data_27322 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; C-terminal domain of Cdc37 cochaperone. Y298E phosphomimetic mutant. ; _BMRB_accession_number 27322 _BMRB_flat_file_name bmr27322.str _Entry_type original _Submission_date 2017-12-03 _Accession_date 2017-12-03 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'phosphomimetic mutant' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bachman Ashleigh . . 2 Keramisanou Dimitra . . 3 'Kumar M. V.' Vaasantha . . 4 Gelis Ioannis . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 76 "13C chemical shifts" 226 "15N chemical shifts" 76 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2018-03-22 update BMRB 'update entry citation' 2018-01-16 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 25740 'C-terminal domain of Cdc37 cochaperone' 27323 'C-terminal domain of Cdc37 (Y298F mutant)' 27324 'C-terminal domain of Cdc37 (unfolded)' stop_ _Original_release_date 2017-12-04 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Phosphorylation induced cochaperone unfolding promotes kinase recruitment and client class-specific Hsp90 phosphorylation ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 29343704 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Bachman Ashleigh B. . 2 Keramisanou Dimitra . . 3 Xu Wanping . . 4 Beebe Kristin . . 5 Moses Michael A. . 6 'Vasantha Kumar' M V. . 7 Gray Geoffrey . . 8 Noor 'Radwan Ebna' E. . 9 'van der Vaart' Arjan . . 10 Neckers Len . . 11 Gelis Ioannis . . stop_ _Journal_abbreviation 'Nat. Commun.' _Journal_name_full 'Nature communications' _Journal_volume 9 _Journal_issue 1 _Journal_ISSN 2041-1723 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 265 _Page_last 265 _Year 2018 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Cdc37 C-terminal domain' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label Cdc37 $Cdc37_C-terminal_domain stop_ _System_molecular_weight 9800 _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . loop_ _Biological_function cochaperone stop_ _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Cdc37_C-terminal_domain _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Cdc37_C-terminal_domain _Molecular_mass . _Mol_thiol_state 'all free' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 93 _Mol_residue_sequence ; GMGPGGLDPVEVEESLPEEL QKCFDVKDVQMLQDAISKMD PTDAKYHMQRCIDSGLWVPN SKASEAKEGEEAGPGDPLLE AVPKTGDEKDVSV ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 286 GLY 2 287 MET 3 288 GLY 4 289 PRO 5 290 GLY 6 291 GLY 7 292 LEU 8 293 ASP 9 294 PRO 10 295 VAL 11 296 GLU 12 297 VAL 13 298 GLU 14 299 GLU 15 300 SER 16 301 LEU 17 302 PRO 18 303 GLU 19 304 GLU 20 305 LEU 21 306 GLN 22 307 LYS 23 308 CYS 24 309 PHE 25 310 ASP 26 311 VAL 27 312 LYS 28 313 ASP 29 314 VAL 30 315 GLN 31 316 MET 32 317 LEU 33 318 GLN 34 319 ASP 35 320 ALA 36 321 ILE 37 322 SER 38 323 