data_27044 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Backbone 1H, 13C, and 15N Chemical Shift Assignments for Sgt2_TPR ; _BMRB_accession_number 27044 _BMRB_flat_file_name bmr27044.str _Entry_type original _Submission_date 2017-03-06 _Accession_date 2017-03-06 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Krysztofinska Ewelina M. . 2 Evans Nicola J. . 3 Thapaliya Arjun . . 4 Murray James W. . 5 Morgan Rhodri M. . 6 Martinez-Lumbreras Santiago . . 7 Isaacson Rivka L. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 135 "13C chemical shifts" 407 "15N chemical shifts" 135 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2017-11-10 original BMRB . stop_ _Original_release_date 2017-03-06 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Structure and Interactions of the TPR Domain of Sgt2 with Yeast Chaperones and Ybr137wp. ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 29075633 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Krysztofinska Ewelina M. . 2 Evans Nicola J. . 3 Thapaliya Arjun . . 4 Murray James W. . 5 Morgan Rhodri M. . 6 Martinez-Lumbreras Santiago . . 7 Isaacson Rivka L. . stop_ _Journal_abbreviation 'Front Mol Biosci' _Journal_name_full 'Frontiers in molecular biosciences' _Journal_volume 4 _Journal_issue 68 _Journal_ISSN 2296-889X _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year 2017 _Details . loop_ _Keyword Chaperone Sgt2 TPR stop_ save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Sgt2_TPR monomer' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Sgt2_TPR monomer' $Sgt2 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_Sgt2 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Sgt2 _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 140 _Mol_residue_sequence ; GSMEDDAETKAKAEDLKMQG NKAMANKDYELAINKYTEAI KVLPTNAIYYANRAAAHSSL KEYDQAVKDAESAISIDPSY FRGYSRLGFAKYAQGKPEEA LEAYKKVLDIEGDNATEAMK RDYESAKKKVEQSLNLEKTV ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 90 GLY 2 91 SER 3 92 MET 4 93 GLU 5 94 ASP 6 95 ASP 7 96 ALA 8 97 GLU 9 98 THR 10 99 LYS 11 100 ALA 12 101 LYS 13 102 ALA 14 103 GLU 15 104 ASP 16 105 LEU 17 106 LYS 18 107 MET 19 108 GLN 20 109 GLY 21 110 ASN 22 111 LYS 23 112 ALA 24 113 MET 25 114 ALA 26 115 ASN 27 116 LYS 28 117 ASP 29 118 TYR 30 119 GLU 31 120 LEU 32 121 ALA 33 122 ILE 34 123 ASN 35 124 LYS 36 125 TYR 37 126 THR 38 127 GLU 39 128 ALA 40 129 ILE 41 130 LYS 42 131 VAL 43 132 LEU 44 133 PRO 45 134 THR 46 135 ASN 47 136 ALA 48 137 ILE 49 138 TYR 50 139 TYR 51 140 ALA 52 141 ASN 53 142 ARG 54 143 ALA 55 144 ALA 56 145 ALA 57 146 HIS 58 147 SER 59 148 SER 60 149 LEU 61 150 LYS 62 151 GLU 63 152 TYR 64 153 ASP 65 154 GLN 66 155 ALA 67 156 VAL 68 157 LYS 69 158 ASP 70 159 ALA 71 160 GLU 72 161 SER 73 162 ALA 74 163 ILE 75 164 SER 76 165 ILE 77 166 ASP 78 167 PRO 79 168 SER 80 169 TYR 81 170 PHE 82 171 ARG 83 172 GLY 84 173 TYR 85 174 SER 86 175 ARG 87 176 LEU 88 177 GLY 89 178 PHE 90 179 ALA 91 180 LYS 92 181 TYR 93 182 ALA 94 183 GLN 95 184 GLY 96 185 LYS 97 186 PRO 98 187 GLU 99 188 GLU 100 189 ALA 101 190 LEU 102 191 GLU 103 192 ALA 104 193 TYR 105 194 LYS 106 195 LYS 107 196 VAL 108 197 LEU 109 198 ASP 110 199 ILE 111 200 GLU 112 201 GLY 113 202 ASP 114 203 ASN 115 204 ALA 116 205 THR 117 206 GLU 118 207 ALA 119 208 MET 120 209 LYS 121 210 ARG 122 211 ASP 123 212 TYR 124 213 GLU 125 214 SER 126 215 ALA 127 216 LYS 128 217 LYS 129 218 LYS 130 219 VAL 131 220 GLU 132 221 GLN 133 222 SER 134 223 LEU 135 224 ASN 136 225 LEU 137 226 GLU 138 227 LYS 139 228 THR 140 229 VAL stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $Sgt2 "baker's yeast" 4932 Eukaryota Fungi Saccharomyces cerevisiae stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $Sgt2 'recombinant technology' . Escherichia coli . Pet28 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Sgt2 0.5 mM '[U-13C; U-15N]' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 100 mM 'natural abundance' TCEP 0.5 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection 'data analysis' stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_CCPN _Saveframe_category software _Name CCPN _Version . loop_ _Vendor _Address _Electronic_address CCPN . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' 'peak picking' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 500 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCACB_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ save_3D_HNCO_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCO' _Sample_label $sample_1 save_ save_3D_HN(CO)CA_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HN(CO)CA' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_3D_CBCA(CO)NH_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D CBCA(CO)NH' _Sample_label $sample_1 save_ save_3D_HNCACB_8 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HNCACB' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.010 . M pH 6.0 . pH pressure 1 . atm temperature 293 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CCPN stop_ loop_ _Experiment_label '2D 1H-15N HSQC' '3D CBCA(CO)NH' '3D HNCACB' '3D HNCO' '3D HN(CO)CA' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'Sgt2_TPR monomer' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 90 1 GLY CA C 43.588 0.016 1 2 91 2 SER H H 8.855 0.003 1 3 91 2 SER C C 175.344 0.000 1 4 91 2 SER CA C 59.511 0.024 1 5 91 2 SER CB C 63.664 0.017 1 6 91 2 SER N N 117.333 0.057 1 7 92 3 MET H H 8.971 0.005 1 8 92 3 MET C C 176.827 0.005 1 9 92 3 MET CA C 57.581 0.007 1 10 92 3 MET CB C 29.402 0.030 1 11 92 3 MET N N 123.155 0.006 1 12 93 4 GLU H H 8.176 0.002 1 13 93 4 GLU CA C 54.993 0.027 1 14 93 4 GLU CB C 41.206 0.000 1 15 93 4 GLU N N 121.083 0.005 1 16 94 5 ASP H H 8.266 0.003 1 17 94 5 ASP CA C 55.534 0.044 1 18 94 5 ASP CB C 41.081 0.000 1 19 94 5 ASP N N 122.089 0.001 1 20 95 6 ASP H H 8.269 0.003 1 21 95 6 ASP C C 177.395 0.003 1 22 95 6 ASP CA C 55.571 0.007 1 23 95 6 ASP CB C 41.101 0.011 1 24 95 6 ASP N N 122.087 0.004 1 25 96 7 ALA H H 8.396 0.003 1 26 96 7 ALA C C 180.690 0.000 1 27 96 7 ALA CA C 55.425 0.032 1 28 96 7 ALA CB C 18.486 0.038 1 29 96 7 ALA N N 125.006 0.005 1 30 97 8 GLU H H 8.493 0.004 1 31 97 8 GLU C C 179.232 0.010 1 32 97 8 GLU CA C 59.482 0.103 1 33 97 8 GLU CB C 29.349 0.025 1 34 97 8 GLU N N 120.439 0.005 1 35 98 9 THR H H 8.144 0.002 1 36 98 9 THR C C 175.704 0.009 1 37 98 9 THR CA C 66.792 0.007 1 38 98 9 THR CB C 68.706 0.013 1 39 98 9 THR N N 119.383 0.002 1 40 99 10 LYS H H 8.042 0.004 1 41 99 10 LYS C C 178.043 0.006 1 42 99 10 LYS CA C 60.174 0.092 1 43 99 10 LYS CB C 32.121 0.014 1 44 99 10 LYS N N 121.047 0.002 1 45 100 11 ALA H H 8.006 0.003 1 46 100 11 ALA C C 180.852 0.009 1 47 100 11 ALA CA C 55.234 0.065 1 48 100 11 ALA CB C 17.950 0.008 1 49 100 11 ALA N N 120.714 0.013 1 50 101 12 LYS H H 7.965 0.003 1 51 101 12 LYS C C 179.267 0.000 1 52 101 12 LYS CA C 59.360 0.009 1 53 101 12 LYS CB C 32.498 0.007 1 54 101 12 LYS N N 120.726 0.007 1 55 102 13 ALA H H 8.241 0.003 1 56 102 13 ALA C C 179.245 0.022 1 57 102 13 ALA CA C 55.933 0.010 1 58 102 13 ALA CB C 19.374 0.025 1 59 102 13 ALA N N 123.625 0.024 1 60 103 14 GLU H H 8.719 0.003 1 61 103 14 GLU C C 179.176 