data_27887 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone (1H and 15N) Chemical Shift Assignments and Relaxation Parameters for the low complexity domain of FUS in the condensed phase ; _BMRB_accession_number 27887 _BMRB_flat_file_name bmr27887.str _Entry_type original _Submission_date 2019-05-01 _Accession_date 2019-05-01 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Murthy Anastasia C. . 2 Burke Kathleen A. . 3 Fawzi Nicolas L. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 heteronucl_NOE 1 T1_relaxation 1 T2_relaxation 1 stop_ loop_ _Data_type _Data_type_count "15N chemical shifts" 145 "T1 relaxation values" 35 "T2 relaxation values" 43 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2019-12-23 update BMRB 'update entry citation' 2019-06-11 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 26672 'Assignments for the low complexity prion-like domain of Fused in Sarcoma (FUS 1-163)' 27125 'FUS low complexity domain with 12 phosphomimetic S/T to E mutations' stop_ _Original_release_date 2019-05-02 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Molecular interactions underlying liquid-liquid phase separation of the FUS low complexity domain ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 31270472 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Murthy Anastasia . . 2 Dignon Gregory . . 3 Kan Yelena . . 4 Zerze Gul . . 5 Parekh Sapun . . 6 Mittal Jeetain . . 7 Fawzi Nicolas . . stop_ _Journal_abbreviation 'Nat. Struct. Mol. Biol.' _Journal_name_full 'Nature structural & molecular biology' _Journal_volume 26 _Journal_issue 7 _Journal_ISSN 1545-9985 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 637 _Page_last 648 _Year 2019 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Condensed FUS LC' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'FUS 1-163' $FUS_1-163 stop_ _System_molecular_weight . _System_physical_state 'intrinsically disordered' _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details 'The liquid-liquid phase separated state of FUS 1-163 containing 477 mg/mL protein and 65% water content by volume.' save_ ######################## # Monomeric polymers # ######################## save_FUS_1-163 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common FUS_1-163 _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 163 _Mol_residue_sequence ; MASNDYTQQATQSYGAYPTQ PGQGYSQQSSQPYGQQSYSG YSQSTDTSGYGQSSYSSYGQ SQNTGYGTQSTPQGYGSTGG YGSSQSSQSSYGQQSSYPGY GQQPAPSSTSGSYGSSSQSS SYGQPQSGSYSQQPSYGGQQ QSYGQQQSYNPPQGYGQQNQ YNS ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 1 MET 2 2 ALA 3 3 SER 4 4 ASN 5 5 ASP 6 6 TYR 7 7 THR 8 8 GLN 9 9 GLN 10 10 ALA 11 11 THR 12 12 GLN 13 13 SER 14 14 TYR 15 15 GLY 16 16 ALA 17 17 TYR 18 18 PRO 19 19 THR 20 20 GLN 21 21 PRO 22 22 GLY 23 23 GLN 24 24 GLY 25 25 TYR 26 26 SER 27 27 GLN 28 28 GLN 29 29 SER 30 30 SER 31 31 GLN 32 32 PRO 33 33 TYR 34 34 GLY 35 35 GLN 36 36 GLN 37 37 SER 38 38 TYR 39 39 SER 40 40 GLY 41 41 TYR 42 42 SER 43 43 GLN 44 44 SER 45 45 THR 46 46 ASP 47 47 THR 48 48 SER 49 49 GLY 50 50 TYR 51 51 GLY 52 52 GLN 53 53 SER 54 54 SER 55 55 TYR 56 56 SER 57 57 SER 58 58 TYR 59 59 GLY 60 60 GLN 61 61 SER 62 62 GLN 63 63 ASN 64 64 THR 65 65 GLY 66 66 TYR 67 67 GLY 68 68 THR 69 69 GLN 70 70 SER 71 71 