LYS 39 324 MET 40 325 ASP 41 326 PRO 42 327 THR 43 328 ASP 44 329 ALA 45 330 LYS 46 331 TYR 47 332 HIS 48 333 MET 49 334 GLN 50 335 ARG 51 336 CYS 52 337 ILE 53 338 ASP 54 339 SER 55 340 GLY 56 341 LEU 57 342 TRP 58 343 VAL 59 344 PRO 60 345 ASN 61 346 SER 62 347 LYS 63 348 ALA 64 349 SER 65 350 GLU 66 351 ALA 67 352 LYS 68 353 GLU 69 354 GLY 70 355 GLU 71 356 GLU 72 357 ALA 73 358 GLY 74 359 PRO 75 360 GLY 76 361 ASP 77 362 PRO 78 363 LEU 79 364 LEU 80 365 GLU 81 366 ALA 82 367 VAL 83 368 PRO 84 369 LYS 85 370 THR 86 371 GLY 87 372 ASP 88 373 GLU 89 374 LYS 90 375 ASP 91 376 VAL 92 377 SER 93 378 VAL stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value UNP Q16543 . . . . . . stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Cdc37_C-terminal_domain Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Cdc37_C-terminal_domain 'recombinant technology' . Escherichia coli . PDB.His.MBP stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_15N-13C _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Cdc37_C-terminal_domain 0.5 mM '[U-100% 13C; U-100% 15N]' stop_ save_ save_methyl-labeled _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Cdc37_C-terminal_domain 0.5 mM methyl-labeled stop_ save_ save_15N _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Cdc37_C-terminal_domain 0.5 mM '[U-100% 15N]' stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address 'T. D. Goddard and D. G. Kneller' 'University of California, San Francisco' . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Agilent _Model 'direct drive' _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_HNCA_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCA' _Sample_label $15N-13C save_ save_3D_HN(CO)CA_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $15N-13C save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $15N-13C save_ save_3D_CBCA(CO)NH_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $15N-13C save_ save_3D_1H-15N_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $15N save_ save_2D_1H-15N_HSQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $15N save_ save_2D_1H-13C_HMQC_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HMQC' _Sample_label $methyl-labeled save_ save_CH3-CH3_HMQC-NOESY-HMQC_8 _Saveframe_category NMR_applied_experiment _Experiment_name 'CH3-CH3 HMQC-NOESY-HMQC' _Sample_label $methyl-labeled save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.1 . M pH 7.5 . pH pressure 1 . atm temperature 273 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water H 1 protons ppm 4.7 external direct . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D HNCA' '3D HN(CO)CA' '3D HNCACB' '3D CBCA(CO)NH' '3D 1H-15N NOESY' '2D 1H-15N HSQC' stop_ loop_ _Sample_label $15N-13C $15N stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name Cdc37 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 288 3 GLY H H 8.221 . . 