data_30637 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structural Basis for Client Recognition and Activity of Hsp40 Chaperones ; _BMRB_accession_number 30637 _BMRB_flat_file_name bmr30637.str _Entry_type original _Submission_date 2019-07-10 _Accession_date 2019-07-10 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jiang Y. . . 2 Rossi P. . . 3 Kalodimos C. G. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 230 "13C chemical shifts" 311 "15N chemical shifts" 132 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2019-09-11 original BMRB . stop_ _Original_release_date 2019-08-07 save_ ############################# # Citation for this entry # ############################# save_citation_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structural Basis for Client Recognition and Activity of Hsp40 Chaperones ; _Citation_status 'in preparation' _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jiang Y. . . 2 Rossi P. . . 3 Kalodimos C. G. . stop_ _Journal_abbreviation . _Journal_volume . _Journal_issue . _Journal_CSD 0353 _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Alkaline phosphatase,Chaperone protein DnaJ 2 (E.C.3.1.3.1)' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label entity_1 $entity_1 stop_ _System_molecular_weight . _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_entity_1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common entity_1 _Molecular_mass 16625.180 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 161 _Mol_residue_sequence ; AALVAHVTSGSGGSGGSGGS GRDLRAELPLTLEEAFHGGE RVVEVAGRRVSVRIPPGVRE GSVIRVPGMGGQGNPPGDLL LVVRLLPHPVFRLEGQDLYA TLDVPAPIAVVGGKVRAMTL EGPVEVAVPPRTQAGRKLRL KGKGFPGPAGRGDLYLEVRI T ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 96 ALA 2 97 ALA 3 98 LEU 4 99 VAL 5 100 ALA 6 101 HIS 7 102 VAL 8 103 THR 9 104 SER 10 105 GLY 11 106 SER 12 107 GLY 13 108 GLY 14 109 SER 15 110 GLY 16 111 GLY 17 112 SER 18 113 GLY 19 114 GLY 20 115 SER 21 116 GLY 22 117 ARG 23 118 ASP 24 119 LEU 25 120 ARG 26 121 ALA 27 122 GLU 28 123 LEU 29 124 PRO 30 125 LEU 31 126 THR 32 127 LEU 33 128 GLU 34 129 GLU 35 130 ALA 36 131 PHE 37 132 HIS 38 133 GLY 39 134 GLY 40 135 GLU 41 136 ARG 42 137 VAL 43 138 VAL 44 139 GLU 45 140 VAL 46 141 ALA 47 142 GLY 48 143 ARG 49 144 ARG 50 145 VAL 51 146 SER 52 147 VAL 53 148 ARG 54 149 ILE 55 150 PRO 56 151 PRO 57 152 GLY 58 153 VAL 59 154 ARG 60 155 GLU 61 156 GLY 62 157 SER 63 158 VAL 64 159 ILE 65 160 ARG 66 161 VAL 67 162 PRO 68 163 GLY 69 164 MET 70 165 GLY 71 166 GLY 72 167 GLN 73 168 GLY 74 169 ASN 75 170 PRO 76 171 PRO 77 172 GLY 78 173 ASP 79 174 LEU 80 175 LEU 81 176 LEU 82 177 VAL 83 178 VAL 84 179 ARG 85 180 LEU 86 181 LEU 87 182 PRO 88 183 HIS 89 184 PRO 90 185 VAL 91 186 PHE 92 187 ARG 93 188 LEU 94 189 GLU 95 190 GLY 96 191 GLN 97 192 ASP 98 193 LEU 99 194 TYR 100 195 ALA 101 196 THR 102 197 LEU 103 198 ASP 104 199 VAL 105 200 PRO 106 201 ALA 107 202 PRO 108 203 ILE 109 204 ALA 110 205 VAL 111 206 VAL 112 207 GLY 113 208 GLY 114 209 LYS 115 210 VAL 116 211 ARG 117 212 ALA 118 213 MET 119 214 THR 120 215 LEU 121 216 GLU 122 217 GLY 123 218 PRO 124 219 VAL 125 220 GLU 126 221 VAL 127 222 ALA 128 223 VAL 129 224 PRO 130 225 PRO 131 226 ARG 132 227 THR 133 228 GLN 134 229 ALA 135 230 GLY 136 231 ARG 137 232 LYS 138 233 LEU 139 234 ARG 140 235 LEU 141 236 LYS 142 237 GLY 143 238 LYS 144 239 GLY 145 240 PHE 146 241 PRO 147 242 GLY 148 243 PRO 149 244 ALA 150 245 GLY 151 246 ARG 152 247 GLY 153 248 ASP 154 249 LEU 155 250 TYR 156 251 LEU 157 252 GLU 158 253 VAL 159 254 ARG 160 255 ILE 161 256 THR stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Strain _Gene_mnemonic $entity_1 'E. coli' 83333 Bacteria . Escherichia coli K12 'phoA, b0383, JW0374' $entity_1 'Thermus thermophilus' 300852 Bacteria . Thermus thermophilus 'HB8 / ATCC 27634 / DSM 579' 'dnaJ2, TTHA1489' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $entity_1 'recombinant technology' . . . BL21(DE3) . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details ; 1 mM [ILVMAT-sel-1H-13C_methyls; U-15N; U-2H] C-CBD1-CBD2, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O ; loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 1 mM '[ILVMAT-sel-1H-13C_methyls; U-15N; U-2H]' 'potassium chloride' 75 mM 'natural abundance' 'potassium phosphate' 20 mM 'natural abundance' 'sodium azide' 0.04 % 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details ; 1 mM [U-100% 13C; U-100% 15N] C-CBD1-CBD2, 75 mM potassium chloride, 20 mM potassium phosphate, 0.04 % sodium azide, 90% H2O/10% D2O ; loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $entity_1 1 mM '[U-100% 13C; U-100% 15N]' 'potassium chloride' 75 mM 'natural abundance' 'potassium phosphate' 20 mM 'natural abundance' 'sodium azide' 0.04 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software_1 _Saveframe_category software _Name CNS _Version . loop_ _Vendor _Address _Electronic_address 'Brunger, Adams, Clore, Gros, Nilges and Read' . . stop_ loop_ _Task refinement stop_ _Details . save_ save_software_2 _Saveframe_category software _Name CYANA _Version . loop_ _Vendor _Address _Electronic_address 'Guntert, Mumenthaler and Wuthrich' . . stop_ loop_ _Task 'structure calculation' stop_ _Details . save_ save_software_3 _Saveframe_category software _Name PINE _Version . loop_ _Vendor _Address _Electronic_address 'Bahrami, Markley, Assadi, and Eghbalnia' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_software_4 _Saveframe_category software _Name Sparky _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'peak picking' stop_ _Details . save_ save_software_5 _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_software_6 _Saveframe_category software _Name TALOS _Version . loop_ _Vendor _Address _Electronic_address 'Cornilescu, Delaglio and Bax' . . stop_ loop_ _Task 'geometry optimization' stop_ _Details . save_ save_software_7 _Saveframe_category software _Name TopSpin _Version 4.0 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ save_software_8 _Saveframe_category software _Name PSVS _Version . loop_ _Vendor _Address _Electronic_address 'Bhattacharya and Montelione' . . stop_ loop_ _Task 'geometry optimization' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_NMR_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model 'AVANCE NEO' _Field_strength 700 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_2 save_ save_2D_1H-13C_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_2 save_ save_2D_1H-13C_HMQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HMQC' _Sample_label $sample_1 save_ save_3D_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_2 save_ save_3D_HCH-SFNOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCH-SFNOESY' _Sample_label $sample_1 save_ save_3D_CNH-SFNOESY_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CNH-SFNOESY' _Sample_label $sample_1 save_ save_3D_NCH-SFNOESY_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D NCH-SFNOESY' _Sample_label $sample_1 save_ save_3D_CCH-SFNOESY_8 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CCH-SFNOESY' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_9 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 100 . mM pH 7 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.000 internal indirect . . . 0.25144953 DSS H 1 'methyl protons' ppm 0.000 internal direct . . . 1.0 DSS N 15 'methyl protons' ppm 0.000 internal indirect . . . 0.10132912 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chemical_shifts_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' '2D 1H-13C HSQC' '2D 1H-13C HMQC' '3D HNCACB' '3D HCH-SFNOESY' '3D CNH-SFNOESY' '3D NCH-SFNOESY' '3D CCH-SFNOESY' '3D 1H-15N NOESY' stop_ loop_ _Sample_label $sample_2 $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name entity_1 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 96 1 ALA H H 8.471 0.00 . 