data_27664 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; UbcH7-Ub isopeptide conjugate ; _BMRB_accession_number 27664 _BMRB_flat_file_name bmr27664.str _Entry_type original _Submission_date 2018-10-28 _Accession_date 2018-10-28 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'HN backbone shifts for UbcH7 (C17S, C86K,C137S) covalently attached via K86 side chain to the C-terminal Gly CO in Ubiquitin.' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Condos Tara EC . 2 Shaw Gary S. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 196 "15N chemical shifts" 208 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2018-11-02 original BMRB . stop_ loop_ _Related_BMRB_accession_number _Relationship 15498 UbcH7 4769 'Yeast ubiquitin' stop_ _Original_release_date 2018-10-29 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Synergistic Recruitment of UbcH7~Ub and Phosphorylated Ubl Domain Triggers Parkin Activation ; _Citation_status submitted _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Condos Tara EC . 2 Shaw Gary S. . stop_ _Journal_abbreviation 'EMBO J.' _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . loop_ _Keyword covalent 'ubiquitin conjugating enzyme' stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name UbcH7-Ub _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label UbcH7 $UbcH7 Ub $Ub stop_ _System_molecular_weight 25000 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . loop_ _Biological_function 'Accepts and transfers ubiquitin' stop_ _Database_query_date . _Details 'E2 conjugating enzyme UbcH7 linked via isopeptide to ubiquitin' save_ ######################## # Monomeric polymers # ######################## save_UbcH7 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common UbcH7 _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function 'ubiquitin conjugating enzyme' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 154 _Mol_residue_sequence ; MAASRRLMKELEEIRKSGMK NFRNIQVDEANLLTWQGLIV PDNPPYDKGAFRIEINFPAE YPFKPPKITFKTKIYHPNID EKGQVKLPVISAENWKPATK TDQVIQSLIALVNDPQPEHP LRADLAEEYSKDRKKFSKNA EEFTKKYGEKRPVD ; loop_ _Residue_seq_code _Residue_label 1 MET 2 ALA 3 ALA 4 SER 5 ARG 6 ARG 7 LEU 8 MET 9 LYS 10 GLU 11 LEU 12 GLU 13 GLU 14 ILE 15 ARG 16 LYS 17 SER 18 GLY 19 MET 20 LYS 21 ASN 22 PHE 23 ARG 24 ASN 25 ILE 26 GLN 27 VAL 28 ASP 29 GLU 30 ALA 31 ASN 32 LEU 33 LEU 34 THR 35 TRP 36 GLN 37 GLY 38 LEU 39 ILE 40 VAL 41 PRO 42 ASP 43 ASN 44 PRO 45 PRO 46 TYR 47 ASP 48 LYS 49 GLY 50 ALA 51 PHE 52 ARG 53 ILE 54 GLU 55 ILE 56 ASN 57 PHE 58 PRO 59 ALA 60 GLU 61 TYR 62 PRO 63 PHE 64 LYS 65 PRO 66 PRO 67 LYS 68 ILE 69 THR 70 PHE 71 LYS 72 THR 73 LYS 74 ILE 75 TYR 76 HIS 77 PRO 78 ASN 79 ILE 80 ASP 81 GLU 82 LYS 83 GLY 84 GLN 85 VAL 86 LYS 87 LEU 