 0.022167 100 ILE 0.800311 0.034081 101 SER 0.830839 0.041881 102 ALA 0.802882 0.029380 103 ALA 0.834630 0.027025 105 LEU 0.787334 0.030933 106 ARG 0.814082 0.032207 109 MET 0.744994 0.030829 110 THR 0.810429 0.033694 111 ASN 0.796771 0.028878 112 LEU 0.752308 0.027734 113 GLY 0.759631 0.029814 114 GLU 0.749864 0.024393 115 LYS 0.422813 0.026974 116 LEU 0.323765 0.018650 117 THR 0.641120 0.034923 118 ASP 0.781778 0.048363 119 GLU 0.796061 0.028175 120 GLU 0.751644 0.025688 123 GLU 0.758540 0.023896 125 ILE 0.753257 0.032171 126 ARG 0.745681 0.027433 127 GLU 0.822423 0.026952 128 ALA 0.769023 0.027556 130 ILE 0.679486 0.029127 131 ASP 0.812307 0.026354 133 ASP 0.808983 0.022898 134 GLY 0.809451 0.027141 135 GLN 0.803610 0.021577 136 VAL 0.776585 0.033627 137 ASN 0.806114 0.035669 138 TYR 0.803809 0.030107 139 GLU 0.783149 0.021423 141 PHE 0.810320 0.034081 142 VAL 0.790172 0.026470 143 GLN 0.786984 0.023817 144 MET 0.769536 0.026075 145 MET 0.782238 0.029903 146 THR 0.742481 0.027138 147 ALA 0.499965 0.022111 148 LYS -0.018884 0.011416 stop_ save_ save_heteronuclear_NOE_47C _Saveframe_category heteronuclear_NOE _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_3 _Spectrometer_frequency_1H 500 _Mol_system_component_name Calmodulin _Atom_one_atom_name H _Atom_two_atom_name N _Heteronuclear_NOE_value_type . _NOE_reference_value . _NOE_reference_description . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Heteronuclear_NOE_value _Heteronuclear_NOE_value_error 4 LEU 0.113573 0.030951 5 THR 0.664351 0.024772 6 GLU 0.783834 0.050067 7 GLU 0.766153 0.033260 8 GLN 0.713166 0.019732 9 ILE 0.745391 0.021626 12 PHE 0.736035 0.021656 13 LYS 0.738729 0.021548 15 ALA 0.718083 0.021034 16 PHE 0.759740 0.023010 18 LEU 0.783343 0.018791 19 PHE 0.738243 0.024246 21 LYS 0.706821 0.018326 23 GLY 0.743644 0.016014 25 GLY 0.808926 0.024030 26 THR 0.822365 0.020834 27 ILE 0.769175 0.028723 28 THR 0.776708 0.030778 29 THR 0.792988 0.030448 30 LYS 0.864775 0.053943 31 GLU 0.792266 0.023300 32 LEU 0.812675 0.023862 33 GLY 0.751891 0.025108 34 THR 0.774492 0.019045 37 ARG 0.799984 0.024495 39 LEU 0.724596 0.020316 40 GLY 0.753512 0.022285 44 THR 0.761659 0.024220 45 GLU 0.825346 0.044316 47 GLU 0.736943 0.018371 49 GLN 0.774279 0.018609 51 MET 0.735328 0.019215 52 ILE 0.731316 0.019665 55 VAL 0.737953 0.025813 57 ALA 0.744747 0.019305 59 GLY 0.796790 0.017370 60 ASP 0.825721 0.018823 61 GLY 0.757119 0.021469 62 THR 0.800822 0.018044 63 ILE 0.766368 0.030081 64 ASP 0.784747 0.029743 65 PHE 0.813346 0.028985 67 GLU 0.772207 0.027101 69 LEU 0.772350 0.026314 70 THR 0.793870 0.019860 71 MET 0.776455 0.022383 74 ARG 0.653150 0.021584 75 LYS 0.561235 0.079971 85 ILE 0.729802 0.023558 87 GLU 0.776362 0.023243 89 PHE 0.779054 0.022735 91 VAL 0.766740 0.019422 92 PHE 0.784135 0.024228 93 ASP 0.778227 0.020916 94 LYS 0.719786 0.016905 96 GLY 0.792080 0.017432 97 ASN 0.779920 0.018325 98 GLY 0.807251 0.024679 100 ILE 0.777944 0.028002 101 SER 0.823903 0.030377 102 ALA 0.764640 0.021003 106 ARG 0.821665 0.021647 109 MET 0.777855 0.022138 111 ASN 0.777304 0.020909 112 LEU 0.744934 0.020915 113 GLY 0.732645 0.022858 114 GLU 0.742791 0.020457 115 LYS 0.456528 0.035312 116 LEU 0.366674 0.017234 117 THR 0.611387 0.025854 119 GLU 0.825283 0.028953 120 GLU 0.735274 0.017874 123 GLU 0.728699 0.017268 125 ILE 0.786132 0.023389 126 ARG 0.745857 0.020428 127 GLU 0.742970 0.018579 128 ALA 0.778563 0.020243 129 ASP 0.767206 0.018383 130 ILE 0.696071 0.020780 132 GLY 0.766202 0.015827 133 ASP 0.795658 0.017625 134 GLY 0.795213 0.020232 135 GLN 0.740044 0.018195 136 VAL 0.779685 0.024774 137 ASN 0.760620 0.026236 138 TYR 0.814129 0.022563 142 VAL 0.778961 0.020690 143 GLN 0.768184 0.017196 144 MET 0.729218 0.019430 145 MET 0.731045 0.022581 146 THR 0.729409 0.021412 147 ALA 0.546860 0.028275 148 LYS 0.073516 0.011736 stop_ save_ save_heteronuclear_NOE_60C _Saveframe_category heteronuclear_NOE _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_4 _Spectrometer_frequency_1H 500 _Mol_system_component_name Calmodulin _Atom_one_atom_name H _Atom_two_atom_name N _Heteronuclear_NOE_value_type . _NOE_reference_value . _NOE_reference_description . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Heteronuclear_NOE_value _Heteronuclear_NOE_value_error 4 LEU 0.127484 0.073901 5 THR 0.671591 0.032139 7 GLU 0.788237 0.060600 8 GLN 0.710079 0.021509 9 ILE 0.721854 0.022435 11 GLU 0.698206 0.019233 12 PHE 0.698947 0.025878 15 ALA 0.713604 0.021842 16 PHE 0.729854 0.022468 19 PHE 0.706129 0.026392 21 LYS 0.752976 0.020861 23 GLY 0.755769 0.016578 25 GLY 0.788975 0.024328 26 THR 0.787459 0.021731 27 ILE 0.743926 0.028819 28 THR 0.777840 0.030103 29 THR 0.818800 0.053171 31 GLU 0.755799 0.021734 32 LEU 0.766233 0.023247 33 GLY 0.722558 0.025884 37 ARG 0.783986 0.027303 39 LEU 0.774984 0.022266 40 GLY 0.776850 0.025943 44 THR 0.729516 0.025755 47 GLU 0.721310 0.018097 49 GLN 0.776415 0.020360 51 MET 0.714741 0.020147 52 ILE 0.730811 0.020756 55 VAL 0.674105 0.025761 57 ALA 0.688795 0.020426 59 GLY 0.767180 0.018891 60 ASP 0.824747 0.021322 61 GLY 0.762994 0.023760 62 THR 0.820607 0.019701 63 ILE 0.764146 0.032970 64 ASP 0.738430 0.030615 65 PHE 0.806238 0.033538 67 GLU 0.751025 0.028629 69 LEU 0.705218 0.028471 70 THR 0.800866 0.023568 74 ARG 0.758429 0.034331 87 GLU 0.728686 0.027415 89 PHE 0.719738 0.022445 91 VAL 0.750704 0.020838 92 PHE 0.729427 0.025583 93 ASP 0.733875 0.022501 94 LYS 0.680406 0.018013 96 GLY 0.793278 0.018235 97 ASN 0.752265 0.018846 98 GLY 0.781970 0.024754 100 ILE 0.772329 0.027710 102 ALA 0.790814 0.024558 106 ARG 0.765553 0.021450 109 MET 0.789489 0.022528 111 ASN 0.724634 0.022131 112 LEU 0.769583 0.021975 113 GLY 0.678609 0.025054 114 GLU 0.671007 0.021712 115 LYS 0.465153 0.069899 116 LEU 0.384056 0.025631 117 THR 0.572585 0.027423 120 GLU 0.718530 0.018049 123 GLU 0.728528 0.017684 125 ILE 0.740750 0.027341 126 ARG 0.783801 0.023143 127 GLU 0.668666 0.021320 128 ALA 0.738146 0.022434 129 ASP 0.702860 0.019315 130 ILE 0.688957 0.021415 132 GLY 0.735071 0.016883 133 ASP 0.748228 0.018438 134 GLY 0.751583 0.021004 135 GLN 0.717533 0.018428 136 VAL 0.728783 0.023890 137 ASN 0.771554 0.025919 138 TYR 0.787812 0.022284 142 VAL 0.734129 0.020678 143 GLN 0.697193 0.017820 144 MET 0.707971 0.020790 145 MET 0.687279 0.022607 146 THR 0.659069 0.022420 147 ALA 0.514505 0.063662 148 LYS -0.054166 0.018105 stop_ save_ save_heteronuclear_NOE_73C _Saveframe_category heteronuclear_NOE _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_5 _Spectrometer_frequency_1H 500 _Mol_system_component_name Calmodulin _Atom_one_atom_name H _Atom_two_atom_name N _Heteronuclear_NOE_value_type . _NOE_reference_value . _NOE_reference_description . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Heteronuclear_NOE_value _Heteronuclear_NOE_value_error 8 GLN 0.746070 0.029690 9 ILE 0.758880 0.024100 11 GLU 0.789990 0.024530 12 PHE 0.747900 0.026170 15 ALA 0.738540 0.024350 16 PHE 0.758580 0.019750 19 PHE 0.757670 0.026530 21 LYS 0.778650 0.020690 23 GLY 0.771620 0.016250 25 GLY 0.794780 0.022080 27 ILE 0.764800 0.030180 28 THR 0.793060 0.024050 32 LEU 0.737090 0.021240 33 GLY 0.818250 0.024060 39 LEU 0.803320 0.020850 40 GLY 0.837270 0.031440 44 THR 0.765820 0.022580 47 GLU 0.733360 0.016980 49 GLN 0.778890 0.017330 52 ILE 0.738930 0.022370 55 VAL 0.706020 0.023850 57 ALA 0.680590 0.019430 59 GLY 0.817100 0.018690 61 GLY 0.784100 0.021720 62 THR 0.865050 0.020510 63 ILE 0.773960 0.024240 64 ASP 0.779760 0.031000 65 PHE 0.824930 0.036310 67 GLU 0.768370 0.032170 69 LEU 0.745620 0.034120 70 THR 0.795490 0.028840 74 ARG 0.750000 0.074580 89 PHE 0.737140 0.021510 91 VAL 0.797100 0.022350 92 PHE 0.757370 0.026610 93 ASP 0.763120 0.022220 94 LYS 0.664480 0.019660 96 GLY 0.817450 0.018540 97 ASN 0.860210 0.016860 98 GLY 0.754520 0.021180 100 ILE 0.737060 0.027970 102 ALA 0.864190 0.026590 106 ARG 0.730860 0.018520 109 MET 0.759880 0.020940 111 ASN 0.787110 0.024920 112 LEU 0.805600 0.022630 113 GLY 0.725300 0.029850 114 GLU 0.699180 0.023900 116 LEU 0.521890 0.036320 117 THR 0.683250 0.030280 120 GLU 0.719450 0.018560 123 GLU 0.791200 0.018220 125 ILE 0.766530 0.032290 127 GLU 0.670220 0.025140 128 ALA 0.792160 0.026730 129 ASP 0.726280 0.019540 130 ILE 0.702620 0.017760 132 GLY 0.748880 0.016030 133 ASP 0.820230 0.015290 134 GLY 0.756990 0.021250 135 GLN 0.761910 0.017310 136 VAL 0.781510 0.019020 137 ASN 0.820740 0.030080 138 TYR 0.788280 0.019690 142 VAL 0.735450 0.018150 143 GLN 0.739390 0.018390 144 MET 0.697140 0.021350 145 MET 0.674890 0.023800 146 THR 0.706600 0.026150 148 LYS -0.213220 0.027350 stop_ save_ save_S2_parameters_15N_22C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_1 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU N . 