2 288 3 GLY CA C 44.522 . . 3 288 3 GLY N N 110.324 . . 4 289 4 PRO C C 177.098 . . 5 289 4 PRO CA C 62.897 . . 6 289 4 PRO CB C 34.520 . . 7 290 5 GLY H H 8.537 . . 8 290 5 GLY C C 174.627 . . 9 290 5 GLY CA C 45.295 . . 10 290 5 GLY N N 110.258 . . 11 291 6 GLY H H 8.145 . . 12 291 6 GLY C C 173.795 . . 13 291 6 GLY CA C 45.332 . . 14 291 6 GLY N N 108.368 . . 15 292 7 LEU H H 7.952 . . 16 292 7 LEU C C 176.665 . . 17 292 7 LEU CA C 54.719 . . 18 292 7 LEU CB C 42.890 . . 19 292 7 LEU N N 121.161 . . 20 293 8 ASP H H 8.297 . . 21 293 8 ASP CA C 52.627 . . 22 293 8 ASP CB C 40.994 . . 23 293 8 ASP N N 122.991 . . 24 294 9 PRO C C 176.981 . . 25 294 9 PRO CA C 63.386 . . 26 294 9 PRO CB C 32.040 . . 27 295 10 VAL H H 8.155 . . 28 295 10 VAL C C 176.353 . . 29 295 10 VAL CA C 62.730 . . 30 295 10 VAL CB C 32.631 . . 31 295 10 VAL N N 119.854 . . 32 296 11 GLU H H 8.222 . . 33 296 11 GLU C C 177.309 . . 34 296 11 GLU CA C 56.660 . . 35 296 11 GLU CB C 30.396 . . 36 296 11 GLU N N 123.878 . . 37 297 12 VAL H H 8.101 . . 38 297 12 VAL C C 176.034 . . 39 297 12 VAL CA C 62.479 . . 40 297 12 VAL CB C 33.020 . . 41 297 12 VAL N N 121.122 . . 42 298 13 GLU H H 8.433 . . 43 298 13 GLU C C 176.241 . . 44 298 13 GLU CA C 56.592 . . 45 298 13 GLU CB C 30.446 . . 46 298 13 GLU N N 124.564 . . 47 299 14 GLU H H 8.414 . . 48 299 14 GLU C C 176.170 . . 49 299 14 GLU CA C 56.553 . . 50 299 14 GLU CB C 30.611 . . 51 299 14 GLU N N 122.741 . . 52 300 15 SER H H 8.319 . . 53 300 15 SER C C 173.957 . . 54 300 15 SER CA C 58.035 . . 55 300 15 SER CB C 63.944 . . 56 300 15 SER N N 117.519 . . 57 301 16 LEU H H 8.358 . . 58 301 16 LEU CA C 53.236 . . 59 301 16 LEU CB C 41.911 . . 60 301 16 LEU N N 125.387 . . 61 302 17 PRO C C 177.422 . . 62 302 17 PRO CA C 63.309 . . 63 302 17 PRO CB C 32.168 . . 64 303 18 GLU H H 8.674 . . 65 303 18 GLU C C 177.377 . . 66 303 18 GLU CA C 58.358 . . 67 303 18 GLU CB C 30.101 . . 68 303 18 GLU N N 122.276 . . 69 304 19 GLU H H 8.753 . . 70 304 19 GLU CA C 57.807 . . 71 304 19 GLU CB C 29.782 . . 72 304 19 GLU N N 119.689 . . 73 305 20 LEU H H 7.834 . . 74 305 20 LEU C C 177.621 . . 75 305 20 LEU CA C 55.772 . . 76 305 20 LEU CB C 42.330 . . 77 305 20 LEU N N 120.533 . . 78 306 21 GLN H H 8.091 . . 79 306 21 GLN CA C 57.034 . . 80 306 21 GLN CB C 28.975 . . 81 306 21 GLN N N 119.924 . . 82 307 22 LYS H H 8.072 . . 83 307 22 LYS CA C 57.043 . . 84 307 22 LYS CB C 32.787 . . 85 307 22 LYS N N 120.130 . . 86 310 25 ASP CA C 54.605 . . 87 310 25 ASP CB C 41.568 . . 88 311 26 VAL H H 7.912 . . 89 311 26 VAL C C 176.202 . . 90 311 26 VAL CA C 62.643 . . 91 311 26 VAL CB C 32.422 . . 92 311 26 VAL N N 118.746 . . 93 312 27 LYS H H 8.224 . . 94 312 27 LYS C C 176.318 . . 95 312 27 LYS CA C 56.972 . . 96 312 27 LYS CB C 32.258 . . 97 312 27 LYS N N 122.355 . . 98 313 28 ASP H H 7.929 . . 