data_27999 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Structural basis for client recognition and activity of Hsp40 chaperones ; _BMRB_accession_number 27999 _BMRB_flat_file_name bmr27999.str _Entry_type original _Submission_date 2019-08-16 _Accession_date 2019-08-16 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jiang Yajun . . 2 Rossi Paolo . . 3 Kalodimos Charalampos G. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 138 "15N chemical shifts" 138 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2019-11-07 original BMRB . stop_ loop_ _Related_BMRB_accession_number _Relationship 27998 'DnaJB1 SBD' 28000 'Ydj1 SBD' 28001 'CbpA SBD' stop_ _Original_release_date 2019-08-19 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structural basis for client recognition and activity of Hsp40 chaperones ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 31604242 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Jiang Yajun . . 2 Rossi Paolo . . 3 Kalodimos Charalampos G. . stop_ _Journal_abbreviation Science _Journal_volume 365 _Journal_issue 6459 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1313 _Page_last 1319 _Year 2019 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name Sis1_SBD _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label Sis1_SBD $Sis1_SBD stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Sis1_SBD _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Sis1_SBD _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function chaperone stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 158 _Mol_residue_sequence ; EEETVQVNLPVSLEDLFVGK KKSFKIGRKGPHGASEKTQI DIQLKPGWKAGTKITYKNQG DYNPQTGRRKTLQFVIQEKS HPNFKRDGDDLIYTLPLSFK ESLLGFSKTIQTIDGRTLPL SRVQPVQPSQTSTYPGQGMP TPKNPSQRGNLIVKYKVD ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 178 GLU 2 179 GLU 3 180 GLU 4 181 THR 5 182 VAL 6 183 GLN 7 184 VAL 8 185 ASN 9 186 LEU 10 187 PRO 11 188 VAL 12 189 SER 13 190 LEU 14 191 GLU 15 192 ASP 16 193 LEU 17 194 PHE 18 195 VAL 19 196 GLY 20 197 LYS 21 198 LYS 22 199 LYS 23 200 SER 24 201 PHE 25 202 LYS 26 203 ILE 27 204 GLY 28 205 ARG 29 206 LYS 30 207 GLY 31 208 PRO 32 209 HIS 33 210 GLY 34 211 ALA 35 212 SER 36 213 GLU 37 214 LYS 38 215 THR 39 216 GLN 40 217 ILE 41 218 ASP 42 219 ILE 43 220 GLN 44 221 LEU 45 222 LYS 46 223 PRO 47 224 GLY 48 225 TRP 49 226 LYS 50 227 ALA 51 228 GLY 52 229 THR 53 230 LYS 54 231 ILE 55 232 THR 56 233 TYR 57 234 LYS 58 235 ASN 59 236 GLN 60 237 GLY 61 238 ASP 62 239 TYR 63 240 ASN 64 241 PRO 65 242 GLN 66 243 THR 67 244 GLY 68 245 ARG 69 246 ARG 70 247 LYS 71 248 THR 72 249 LEU 73 250 GLN 74 251 PHE 75 252 VAL 76 253 ILE 77 254 GLN 78 255 GLU 79 256 LYS 80 257 SER 81 258 HIS 82 259 PRO 83 260 ASN 84 261 PHE 85 262 LYS 86 263 ARG 87 264 ASP 88 265 GLY 89 266 ASP 90 