0.000 1 62 103 14 GLU CA C 58.615 0.090 1 63 103 14 GLU CB C 30.286 0.024 1 64 103 14 GLU N N 117.164 0.107 1 65 104 15 ASP H H 7.943 0.003 1 66 104 15 ASP C C 178.798 0.000 1 67 104 15 ASP CA C 57.689 0.012 1 68 104 15 ASP CB C 41.599 0.006 1 69 104 15 ASP N N 122.012 0.003 1 70 105 16 LEU H H 7.555 0.003 1 71 105 16 LEU C C 179.007 0.008 1 72 105 16 LEU CA C 58.556 0.016 1 73 105 16 LEU CB C 41.622 0.000 1 74 105 16 LEU N N 122.195 0.000 1 75 106 17 LYS H H 8.558 0.004 1 76 106 17 LYS C C 178.521 0.016 1 77 106 17 LYS CA C 60.114 0.025 1 78 106 17 LYS CB C 30.057 0.025 1 79 106 17 LYS N N 121.370 0.018 1 80 107 18 MET H H 8.565 0.004 1 81 107 18 MET C C 179.701 0.001 1 82 107 18 MET CA C 58.967 0.008 1 83 107 18 MET CB C 31.178 0.048 1 84 107 18 MET N N 120.148 0.005 1 85 108 19 GLN H H 8.383 0.003 1 86 108 19 GLN C C 180.527 0.000 1 87 108 19 GLN CA C 59.607 0.005 1 88 108 19 GLN CB C 29.080 0.000 1 89 108 19 GLN N N 121.004 0.010 1 90 109 20 GLY H H 8.612 0.002 1 91 109 20 GLY C C 174.104 0.006 1 92 109 20 GLY CA C 47.576 0.036 1 93 109 20 GLY N N 111.221 0.017 1 94 110 21 ASN H H 9.085 0.002 1 95 110 21 ASN C C 178.851 0.000 1 96 110 21 ASN CA C 55.614 0.009 1 97 110 21 ASN CB C 36.805 0.028 1 98 110 21 ASN N N 122.916 0.001 1 99 111 22 LYS H H 8.361 0.002 1 100 111 22 LYS C C 178.345 0.003 1 101 111 22 LYS CA C 59.497 0.003 1 102 111 22 LYS CB C 32.024 0.032 1 103 111 22 LYS N N 124.554 0.009 1 104 112 23 ALA H H 7.626 0.002 1 105 112 23 ALA C C 180.370 0.001 1 106 112 23 ALA CA C 55.322 0.016 1 107 112 23 ALA CB C 16.911 0.034 1 108 112 23 ALA N N 123.140 0.009 1 109 113 24 MET H H 8.460 0.003 1 110 113 24 MET C C 180.821 0.003 1 111 113 24 MET CA C 58.002 0.050 1 112 113 24 MET CB C 31.168 0.013 1 113 113 24 MET N N 118.588 0.004 1 114 114 25 ALA H H 7.904 0.002 1 115 114 25 ALA C C 178.683 0.000 1 116 114 25 ALA CA C 54.896 0.012 1 117 114 25 ALA CB C 17.750 0.029 1 118 114 25 ALA N N 124.673 0.003 1 119 115 26 ASN H H 7.495 0.002 1 120 115 26 ASN C C 173.989 0.007 1 121 115 26 ASN CA C 52.917 0.080 1 122 115 26 ASN CB C 39.082 0.000 1 123 115 26 ASN N N 116.316 0.007 1 124 116 27 LYS H H 7.814 0.002 1 125 116 27 LYS C C 173.902 0.010 1 126 116 27 LYS CA C 57.387 0.052 1 127 116 27 LYS CB C 28.678 0.021 1 128 116 27 LYS N N 115.163 0.022 1 129 117 28 ASP H H 8.102 0.002 1 130 117 28 ASP C C 176.304 0.005 1 131 117 28 ASP CA C 51.113 0.017 1 132 117 28 ASP CB C 39.292 0.043 1 133 117 28 ASP N N 120.675 0.011 1 134 118 29 TYR H H 6.976 0.003 1 135 118 29 TYR C C 177.797 0.007 1 136 118 29 TYR CA C 60.877 0.016 1 137 118 29 TYR CB C 37.628 0.005 1 138 118 29 TYR N N 121.195 0.022 1 139 119 30 GLU H H 8.932 0.005 1 140 119 30 GLU C C 179.032 0.001 1 141 119 30 GLU CA C 59.900 0.019 1 142 119 30 GLU CB C 28.989 0.026 1 143 119 30 GLU N N 119.165 0.012 1 144 120 31 LEU H H 7.364 0.003 1 145 120 31 LEU C C 178.610 0.003 1 146 120 31 LEU CA C 57.456 0.028 1 147 120 31 LEU CB C 41.468 0.026 1 148 120 31 LEU N N 122.343 0.004 1 149 121 32 ALA H H 7.919 0.004 1 150 121 32 ALA C C 178.177 0.011 1 151 121 32 ALA CA C 56.084 0.116 1 152 121 32 ALA CB C 18.263 0.084 1 153 121 32 ALA N N 121.319 0.008 1 154 122 33 ILE H H 8.292 0.005 1 155 122 33 ILE C C 179.576 0.004 1 156 122 33 ILE CA C 66.780 0.042 1 157 122 33 ILE CB C 37.519 0.005 1 158 122 33 ILE N N 117.404 0.019 1 159 123 34 ASN H H 7.924 0.004 1 160 123 34 ASN C C 177.730 0.012 1 161 123 34 ASN CA C 57.174 0.053 1 162 123 34 ASN CB C 38.394 0.027 1 163 123 34 ASN N N 123.306 0.003 1 164 124 35 LYS H H 8.453 0.002 1 165 124 35 LYS C C 179.290 0.025 1 166 124 35 LYS