0.645 0.006 . . . . . . . . . . . . 5 THR N . 0.822 0.012 . . . . . . . . . . . . 6 GLU N . 0.884 0.013 . . . . . . . . . . . . 7 GLU N . 0.894 0.011 . . . . . . . . . . . . 8 GLN N . 0.880 0.012 . . . . . . . . . . . . 11 GLU N . 0.897 0.012 . . . . . . . . . . . . 13 LYS N . 0.916 0.013 . . . . . . . . . . . . 16 PHE N . 0.927 0.015 . . . . . . . . . . . . 18 LEU N . 0.933 0.013 . . . . . . . . . . . . 19 PHE N . 0.896 0.017 . . . . . . . . . . . . 20 ASP N . 0.874 0.015 . . . . . . . . . . . . 21 LYS N . 0.838 0.012 . . . . . . . . . . . . 23 GLY N . 0.870 0.011 . . . . . . . . . . . . 24 ASP N . 0.899 0.011 . . . . . . . . . . . . 26 THR N . 0.917 0.011 . . . . . . . . . . . . 27 ILE N . 0.867 0.022 . . . . . . . . . . . . 28 THR N . 0.904 0.020 . . . . . . . . . . . . 29 THR N . 0.916 0.013 . . . . . . . . . . . . 31 GLU N . 0.902 0.017 . . . . . . . . . . . . 33 GLY N . 0.913 0.016 . . . . . . . . . . . . 34 THR N . 0.886 0.012 . . . . . . . . . . . . 36 MET N . 0.922 0.015 . . . . . . . . . . . . 37 ARG N . 0.921 0.015 . . . . . . . . . . . . 39 LEU N . 0.888 0.012 . . . . . . . . . . . . 40 GLY N . 0.842 0.011 . . . . . . . . . . . . 42 ASN N . 0.752 0.019 . . . . . . . . . . . . 44 THR N . 0.856 0.015 . . . . . . . . . . . . 45 GLU N . 0.903 0.013 . . . . . . . . . . . . 47 GLU N . 0.890 0.011 . . . . . . . . . . . . 49 GLN N . 0.907 0.011 . . . . . . . . . . . . 52 ILE N . 0.895 0.013 . . . . . . . . . . . . 53 ASN N . 0.909 0.011 . . . . . . . . . . . . 54 GLU N . 0.880 0.009 . . . . . . . . . . . . 55 VAL N . 0.892 0.015 . . . . . . . . . . . . 57 ALA N . 0.667 0.008 . . . . . . . . . . . . 60 ASP N . 0.890 0.009 . . . . . . . . . . . . 62 THR N . 0.905 0.009 . . . . . . . . . . . . 63 ILE N . 0.906 0.020 . . . . . . . . . . . . 64 ASP N . 0.882 0.018 . . . . . . . . . . . . 65 PHE N . 38.322 0.000 . . . . . . . . . . . . 67 GLU N . 17.322 0.000 . . . . . . . . . . . . 69 LEU N . 0.954 0.017 . . . . . . . . . . . . 70 THR N . 0.890 0.012 . . . . . . . . . . . . 71 MET N . 0.921 0.015 . . . . . . . . . . . . 74 ARG N . 0.856 0.014 . . . . . . . . . . . . 75 LYS N . 0.932 0.015 . . . . . . . . . . . . 77 LYS N . 0.857 0.020 . . . . . . . . . . . . 80 ASP N . 0.843 0.021 . . . . . . . . . . . . 81 SER N . 0.913 0.014 . . . . . . . . . . . . 87 GLU N . 0.921 0.013 . . . . . . . . . . . . 89 PHE N . 0.921 0.014 . . . . . . . . . . . . 91 VAL N . 0.893 0.011 . . . . . . . . . . . . 92 PHE N . 0.900 0.016 . . . . . . . . . . . . 93 ASP N . 0.871 0.013 . . . . . . . . . . . . 94 LYS N . 0.848 0.012 . . . . . . . . . . . . 96 GLY N . 0.882 0.012 . . . . . . . . . . . . 100 ILE N . 0.833 0.020 . . . . . . . . . . . . 101 SER N . 0.928 0.022 . . . . . . . . . . . . 102 ALA N . 0.908 0.012 . . . . . . . . . . . . 105 LEU N . 0.934 0.014 . . . . . . . . . . . . 106 ARG N . 0.920 0.013 . . . . . . . . . . . . 109 MET N . 0.929 0.015 . . . . . . . . . . . . 110 THR N . 0.905 0.015 . . . . . . . . . . . . 111 ASN N . 0.905 0.013 . . . . . . . . . . . . 112 LEU N . 0.896 0.012 . . . . . . . . . . . . 113 GLY N . 0.860 0.014 . . . . . . . . . . . . 115 LYS N . 0.707 0.021 . . . . . . . . . . . . 116 LEU N . 0.675 0.007 . . . . . . . . . . . . 117 THR N . 0.828 0.015 . . . . . . . . . . . . 118 ASP N . 0.907 0.017 . . . . . . . . . . . . 120 GLU N . 0.875 0.013 . . . . . . . . . . . . 123 GLU N . 0.897 0.010 . . . . . . . . . . . . 125 ILE N . 0.920 0.016 . . . . . . . . . . . . 126 ARG N . 0.911 0.012 . . . . . . . . . . . . 127 GLU N . 0.899 0.011 . . . . . . . . . . . . 128 ALA N . 0.904 0.013 . . . . . . . . . . . . 129 ASP N . 0.870 0.011 . . . . . . . . . . . . 130 ILE N . 0.635 0.010 . . . . . . . . . . . . 131 ASP N . 0.900 0.011 . . . . . . . . . . . . 133 ASP N . 0.898 0.009 . . . . . . . . . . . . 134 GLY N . 0.892 0.014 . . . . . . . . . . . . 135 GLN N . 0.890 0.009 . . . . . . . . . . . . 136 VAL N . 0.881 0.016 . . . . . . . . . . . . 137 ASN N . 0.881 0.019 . . . . . . . . . . . . 139 GLU N . 0.885 0.009 . . . . . . . . . . . . 143 GLN N . 0.926 0.011 . . . . . . . . . . . . 144 MET N . 0.919 0.013 . . . . . . . . . . . . 145 MET N . 0.894 0.016 . . . . . . . . . . . . 146 THR N . 0.827 0.012 . . . . . . . . . . . . 147 ALA N . 0.593 0.014 . . . . . . . . . . . . 148 LYS N . 0.367 0.011 . . . . . . . . . . . . stop_ save_ save_S2_parameters_15N_35C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_2 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU N . 0.569 0.007 . . . . . . . . . . . . 5 THR N . 0.778 0.010 . . . . . . . . . . . . 6 GLU N . 0.858 0.016 . . . . . . . . . . . . 7 GLU N . 0.893 0.013 . . . . . . . . . . . . 8 GLN N . 0.844 0.011 . . . . . . . . . . . . 9 ILE N . 0.863 0.011 . . . . . . . . . . . . 11 GLU N . 0.876 0.009 . . . . . . . . . . . . 12 PHE N . 0.899 0.010 . . . . . . . . . . . . 13 LYS N . 0.903 0.010 . . . . . . . . . . . . 15 ALA N . 0.886 0.010 . . . . . . . . . . . . 16 PHE N . 0.891 0.012 . . . . . . . . . . . . 18 LEU N . 0.897 0.010 . . . . . . . . . . . . 19 PHE N . 0.866 0.013 . . . . . . . . . . . . 20 ASP N . 0.827 0.012 . . . . . . . . . . . . 21 LYS N . 0.796 0.010 . . . . . . . . . . . . 23 GLY N . 0.845 0.010 . . . . . . . . . . . . 24 ASP N . 0.880 0.010 . . . . . . . . . . . . 26 THR N . 0.897 0.011 . . . . . . . . . . . . 27 ILE N . 0.847 0.017 . . . . . . . . . . . . 28 THR N . 0.883 0.017 . . . . . . . . . . . . 29 THR N . 0.871 0.013 . . . . . . . . . . . . 30 LYS N . 0.886 0.017 . . . . . . . . . . . . 31 GLU N . 0.865 0.013 . . . . . . . . . . . . 32 LEU N . 0.914 0.011 . . . . . . . . . . . . 33 GLY N . 0.877 0.012 . . . . . . . . . . . . 36 MET N . 0.913 0.011 . . . . . . . . . . . . 37 ARG N . 0.902 0.012 . . . . . . . . . . . . 39 LEU N . 0.854 0.010 . . . . . . . . . . . . 40 GLY N . 0.799 0.010 . . . . . . . . . . . . 42 ASN N . 0.707 0.059 . . . . . . . . . . . . 44 THR N . 0.819 0.012 . . . . . . . . . . . . 45 GLU N . 0.905 0.015 . . . . . . . . . . . . 47 GLU N . 0.849 0.010 . . . . . . . . . . . . 49 GLN N . 0.873 0.009 . . . . . . . . . . . . 51 MET N . 0.856 0.009 . . . . . . . . . . . . 52 ILE N . 0.882 0.011 . . . . . . . . . . . . 53 ASN N . 0.885 0.009 . . . . . . . . . . . . 54 GLU N . 0.883 0.007 . . . . . . . . . . . . 55 VAL N . 0.835 0.012 . . . . . . . . . . . . 57 ALA N . 0.639 0.007 . . . . . . . . . . . . 60 ASP N . 0.859 0.009 . . . . . . . . . . . . 62 THR N . 0.878 0.009 . . . . . . . . . . . . 63 ILE N . 0.894 0.015 . . . . . . . . . . . . 64 ASP N . 0.866 0.015 . . . . . . . . . . . . 65 PHE N . 0.943 0.017 . . . . . . . . . . . . 67 GLU N . 0.888 0.018 . . . . . . . . . . . . 68 PHE N . 0.895 0.015 . . . . . . . . . . . . 69 LEU N . 0.903 0.014 . . . . . . . . . . . . 70 THR N . 0.873 0.010 . . . . . . . . . . . . 71 MET N . 0.901 0.012 . . . . . . . . . . . . 74 ARG N . 0.792 0.010 . . . . . . . . . . . . 75 LYS N . 0.839 0.022 . . . . . . . . . . . . 77 LYS N . 0.783 0.031 . . . . . . . . . . . . 80 ASP N . 0.790 0.020 . . . . . . . . . . . . 81 SER N . 0.898 0.025 . . . . . . . . . . . . 85 ILE N . 0.882 0.011 . . . . . . . . . . . . 86 ARG N . 0.887 0.011 . . . . . . . . . . . . 87 GLU N . 0.880 0.011 . . . . . . . . . . . . 89 PHE N . 0.898 0.011 . . . . . . . . . . . . 91 VAL N . 0.878 0.009 . . . . . . . . . . . . 92 PHE N . 0.859 0.013 . . . . . . . . . . . . 93 ASP N . 0.853 0.011 . . . . . . . . . . . . 94 LYS N . 0.834 0.009 . . . . . . . . . . . . 96 GLY N . 0.867 0.011 . . . . . . . . . . . . 97 ASN N . 0.879 0.010 . . . . . . . . . . . . 100 ILE N . 0.833 0.015 . . . . . . . . . . . . 101 SER N . 0.902 0.016 . . . . . . . . . . . . 102 ALA N . 0.870 0.011 . . . . . . . . . . . . 103 ALA N . 0.916 0.011 . . . . . . . . . . . . 105 LEU N . 0.884 0.011 . . . . . . . . . . . . 106 ARG N . 0.911 0.010 . . . . . . . . . . . . 109 MET N . 0.882 0.011 . . . . . . . . . . . . 110 THR N . 0.880 0.012 . . . . . . . . . . . . 111 ASN N . 0.894 0.010 . . . . . . . . . . . . 