data_26636 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 1H and 15N Chemical Shifts of Intrinsically Disordered Dehydrin ERD14 at Various Temperature and pH Values ; _BMRB_accession_number 26636 _BMRB_flat_file_name bmr26636.str _Entry_type original _Submission_date 2015-08-15 _Accession_date 2015-08-15 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'Chemical shift assignments of BMRB entry 16876 have been transferred to phosphate buffer and several different T and pH values.' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 'Szalaine Agoston' Bianka . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 8 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 916 "15N chemical shifts" 916 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2015-09-15 original BMRB . stop_ loop_ _Related_BMRB_accession_number _Relationship 16876 ; Entry containing full assignment for the same protein using MES buffer and one specific condition of T/pH. It is the basis of the present assignments. ; stop_ _Original_release_date 2015-09-15 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Interplay of structural disorder and induced folding in the chaperone activity of intrinsically disordered plant dehydrin ERD14 in vivo ; _Citation_status 'in preparation' _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Kalmar Lajos . . 2 'Szalaine Agoston' Bianka . . 3 Magos Zoltan . . 4 Felmery Anna . . 5 Szabo Beata . . 6 Tantos Agnes . . 7 Murvai Nikoletta . . 8 Csikos Gyorgy . . 9 Vertomen Didier . . 10 Hristova Nevena . . 11 Kovacs Denes . . 12 Lang Andras . . 13 Buday Laszlo . . 14 Han Kyou-Hoon . . 15 Perczel Andras . . 16 Tompa Peter . . stop_ _Journal_abbreviation Cell _Journal_volume . _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first . _Page_last . _Year . _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name ERD14 _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label ERD14 $ERD14 stop_ _System_molecular_weight 20786.3 _System_physical_state 'intrinsically disordered' _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details 'single polypeptide chain (protein)' save_ ######################## # Monomeric polymers # ######################## save_ERD14 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common ERD14 _Molecular_mass 20786.3 _Mol_thiol_state 'not present' loop_ _Biological_function chaperone 'stress protein' stop_ _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 185 _Mol_residue_sequence ; MAEEIKNVPEQEVPKVATEE SSAEVTDRGLFDFLGKKKDE TKPEETPIASEFEQKVHISE PEPEVKHESLLEKLHRSDSS SSSSSEEEGSDGEKRKKKKE KKKPTTEVEVKEEEKKGFME KLKEKLPGHKKPEDGSAVAA APVVVPPPVEEAHPVEKKGI LEKIKEKLPGYHPKTTVEEE KKDKE ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 1 MET 2 2 ALA 3 3 GLU 4 4 GLU 5 5 ILE 6 6 LYS 7 7 ASN 8 8 VAL 9 9 PRO 10 10 GLU 11 11 GLN 12 12 GLU 13 13 VAL 14 14 PRO 15 15 LYS 16 16 VAL 17 17 ALA 18 18 THR 19 19 GLU 20 20 GLU 21 21 SER 22 22 SER 23 23 ALA 24 24 GLU 25 25 VAL 26 26 THR 27 27 ASP 28 28 ARG 29 29 GLY 30 30 LEU 31 31 PHE 32 32 ASP 33 33 PHE 34 34 LEU 35 35 GLY 36 36 LYS 37 37 LYS 38 38 LYS 39 39 ASP 40 40 GLU 41 41 THR 42 42 LYS 43 43 PRO 44 44 GLU 45 45 GLU 46 46 THR 47 47 PRO 48 48 ILE 49 49 ALA 50 50 SER 51 51 GLU 52 52 PHE 53 53 GLU 54 54 GLN 55 55 LYS 56 56 VAL 57 57 HIS 58 58 ILE 59 59 SER 60 60 GLU 61 61 PRO 62 62 GLU 63 63 PRO 64 64 GLU 65 65 VAL 66 66 LYS 67 67 HIS 68 68 GLU 69 69 SER 70 70 LEU 71 71 LEU 72 72 GLU 73 73 LYS 74 74 LEU 75 75 HIS 76 76 ARG 77 77 SER 78 78 ASP 79 79 SER 80 80 SER 81 81 SER 82 82 SER 83 83 SER 84 84 SER 85 85 SER 86 86 GLU 87 87 GLU 