CA C 57.518 0.037 1 167 124 35 LYS CB C 30.364 0.001 1 168 124 35 LYS N N 120.498 0.001 1 169 125 36 TYR H H 8.842 0.008 1 170 125 36 TYR C C 178.112 0.002 1 171 125 36 TYR CA C 59.007 0.034 1 172 125 36 TYR CB C 37.463 0.005 1 173 125 36 TYR N N 117.270 0.037 1 174 126 37 THR H H 8.291 0.003 1 175 126 37 THR C C 175.676 0.000 1 176 126 37 THR CA C 67.487 0.015 1 177 126 37 THR CB C 68.494 0.065 1 178 126 37 THR N N 116.370 0.002 1 179 127 38 GLU H H 7.943 0.002 1 180 127 38 GLU C C 178.360 0.003 1 181 127 38 GLU CA C 59.614 0.003 1 182 127 38 GLU CB C 29.600 0.009 1 183 127 38 GLU N N 123.483 0.003 1 184 128 39 ALA H H 8.060 0.004 1 185 128 39 ALA C C 178.208 0.010 1 186 128 39 ALA CA C 56.029 0.005 1 187 128 39 ALA CB C 16.460 0.005 1 188 128 39 ALA N N 123.767 0.003 1 189 129 40 ILE H H 7.848 0.003 1 190 129 40 ILE C C 176.496 0.002 1 191 129 40 ILE CA C 64.715 0.017 1 192 129 40 ILE CB C 39.040 0.053 1 193 129 40 ILE N N 119.266 0.002 1 194 130 41 LYS H H 7.656 0.003 1 195 130 41 LYS C C 179.312 0.001 1 196 130 41 LYS CA C 58.971 0.032 1 197 130 41 LYS CB C 32.341 0.050 1 198 130 41 LYS N N 117.817 0.001 1 199 131 42 VAL H H 7.471 0.004 1 200 131 42 VAL C C 175.783 0.005 1 201 131 42 VAL CA C 65.145 0.006 1 202 131 42 VAL CB C 32.200 0.017 1 203 131 42 VAL N N 118.217 0.002 1 204 132 43 LEU H H 8.491 0.005 1 205 132 43 LEU C C 179.245 0.000 1 206 132 43 LEU CA C 52.348 0.000 1 207 132 43 LEU CB C 44.648 0.000 1 208 132 43 LEU N N 121.462 0.062 1 209 133 44 PRO C C 178.660 0.000 1 210 133 44 PRO CA C 65.003 0.000 1 211 133 44 PRO CB C 32.160 0.000 1 212 134 45 THR H H 7.150 0.003 1 213 134 45 THR C C 172.349 0.000 1 214 134 45 THR CA C 60.532 0.032 1 215 134 45 THR CB C 67.965 0.031 1 216 134 45 THR N N 108.232 0.015 1 217 135 46 ASN H H 7.864 0.003 1 218 135 46 ASN C C 175.453 0.010 1 219 135 46 ASN CA C 51.881 0.002 1 220 135 46 ASN CB C 39.250 0.000 1 221 135 46 ASN N N 120.722 0.011 1 222 136 47 ALA H H 9.128 0.005 1 223 136 47 ALA C C 178.608 0.002 1 224 136 47 ALA CA C 55.573 0.023 1 225 136 47 ALA CB C 20.243 0.051 1 226 136 47 ALA N N 128.579 0.047 1 227 137 48 ILE H H 7.685 0.004 1 228 137 48 ILE C C 178.357 0.020 1 229 137 48 ILE CA C 64.966 0.078 1 230 137 48 ILE CB C 37.379 0.073 1 231 137 48 ILE N N 116.319 0.005 1 232 138 49 TYR H H 6.837 0.002 1 233 138 49 TYR C C 178.401 0.003 1 234 138 49 TYR CA C 57.931 0.008 1 235 138 49 TYR CB C 36.856 0.001 1 236 138 49 TYR N N 117.627 0.047 1 237 139 50 TYR H H 6.881 0.003 1 238 139 50 TYR C C 178.068 0.013 1 239 139 50 TYR CA C 62.426 0.020 1 240 139 50 TYR CB C 38.566 0.094 1 241 139 50 TYR N N 116.222 0.004 1 242 140 51 ALA H H 8.104 0.006 1 243 140 51 ALA C C 179.407 0.000 1 244 140 51 ALA CA C 55.481 0.067 1 245 140 51 ALA CB C 17.833 0.021 1 246 140 51 ALA N N 122.167 0.003 1 247 141 52 ASN H H 8.479 0.007 1 248 141 52 ASN C C 177.076 0.008 1 249 141 52 ASN CA C 55.197 0.024 1 250 141 52 ASN CB C 34.999 0.067 1 251 141 52 ASN N N 119.329 0.001 1 252 142 53 ARG H H 7.911 0.003 1 253 142 53 ARG C C 178.159 0.001 1 254 142 53 ARG CA C 61.141 0.035 1 255 142 53 ARG CB C 30.256 0.006 1 256 142 53 ARG N N 124.052 0.005 1 257 143 54 ALA H H 8.761 0.002 1 258 143 54 ALA C C 179.573 0.010 1 259 143 54 ALA CA C 56.075 0.037 1 260 143 54 ALA CB C 18.053 0.043 1 261 143 54 ALA N N 122.374 0.006 1 262 144 55 ALA H H 7.490 0.002 1 263 144 55 ALA C C 180.357 0.005 1 264 144 55 ALA CA C 55.007 0.038 1 265 144 55 ALA CB C 17.540 0.058 1 266 144 55 ALA N N 120.156 0.005 1 267 145 56 ALA H H 7.768 0.002 1 268 145 56 ALA C C 178.830 0.009 1 269 145 56 ALA CA C 56.019 0.000 