112 LEU N . 0.849 0.009 . . . . . . . . . . . . 113 GLY N . 0.831 0.011 . . . . . . . . . . . . 114 GLU N . 0.853 0.009 . . . . . . . . . . . . 115 LYS N . 0.652 0.010 . . . . . . . . . . . . 116 LEU N . 0.594 0.006 . . . . . . . . . . . . 117 THR N . 0.780 0.012 . . . . . . . . . . . . 118 ASP N . 0.871 0.029 . . . . . . . . . . . . 119 GLU N . 0.900 0.012 . . . . . . . . . . . . 120 GLU N . 0.862 0.010 . . . . . . . . . . . . 123 GLU N . 0.892 0.009 . . . . . . . . . . . . 125 ILE N . 0.893 0.011 . . . . . . . . . . . . 126 ARG N . 0.905 0.009 . . . . . . . . . . . . 127 GLU N . 0.874 0.009 . . . . . . . . . . . . 128 ALA N . 0.874 0.010 . . . . . . . . . . . . 130 ILE N . 0.610 0.008 . . . . . . . . . . . . 131 ASP N . 0.866 0.010 . . . . . . . . . . . . 133 ASP N . 0.870 0.009 . . . . . . . . . . . . 134 GLY N . 0.877 0.012 . . . . . . . . . . . . 135 GLN N . 0.879 0.008 . . . . . . . . . . . . 136 VAL N . 0.864 0.012 . . . . . . . . . . . . 137 ASN N . 0.870 0.015 . . . . . . . . . . . . 138 TYR N . 0.891 0.012 . . . . . . . . . . . . 139 GLU N . 0.863 0.008 . . . . . . . . . . . . 141 PHE N . 0.904 0.013 . . . . . . . . . . . . 142 VAL N . 0.915 0.010 . . . . . . . . . . . . 143 GLN N . 0.894 0.008 . . . . . . . . . . . . 144 MET N . 0.880 0.010 . . . . . . . . . . . . 145 MET N . 0.860 0.012 . . . . . . . . . . . . 146 THR N . 0.795 0.011 . . . . . . . . . . . . 147 ALA N . 0.765 0.012 . . . . . . . . . . . . 148 LYS N . 0.547 0.003 . . . . . . . . . . . . stop_ save_ save_S2_parameters_15N_47C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_3 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 6 GLU N . 0.819 0.033 . . . . . . . . . . . . 7 GLU N . 0.847 0.018 . . . . . . . . . . . . 8 GLN N . 0.840 0.007 . . . . . . . . . . . . 9 ILE N . 0.845 0.007 . . . . . . . . . . . . 12 PHE N . 0.872 0.007 . . . . . . . . . . . . 13 LYS N . 0.864 0.007 . . . . . . . . . . . . 15 ALA N . 0.850 0.007 . . . . . . . . . . . . 16 PHE N . 0.860 0.009 . . . . . . . . . . . . 18 LEU N . 0.890 0.007 . . . . . . . . . . . . 19 PHE N . 0.838 0.009 . . . . . . . . . . . . 21 LYS N . 0.775 0.006 . . . . . . . . . . . . 23 GLY N . 0.837 0.007 . . . . . . . . . . . . 25 GLY N . 0.849 0.009 . . . . . . . . . . . . 26 THR N . 0.868 0.008 . . . . . . . . . . . . 27 ILE N . 0.834 0.011 . . . . . . . . . . . . 28 THR N . 0.874 0.011 . . . . . . . . . . . . 29 THR N . 0.870 0.015 . . . . . . . . . . . . 30 LYS N . 0.867 0.040 . . . . . . . . . . . . 31 GLU N . 0.856 0.008 . . . . . . . . . . . . 33 GLY N . 0.870 0.009 . . . . . . . . . . . . 34 THR N . 0.842 0.007 . . . . . . . . . . . . 37 ARG N . 0.863 0.007 . . . . . . . . . . . . 39 LEU N . 0.825 0.007 . . . . . . . . . . . . 40 GLY N . 0.803 0.008 . . . . . . . . . . . . 44 THR N . 0.797 0.008 . . . . . . . . . . . . 45 GLU N . 0.831 0.029 . . . . . . . . . . . . 47 GLU N . 0.830 0.007 . . . . . . . . . . . . 49 GLN N . 0.872 0.006 . . . . . . . . . . . . 51 MET N . 0.844 0.007 . . . . . . . . . . . . 52 ILE N . 0.838 0.007 . . . . . . . . . . . . 55 VAL N . 0.830 0.009 . . . . . . . . . . . . 57 ALA N . 0.613 0.005 . . . . . . . . . . . . 59 GLY N . 0.775 0.007 . . . . . . . . . . . . 60 ASP N . 0.846 0.007 . . . . . . . . . . . . 61 GLY N . 0.855 0.009 . . . . . . . . . . . . 62 THR N . 0.879 0.007 . . . . . . . . . . . . 63 ILE N . 0.868 0.011 . . . . . . . . . . . . 64 ASP N . 0.864 0.010 . . . . . . . . . . . . 65 PHE N . 0.913 0.012 . . . . . . . . . . . . 67 GLU N . 0.871 0.011 . . . . . . . . . . . . 69 LEU N . 0.888 0.009 . . . . . . . . . . . . 70 THR N . 0.848 0.006 . . . . . . . . . . . . 71 MET N . 0.872 0.008 . . . . . . . . . . . . 85 ILE N . 0.867 0.008 . . . . . . . . . . . . 87 GLU N . 0.885 0.007 . . . . . . . . . . . . 89 PHE N . 0.889 0.007 . . . . . . . . . . . . 91 VAL N . 0.845 0.007 . . . . . . . . . . . . 92 PHE N . 0.839 0.008 . . . . . . . . . . . . 93 ASP N . 0.841 0.007 . . . . . . . . . . . . 94 LYS N . 0.807 0.006 . . . . . . . . . . . . 96 GLY N . 0.861 0.007 . . . . . . . . . . . . 97 ASN N . 0.854 0.008 . . . . . . . . . . . . 98 GLY N . 0.869 0.010 . . . . . . . . . . . . 100 ILE N . 0.823 0.011 . . . . . . . . . . . . 101 SER N . 0.875 0.011 . . . . . . . . . . . . 102 ALA N . 0.851 0.008 . . . . . . . . . . . . 106 ARG N . 0.884 0.006 . . . . . . . . . . . . 109 MET N . 0.881 0.006 . . . . . . . . . . . . 111 ASN N . 0.875 0.007 . . . . . . . . . . . . 112 LEU N . 0.825 0.007 . . . . . . . . . . . . 113 GLY N . 0.801 0.008 . . . . . . . . . . . . 114 GLU N . 0.830 0.007 . . . . . . . . . . . . 119 GLU N . 0.861 0.015 . . . . . . . . . . . . 120 GLU N . 0.842 0.007 . . . . . . . . . . . . 123 GLU N . 0.877 0.006 . . . . . . . . . . . . 125 ILE N . 0.891 0.007 . . . . . . . . . . . . 126 ARG N . 0.872 0.006 . . . . . . . . . . . . 127 GLU N . 0.857 0.006 . . . . . . . . . . . . 128 ALA N . 0.855 0.007 . . . . . . . . . . . . 129 ASP N . 0.853 0.007 . . . . . . . . . . . . 130 ILE N . 0.610 0.006 . . . . . . . . . . . . 132 GLY N . 0.776 0.006 . . . . . . . . . . . . 133 ASP N . 0.852 0.006 . . . . . . . . . . . . 134 GLY N . 0.866 0.008 . . . . . . . . . . . . 135 GLN N . 0.837 0.007 . . . . . . . . . . . . 136 VAL N . 0.831 0.009 . . . . . . . . . . . . 137 ASN N . 0.853 0.011 . . . . . . . . . . . . 138 TYR N . 0.871 0.008 . . . . . . . . . . . . 142 VAL N . 0.888 0.007 . . . . . . . . . . . . 143 GLN N . 0.884 0.006 . . . . . . . . . . . . 144 MET N . 0.839 0.007 . . . . . . . . . . . . 145 MET N . 0.834 0.008 . . . . . . . . . . . . 146 THR N . 0.780 0.009 . . . . . . . . . . . . stop_ save_ save_S2_parameters_15N_60C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_4 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU N . 0.410 0.024 . . . . . . . . . . . . 5 THR N . 0.722 0.012 . . . . . . . . . . . . 7 GLU N . 0.834 0.042 . . . . . . . . . . . . 8 GLN N . 0.814 0.010 . . . . . . . . . . . . 9 ILE N . 0.840 0.008 . . . . . . . . . . . . 11 GLU N . 0.835 0.006 . . . . . . . . . . . . 12 PHE N . 0.855 0.008 . . . . . . . . . . . . 15 ALA N . 0.845 0.007 . . . . . . . . . . . . 16 PHE N . 0.882 0.008 . . . . . . . . . . . . 19 PHE N . 0.825 0.009 . . . . . . . . . . . . 21 LYS N . 0.755 0.006 . . . . . . . . . . . . 23 GLY N . 0.814 0.007 . . . . . . . . . . . . 25 GLY N . 0.876 0.009 . . . . . . . . . . . . 26 THR N . 0.870 0.007 . . . . . . . . . . . . 27 ILE N . 0.842 0.011 . . . . . . . . . . . . 28 THR N . 0.865 0.010 . . . . . . . . . . . . 29 THR N . 0.859 0.040 . . . . . . . . . . . . 31 GLU N . 0.857 0.008 . . . . . . . . . . . . 32 LEU N . 0.883 0.007 . . . . . . . . . . . . 33 GLY N . 0.883 0.008 . . . . . . . . . . . . 37 ARG N . 0.867 0.007 . . . . . . . . . . . . 39 LEU N . 0.807 0.006 . . . . . . . . . . . . 40 GLY N . 0.792 0.010 . . . . . . . . . . . . 44 THR N . 0.766 0.008 . . . . . . . . . . . . 47 GLU N . 0.828 0.006 . . . . . . . . . . . . 49 GLN N . 0.831 0.006 . . . . . . . . . . . . 51 MET N . 0.844 0.006 . . . . . . . . . . . . 52 ILE N . 0.851 0.007 . . . . . . . . . . . . 55 VAL N . 0.815 0.009 . . . . . . . . . . . . 57 ALA N . 0.608 0.005 . . . . . . . . . . . . 59 GLY N . 0.781 0.007 . . . . . . . . . . . . 60 ASP N . 0.840 0.007 . . . . . . . . . . . . 61 GLY N . 0.834 0.009 . . . . . . . . . . . . 62 THR N . 0.864 0.007 . . . . . . . . . . . . 63 ILE N . 0.866 0.010 . . . . . . . . . . . . 64 ASP N . 0.845 0.011 . . . . . . . . . . . . 65 PHE N . 0.887 0.014 . . . . . . . . . . . . 67 GLU N . 0.867 0.009 . . . . . . . . . . . . 69 LEU N . 0.883 0.010 . . . . . . . . . . . . 70 THR N . 0.862 0.007 . . . . . . . . . . . . 74 ARG N . 0.760 0.011 . . . . . . . . . . . . 87 GLU N . 0.893 0.008 . . . . . . . . . . . . 89 PHE N . 0.863 0.008 . . . . . . . . . . . . 91 VAL N . 0.852 0.006 . . . . . . . . . . . . 92 PHE N . 0.834 0.008 . . . . . . . . . . . . 93 ASP N . 0.833 0.007 . . . . . . . . . . . . 94 LYS N . 0.787 0.006 . . . . . . . . . . . . 96 GLY N . 0.843 0.006 . . . . . . . . . . . . 97 ASN N . 0.850 0.007 . . . . . . . . . . . . 98 GLY N . 0.869 0.010 . . . . . . . . . . . . 100 ILE N . 0.833 0.010 . . . . . . . . . . . . 102 ALA N . 0.875 0.010 . . . . . . . . . . . . 106 ARG N . 0.894 0.006 . . . . . . . . . . . . 109 MET N . 0.885 0.006 . . . . . . . . . . . . 111 ASN N . 0.864 0.008 . . . . . . . . . . . . 112 LEU N . 