267 ASP 91 268 LEU 92 269 ILE 93 270 TYR 94 271 THR 95 272 LEU 96 273 PRO 97 274 LEU 98 275 SER 99 276 PHE 100 277 LYS 101 278 GLU 102 279 SER 103 280 LEU 104 281 LEU 105 282 GLY 106 283 PHE 107 284 SER 108 285 LYS 109 286 THR 110 287 ILE 111 288 GLN 112 289 THR 113 290 ILE 114 291 ASP 115 292 GLY 116 293 ARG 117 294 THR 118 295 LEU 119 296 PRO 120 297 LEU 121 298 SER 122 299 ARG 123 300 VAL 124 301 GLN 125 302 PRO 126 303 VAL 127 304 GLN 128 305 PRO 129 306 SER 130 307 GLN 131 308 THR 132 309 SER 133 310 THR 134 311 TYR 135 312 PRO 136 313 GLY 137 314 GLN 138 315 GLY 139 316 MET 140 317 PRO 141 318 THR 142 319 PRO 143 320 LYS 144 321 ASN 145 322 PRO 146 323 SER 147 324 GLN 148 325 ARG 149 326 GLY 150 327 ASN 151 328 LEU 152 329 ILE 153 330 VAL 154 331 LYS 155 332 TYR 156 333 LYS 157 334 VAL 158 335 ASP stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Sis1_SBD "baker's yeast" 4932 Eukaryota Fungi Saccharomyces cerevisiae stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Sis1_SBD 'recombinant technology' . Escherichia coli . pet16b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Sis1_SBD 700 uM '[U-100% 13C; U-100% 15N]' 'potassium chloride' 75 mM 'natural abundance' 'potassium phosphate' 20 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_PINE _Saveframe_category software _Name PINE _Version . loop_ _Vendor _Address _Electronic_address 'Bahrami, Markley, Assadi, and Eghbalnia' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCACB_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HNCO_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_1H-15N_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ save_3D_HNCACO_6 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACO' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.1 . M pH 7 . pH pressure 1 . atm temperature 305 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 DSS P 31 'methyl protons' ppm 0.00 na indirect . . . 0.404808636 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name Sis1_SBD _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 178 1 GLU H H 8.357 0.00 . 2 178 1 GLU N N 122.434 0.00 . 3 179 2 GLU H H 8.352 0.00 . 4 179 2 GLU N N 121.523 0.00 . 5 180 3 GLU H H 8.839 0.00 . 6 180 3 GLU N N 123.089 0.00 . 7 181 4 THR H H 8.293 0.00 . 8 181 4 THR N N 118.201 0.00 . 9 182 5 VAL H H 8.927 0.00 . 10 182 5 VAL N N 129.053 0.00 . 11 183 6 GLN H H 8.520 0.00 . 12 183 6 GLN N N 126.059 0.00 . 13 184 7 VAL H H 8.710 0.00 . 14 184 7 VAL N N 125.697 0.00 . 15 185 8 ASN H H 8.803 0.00 . 16 185 8 ASN N N 126.010 0.00 . 17 186 9 LEU H H 8.963 0.00 . 18 186 9 LEU N N 124.675 0.00 . 19 188 11 VAL H H 9.139 0.00 . 20 188 11 VAL N N 119.609 0.00 . 21 189 12 SER H H 9.278 0.00 . 22 189 12 SER N N 118.476 0.00 . 23 190 13 LEU H H 9.692 0.00 . 24 190 13 LEU N N 119.456 0.00 . 