1 270 145 56 ALA CB C 19.339 0.000 1 271 145 56 ALA N N 123.200 0.008 1 272 146 57 HIS H H 8.625 0.007 1 273 146 57 HIS C C 179.143 0.000 1 274 146 57 HIS CA C 60.302 0.007 1 275 146 57 HIS CB C 27.777 0.009 1 276 146 57 HIS N N 114.977 0.009 1 277 147 58 SER H H 9.152 0.009 1 278 147 58 SER CA C 64.120 0.033 1 279 147 58 SER CB C 63.076 0.028 1 280 147 58 SER N N 120.126 0.103 1 281 148 59 SER H H 7.979 0.003 1 282 148 59 SER C C 174.486 0.000 1 283 148 59 SER CA C 62.868 0.046 1 284 148 59 SER CB C 61.490 0.039 1 285 148 59 SER N N 122.045 0.009 1 286 149 60 LEU H H 6.920 0.003 1 287 149 60 LEU C C 175.564 0.011 1 288 149 60 LEU CA C 54.349 0.036 1 289 149 60 LEU CB C 42.638 0.012 1 290 149 60 LEU N N 121.002 0.003 1 291 150 61 LYS H H 7.750 0.004 1 292 150 61 LYS C C 175.495 0.005 1 293 150 61 LYS CA C 57.426 0.002 1 294 150 61 LYS CB C 28.521 0.031 1 295 150 61 LYS N N 114.365 0.010 1 296 151 62 GLU H H 7.970 0.016 1 297 151 62 GLU C C 178.177 0.003 1 298 151 62 GLU CA C 53.199 0.041 1 299 151 62 GLU CB C 27.029 0.017 1 300 151 62 GLU N N 121.851 0.006 1 301 152 63 TYR H H 7.156 0.004 1 302 152 63 TYR C C 177.420 0.001 1 303 152 63 TYR CA C 61.224 0.046 1 304 152 63 TYR CB C 38.007 0.012 1 305 152 63 TYR N N 119.718 0.065 1 306 153 64 ASP H H 8.661 0.006 1 307 153 64 ASP C C 179.483 0.009 1 308 153 64 ASP CA C 57.780 0.019 1 309 153 64 ASP CB C 39.528 0.046 1 310 153 64 ASP N N 119.505 0.086 1 311 154 65 GLN H H 8.079 0.003 1 312 154 65 GLN C C 177.951 0.018 1 313 154 65 GLN CA C 58.674 0.030 1 314 154 65 GLN CB C 27.103 0.025 1 315 154 65 GLN N N 120.771 0.009 1 316 155 66 ALA H H 8.001 0.007 1 317 155 66 ALA C C 179.044 0.008 1 318 155 66 ALA CA C 56.279 0.004 1 319 155 66 ALA CB C 20.566 0.009 1 320 155 66 ALA N N 122.826 0.005 1 321 156 67 VAL H H 8.438 0.002 1 322 156 67 VAL C C 177.121 0.008 1 323 156 67 VAL CA C 67.615 0.015 1 324 156 67 VAL CB C 31.700 0.003 1 325 156 67 VAL N N 118.257 0.006 1 326 157 68 LYS H H 7.284 0.002 1 327 157 68 LYS C C 179.580 0.010 1 328 157 68 LYS CA C 59.495 0.046 1 329 157 68 LYS CB C 32.055 0.033 1 330 157 68 LYS N N 118.536 0.005 1 331 158 69 ASP H H 8.024 0.010 1 332 158 69 ASP C C 178.357 0.002 1 333 158 69 ASP CA C 57.990 0.028 1 334 158 69 ASP CB C 40.245 0.012 1 335 158 69 ASP N N 121.053 0.013 1 336 159 70 ALA H H 8.891 0.003 1 337 159 70 ALA C C 179.181 0.001 1 338 159 70 ALA CA C 55.738 0.011 1 339 159 70 ALA CB C 18.345 0.010 1 340 159 70 ALA N N 125.273 0.002 1 341 160 71 GLU H H 8.846 0.004 1 342 160 71 GLU C C 180.891 0.002 1 343 160 71 GLU CA C 59.868 0.013 1 344 160 71 GLU CB C 28.752 0.054 1 345 160 71 GLU N N 116.549 0.002 1 346 161 72 SER H H 8.342 0.004 1 347 161 72 SER C C 175.529 0.000 1 348 161 72 SER CA C 62.686 0.006 1 349 161 72 SER N N 119.874 0.008 1 350 162 73 ALA H H 7.880 0.008 1 351 162 73 ALA C C 178.635 0.003 1 352 162 73 ALA CA C 55.953 0.013 1 353 162 73 ALA CB C 17.788 0.007 1 354 162 73 ALA N N 124.933 0.010 1 355 163 74 ILE H H 8.006 0.004 1 356 163 74 ILE C C 177.049 0.008 1 357 163 74 ILE CA C 64.622 0.023 1 358 163 74 ILE CB C 39.141 0.054 1 359 163 74 ILE N N 119.270 0.011 1 360 164 75 SER H H 7.719 0.002 1 361 164 75 SER C C 176.563 0.000 1 362 164 75 SER CA C 60.865 0.015 1 363 164 75 SER CB C 63.253 0.007 1 364 164 75 SER N N 113.594 0.103 1 365 165 76 ILE H H 7.662 0.004 1 366 165 76 ILE C C 176.925 0.002 1 367 165 76 ILE CA C 64.210 0.041 1 368 165 76 ILE CB C 39.032 0.016 1 369 165 76 ILE N N 121.833 0.004 1 370 166 77 ASP H H 8.190 0.003 1 371 166 77 ASP CA C 50.920 0.000 1 372 166 77 ASP CB C 41.782 0.000 1 373 166 77 ASP N N 