THR 72 72 PRO 73 73 GLN 74 74 GLY 75 75 TYR 76 76 GLY 77 77 SER 78 78 THR 79 79 GLY 80 80 GLY 81 81 TYR 82 82 GLY 83 83 SER 84 84 SER 85 85 GLN 86 86 SER 87 87 SER 88 88 GLN 89 89 SER 90 90 SER 91 91 TYR 92 92 GLY 93 93 GLN 94 94 GLN 95 95 SER 96 96 SER 97 97 TYR 98 98 PRO 99 99 GLY 100 100 TYR 101 101 GLY 102 102 GLN 103 103 GLN 104 104 PRO 105 105 ALA 106 106 PRO 107 107 SER 108 108 SER 109 109 THR 110 110 SER 111 111 GLY 112 112 SER 113 113 TYR 114 114 GLY 115 115 SER 116 116 SER 117 117 SER 118 118 GLN 119 119 SER 120 120 SER 121 121 SER 122 122 TYR 123 123 GLY 124 124 GLN 125 125 PRO 126 126 GLN 127 127 SER 128 128 GLY 129 129 SER 130 130 TYR 131 131 SER 132 132 GLN 133 133 GLN 134 134 PRO 135 135 SER 136 136 TYR 137 137 GLY 138 138 GLY 139 139 GLN 140 140 GLN 141 141 GLN 142 142 SER 143 143 TYR 144 144 GLY 145 145 GLN 146 146 GLN 147 147 GLN 148 148 SER 149 149 TYR 150 150 ASN 151 151 PRO 152 152 PRO 153 153 GLN 154 154 GLY 155 155 TYR 156 156 GLY 157 157 GLN 158 158 GLN 159 159 ASN 160 160 GLN 161 161 TYR 162 162 ASN 163 163 SER stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $FUS_1-163 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $FUS_1-163 'recombinant technology' . Escherichia coli 'BL21 Star' Unknown 'Addgene: FUS_anion; Plasmid #98656.' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $FUS_1-163 27.8 mM '[U-99% 15N]' MES 50 mM 'natural abundance' 'sodium chloride' 150 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task 'peak picking' processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 850 _Details 'TCI cryoprobe' save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_hsqct2etf3gpsitc3d_2 _Saveframe_category NMR_applied_experiment _Experiment_name hsqct2etf3gpsitc3d _Sample_label $sample_1 save_ save_hsqct1etf3gpsitc3d_3 _Saveframe_category NMR_applied_experiment _Experiment_name hsqct1etf3gpsitc3d _Sample_label $sample_1 save_ save_hsqcnoef3gpsi_4 _Saveframe_category NMR_applied_experiment _Experiment_name hsqcnoef3gpsi _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.15 . M pH 5.5 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0 external direct . . . 1 na N 15 nitrogen ppm 0 external indirect . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details 'Assignments were transferred from the dispersed phase using a HSQC-NOESY and 2D HSQC.' loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'FUS 1-163' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 MET N N 120.068 . . 2 2 2 ALA N N 125.869 . . 3 3 3 SER N N 115.579 . . 4 4 4 ASN N N 120.093 . . 5 5 5 ASP N N 120.191 . . 6 7 7 THR N N 114.982 . . 7 8 8 GLN N N 122.072 . . 8 9 9 GLN N N 121.111 . . 9 10 10 ALA N N 124.964 . . 10 11 11 THR N N 112.949 . . 11 12 12 GLN N N 122.234 . . 12 13 13 SER N N 116.770 . . 13 14 14 TYR N N 121.983 . . 14 15 15 GLY N N 110.212 . . 15 16 16 ALA N N 123.211 . . 16 17 17 TYR N N 119.991 . . 17 19 19 THR N N 114.121 . . 18 20 20 GLN N N 123.065 . . 19 22 22 GLY N N 109.432 . . 20 23 23 GLN N N 119.404 . . 21 24 24 GLY N N 109.687 . . 22 25 25 TYR N N 120.023 . . 23 26 26 SER N N 117.616 . . 24 27 27 GLN N N 122.277 . . 25 28 28 GLN N N 121.051 . . 26 29 29 SER N N 116.663 . . 27 30 30 SER N N 117.463 . . 28 31 31 GLN N N 122.299 . . 29 33 33 TYR N N 120.020 . . 