25 191 14 GLU H H 9.793 0.00 . 26 191 14 GLU N N 119.760 0.00 . 27 192 15 ASP H H 8.237 0.00 . 28 192 15 ASP N N 120.930 0.00 . 29 193 16 LEU H H 7.689 0.00 . 30 193 16 LEU N N 119.313 0.00 . 31 194 17 PHE H H 8.723 0.00 . 32 194 17 PHE N N 122.908 0.00 . 33 195 18 VAL H H 7.597 0.00 . 34 195 18 VAL N N 109.844 0.00 . 35 196 19 GLY H H 7.531 0.00 . 36 196 19 GLY N N 109.473 0.00 . 37 197 20 LYS H H 7.394 0.00 . 38 197 20 LYS N N 120.643 0.00 . 39 198 21 LYS H H 8.256 0.00 . 40 198 21 LYS N N 122.620 0.00 . 41 199 22 LYS H H 9.058 0.00 . 42 199 22 LYS N N 127.531 0.00 . 43 200 23 SER H H 8.493 0.00 . 44 200 23 SER N N 118.683 0.00 . 45 201 24 PHE H H 8.839 0.00 . 46 201 24 PHE N N 125.213 0.00 . 47 202 25 LYS H H 8.389 0.00 . 48 202 25 LYS N N 122.344 0.00 . 49 203 26 ILE H H 8.723 0.00 . 50 203 26 ILE N N 121.036 0.00 . 51 204 27 GLY H H 8.498 0.00 . 52 204 27 GLY N N 112.202 0.00 . 53 205 28 ARG H H 8.745 0.00 . 54 205 28 ARG N N 118.960 0.00 . 55 206 29 LYS H H 9.052 0.00 . 56 206 29 LYS N N 120.869 0.00 . 57 207 30 GLY H H 8.997 0.00 . 58 207 30 GLY N N 112.212 0.00 . 59 210 33 GLY H H 8.041 0.00 . 60 210 33 GLY N N 106.662 0.00 . 61 211 34 ALA H H 7.214 0.00 . 62 211 34 ALA N N 122.804 0.00 . 63 213 36 GLU H H 8.960 0.00 . 64 213 36 GLU N N 124.688 0.00 . 65 214 37 LYS H H 8.499 0.00 . 66 214 37 LYS N N 120.723 0.00 . 67 215 38 THR H H 8.740 0.00 . 68 215 38 THR N N 117.559 0.00 . 69 216 39 GLN H H 8.226 0.00 . 70 216 39 GLN N N 125.649 0.00 . 71 217 40 ILE H H 8.921 0.00 . 72 217 40 ILE N N 128.027 0.00 . 73 218 41 ASP H H 8.142 0.00 . 74 218 41 ASP N N 125.338 0.00 . 75 219 42 ILE H H 9.158 0.00 . 76 219 42 ILE N N 124.713 0.00 . 77 220 43 GLN H H 8.790 0.00 . 78 220 43 GLN N N 128.384 0.00 . 79 221 44 LEU H H 7.446 0.00 . 80 221 44 LEU N N 126.448 0.00 . 81 224 47 GLY H H 8.924 0.00 . 82 224 47 GLY N N 108.403 0.00 . 83 225 48 TRP H H 7.804 0.00 . 84 225 48 TRP HE1 H 10.286 0.00 . 85 225 48 TRP N N 120.866 0.00 . 86 225 48 TRP NE1 N 128.485 0.00 . 87 226 49 LYS H H 7.755 0.00 . 88 226 49 LYS N N 116.420 0.00 . 89 227 50 ALA H H 9.002 0.00 . 90 227 50 ALA N N 123.923 0.00 . 91 228 51 GLY H H 9.275 0.00 . 92 228 51 GLY N N 111.561 0.00 . 93 229 52 THR H H 7.595 0.00 . 94 229 52 THR N N 119.332 0.00 . 95 230 53 LYS H H 8.212 0.00 . 96 230 53 LYS N N 125.657 0.00 . 97 231 54 ILE H H 9.116 0.00 . 98 231 54 ILE N N 124.134 0.00 . 99 232 55 THR H H 8.691 0.00 . 100 232 55 THR N N 124.706 0.00 . 101 233 56 TYR H H 9.700 0.00 . 102 233 56 TYR N N 128.912 0.00 . 103 234 57 LYS H H 8.868 0.00 . 104 234 57 LYS N N 125.044 0.00 . 105 235 58 ASN H H 9.164 0.00 . 106 235 58 ASN N N 117.379 0.00 . 107 237 60 GLY H H 8.946 0.00 . 108 237 60 GLY N N 107.848 0.00 . 109 238 61 ASP H H 9.006 0.00 . 110 238 61 ASP N N 122.060 0.00 . 