115.459 0.003 1 374 167 78 PRO C C 178.497 0.000 1 375 167 78 PRO CA C 63.725 0.000 1 376 167 78 PRO CB C 32.054 0.051 1 377 168 79 SER H H 8.425 0.002 1 378 168 79 SER C C 173.377 0.000 1 379 168 79 SER CA C 58.642 0.033 1 380 168 79 SER CB C 64.317 0.042 1 381 168 79 SER N N 116.264 0.006 1 382 169 80 TYR H H 7.721 0.005 1 383 169 80 TYR C C 177.973 0.005 1 384 169 80 TYR CA C 55.754 0.023 1 385 169 80 TYR CB C 36.598 0.040 1 386 169 80 TYR N N 124.920 0.003 1 387 170 81 PHE H H 8.969 0.026 1 388 170 81 PHE C C 176.564 0.000 1 389 170 81 PHE CA C 61.030 0.000 1 390 170 81 PHE CB C 38.055 0.000 1 391 170 81 PHE N N 128.445 0.008 1 392 171 82 ARG C C 178.258 0.000 1 393 171 82 ARG CA C 57.994 0.045 1 394 171 82 ARG CB C 29.654 0.014 1 395 172 83 GLY H H 6.933 0.002 1 396 172 83 GLY C C 174.409 0.000 1 397 172 83 GLY CA C 47.905 0.026 1 398 172 83 GLY N N 105.351 0.004 1 399 173 84 TYR H H 6.622 0.007 1 400 173 84 TYR C C 178.514 0.016 1 401 173 84 TYR CA C 61.423 0.032 1 402 173 84 TYR CB C 37.825 0.020 1 403 173 84 TYR N N 118.856 0.007 1 404 174 85 SER H H 7.947 0.012 1 405 174 85 SER C C 177.142 0.000 1 406 174 85 SER CA C 63.018 0.057 1 407 174 85 SER N N 115.812 0.004 1 408 175 86 ARG H H 8.077 0.011 1 409 175 86 ARG C C 179.367 0.013 1 410 175 86 ARG CA C 57.786 0.010 1 411 175 86 ARG CB C 29.186 0.040 1 412 175 86 ARG N N 118.868 0.180 1 413 176 87 LEU H H 7.908 0.004 1 414 176 87 LEU C C 177.810 0.001 1 415 176 87 LEU CA C 58.210 0.025 1 416 176 87 LEU CB C 42.368 0.068 1 417 176 87 LEU N N 122.827 0.000 1 418 177 88 GLY H H 8.684 0.006 1 419 177 88 GLY C C 174.275 0.009 1 420 177 88 GLY CA C 47.804 0.017 1 421 177 88 GLY N N 106.010 0.010 1 422 178 89 PHE H H 8.043 0.004 1 423 178 89 PHE C C 178.118 0.016 1 424 178 89 PHE CA C 62.417 0.012 1 425 178 89 PHE CB C 39.653 0.041 1 426 178 89 PHE N N 122.403 0.063 1 427 179 90 ALA H H 7.594 0.004 1 428 179 90 ALA C C 178.654 0.013 1 429 179 90 ALA CA C 55.266 0.003 1 430 179 90 ALA CB C 19.236 0.014 1 431 179 90 ALA N N 121.620 0.011 1 432 180 91 LYS H H 8.379 0.005 1 433 180 91 LYS C C 179.047 0.006 1 434 180 91 LYS CA C 57.154 0.007 1 435 180 91 LYS CB C 31.693 0.010 1 436 180 91 LYS N N 115.412 0.020 1 437 181 92 TYR H H 8.593 0.004 1 438 181 92 TYR C C 179.866 0.003 1 439 181 92 TYR CA C 62.514 0.009 1 440 181 92 TYR CB C 37.803 0.070 1 441 181 92 TYR N N 121.388 0.013 1 442 182 93 ALA H H 7.932 0.004 1 443 182 93 ALA C C 179.256 0.003 1 444 182 93 ALA CA C 54.517 0.009 1 445 182 93 ALA CB C 16.528 0.042 1 446 182 93 ALA N N 124.397 0.011 1 447 183 94 GLN H H 7.351 0.003 1 448 183 94 GLN C C 176.195 0.008 1 449 183 94 GLN CA C 56.035 0.019 1 450 183 94 GLN CB C 30.287 0.011 1 451 183 94 GLN N N 115.077 0.055 1 452 184 95 GLY H H 7.731 0.002 1 453 184 95 GLY C C 174.151 0.000 1 454 184 95 GLY CA C 45.928 0.004 1 455 184 95 GLY N N 107.932 0.059 1 456 185 96 LYS H H 7.807 0.006 1 457 185 96 LYS C C 174.616 0.000 1 458 185 96 LYS CA C 53.583 0.000 1 459 185 96 LYS CB C 31.899 0.000 1 460 185 96 LYS N N 119.142 0.004 1 461 186 97 PRO C C 177.409 0.004 1 462 186 97 PRO CA C 65.586 0.000 1 463 186 97 PRO CB C 31.144 0.011 1 464 187 98 GLU H H 9.219 0.026 1 465 187 98 GLU C C 179.467 0.004 1 466 187 98 GLU CA C 60.570 0.024 1 467 187 98 GLU CB C 28.071 0.040 1 468 187 98 GLU N N 121.876 0.016 1 469 188 99 GLU H H 7.453 0.003 1 470 188 99 GLU C C 180.036 0.006 1 471 188 99 GLU CA C 59.336 0.008 1 472 188 99 GLU CB C 30.009 0.011 1 473 188 99 GLU N N 119.913 0.012 1 474 189 100 ALA H H 7.971 0.004 1 475 189 100 ALA C C 177.517 0.005 1 476 189 100 ALA CA C 55.373 0.031 1 