88 88 GLU 89 89 GLY 90 90 SER 91 91 ASP 92 92 GLY 93 93 GLU 94 94 LYS 95 95 ARG 96 96 LYS 97 97 LYS 98 98 LYS 99 99 LYS 100 100 GLU 101 101 LYS 102 102 LYS 103 103 LYS 104 104 PRO 105 105 THR 106 106 THR 107 107 GLU 108 108 VAL 109 109 GLU 110 110 VAL 111 111 LYS 112 112 GLU 113 113 GLU 114 114 GLU 115 115 LYS 116 116 LYS 117 117 GLY 118 118 PHE 119 119 MET 120 120 GLU 121 121 LYS 122 122 LEU 123 123 LYS 124 124 GLU 125 125 LYS 126 126 LEU 127 127 PRO 128 128 GLY 129 129 HIS 130 130 LYS 131 131 LYS 132 132 PRO 133 133 GLU 134 134 ASP 135 135 GLY 136 136 SER 137 137 ALA 138 138 VAL 139 139 ALA 140 140 ALA 141 141 ALA 142 142 PRO 143 143 VAL 144 144 VAL 145 145 VAL 146 146 PRO 147 147 PRO 148 148 PRO 149 149 VAL 150 150 GLU 151 151 GLU 152 152 ALA 153 153 HIS 154 154 PRO 155 155 VAL 156 156 GLU 157 157 LYS 158 158 LYS 159 159 GLY 160 160 ILE 161 161 LEU 162 162 GLU 163 163 LYS 164 164 ILE 165 165 LYS 166 166 GLU 167 167 LYS 168 168 LEU 169 169 PRO 170 170 GLY 171 171 TYR 172 172 HIS 173 173 PRO 174 174 LYS 175 175 THR 176 176 THR 177 177 VAL 178 178 GLU 179 179 GLU 180 180 GLU 181 181 LYS 182 182 LYS 183 183 ASP 184 184 LYS 185 185 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-09-23 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 16876 ERD14 100.00 185 100.00 100.00 8.57e-118 DBJ BAA04569 "ERD14 protein [Arabidopsis thaliana]" 100.00 185 100.00 100.00 8.57e-118 DBJ BAE98985 "hypothetical protein [Arabidopsis thaliana]" 100.00 185 100.00 100.00 8.57e-118 GB AAF17644 "T23E18.12 [Arabidopsis thaliana]" 100.00 185 100.00 100.00 8.57e-118 GB AAG40050 "At1g76180 [Arabidopsis thaliana]" 100.00 185 99.46 99.46 1.95e-116 GB AAG41486 "putative dehydrin protein [Arabidopsis thaliana]" 100.00 185 100.00 100.00 8.57e-118 GB AAK00404 "putative dehydrin protein [Arabidopsis thaliana]" 100.00 185 100.00 100.00 8.57e-118 GB AAK62649 "At1g76180/T23E18_36 [Arabidopsis thaliana]" 100.00 185 100.00 100.00 8.57e-118 REF NP_001185408 "dehydrin ERD14 [Arabidopsis thaliana]" 100.00 185 100.00 100.00 8.57e-118 REF NP_177745 "dehydrin ERD14 [Arabidopsis thaliana]" 100.00 185 100.00 100.00 8.57e-118 SP P42763 "RecName: Full=Dehydrin ERD14" 100.00 185 100.00 100.00 8.57e-118 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Details $ERD14 'thale cress' 3702 Eukaryota Viridiplantae Arabidopsis thaliana 'Dehydrin ERD14 (Early Response to Dehydration 14)' stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $ERD14 'recombinant technology' . Escherichia coli . pET22b 'Cloned in a way to contain no additional amino acids or tags next to the original ERD14 sequence.' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'Using one single sample, pH was successively adjusted to several different pH values using NaOH/HCl.' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $ERD14 1 mM [U-15N] DSS 1 mM 'natural abundance' 'sodium phosphate' 32 mM 'natural abundance' 'potassium phosphate' 22 mM 'natural abundance' 'sodium chloride' 8.6 mM 'natural abundance' 'calcium chloride' 0.1 mM 'natural abundance' 'magnesium sulfate' 1 mM 'natural abundance' carbenicillin 50 mg/L 'natural abundance' glucose.H2O 4 g/L 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CARA _Saveframe_category software _Name CARA _Version 1.8.4.2 loop_ _Vendor _Address _Electronic_address 'Rochus Keller' 'Rochus Keller & DATONAL AG, CH-6343 Rotkreuz' http://www.nmr.ch stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details ; CARA is the application for the analysis of NMR spectra and computer aided resonance assignment developed at and used by the group