111 239 62 TYR H H 8.182 0.00 . 112 239 62 TYR N N 121.342 0.00 . 113 240 63 ASN H H 8.130 0.00 . 114 240 63 ASN N N 128.288 0.00 . 115 242 65 GLN H H 7.882 0.00 . 116 242 65 GLN N N 114.745 0.00 . 117 243 66 THR H H 6.830 0.00 . 118 243 66 THR N N 104.812 0.00 . 119 244 67 GLY H H 8.395 0.00 . 120 244 67 GLY N N 110.714 0.00 . 121 246 69 ARG H H 8.241 0.00 . 122 246 69 ARG N N 117.706 0.00 . 123 247 70 LYS H H 8.062 0.00 . 124 247 70 LYS N N 118.113 0.00 . 125 248 71 THR H H 8.740 0.00 . 126 248 71 THR N N 120.209 0.00 . 127 249 72 LEU H H 8.222 0.00 . 128 249 72 LEU N N 127.000 0.00 . 129 250 73 GLN H H 8.837 0.00 . 130 250 73 GLN N N 124.777 0.00 . 131 251 74 PHE H H 9.309 0.00 . 132 251 74 PHE N N 126.514 0.00 . 133 252 75 VAL H H 8.631 0.00 . 134 252 75 VAL N N 122.181 0.00 . 135 253 76 ILE H H 8.725 0.00 . 136 253 76 ILE N N 126.832 0.00 . 137 254 77 GLN H H 8.472 0.00 . 138 254 77 GLN N N 128.767 0.00 . 139 255 78 GLU H H 8.809 0.00 . 140 255 78 GLU N N 123.127 0.00 . 141 256 79 LYS H H 8.872 0.00 . 142 256 79 LYS N N 126.296 0.00 . 143 258 81 HIS H H 9.195 0.00 . 144 258 81 HIS N N 131.208 0.00 . 145 260 83 ASN H H 10.173 0.00 . 146 260 83 ASN N N 115.764 0.00 . 147 261 84 PHE H H 8.331 0.00 . 148 261 84 PHE N N 117.691 0.00 . 149 262 85 LYS H H 8.670 0.00 . 150 262 85 LYS N N 119.823 0.00 . 151 263 86 ARG H H 9.149 0.00 . 152 263 86 ARG N N 125.353 0.00 . 153 264 87 ASP H H 8.720 0.00 . 154 264 87 ASP N N 128.931 0.00 . 155 265 88 GLY H H 9.062 0.00 . 156 265 88 GLY N N 115.059 0.00 . 157 266 89 ASP H H 9.222 0.00 . 158 266 89 ASP N N 128.201 0.00 . 159 267 90 ASP H H 8.311 0.00 . 160 267 90 ASP N N 121.860 0.00 . 161 268 91 LEU H H 8.632 0.00 . 162 268 91 LEU N N 117.791 0.00 . 163 269 92 ILE H H 9.391 0.00 . 164 269 92 ILE N N 121.665 0.00 . 165 270 93 TYR H H 9.643 0.00 . 166 270 93 TYR N N 132.020 0.00 . 167 271 94 THR H H 8.103 0.00 . 168 271 94 THR N N 125.015 0.00 . 169 272 95 LEU H H 8.992 0.00 . 170 272 95 LEU N N 131.580 0.00 . 171 274 97 LEU H H 8.728 0.00 . 172 274 97 LEU N N 123.555 0.00 . 173 275 98 SER H H 9.060 0.00 . 174 275 98 SER N N 116.936 0.00 . 175 276 99 PHE H H 8.925 0.00 . 176 276 99 PHE N N 122.402 0.00 . 177 277 100 LYS H H 8.702 0.00 . 178 277 100 LYS N N 119.284 0.00 . 179 278 101 GLU H H 7.803 0.00 . 180 278 101 GLU N N 119.046 0.00 . 181 279 102 SER H H 8.247 0.00 . 182 279 102 SER N N 114.662 0.00 . 183 280 103 LEU H H 7.108 0.00 . 184 280 103 LEU N N 120.352 0.00 . 185 281 104 LEU H H 8.282 0.00 . 186 281 104 LEU N N 116.675 0.00 . 187 282 105 GLY H H 7.844 0.00 . 188 282 105 GLY N N 109.066 0.00 . 189 283 106 PHE H H 7.598 0.00 . 190 283 106 PHE N N 110.369 0.00 . 191 284 107 SER H H 8.641 0.00 . 192 284 107 SER N N 114.928 0.00 . 193 285 108 LYS H H 9.150 0.00 . 194 285 108 LYS N N 125.882 0.00 . 