88 PRO 89 VAL 90 ILE 91 SER 92 ALA 93 GLU 94 ASN 95 TRP 96 LYS 97 PRO 98 ALA 99 THR 100 LYS 101 THR 102 ASP 103 GLN 104 VAL 105 ILE 106 GLN 107 SER 108 LEU 109 ILE 110 ALA 111 LEU 112 VAL 113 ASN 114 ASP 115 PRO 116 GLN 117 PRO 118 GLU 119 HIS 120 PRO 121 LEU 122 ARG 123 ALA 124 ASP 125 LEU 126 ALA 127 GLU 128 GLU 129 TYR 130 SER 131 LYS 132 ASP 133 ARG 134 LYS 135 LYS 136 PHE 137 SER 138 LYS 139 ASN 140 ALA 141 GLU 142 GLU 143 PHE 144 THR 145 LYS 146 LYS 147 TYR 148 GLY 149 GLU 150 LYS 151 ARG 152 PRO 153 VAL 154 ASP stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 15498 . . . . . . BMRB 4769 . . . . . . stop_ save_ save_Ub _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Ub _Molecular_mass . _Mol_thiol_state 'all free' loop_ _Biological_function 'ubiquitin conjugating enzyme' stop_ _Details . _Residue_count 76 _Mol_residue_sequence ; MQIFVKTLTGKTITLEVESS DTIDNVKSKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 201 MET 2 202 GLN 3 203 ILE 4 204 PHE 5 205 VAL 6 206 LYS 7 207 THR 8 208 LEU 9 209 THR 10 210 GLY 11 211 LYS 12 212 THR 13 213 ILE 14 214 THR 15 215 LEU 16 216 GLU 17 217 VAL 18 218 GLU 19 219 SER 20 220 SER 21 221 ASP 22 222 THR 23 223 ILE 24 224 ASP 25 225 ASN 26 226 VAL 27 227 LYS 28 228 SER 29 229 LYS 30 230 ILE 31 231 GLN 32 232 ASP 33 233 LYS 34 234 GLU 35 235 GLY 36 236 ILE 37 237 PRO 38 238 PRO 39 239 ASP 40 240 GLN 41 241 GLN 42 242 ARG 43 243 LEU 44 244 ILE 45 245 PHE 46 246 ALA 47 247 GLY 48 248 LYS 49 249 GLN 50 250 LEU 51 251 GLU 52 252 ASP 53 253 GLY 54 254 ARG 55 255 THR 56 256 LEU 57 257 SER 58 258 ASP 59 259 TYR 60 260 ASN 61 261 ILE 62 262 GLN 63 263 LYS 64 264 GLU 65 265 SER 66 266 THR 67 267 LEU 68 268 HIS 69 269 LEU 70 270 VAL 71 271 LEU 72 272 ARG 73 273 LEU 74 274 ARG 75 275 GLY 76 276 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 15498 . . . . . . BMRB 4769 . . . . . . stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $UbcH7 Human 9606 Eukaryota Metazoa Homo sapiens $Ub Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Variant _Vector_name $UbcH7 'recombinant technology' . Escherichia coli BL21(DE3) CodonPlus-RIL pET28a $Ub 'recombinant technology' . Escherichia coli BL21(DE3) CodonPlus-RIL pET28a stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $UbcH7 0.11 mM '[U-99% 2H]' $Ub 0.11 mM '[U-99% 2H]' DSS 0.01 mM 'natural abundance' HEPES 25 mM 'natural abundance' 'sodium chloride' 50 mM 'natural abundance' TCEP 0.5 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version 9.2 loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_NMRViewJ _Saveframe_category software _Name NMRViewJ _Version 9.2.0 loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ save_VNMRJ _Saveframe_category software _Name VNMRJ _Version 4.2 loop_ _Vendor _Address _Electronic_address Varian . . stop_ loop_ _Task collection stop_ _Details . save_ save_NMRView _Saveframe_category software _Name NMRView _Version 9.2.0 loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 50 . mM pH 7 0.05 pH pressure 1 . atm temperature 298 0.1 K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details 'Assignments were made by comparing to BMRB 15498, 4769 and published literature.' loop_ _Software_label $NMRView stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name UbcH7 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 6 6 ARG H H 7.792 0.01 . 2 6 6 ARG N N 120.084 0.2 . 3 7 7 LEU H H 8.188 0.01 . 4 7 7 LEU N N 117.758 0.2 . 5 8 8 MET H H 8.361 0.01 . 6 8 8 MET N N 117.861 0.2 . 7 9 9 LYS H H 7.732 0.01 . 8 9 9 LYS N N 120.017 0.2 . 9 10 10 GLU H H 8.606 0.01 . 10 10 10 GLU N N 119.762 0.2 . 11 11 11 LEU H H 8.808 0.01 . 12 11 11 LEU N N 120.762 0.2 . 13 12 12 GLU H H 7.763 0.01 . 14 12 12 GLU N N 118.602 0.2 . 15 13 13 GLU H H 8.299 0.01 . 16 13 13 GLU N N 118.481 0.2 . 17 14 14 ILE H H 8.559 0.01 . 18 14 14 ILE N N 121.779 0.2 . 19 15 15 ARG H H 8.556 0.01 . 20 15 15 ARG N N 121.201 0.2 . 21 16 16 LYS H H 7.748 0.01 . 22 16 16 LYS N N 117.443 0.2 . 23 17 17 SER H H 7.786 0.01 . 24 17 17 SER N N 114.220 0.2 . 25 18 18 GLY H H 8.112 0.01 . 26 18 18 GLY N N 110.559 0.2 . 27 19 19 MET H H 8.392 0.01 . 28 19 19 MET N N 119.321 0.2 . 29 20 20 LYS H H 8.726 0.01 . 30 20 20 LYS N N 122.538 0.2 . 31 21 21 ASN H H 8.202 0.01 . 32 21 21 ASN N N 111.901 0.2 . 33 22 22 PHE H H 7.093 0.01 . 34 22 22 PHE N N 120.390 0.2 . 35 23 23 ARG H H 8.924 0.01 . 36 23 23 ARG N N 122.321 0.2 . 37 24 24 ASN H H 8.708 0.01 . 38 24 24 ASN N N 116.926 0.2 . 39 25 25 ILE H H 7.953 0.01 . 40 25 25 ILE N N 118.159 0.2 . 41 26 26 GLN H H 9.212 0.01 . 42 26 26 GLN N N 130.049 0.2 . 43 27 27 VAL H H 8.318 0.01 . 44 27 27 VAL N N 123.003 0.2 . 45 28 28 ASP H H 8.893 0.01 . 46 28 28 ASP N N 127.249 0.2 . 47 29 29 GLU H H 8.809 0.01 . 48 29 29 GLU N N 125.575 0.2 . 49 30 30 ALA H H 8.473 0.01 . 50 30 30 ALA N N 119.973 0.2 . 51 31 31 ASN H H 8.019 0.01 . 52 31 31 ASN N N 115.248 0.2 . 53 32 32 LEU H H 8.662 0.01 . 54 32 32 LEU N N 121.738 0.2 . 55 33 33 LEU H H 7.558 0.01 . 56 33 33 LEU N N 112.507 0.2 . 57 34 34 THR H H 7.405 0.01 . 58 34 34 THR N N 114.037 0.2 . 59 35 35 TRP H H 9.637 0.01 . 60 35 35 TRP N N 127.297 0.2 . 61 36 36 GLN H H 8.798 0.01 . 62 36 36 GLN N N 118.621 0.2 . 63 37 37 GLY H H 8.167 0.01 . 64 37 37 GLY N N 103.744 0.2 . 65 38 38 LEU H H 8.629 0.01 . 66 38 38 LEU N N 120.695 0.2 . 67 39 39 ILE H H 9.686 0.01 . 68 39 39 ILE N N 124.519 0.2 . 69 40 40 VAL H H 8.653 0.01 . 70 40 40 VAL N N 129.175 0.2 . 71 42 42 ASP N N 119.047 0.2 . 72 43 43 ASN H H 7.853 0.01 . 