2 96 1 ALA HB H 1.388 0.00 . 3 96 1 ALA CB C 19.346 0.00 . 4 96 1 ALA N N 125.679 0.00 . 5 97 2 ALA H H 8.438 0.00 . 6 97 2 ALA HB H 1.252 0.00 . 7 97 2 ALA CB C 19.071 0.00 . 8 97 2 ALA N N 123.903 0.00 . 9 98 3 LEU H H 8.746 0.00 . 10 98 3 LEU HD1 H 0.737 0.00 . 11 98 3 LEU HD2 H 0.945 0.00 . 12 98 3 LEU CD1 C 25.768 0.00 . 13 98 3 LEU CD2 C 23.465 0.00 . 14 98 3 LEU N N 122.975 0.00 . 15 99 4 VAL H H 8.173 0.00 . 16 99 4 VAL HG1 H 0.913 0.00 . 17 99 4 VAL CG1 C 20.913 0.00 . 18 99 4 VAL N N 121.546 0.00 . 19 100 5 ALA H H 8.308 0.00 . 20 100 5 ALA HB H 1.074 0.00 . 21 100 5 ALA CB C 21.856 0.00 . 22 100 5 ALA N N 127.288 0.00 . 23 101 6 HIS H H 8.678 0.00 . 24 101 6 HIS N N 118.819 0.00 . 25 102 7 VAL HG1 H 1.021 0.00 . 26 102 7 VAL HG2 H 1.006 0.00 . 27 102 7 VAL CG1 C 21.432 0.00 . 28 102 7 VAL CG2 C 21.982 0.00 . 29 103 8 THR H H 8.839 0.00 . 30 103 8 THR HG2 H 1.204 0.00 . 31 103 8 THR CG2 C 21.526 0.00 . 32 103 8 THR N N 119.803 0.00 . 33 116 21 GLY H H 8.697 0.01 . 34 116 21 GLY CA C 45.187 0.00 . 35 116 21 GLY N N 109.837 0.11 . 36 117 22 ARG H H 8.284 0.01 . 37 117 22 ARG CA C 55.590 0.01 . 38 117 22 ARG CB C 31.191 0.01 . 39 117 22 ARG N N 119.796 0.07 . 40 118 23 ASP H H 8.446 0.04 . 41 118 23 ASP CA C 54.960 0.08 . 42 118 23 ASP CB C 40.719 0.01 . 43 118 23 ASP N N 123.499 0.14 . 44 119 24 LEU H H 8.576 0.07 . 45 119 24 LEU HD1 H 0.791 0.00 . 46 119 24 LEU HD2 H 0.878 0.00 . 47 119 24 LEU CA C 53.296 0.03 . 48 119 24 LEU CB C 45.734 0.04 . 49 119 24 LEU CD1 C 24.401 0.03 . 50 119 24 LEU CD2 C 24.321 0.00 . 51 119 24 LEU N N 121.816 0.93 . 52 120 25 ARG H H 8.688 0.01 . 53 120 25 ARG CA C 54.385 0.00 . 54 120 25 ARG N N 123.115 1.11 . 55 121 26 ALA H H 8.920 0.06 . 56 121 26 ALA HB H 1.069 0.00 . 57 121 26 ALA CA C 51.042 0.00 . 58 121 26 ALA CB C 23.415 0.23 . 59 121 26 ALA N N 125.008 0.29 . 60 122 27 GLU H H 8.704 0.08 . 61 122 27 GLU CA C 55.967 0.00 . 62 122 27 GLU CB C 31.693 0.00 . 63 122 27 GLU N N 120.460 0.27 . 64 123 28 LEU H H 8.950 0.00 . 65 123 28 LEU HD1 H 0.789 0.00 . 66 123 28 LEU HD2 H 0.712 0.00 . 67 123 28 LEU CA C 50.883 0.00 . 68 123 28 LEU CB C 45.157 0.00 . 69 123 28 LEU CD1 C 24.059 0.01 . 70 123 28 LEU CD2 C 26.224 0.00 . 71 123 28 LEU N N 126.675 0.02 . 72 124 29 PRO CA C 61.121 0.00 . 73 124 29 PRO CB C 31.581 0.00 . 74 125 30 LEU H H 8.871 0.12 . 75 125 30 LEU HD1 H 0.705 0.00 . 76 125 30 LEU HD2 H 0.792 0.00 . 77 125 30 LEU CA C 53.036 0.00 . 78 125 30 LEU CB C 46.704 0.01 . 79 125 30 LEU CD1 C 27.545 0.00 . 80 125 30 LEU CD2 C 24.072 0.00 . 81 125 30 LEU N N 124.224 0.28 . 82 126 31 THR H H 9.003 0.00 . 83 126 31 THR HG2 H 1.440 0.00 . 84 126 31 THR CA C 60.867 0.02 . 85 126 31 THR CB C 71.023 0.01 . 86 126 31 THR CG2 C 22.495 0.02 . 87 126 31 THR N N 111.868 0.09 . 88 127 32 LEU H H 9.125 0.40 . 89 127 32 LEU HD1 H 0.781 0.00 . 90 127 32 LEU HD2 H 0.821 0.00 . 91 127 32 LEU CA C 58.786 0.02 . 92 127 32 LEU CB C 41.422 0.02 . 93 127 32 LEU CD1 C 24.869 0.00 . 94 127 32 LEU CD2 C 25.883 0.00 . 95 127 32 LEU N N 120.347 0.19 . 96 128 33 GLU H H 8.921 0.48 . 97 128 33 GLU CA C 60.323 0.05 . 98 128 33 GLU CB C 29.652 0.03 . 99 128 33 GLU N N 118.299 0.54 . 100 129 34 GLU H H 7.841 0.07 . 101 129 34 GLU CA C 59.084 0.00 . 102 129 34 GLU CB C 31.144 0.04 . 103 129 34 GLU N N 120.344 0.44 . 104 130 35 ALA H H 8.518 0.12 . 105 130 35 ALA HB H 1.693 0.00 . 106 130 35 ALA CA C 54.336 0.00 . 107 130 35 ALA CB C 18.761 0.20 . 108 130 35 ALA N N 120.657 0.83 . 109 131 36 PHE H H 8.086 0.28 . 110 131 36 PHE CA C 61.471 0.00 . 111 131 36 PHE CB C 39.593 0.01 . 112 131 36 PHE N N 116.094 0.43 . 113 132 37 HIS H H 8.017 0.05 . 114 132 37 HIS CA C 58.153 0.00 . 