0.803 0.006 . . . . . . . . . . . . 113 GLY N . 0.792 0.009 . . . . . . . . . . . . 114 GLU N . 0.803 0.007 . . . . . . . . . . . . 115 LYS N . 0.523 0.035 . . . . . . . . . . . . 116 LEU N . 0.508 0.008 . . . . . . . . . . . . 117 THR N . 0.720 0.008 . . . . . . . . . . . . 120 GLU N . 0.829 0.006 . . . . . . . . . . . . 123 GLU N . 0.860 0.006 . . . . . . . . . . . . 125 ILE N . 0.883 0.008 . . . . . . . . . . . . 126 ARG N . 0.903 0.006 . . . . . . . . . . . . 127 GLU N . 0.847 0.006 . . . . . . . . . . . . 128 ALA N . 0.857 0.007 . . . . . . . . . . . . 129 ASP N . 0.828 0.006 . . . . . . . . . . . . 130 ILE N . 0.609 0.005 . . . . . . . . . . . . 132 GLY N . 0.776 0.006 . . . . . . . . . . . . 133 ASP N . 0.843 0.006 . . . . . . . . . . . . 134 GLY N . 0.846 0.008 . . . . . . . . . . . . 135 GLN N . 0.845 0.007 . . . . . . . . . . . . 136 VAL N . 0.829 0.008 . . . . . . . . . . . . 137 ASN N . 0.849 0.009 . . . . . . . . . . . . 138 TYR N . 0.864 0.008 . . . . . . . . . . . . 142 VAL N . 0.866 0.007 . . . . . . . . . . . . 143 GLN N . 0.872 0.006 . . . . . . . . . . . . 144 MET N . 0.835 0.007 . . . . . . . . . . . . 145 MET N . 0.821 0.007 . . . . . . . . . . . . 146 THR N . 0.757 0.009 . . . . . . . . . . . . 147 ALA N . 0.602 0.042 . . . . . . . . . . . . 148 LYS N . 0.381 0.003 . . . . . . . . . . . . stop_ save_ save_S2_parameters_15N_73C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_5 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 8 GLN N . 0.820 0.022 . . . . . . . . . . . . 9 ILE N . 0.884 0.013 . . . . . . . . . . . . 11 GLU N . 0.888 0.012 . . . . . . . . . . . . 12 PHE N . 0.887 0.013 . . . . . . . . . . . . 15 ALA N . 0.886 0.011 . . . . . . . . . . . . 16 PHE N . 0.874 0.010 . . . . . . . . . . . . 19 PHE N . 0.841 0.011 . . . . . . . . . . . . 21 LYS N . 0.750 0.008 . . . . . . . . . . . . 23 GLY N . 0.835 0.009 . . . . . . . . . . . . 25 GLY N . 0.882 0.012 . . . . . . . . . . . . 27 ILE N . 0.845 0.014 . . . . . . . . . . . . 28 THR N . 0.867 0.011 . . . . . . . . . . . . 32 LEU N . 0.872 0.009 . . . . . . . . . . . . 33 GLY N . 0.893 0.010 . . . . . . . . . . . . 39 LEU N . 0.836 0.009 . . . . . . . . . . . . 40 GLY N . 0.830 0.019 . . . . . . . . . . . . 44 THR N . 0.788 0.012 . . . . . . . . . . . . 47 GLU N . 0.828 0.008 . . . . . . . . . . . . 49 GLN N . 0.871 0.007 . . . . . . . . . . . . 52 ILE N . 0.870 0.009 . . . . . . . . . . . . 55 VAL N . 0.839 0.011 . . . . . . . . . . . . 57 ALA N . 0.631 0.008 . . . . . . . . . . . . 59 GLY N . 0.830 0.011 . . . . . . . . . . . . 61 GLY N . 0.881 0.012 . . . . . . . . . . . . 62 THR N . 0.887 0.009 . . . . . . . . . . . . 63 ILE N . 0.860 0.011 . . . . . . . . . . . . 64 ASP N . 0.890 0.015 . . . . . . . . . . . . 65 PHE N . 0.899 0.029 . . . . . . . . . . . . 67 GLU N . 0.892 0.015 . . . . . . . . . . . . 69 LEU N . 0.906 0.015 . . . . . . . . . . . . 70 THR N . 0.883 0.011 . . . . . . . . . . . . 74 ARG N . 0.840 0.045 . . . . . . . . . . . . 89 PHE N . 0.883 0.011 . . . . . . . . . . . . 91 VAL N . 0.864 0.008 . . . . . . . . . . . . 92 PHE N . 0.858 0.012 . . . . . . . . . . . . 93 ASP N . 0.841 0.010 . . . . . . . . . . . . 94 LYS N . 0.764 0.008 . . . . . . . . . . . . 96 GLY N . 0.855 0.009 . . . . . . . . . . . . 97 ASN N . 0.876 0.009 . . . . . . . . . . . . 98 GLY N . 0.897 0.014 . . . . . . . . . . . . 100 ILE N . 0.830 0.015 . . . . . . . . . . . . 102 ALA N . 0.862 0.025 . . . . . . . . . . . . 106 ARG N . 0.847 0.008 . . . . . . . . . . . . 109 MET N . 0.858 0.009 . . . . . . . . . . . . 111 ASN N . 0.896 0.012 . . . . . . . . . . . . 112 LEU N . 0.847 0.009 . . . . . . . . . . . . 113 GLY N . 0.804 0.016 . . . . . . . . . . . . 114 GLU N . 0.824 0.013 . . . . . . . . . . . . 116 LEU N . 0.504 0.019 . . . . . . . . . . . . 117 THR N . 0.746 0.014 . . . . . . . . . . . . 120 GLU N . 0.848 0.009 . . . . . . . . . . . . 123 GLU N . 0.880 0.007 . . . . . . . . . . . . 125 ILE N . 0.887 0.012 . . . . . . . . . . . . 127 GLU N . 0.875 0.011 . . . . . . . . . . . . 128 ALA N . 0.880 0.011 . . . . . . . . . . . . 129 ASP N . 0.837 0.009 . . . . . . . . . . . . 130 ILE N . 0.628 0.007 . . . . . . . . . . . . 132 GLY N . 0.789 0.009 . . . . . . . . . . . . 133 ASP N . 0.868 0.008 . . . . . . . . . . . . 134 GLY N . 0.853 0.012 . . . . . . . . . . . . 135 GLN N . 0.879 0.008 . . . . . . . . . . . . 136 VAL N . 0.864 0.009 . . . . . . . . . . . . 137 ASN N . 0.881 0.014 . . . . . . . . . . . . 138 TYR N . 0.897 0.011 . . . . . . . . . . . . 142 VAL N . 0.875 0.009 . . . . . . . . . . . . 143 GLN N . 0.859 0.008 . . . . . . . . . . . . 144 MET N . 0.851 0.010 . . . . . . . . . . . . 145 MET N . 0.813 0.011 . . . . . . . . . . . . 146 THR N . 0.813 0.015 . . . . . . . . . . . . 148 LYS N . 0.378 0.007 . . . . . . . . . . . . stop_ save_ save_S2_parameters_2H_15C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_6 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU HD1 . 0.365 0.035 . . . . . . . . . . . . 9 ILE HD1 . 0.519 0.014 . . . . . . . . . . . . 10 ALA HB . 0.872 0.041 . . . . . . . . . . . . 15 ALA HB . 0.637 0.132 . . . . . . . . . . . . 18 LEU HD2 . 0.476 0.032 . . . . . . . . . . . . 26 THR HG2 . 0.700 0.062 . . . . . . . . . . . . 27 ILE HD1 . 0.714 0.087 . . . . . . . . . . . . 27 ILE HG2 . 0.670 0.050 . . . . . . . . . . . . 29 THR HG2 . 0.634 0.039 . . . . . . . . . . . . 34 THR HG2 . 0.662 0.032 . . . . . . . . . . . . 35 VAL HG1 . 0.669 0.044 . . . . . . . . . . . . 35 VAL HG2 . 0.634 0.029 . . . . . . . . . . . . 36 MET HE . 0.307 0.003 . . . . . . . . . . . . 39 LEU HD1 . 0.458 0.059 . . . . . . . . . . . . 39 LEU HD2 . 0.575 0.034 . . . . . . . . . . . . 46 ALA HB . 0.743 0.031 . . . . . . . . . . . . 51 MET HE . 0.287 0.003 . . . . . . . . . . . . 52 ILE HD1 . 0.340 0.010 . . . . . . . . . . . . 52 ILE HG2 . 0.775 0.051 . . . . . . . . . . . . 55 VAL HG1 . 0.474 0.018 . . . . . . . . . . . . 55 VAL HG2 . 0.461 0.019 . . . . . . . . . . . . 63 ILE HD1 . 0.739 0.050 . . . . . . . . . . . . 63 ILE HG2 . 0.747 0.053 . . . . . . . . . . . . 69 LEU HD1 . 0.359 0.047 . . . . . . . . . . . . 70 THR HG2 . 0.709 0.031 . . . . . . . . . . . . 71 MET HE . 0.498 0.011 . . . . . . . . . . . . 72 MET HE . 0.756 0.050 . . . . . . . . . . . . 73 ALA HB . 0.746 0.041 . . . . . . . . . . . . 76 MET HE . 0.109 0.002 . . . . . . . . . . . . 79 THR HG2 . 0.399 0.010 . . . . . . . . . . . . 85 ILE HD1 . 0.306 0.008 . . . . . . . . . . . . 85 ILE HG2 . 0.666 0.032 . . . . . . . . . . . . 88 ALA HB . 0.657 0.097 . . . . . . . . . . . . 91 VAL HG1 . 0.790 0.058 . . . . . . . . . . . . 100 ILE HD1 . 0.820 0.105 . . . . . . . . . . . . 102 ALA HB . 0.881 0.052 . . . . . . . . . . . . 103 ALA HB . 0.814 0.035 . . . . . . . . . . . . 105 LEU HD1 . 0.588 0.076 . . . . . . . . . . . . 108 VAL HG1 . 0.495 0.020 . . . . . . . . . . . . 108 VAL HG2 . 0.479 0.014 . . . . . . . . . . . . 109 MET HE . 0.372 0.005 . . . . . . . . . . . . 110 THR HG2 . 0.650 0.023 . . . . . . . . . . . . 112 LEU HD2 . 0.632 0.052 . . . . . . . . . . . . 116 LEU HD1 . 0.195 0.016 . . . . . . . . . . . . 121 VAL HG1 . 0.686 0.028 . . . . . . . . . . . . 124 MET HE . 0.803 0.094 . . . . . . . . . . . . 125 ILE HD1 . 0.454 0.015 . . . . . . . . . . . . 125 ILE HG2 . 0.750 0.041 . . . . . . . . . . . . 128 ALA HB . 0.819 0.127 . . . . . . . . . . . . 130 ILE HD1 . 0.345 0.005 . . . . . . . . . . . . 130 ILE HG2 . 0.595 0.013 . . . . . . . . . . . . 136 VAL HG1 . 0.748 0.099 . . . . . . . . . . . . 142 VAL HG1 . 0.649 0.030 . . . . . . . . . . . . 142 VAL HG2 . 0.633 0.023 . . . . . . . . . . . . 144 MET HE . 0.445 0.019 . . . . . . . . . . . . 145 MET HE . 0.277 0.005 . . . . . . . . . . . . 146 THR HG2 . 0.523 0.014 . . . . . . . . . . . . 147 ALA HB . 0.394 0.006 . . . . . . . . . . . . stop_ save_ save_S2_parameters_2H_22C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_1 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU HD1 . 0.372 0.028 . . . . . . . . . . . . 9 ILE HD1 . 0.542 0.013 . . . . . . . . . . . . 10 ALA HB . 0.836 0.035 . . . . . . . . . . . . 15 ALA HB . 0.684 0.087 . . . . . . . . . . . . 18 LEU HD2 . 0.436 0.023 . . . . . . . . . . . . 26 THR HG2 . 0.641 0.047 . . . . . . . . . . . . 27 ILE HD1 . 0.774 0.072 . . . . . . . . . . . . 27 ILE HG2 . 0.732 0.047 . . . . . . . . . . . . 29 THR HG2 . 