data_27543 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone assignment of mouse MARCH9 transmembrane domains in LMPG micelles ; _BMRB_accession_number 27543 _BMRB_flat_file_name bmr27543.str _Entry_type original _Submission_date 2018-07-13 _Accession_date 2018-07-13 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Tan Cyrus . . 2 Byrne Eamon FX . 3 Call Melissa J. . 4 Call Matthew E. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 63 "13C chemical shifts" 187 "15N chemical shifts" 63 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2019-02-18 update BMRB 'update entry citation' 2018-12-12 original author 'original release' stop_ _Original_release_date 2018-07-13 save_ ############################# # Citation for this entry # ############################# save_citations_1 _Saveframe_category entry_citation _Citation_full . _Citation_title ; A serine in the first transmembrane domain of the human E3 ubiquitin ligase MARCH9 is critical for down-regulation of its protein substrates ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 30554144 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Tan Cyrus . . 2 Byrne Eamon . . 3 Ah-Cann Casey . . 4 Call Melissa . . 5 Call Matthew . . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_name_full 'The Journal of biological chemistry' _Journal_volume 294 _Journal_issue 7 _Journal_ISSN 1083-351X _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2470 _Page_last 2485 _Year 2019 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name MARCH9-TM _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label MARCH9-TM $MARCH9-TM stop_ _System_molecular_weight 7123 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_MARCH9-TM _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common MARCH9-TM _Molecular_mass 7123 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 65 _Mol_residue_sequence ; IEKVQIAAIVLGSLFLVASI SWLIWSSLSPSAKWQRQDLL FQISYGVYGFVDVVSIGLIV HEGSS ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 180 ILE 2 181 GLU 3 182 LYS 4 183 VAL 5 184 GLN 6 185 ILE 7 186 ALA 8 187 ALA 9 188 ILE 10 189 VAL 11 190 LEU 12 191 GLY 13 192 SER 14 193 LEU 15 194 PHE 16 195 LEU 17 196 VAL 18 197 ALA 19 198 SER 20 199 ILE 21 200 SER 22 201 TRP 23 202 LEU 24 203 ILE 25 204 TRP 26 205 SER 27 206 SER 28 207 LEU 29 208 SER 30 209 PRO 31 210 SER 32 211 ALA 33 212 LYS 34 213 TRP 35 214 GLN 36 215 ARG 37 216 GLN 38 217 ASP 39 218 LEU 40 219 LEU 41 220 PHE 42 221 GLN 43 222 ILE 44 223 SER 45 224 TYR 46 225 GLY 47 226 VAL 48 227 TYR 49 228 GLY 50 229 PHE 51 230 VAL 52 231 ASP 53 232 VAL 54 233 VAL 55 234 SER 56 235 ILE 57 236 GLY 58 237 LEU 59 238 ILE 60 239 VAL 61 240 HIS 62 241 GLU 63 242 GLY 64 243 SER 65 244 SER stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Gene_mnemonic $MARCH9-TM Mouse 10090 Eukaryota Metazoa Mus musculus March9 stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $MARCH9-TM 'recombinant technology' . Escherichia coli . pMM stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type micelle _Details '1 mM peptide in 250 mM lysomyristoyl phosphatidylglycerol (LMPG), 20 mM phosphate buffer (pH 6.8) and 5% D2O.' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling LMPG 250 mM 'natural abundance' 'sodium phosphate' 20 mM 'natural abundance' D2O 5 % '[U-99% 2H]' $MARCH9-TM 1 mM '[U-100% 13C; U-100% 15N; U-80% 2H]' stop_ save_ ############################ # Computer software used # ############################ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version 3.2 loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection processing stop_ _Details . save_ save_XEASY _Saveframe_category software _Name XEASY _Version . loop_ _Vendor _Address _Electronic_address 'Keller and Wuthrich' . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_HNCA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $sample_1 save_ save_3D_HNCO_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HNCACB_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.02 . M pH 6.8 . pH pressure 1 . atm temperature 313 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chem_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D HNCA' '3D HNCO' '3D HNCACB' '3D HN(CO)CA' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chem_shift_reference_1 _Mol_system_component_name MARCH9-TM _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 180 1 ILE C C 168.771 0.2 1 2 180 1 ILE CA C 57.315 0.2 1 3 180 1 ILE CB C 35.58 0.2 1 4 181 2 GLU H H 8.493 0.01 1 5 181 2 GLU C C 175.138 0.2 1 6 181 2 GLU CA C 52.133 0.2 1 7 181 2 GLU CB C 27.477 0.2 1 8 181 2 GLU N N 124.767 0.1 1 9 182 3 LYS H H 9.053 0.01 1 10 182 3 LYS C C 175.751 0.2 1 11 182 3 LYS CA C 57.889 0.2 1 12 182 3 LYS CB C 28.787 0.2 1 13 182 3 LYS N N 123.682 0.1 1 14 183 4 VAL H H 8.689 0.01 1 15 183 4 VAL C C 173.951 0.2 1 16 183 4 VAL CA C 62.598 0.2 1 17 183 4 VAL CB C 27.817 0.2 1 18 183 4 VAL N N 116.371 0.1 1 19 184 5 GLN H H 7.063 0.01 1 20 184 5 GLN C C 175.205 0.2 1 21 184 5 GLN CA C 55.891 0.2 1 22 184 5 GLN CB C 24.712 0.2 1 23 184 5 GLN N N 118.234 0.1 1 24 185 6 ILE H H 7.741 0.01 1 25 185 6 ILE C C 174.824 0.2 1 26 185 6 ILE CA C 61.956 0.2 1 27 185 6 ILE CB C 34.027 0.2 1 28 185 6 ILE N N 118.261 0.1 1 29 186 7 ALA H H 8.278 0.01 1 30 186 7 ALA C C 175.631 0.2 1 31 186 7 ALA CA C 52.371 0.2 1 32 186 7 ALA CB C 14.717 0.2 1 33 186 7 ALA N N 120.701 0.1 1 34 187 8 ALA H H 7.939 0.01 1 35 187 8 ALA C C 176.258 0.2 1 36 187 8 ALA CA C 52.442 0.2 1 37 187 8 ALA CB C 15.008 0.2 1 38 187 8 ALA N N 118.522 0.1 1 39 188 9 ILE H H 7.95 0.01 1 40 188 9 ILE C C 176.258 0.2 1 41 188 9 ILE CA C 62.146 0.2 1 42 188 9 ILE CB C 34.658 0.2 1 43 188 9 ILE N N 117.597 0.1 1 44 189 10 VAL H H 8.544 0.01 1 45 189 10 VAL C C 174.6 0.2 1 46 189 10 VAL CA C 64.263 0.2 1 47 189 