115 132 37 HIS CB C 31.181 0.03 . 116 132 37 HIS N N 113.781 0.33 . 117 133 38 GLY H H 8.203 0.00 . 118 133 38 GLY CA C 44.810 0.00 . 119 133 38 GLY N N 107.710 0.07 . 120 134 39 GLY H H 8.687 0.00 . 121 134 39 GLY CA C 44.786 0.00 . 122 134 39 GLY N N 107.498 0.00 . 123 135 40 GLU H H 8.523 0.00 . 124 135 40 GLU CA C 55.725 0.11 . 125 135 40 GLU CB C 31.747 0.02 . 126 135 40 GLU N N 121.055 0.24 . 127 136 41 ARG H H 8.975 0.03 . 128 136 41 ARG CA C 54.156 0.05 . 129 136 41 ARG CB C 34.559 0.01 . 130 136 41 ARG N N 123.806 0.81 . 131 137 42 VAL H H 8.548 0.07 . 132 137 42 VAL HG2 H 0.907 0.00 . 133 137 42 VAL CA C 62.179 0.01 . 134 137 42 VAL CB C 31.996 0.03 . 135 137 42 VAL CG2 C 21.569 0.02 . 136 137 42 VAL N N 123.587 0.72 . 137 138 43 VAL H H 9.079 0.11 . 138 138 43 VAL HG1 H 0.792 0.00 . 139 138 43 VAL HG2 H 0.818 0.00 . 140 138 43 VAL CA C 58.335 0.02 . 141 138 43 VAL CB C 34.335 0.06 . 142 138 43 VAL CG1 C 20.244 0.00 . 143 138 43 VAL CG2 C 21.218 0.00 . 144 138 43 VAL N N 122.471 2.27 . 145 139 44 GLU H H 8.630 0.00 . 146 139 44 GLU CA C 54.587 0.08 . 147 139 44 GLU CB C 31.943 0.00 . 148 139 44 GLU N N 122.690 0.02 . 149 140 45 VAL H H 8.887 0.03 . 150 140 45 VAL HG1 H 0.759 0.00 . 151 140 45 VAL HG2 H 0.706 0.00 . 152 140 45 VAL CA C 60.390 0.00 . 153 140 45 VAL CB C 33.903 0.03 . 154 140 45 VAL CG1 C 19.348 0.04 . 155 140 45 VAL CG2 C 20.939 0.02 . 156 140 45 VAL N N 124.077 0.14 . 157 141 46 ALA H H 9.446 0.04 . 158 141 46 ALA HB H 1.361 0.00 . 159 141 46 ALA CA C 52.915 0.00 . 160 141 46 ALA CB C 17.551 0.01 . 161 141 46 ALA N N 130.925 0.16 . 162 142 47 GLY H H 8.701 0.03 . 163 142 47 GLY CA C 45.535 0.01 . 164 142 47 GLY N N 103.979 0.13 . 165 143 48 ARG H H 8.072 0.02 . 166 143 48 ARG CA C 54.962 0.08 . 167 143 48 ARG N N 121.212 0.01 . 168 144 49 ARG H H 8.507 0.01 . 169 144 49 ARG CA C 55.780 0.04 . 170 144 49 ARG CB C 31.202 0.00 . 171 144 49 ARG N N 122.905 0.58 . 172 145 50 VAL H H 9.196 0.01 . 173 145 50 VAL HG1 H 0.775 0.00 . 174 145 50 VAL CA C 60.740 0.07 . 175 145 50 VAL CB C 35.300 0.07 . 176 145 50 VAL CG1 C 21.534 0.00 . 177 145 50 VAL N N 124.227 0.67 . 178 146 51 SER H H 8.593 0.02 . 179 146 51 SER CA C 58.276 0.01 . 180 146 51 SER CB C 63.121 0.01 . 181 146 51 SER N N 121.820 0.14 . 182 147 52 VAL H H 9.111 0.06 . 183 147 52 VAL HG2 H 0.693 0.00 . 184 147 52 VAL CA C 60.279 0.03 . 185 147 52 VAL CB C 34.969 0.02 . 186 147 52 VAL CG2 C 21.162 0.00 . 187 147 52 VAL N N 124.710 0.19 . 188 148 53 ARG H H 8.626 0.01 . 189 148 53 ARG CA C 55.093 0.00 . 190 148 53 ARG CB C 30.112 0.01 . 191 148 53 ARG N N 125.585 0.26 . 192 149 54 ILE H H 8.933 0.09 . 193 149 54 ILE HD1 H 0.580 0.00 . 194 149 54 ILE CA C 57.942 0.00 . 195 149 54 ILE CB C 38.740 0.00 . 196 149 54 ILE CD1 C 12.776 0.00 . 197 149 54 ILE N N 128.781 0.11 . 198 151 56 PRO CA C 62.963 0.00 . 199 151 56 PRO CB C 31.585 0.00 . 200 152 57 GLY H H 8.484 0.15 . 201 152 57 GLY CA C 46.355 0.01 . 202 152 57 GLY N N 111.709 0.03 . 203 153 58 VAL H H 7.207 0.00 . 204 153 58 VAL HG1 H 1.007 0.00 . 205 153 58 VAL HG2 H 1.080 0.00 . 206 153 58 VAL CA C 62.198 0.00 . 207 153 58 VAL CB C 32.550 0.00 . 208 153 58 VAL CG1 C 19.823 0.01 . 209 153 58 VAL CG2 C 21.722 0.00 . 210 153 58 VAL N N 115.036 0.57 . 211 154 59 ARG H H 8.058 0.17 . 212 154 59 ARG CA C 53.743 0.00 . 213 154 59 ARG CB C 32.954 0.06 . 214 154 59 ARG N N 117.135 1.50 . 215 155 60 GLU H H 8.294 0.17 . 216 155 60 GLU CA C 58.505 0.02 . 217 155 60 GLU CB C 29.290 0.01 . 218 155 60 GLU N N 120.632 0.44 . 219 156 61 GLY H H 9.316 0.05 . 220 156 61 GLY CA C 45.378 0.01 . 221 156 61 GLY N N 117.059 0.14 . 222 157 62 SER H H 8.280 0.00 . 223 157 62 SER CA C 61.377 0.00 . 224 157 62 SER CB C 63.493 0.00 . 225 157 62 SER N N 118.583 0.03 . 226 158 63 VAL H H 8.432 0.02 . 227 158 63 VAL HG1 H 0.738 0.00 . 