0.602 0.032 . . . . . . . . . . . . 34 THR HG2 . 0.676 0.029 . . . . . . . . . . . . 35 VAL HG1 . 0.663 0.036 . . . . . . . . . . . . 35 VAL HG2 . 0.639 0.024 . . . . . . . . . . . . 36 MET HE . 0.318 0.003 . . . . . . . . . . . . 39 LEU HD1 . 0.515 0.051 . . . . . . . . . . . . 39 LEU HD2 . 0.532 0.030 . . . . . . . . . . . . 46 ALA HB . 0.840 0.031 . . . . . . . . . . . . 51 MET HE . 0.293 0.003 . . . . . . . . . . . . 52 ILE HD1 . 0.341 0.009 . . . . . . . . . . . . 52 ILE HG2 . 0.777 0.041 . . . . . . . . . . . . 55 VAL HG1 . 0.476 0.015 . . . . . . . . . . . . 55 VAL HG2 . 0.438 0.016 . . . . . . . . . . . . 63 ILE HD1 . 0.767 0.042 . . . . . . . . . . . . 63 ILE HG2 . 0.764 0.044 . . . . . . . . . . . . 69 LEU HD1 . 0.326 0.035 . . . . . . . . . . . . 70 THR HG2 . 0.663 0.025 . . . . . . . . . . . . 71 MET HE . 0.457 0.007 . . . . . . . . . . . . 72 MET HE . 0.769 0.032 . . . . . . . . . . . . 73 ALA HB . 0.806 0.034 . . . . . . . . . . . . 76 MET HE . 0.112 0.002 . . . . . . . . . . . . 79 THR HG2 . 0.407 0.009 . . . . . . . . . . . . 85 ILE HD1 . 0.303 0.008 . . . . . . . . . . . . 85 ILE HG2 . 0.665 0.026 . . . . . . . . . . . . 88 ALA HB . 0.802 0.085 . . . . . . . . . . . . 91 VAL HG1 . 0.739 0.042 . . . . . . . . . . . . 100 ILE HD1 . 0.823 0.078 . . . . . . . . . . . . 102 ALA HB . 0.914 0.045 . . . . . . . . . . . . 103 ALA HB . 0.868 0.037 . . . . . . . . . . . . 105 LEU HD1 . 0.627 0.058 . . . . . . . . . . . . 108 VAL HG1 . 0.478 0.018 . . . . . . . . . . . . 108 VAL HG2 . 0.449 0.013 . . . . . . . . . . . . 109 MET HE . 0.355 0.004 . . . . . . . . . . . . 110 THR HG2 . 0.659 0.022 . . . . . . . . . . . . 112 LEU HD2 . 0.675 0.046 . . . . . . . . . . . . 116 LEU HD1 . 0.213 0.015 . . . . . . . . . . . . 121 VAL HG1 . 0.625 0.023 . . . . . . . . . . . . 124 MET HE . 0.874 0.048 . . . . . . . . . . . . 125 ILE HD1 . 0.457 0.013 . . . . . . . . . . . . 125 ILE HG2 . 0.810 0.037 . . . . . . . . . . . . 128 ALA HB . 0.906 0.091 . . . . . . . . . . . . 130 ILE HD1 . 0.354 0.006 . . . . . . . . . . . . 130 ILE HG2 . 0.590 0.012 . . . . . . . . . . . . 136 VAL HG1 . 0.746 0.069 . . . . . . . . . . . . 142 VAL HG1 . 0.628 0.025 . . . . . . . . . . . . 142 VAL HG2 . 0.615 0.021 . . . . . . . . . . . . 144 MET HE . 0.415 0.010 . . . . . . . . . . . . 145 MET HE . 0.294 0.005 . . . . . . . . . . . . 146 THR HG2 . 0.532 0.014 . . . . . . . . . . . . 147 ALA HB . 0.382 0.006 . . . . . . . . . . . . stop_ save_ save_S2_parameters_2H_28C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_7 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU HD1 . 0.380 0.029 . . . . . . . . . . . . 9 ILE HD1 . 0.529 0.013 . . . . . . . . . . . . 10 ALA HB . 0.888 0.031 . . . . . . . . . . . . 15 ALA HB . 0.908 0.087 . . . . . . . . . . . . 18 LEU HD2 . 0.435 0.023 . . . . . . . . . . . . 26 THR HG2 . 0.658 0.040 . . . . . . . . . . . . 27 ILE HD1 . 0.816 0.065 . . . . . . . . . . . . 27 ILE HG2 . 0.696 0.037 . . . . . . . . . . . . 29 THR HG2 . 0.588 0.029 . . . . . . . . . . . . 34 THR HG2 . 0.690 0.026 . . . . . . . . . . . . 35 VAL HG1 . 0.707 0.033 . . . . . . . . . . . . 35 VAL HG2 . 0.628 0.023 . . . . . . . . . . . . 36 MET HE . 0.311 0.003 . . . . . . . . . . . . 39 LEU HD1 . 0.550 0.050 . . . . . . . . . . . . 39 LEU HD2 . 0.559 0.031 . . . . . . . . . . . . 46 ALA HB . 0.823 0.027 . . . . . . . . . . . . 51 MET HE . 0.275 0.003 . . . . . . . . . . . . 52 ILE HD1 . 0.340 0.010 . . . . . . . . . . . . 52 ILE HG2 . 0.780 0.034 . . . . . . . . . . . . 55 VAL HG1 . 0.454 0.015 . . . . . . . . . . . . 55 VAL HG2 . 0.412 0.016 . . . . . . . . . . . . 63 ILE HD1 . 0.786 0.035 . . . . . . . . . . . . 63 ILE HG2 . 0.809 0.039 . . . . . . . . . . . . 69 LEU HD1 . 0.323 0.048 . . . . . . . . . . . . 70 THR HG2 . 0.693 0.023 . . . . . . . . . . . . 71 MET HE . 0.450 0.007 . . . . . . . . . . . . 72 MET HE . 0.717 0.023 . . . . . . . . . . . . 73 ALA HB . 0.814 0.033 . . . . . . . . . . . . 76 MET HE . 0.103 0.002 . . . . . . . . . . . . 79 THR HG2 . 0.425 0.010 . . . . . . . . . . . . 85 ILE HD1 . 0.321 0.009 . . . . . . . . . . . . 85 ILE HG2 . 0.656 0.023 . . . . . . . . . . . . 88 ALA HB . 0.818 0.086 . . . . . . . . . . . . 91 VAL HG1 . 0.757 0.038 . . . . . . . . . . . . 100 ILE HD1 . 0.812 0.062 . . . . . . . . . . . . 102 ALA HB . 0.963 0.038 . . . . . . . . . . . . 103 ALA HB . 0.861 0.030 . . . . . . . . . . . . 105 LEU HD1 . 0.600 0.051 . . . . . . . . . . . . 108 VAL HG1 . 0.505 0.017 . . . . . . . . . . . . 108 VAL HG2 . 0.458 0.013 . . . . . . . . . . . . 109 MET HE . 0.364 0.005 . . . . . . . . . . . . 110 THR HG2 . 0.660 0.019 . . . . . . . . . . . . 112 LEU HD2 . 0.639 0.043 . . . . . . . . . . . . 116 LEU HD1 . 0.181 0.019 . . . . . . . . . . . . 121 VAL HG1 . 0.673 0.021 . . . . . . . . . . . . 124 MET HE . 0.879 0.032 . . . . . . . . . . . . 125 ILE HD1 . 0.454 0.013 . . . . . . . . . . . . 125 ILE HG2 . 0.837 0.032 . . . . . . . . . . . . 128 ALA HB . 0.848 0.077 . . . . . . . . . . . . 130 ILE HD1 . 0.359 0.007 . . . . . . . . . . . . 130 ILE HG2 . 0.614 0.011 . . . . . . . . . . . . 136 VAL HG1 . 0.779 0.061 . . . . . . . . . . . . 142 VAL HG1 . 0.630 0.022 . . . . . . . . . . . . 142 VAL HG2 . 0.638 0.018 . . . . . . . . . . . . 144 MET HE . 0.398 0.009 . . . . . . . . . . . . 145 MET HE . 0.292 0.005 . . . . . . . . . . . . 146 THR HG2 . 0.538 0.014 . . . . . . . . . . . . 147 ALA HB . 0.386 0.006 . . . . . . . . . . . . stop_ save_ save_S2_parameters_2H_35C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_2 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU HD1 . 0.333 0.018 . . . . . . . . . . . . 9 ILE HD1 . 0.514 0.010 . . . . . . . . . . . . 10 ALA HB . 0.879 0.021 . . . . . . . . . . . . 15 ALA HB . 0.880 0.041 . . . . . . . . . . . . 18 LEU HD2 . 0.381 0.014 . . . . . . . . . . . . 26 THR HG2 . 0.616 0.023 . . . . . . . . . . . . 27 ILE HD1 . 0.765 0.038 . . . . . . . . . . . . 27 ILE HG2 . 0.804 0.027 . . . . . . . . . . . . 29 THR HG2 . 0.573 0.018 . . . . . . . . . . . . 34 THR HG2 . 0.632 0.017 . . . . . . . . . . . . 35 VAL HG1 . 0.684 0.020 . . . . . . . . . . . . 35 VAL HG2 . 0.632 0.014 . . . . . . . . . . . . 36 MET HE . 0.294 0.003 . . . . . . . . . . . . 39 LEU HD1 . 0.468 0.031 . . . . . . . . . . . . 39 LEU HD2 . 0.530 0.019 . . . . . . . . . . . . 46 ALA HB . 0.830 0.018 . . . . . . . . . . . . 51 MET HE . 0.261 0.003 . . . . . . . . . . . . 52 ILE HD1 . 0.323 0.007 . . . . . . . . . . . . 52 ILE HG2 . 0.771 0.022 . . . . . . . . . . . . 55 VAL HG1 . 0.440 0.010 . . . . . . . . . . . . 55 VAL HG2 . 0.408 0.011 . . . . . . . . . . . . 63 ILE HD1 . 0.748 0.023 . . . . . . . . . . . . 63 ILE HG2 . 0.767 0.023 . . . . . . . . . . . . 69 LEU HD1 . 0.335 0.030 . . . . . . . . . . . . 70 THR HG2 . 0.660 0.015 . . . . . . . . . . . . 71 MET HE . 0.398 0.005 . . . . . . . . . . . . 72 MET HE . 0.719 0.013 . . . . . . . . . . . . 73 ALA HB . 0.793 0.019 . . . . . . . . . . . . 76 MET HE . 0.097 0.002 . . . . . . . . . . . . 79 THR HG2 . 0.379 0.007 . . . . . . . . . . . . 85 ILE HD1 . 0.311 0.007 . . . . . . . . . . . . 85 ILE HG2 . 0.630 0.015 . . . . . . . . . . . . 88 ALA HB . 0.875 0.043 . . . . . . . . . . . . 91 VAL HG1 . 0.742 0.023 . . . . . . . . . . . . 100 ILE HD1 . 0.867 0.041 . . . . . . . . . . . . 102 ALA HB . 0.919 0.025 . . . . . . . . . . . . 103 ALA HB . 0.869 0.021 . . . . . . . . . . . . 105 LEU HD1 . 0.566 0.029 . . . . . . . . . . . . 108 VAL HG1 . 0.498 0.013 . . . . . . . . . . . . 108 VAL HG2 . 0.450 0.009 . . . . . . . . . . . . 109 MET HE . 0.332 0.003 . . . . . . . . . . . . 110 THR HG2 . 0.655 0.014 . . . . . . . . . . . . 112 LEU HD2 . 0.595 0.026 . . . . . . . . . . . . 116 LEU HD1 . 0.189 0.014 . . . . . . . . . . . . 121 VAL HG1 . 0.628 0.014 . . . . . . . . . . . . 124 MET HE . 0.839 0.018 . . . . . . . . . . . . 125 ILE HD1 . 0.405 0.008 . . . . . . . . . . . . 125 ILE HG2 . 0.828 0.021 . . . . . . . . . . . . 128 ALA HB . 0.932 0.044 . . . . . . . . . . . . 130 ILE HD1 . 0.356 0.006 . . . . . . . . . . . . 130 ILE HG2 . 0.570 0.008 . . . . . . . . . . . . 136 VAL HG1 . 0.825 0.037 . . . . . . . . . . . . 142 VAL HG1 . 0.635 0.015 . . . . . . . . . . . . 142 VAL HG2 . 0.585 0.013 . . . . . . . . . . . . 144 MET HE . 0.377 0.006 . . . . . . . . . . . . 145 MET HE . 0.285 0.004 . . . . . . . . . . . . 146 THR HG2 . 