30 34 34 GLY N N 110.386 . . 31 35 35 GLN N N 119.595 . . 32 36 36 GLN N N 121.420 . . 33 37 37 SER N N 116.620 . . 34 38 38 TYR N N 122.035 . . 35 39 39 SER N N 117.728 . . 36 40 40 GLY N N 110.023 . . 37 41 41 TYR N N 119.989 . . 38 42 42 SER N N 117.731 . . 39 43 43 GLN N N 122.287 . . 40 44 44 SER N N 116.764 . . 41 45 45 THR N N 115.346 . . 42 46 46 ASP N N 122.737 . . 43 47 47 THR N N 114.508 . . 44 48 48 SER N N 118.225 . . 45 49 49 GLY N N 110.205 . . 46 50 50 TYR N N 120.146 . . 47 51 51 GLY N N 110.641 . . 48 52 52 GLN N N 119.711 . . 49 53 53 SER N N 116.835 . . 50 54 54 SER N N 117.512 . . 51 55 55 TYR N N 121.840 . . 52 56 56 SER N N 116.897 . . 53 57 57 SER N N 117.688 . . 54 58 58 TYR N N 121.822 . . 55 59 59 GLY N N 110.362 . . 56 60 60 GLN N N 119.630 . . 57 61 61 SER N N 116.788 . . 58 62 62 GLN N N 121.443 . . 59 63 63 ASN N N 119.684 . . 60 64 64 THR N N 113.675 . . 61 65 65 GLY N N 110.423 . . 62 67 67 GLY N N 110.654 . . 63 68 68 THR N N 113.400 . . 64 69 69 GLN N N 122.309 . . 65 70 70 SER N N 117.131 . . 66 71 71 THR N N 117.469 . . 67 73 73 GLN N N 120.336 . . 68 74 74 GLY N N 109.764 . . 69 75 75 TYR N N 120.199 . . 70 76 76 GLY N N 110.758 . . 71 77 77 SER N N 115.620 . . 72 78 78 THR N N 114.817 . . 73 79 79 GLY N N 110.638 . . 74 80 80 GLY N N 108.341 . . 75 81 81 TYR N N 120.250 . . 76 82 82 GLY N N 110.804 . . 77 83 83 SER N N 115.534 . . 78 84 84 SER N N 117.704 . . 79 85 85 GLN N N 121.893 . . 80 86 86 SER N N 116.499 . . 81 87 87 SER N N 117.659 . . 82 88 88 GLN N N 121.849 . . 83 89 89 SER N N 116.415 . . 84 90 90 SER N N 117.486 . . 85 92 92 GLY N N 110.271 . . 86 93 93 GLN N N 119.679 . . 87 95 95 SER N N 116.384 . . 88 96 96 SER N N 117.210 . . 89 97 97 TYR N N 122.676 . . 90 99 99 GLY N N 108.460 . . 91 100 100 TYR N N 119.977 . . 92 101 101 GLY N N 111.084 . . 93 102 102 GLN N N 119.546 . . 94 105 105 ALA N N 125.395 . . 95 107 107 SER N N 115.636 . . 96 108 108 SER N N 117.544 . . 97 109 109 THR N N 114.837 . . 98 110 110 SER N N 117.721 . . 99 111 111 GLY N N 110.738 . . 100 112 112 SER N N 115.478 . . 101 113 113 TYR N N 122.238 . . 102 114 114 GLY N N 110.512 . . 103 115 115 SER N N 115.634 . . 104 116 116 SER N N 117.744 . . 105 117 117 SER N N 117.684 . . 106 118 118 GLN N N 121.838 . . 107 119 119 SER N N 116.552 . . 108 120 120 SER N N 117.630 . . 109 121 121 SER N N 117.480 . . 110 122 122 TYR N N 121.905 . . 111 123 123 GLY N N 110.456 . . 112 124 124 GLN N N 120.436 . . 113 126 126 GLN N N 120.645 . . 114 127 127 SER N N 116.821 . . 115 128 128 GLY N N 110.731 . . 116 129 129 SER N N 115.415 . . 117 131 131 SER N N 116.940 . . 118 132 132 GLN N N 121.920 . . 119 133 133 GLN N N 122.461 . . 120 135 135 SER N N 115.599 . . 121 136 136 TYR N N 122.052 . . 122 137 137 GLY N N 110.805 . . 123 138 138 GLY N N 108.266 . . 124 139 139 GLN N N 119.666 . . 125 140 140 GLN N N 121.518 . . 126 141 141 GLN N N 121.473 . . 127 142 142 SER N N 116.688 . . 128 144 144 GLY N N 110.263 . . 129 145 145 GLN N N 119.750 . . 130 146 146 GLN N N 121.457 . . 131 147 147 GLN N N 121.436 . . 132 148 148 SER N N 116.752 . . 133 149 149 TYR N N 122.372 . . 134 150 150 ASN N N 122.870 . . 135 153 153 GLN N N 119.956 . . 136 154 154 GLY N N 109.875 . . 137 155 155 TYR N N 120.027 . . 