of Prof. Dr. Kurt Wuthrich, ETH Zurich. ; save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_288K-6.58pH _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.58 . pH pressure 1 . atm temperature 288 . K stop_ save_ save_282.5K-6.58pH _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.58 . pH pressure 1 . atm temperature 282.5 . K stop_ save_ save_277K-6.58pH _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.58 . pH pressure 1 . atm temperature 277 . K stop_ save_ save_277K-6.94pH _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.94 . pH pressure 1 . atm temperature 277 . K stop_ save_ save_277K-7.29pH _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.29 . pH pressure 1 . atm temperature 277 . K stop_ save_ save_277K-7.42pH _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.42 . pH pressure 1 . atm temperature 277 . K stop_ save_ save_277K-7.57pH _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.57 . pH pressure 1 . atm temperature 277 . K stop_ save_ save_277K-7.70pH _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 7.70 . pH pressure 1 . atm temperature 277 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_Assignment_288K_6.58pH _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CARA stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $288K-6.58pH _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name ERD14 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 4 4 GLU H H 8.696 0.01 1 2 4 4 GLU N N 123.591 0.03 1 3 5 5 ILE H H 8.455 0.01 1 4 5 5 ILE N N 124.176 0.03 1 5 6 6 LYS H H 8.545 0.01 1 6 6 6 LYS N N 126.686 0.03 1 7 7 7 ASN H H 8.568 0.01 1 8 7 7 ASN N N 120.921 0.03 1 9 10 10 GLU H H 8.621 0.01 1 10 10 10 GLU N N 121.362 0.03 1 11 11 11 GLN H H 8.447 0.01 1 12 11 11 GLN N N 121.164 0.03 1 13 12 12 GLU H H 8.511 0.01 1 14 12 12 GLU N N 123.174 0.03 1 15 13 13 VAL H H 8.348 0.01 1 16 13 13 VAL N N 124.153 0.03 1 17 15 15 LYS H H 8.518 0.01 1 18 15 15 LYS N N 122.819 0.03 1 19 17 17 ALA H H 8.568 0.01 1 20 17 17 ALA N N 128.866 0.03 1 21 18 18 THR H H 8.239 0.01 1 22 18 18 THR N N 114.249 0.03 1 23 19 19 GLU H H 8.563 0.01 1 24 19 19 GLU N N 123.389 0.03 1 25 20 20 GLU H H 8.563 0.01 1 26 20 20 GLU N N 122.576 0.03 1 27 21 21 SER H H 8.483 0.01 1 28 21 21 SER N N 117.401 0.03 1 29 22 22 SER H H 8.462 0.01 1 30 22 22 SER N N 118.379 0.03 1 31 23 23 ALA H H 8.365 0.01 1 32 23 23 ALA N N 126.174 0.03 1 33 24 24 GLU H H 8.366 0.01 1 34 24 24 GLU N N 120.224 0.03 1 35 25 25 VAL H H 8.284 0.01 1 36 25 25 VAL N N 121.908 0.03 1 37 26 26 THR H H 8.302 0.01 1 38 26 26 THR N N 118.143 0.03 1 39 27 27 ASP H H 8.394 0.01 1 40 27 27 ASP N N 123.052 0.03 1 41 28 28 ARG H H 8.416 0.01 1 42 28 28 ARG N N 122.271 0.03 1 43 29 29 GLY H H 8.539 0.01 1 44 29 29 GLY N N 109.251 0.03 1 45 30 30 LEU H H 7.985 0.01 1 46 30 30 LEU N N 121.161 0.03 1 47 31 31 PHE H H 8.161 0.01 1 48 31 31 PHE N N 119.434 0.03 1 49 32 32 ASP H H 8.203 0.01 1 50 32 32 ASP N N 121.561 0.03 1 51 33 33 PHE H H 8.12 0.01 1 52 33 33 PHE N N 120.32 0.03 1 53 35 35 GLY H H 8.056 0.01 1 54 35 35 GLY N N 108.947 0.03 1 55 36 36 LYS H H 8.086 0.01 1 56 36 36 LYS N N 121.074 0.03 1 57 37 37 LYS H H 8.502 0.01 1 58 37 37 LYS N N 123.452 0.03 1 59 38 38 LYS H H 8.479 0.01 1 60 38 38 LYS N N 123.639 0.03 1 61 39 39 ASP H H 8.469 0.01 1 62 39 39 ASP N N 122.103 0.03 1 63 41 41 THR H H 8.375 0.01 1 64 41 41 THR N N 116.092 0.03 1 65 44 44 GLU H H 8.685 0.01 1 66 44 44 GLU N N 121.292 0.03 1 67 45 45 GLU H H 8.478 0.01 1 68 45 45 GLU N N 122.555 0.03 1 69 46 46 THR H H 8.385 0.01 1 70 46 46 THR N N 119.281 0.03 