228 158 63 VAL HG2 H 0.968 0.00 . 229 158 63 VAL CA C 61.113 0.00 . 230 158 63 VAL CB C 33.806 0.01 . 231 158 63 VAL CG1 C 21.609 0.00 . 232 158 63 VAL CG2 C 21.521 0.00 . 233 158 63 VAL N N 122.029 0.13 . 234 159 64 ILE H H 9.609 0.02 . 235 159 64 ILE HD1 H 0.748 0.00 . 236 159 64 ILE CA C 60.617 0.07 . 237 159 64 ILE CB C 40.316 0.00 . 238 159 64 ILE CD1 C 14.937 0.00 . 239 159 64 ILE N N 130.784 0.09 . 240 160 65 ARG H H 8.811 0.00 . 241 160 65 ARG CA C 55.291 0.00 . 242 160 65 ARG CB C 31.973 0.00 . 243 160 65 ARG N N 128.615 0.06 . 244 161 66 VAL H H 9.834 0.01 . 245 161 66 VAL HG1 H 0.836 0.00 . 246 161 66 VAL HG2 H 0.922 0.00 . 247 161 66 VAL CA C 59.477 0.00 . 248 161 66 VAL CB C 31.909 0.00 . 249 161 66 VAL CG1 C 20.260 0.00 . 250 161 66 VAL CG2 C 22.139 0.00 . 251 161 66 VAL N N 130.365 0.17 . 252 162 67 PRO CA C 63.164 0.00 . 253 162 67 PRO CB C 32.216 0.00 . 254 163 68 GLY H H 9.450 0.02 . 255 163 68 GLY CA C 47.020 0.01 . 256 163 68 GLY N N 110.794 0.10 . 257 164 69 MET H H 6.592 0.00 . 258 164 69 MET HE H 2.094 0.00 . 259 164 69 MET CA C 53.612 0.00 . 260 164 69 MET CB C 31.881 0.00 . 261 164 69 MET CE C 17.927 0.00 . 262 164 69 MET N N 115.504 0.01 . 263 165 70 GLY H H 8.217 0.05 . 264 165 70 GLY CA C 44.074 0.01 . 265 165 70 GLY N N 109.061 0.18 . 266 166 71 GLY H H 8.799 0.00 . 267 166 71 GLY CA C 45.671 0.01 . 268 166 71 GLY N N 111.320 0.00 . 269 167 72 GLN H H 8.498 0.02 . 270 167 72 GLN CA C 56.487 0.01 . 271 167 72 GLN CB C 29.084 0.00 . 272 167 72 GLN N N 120.638 0.14 . 273 168 73 GLY H H 8.007 0.02 . 274 168 73 GLY CA C 44.762 0.00 . 275 168 73 GLY N N 108.568 0.09 . 276 169 74 ASN H H 8.133 0.00 . 277 169 74 ASN CA C 49.614 0.00 . 278 169 74 ASN CB C 39.836 0.00 . 279 169 74 ASN N N 116.860 0.01 . 280 171 76 PRO CA C 62.675 0.00 . 281 171 76 PRO CB C 32.481 0.00 . 282 172 77 GLY H H 8.609 0.00 . 283 172 77 GLY CA C 43.874 0.05 . 284 172 77 GLY N N 106.469 0.05 . 285 173 78 ASP H H 9.155 0.02 . 286 173 78 ASP CA C 53.389 0.07 . 287 173 78 ASP CB C 42.858 0.05 . 288 173 78 ASP N N 118.650 0.03 . 289 174 79 LEU H H 7.853 0.01 . 290 174 79 LEU HD1 H 0.721 0.00 . 291 174 79 LEU HD2 H 0.740 0.00 . 292 174 79 LEU CA C 53.357 0.00 . 293 174 79 LEU CB C 44.763 0.00 . 294 174 79 LEU CD1 C 26.659 0.00 . 295 174 79 LEU CD2 C 22.886 0.00 . 296 174 79 LEU N N 121.089 0.14 . 297 175 80 LEU H H 9.392 0.01 . 298 175 80 LEU HD1 H 0.728 0.00 . 299 175 80 LEU HD2 H 0.790 0.00 . 300 175 80 LEU CA C 53.409 0.00 . 301 175 80 LEU CB C 41.965 0.00 . 302 175 80 LEU CD1 C 23.700 0.00 . 303 175 80 LEU CD2 C 24.816 0.00 . 304 175 80 LEU N N 129.463 0.04 . 305 176 81 LEU H H 8.902 0.03 . 306 176 81 LEU HD1 H 0.762 0.00 . 307 176 81 LEU HD2 H 0.693 0.00 . 308 176 81 LEU CA C 52.466 0.00 . 309 176 81 LEU CB C 42.755 0.11 . 310 176 81 LEU CD1 C 27.188 0.00 . 311 176 81 LEU CD2 C 24.578 0.01 . 312 176 81 LEU N N 123.034 0.06 . 313 177 82 VAL H H 8.868 0.05 . 314 177 82 VAL HG1 H 0.845 0.00 . 315 177 82 VAL CA C 61.034 0.03 . 316 177 82 VAL CB C 32.950 0.00 . 317 177 82 VAL CG1 C 20.423 0.01 . 318 177 82 VAL N N 123.798 0.04 . 319 178 83 VAL H H 9.060 0.00 . 320 178 83 VAL HG1 H 0.921 0.00 . 321 178 83 VAL HG2 H 0.679 0.00 . 322 178 83 VAL CA C 64.471 0.00 . 323 178 83 VAL CB C 31.856 0.03 . 324 178 83 VAL CG1 C 22.092 0.00 . 325 178 83 VAL CG2 C 21.595 0.01 . 326 178 83 VAL N N 129.734 0.04 . 327 179 84 ARG H H 8.244 0.05 . 328 179 84 ARG CA C 53.290 0.02 . 329 179 84 ARG CB C 32.246 0.08 . 330 179 84 ARG N N 130.280 0.62 . 331 180 85 LEU H H 9.105 0.03 . 332 180 85 LEU HD1 H 0.850 0.00 . 333 180 85 LEU HD2 H 0.686 0.00 . 334 180 85 LEU CA C 53.605 0.00 . 335 180 85 LEU CB C 41.611 0.00 . 336 180 85 LEU CD1 C 25.984 0.00 . 337 180 85 LEU CD2 C 23.514 0.01 . 338 180 85 LEU N N 124.902 0.45 . 339 181 86 LEU H H 8.789 0.09 . 340 181 86 LEU HD1 H 0.953 0.00 . 341 181 86 LEU HD2 H 0.885 0.00 . 