138 156 156 GLY N N 110.686 . . 139 157 157 GLN N N 119.717 . . 140 158 158 GLN N N 121.028 . . 141 159 159 ASN N N 119.613 . . 142 160 160 GLN N N 120.139 . . 143 161 161 TYR N N 120.345 . . 144 162 162 ASN N N 120.350 . . 145 163 163 SER N N 121.306 . . stop_ save_ save_heteronuclear_T1_list_1 _Saveframe_category T1_relaxation _Details 'R1 relaxation rate constant values (in units of radians/sec) for FUS 1-163 in the condensed phase.' loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 850 _T1_coherence_type Sz _T1_value_units s-1 _Mol_system_component_name 'FUS 1-163' _Text_data_format . _Text_data . loop_ _T1_ID _Residue_seq_code _Residue_label _Atom_name _T1_value _T1_value_error 1 3 SER N 1.01398282313098 0.00727287815663313 2 4 ASN N 1.17720460993325 0.00337439323306032 3 7 THR N 0.852660300136426 0.0220811044358478 4 9 GLN N 0.866851595006935 0.0224293679776344 5 10 ALA N 0.893655049151028 0.0243783397233256 6 11 THR N 0.812215724496426 0.0202192727357274 7 24 GLY N 0.848176420695505 0.0243599727974679 8 33 TYR N 0.965064659332175 0.00532930023713674 9 34 GLY N 0.901957247226481 0.009008595198291 10 51 GLY N 0.972006220839813 0.00329648142163064 11 64 THR N 0.81406707912732 0.0229337126754289 12 68 THR N 0.802761499558481 0.0199986798361482 13 69 GLN N 0.959969280983008 0.0193283667440415 14 78 THR N 0.950570342205323 0.010704693001375 15 80 GLY N 0.990883868410622 0.00941291964639424 16 85 GLN N 0.966090232827746 0.00935222299300187 17 95 SER N 0.941885655081473 0.00663023056772805 18 110 SER N 0.966183574879227 0.00600984611289823 19 113 TYR N 0.89437438511761 0.00792442863916232 20 115 SER N 0.930578820026056 0.0056382992095263 21 116 SER N 1.01647709368869 0.0046851666458001 22 117 SER N 0.994925878022087 0.00486736473531035 23 118 GLN N 0.987751876728566 0.00733022849101805 24 127 SER N 0.873515024458421 0.00717328629773384 25 129 SER N 0.949307005885703 0.00477093312276533 26 138 GLY N 0.922168941350055 0.0133232585159822 27 139 GLN N 0.832431532506451 0.0140619528796853 28 143 TYR N 0.809716599190283 0.00869638345854296 29 146 GLN N 0.859328005499699 0.00933229446607564 30 157 GLN N 0.771664480283972 0.00677401879318862 31 158 GLN N 0.846883468834688 0.0129484512215791 32 159 ASN N 0.876193814071673 0.0101267148844191 33 161 TYR N 0.901875901875902 0.0104080008746341 34 162 ASN N 1.01149053244862 0.00694902871392422 35 163 SER N 1.0801118995928 0.00192265864332986 stop_ save_ save_heteronuclear_T2_list_1 _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 850 _T2_coherence_type S(+,-) _T2_value_units s-1 _Mol_system_component_name 'FUS 1-163' _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 3 SER N 9.48226815854352 0.0440708350864032 . . 2 4 ASN N 14.6337894197702 0.0424813985199073 . . 3 6 TYR N 31.5457413249211 1.05048272848452 . . 4 7 THR N 37.5855070284898 2.14559288728025 . . 5 9 GLN N 16.1381424997983 1.06340632765815 . . 6 10 ALA N 28.2039711191336 1.88270860515209 . . 7 11 THR N 35.7922617130176 1.70741016485342 . . 8 14 TYR N 37.9968082681055 0.820520874509 . . 9 24 GLY N 42.6511984986778 1.85125736934927 . . 10 33 TYR N 24.3409682837183 0.229672061567967 . . 11 34 GLY N 40.3909847322078 1.35367487348887 . . 12 43 GLN N 36.4604222116892 2.32873544296316 . . 13 47 THR N 31.2139089178138 2.54546774905545 . . 