1 71 48 48 ILE H H 8.368 0.01 1 72 48 48 ILE N N 122.145 0.03 1 73 49 49 ALA H H 8.493 0.01 1 74 49 49 ALA N N 129.042 0.03 1 75 50 50 SER H H 8.402 0.01 1 76 50 50 SER N N 115.9 0.03 1 77 51 51 GLU H H 8.58 0.01 1 78 51 51 GLU N N 122.893 0.03 1 79 52 52 PHE H H 8.169 0.01 1 80 52 52 PHE N N 120.061 0.03 1 81 53 53 GLU H H 8.176 0.01 1 82 53 53 GLU N N 122.248 0.03 1 83 54 54 GLN H H 8.404 0.01 1 84 54 54 GLN N N 121.937 0.03 1 85 56 56 VAL H H 8.174 0.01 1 86 56 56 VAL N N 121.578 0.03 1 87 57 57 HIS H H 8.643 0.01 1 88 57 57 HIS N N 124.077 0.03 1 89 58 58 ILE H H 8.327 0.01 1 90 58 58 ILE N N 124.502 0.03 1 91 59 59 SER H H 8.495 0.01 1 92 59 59 SER N N 120.86 0.03 1 93 60 60 GLU H H 8.489 0.01 1 94 60 60 GLU N N 124.598 0.03 1 95 62 62 GLU H H 8.591 0.01 1 96 62 62 GLU N N 122.945 0.03 1 97 64 64 GLU H H 8.564 0.01 1 98 64 64 GLU N N 121.845 0.03 1 99 66 66 LYS H H 8.477 0.01 1 100 66 66 LYS N N 125.982 0.03 1 101 67 67 HIS H H 8.635 0.01 1 102 67 67 HIS N N 121.679 0.03 1 103 68 68 GLU H H 8.669 0.01 1 104 68 68 GLU N N 123.055 0.03 1 105 69 69 SER H H 8.627 0.01 1 106 69 69 SER N N 118.04 0.03 1 107 70 70 LEU H H 8.45 0.01 1 108 70 70 LEU N N 124.355 0.03 1 109 71 71 LEU H H 8.108 0.01 1 110 71 71 LEU N N 121.135 0.03 1 111 72 72 GLU H H 8.146 0.01 1 112 72 72 GLU N N 120.886 0.03 1 113 73 73 LYS H H 8.2 0.01 1 114 73 73 LYS N N 121.599 0.03 1 115 74 74 LEU H H 8.13 0.01 1 116 74 74 LEU N N 121.905 0.03 1 117 75 75 HIS H H 8.363 0.01 1 118 75 75 HIS N N 119.383 0.03 1 119 76 76 ARG H H 8.375 0.01 1 120 76 76 ARG N N 122.775 0.03 1 121 77 77 SER H H 8.545 0.01 1 122 77 77 SER N N 117.318 0.03 1 123 78 78 ASP H H 8.454 0.01 1 124 78 78 ASP N N 122.59 0.03 1 125 79 79 SER H H 8.378 0.01 1 126 79 79 SER N N 116.442 0.03 1 127 80 80 SER H H 8.482 0.01 1 128 80 80 SER N N 118.195 0.03 1 129 81 81 SER H H 8.403 0.01 1 130 81 81 SER N N 117.845 0.03 1 131 82 82 SER H H 8.44 0.01 1 132 82 82 SER N N 118.055 0.03 1 133 83 83 SER H H 8.466 0.01 1 134 83 83 SER N N 118.155 0.03 1 135 84 84 SER H H 8.462 0.01 1 136 84 84 SER N N 118.14 0.03 1 137 85 85 SER H H 8.462 0.01 1 138 85 85 SER N N 118.159 0.03 1 139 86 86 GLU H H 8.491 0.01 1 140 86 86 GLU N N 122.679 0.03 1 141 87 87 GLU H H 8.412 0.01 1 142 87 87 GLU N N 121.455 0.03 1 143 89 89 GLY H H 8.548 0.01 1 144 89 89 GLY N N 110.238 0.03 1 145 90 90 SER H H 8.391 0.01 1 146 90 90 SER N N 116.012 0.03 1 147 91 91 ASP H H 8.614 0.01 1 148 91 91 ASP N N 122.129 0.03 1 149 92 92 GLY H H 8.423 0.01 1 150 92 92 GLY N N 108.996 0.03 1 151 94 94 LYS H H 8.335 0.01 1 152 94 94 LYS N N 121.723 0.03 1 153 96 96 LYS H H 8.257 0.01 1 154 96 96 LYS N N 122.355 0.03 1 155 100 100 GLU H H 8.483 0.01 1 156 100 100 GLU N N 123.254 0.03 1 157 102 102 LYS H H 8.472 0.01 1 158 102 102 LYS N N 123.933 0.03 1 159 103 103 LYS H H 8.534 0.01 1 160 103 103 LYS N N 125.34 0.03 1 161 105 105 THR H H 8.46 0.01 1 162 105 105 THR N N 115.237 0.03 1 163 106 106 THR H H 8.186 0.01 1 164 106 106 THR N N 116.03 0.03 1 165 108 108 VAL H H 8.219 0.01 1 166 108 108 VAL N N 121.395 0.03 1 167 109 109 GLU H H 8.473 0.01 1 168 109 109 GLU N N 125.384 0.03 1 169 110 110 VAL H H 8.387 0.01 1 170 110 110 VAL N N 123.683 0.03 1 171 111 111 LYS H H 8.527 0.01 1 172 111 111 LYS N N 126.075 0.03 1 173 112 112 GLU H H 8.583 0.01 1 174 112 112 GLU N N 123.187 0.03 1 175 113 113 GLU H H 8.669 0.01 1 176 113 113 GLU N N 121.602 0.03 1 177 114 114 GLU H H 8.385 0.01 1 178 114 114 GLU N N 121.994 0.03 1 179 115 115 LYS H H 8.285 0.01 1 180 115 115 LYS N N 122.511 0.03 1 181 116 116 LYS H H 8.347 0.01 1 182 116 116 LYS