73 43 43 ASN N N 116.135 0.2 . 74 46 46 TYR N N 121.252 0.2 . 75 47 47 ASP H H 8.123 0.01 . 76 47 47 ASP N N 115.994 0.2 . 77 48 48 LYS H H 8.099 0.01 . 78 48 48 LYS N N 115.496 0.2 . 79 49 49 GLY H H 7.742 0.01 . 80 49 49 GLY N N 107.145 0.2 . 81 50 50 ALA H H 7.638 0.01 . 82 50 50 ALA N N 118.434 0.2 . 83 51 51 PHE H H 8.853 0.01 . 84 51 51 PHE N N 115.226 0.2 . 85 52 52 ARG H H 10.045 0.01 . 86 52 52 ARG N N 126.443 0.2 . 87 53 53 ILE H H 8.931 0.01 . 88 53 53 ILE N N 117.657 0.2 . 89 54 54 GLU H H 8.997 0.01 . 90 54 54 GLU N N 121.543 0.2 . 91 55 55 ILE H H 9.410 0.01 . 92 55 55 ILE N N 125.694 0.2 . 93 56 56 ASN H H 8.691 0.01 . 94 56 56 ASN N N 123.469 0.2 . 95 57 57 PHE H H 9.664 0.01 . 96 57 57 PHE N N 127.011 0.2 . 97 59 59 ALA N N 122.401 0.2 . 98 60 60 GLU H H 8.391 0.01 . 99 60 60 GLU N N 110.663 0.2 . 100 61 61 TYR H H 8.112 0.01 . 101 61 61 TYR N N 123.915 0.2 . 102 63 63 PHE N N 125.519 0.2 . 103 64 64 LYS H H 7.011 0.01 . 104 64 64 LYS N N 117.163 0.2 . 105 67 67 LYS N N 116.935 0.2 . 106 68 68 ILE H H 8.504 0.01 . 107 68 68 ILE N N 124.545 0.2 . 108 69 69 THR H H 8.713 0.01 . 109 69 69 THR N N 120.523 0.2 . 110 70 70 PHE H H 10.451 0.01 . 111 70 70 PHE N N 126.908 0.2 . 112 71 71 LYS H H 9.040 0.01 . 113 71 71 LYS N N 123.834 0.2 . 114 72 72 THR H H 7.477 0.01 . 115 72 72 THR N N 118.849 0.2 . 116 73 73 LYS H H 9.022 0.01 . 117 73 73 LYS N N 126.708 0.2 . 118 74 74 ILE H H 8.033 0.01 . 119 74 74 ILE N N 118.898 0.2 . 120 75 75 TYR H H 8.436 0.01 . 121 75 75 TYR N N 131.905 0.2 . 122 76 76 HIS H H 8.780 0.01 . 123 76 76 HIS N N 128.809 0.2 . 124 78 78 ASN N N 118.968 0.2 . 125 79 79 ILE H H 7.268 0.01 . 126 79 79 ILE N N 120.325 0.2 . 127 80 80 ASP H H 8.671 0.01 . 128 80 80 ASP N N 124.895 0.2 . 129 81 81 GLU H H 9.149 0.01 . 130 81 81 GLU N N 115.894 0.2 . 131 82 82 LYS H H 8.112 0.01 . 132 82 82 LYS N N 118.436 0.2 . 133 83 83 GLY H H 8.140 0.01 . 134 83 83 GLY N N 109.547 0.2 . 135 84 84 GLN H H 8.673 0.01 . 136 84 84 GLN N N 120.657 0.2 . 137 85 85 VAL H H 8.186 0.01 . 138 85 85 VAL N N 117.765 0.2 . 139 86 86 LYS H H 8.108 0.01 . 140 86 86 LYS N N 129.717 0.2 . 141 87 87 LEU H H 7.651 0.01 . 142 87 87 LEU N N 125.501 0.2 . 143 89 89 VAL N N 113.872 0.2 . 144 90 90 ILE H H 7.391 0.01 . 145 90 90 ILE N N 107.145 0.2 . 146 91 91 SER H H 7.772 0.01 . 147 91 91 SER N N 118.428 0.2 . 148 92 92 ALA H H 9.062 0.01 . 149 92 92 ALA N N 126.532 0.2 . 150 93 93 GLU H H 9.043 0.01 . 151 93 93 GLU N N 114.369 0.2 . 152 94 94 ASN H H 7.483 0.01 . 153 94 94 ASN N N 114.690 0.2 . 154 95 95 TRP H H 7.931 0.01 . 155 95 95 TRP N N 121.710 0.2 . 156 96 96 LYS H H 6.708 0.01 . 157 96 96 LYS N N 126.486 0.2 . 158 98 98 ALA N N 115.706 0.2 . 159 99 99 THR H H 7.504 0.01 . 160 99 99 THR N N 117.992 0.2 . 