342 181 86 LEU CA C 52.989 0.00 . 343 181 86 LEU CB C 41.816 0.00 . 344 181 86 LEU CD1 C 22.604 0.00 . 345 181 86 LEU CD2 C 25.435 0.01 . 346 181 86 LEU N N 127.089 0.39 . 347 183 88 HIS H H 9.174 0.00 . 348 183 88 HIS N N 128.754 0.00 . 349 185 90 VAL H H 10.243 0.00 . 350 185 90 VAL HG1 H 0.833 0.00 . 351 185 90 VAL HG2 H 0.114 0.00 . 352 185 90 VAL CG1 C 21.161 0.00 . 353 185 90 VAL CG2 C 20.575 0.00 . 354 185 90 VAL N N 121.653 0.00 . 355 186 91 PHE H H 8.452 0.00 . 356 186 91 PHE N N 118.117 0.00 . 357 187 92 ARG H H 9.049 0.00 . 358 187 92 ARG N N 120.162 0.00 . 359 188 93 LEU H H 8.752 0.00 . 360 188 93 LEU HD1 H 0.171 0.00 . 361 188 93 LEU HD2 H 0.239 0.00 . 362 188 93 LEU CD1 C 25.737 0.00 . 363 188 93 LEU CD2 C 23.220 0.00 . 364 188 93 LEU N N 126.813 0.00 . 365 189 94 GLU H H 9.289 0.00 . 366 189 94 GLU N N 129.321 0.00 . 367 191 96 GLN H H 8.706 0.00 . 368 191 96 GLN CA C 56.368 0.00 . 369 191 96 GLN CB C 29.431 0.00 . 370 191 96 GLN N N 120.785 0.01 . 371 192 97 ASP H H 8.519 0.00 . 372 192 97 ASP N N 122.845 0.00 . 373 193 98 LEU H H 8.811 0.00 . 374 193 98 LEU HD1 H 0.614 0.00 . 375 193 98 LEU HD2 H 0.716 0.00 . 376 193 98 LEU CD1 C 25.892 0.00 . 377 193 98 LEU CD2 C 24.684 0.00 . 378 193 98 LEU N N 119.796 0.00 . 379 194 99 TYR H H 9.224 0.00 . 380 194 99 TYR N N 119.327 0.02 . 381 195 100 ALA H H 8.928 0.01 . 382 195 100 ALA HB H 1.278 0.00 . 383 195 100 ALA CA C 51.129 0.00 . 384 195 100 ALA CB C 23.523 0.54 . 385 195 100 ALA N N 124.286 0.86 . 386 196 101 THR H H 8.919 0.00 . 387 196 101 THR HG2 H 1.195 0.00 . 388 196 101 THR CA C 62.031 0.00 . 389 196 101 THR CB C 70.995 0.00 . 390 196 101 THR CG2 C 22.434 0.00 . 391 196 101 THR N N 116.757 0.00 . 392 197 102 LEU H H 8.755 0.06 . 393 197 102 LEU HD1 H 0.573 0.00 . 394 197 102 LEU HD2 H 0.938 0.00 . 395 197 102 LEU CA C 53.084 0.08 . 396 197 102 LEU CB C 47.282 0.01 . 397 197 102 LEU CD1 C 26.287 0.00 . 398 197 102 LEU CD2 C 23.565 0.00 . 399 197 102 LEU N N 126.971 0.78 . 400 198 103 ASP H H 8.625 0.01 . 401 198 103 ASP CA C 54.178 0.03 . 402 198 103 ASP CB C 40.782 0.00 . 403 198 103 ASP N N 129.669 0.01 . 404 199 104 VAL H H 9.249 0.00 . 405 199 104 VAL HG1 H 0.802 0.00 . 406 199 104 VAL HG2 H 0.854 0.00 . 407 199 104 VAL CA C 58.722 0.00 . 408 199 104 VAL CB C 35.010 0.00 . 409 199 104 VAL CG1 C 21.793 0.00 . 410 199 104 VAL CG2 C 22.917 0.00 . 411 199 104 VAL N N 124.840 0.05 . 412 200 105 PRO CA C 62.470 0.00 . 413 200 105 PRO CB C 33.261 0.00 . 414 201 106 ALA H H 9.022 0.00 . 415 201 106 ALA HB H 1.455 0.00 . 416 201 106 ALA CA C 57.166 0.00 . 417 201 106 ALA CB C 16.102 0.07 . 418 201 106 ALA N N 127.254 0.13 . 419 202 107 PRO CA C 66.199 0.00 . 420 202 107 PRO CB C 31.426 0.00 . 421 203 108 ILE H H 7.146 0.00 . 422 203 108 ILE HD1 H 0.790 0.00 . 423 203 108 ILE CA C 62.680 0.01 . 424 203 108 ILE CB C 36.685 0.01 . 425 203 108 ILE CD1 C 11.565 0.00 . 426 203 108 ILE N N 116.547 0.03 . 427 204 109 ALA H H 7.557 0.01 . 428 204 109 ALA HB H 1.378 0.00 . 429 204 109 ALA CA C 55.002 0.01 . 430 204 109 ALA CB C 17.365 0.06 . 431 204 109 ALA N N 121.792 0.09 . 432 205 110 VAL H H 7.323 0.01 . 433 205 110 VAL HG1 H 0.954 0.00 . 434 205 110 VAL HG2 H 1.071 0.00 . 435 205 110 VAL CA C 67.056 0.00 . 436 205 110 VAL CB C 32.262 0.09 . 437 205 110 VAL CG1 C 21.151 0.00 . 438 205 110 VAL CG2 C 22.270 0.00 . 439 205 110 VAL N N 111.973 0.03 . 440 206 111 VAL H H 7.198 0.00 . 441 206 111 VAL HG1 H 1.158 0.00 . 442 206 111 VAL HG2 H 1.074 0.00 . 443 206 111 VAL CA C 63.214 0.00 . 444 206 111 VAL CB C 33.263 0.04 . 445 206 111 VAL CG1 C 19.489 0.00 . 446 206 111 VAL CG2 C 21.840 0.00 . 447 206 111 VAL N N 113.292 0.09 . 448 207 112 GLY H H 8.434 0.00 . 449 207 112 GLY CA C 43.789 0.04 . 450 207 112 GLY N N 113.713 0.01 . 451 208 113 GLY H H 8.157 0.01 . 452 208 113 GLY CA C 45.576 0.00 . 