N N 122.338 0.03 1 183 117 117 GLY H H 8.492 0.01 1 184 117 117 GLY N N 109.796 0.03 1 185 118 118 PHE H H 8.128 0.01 1 186 118 118 PHE N N 120.595 0.03 1 187 119 119 MET H H 8.286 0.01 1 188 119 119 MET N N 121.471 0.03 1 189 120 120 GLU H H 8.299 0.01 1 190 120 120 GLU N N 122 0.03 1 191 121 121 LYS H H 8.252 0.01 1 192 121 121 LYS N N 121.96 0.03 1 193 122 122 LEU H H 8.139 0.01 1 194 122 122 LEU N N 122.774 0.03 1 195 123 123 LYS H H 8.204 0.01 1 196 123 123 LYS N N 121.748 0.03 1 197 124 124 GLU H H 8.244 0.01 1 198 124 124 GLU N N 121.574 0.03 1 199 125 125 LYS H H 8.315 0.01 1 200 125 125 LYS N N 122.506 0.03 1 201 126 126 LEU H H 8.338 0.01 1 202 126 126 LEU N N 125.081 0.03 1 203 128 128 GLY H H 8.554 0.01 1 204 128 128 GLY N N 109.304 0.03 1 205 129 129 HIS H H 8.266 0.01 1 206 129 129 HIS N N 119.038 0.03 1 207 130 130 LYS H H 8.411 0.01 1 208 130 130 LYS N N 123.519 0.03 1 209 131 131 LYS H H 8.59 0.01 1 210 131 131 LYS N N 125.298 0.03 1 211 133 133 GLU H H 8.775 0.01 1 212 133 133 GLU N N 121.218 0.03 1 213 134 134 ASP H H 8.366 0.01 1 214 134 134 ASP N N 121.715 0.03 1 215 135 135 GLY H H 8.49 0.01 1 216 135 135 GLY N N 110.145 0.03 1 217 136 136 SER H H 8.276 0.01 1 218 136 136 SER N N 116.033 0.03 1 219 137 137 ALA H H 8.354 0.01 1 220 137 137 ALA N N 125.966 0.03 1 221 138 138 VAL H H 8.094 0.01 1 222 138 138 VAL N N 119.709 0.03 1 223 139 139 ALA H H 8.393 0.01 1 224 139 139 ALA N N 128.473 0.03 1 225 140 140 ALA H H 8.303 0.01 1 226 140 140 ALA N N 124.134 0.03 1 227 141 141 ALA H H 8.314 0.01 1 228 141 141 ALA N N 125.371 0.03 1 229 143 143 VAL H H 8.35 0.01 1 230 143 143 VAL N N 121.805 0.03 1 231 144 144 VAL H H 8.399 0.01 1 232 144 144 VAL N N 126.382 0.03 1 233 145 145 VAL H H 8.482 0.01 1 234 145 145 VAL N N 128.182 0.03 1 235 149 149 VAL H H 8.3 0.01 1 236 149 149 VAL N N 120.998 0.03 1 237 150 150 GLU H H 8.563 0.01 1 238 150 150 GLU N N 125.314 0.03 1 239 151 151 GLU H H 8.491 0.01 1 240 151 151 GLU N N 123.3 0.03 1 241 153 153 HIS H H 8.523 0.01 1 242 153 153 HIS N N 118.919 0.03 1 243 155 155 VAL H H 8.451 0.01 1 244 155 155 VAL N N 121.346 0.03 1 245 156 156 GLU H H 8.595 0.01 1 246 156 156 GLU N N 125.554 0.03 1 247 158 158 LYS H H 8.477 0.01 1 248 158 158 LYS N N 123.197 0.03 1 249 159 159 GLY H H 8.569 0.01 1 250 159 159 GLY N N 110.421 0.03 1 251 160 160 ILE H H 8.067 0.01 1 252 160 160 ILE N N 120.345 0.03 1 253 162 162 GLU H H 8.334 0.01 1 254 162 162 GLU N N 122.143 0.03 1 255 163 163 LYS H H 8.28 0.01 1 256 163 163 LYS N N 122.427 0.03 1 257 166 166 GLU H H 8.331 0.01 1 258 166 166 GLU N N 121.792 0.03 1 259 168 168 LEU H H 8.273 0.01 1 260 168 168 LEU N N 125.023 0.03 1 261 170 170 GLY H H 8.586 0.01 1 262 170 170 GLY N N 109.58 0.03 1 263 171 171 TYR H H 7.98 0.01 1 264 171 171 TYR N N 120.809 0.03 1 265 172 172 HIS H H 8.23 0.01 1 266 172 172 HIS N N 124.547 0.03 1 267 174 174 LYS H H 8.612 0.01 1 268 174 174 LYS N N 122.145 0.03 1 269 175 175 THR H H 8.341 0.01 1 270 175 175 THR N N 116.036 0.03 1 271 176 176 THR H H 8.304 0.01 1 272 176 176 THR N N 117.123 0.03 1 273 178 178 GLU H H 8.535 0.01 1 274 178 178 GLU N N 124.809 0.03 1 275 179 179 GLU H H 8.462 0.01 1 276 179 179 GLU N N 122.467 0.03 1 277 180 180 GLU H H 8.485 0.01 1 278 180 180 GLU N N 122.542 0.03 1 279 183 183 ASP H H 8.439 0.01 1 280 183 183 ASP N N 121.525 0.03 1 281 184 184 LYS H H 8.24 0.01 1 282 184 184 LYS N N 121.185 0.03 1 283 185 185 GLU H H 8.1 0.01 1 284 185 185 GLU N N 127.456 0.03 1 stop_ save_ save_Assignment_282.5K_6.58pH _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CARA stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $282.5K-6.58pH _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name ERD14 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 4 4 GLU H H 8.742 0.01 1 2 4 4 GLU N N 123.738 0.03 1 3 5 5 ILE H H 8.509 0.01 1 4 5 5 ILE N N 124.417 0.03 1 5 6 6 LYS H H 8.595 0.01 1 6 6 6 LYS N N 126.884 0.03 1 7 7 7 ASN H H 8.615 0.01 1 8 7 7 ASN N N 121.036 0.03 1 9 10 10 GLU H H 8.663 0.01 1 10 10 10 GLU N N 121.49 0.03 1 11 11 11 GLN H H 8.497 0.01 1 12 11 11 GLN N N 121.335 0.03 1 13 12 12 GLU H H 8.561 0.01 1 14 12 12 GLU N N 123.338 0.03 1 15 13 13 VAL H H 8.415 0.01 1 16 13 13 VAL N N 124.394 0.03 1 17 15 15 LYS H H 8.568 0.01 1 18 15 15 LYS N N 122.948 0.03 1 19 17 17 ALA H H 8.617 0.01 1 20 17 17 ALA N N 129.054 0.03 1 21 18 18 THR H H 8.294 0.01 1 22 18 18 THR N N 114.436 0.03 1 23 19 19 GLU H H 8.602 0.01 1 24 19 19 GLU N N 123.507 0.03 1 25 20 20 GLU H H 8.607 0.01 1 26 20 20 GLU N N 122.699 0.03 1 27 21 21 SER H H 8.534 0.01 1 28 21 21 SER N N 117.559 0.03 1 29 22 22 SER H H 8.507 0.01 1 30 22 22 SER N N 118.484 0.03 1 31 23 23 ALA H H 8.403 0.01 1 32 23 23 ALA N N 126.238 0.03 1 33 24 24 GLU H H 8.4 0.01 1 34 24 24 GLU N N 120.333 0.03 1 35 26 26 THR H H 8.347 0.01 1 36 26 26 THR N N 118.355 0.03 1 37 27 27 ASP H H 8.435 0.01 1 38 27 27 ASP N N 123.149 0.03 1 39 29 29 GLY H H 8.585 0.01 1 40 29 29 GLY N N 109.376 0.03 1 41 30 30 LEU H H 8.019 0.01 1 42 30 30 LEU N N 121.186 0.03 1 43 31 31 PHE H H 8.201 0.01 1 44 31 31 PHE N N 119.532 0.03 1 45 33 33 PHE H H 8.16 0.01 1 46 33 33 PHE N N 120.436 0.03 1 47 35 35 GLY H H 8.076 0.01 1 48 35 35 GLY N N 109.029 0.03 1 49 36 36 LYS H H 8.126 0.01 1 50 36 36 LYS N N 121.18 0.03 1 51 37 37 LYS H H 8.555 0.01 1 52 37 37 LYS N N 123.648 0.03 1 53 38 38 LYS H H 8.529 0.01 1 54 38 38 LYS N N 123.818 0.03 1 55 39 39 ASP H H 8.514 0.01 1 56 39 39 ASP N N 122.213 0.03 1 57 41 41 THR H H 8.413 0.01 1 58 41 41 THR N N 116.243 0.03 1 59 44 44 GLU H H 8.724 0.01 1 60 44 44 GLU N N 121.428 0.03 1 61 46 46 THR H H 8.444 0.01 1 62 46 46 THR N N 119.517 0.03 1 63 49 49 ALA H H 8.548 0.01 1 64 49 49 ALA N N 129.245 0.03 1 65 50 50 SER H H 8.456 0.01 1 66 50 50 SER N N 116.038 0.03 1 67 52 52 PHE H H 8.209 0.01 1 68 52 52 PHE N N 120.152 0.03 1 69 53 53 GLU H H 8.206 0.01 1 70 53 53 GLU N N 122.36 0.03 1 71 54 54 GLN H H 8.448 0.01 1 72 54 54 GLN N N 122.088 0.03 1 73 57 57 HIS H H 8.707 0.01 1 74 57 57 HIS N N 124.244 0.03 1 75 58 58 ILE H H 8.391 0.01 1 76 58 58 ILE N N 124.781 0.03 1 77 59 59 SER H H 8.545 0.01 1 78 59 59 SER N N 121.067 0.03 1 79 60 60 GLU H H 8.545 0.01 1 80 60 60 GLU N N 124.705 0.03 1 81 64 64 GLU H H 8.608 0.01 1 82 64 64 GLU N N 122.028 0.03 1 83 66 66 LYS H H 8.528 0.01 1 84 66 66 LYS N N 126.155 0.03 1 85 67 67 HIS H H 8.697 0.01 1 86 67 67 HIS N N 121.754 0.03 1 87 68 68 GLU H H 8.71 0.01 1 88 68 68 GLU N N 123.231 0.03 1 89 69 69 SER H H 8.688 0.01 1 90 69 69 SER N N 118.308 0.03 1 91 70 70 LEU H H 8.513 0.01 1 92 70 70 LEU N N 124.389 0.03 1 93 71 71 LEU H H 8.143 0.01 1 94 71 71 LEU N N 121.18 0.03 1 95 72 72 GLU H H 8.172 0.01 1 96 72 72 GLU N N 121.046 0.03 1 97 74 74 LEU H H 8.174 0.01 1 98 74 74 LEU N N 122.021 0.03 1 99 75 75 HIS H H 8.404 0.01 1 100 75 75 HIS N N 119.377 0.03 1 101 76 76 ARG H H 8.422 0.01 1 102 76 76 ARG N N 122.88 0.03 1 103 77 77 SER H H 8.583 0.01 1 104 77 77 SER N N 117.352 0.03 1 105 79 79 SER H H 8.41 0.01 1 106 79 79 SER N N 116.515 0.03 1 107 80 80 SER H H 8.512 0.01 1 108 80 80 SER N N 118.265 0.03 1 109 81 81 SER H H 8.437 0.01 1 110 81 81 SER N N 117.926 0.03 1 111 82 82 SER