99 313 28 ASP C C 176.526 . . 100 313 28 ASP CA C 54.448 . . 101 313 28 ASP CB C 41.597 . . 102 313 28 ASP N N 120.094 . . 103 314 29 VAL H H 8.084 . . 104 314 29 VAL C C 176.820 . . 105 314 29 VAL CA C 64.097 . . 106 314 29 VAL CB C 32.268 . . 107 314 29 VAL N N 120.875 . . 108 315 30 GLN H H 8.295 . . 109 315 30 GLN C C 176.996 . . 110 315 30 GLN CA C 57.547 . . 111 315 30 GLN CB C 28.774 . . 112 315 30 GLN N N 122.156 . . 113 316 31 MET H H 8.166 . . 114 316 31 MET C C 177.450 . . 115 316 31 MET CA C 57.033 . . 116 316 31 MET CB C 32.733 . . 117 316 31 MET N N 120.017 . . 118 317 32 LEU H H 7.966 . . 119 317 32 LEU C C 177.701 . . 120 317 32 LEU CA C 56.711 . . 121 317 32 LEU CB C 42.059 . . 122 317 32 LEU N N 122.057 . . 123 318 33 GLN H H 8.233 . . 124 318 33 GLN CA C 57.675 . . 125 318 33 GLN CB C 28.897 . . 126 318 33 GLN N N 119.193 . . 127 319 34 ASP H H 8.218 . . 128 319 34 ASP C C 177.063 . . 129 319 34 ASP CA C 55.918 . . 130 319 34 ASP CB C 41.104 . . 131 319 34 ASP N N 120.426 . . 132 320 35 ALA H H 8.011 . . 133 320 35 ALA C C 179.354 . . 134 320 35 ALA CA C 54.072 . . 135 320 35 ALA CB C 18.837 . . 136 320 35 ALA N N 123.005 . . 137 321 36 ILE H H 8.123 . . 138 321 36 ILE C C 177.510 . . 139 321 36 ILE CA C 62.855 . . 140 321 36 ILE CB C 38.267 . . 141 321 36 ILE N N 118.258 . . 142 322 37 SER H H 8.042 . . 143 322 37 SER C C 174.657 . . 144 322 37 SER CA C 59.934 . . 145 322 37 SER CB C 63.546 . . 146 322 37 SER N N 117.434 . . 147 323 38 LYS H H 7.717 . . 148 323 38 LYS CA C 56.238 . . 149 323 38 LYS CB C 33.145 . . 150 323 38 LYS N N 121.053 . . 151 324 39 MET H H 7.818 . . 152 324 39 MET C C 175.560 . . 153 324 39 MET CA C 55.692 . . 154 324 39 MET CB C 34.034 . . 155 324 39 MET N N 120.199 . . 156 325 40 ASP H H 8.619 . . 157 325 40 ASP CA C 52.564 . . 158 325 40 ASP CB C 41.559 . . 159 325 40 ASP N N 123.763 . . 160 326 41 PRO C C 177.987 . . 161 326 41 PRO CA C 62.335 . . 162 326 41 PRO CB C 32.138 . . 163 327 42 THR H H 8.268 . . 164 327 42 THR C C 175.336 . . 165 327 42 THR CA C 64.106 . . 166 327 42 THR CB C 69.076 . . 167 327 42 THR N N 114.329 . . 168 328 43 ASP H H 7.921 . . 169 328 43 ASP C C 177.166 . . 170 328 43 ASP CA C 56.881 . . 171 328 43 ASP CB C 41.446 . . 172 328 43 ASP N N 123.045 . . 173 329 44 ALA H H 8.427 . . 174 329 44 ALA C C 178.870 . . 175 329 44 ALA CA C 54.572 . . 176 329 44 ALA CB C 18.991 . . 177 329 44 ALA N N 124.090 . . 178 330 45 LYS H H 8.070 . . 179 330 45 LYS C C 177.670 . . 180 330 45 LYS CA C 58.371 . . 181 330 45 LYS CB C 32.311 . . 182 330 45 LYS N N 117.680 . . 183 331 46 TYR H H 7.708 . . 184 331 46 TYR C C 176.842 . . 185 331 46 TYR CA C 59.564 . . 186 331 46 TYR CB C 38.408 . . 187 331 46 TYR N N 119.231 . . 188 332 47 HIS H H 8.092 . . 189 332 47 HIS C C 176.028 . . 190 332 47 HIS CA C 58.452 . . 191 332 47 HIS CB C 31.057 . . 