161 100 100 LYS H H 8.169 0.01 . 162 100 100 LYS N N 124.696 0.2 . 163 101 101 THR H H 9.512 0.01 . 164 101 101 THR N N 119.993 0.2 . 165 102 102 ASP H H 9.427 0.01 . 166 102 102 ASP N N 120.882 0.2 . 167 103 103 GLN H H 7.436 0.01 . 168 103 103 GLN N N 120.640 0.2 . 169 104 104 VAL H H 7.740 0.01 . 170 104 104 VAL N N 122.025 0.2 . 171 105 105 ILE H H 8.468 0.01 . 172 105 105 ILE N N 118.424 0.2 . 173 106 106 GLN H H 8.310 0.01 . 174 106 106 GLN N N 118.438 0.2 . 175 107 107 SER H H 8.153 0.01 . 176 107 107 SER N N 116.259 0.2 . 177 108 108 LEU H H 8.274 0.01 . 178 108 108 LEU N N 124.581 0.2 . 179 109 109 ILE H H 8.582 0.01 . 180 109 109 ILE N N 119.880 0.2 . 181 110 110 ALA H H 7.890 0.01 . 182 110 110 ALA N N 120.611 0.2 . 183 111 111 LEU H H 7.564 0.01 . 184 111 111 LEU N N 120.311 0.2 . 185 112 112 VAL H H 7.985 0.01 . 186 112 112 VAL N N 119.478 0.2 . 187 113 113 ASN H H 7.930 0.01 . 188 113 113 ASN N N 111.487 0.2 . 189 114 114 ASP H H 8.032 0.01 . 190 114 114 ASP N N 118.403 0.2 . 191 116 116 GLN N N 118.597 0.2 . 192 118 118 GLU N N 117.875 0.2 . 193 119 119 HIS H H 7.014 0.01 . 194 119 119 HIS N N 115.054 0.2 . 195 121 121 LEU N N 115.662 0.2 . 196 122 122 ARG H H 7.636 0.01 . 197 122 122 ARG N N 118.475 0.2 . 198 124 124 ASP H H 9.253 0.01 . 199 124 124 ASP N N 117.256 0.2 . 200 125 125 LEU H H 7.547 0.01 . 201 125 125 LEU N N 123.287 0.2 . 202 126 126 ALA H H 8.021 0.01 . 203 126 126 ALA N N 121.292 0.2 . 204 127 127 GLU H H 7.868 0.01 . 205 127 127 GLU N N 119.094 0.2 . 206 128 128 GLU H H 7.778 0.01 . 207 128 128 GLU N N 121.092 0.2 . 208 129 129 TYR H H 8.512 0.01 . 209 129 129 TYR N N 116.867 0.2 . 210 130 130 SER H H 7.831 0.01 . 211 130 130 SER N N 110.481 0.2 . 212 131 131 LYS H H 8.506 0.01 . 213 131 131 LYS N N 119.285 0.2 . 214 132 132 ASP H H 8.521 0.01 . 215 132 132 ASP N N 122.360 0.2 . 216 133 133 ARG H H 8.505 0.01 . 217 133 133 ARG N N 124.381 0.2 . 218 134 134 LYS H H 8.442 0.01 . 219 134 134 LYS N N 118.386 0.2 . 220 135 135 LYS H H 7.541 0.01 . 221 135 135 LYS N N 123.587 0.2 . 222 136 136 PHE H H 8.511 0.01 . 223 136 136 PHE N N 118.688 0.2 . 224 137 137 SER H H 8.890 0.01 . 225 137 137 SER N N 114.187 0.2 . 226 138 138 LYS H H 7.679 0.01 . 227 138 138 LYS N N 122.425 0.2 . 228 139 139 ASN H H 8.113 0.01 . 229 139 139 ASN N N 118.523 0.2 . 230 140 140 ALA H H 8.960 0.01 . 231 140 140 ALA N N 124.001 0.2 . 232 141 141 GLU H H 8.781 0.01 . 233 141 141 GLU N N 123.179 0.2 . 234 142 142 GLU H H 8.104 0.01 . 235 142 142 GLU N N 120.030 0.2 . 236 143 143 PHE H H 8.363 0.01 . 237 143 143 PHE N N 122.012 0.2 . 238 144 144 THR H H 8.800 0.01 . 239 144 144 THR N N 120.319 0.2 . 240 145 145 LYS H H 8.090 0.01 . 241 145 145 LYS N N 121.488 0.2 . 242 146 146 LYS H H 7.789 0.01 . 