10 VAL CB C 27.962 0.2 1 48 189 10 VAL N N 122.155 0.1 1 49 190 11 LEU H H 8.573 0.01 1 50 190 11 LEU C C 176.101 0.2 1 51 190 11 LEU CA C 55.13 0.2 1 52 190 11 LEU CB C 38.005 0.2 1 53 190 11 LEU N N 118.978 0.1 1 54 191 12 GLY H H 8.743 0.01 1 55 191 12 GLY C C 172.406 0.2 1 56 191 12 GLY CA C 44.379 0.2 1 57 191 12 GLY N N 105.823 0.1 1 58 192 13 SER H H 8.228 0.01 1 59 192 13 SER C C 173.1 0.2 1 60 192 13 SER CA C 60.648 0.2 1 61 192 13 SER CB C 59.741 0.2 1 62 192 13 SER N N 118.308 0.1 1 63 193 14 LEU H H 8.138 0.01 1 64 193 14 LEU C C 175.81 0.2 1 65 193 14 LEU CA C 55.011 0.2 1 66 193 14 LEU CB C 37.957 0.2 1 67 193 14 LEU N N 122.417 0.1 1 68 194 15 PHE H H 8.436 0.01 1 69 194 15 PHE C C 176.011 0.2 1 70 194 15 PHE CA C 58.174 0.2 1 71 194 15 PHE CB C 35.482 0.2 1 72 194 15 PHE N N 118.723 0.1 1 73 195 16 LEU H H 8.413 0.01 1 74 195 16 LEU C C 175.586 0.2 1 75 195 16 LEU CA C 55.391 0.2 1 76 195 16 LEU CB C 38.248 0.2 1 77 195 16 LEU N N 122.35 0.1 1 78 196 17 VAL H H 8.45 0.01 1 79 196 17 VAL C C 176.437 0.2 1 80 196 17 VAL CA C 63.882 0.2 1 81 196 17 VAL CB C 28.011 0.2 1 82 196 17 VAL N N 118.214 0.1 1 83 197 18 ALA H H 8.806 0.01 1 84 197 18 ALA C C 175.854 0.2 1 85 197 18 ALA CA C 52.561 0.2 1 86 197 18 ALA CB C 15.008 0.2 1 87 197 18 ALA N N 124.225 0.1 1 88 198 19 SER H H 8.173 0.01 1 89 198 19 SER C C 173.525 0.2 1 90 198 19 SER CA C 60.315 0.2 1 91 198 19 SER N N 114.291 0.1 1 92 199 20 ILE H H 8.298 0.01 1 93 199 20 ILE C C 175.227 0.2 1 94 199 20 ILE CA C 62.051 0.2 1 95 199 20 ILE CB C 34.173 0.2 1 96 199 20 ILE N N 121.844 0.1 1 97 200 21 SER H H 8.263 0.01 1 98 200 21 SER C C 172.921 0.2 1 99 200 21 SER CA C 60.933 0.2 1 100 200 21 SER CB C 59.79 0.2 1 101 200 21 SER N N 116.947 0.1 1 102 201 22 TRP H H 8.485 0.01 1 103 201 22 TRP C C 176.168 0.2 1 104 201 22 TRP CA C 58.293 0.2 1 105 201 22 TRP CB C 25.925 0.2 1 106 201 22 TRP N N 122.604 0.1 1 107 202 23 LEU H H 8.448 0.01 1 108 202 23 LEU C C 176.817 0.2 1 109 202 23 LEU CA C 55.368 0.2 1 110 202 23 LEU CB C 38.297 0.2 1 111 202 23 LEU N N 122.082 0.1 1 112 203 24 ILE H H 8.519 0.01 1 113 203 24 ILE C C 175.227 0.2 1 114 203 24 ILE CA C 62.455 0.2 1 115 203 24 ILE CB C 34.221 0.2 1 116 203 24 ILE N N 120.165 0.1 1 117 204 25 TRP H H 8.791 0.01 1 118 204 25 TRP C C 175.496 0.2 1 119 204 25 TRP CA C 58.055 0.2 1 120 204 25 TRP CB C 26.361 0.2 1 121 204 25 TRP N N 121.512 0.1 1 122 205 26 SER H H 8.479 0.01 1 123 205 26 SER C C 172.742 0.2 1 124 205 26 SER CA C 57.96 0.2 1 125 205 26 SER CB C 59.984 0.2 1 126 205 26 SER N N 112.227 0.1 1 127 206 27 SER H H 7.52 0.01 1 128 206 27 SER C C 171.958 0.2 1 129 206 27 SER CA C 57.461 0.2 1 130 206 27 SER CB C 60.906 0.2 1 131 206 27 SER N N 116.337 0.1 1 132 207 28 LEU H H 7.504 0.01 1 133 207 28 LEU C C 174.365 0.2 1 134 207 28 LEU CA C 52.157 0.2 1 135 207 28 LEU CB C 39.364 0.2 1 136 207 28 LEU N N 120.802 0.1 1 137 208 29 