data_34100 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; NMR solution structure of the TSL2 RNA hairpin ; _BMRB_accession_number 34100 _BMRB_flat_file_name bmr34100.str _Entry_type original _Submission_date 2017-02-13 _Accession_date 2017-02-13 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Garcia-Lopez A. . . 2 Wacker A. . . 3 Tessaro F. . . 4 Jonker H. R.A. . 5 Richter C. . . 6 Comte A. . . 7 Berntenis N. . . 8 Schmucki R. . . 9 Hatje K. . . 10 Sciarra D. . . 11 Konieczny P. . . 12 Fournet G. . . 13 Faustino I. . . 14 Orozco M. . . 15 Artero R. . . 16 Goekjian P. . . 17 Metzger F. . . 18 Ebeling M. . . 19 Joseph B. . . 20 Schwalbe H. . . 21 Scapozza L. . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 150 "13C chemical shifts" 67 "15N chemical shifts" 4 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2018-03-06 original BMRB . stop_ _Original_release_date 2017-02-16 save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Rational targeting of RNA structure in SMN2 transcripts reverses Spinal Muscular Atrophy molecular phenotypes ; _Citation_status 'in preparation' _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Garcia-Lopez A. . . 2 Wacker A. . . 3 Tessaro F. . . 4 Jonker H. R.A. . 5 Richter C. . . 6 Comte A. . . 7 Berntenis N. . . 8 Schmucki R. . . 9 Hatje K. . . 10 Sciarra D. . . 11 Konieczny P. . . 12 Fournet G. . . 13 Faustino I. . . 14 Orozco M. . . 15 Artero R. . . 16 Goekjian P. . . 17 Metzger F. . . 18 Ebeling M. . . 19 Joseph B. . . 20 Schwalbe H. . . 21 Scapozza L. . . stop_ _Journal_abbreviation 'Nat. Commun.' _Journal_volume . _Journal_issue . _Journal_CSD 0353 _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'RNA (19-MER)' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label entity_1 $entity_1 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common entity_1 _Molecular_mass 6048.626 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 19 _Mol_residue_sequence ; AUUCCUUAAAUUAAGGAGU ; loop_ _Residue_seq_code _Residue_label 1 A 2 U 3 U 4 C 5 C 6 U 7 U 8 A 9 A 10 A 11 U 12 U 13 A 14 A 15 G 16 G 17 A 18 G 19 U stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $entity_1 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $entity_1 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details '0.6 mM RNA (19-MER), 50 mM sodium phosphate, 0.1 mM EDTA, 90% H2O/10% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling EDTA 0.1 mM 'natural abundance' $entity_1 0.6 mM 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details '0.6 mM RNA (19-MER), 50 mM sodium phosphate, 0.1 mM EDTA, 99.8% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling EDTA 0.1 mM 'natural abundance' $entity_1 0.6 mM 'natural abundance' 'sodium phosphate' 50 mM 'natural abundance' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details '0.6 mM [U-98% 13C; U-96-98% 15N]-ADE RNA (19-MER), 50 mM sodium phosphate, 0.1 mM EDTA, 99.8% D2O' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling EDTA 0.1 mM 'natural abundance' $entity_1 0.6 mM '[U-98% 13C; U-96-98% 15N]-ADE' 'sodium phosphate' 50 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name ARIA _Version '1.2 