243 146 146 LYS N N 117.227 0.2 . 244 147 147 TYR H H 7.898 0.01 . 245 147 147 TYR N N 112.571 0.2 . 246 148 148 GLY H H 8.193 0.01 . 247 148 148 GLY N N 108.335 0.2 . 248 149 149 GLU H H 8.128 0.01 . 249 149 149 GLU N N 117.418 0.2 . 250 150 150 LYS H H 8.319 0.01 . 251 150 150 LYS N N 120.262 0.2 . 252 151 151 ARG H H 8.498 0.01 . 253 151 151 ARG N N 123.874 0.2 . 254 153 153 VAL N N 119.039 0.2 . 255 154 154 ASP H H 7.986 0.01 . 256 154 154 ASP N N 128.448 0.2 . stop_ save_ save_assigned_chem_shift_list_2 _Saveframe_category assigned_chemical_shifts _Details 'Assignments were made by comparing to BMRB 15498, 4769 and published literature.' loop_ _Software_label $NMRView stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name Ub _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 201 1 MET H H 9.138 0.01 . 2 201 1 MET N N 118.706 0.2 . 3 202 2 GLN H H 8.288 0.01 . 4 202 2 GLN N N 120.090 0.2 . 5 203 3 ILE H H 8.354 0.01 . 6 203 3 ILE N N 114.965 0.2 . 7 204 4 PHE H H 8.619 0.01 . 8 204 4 PHE N N 118.572 0.2 . 9 205 5 VAL H H 9.297 0.01 . 10 205 5 VAL N N 121.189 0.2 . 11 206 6 LYS H H 8.996 0.01 . 12 206 6 LYS N N 127.945 0.2 . 13 207 7 THR H H 8.770 0.01 . 14 207 7 THR N N 115.582 0.2 . 15 208 8 LEU H H 9.142 0.01 . 16 208 8 LEU N N 121.127 0.2 . 17 209 9 THR H H 7.600 0.01 . 18 209 9 THR N N 105.265 0.2 . 19 210 10 GLY H H 7.831 0.01 . 20 210 10 GLY N N 109.317 0.2 . 21 211 11 LYS H H 7.281 0.01 . 22 211 11 LYS N N 121.926 0.2 . 23 212 12 THR H H 8.653 0.01 . 24 212 12 THR N N 120.506 0.2 . 25 213 13 ILE H H 9.579 0.01 . 26 213 13 ILE N N 127.780 0.2 . 27 214 14 THR H H 8.753 0.01 . 28 214 14 THR N N 121.818 0.2 . 29 215 15 LEU H H 8.852 0.01 . 30 215 15 LEU N N 125.422 0.2 . 31 216 16 GLU H H 8.173 0.01 . 32 216 16 GLU N N 121.820 0.2 . 33 217 17 VAL H H 8.775 0.01 . 34 217 17 VAL N N 116.913 0.2 . 35 218 18 GLU H H 8.554 0.01 . 36 218 18 GLU N N 118.302 0.2 . 37 219 19 SER H H 8.962 0.01 . 38 219 19 SER N N 117.343 0.2 . 39 220 20 SER H H 7.313 0.01 . 40 220 20 SER N N 108.858 0.2 . 41 221 21 ASP H H 7.914 0.01 . 42 221 21 ASP N N 123.364 0.2 . 43 222 22 THR H H 7.997 0.01 . 44 222 22 THR N N 108.427 0.2 . 45 223 23 ILE H H 8.705 0.01 . 46 223 23 ILE N N 121.188 0.2 . 47 224 24 ASP H H 9.446 0.01 . 48 224 24 ASP N N 118.121 0.2 . 49 225 25 ASN H H 8.025 0.01 . 50 225 25 ASN N N 119.432 0.2 . 51 226 26 VAL H H 8.106 0.01 . 52 226 26 VAL N N 121.987 0.2 . 53 227 27 LYS H H 8.569 0.01 . 54 227 27 LYS N N 118.784 0.2 . 55 228 28 SER H H 8.131 0.01 . 56 228 28 SER N N 117.318 0.2 . 57 229 29 LYS H H 7.953 0.01 . 58 229 29 LYS N N 123.110 0.2 . 59 230 30 ILE H H 8.339 0.01 . 60 230 30 ILE N N 121.133 0.2 . 61 231 31 GLN H H 8.515 0.01 . 62 231 31 GLN N N 123.491 0.2 . 63 232 32 ASP H H 8.054 0.01 . 