0.495 0.010 . . . . . . . . . . . . 147 ALA HB . 0.365 0.005 . . . . . . . . . . . . stop_ save_ save_S2_parameters_2H_38C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_8 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU HD1 . 0.277 0.018 . . . . . . . . . . . . 9 ILE HD1 . 0.510 0.011 . . . . . . . . . . . . 10 ALA HB . 0.825 0.019 . . . . . . . . . . . . 15 ALA HB . 0.868 0.051 . . . . . . . . . . . . 18 LEU HD2 . 0.384 0.016 . . . . . . . . . . . . 26 THR HG2 . 0.619 0.022 . . . . . . . . . . . . 27 ILE HD1 . 0.839 0.046 . . . . . . . . . . . . 27 ILE HG2 . 0.699 0.025 . . . . . . . . . . . . 29 THR HG2 . 0.548 0.018 . . . . . . . . . . . . 34 THR HG2 . 0.633 0.017 . . . . . . . . . . . . 35 VAL HG1 . 0.677 0.021 . . . . . . . . . . . . 35 VAL HG2 . 0.631 0.016 . . . . . . . . . . . . 39 LEU HD1 . 0.482 0.034 . . . . . . . . . . . . 39 LEU HD2 . 0.537 0.022 . . . . . . . . . . . . 46 ALA HB . 0.787 0.018 . . . . . . . . . . . . 52 ILE HD1 . 0.314 0.007 . . . . . . . . . . . . 52 ILE HG2 . 0.767 0.022 . . . . . . . . . . . . 55 VAL HG1 . 0.434 0.010 . . . . . . . . . . . . 55 VAL HG2 . 0.394 0.012 . . . . . . . . . . . . 63 ILE HD1 . 0.740 0.026 . . . . . . . . . . . . 63 ILE HG2 . 0.777 0.025 . . . . . . . . . . . . 69 LEU HD1 . 0.286 0.035 . . . . . . . . . . . . 70 THR HG2 . 0.593 0.014 . . . . . . . . . . . . 71 MET HE . 0.365 0.005 . . . . . . . . . . . . 72 MET HE . 0.682 0.015 . . . . . . . . . . . . 73 ALA HB . 0.827 0.023 . . . . . . . . . . . . 76 MET HE . 0.082 0.002 . . . . . . . . . . . . 79 THR HG2 . 0.345 0.008 . . . . . . . . . . . . 85 ILE HD1 . 0.296 0.008 . . . . . . . . . . . . 85 ILE HG2 . 0.641 0.016 . . . . . . . . . . . . 88 ALA HB . 0.795 0.041 . . . . . . . . . . . . 100 ILE HD1 . 0.850 0.044 . . . . . . . . . . . . 102 ALA HB . 0.906 0.025 . . . . . . . . . . . . 103 ALA HB . 0.842 0.022 . . . . . . . . . . . . 105 LEU HD1 . 0.565 0.030 . . . . . . . . . . . . 108 VAL HG1 . 0.485 0.013 . . . . . . . . . . . . 108 VAL HG2 . 0.437 0.009 . . . . . . . . . . . . 109 MET HE . 0.305 0.004 . . . . . . . . . . . . 110 THR HG2 . 0.627 0.014 . . . . . . . . . . . . 112 LEU HD2 . 0.595 0.028 . . . . . . . . . . . . 116 LEU HD1 . 0.170 0.014 . . . . . . . . . . . . 121 VAL HG1 . 0.623 0.014 . . . . . . . . . . . . 124 MET HE . 0.803 0.022 . . . . . . . . . . . . 125 ILE HD1 . 0.386 0.009 . . . . . . . . . . . . 125 ILE HG2 . 0.814 0.022 . . . . . . . . . . . . 128 ALA HB . 0.975 0.048 . . . . . . . . . . . . 130 ILE HD1 . 0.339 0.006 . . . . . . . . . . . . 130 ILE HG2 . 0.554 0.009 . . . . . . . . . . . . 136 VAL HG1 . 0.804 0.037 . . . . . . . . . . . . 142 VAL HG2 . 0.582 0.012 . . . . . . . . . . . . 144 MET HE . 0.344 0.007 . . . . . . . . . . . . 145 MET HE . 0.264 0.004 . . . . . . . . . . . . 146 THR HG2 . 0.515 0.010 . . . . . . . . . . . . 147 ALA HB . 0.345 0.005 . . . . . . . . . . . . stop_ save_ save_S2_parameters_2H_41C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_9 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU HD1 . 0.297 0.020 . . . . . . . . . . . . 9 ILE HD1 . 0.486 0.011 . . . . . . . . . . . . 10 ALA HB . 0.828 0.021 . . . . . . . . . . . . 15 ALA HB . 0.823 0.048 . . . . . . . . . . . . 18 LEU HD2 . 0.337 0.016 . . . . . . . . . . . . 26 THR HG2 . 0.594 0.023 . . . . . . . . . . . . 27 ILE HD1 . 0.751 0.044 . . . . . . . . . . . . 27 ILE HG2 . 0.751 0.025 . . . . . . . . . . . . 29 THR HG2 . 0.530 0.019 . . . . . . . . . . . . 34 THR HG2 . 0.603 0.017 . . . . . . . . . . . . 35 VAL HG1 . 0.648 0.022 . . . . . . . . . . . . 35 VAL HG2 . 0.600 0.016 . . . . . . . . . . . . 39 LEU HD1 . 0.509 0.036 . . . . . . . . . . . . 39 LEU HD2 . 0.499 0.022 . . . . . . . . . . . . 46 ALA HB . 0.797 0.019 . . . . . . . . . . . . 52 ILE HD1 . 0.306 0.008 . . . . . . . . . . . . 52 ILE HG2 . 0.731 0.021 . . . . . . . . . . . . 55 VAL HG1 . 0.413 0.011 . . . . . . . . . . . . 55 VAL HG2 . 0.389 0.012 . . . . . . . . . . . . 63 ILE HD1 . 0.732 0.024 . . . . . . . . . . . . 63 ILE HG2 . 0.733 0.024 . . . . . . . . . . . . 69 LEU HD1 . 0.269 0.038 . . . . . . . . . . . . 70 THR HG2 . 0.621 0.016 . . . . . . . . . . . . 71 MET HE . 0.342 0.005 . . . . . . . . . . . . 72 MET HE . 0.656 0.014 . . . . . . . . . . . . 73 ALA HB . 0.764 0.023 . . . . . . . . . . . . 76 MET HE . 0.087 0.003 . . . . . . . . . . . . 79 THR HG2 . 0.337 0.009 . . . . . . . . . . . . 85 ILE HD1 . 0.306 0.008 . . . . . . . . . . . . 85 ILE HG2 . 0.632 0.017 . . . . . . . . . . . . 88 ALA HB . 0.787 0.039 . . . . . . . . . . . . 100 ILE HD1 . 0.819 0.042 . . . . . . . . . . . . 102 ALA HB . 0.943 0.026 . . . . . . . . . . . . 103 ALA HB . 0.823 0.022 . . . . . . . . . . . . 105 LEU HD1 . 0.572 0.030 . . . . . . . . . . . . 108 VAL HG1 . 0.497 0.014 . . . . . . . . . . . . 108 VAL HG2 . 0.417 0.010 . . . . . . . . . . . . 109 MET HE . 0.305 0.004 . . . . . . . . . . . . 110 THR HG2 . 0.631 0.015 . . . . . . . . . . . . 112 LEU HD2 . 0.550 0.030 . . . . . . . . . . . . 116 LEU HD1 . 0.170 0.015 . . . . . . . . . . . . 121 VAL HG1 . 0.607 0.014 . . . . . . . . . . . . 124 MET HE . 0.815 0.020 . . . . . . . . . . . . 125 ILE HD1 . 0.376 0.009 . . . . . . . . . . . . 125 ILE HG2 . 0.782 0.022 . . . . . . . . . . . . 128 ALA HB . 0.879 0.045 . . . . . . . . . . . . 130 ILE HD1 . 0.328 0.006 . . . . . . . . . . . . 130 ILE HG2 . 0.545 0.009 . . . . . . . . . . . . 136 VAL HG1 . 0.829 0.039 . . . . . . . . . . . . 142 VAL HG2 . 0.588 0.013 . . . . . . . . . . . . 144 MET HE . 0.330 0.006 . . . . . . . . . . . . 145 MET HE . 0.273 0.005 . . . . . . . . . . . . 146 THR HG2 . 0.491 0.011 . . . . . . . . . . . . 147 ALA HB . 0.341 0.005 . . . . . . . . . . . . stop_ save_ save_S2_parameters_2H_44C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_10 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU HD1 . 0.273 0.016 . . . . . . . . . . . . 9 ILE HD1 . 0.461 0.009 . . . . . . . . . . . . 10 ALA HB . 0.814 0.016 . . . . . . . . . . . . 15 ALA HB . 0.828 0.038 . . . . . . . . . . . . 18 LEU HD2 . 0.349 0.014 . . . . . . . . . . . . 26 THR HG2 . 0.568 0.017 . . . . . . . . . . . . 27 ILE HD1 . 0.732 0.037 . . . . . . . . . . . . 27 ILE HG2 . 0.724 0.021 . . . . . . . . . . . . 29 THR HG2 . 0.509 0.014 . . . . . . . . . . . . 34 THR HG2 . 0.566 0.014 . . . . . . . . . . . . 35 VAL HG1 . 0.648 0.016 . . . . . . . . . . . . 35 VAL HG2 . 0.602 0.013 . . . . . . . . . . . . 39 LEU HD1 . 0.455 0.028 . . . . . . . . . . . . 39 LEU HD2 . 0.490 0.018 . . . . . . . . . . . . 46 ALA HB . 0.803 0.015 . . . . . . . . . . . . 52 ILE HD1 . 0.299 0.007 . . . . . . . . . . . . 52 ILE HG2 . 0.714 0.017 . . . . . . . . . . . . 55 VAL HG1 . 0.375 0.009 . . . . . . . . . . . . 55 VAL HG2 . 0.354 0.010 . . . . . . . . . . . . 63 ILE HD1 . 0.696 0.020 . . . . . . . . . . . . 63 ILE HG2 . 0.740 0.020 . . . . . . . . . . . . 69 LEU HD1 . 0.281 0.031 . . . . . . . . . . . . 70 THR HG2 . 0.572 0.013 . . . . . . . . . . . . 71 MET HE . 0.334 0.005 . . . . . . . . . . . . 72 MET HE . 0.637 0.012 . . . . . . . . . . . . 73 ALA HB . 0.763 0.020 . . . . . . . . . . . . 76 MET HE . 0.076 0.003 . . . . . . . . . . . . 79 THR HG2 . 0.324 0.008 . . . . . . . . . . . . 85 ILE HD1 . 0.291 0.007 . . . . . . . . . . . . 85 ILE HG2 . 0.637 0.014 . . . . . . . . . . . . 88 ALA HB . 0.795 0.031 . . . . . . . . . . . . 100 ILE HD1 . 0.803 0.033 . . . . . . . . . . . . 102 ALA HB . 0.864 0.021 . . . . . . . . . . . . 103 ALA HB . 0.841 0.018 . . . . . . . . . . . . 105 LEU HD1 . 0.520 0.024 . . . . . . . . . . . . 108 VAL HG1 . 0.494 0.012 . . . . . . . . . . . . 108 VAL HG2 . 0.428 0.008 . . . . . . . . . . . . 109 MET HE . 0.288 0.003 . . . . . . . . . . . . 110 THR HG2 . 0.613 0.012 . . . . . . . . . . . . 112 LEU HD2 . 0.526 0.023 . . . . . . . . . . . . 116 LEU HD1 . 0.144 0.012 . . . . . . . . . . . . 121 VAL HG1 . 0.602 0.012 . . . . . . . . . . . . 124 MET HE . 0.777 0.017 . . . . . . . . . . . . 125 ILE HD1 . 0.360 0.008 . . . . . . . . . . . . 125 ILE HG2 . 0.771 0.016 . . . . . . . . . . . . 128 ALA HB . 0.879 0.036 . . . . . . . . . . . . 130 ILE HD1 . 0.317 0.006 . . . . . . . . . . . . 130 ILE HG2 . 0.532 0.007 . . . . . . . . . . . . 136 VAL HG1 . 0.768 0.029 . . . . . . . . . . . . 142 VAL HG2 . 0.554 0.010 . . . . . . . . . . . . 144 MET HE . 0.308 0.006 . . . . . . . . . . . . 145 MET HE . 