453 208 113 GLY N N 104.840 0.06 . 454 209 114 LYS H H 8.435 0.01 . 455 209 114 LYS CA C 55.210 0.01 . 456 209 114 LYS CB C 35.003 0.07 . 457 209 114 LYS N N 120.873 0.01 . 458 210 115 VAL H H 8.701 0.01 . 459 210 115 VAL HG1 H 0.796 0.00 . 460 210 115 VAL HG2 H 1.005 0.00 . 461 210 115 VAL CA C 59.858 0.00 . 462 210 115 VAL CB C 35.671 0.06 . 463 210 115 VAL CG1 C 20.499 0.00 . 464 210 115 VAL CG2 C 22.074 0.00 . 465 210 115 VAL N N 117.504 0.37 . 466 211 116 ARG H H 8.519 0.01 . 467 211 116 ARG CA C 55.972 0.05 . 468 211 116 ARG CB C 31.508 0.01 . 469 211 116 ARG N N 123.421 0.19 . 470 212 117 ALA H H 9.053 0.03 . 471 212 117 ALA HB H 1.189 0.00 . 472 212 117 ALA CA C 50.325 0.05 . 473 212 117 ALA CB C 22.578 0.24 . 474 212 117 ALA N N 127.346 0.02 . 475 213 118 MET H H 8.514 0.03 . 476 213 118 MET HE H 2.123 0.00 . 477 213 118 MET CE C 16.379 0.00 . 478 213 118 MET N N 120.129 0.35 . 479 214 119 THR H H 7.356 0.00 . 480 214 119 THR HG2 H 1.041 0.00 . 481 214 119 THR CA C 59.866 0.00 . 482 214 119 THR CB C 72.145 0.00 . 483 214 119 THR CG2 C 21.490 0.00 . 484 214 119 THR N N 111.831 0.00 . 485 215 120 LEU H H 8.943 0.22 . 486 215 120 LEU HD1 H 0.220 0.00 . 487 215 120 LEU HD2 H 0.328 0.00 . 488 215 120 LEU CA C 57.928 0.03 . 489 215 120 LEU CB C 41.577 0.03 . 490 215 120 LEU CD1 C 26.061 0.00 . 491 215 120 LEU CD2 C 23.584 0.00 . 492 215 120 LEU N N 120.911 0.26 . 493 216 121 GLU H H 7.831 0.04 . 494 216 121 GLU CA C 56.293 0.00 . 495 216 121 GLU CB C 30.029 0.00 . 496 216 121 GLU N N 115.187 0.11 . 497 217 122 GLY H H 7.223 0.00 . 498 217 122 GLY N N 108.030 0.00 . 499 218 123 PRO CA C 62.922 0.00 . 500 218 123 PRO CB C 32.310 0.00 . 501 219 124 VAL H H 9.003 0.01 . 502 219 124 VAL HG1 H 0.714 0.00 . 503 219 124 VAL HG2 H 0.773 0.00 . 504 219 124 VAL CA C 60.028 0.01 . 505 219 124 VAL CB C 35.971 0.01 . 506 219 124 VAL CG1 C 20.045 0.00 . 507 219 124 VAL CG2 C 21.351 0.00 . 508 219 124 VAL N N 119.237 0.47 . 509 220 125 GLU H H 8.473 0.01 . 510 220 125 GLU CA C 56.050 0.01 . 511 220 125 GLU CB C 31.091 0.02 . 512 220 125 GLU N N 123.004 0.23 . 513 221 126 VAL H H 9.157 0.02 . 514 221 126 VAL HG1 H 0.695 0.00 . 515 221 126 VAL HG2 H 0.737 0.00 . 516 221 126 VAL CA C 60.913 0.00 . 517 221 126 VAL CB C 34.294 0.02 . 518 221 126 VAL CG1 C 21.185 0.00 . 519 221 126 VAL CG2 C 21.834 0.00 . 520 221 126 VAL N N 125.356 0.29 . 521 222 127 ALA H H 8.435 0.00 . 522 222 127 ALA HB H 1.256 0.00 . 523 222 127 ALA CA C 51.449 0.00 . 524 222 127 ALA CB C 18.485 0.02 . 525 222 127 ALA N N 128.245 0.26 . 526 223 128 VAL H H 8.956 0.01 . 527 223 128 VAL HG1 H 0.913 0.00 . 528 223 128 VAL HG2 H 0.790 0.00 . 529 223 128 VAL CA C 59.449 0.00 . 530 223 128 VAL CB C 32.763 0.00 . 531 223 128 VAL CG1 C 20.937 0.00 . 532 223 128 VAL CG2 C 21.697 0.00 . 533 223 128 VAL N N 125.897 0.02 . 534 225 130 PRO CA C 63.113 0.00 . 535 225 130 PRO CB C 32.180 0.00 . 536 226 131 ARG H H 8.359 0.00 . 537 226 131 ARG CA C 56.814 0.00 . 538 226 131 ARG CB C 26.461 0.01 . 539 226 131 ARG N N 117.280 0.02 . 540 227 132 THR H H 8.540 0.01 . 541 227 132 THR HG2 H 1.109 0.00 . 542 227 132 THR CA C 66.050 0.02 . 543 227 132 THR CB C 69.282 0.00 . 544 227 132 THR CG2 C 23.221 0.00 . 545 227 132 THR N N 120.079 0.02 . 546 228 133 GLN H H 8.189 0.00 . 547 228 133 GLN CA C 53.511 0.00 . 548 228 133 GLN CB C 31.505 0.01 . 549 228 133 GLN N N 125.362 0.08 . 550 229 134 ALA H H 8.543 0.00 . 551 229 134 ALA HB H 1.467 0.00 . 552 229 134 ALA CA C 53.656 0.03 . 553 229 134 ALA CB C 18.551 0.05 . 554 229 134 ALA N N 121.867 0.12 . 555 230 135 GLY H H 9.135 0.01 . 556 230 135 GLY CA C 45.016 0.00 . 557 230 135 GLY N N 111.186 0.03 . 558 231 136 ARG H H 7.623 0.01 . 559 231 136 ARG CA C 56.832 0.02 . 560 231 136 ARG CB C 30.753 0.02 . 561 231 136 ARG N N 121.867 0.01 . 562 232 137 LYS H H 8.732 0.01 . 563 232 137 LYS CA C 54.897 0.04 . 