H H 8.469 0.01 1 112 82 82 SER N N 118.122 0.03 1 113 83 83 SER H H 8.5 0.01 1 114 83 83 SER N N 118.225 0.03 1 115 84 84 SER H H 8.497 0.01 1 116 84 84 SER N N 118.206 0.03 1 117 85 85 SER H H 8.496 0.01 1 118 85 85 SER N N 118.226 0.03 1 119 87 87 GLU H H 8.448 0.01 1 120 87 87 GLU N N 121.527 0.03 1 121 89 89 GLY H H 8.588 0.01 1 122 89 89 GLY N N 110.337 0.03 1 123 90 90 SER H H 8.426 0.01 1 124 90 90 SER N N 116.101 0.03 1 125 91 91 ASP H H 8.652 0.01 1 126 91 91 ASP N N 122.172 0.03 1 127 92 92 GLY H H 8.453 0.01 1 128 92 92 GLY N N 109.043 0.03 1 129 102 102 LYS H H 8.516 0.01 1 130 102 102 LYS N N 124.095 0.03 1 131 103 103 LYS H H 8.59 0.01 1 132 103 103 LYS N N 125.52 0.03 1 133 105 105 THR H H 8.514 0.01 1 134 105 105 THR N N 115.439 0.03 1 135 106 106 THR H H 8.232 0.01 1 136 106 106 THR N N 116.198 0.03 1 137 109 109 GLU H H 8.512 0.01 1 138 109 109 GLU N N 125.541 0.03 1 139 110 110 VAL H H 8.445 0.01 1 140 110 110 VAL N N 123.92 0.03 1 141 111 111 LYS H H 8.563 0.01 1 142 111 111 LYS N N 126.192 0.03 1 143 112 112 GLU H H 8.621 0.01 1 144 112 112 GLU N N 123.298 0.03 1 145 113 113 GLU H H 8.701 0.01 1 146 113 113 GLU N N 121.717 0.03 1 147 114 114 GLU H H 8.415 0.01 1 148 114 114 GLU N N 122.115 0.03 1 149 117 117 GLY H H 8.532 0.01 1 150 117 117 GLY N N 109.911 0.03 1 151 118 118 PHE H H 8.159 0.01 1 152 118 118 PHE N N 120.674 0.03 1 153 122 122 LEU H H 8.183 0.01 1 154 122 122 LEU N N 122.953 0.03 1 155 126 126 LEU H H 8.395 0.01 1 156 126 126 LEU N N 125.246 0.03 1 157 128 128 GLY H H 8.576 0.01 1 158 128 128 GLY N N 109.328 0.03 1 159 129 129 HIS H H 8.323 0.01 1 160 129 129 HIS N N 119.026 0.03 1 161 130 130 LYS H H 8.467 0.01 1 162 130 130 LYS N N 123.677 0.03 1 163 131 131 LYS H H 8.645 0.01 1 164 131 131 LYS N N 125.463 0.03 1 165 133 133 GLU H H 8.815 0.01 1 166 133 133 GLU N N 121.341 0.03 1 167 134 134 ASP H H 8.408 0.01 1 168 134 134 ASP N N 121.823 0.03 1 169 135 135 GLY H H 8.53 0.01 1 170 135 135 GLY N N 110.274 0.03 1 171 136 136 SER H H 8.303 0.01 1 172 136 136 SER N N 116.101 0.03 1 173 137 137 ALA H H 8.39 0.01 1 174 137 137 ALA N N 126.026 0.03 1 175 138 138 VAL H H 8.149 0.01 1 176 138 138 VAL N N 119.956 0.03 1 177 139 139 ALA H H 8.449 0.01 1 178 139 139 ALA N N 128.682 0.03 1 179 140 140 ALA H H 8.361 0.01 1 180 140 140 ALA N N 124.253 0.03 1 181 141 141 ALA H H 8.369 0.01 1 182 141 141 ALA N N 125.498 0.03 1 183 144 144 VAL H H 8.462 0.01 1 184 144 144 VAL N N 126.693 0.03 1 185 145 145 VAL H H 8.556 0.01 1 186 145 145 VAL N N 128.558 0.03 1 187 149 149 VAL H H 8.359 0.01 1 188 149 149 VAL N N 121.213 0.03 1 189 150 150 GLU H H 8.612 0.01 1 190 150 150 GLU N N 125.53 0.03 1 191 153 153 HIS H H 8.585 0.01 1 192 153 153 HIS N N 118.912 0.03 1 193 155 155 VAL H H 8.498 0.01 1 194 155 155 VAL N N 121.547 0.03 1 195 156 156 GLU H H 8.64 0.01 1 196 156 156 GLU N N 125.789 0.03 1 197 159 159 GLY H H 8.608 0.01 1 198 159 159 GLY N N 110.543 0.03 1 199 160 160 ILE H H 8.115 0.01 1 200 160 160 ILE N N 120.462 0.03 1 201 168 168 LEU H H 8.326 0.01 1 202 168 168 LEU N N 125.158 0.03 1 203 170 170 GLY H H 8.637 0.01 1 204 170 170 GLY N N 109.696 0.03 1 205 171 171 TYR H H 8.017 0.01 1 206 171 171 TYR N N 120.891 0.03 1 207 172 172 HIS H H 8.276 0.01 1 208 172 172 HIS N N 124.668 0.03 1 209 174 174 LYS H H 8.654 0.01 1 210 174 174 LYS N N 122.241 0.03 1 211 175 175 THR H H 8.39 0.01 1 212 175 175 THR N N 116.246 0.03 1 213 176 176 THR H H 8.348 0.01 1 214 176 176 THR N N 117.264 0.03 1 215 178 178 GLU H H 8.573 0.01 1 216 178 178 GLU N N 124.926 0.03 1 217 183 183 ASP H H 8.48 0.01 1 