477 189 100 ALA CB C 18.739 0.000 1 478 189 100 ALA N N 123.574 0.071 1 479 190 101 LEU H H 8.617 0.004 1 480 190 101 LEU C C 177.931 0.008 1 481 190 101 LEU CA C 58.961 0.035 1 482 190 101 LEU CB C 42.175 0.065 1 483 190 101 LEU N N 118.819 0.005 1 484 191 102 GLU H H 7.283 0.014 1 485 191 102 GLU C C 179.113 0.006 1 486 191 102 GLU CA C 59.245 0.043 1 487 191 102 GLU CB C 29.461 0.036 1 488 191 102 GLU N N 116.592 0.002 1 489 192 103 ALA H H 7.352 0.003 1 490 192 103 ALA C C 179.442 0.000 1 491 192 103 ALA CA C 55.614 0.024 1 492 192 103 ALA CB C 17.435 0.049 1 493 192 103 ALA N N 123.415 0.009 1 494 193 104 TYR H H 8.883 0.003 1 495 193 104 TYR C C 180.154 0.002 1 496 193 104 TYR CA C 58.772 0.021 1 497 193 104 TYR CB C 37.408 0.006 1 498 193 104 TYR N N 116.219 0.003 1 499 194 105 LYS H H 9.095 0.004 1 500 194 105 LYS C C 177.869 0.002 1 501 194 105 LYS CA C 59.251 0.054 1 502 194 105 LYS CB C 32.344 0.029 1 503 194 105 LYS N N 123.522 0.036 1 504 195 106 LYS H H 7.504 0.003 1 505 195 106 LYS C C 178.674 0.003 1 506 195 106 LYS CA C 58.324 0.011 1 507 195 106 LYS CB C 30.772 0.030 1 508 195 106 LYS N N 120.732 0.005 1 509 196 107 VAL H H 7.365 0.006 1 510 196 107 VAL C C 177.342 0.008 1 511 196 107 VAL CA C 67.955 0.008 1 512 196 107 VAL CB C 31.838 0.012 1 513 196 107 VAL N N 117.031 0.003 1 514 197 108 LEU H H 7.003 0.010 1 515 197 108 LEU C C 180.409 0.004 1 516 197 108 LEU CA C 57.942 0.043 1 517 197 108 LEU CB C 40.149 0.025 1 518 197 108 LEU N N 117.084 0.008 1 519 198 109 ASP H H 8.520 0.008 1 520 198 109 ASP C C 178.781 0.005 1 521 198 109 ASP CA C 57.115 0.000 1 522 198 109 ASP CB C 40.359 0.023 1 523 198 109 ASP N N 122.734 0.002 1 524 199 110 ILE H H 8.217 0.002 1 525 199 110 ILE C C 178.561 0.000 1 526 199 110 ILE CA C 65.016 0.000 1 527 199 110 ILE CB C 39.364 0.038 1 528 199 110 ILE N N 120.778 0.003 1 529 200 111 GLU H H 8.365 0.004 1 530 200 111 GLU C C 178.983 0.000 1 531 200 111 GLU CA C 57.047 0.043 1 532 200 111 GLU CB C 30.533 0.083 1 533 200 111 GLU N N 116.814 0.008 1 534 201 112 GLY H H 8.272 0.009 1 535 201 112 GLY C C 177.125 0.012 1 536 201 112 GLY CA C 47.052 0.006 1 537 201 112 GLY N N 110.593 0.122 1 538 202 113 ASP H H 9.181 0.003 1 539 202 113 ASP C C 176.412 0.000 1 540 202 113 ASP CA C 56.167 0.010 1 541 202 113 ASP CB C 40.180 0.021 1 542 202 113 ASP N N 128.377 0.015 1 543 203 114 ASN H H 8.186 0.008 1 544 203 114 ASN C C 175.848 0.021 1 545 203 114 ASN CA C 53.015 0.073 1 546 203 114 ASN CB C 38.880 0.053 1 547 203 114 ASN N N 115.930 0.050 1 548 204 115 ALA H H 7.557 0.015 1 549 204 115 ALA C C 177.436 0.008 1 550 204 115 ALA CA C 52.899 0.040 1 551 204 115 ALA CB C 19.106 0.073 1 552 204 115 ALA N N 124.057 0.031 1 553 205 116 THR H H 8.199 0.016 1 554 205 116 THR C C 175.634 0.018 1 555 205 116 THR CA C 60.074 0.025 1 556 205 116 THR CB C 71.518 0.000 1 557 205 116 THR N N 115.127 0.033 1 558 206 117 GLU H H 8.903 0.004 1 559 206 117 GLU C C 178.980 0.008 1 560 206 117 GLU CA C 59.643 0.038 1 561 206 117 GLU CB C 28.919 0.000 1 562 206 117 GLU N N 121.078 0.005 1 563 207 118 ALA H H 8.231 0.002 1 564 207 118 ALA C C 179.309 0.000 1 565 207 118 ALA CA C 55.384 0.083 1 566 207 118 ALA CB C 18.346 0.035 1 567 207 118 ALA N N 121.619 0.083 1 568 208 119 MET H H 7.366 0.003 1 569 208 119 MET C C 180.432 0.096 1 570 208 119 MET CA C 58.901 0.005 1 571 208 119 MET CB C 32.116 0.060 1 572 208 119 MET N N 117.581 0.043 1 573 209 120 LYS H H 7.759 0.002 1 574 209 120 LYS C C 178.361 0.017 1 575 209 120 LYS CA C 60.337 0.032 1 576 209 120 LYS CB C 32.050 0.000 1 577 209 120 LYS N N 