0.264 0.004 . . . . . . . . . . . . 146 THR HG2 . 0.501 0.009 . . . . . . . . . . . . 147 ALA HB . 0.326 0.005 . . . . . . . . . . . . stop_ save_ save_S2_parameters_2H_47C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_3 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU HD1 . 0.263 0.019 . . . . . . . . . . . . 9 ILE HD1 . 0.466 0.011 . . . . . . . . . . . . 10 ALA HB . 0.841 0.019 . . . . . . . . . . . . 15 ALA HB . 0.805 0.039 . . . . . . . . . . . . 18 LEU HD2 . 0.333 0.014 . . . . . . . . . . . . 26 THR HG2 . 0.558 0.021 . . . . . . . . . . . . 27 ILE HD1 . 0.766 0.038 . . . . . . . . . . . . 27 ILE HG2 . 0.780 0.024 . . . . . . . . . . . . 29 THR HG2 . 0.508 0.017 . . . . . . . . . . . . 34 THR HG2 . 0.584 0.016 . . . . . . . . . . . . 35 VAL HG1 . 0.639 0.019 . . . . . . . . . . . . 35 VAL HG2 . 0.581 0.015 . . . . . . . . . . . . 39 LEU HD1 . 0.455 0.031 . . . . . . . . . . . . 39 LEU HD2 . 0.481 0.020 . . . . . . . . . . . . 46 ALA HB . 0.797 0.020 . . . . . . . . . . . . 52 ILE HD1 . 0.293 0.008 . . . . . . . . . . . . 52 ILE HG2 . 0.720 0.019 . . . . . . . . . . . . 55 VAL HG1 . 0.378 0.011 . . . . . . . . . . . . 55 VAL HG2 . 0.355 0.011 . . . . . . . . . . . . 63 ILE HD1 . 0.705 0.022 . . . . . . . . . . . . 63 ILE HG2 . 0.763 0.023 . . . . . . . . . . . . 69 LEU HD1 . 0.280 0.032 . . . . . . . . . . . . 70 THR HG2 . 0.586 0.014 . . . . . . . . . . . . 71 MET HE . 0.310 0.005 . . . . . . . . . . . . 72 MET HE . 0.619 0.012 . . . . . . . . . . . . 73 ALA HB . 0.790 0.020 . . . . . . . . . . . . 76 MET HE . 0.074 0.003 . . . . . . . . . . . . 79 THR HG2 . 0.313 0.008 . . . . . . . . . . . . 85 ILE HD1 . 0.288 0.009 . . . . . . . . . . . . 85 ILE HG2 . 0.619 0.015 . . . . . . . . . . . . 88 ALA HB . 0.822 0.039 . . . . . . . . . . . . 100 ILE HD1 . 0.821 0.038 . . . . . . . . . . . . 102 ALA HB . 0.923 0.025 . . . . . . . . . . . . 103 ALA HB . 0.808 0.022 . . . . . . . . . . . . 105 LEU HD1 . 0.532 0.027 . . . . . . . . . . . . 108 VAL HG1 . 0.501 0.014 . . . . . . . . . . . . 108 VAL HG2 . 0.447 0.009 . . . . . . . . . . . . 109 MET HE . 0.280 0.004 . . . . . . . . . . . . 110 THR HG2 . 0.611 0.014 . . . . . . . . . . . . 112 LEU HD2 . 0.576 0.025 . . . . . . . . . . . . 116 LEU HD1 . 0.168 0.015 . . . . . . . . . . . . 121 VAL HG1 . 0.601 0.014 . . . . . . . . . . . . 124 MET HE . 0.794 0.015 . . . . . . . . . . . . 125 ILE HD1 . 0.359 0.009 . . . . . . . . . . . . 125 ILE HG2 . 0.784 0.020 . . . . . . . . . . . . 128 ALA HB . 0.912 0.040 . . . . . . . . . . . . 130 ILE HD1 . 0.306 0.007 . . . . . . . . . . . . 130 ILE HG2 . 0.532 0.009 . . . . . . . . . . . . 136 VAL HG1 . 0.797 0.033 . . . . . . . . . . . . 142 VAL HG2 . 0.568 0.013 . . . . . . . . . . . . 144 MET HE . 0.311 0.005 . . . . . . . . . . . . 145 MET HE . 0.265 0.005 . . . . . . . . . . . . 146 THR HG2 . 0.477 0.011 . . . . . . . . . . . . 147 ALA HB . 0.328 0.006 . . . . . . . . . . . . stop_ save_ save_S2_parameters_2H_50C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_11 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU HD1 . 0.264 0.022 . . . . . . . . . . . . 9 ILE HD1 . 0.449 0.013 . . . . . . . . . . . . 10 ALA HB . 0.830 0.022 . . . . . . . . . . . . 15 ALA HB . 0.829 0.049 . . . . . . . . . . . . 18 LEU HD2 . 0.295 0.018 . . . . . . . . . . . . 26 THR HG2 . 0.550 0.021 . . . . . . . . . . . . 27 ILE HD1 . 0.769 0.049 . . . . . . . . . . . . 27 ILE HG2 . 0.689 0.027 . . . . . . . . . . . . 29 THR HG2 . 0.473 0.018 . . . . . . . . . . . . 34 THR HG2 . 0.572 0.018 . . . . . . . . . . . . 35 VAL HG1 . 0.637 0.023 . . . . . . . . . . . . 35 VAL HG2 . 0.585 0.018 . . . . . . . . . . . . 39 LEU HD1 . 0.411 0.036 . . . . . . . . . . . . 39 LEU HD2 . 0.476 0.023 . . . . . . . . . . . . 46 ALA HB . 0.781 0.022 . . . . . . . . . . . . 52 ILE HD1 . 0.285 0.012 . . . . . . . . . . . . 52 ILE HG2 . 0.702 0.022 . . . . . . . . . . . . 55 VAL HG1 . 0.351 0.013 . . . . . . . . . . . . 55 VAL HG2 . 0.344 0.014 . . . . . . . . . . . . 63 ILE HD1 . 0.683 0.025 . . . . . . . . . . . . 63 ILE HG2 . 0.758 0.026 . . . . . . . . . . . . 69 LEU HD1 . 0.224 0.044 . . . . . . . . . . . . 70 THR HG2 . 0.542 0.016 . . . . . . . . . . . . 71 MET HE . 0.294 0.006 . . . . . . . . . . . . 72 MET HE . 0.596 0.016 . . . . . . . . . . . . 73 ALA HB . 0.760 0.025 . . . . . . . . . . . . 76 MET HE . 0.064 0.004 . . . . . . . . . . . . 79 THR HG2 . 0.306 0.011 . . . . . . . . . . . . 85 ILE HD1 . 0.277 0.011 . . . . . . . . . . . . 85 ILE HG2 . 0.595 0.018 . . . . . . . . . . . . 88 ALA HB . 0.800 0.039 . . . . . . . . . . . . 100 ILE HD1 . 0.824 0.046 . . . . . . . . . . . . 102 ALA HB . 0.877 0.028 . . . . . . . . . . . . 103 ALA HB . 0.829 0.023 . . . . . . . . . . . . 105 LEU HD1 . 0.493 0.032 . . . . . . . . . . . . 108 VAL HG1 . 0.503 0.016 . . . . . . . . . . . . 108 VAL HG2 . 0.441 0.009 . . . . . . . . . . . . 109 MET HE . 0.275 0.005 . . . . . . . . . . . . 110 THR HG2 . 0.606 0.015 . . . . . . . . . . . . 112 LEU HD2 . 0.554 0.031 . . . . . . . . . . . . 116 LEU HD1 . 0.177 0.018 . . . . . . . . . . . . 121 VAL HG1 . 0.593 0.015 . . . . . . . . . . . . 124 MET HE . 0.782 0.023 . . . . . . . . . . . . 125 ILE HD1 . 0.336 0.012 . . . . . . . . . . . . 125 ILE HG2 . 0.764 0.022 . . . . . . . . . . . . 128 ALA HB . 0.864 0.044 . . . . . . . . . . . . 130 ILE HD1 . 0.310 0.009 . . . . . . . . . . . . 130 ILE HG2 . 0.509 0.011 . . . . . . . . . . . . 136 VAL HG1 . 0.782 0.037 . . . . . . . . . . . . 142 VAL HG2 . 0.556 0.015 . . . . . . . . . . . . 144 MET HE . 0.286 0.010 . . . . . . . . . . . . 145 MET HE . 0.246 0.005 . . . . . . . . . . . . 146 THR HG2 . 0.474 0.013 . . . . . . . . . . . . 147 ALA HB . 0.320 0.007 . . . . . . . . . . . . stop_ save_ save_S2_parameters_2H_53C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_12 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU HD1 . 0.244 0.017 . . . . . . . . . . . . 9 ILE HD1 . 0.448 0.009 . . . . . . . . . . . . 10 ALA HB . 0.805 0.016 . . . . . . . . . . . . 15 ALA HB . 0.827 0.035 . . . . . . . . . . . . 18 LEU HD2 . 0.307 0.014 . . . . . . . . . . . . 26 THR HG2 . 0.522 0.017 . . . . . . . . . . . . 27 ILE HD1 . 0.760 0.035 . . . . . . . . . . . . 27 ILE HG2 . 0.740 0.020 . . . . . . . . . . . . 29 THR HG2 . 0.475 0.014 . . . . . . . . . . . . 34 THR HG2 . 0.553 0.014 . . . . . . . . . . . . 35 VAL HG1 . 0.617 0.017 . . . . . . . . . . . . 35 VAL HG2 . 0.572 0.013 . . . . . . . . . . . . 39 LEU HD1 . 0.431 0.030 . . . . . . . . . . . . 39 LEU HD2 . 0.459 0.019 . . . . . . . . . . . . 46 ALA HB . 0.759 0.016 . . . . . . . . . . . . 52 ILE HD1 . 0.282 0.007 . . . . . . . . . . . . 52 ILE HG2 . 0.699 0.016 . . . . . . . . . . . . 55 VAL HG1 . 0.362 0.010 . . . . . . . . . . . . 55 VAL HG2 . 0.337 0.011 . . . . . . . . . . . . 63 ILE HD1 . 0.669 0.019 . . . . . . . . . . . . 63 ILE HG2 . 0.751 0.019 . . . . . . . . . . . . 69 LEU HD1 . 0.249 0.033 . . . . . . . . . . . . 70 THR HG2 . 0.533 0.012 . . . . . . . . . . . . 71 MET HE . 0.269 0.005 . . . . . . . . . . . . 72 MET HE . 0.584 0.011 . . . . . . . . . . . . 73 ALA HB . 0.748 0.019 . . . . . . . . . . . . 76 MET HE . 0.071 0.003 . . . . . . . . . . . . 79 THR HG2 . 0.293 0.008 . . . . . . . . . . . . 85 ILE HD1 . 0.281 0.008 . . . . . . . . . . . . 85 ILE HG2 . 0.587 0.013 . . . . . . . . . . . . 88 ALA HB . 0.815 0.030 . . . . . . . . . . . . 100 ILE HD1 . 0.780 0.034 . . . . . . . . . . . . 102 ALA HB . 0.899 0.021 . . . . . . . . . . . . 103 ALA HB . 0.795 0.019 . . . . . . . . . . . . 105 LEU HD1 . 0.495 0.024 . . . . . . . . . . . . 108 VAL HG1 . 0.491 0.012 . . . . . . . . . . . . 109 MET HE . 0.264 0.005 . . . . . . . . . . . . 110 THR HG2 . 0.581 0.013 . . . . . . . . . . . . 112 LEU HD2 . 0.554 0.023 . . . . . . . . . . . . 116 LEU HD1 . 0.148 0.015 . . . . . . . . . . . . 121 VAL HG1 . 0.573 0.012 . . . . . . . . . . . . 124 MET HE . 0.762 0.015 . . . . . . . . . . . . 125 ILE HD1 . 0.339 0.009 . . . . . . . . . . . . 125 ILE HG2 . 0.746 0.016 . . . . . . . . . . . . 128 ALA HB . 0.876 0.032 . . . . . . . . . . . . 130 ILE HD1 . 0.302 0.006 . . . . . . . . . . . . 130 ILE HG2 . 0.508 0.009 . . . . . . . . . . . . 136 VAL HG1 . 0.763 0.028 . . . . . . . . . . . . 142 VAL HG2 . 0.544 0.011 . . . . . . . . . . . . 144 MET HE . 0.283 0.006 . . . . . . . . . . . . 145 MET HE . 