14 51 GLY N 27.332112498975 0.173391352345604 . . 15 55 TYR N 36.6824401159165 1.37213394874962 . . 16 58 TYR N 38.3288616328095 1.35963132367365 . . 17 63 ASN N 32.5785958625183 0.755181921185454 . . 18 64 THR N 34.0831629175187 2.0007249048908 . . 19 68 THR N 37.684654808562 1.92683569260262 . . 20 69 GLN N 26.459226332222 1.32526603121023 . . 21 78 THR N 28.4131268646115 0.654798481979956 . . 22 80 GLY N 26.7909767990141 0.373605800875509 . . 23 85 GLN N 27.5345558676139 0.503139131050151 . . 24 95 SER N 29.8694704142896 0.376870912647686 . . 25 110 SER N 28.0685996575631 0.327116223990433 . . 26 113 TYR N 31.6696224980998 0.533582300435317 . . 27 115 SER N 28.2382176037049 0.30181692973693 . . 28 116 SER N 27.2294077603812 0.219132402734484 . . 29 117 SER N 27.4137836504194 0.239018822847221 . . 30 118 GLN N 25.142052597174 0.321083656577322 . . 31 127 SER N 37.0123621289511 0.597373677698783 . . 32 129 SER N 26.3407438626067 0.225184802561664 . . 33 132 GLN N 31.2822598304502 0.941823869673815 . . 34 138 GLY N 32.517152798101 0.825861123607602 . . 35 139 GLN N 33.357795716859 1.28179664441806 . . 36 146 GLN N 26.195840100592 0.62282762308773 . . 37 153 GLN N 29.1154719618005 5.16009675557344 . . 38 154 GLY N 22.0128555076165 1.4217700890877 . . 39 157 GLN N 34.6909040449594 0.761866837254431 . . 40 158 GLN N 31.8532203605785 0.888245494492757 . . 41 161 TYR N 31.252929962184 0.637275885905776 . . 42 162 ASN N 23.1336895921531 0.234317969734196 . . 43 163 SER N 11.7065861253541 0.0157208621724028 . . stop_ save_ save_heteronuclear_noe_list_1 _Saveframe_category heteronuclear_NOE _Details . loop_ _Experiment_label hsqcnoef3gpsi stop_ loop_ _Sample_label . stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 850 _Mol_system_component_name 'FUS 1-163' _Atom_one_atom_name N _Atom_two_atom_name H _Heteronuclear_NOE_value_type 'peak height' _NOE_reference_value 0 _NOE_reference_description . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Heteronuclear_NOE_value _Heteronuclear_NOE_value_error 3 SER 0.1223 0.00290232625978488 4 ASN 0.4202 0.00142508072932514 7 THR 0.5667 0.00808632034051944 9 GLN 0.5212 0.00801100699735742 10 ALA 0.4993 0.00838037341763065 11 THR 0.4630 0.00715632869113188 24 GLY 0.5034 0.00944323380339281 33 TYR 0.5199 0.00199077236739129 34 GLY 0.1628 0.00506769150446518 51 GLY 0.5212 0.00133369343323493 64 THR 0.5051 0.00830845575609309 68 THR 0.5265 0.00718348914827774 69 GLN 0.4927 0.00665023862411727 78 THR 0.4821 0.00349688295741624 80 GLY 0.5299 0.00330384952838454 85 GLN 0.5097 0.00426692590337296 95 SER 0.5086 0.0022064685632528 110 SER 0.5117 0.00195092552012132 113 TYR 0.5311 0.00253022348906517 115 SER 0.5033 0.00198662630221699 116 SER 0.4848 0.00204949513072231 117 SER 0.5071 0.00215416275083646 118 GLN 0.5149 0.00316764964307503 127 SER 0.4925 0.00275195358050828 129 SER 0.5067 0.00197334944046819 138 GLY 0.5429 0.00510864200184863 139 GLN 0.5258 0.00455137500232377 143 TYR 0.5426 0.00251335194993749 146 GLN 0.5112 0.00298446966405837 157 GLN 0.5236 0.00225063945498714 158 GLN 0.5340 0.00495378298677844 159 ASN 0.5294 0.00386332642790142 161 TYR 0.5416 0.00318747187762766 162 ASN 0.5291 0.00232081810388109 163 SER 0.3096 0.000957435446378144 stop_ save_