HJ development version' loop_ _Vendor _Address _Electronic_address "Linge, O'Donoghue and Nilges" . . stop_ loop_ _Task refinement stop_ _Details . save_ save_software_2 _Saveframe_category software _Name CNS _Version 1.1 loop_ _Vendor _Address _Electronic_address 'Brunger, Adams, Clore, Gros, Nilges and Read' . . stop_ loop_ _Task 'structure calculation' stop_ _Details . save_ save_software_3 _Saveframe_category software _Name SPARKY _Version 3.114 loop_ _Vendor _Address _Electronic_address 'Kneller and Goddard' . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' 'peak picking' stop_ _Details . save_ save_software_4 _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task processing stop_ _Details . save_ save_software_5 _Saveframe_category software _Name TOPSPIN _Version 3.2 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details cryoprobe save_ save_NMR_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details cryoprobe save_ save_NMR_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 900 _Details cryoprobe save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_2D_1H-1H_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_3D_1H-13C_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_3 save_ save_2D_12C-filtered_1H-1H_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 12C-filtered 1H-1H NOESY' _Sample_label $sample_3 save_ save_2D_13C-editted_1H-1H_NOESY_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 13C-editted 1H-1H NOESY' _Sample_label $sample_3 save_ save_2D_1H-1H_TOCSY_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_2 save_ save_2D_1H-1H_COSY_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H COSY' _Sample_label $sample_2 save_ save_2D_1H-13C_HSQC_9 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_2 save_ save_2D_1H-13C_HSQC_aromatic_10 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC aromatic' _Sample_label $sample_3 save_ save_2D_1H-13C_HSQC_sugar_11 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC sugar' _Sample_label $sample_3 save_ save_2D_1H-15N_HSQC_12 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HCCH-TOCSY_13 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCCH-TOCSY' _Sample_label $sample_3 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 74 . mM pH 6.4 . pH pressure 1 . atm temperature 298 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 71 . mM pH 6.4 . pH pressure 1 . atm temperature 278 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water C 13 protons ppm 4.7 internal indirect . . . 0.25144953 water H 1 protons ppm 4.7 internal direct . . . 1.0 water N 15 protons ppm 4.7 internal indirect . . . 0.10132912 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details 'Imino and Amino at 278K' loop_ _Experiment_label '2D 1H-1H NOESY' '3D 1H-13C NOESY' '2D 12C-filtered 1H-1H NOESY' '2D 13C-editted 1H-1H NOESY' '2D 1H-1H TOCSY' '2D 1H-1H COSY' '2D 1H-13C HSQC' '2D 1H-13C HSQC aromatic' '2D 1H-13C HSQC sugar' '2D 1H-15N HSQC' '3D HCCH-TOCSY' stop_ loop_ _Sample_label $sample_2 $sample_1 $sample_3 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name entity_1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 