0.251 0.005 . . . . . . . . . . . . 146 THR HG2 . 0.462 0.010 . . . . . . . . . . . . 147 ALA HB . 0.314 0.005 . . . . . . . . . . . . stop_ save_ save_S2_parameters_2H_60C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_4 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU HD1 . 0.204 0.023 . . . . . . . . . . . . 9 ILE HD1 . 0.423 0.012 . . . . . . . . . . . . 10 ALA HB . 0.792 0.022 . . . . . . . . . . . . 15 ALA HB . 0.881 0.043 . . . . . . . . . . . . 18 LEU HD2 . 0.277 0.015 . . . . . . . . . . . . 26 THR HG2 . 0.497 0.020 . . . . . . . . . . . . 27 ILE HD1 . 0.754 0.043 . . . . . . . . . . . . 27 ILE HG2 . 0.716 0.024 . . . . . . . . . . . . 29 THR HG2 . 0.443 0.016 . . . . . . . . . . . . 34 THR HG2 . 0.533 0.017 . . . . . . . . . . . . 35 VAL HG1 . 0.628 0.021 . . . . . . . . . . . . 35 VAL HG2 . 0.558 0.017 . . . . . . . . . . . . 39 LEU HD1 . 0.426 0.033 . . . . . . . . . . . . 39 LEU HD2 . 0.468 0.022 . . . . . . . . . . . . 46 ALA HB . 0.769 0.022 . . . . . . . . . . . . 52 ILE HD1 . 0.245 0.009 . . . . . . . . . . . . 52 ILE HG2 . 0.665 0.019 . . . . . . . . . . . . 55 VAL HG1 . 0.313 0.012 . . . . . . . . . . . . 55 VAL HG2 . 0.303 0.012 . . . . . . . . . . . . 63 ILE HD1 . 0.645 0.023 . . . . . . . . . . . . 63 ILE HG2 . 0.717 0.025 . . . . . . . . . . . . 69 LEU HD1 . 0.242 0.038 . . . . . . . . . . . . 70 THR HG2 . 0.491 0.015 . . . . . . . . . . . . 71 MET HE . 0.214 0.005 . . . . . . . . . . . . 72 MET HE . 0.523 0.013 . . . . . . . . . . . . 73 ALA HB . 0.733 0.023 . . . . . . . . . . . . 76 MET HE . 0.068 0.003 . . . . . . . . . . . . 79 THR HG2 . 0.249 0.009 . . . . . . . . . . . . 85 ILE HD1 . 0.276 0.011 . . . . . . . . . . . . 85 ILE HG2 . 0.587 0.016 . . . . . . . . . . . . 88 ALA HB . 0.805 0.040 . . . . . . . . . . . . 100 ILE HD1 . 0.796 0.041 . . . . . . . . . . . . 102 ALA HB . 0.905 0.029 . . . . . . . . . . . . 103 ALA HB . 0.799 0.024 . . . . . . . . . . . . 105 LEU HD1 . 0.445 0.026 . . . . . . . . . . . . 108 VAL HG1 . 0.509 0.015 . . . . . . . . . . . . 109 MET HE . 0.237 0.005 . . . . . . . . . . . . 110 THR HG2 . 0.575 0.016 . . . . . . . . . . . . 112 LEU HD2 . 0.495 0.027 . . . . . . . . . . . . 116 LEU HD1 . 0.143 0.017 . . . . . . . . . . . . 121 VAL HG1 . 0.552 0.014 . . . . . . . . . . . . 124 MET HE . 0.741 0.018 . . . . . . . . . . . . 125 ILE HD1 . 0.314 0.011 . . . . . . . . . . . . 125 ILE HG2 . 0.741 0.021 . . . . . . . . . . . . 128 ALA HB . 0.854 0.041 . . . . . . . . . . . . 130 ILE HD1 . 0.291 0.009 . . . . . . . . . . . . 130 ILE HG2 . 0.473 0.011 . . . . . . . . . . . . 136 VAL HG1 . 0.779 0.034 . . . . . . . . . . . . 142 VAL HG2 . 0.505 0.014 . . . . . . . . . . . . 144 MET HE . 0.258 0.007 . . . . . . . . . . . . 145 MET HE . 0.241 0.009 . . . . . . . . . . . . 146 THR HG2 . 0.440 0.012 . . . . . . . . . . . . 147 ALA HB . 0.291 0.007 . . . . . . . . . . . . stop_ save_ save_S2_parameters_2H_67C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_13 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU HD1 . 0.186 0.027 . . . . . . . . . . . . 9 ILE HD1 . 0.399 0.014 . . . . . . . . . . . . 10 ALA HB . 0.795 0.023 . . . . . . . . . . . . 15 ALA HB . 0.863 0.051 . . . . . . . . . . . . 18 LEU HD2 . 0.252 0.017 . . . . . . . . . . . . 26 THR HG2 . 0.499 0.019 . . . . . . . . . . . . 27 ILE HD1 . 0.747 0.049 . . . . . . . . . . . . 27 ILE HG2 . 0.649 0.023 . . . . . . . . . . . . 29 THR HG2 . 0.439 0.017 . . . . . . . . . . . . 34 THR HG2 . 0.526 0.018 . . . . . . . . . . . . 35 VAL HG1 . 0.580 0.023 . . . . . . . . . . . . 35 VAL HG2 . 0.562 0.019 . . . . . . . . . . . . 39 LEU HD1 . 0.363 0.036 . . . . . . . . . . . . 39 LEU HD2 . 0.441 0.023 . . . . . . . . . . . . 46 ALA HB . 0.750 0.022 . . . . . . . . . . . . 52 ILE HD1 . 0.250 0.011 . . . . . . . . . . . . 52 ILE HG2 . 0.659 0.021 . . . . . . . . . . . . 55 VAL HG1 . 0.305 0.013 . . . . . . . . . . . . 55 VAL HG2 . 0.271 0.014 . . . . . . . . . . . . 63 ILE HD1 . 0.601 0.023 . . . . . . . . . . . . 63 ILE HG2 . 0.726 0.025 . . . . . . . . . . . . 69 LEU HD1 . 0.233 0.047 . . . . . . . . . . . . 70 THR HG2 . 0.436 0.014 . . . . . . . . . . . . 71 MET HE . 0.206 0.005 . . . . . . . . . . . . 72 MET HE . 0.514 0.018 . . . . . . . . . . . . 73 ALA HB . 0.761 0.032 . . . . . . . . . . . . 76 MET HE . 0.063 0.005 . . . . . . . . . . . . 79 THR HG2 . 0.229 0.013 . . . . . . . . . . . . 85 ILE HD1 . 0.263 0.014 . . . . . . . . . . . . 85 ILE HG2 . 0.570 0.018 . . . . . . . . . . . . 88 ALA HB . 0.814 0.039 . . . . . . . . . . . . 100 ILE HD1 . 0.755 0.046 . . . . . . . . . . . . 102 ALA HB . 0.867 0.028 . . . . . . . . . . . . 103 ALA HB . 0.786 0.023 . . . . . . . . . . . . 105 LEU HD1 . 0.427 0.030 . . . . . . . . . . . . 108 VAL HG1 . 0.517 0.017 . . . . . . . . . . . . 109 MET HE . 0.231 0.006 . . . . . . . . . . . . 110 THR HG2 . 0.561 0.016 . . . . . . . . . . . . 112 LEU HD2 . 0.462 0.029 . . . . . . . . . . . . 116 LEU HD1 . 0.159 0.021 . . . . . . . . . . . . 121 VAL HG1 . 0.517 0.015 . . . . . . . . . . . . 124 MET HE . 0.710 0.028 . . . . . . . . . . . . 125 ILE HD1 . 0.283 0.013 . . . . . . . . . . . . 125 ILE HG2 . 0.717 0.022 . . . . . . . . . . . . 128 ALA HB . 0.849 0.043 . . . . . . . . . . . . 130 ILE HD1 . 0.286 0.010 . . . . . . . . . . . . 130 ILE HG2 . 0.453 0.012 . . . . . . . . . . . . 136 VAL HG1 . 0.735 0.037 . . . . . . . . . . . . 142 VAL HG2 . 0.512 0.015 . . . . . . . . . . . . 144 MET HE . 0.225 0.012 . . . . . . . . . . . . 145 MET HE . 0.231 0.007 . . . . . . . . . . . . 146 THR HG2 . 0.430 0.014 . . . . . . . . . . . . 147 ALA HB . 0.289 0.008 . . . . . . . . . . . . stop_ save_ save_S2_parameters_2H_73C _Saveframe_category S2_parameters _Details . loop_ _Sample_label $sample_one stop_ _Sample_conditions_label $sample_conditions_5 _Mol_system_component_name Calmodulin _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 4 LEU HD1 . 0.159 0.046 . . . . . . . . . . . . 10 ALA HB . 0.787 0.032 . . . . . . . . . . . . 15 ALA HB . 0.756 0.086 . . . . . . . . . . . . 18 LEU HD2 . 0.266 0.032 . . . . . . . . . . . . 26 THR HG2 . 0.465 0.024 . . . . . . . . . . . . 27 ILE HD1 . 0.683 0.067 . . . . . . . . . . . . 27 ILE HG2 . 0.564 0.029 . . . . . . . . . . . . 29 THR HG2 . 0.422 0.021 . . . . . . . . . . . . 34 THR HG2 . 0.501 0.023 . . . . . . . . . . . . 35 VAL HG1 . 0.577 0.033 . . . . . . . . . . . . 35 VAL HG2 . 0.532 0.030 . . . . . . . . . . . . 39 LEU HD1 . 0.367 0.050 . . . . . . . . . . . . 39 LEU HD2 . 0.381 0.034 . . . . . . . . . . . . 46 ALA HB . 0.729 0.030 . . . . . . . . . . . . 52 ILE HD1 . 0.245 0.015 . . . . . . . . . . . . 52 ILE HG2 . 0.614 0.028 . . . . . . . . . . . . 55 VAL HG1 . 0.293 0.018 . . . . . . . . . . . . 55 VAL HG2 . 0.224 0.019 . . . . . . . . . . . . 63 ILE HD1 . 0.556 0.031 . . . . . . . . . . . . 63 ILE HG2 . 0.717 0.036 . . . . . . . . . . . . 69 LEU HD1 . 0.250 0.075 . . . . . . . . . . . . 70 THR HG2 . 0.262 0.012 . . . . . . . . . . . . 71 MET HE . 0.158 0.011 . . . . . . . . . . . . 72 MET HE . 0.454 0.035 . . . . . . . . . . . . 73 ALA HB . 0.715 0.056 . . . . . . . . . . . . 79 THR HG2 . 0.193 0.021 . . . . . . . . . . . . 85 ILE HD1 . 0.258 0.023 . . . . . . . . . . . . 85 ILE HG2 . 0.588 0.026 . . . . . . . . . . . . 88 ALA HB . 0.838 0.053 . . . . . . . . . . . . 100 ILE HD1 . 0.752 0.068 . . . . . . . . . . . . 102 ALA HB . 0.850 0.039 . . . . . . . . . . . . 103 ALA HB . 0.746 0.031 . . . . . . . . . . . . 105 LEU HD1 . 0.371 0.045 . . . . . . . . . . . . 108 VAL HG1 . 0.515 0.026 . . . . . . . . . . . . 109 MET HE . 0.220 0.010 . . . . . . . . . . . . 110 THR HG2 . 0.542 0.023 . . . . . . . . . . . . 112 LEU HD2 . 0.466 0.041 . . . . . . . . . . . . 116 LEU HD1 . 0.108 0.032 . . . . . . . . . . . . 121 VAL HG1 . 0.518 0.022 . . . . . . . . . . . . 124 MET HE . 0.711 0.050 . . . . . . . . . . . . 125 ILE HD1 . 0.261 0.020 . . . . . . . . . . . . 125 ILE HG2 . 0.683 0.028 . . . . . . . . . . . . 128 ALA HB . 0.906 0.073 . . . . . . . . . . . . 130 ILE HG2 . 0.446 0.016 . . . . . . . . . . . . 136 VAL HG1 . 0.727 0.052 . . . . . . . . . . . . 142 VAL HG2 . 0.504 0.021 . . . . . . . . . . . . 144 MET HE . 0.214 0.023 . . . . . . . . . . . . 145 MET HE . 0.235 0.014 . . . . . . . . . . . . 146 THR HG2 . 0.441 0.018 . . . . . . . . . . . . 147 ALA HB . 0.286 0.011 . . . . . . . . . . . . stop_ save_