192 332 47 HIS N N 119.030 . . 193 333 48 MET H H 8.302 . . 194 333 48 MET C C 176.946 . . 195 333 48 MET CA C 57.371 . . 196 333 48 MET CB C 32.628 . . 197 333 48 MET N N 119.586 . . 198 334 49 GLN H H 8.158 . . 199 334 49 GLN CA C 57.370 . . 200 334 49 GLN CB C 28.857 . . 201 334 49 GLN N N 119.387 . . 202 335 50 ARG H H 8.160 . . 203 335 50 ARG N N 119.905 . . 204 336 51 CYS C C 175.684 . . 205 336 51 CYS CA C 61.185 . . 206 336 51 CYS CB C 27.600 . . 207 337 52 ILE H H 8.172 . . 208 337 52 ILE C C 173.754 . . 209 337 52 ILE CA C 63.177 . . 210 337 52 ILE CB C 38.522 . . 211 337 52 ILE N N 122.701 . . 212 338 53 ASP H H 8.535 . . 213 338 53 ASP C C 177.010 . . 214 338 53 ASP CA C 55.823 . . 215 338 53 ASP CB C 40.910 . . 216 338 53 ASP N N 122.674 . . 217 339 54 SER H H 8.014 . . 218 339 54 SER C C 175.063 . . 219 339 54 SER CA C 59.101 . . 220 339 54 SER CB C 64.107 . . 221 339 54 SER N N 114.514 . . 222 340 55 GLY H H 8.154 . . 223 340 55 GLY C C 174.053 . . 224 340 55 GLY CA C 45.735 . . 225 340 55 GLY N N 110.208 . . 226 341 56 LEU H H 7.855 . . 227 341 56 LEU CA C 55.374 . . 228 341 56 LEU CB C 42.649 . . 229 341 56 LEU N N 120.651 . . 230 342 57 TRP H H 7.894 . . 231 342 57 TRP C C 175.027 . . 232 342 57 TRP CA C 57.039 . . 233 342 57 TRP CB C 30.059 . . 234 342 57 TRP N N 120.499 . . 235 343 58 VAL H H 7.718 . . 236 343 58 VAL CA C 59.156 . . 237 343 58 VAL CB C 33.067 . . 238 343 58 VAL N N 123.758 . . 239 347 62 LYS C C 176.454 . . 240 347 62 LYS CA C 58.118 . . 241 348 63 ALA H H 8.253 . . 242 348 63 ALA C C 177.907 . . 243 348 63 ALA CA C 52.884 . . 244 348 63 ALA CB C 19.182 . . 245 348 63 ALA N N 125.245 . . 246 349 64 SER H H 8.200 . . 247 349 64 SER C C 174.522 . . 248 349 64 SER CA C 58.552 . . 249 349 64 SER CB C 63.766 . . 250 349 64 SER N N 114.699 . . 251 350 65 GLU H H 8.281 . . 252 350 65 GLU C C 176.007 . . 253 350 65 GLU CA C 56.507 . . 254 350 65 GLU CB C 30.407 . . 255 350 65 GLU N N 122.770 . . 256 351 66 ALA H H 8.196 . . 257 351 66 ALA C C 177.433 . . 258 351 66 ALA CA C 52.495 . . 259 351 66 ALA CB C 19.261 . . 260 351 66 ALA N N 125.070 . . 261 352 67 LYS H H 8.292 . . 262 352 67 LYS C C 176.475 . . 263 352 67 LYS CA C 55.993 . . 264 352 67 LYS CB C 33.250 . . 265 352 67 LYS N N 121.407 . . 266 353 68 GLU H H 8.462 . . 267 353 68 GLU C C 176.978 . . 268 353 68 GLU CA C 56.947 . . 269 353 68 GLU CB C 30.278 . . 270 353 68 GLU N N 122.528 . . 271 354 69 GLY H H 8.489 . . 272 354 69 GLY C C 174.153 . . 273 354 69 GLY CA C 45.371 . . 274 354 69 GLY N N 110.562 . . 275 355 70 GLU H H 8.167 . . 276 355 70 GLU C C 176.526 . . 277 355 70 GLU CA C 56.471 . . 278 355 70 GLU CB C 30.537 . . 279 355 70 GLU N N 120.438 . . 280 356 71 GLU H H 8.501 . . 281 356 71 GLU C C 176.095 . . 282 356 71 GLU CA C 56.568 . . 283 356 71 GLU CB C 30.308 . . 284 356 71 GLU N N 122.064 . . 285 357 72 ALA H H 8.328 . . 