564 232 137 LYS CB C 33.861 0.00 . 565 232 137 LYS N N 124.074 0.07 . 566 233 138 LEU H H 9.373 0.04 . 567 233 138 LEU HD1 H 0.749 0.00 . 568 233 138 LEU HD2 H 0.830 0.00 . 569 233 138 LEU CA C 53.656 0.00 . 570 233 138 LEU CB C 42.475 0.10 . 571 233 138 LEU CD1 C 25.825 0.00 . 572 233 138 LEU CD2 C 22.627 0.00 . 573 233 138 LEU N N 126.843 0.11 . 574 234 139 ARG H H 8.590 0.01 . 575 234 139 ARG CA C 55.169 0.00 . 576 234 139 ARG CB C 32.772 0.01 . 577 234 139 ARG N N 123.476 0.07 . 578 235 140 LEU H H 9.485 0.03 . 579 235 140 LEU HD1 H 0.776 0.00 . 580 235 140 LEU HD2 H 0.713 0.00 . 581 235 140 LEU CA C 53.820 0.01 . 582 235 140 LEU CB C 41.306 0.03 . 583 235 140 LEU CD1 C 24.306 0.00 . 584 235 140 LEU CD2 C 24.914 0.00 . 585 235 140 LEU N N 130.715 0.04 . 586 236 141 LYS H H 8.589 0.00 . 587 236 141 LYS CA C 56.768 0.00 . 588 236 141 LYS CB C 32.226 0.02 . 589 236 141 LYS N N 126.807 0.04 . 590 237 142 GLY H H 9.355 0.04 . 591 237 142 GLY CA C 47.050 0.02 . 592 237 142 GLY N N 113.082 0.12 . 593 238 143 LYS H H 6.549 0.03 . 594 238 143 LYS CA C 54.015 0.03 . 595 238 143 LYS CB C 32.068 0.01 . 596 238 143 LYS N N 114.309 0.07 . 597 239 144 GLY H H 8.197 0.01 . 598 239 144 GLY CA C 43.462 0.00 . 599 239 144 GLY N N 107.864 0.20 . 600 240 145 PHE H H 9.088 0.00 . 601 240 145 PHE N N 125.499 0.00 . 602 241 146 PRO CA C 63.357 0.00 . 603 241 146 PRO CB C 32.373 0.00 . 604 242 147 GLY H H 7.820 0.04 . 605 242 147 GLY CA C 45.032 0.00 . 606 242 147 GLY N N 109.540 0.37 . 607 243 148 PRO CA C 64.945 0.00 . 608 243 148 PRO CB C 31.999 0.00 . 609 244 149 ALA H H 8.608 0.02 . 610 244 149 ALA HB H 1.332 0.00 . 611 244 149 ALA CA C 51.284 0.02 . 612 244 149 ALA CB C 18.950 0.06 . 613 244 149 ALA N N 119.997 0.04 . 614 245 150 GLY H H 7.522 0.01 . 615 245 150 GLY CA C 44.189 0.02 . 616 245 150 GLY N N 108.044 0.03 . 617 246 151 ARG H H 8.500 0.02 . 618 246 151 ARG CA C 56.054 0.00 . 619 246 151 ARG N N 119.899 0.39 . 620 247 152 GLY H H 8.105 0.00 . 621 247 152 GLY N N 107.086 0.00 . 622 248 153 ASP H H 9.283 0.10 . 623 248 153 ASP CA C 53.749 0.05 . 624 248 153 ASP CB C 43.984 0.11 . 625 248 153 ASP N N 119.505 0.21 . 626 249 154 LEU H H 7.645 0.03 . 627 249 154 LEU HD1 H 0.856 0.00 . 628 249 154 LEU HD2 H 0.825 0.00 . 629 249 154 LEU CA C 52.845 0.00 . 630 249 154 LEU CB C 45.796 0.02 . 631 249 154 LEU CD1 C 26.688 0.00 . 632 249 154 LEU CD2 C 24.082 0.00 . 633 249 154 LEU N N 121.069 0.38 . 634 250 155 TYR H H 9.257 0.08 . 635 250 155 TYR CA C 57.106 0.01 . 636 250 155 TYR CB C 41.017 0.03 . 637 250 155 TYR N N 125.727 0.08 . 638 251 156 LEU H H 9.032 0.01 . 639 251 156 LEU HD1 H 0.813 0.00 . 640 251 156 LEU HD2 H 0.763 0.00 . 641 251 156 LEU CA C 51.974 0.00 . 642 251 156 LEU CB C 43.958 0.00 . 643 251 156 LEU CD1 C 24.020 0.00 . 644 251 156 LEU CD2 C 27.237 0.00 . 645 251 156 LEU N N 122.526 0.05 . 646 252 157 GLU H H 9.036 0.03 . 647 252 157 GLU CA C 55.128 0.06 . 648 252 157 GLU CB C 31.265 0.03 . 649 252 157 GLU N N 124.517 0.11 . 650 253 158 VAL H H 8.988 0.01 . 651 253 158 VAL HG1 H 0.685 0.00 . 652 253 158 VAL HG2 H 0.981 0.00 . 653 253 158 VAL CA C 64.796 0.01 . 654 253 158 VAL CB C 32.320 0.00 . 655 253 158 VAL CG1 C 21.166 0.00 . 656 253 158 VAL CG2 C 22.226 0.00 . 657 253 158 VAL N N 128.139 0.21 . 658 254 159 ARG H H 9.375 0.01 . 659 254 159 ARG CA C 55.166 0.01 . 660 254 159 ARG CB C 32.474 0.02 . 661 254 159 ARG N N 133.724 0.16 . 662 255 160 ILE H H 8.382 0.00 . 663 255 160 ILE HD1 H 0.610 0.00 . 664 255 160 ILE CA C 59.250 0.01 . 665 255 160 ILE CB C 36.797 0.02 . 666 255 160 ILE CD1 C 10.924 0.00 . 667 255 160 ILE N N 126.118 0.14 . 668 256 161 THR H H 8.419 0.00 . 669 256 161 THR HG2 H 0.941 0.00 . 670 256 161 THR CA C 62.254 0.00 . 671 256 161 THR CB C 70.330 0.00 . 672 256 161 THR CG2 C 22.132 0.00 . 673 256 161 THR N N 126.970 0.06 . stop_ save_