SER H H 7.749 0.01 1 138 208 29 SER CA C 53.156 0.2 1 139 208 29 SER CB C 60.469 0.2 1 140 208 29 SER N N 115.368 0.1 1 141 209 30 PRO C C 174.892 0.2 1 142 209 30 PRO CA C 61.127 0.2 1 143 209 30 PRO CB C 28.351 0.2 1 144 210 31 SER H H 7.874 0.01 1 145 210 31 SER C C 171.868 0.2 1 146 210 31 SER CA C 55.534 0.2 1 147 210 31 SER CB C 60.517 0.2 1 148 210 31 SER N N 113.267 0.1 1 149 211 32 ALA H H 7.725 0.01 1 150 211 32 ALA C C 175.227 0.2 1 151 211 32 ALA CA C 49.826 0.2 1 152 211 32 ALA CB C 15.784 0.2 1 153 211 32 ALA N N 125.558 0.1 1 154 212 33 LYS H H 7.965 0.01 1 155 212 33 LYS C C 174.041 0.2 1 156 212 33 LYS CA C 54.25 0.2 1 157 212 33 LYS CB C 29.224 0.2 1 158 212 33 LYS N N 119.514 0.1 1 159 213 34 TRP H H 7.672 0.01 1 160 213 34 TRP C C 173.593 0.2 1 161 213 34 TRP CA C 53.893 0.2 1 162 213 34 TRP CB C 26.555 0.2 1 163 213 34 TRP N N 119.48 0.1 1 164 214 35 GLN H H 8.224 0.01 1 165 214 35 GLN C C 174.018 0.2 1 166 214 35 GLN CA C 52.894 0.2 1 167 214 35 GLN CB C 25.925 0.2 1 168 214 35 GLN N N 120.768 0.1 1 169 215 36 ARG H H 8.087 0.01 1 170 215 36 ARG C C 174.085 0.2 1 171 215 36 ARG CA C 53.893 0.2 1 172 215 36 ARG CB C 26.604 0.2 1 173 215 36 ARG N N 121.163 0.1 1 174 216 37 GLN H H 8.52 0.01 1 175 216 37 GLN C C 174.108 0.2 1 176 216 37 GLN CA C 55.368 0.2 1 177 216 37 GLN CB C 24.954 0.2 1 178 216 37 GLN N N 119.85 0.1 1 179 217 38 ASP H H 8.16 0.01 1 180 217 38 ASP C C 175.317 0.2 1 181 217 38 ASP CA C 53.179 0.2 1 182 217 38 ASP CB C 37.181 0.2 1 183 217 38 ASP N N 118.931 0.1 1 184 218 39 LEU H H 7.786 0.01 1 185 218 39 LEU C C 175.631 0.2 1 186 218 39 LEU CA C 54.678 0.2 1 187 218 39 LEU CB C 38.588 0.2 1 188 218 39 LEU N N 121.244 0.1 1 189 219 40 LEU H H 7.91 0.01 1 190 219 40 LEU C C 176.571 0.2 1 191 219 40 LEU CA C 54.63 0.2 1 192 219 40 LEU CB C 37.714 0.2 1 193 219 40 LEU N N 117.899 0.1 1 194 220 41 PHE H H 8.009 0.01 1 195 220 41 PHE C C 174.668 0.2 1 196 220 41 PHE CA C 57.389 0.2 1 197 220 41 PHE CB C 35.24 0.2 1 198 220 41 PHE N N 119.427 0.1 1 199 221 42 GLN H H 8.119 0.01 1 200 221 42 GLN C C 176.258 0.2 1 201 221 42 GLN CA C 55.629 0.2 1 202 221 42 GLN CB C 25.148 0.2 1 203 221 42 GLN N N 118.904 0.1 1 204 222 43 ILE H H 8.266 0.01 1 205 222 43 ILE C C 174.511 0.2 1 206 222 43 ILE CA C 61.385 0.2 1 207 222 43 ILE CB C 34.658 0.2 1 208 222 43 ILE N N 118.462 0.1 1 209 223 44 SER H H 7.995 0.01 1 210 223 44 SER C C 172.563 0.2 1 211 223 44 SER CA C 59.03 0.2 1 212 223 44 SER CB C 60.129 0.2 1 213 223 44 SER N N 116.344 0.1 1 214 224 45 TYR H H 8.116 0.01 1 215 224 45 TYR C C 175.966 0.2 1 216 224 45 TYR CA C 57.318 0.2 1 217 224 45 TYR CB C 34.706 0.2 1 218 224 45 TYR N N 121.251 0.1 1 219 225 46 GLY H H 8.113 0.01 1 220 225 46 GLY C C 172.675 0.2 1 221 225 46 GLY CA C 43.547 0.2 1 222 225 46 GLY N N 108.163 0.1 1 223 226 47 VAL H H 8.199 0.01 1 224 226 47 VAL C C 174.466 0.2 1 225 226 47 VAL CA C 62.646 