286 357 72 ALA C C 177.849 . . 287 357 72 ALA CA C 52.615 . . 288 357 72 ALA CB C 19.552 . . 289 357 72 ALA N N 125.493 . . 290 358 73 GLY H H 8.232 . . 291 358 73 GLY CA C 44.551 . . 292 358 73 GLY N N 108.462 . . 293 359 74 PRO C C 177.573 . . 294 359 74 PRO CA C 63.425 . . 295 359 74 PRO CB C 32.176 . . 296 360 75 GLY H H 8.393 . . 297 360 75 GLY C C 173.414 . . 298 360 75 GLY CA C 44.891 . . 299 360 75 GLY N N 109.267 . . 300 361 76 ASP H H 8.107 . . 301 361 76 ASP CA C 52.334 . . 302 361 76 ASP CB C 41.322 . . 303 361 76 ASP N N 121.813 . . 304 362 77 PRO C C 177.222 . . 305 362 77 PRO CA C 63.580 . . 306 362 77 PRO CB C 32.155 . . 307 363 78 LEU H H 8.351 . . 308 363 78 LEU C C 177.621 . . 309 363 78 LEU CA C 55.491 . . 310 363 78 LEU CB C 41.722 . . 311 363 78 LEU N N 120.767 . . 312 364 79 LEU H H 7.891 . . 313 364 79 LEU CA C 55.254 . . 314 364 79 LEU CB C 42.363 . . 315 364 79 LEU N N 121.917 . . 316 365 80 GLU H H 8.097 . . 317 365 80 GLU C C 175.862 . . 318 365 80 GLU CA C 56.388 . . 319 365 80 GLU CB C 30.473 . . 320 365 80 GLU N N 121.026 . . 321 366 81 ALA H H 8.148 . . 322 366 81 ALA C C 177.280 . . 323 366 81 ALA CA C 52.340 . . 324 366 81 ALA CB C 19.294 . . 325 366 81 ALA N N 124.965 . . 326 367 82 VAL H H 8.008 . . 327 367 82 VAL CA C 59.880 . . 328 367 82 VAL CB C 32.597 . . 329 367 82 VAL N N 120.962 . . 330 368 83 PRO CA C 63.115 . . 331 368 83 PRO CB C 32.252 . . 332 369 84 LYS H H 8.448 . . 333 369 84 LYS C C 176.925 . . 334 369 84 LYS CA C 56.056 . . 335 369 84 LYS CB C 33.186 . . 336 369 84 LYS N N 122.344 . . 337 370 85 THR H H 8.341 . . 338 370 85 THR CA C 61.820 . . 339 370 85 THR CB C 70.026 . . 340 370 85 THR N N 115.412 . . 341 371 86 GLY H H 8.520 . . 342 371 86 GLY C C 173.834 . . 343 371 86 GLY CA C 45.390 . . 344 371 86 GLY N N 111.294 . . 345 372 87 ASP H H 8.249 . . 346 372 87 ASP C C 176.385 . . 347 372 87 ASP CA C 54.330 . . 348 372 87 ASP CB C 41.415 . . 349 372 87 ASP N N 120.624 . . 350 373 88 GLU H H 8.497 . . 351 373 88 GLU C C 176.545 . . 352 373 88 GLU CA C 57.120 . . 353 373 88 GLU CB C 29.940 . . 354 373 88 GLU N N 121.317 . . 355 374 89 LYS H H 8.270 . . 356 374 89 LYS C C 176.180 . . 357 374 89 LYS CA C 56.295 . . 358 374 89 LYS CB C 33.092 . . 359 374 89 LYS N N 121.533 . . 360 375 90 ASP H H 8.285 . . 361 375 90 ASP C C 176.073 . . 362 375 90 ASP CA C 54.414 . . 363 375 90 ASP CB C 41.175 . . 364 375 90 ASP N N 121.473 . . 365 376 91 VAL H H 7.990 . . 366 376 91 VAL C C 176.036 . . 367 376 91 VAL CA C 61.991 . . 368 376 91 VAL CB C 32.756 . . 369 376 91 VAL N N 119.590 . . 370 377 92 SER H H 8.395 . . 371 377 92 SER C C 173.541 . . 372 377 92 SER CA C 58.424 . . 373 377 92 SER CB C 63.991 . . 374 377 92 SER N N 120.203 . . 375 378 93 VAL H H 7.692 . . 376 378 93 VAL CA C 63.611 . . 377 378 93 VAL CB C 33.258 . . 378 378 93 VAL N N 125.726 . . stop_ save_