A H1' H 5.96 0.02 1 2 1 1 A H2 H 8.10 0.02 1 3 1 1 A H2' H 4.83 0.02 1 4 1 1 A H3' H 4.62 0.02 1 5 1 1 A H4' H 4.38 0.02 1 6 1 1 A H5' H 3.95 0.02 2 7 1 1 A H5'' H 3.84 0.02 2 8 1 1 A H8 H 8.25 0.02 1 9 1 1 A C1' C 92.0 0.10 1 10 1 1 A C2 C 154.9 0.10 1 11 1 1 A C2' C 75.7 0.10 1 12 1 1 A C3' C 75.9 0.10 1 13 1 1 A C4' C 86.5 0.10 1 14 1 1 A C5' C 63.2 0.10 1 15 1 1 A C8 C 142.6 0.10 1 16 2 2 U H1' H 5.79 0.02 1 17 2 2 U H2' H 4.34 0.02 1 18 2 2 U H3' H 4.63 0.02 1 19 2 2 U H4' H 4.49 0.02 1 20 2 2 U H5 H 5.66 0.02 1 21 2 2 U H5' H 4.13 0.02 2 22 2 2 U H5'' H 4.13 0.02 2 23 2 2 U H6 H 7.81 0.02 1 24 3 3 U H1' H 5.51 0.02 1 25 3 3 U H2' H 4.54 0.02 1 26 3 3 U H3' H 4.43 0.02 1 27 3 3 U H5 H 5.80 0.02 1 28 3 3 U H5' H 4.15 0.02 2 29 3 3 U H5'' H 4.15 0.02 2 30 3 3 U H6 H 8.04 0.02 1 31 3 3 U C1' C 93.5 0.10 1 32 3 3 U C5 C 104.0 0.10 1 33 4 4 C H1' H 5.56 0.02 1 34 4 4 C H2' H 4.36 0.02 1 35 4 4 C H3' H 4.44 0.02 1 36 4 4 C H5 H 5.72 0.02 1 37 4 4 C H5' H 4.13 0.02 2 38 4 4 C H5'' H 4.13 0.02 2 39 4 4 C H6 H 7.89 0.02 1 40 4 4 C H41 H 8.16 0.02 2 41 4 4 C H42 H 6.76 0.02 2 42 4 4 C C1' C 94.2 0.10 1 43 5 5 C H1' H 5.40 0.02 1 44 5 5 C H2' H 4.30 0.02 1 45 5 5 C H3' H 4.43 0.02 1 46 5 5 C H5 H 5.47 0.02 1 47 5 5 C H5' H 4.04 0.02 2 48 5 5 C H5'' H 4.04 0.02 2 49 5 5 C H6 H 7.76 0.02 1 50 5 5 C H41 H 8.12 0.02 2 51 5 5 C H42 H 6.64 0.02 2 52 5 5 C C1' C 94.3 0.10 1 53 6 6 U H1' H 5.44 0.02 1 54 6 6 U H2' H 4.32 0.02 1 55 6 6 U H3 H 13.64 0.02 1 56 6 6 U H5 H 5.32 0.02 1 57 6 6 U H5' H 4.03 0.02 2 58 6 6 U H5'' H 4.03 0.02 2 59 6 6 U H6 H 7.82 0.02 1 60 6 6 U C1' C 94.0 0.10 1 61 6 6 U N3 N 161.9 0.10 1 62 7 7 U H1' H 5.55 0.02 1 63 7 7 U H2' H 4.37 0.02 1 64 7 7 U H3 H 12.59 0.02 1 65 7 7 U H3' H 4.53 0.02 1 66 7 7 U H5 H 5.50 0.02 1 67 7 7 U H6 H 7.84 0.02 1 68 7 7 U C1' C 93.2 0.10 1 69 7 7 U C5 C 103.6 0.10 1 70 7 7 U C6 C 143.5 0.10 1 71 7 7 U N3 N 161.0 0.10 1 72 8 8 A H1' H 5.71 0.02 1 73 8 8 A H2 H 6.85 0.02 1 74 8 8 A H2' H 4.52 0.02 1 75 8 8 A H3' H 4.49 0.02 1 76 8 8 A H4' H 4.44 0.02 1 77 8 8 A H5' H 4.37 0.02 2 78 8 8 A H5'' H 4.11 0.02 2 79 8 8 A H8 H 7.95 0.02 1 80 8 8 A C1' C 92.4 0.10 1 81 8 8 A C2 C 153.1 0.10 1 82 8 8 A C2' C 75.7 0.10 1 83 8 8 A C3' C 74.1 0.10 1 84 8 8 A C4' C 82.8 0.10 1 85 8 8 A C5' C 66.4 0.10 1 86 8 8 A C8 C 139.8 0.10 1 87 9 9 A H1' H 5.25 0.02 1 88 9 9 A H2 H 7.94 0.02 1 89 9 9 A H2' H 4.41 0.02 1 90 9 9 A H3' H 4.36 0.02 1 91 9 9 A H4' H 4.25 0.02 1 92 9 9 A H5' H 4.19 0.02 2 93 9 9 A H5'' H 3.95 0.02 2 94 9 9 A H8 H 7.63 0.02 1 95 9 9 A C1' C 91.5 0.10 1 96 9 9 A C2 C 155.1 0.10 1 97 9 9 A C2' C 75.5 0.10 1 98 9 9 A C3' C 75.4 0.10 1 99 9 9 A C4' C 83.9 0.10 1 100 9 9 A C5' C 66.4 0.10 1 101 9 9 A C8 C 140.1 0.10 1 102 10 10 A H1' H 5.66 0.02 1 103 10 10 A H2 H 7.60 0.02 1 104 10 10 A H2' H 4.42 0.02 1 105 10 10 A H3' H 4.57 0.02 1 106 10 10 A H4' H 4.40 0.02 1 107 10 10 A H5' H 4.13 0.02 2 108 10 10 A H5'' H 3.92 0.02 2 109 10 10 A H8 H 8.00 0.02 1 110 10 10 A H61 H 5.67 0.02 2 111 10 10 A H62 H 5.67 0.02 2 112 10 10 A C1' C 89.4 0.10 1 113 10 10 A C2 C 154.6 0.10 1 114 10 10 A C2' C 77.5 0.10 1 115 10 10 A C3' C 78.7 0.10 1 116 10 10 A C4' C 85.8 