0.2 1 226 226 47 VAL CB C 28.205 0.2 1 227 226 47 VAL N N 120.58 0.1 1 228 227 48 TYR H H 8.113 0.01 1 229 227 48 TYR C C 174.802 0.2 1 230 227 48 TYR CA C 57.461 0.2 1 231 227 48 TYR CB C 34.706 0.2 1 232 227 48 TYR N N 119.574 0.1 1 233 228 49 GLY H H 8.006 0.01 1 234 228 49 GLY C C 172.563 0.2 1 235 228 49 GLY CA C 43.238 0.2 1 236 228 49 GLY N N 105.949 0.1 1 237 229 50 PHE H H 7.942 0.01 1 238 229 50 PHE C C 173.839 0.2 1 239 229 50 PHE CA C 56.866 0.2 1 240 229 50 PHE CB C 36.695 0.2 1 241 229 50 PHE N N 120.533 0.1 1 242 230 51 VAL H H 7.933 0.01 1 243 230 51 VAL C C 173.772 0.2 1 244 230 51 VAL CA C 61.647 0.2 1 245 230 51 VAL CB C 28.35 0.2 1 246 230 51 VAL N N 117.503 0.1 1 247 231 52 ASP H H 7.975 0.01 1 248 231 52 ASP C C 174.511 0.2 1 249 231 52 ASP CA C 52.49 0.2 1 250 231 52 ASP CB C 37.278 0.2 1 251 231 52 ASP N N 120.513 0.1 1 252 232 53 VAL H H 7.712 0.01 1 253 232 53 VAL C C 174.6 0.2 1 254 232 53 VAL CA C 61.385 0.2 1 255 232 53 VAL CB C 28.593 0.2 1 256 232 53 VAL N N 117.845 0.1 1 257 233 54 VAL H H 7.75 0.01 1 258 233 54 VAL C C 174.018 0.2 1 259 233 54 VAL CA C 61.171 0.2 1 260 233 54 VAL CB C 28.35 0.2 1 261 233 54 VAL N N 118.824 0.1 1 262 234 55 SER H H 7.868 0.01 1 263 234 55 SER C C 172.876 0.2 1 264 234 55 SER CA C 56.89 0.2 1 265 234 55 SER CB C 60.469 0.2 1 266 234 55 SER N N 116.116 0.1 1 267 235 56 ILE H H 7.859 0.01 1 268 235 56 ILE C C 174.399 0.2 1 269 235 56 ILE CA C 59.839 0.2 1 270 235 56 ILE CB C 34.561 0.2 1 271 235 56 ILE N N 121.237 0.1 1 272 236 57 GLY H H 8.144 0.01 1 273 236 57 GLY C C 171.913 0.2 1 274 236 57 GLY CA C 43.119 0.2 1 275 236 57 GLY N N 108.759 0.1 1 276 237 58 LEU H H 7.708 0.01 1 277 237 58 LEU C C 174.712 0.2 1 278 237 58 LEU CA C 53.298 0.2 1 279 237 58 LEU CB C 39.17 0.2 1 280 237 58 LEU N N 119.997 0.1 1 281 238 59 ILE H H 7.647 0.01 1 282 238 59 ILE C C 173.503 0.2 1 283 238 59 ILE CA C 58.745 0.2 1 284 238 59 ILE CB C 35.191 0.2 1 285 238 59 ILE N N 117.792 0.1 1 286 239 60 VAL H H 7.896 0.01 1 287 239 60 VAL C C 173.167 0.2 1 288 239 60 VAL CA C 59.435 0.2 1 289 239 60 VAL CB C 28.981 0.2 1 290 239 60 VAL N N 120.942 0.1 1 291 240 61 HIS H H 8.317 0.01 1 292 240 61 HIS C C 171.846 0.2 1 293 240 61 HIS CA C 52.204 0.2 1 294 240 61 HIS CB C 26.119 0.2 1 295 240 61 HIS N N 121.619 0.1 1 296 241 62 GLU H H 8.433 0.01 1 297 241 62 GLU C C 174.287 0.2 1 298 241 62 GLU CA C 53.631 0.2 1 299 241 62 GLU CB C 26.555 0.2 1 300 241 62 GLU N N 122.959 0.1 1 301 242 63 GLY H H 8.413 0.01 1 302 242 63 GLY C C 171.443 0.2 1 303 242 63 GLY CA C 42.191 0.2 1 304 242 63 GLY N N 110.732 0.1 1 305 243 64 SER H H 8.166 0.01 1 306 243 64 SER C C 171.219 0.2 1 307 243 64 SER CA C 55.249 0.2 1 308 243 64 SER CB C 61.391 0.2 1 309 243 64 SER N N 116.398 0.1 1 310 244 65 SER H H 7.972 0.01 1 311 244 65 SER CA C 56.985 0.2 1 312 244 65 SER CB C 61.924 0.2 1 313 244 65 SER N N 123.662 0.1 1 stop_ save_