195 286 109 THR H H 8.432 0.00 . 196 286 109 THR N N 114.139 0.00 . 197 287 110 ILE H H 8.290 0.00 . 198 287 110 ILE N N 117.905 0.00 . 199 288 111 GLN H H 7.821 0.00 . 200 288 111 GLN N N 121.444 0.00 . 201 289 112 THR H H 8.764 0.00 . 202 289 112 THR N N 113.691 0.00 . 203 290 113 ILE H H 8.297 0.00 . 204 290 113 ILE N N 120.693 0.00 . 205 291 114 ASP H H 7.865 0.00 . 206 291 114 ASP N N 115.771 0.00 . 207 292 115 GLY H H 7.815 0.00 . 208 292 115 GLY N N 107.851 0.00 . 209 293 116 ARG H H 7.578 0.00 . 210 293 116 ARG N N 119.985 0.00 . 211 294 117 THR H H 8.284 0.00 . 212 294 117 THR N N 115.121 0.00 . 213 295 118 LEU H H 9.141 0.00 . 214 295 118 LEU N N 129.634 0.00 . 215 297 120 LEU H H 8.761 0.00 . 216 297 120 LEU N N 125.519 0.00 . 217 298 121 SER H H 8.234 0.00 . 218 298 121 SER N N 116.642 0.00 . 219 299 122 ARG H H 7.697 0.00 . 220 299 122 ARG N N 122.148 0.00 . 221 301 124 GLN H H 7.210 0.00 . 222 301 124 GLN N N 119.740 0.00 . 223 303 126 VAL H H 8.562 0.00 . 224 303 126 VAL N N 120.472 0.00 . 225 304 127 GLN H H 8.192 0.00 . 226 304 127 GLN N N 123.411 0.00 . 227 307 130 GLN H H 8.014 0.00 . 228 307 130 GLN N N 122.005 0.00 . 229 308 131 THR H H 8.170 0.00 . 230 308 131 THR N N 112.201 0.00 . 231 309 132 SER H H 9.030 0.00 . 232 309 132 SER N N 117.251 0.00 . 233 310 133 THR H H 8.552 0.00 . 234 310 133 THR N N 120.137 0.00 . 235 311 134 TYR H H 9.777 0.00 . 236 311 134 TYR N N 127.804 0.00 . 237 313 136 GLY H H 8.527 0.00 . 238 313 136 GLY N N 111.154 0.00 . 239 314 137 GLN H H 6.657 0.00 . 240 314 137 GLN N N 112.883 0.00 . 241 315 138 GLY H H 8.052 0.00 . 242 315 138 GLY N N 108.245 0.00 . 243 316 139 MET H H 8.678 0.00 . 244 316 139 MET N N 120.573 0.00 . 245 318 141 THR H H 8.567 0.00 . 246 318 141 THR N N 117.333 0.00 . 247 320 143 LYS H H 8.054 0.00 . 248 320 143 LYS N N 114.679 0.00 . 249 321 144 ASN H H 7.122 0.00 . 250 321 144 ASN N N 111.997 0.00 . 251 323 146 SER H H 7.570 0.00 . 252 323 146 SER N N 112.568 0.00 . 253 324 147 GLN H H 8.392 0.00 . 254 324 147 GLN N N 123.429 0.00 . 255 325 148 ARG H H 8.452 0.00 . 256 325 148 ARG N N 121.215 0.00 . 257 326 149 GLY H H 8.578 0.00 . 258 326 149 GLY N N 105.139 0.00 . 259 327 150 ASN H H 9.547 0.00 . 260 327 150 ASN N N 117.794 0.00 . 261 328 151 LEU H H 8.039 0.00 . 262 328 151 LEU N N 120.225 0.00 . 263 329 152 ILE H H 9.668 0.00 . 264 329 152 ILE N N 130.479 0.00 . 265 330 153 VAL H H 9.321 0.00 . 266 330 153 VAL N N 127.764 0.00 . 267 331 154 LYS H H 8.789 0.00 . 268 331 154 LYS N N 126.016 0.00 . 269 332 155 TYR H H 8.419 0.00 . 270 332 155 TYR N N 120.563 0.00 . 271 333 156 LYS H H 8.280 0.00 . 272 333 156 LYS N N 125.902 0.00 . 273 334 157 VAL H H 8.642 0.00 . 274 334 157 VAL N N 127.995 0.00 . 275 335 158 ASP H H 8.499 0.00 . 276 335 158 ASP N N 131.998 0.00 . stop_ save_