121.887 0.023 1 578 210 121 ARG H H 8.509 0.003 1 579 210 121 ARG C C 179.380 0.000 1 580 210 121 ARG CA C 59.375 0.003 1 581 210 121 ARG CB C 29.861 0.039 1 582 210 121 ARG N N 121.624 0.039 1 583 211 122 ASP H H 8.041 0.003 1 584 211 122 ASP C C 177.705 0.002 1 585 211 122 ASP CA C 57.557 0.014 1 586 211 122 ASP CB C 38.492 0.003 1 587 211 122 ASP N N 122.369 0.010 1 588 212 123 TYR H H 6.879 0.004 1 589 212 123 TYR C C 175.871 0.010 1 590 212 123 TYR CA C 61.900 0.003 1 591 212 123 TYR CB C 39.194 0.003 1 592 212 123 TYR N N 118.869 0.008 1 593 213 124 GLU H H 8.496 0.002 1 594 213 124 GLU C C 179.474 0.015 1 595 213 124 GLU CA C 59.294 0.021 1 596 213 124 GLU CB C 29.368 0.026 1 597 213 124 GLU N N 119.153 0.005 1 598 214 125 SER H H 8.608 0.002 1 599 214 125 SER C C 177.274 0.000 1 600 214 125 SER CA C 61.519 0.009 1 601 214 125 SER CB C 62.354 0.026 1 602 214 125 SER N N 116.250 0.008 1 603 215 126 ALA H H 7.619 0.003 1 604 215 126 ALA C C 177.913 0.005 1 605 215 126 ALA CA C 55.662 0.034 1 606 215 126 ALA CB C 17.676 0.056 1 607 215 126 ALA N N 125.641 0.006 1 608 216 127 LYS H H 8.385 0.003 1 609 216 127 LYS C C 178.479 0.005 1 610 216 127 LYS CA C 59.995 0.014 1 611 216 127 LYS CB C 32.528 0.021 1 612 216 127 LYS N N 120.580 0.009 1 613 217 128 LYS H H 8.144 0.001 1 614 217 128 LYS C C 178.835 0.004 1 615 217 128 LYS CA C 58.876 0.035 1 616 217 128 LYS CB C 32.302 0.004 1 617 217 128 LYS N N 118.764 0.014 1 618 218 129 LYS H H 7.558 0.003 1 619 218 129 LYS C C 179.566 0.008 1 620 218 129 LYS CA C 58.004 0.006 1 621 218 129 LYS CB C 32.183 0.002 1 622 218 129 LYS N N 119.419 0.002 1 623 219 130 VAL H H 8.153 0.003 1 624 219 130 VAL C C 178.622 0.018 1 625 219 130 VAL CA C 66.638 0.019 1 626 219 130 VAL CB C 31.835 0.040 1 627 219 130 VAL N N 121.299 0.002 1 628 220 131 GLU H H 8.264 0.003 1 629 220 131 GLU C C 178.749 0.006 1 630 220 131 GLU CA C 59.123 0.007 1 631 220 131 GLU CB C 29.470 0.018 1 632 220 131 GLU N N 120.434 0.005 1 633 221 132 GLN H H 8.076 0.002 1 634 221 132 GLN C C 177.640 0.005 1 635 221 132 GLN CA C 57.766 0.020 1 636 221 132 GLN CB C 28.758 0.020 1 637 221 132 GLN N N 118.606 0.000 1 638 222 133 SER H H 7.903 0.002 1 639 222 133 SER C C 175.039 0.000 1 640 222 133 SER CA C 59.897 0.038 1 641 222 133 SER CB C 63.665 0.029 1 642 222 133 SER N N 115.714 0.002 1 643 223 134 LEU H H 7.789 0.003 1 644 223 134 LEU C C 177.414 0.003 1 645 223 134 LEU CA C 55.840 0.012 1 646 223 134 LEU CB C 42.355 0.033 1 647 223 134 LEU N N 122.931 0.090 1 648 224 135 ASN H H 8.128 0.002 1 649 224 135 ASN C C 175.315 0.006 1 650 224 135 ASN CA C 53.565 0.007 1 651 224 135 ASN CB C 38.633 0.021 1 652 224 135 ASN N N 118.753 0.002 1 653 225 136 LEU H H 8.093 0.005 1 654 225 136 LEU C C 177.687 0.004 1 655 225 136 LEU CA C 55.566 0.004 1 656 225 136 LEU CB C 42.320 0.007 1 657 225 136 LEU N N 122.448 0.154 1 658 226 137 GLU H H 8.315 0.007 1 659 226 137 GLU C C 176.408 0.008 1 660 226 137 GLU CA C 56.728 0.022 1 661 226 137 GLU CB C 30.008 0.002 1 662 226 137 GLU N N 121.622 0.001 1 663 227 138 LYS H H 8.229 0.005 1 664 227 138 LYS C C 176.644 0.012 1 665 227 138 LYS CA C 56.157 0.003 1 666 227 138 LYS CB C 33.146 0.019 1 667 227 138 LYS N N 122.989 0.026 1 668 228 139 THR H H 8.288 0.005 1 669 228 139 THR C C 173.894 0.003 1 670 228 139 THR CA C 62.096 0.019 1 671 228 139 THR CB C 69.918 0.013 1 672 228 139 THR N N 117.449 0.116 1 673 229 140 VAL H H 7.778 0.002 1 674 229 140 VAL C C 180.995 0.000 1 675 229 140 VAL CA C 63.716 0.000 1 676 229 140 VAL CB C 33.246 0.000 1 677 229 140 VAL N N 127.008 0.006 1 stop_ save_