0.10 1 117 10 10 A C5' C 67.5 0.10 1 118 10 10 A C8 C 141.2 0.10 1 119 11 11 U H1' H 5.96 0.02 1 120 11 11 U H2' H 4.35 0.02 1 121 11 11 U H3' H 4.68 0.02 1 122 11 11 U H4' H 4.48 0.02 1 123 11 11 U H5 H 5.82 0.02 1 124 11 11 U H5' H 4.11 0.02 2 125 11 11 U H5'' H 4.09 0.02 2 126 11 11 U H6 H 7.74 0.02 1 127 11 11 U C5 C 105.9 0.10 1 128 12 12 U H1' H 5.82 0.02 1 129 12 12 U H2' H 4.55 0.02 1 130 12 12 U H3' H 4.54 0.02 1 131 12 12 U H4' H 4.50 0.02 1 132 12 12 U H5 H 5.89 0.02 1 133 12 12 U H5' H 4.23 0.02 2 134 12 12 U H5'' H 4.20 0.02 2 135 12 12 U H6 H 7.84 0.02 1 136 12 12 U C6 C 142.1 0.10 1 137 13 13 A H1' H 5.88 0.02 1 138 13 13 A H2 H 6.59 0.02 1 139 13 13 A H2' H 4.59 0.02 1 140 13 13 A H3' H 4.58 0.02 1 141 13 13 A H4' H 4.54 0.02 1 142 13 13 A H5' H 4.39 0.02 2 143 13 13 A H5'' H 4.30 0.02 2 144 13 13 A H8 H 8.18 0.02 1 145 13 13 A H61 H 7.11 0.02 2 146 13 13 A H62 H 7.11 0.02 2 147 13 13 A C1' C 92.5 0.10 1 148 13 13 A C2 C 152.1 0.10 1 149 13 13 A C2' C 74.2 0.10 1 150 13 13 A C3' C 75.4 0.10 1 151 13 13 A C4' C 83.0 0.10 1 152 13 13 A C5' C 67.5 0.10 1 153 13 13 A C8 C 140.8 0.10 1 154 14 14 A H1' H 5.83 0.02 1 155 14 14 A H2 H 7.32 0.02 1 156 14 14 A H2' H 4.52 0.02 1 157 14 14 A H3' H 4.57 0.02 1 158 14 14 A H4' H 4.44 0.02 1 159 14 14 A H5' H 4.50 0.02 2 160 14 14 A H5'' H 4.13 0.02 2 161 14 14 A H8 H 7.73 0.02 1 162 14 14 A H61 H 7.50 0.02 2 163 14 14 A H62 H 6.16 0.02 2 164 14 14 A C1' C 92.3 0.10 1 165 14 14 A C2 C 153.1 0.10 1 166 14 14 A C2' C 75.7 0.10 1 167 14 14 A C3' C 72.8 0.10 1 168 14 14 A C4' C 82.0 0.10 1 169 14 14 A C5' C 65.4 0.10 1 170 14 14 A C8 C 139.2 0.10 1 171 15 15 G H1 H 12.51 0.02 1 172 15 15 G H1' H 5.48 0.02 1 173 15 15 G H2' H 4.40 0.02 1 174 15 15 G H3' H 3.98 0.02 1 175 15 15 G H8 H 6.95 0.02 1 176 15 15 G H21 H 7.96 0.02 2 177 15 15 G H22 H 5.77 0.02 2 178 15 15 G C1' C 92.3 0.10 1 179 15 15 G N1 N 147.1 0.10 1 180 16 16 G H1 H 12.05 0.02 1 181 16 16 G H1' H 5.62 0.02 1 182 16 16 G H2' H 4.50 0.02 1 183 16 16 G H3' H 4.43 0.02 1 184 16 16 G H8 H 7.03 0.02 1 185 16 16 G H21 H 7.70 0.02 2 186 16 16 G H22 H 7.70 0.02 2 187 16 16 G C1' C 92.8 0.10 1 188 16 16 G N1 N 146.9 0.10 1 189 17 17 A H1' H 5.89 0.02 1 190 17 17 A H2 H 7.60 0.02 1 191 17 17 A H2' H 4.56 0.02 1 192 17 17 A H3' H 4.45 0.02 1 193 17 17 A H4' H 4.40 0.02 1 194 17 17 A H5' H 4.43 0.02 2 195 17 17 A H5'' H 4.02 0.02 2 196 17 17 A H8 H 7.50 0.02 1 197 17 17 A C1' C 92.9 0.10 1 198 17 17 A C2 C 153.6 0.10 1 199 17 17 A C2' C 75.8 0.10 1 200 17 17 A C3' C 73.0 0.10 1 201 17 17 A C4' C 82.1 0.10 1 202 17 17 A C5' C 65.3 0.10 1 203 17 17 A C8 C 139.0 0.10 1 204 18 18 G H1' H 5.46 0.02 1 205 18 18 G H2' H 4.31 0.02 1 206 18 18 G H3' H 3.96 0.02 1 207 18 18 G H8 H 7.17 0.02 1 208 18 18 G C1' C 92.0 0.10 1 209 18 18 G C8 C 136.9 0.10 1 210 19 19 U H1' H 5.62 0.02 1 211 19 19 U H2' H 3.98 0.02 1 212 19 19 U H3' H 4.09 0.02 1 213 19 19 U H4' H 4.06 0.02 1 214 19 19 U H5 H 5.26 0.02 1 215 19 19 U H5' H 4.21 0.02 2 216 19 19 U H5'' H 4.21 0.02 2 217 19 19 U H6 H 7.52 0.02 1 218 19 19 U C1' C 91.7 0.10 1 219 19 19 U C4' C 84.8 0.10 1 220 19 19 U C5 C 104.1 0.10 1 221 19 19 U C6 C 142.5 0.10 1 stop_ save_