64 232 32 ASP N N 119.826 0.2 . 65 233 33 LYS H H 7.471 0.01 . 66 233 33 LYS N N 115.624 0.2 . 67 234 34 GLU H H 8.747 0.01 . 68 234 34 GLU N N 114.311 0.2 . 69 235 35 GLY H H 8.498 0.01 . 70 235 35 GLY N N 108.910 0.2 . 71 236 36 ILE H H 6.160 0.01 . 72 236 36 ILE N N 120.255 0.2 . 73 239 39 ASP H H 8.511 0.01 . 74 239 39 ASP N N 113.438 0.2 . 75 240 40 GLN H H 7.848 0.01 . 76 240 40 GLN N N 116.925 0.2 . 77 241 41 GLN H H 7.503 0.01 . 78 241 41 GLN N N 118.058 0.2 . 79 242 42 ARG H H 8.538 0.01 . 80 242 42 ARG N N 123.225 0.2 . 81 243 43 LEU H H 8.783 0.01 . 82 243 43 LEU N N 124.206 0.2 . 83 244 44 ILE H H 9.159 0.01 . 84 244 44 ILE N N 122.265 0.2 . 85 245 45 PHE H H 8.869 0.01 . 86 245 45 PHE N N 124.841 0.2 . 87 246 46 ALA H H 9.020 0.01 . 88 246 46 ALA N N 100.082 0.2 . 89 247 47 GLY H H 8.161 0.01 . 90 247 47 GLY N N 102.556 0.2 . 91 248 48 LYS H H 7.927 0.01 . 92 248 48 LYS N N 121.402 0.2 . 93 249 49 GLN H H 8.852 0.01 . 94 249 49 GLN N N 123.265 0.2 . 95 250 50 LEU H H 8.580 0.01 . 96 250 50 LEU N N 125.818 0.2 . 97 251 51 GLU H H 8.409 0.01 . 98 251 51 GLU N N 123.007 0.2 . 99 252 52 ASP H H 8.104 0.01 . 100 252 52 ASP N N 120.030 0.2 . 101 253 53 GLY H H 9.528 0.01 . 102 253 53 GLY N N 107.334 0.2 . 103 254 54 ARG H H 7.413 0.01 . 104 254 54 ARG N N 119.164 0.2 . 105 255 55 THR H H 8.870 0.01 . 106 255 55 THR N N 109.051 0.2 . 107 256 56 LEU H H 8.213 0.01 . 108 256 56 LEU N N 117.846 0.2 . 109 257 57 SER H H 8.219 0.01 . 110 257 57 SER N N 112.920 0.2 . 111 258 58 ASP H H 7.937 0.01 . 112 258 58 ASP N N 124.243 0.2 . 113 259 59 TYR H H 7.234 0.01 . 114 259 59 TYR N N 115.535 0.2 . 115 260 60 ASN H H 8.152 0.01 . 116 260 60 ASN N N 116.206 0.2 . 117 261 61 ILE H H 7.258 0.01 . 118 261 61 ILE N N 118.868 0.2 . 119 262 62 GLN H H 7.665 0.01 . 120 262 62 GLN N N 124.908 0.2 . 121 263 63 LYS H H 8.454 0.01 . 122 263 63 LYS N N 120.182 0.2 . 123 264 64 GLU H H 9.246 0.01 . 124 264 64 GLU N N 114.647 0.2 . 125 265 65 SER H H 7.706 0.01 . 126 265 65 SER N N 114.993 0.2 . 127 266 66 THR H H 8.713 0.01 . 128 266 66 THR N N 117.366 0.2 . 129 267 67 LEU H H 9.421 0.01 . 130 267 67 LEU N N 127.582 0.2 . 131 268 68 HIS H H 9.261 0.01 . 132 268 68 HIS N N 119.791 0.2 . 133 269 69 LEU H H 8.292 0.01 . 134 269 69 LEU N N 123.771 0.2 . 135 270 70 VAL H H 9.175 0.01 . 136 270 70 VAL N N 126.591 0.2 . 137 271 71 LEU H H 8.127 0.01 . 138 271 71 LEU N N 122.968 0.2 . 139 272 72 ARG H H 8.515 0.01 . 140 272 72 ARG N N 123.439 0.2 . 141 273 73 LEU H H 8.341 0.01 . 142 273 73 LEU N N 124.677 0.2 . 143 274 74 ARG H H 8.570 0.01 . 144 274 74 ARG N N 122.351 0.2 . 145 275 75 GLY H H 8.487 0.01 . 146 275 75 GLY N N 110.456 0.2 . 147 276 76 GLY H H 7.977 0.01 . 148 276 76 GLY N N 115.825 0.2 . stop_ save_