218 183 183 ASP N N 121.614 0.03 1 219 184 184 LYS H H 8.28 0.01 1 220 184 184 LYS N N 121.274 0.03 1 221 185 185 GLU H H 8.144 0.01 1 222 185 185 GLU N N 127.545 0.03 1 stop_ save_ save_Assignment_277K_6.58pH _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CARA stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $277K-6.58pH _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name ERD14 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 4 4 GLU H H 8.787 0.01 1 2 4 4 GLU N N 123.889 0.03 1 3 5 5 ILE H H 8.569 0.01 1 4 5 5 ILE N N 124.643 0.03 1 5 6 6 LYS H H 8.647 0.01 1 6 6 6 LYS N N 127.097 0.03 1 7 7 7 ASN H H 8.663 0.01 1 8 7 7 ASN N N 121.146 0.03 1 9 10 10 GLU H H 8.704 0.01 1 10 10 10 GLU N N 121.613 0.03 1 11 11 11 GLN H H 8.549 0.01 1 12 11 11 GLN N N 121.547 0.03 1 13 12 12 GLU H H 8.613 0.01 1 14 12 12 GLU N N 123.499 0.03 1 15 13 13 VAL H H 8.483 0.01 1 16 13 13 VAL N N 124.651 0.03 1 17 15 15 LYS H H 8.618 0.01 1 18 15 15 LYS N N 123.07 0.03 1 19 17 17 ALA H H 8.668 0.01 1 20 17 17 ALA N N 129.236 0.03 1 21 18 18 THR H H 8.349 0.01 1 22 18 18 THR N N 114.632 0.03 1 23 19 19 GLU H H 8.645 0.01 1 24 19 19 GLU N N 123.634 0.03 1 25 20 20 GLU H H 8.652 0.01 1 26 20 20 GLU N N 122.822 0.03 1 27 21 21 SER H H 8.586 0.01 1 28 21 21 SER N N 117.715 0.03 1 29 22 22 SER H H 8.555 0.01 1 30 22 22 SER N N 118.601 0.03 1 31 23 23 ALA H H 8.44 0.01 1 32 23 23 ALA N N 126.298 0.03 1 33 24 24 GLU H H 8.433 0.01 1 34 24 24 GLU N N 120.451 0.03 1 35 26 26 THR H H 8.394 0.01 1 36 26 26 THR N N 118.584 0.03 1 37 27 27 ASP H H 8.479 0.01 1 38 27 27 ASP N N 123.237 0.03 1 39 29 29 GLY H H 8.633 0.01 1 40 29 29 GLY N N 109.504 0.03 1 41 30 30 LEU H H 8.054 0.01 1 42 30 30 LEU N N 121.191 0.03 1 43 31 31 PHE H H 8.241 0.01 1 44 31 31 PHE N N 119.644 0.03 1 45 33 33 PHE H H 8.201 0.01 1 46 33 33 PHE N N 120.542 0.03 1 47 35 35 GLY H H 8.096 0.01 1 48 35 35 GLY N N 109.109 0.03 1 49 36 36 LYS H H 8.163 0.01 1 50 36 36 LYS N N 121.278 0.03 1 51 37 37 LYS H H 8.608 0.01 1 52 37 37 LYS N N 123.831 0.03 1 53 38 38 LYS H H 8.583 0.01 1 54 38 38 LYS N N 124.001 0.03 1 55 39 39 ASP H H 8.558 0.01 1 56 39 39 ASP N N 122.329 0.03 1 57 41 41 THR H H 8.454 0.01 1 58 41 41 THR N N 116.42 0.03 1 59 44 44 GLU H H 8.76 0.01 1 60 44 44 GLU N N 121.572 0.03 1 61 46 46 THR H H 8.505 0.01 1 62 46 46 THR N N 119.759 0.03 1 63 49 49 ALA H H 8.602 0.01 1 64 49 49 ALA N N 129.447 0.03 1 65 50 50 SER H H 8.51 0.01 1 66 50 50 SER N N 116.172 0.03 1 67 52 52 PHE H H 8.249 0.01 1 68 52 52 PHE N N 120.248 0.03 1 69 53 53 GLU H H 8.232 0.01 1 70 53 53 GLU N N 122.499 0.03 1 71 54 54 GLN H H 8.496 0.01 1 72 54 54 GLN N N 122.247 0.03 1 73 57 57 HIS H H 8.768 0.01 1 74 57 57 HIS N N 124.415 0.03 1 75 58 58 ILE H H 8.456 0.01 1 76 58 58 ILE N N 125.061 0.03 1 77 59 59 SER H H 8.599 0.01 1 78 59 59 SER N N 121.274 0.03 1 79 60 60 GLU H H 8.6 0.01 1 80 60 60 GLU N N 124.808 0.03 1 81 64 64 GLU H H 8.651 0.01 1 82 64 64 GLU N N 122.197 0.03 1 83 66 66 LYS H H 8.578 0.01 1 84 66 66 LYS N N 126.331 0.03 1 85 67 67 HIS H H 8.76 0.01 1 86 67 67 HIS N N 121.841 0.03 1 87 68 68 GLU H H 8.751 0.01 1 88 68 68 GLU N N 123.418 0.03 1 89 69 69 SER H H 8.753 0.01 1 90 69 69 SER N N 118.597 0.03 1 91 70 70 LEU H H 8.57 0.01 1 92 70 70 LEU N N 124.415 0.03 1 93 71 71 LEU H H 8.186 0.01 1 94 71 71 LEU N N 121.241 0.03 1 95 72 72 GLU H H 8.199 0.01 1 96 72 72 GLU N N 121.2 0.03 1 97 74 74 LEU H H 8.219 0.01 1 98 74 74 LEU N N 122.127 0.03 1 99 75 75 HIS H H 8.442 0.01 1 100 75 75 HIS N N 119.354 0.03 1 101 76 76 ARG H H 8.472 0.01 1 102 76 76 ARG N N 122.981 0.03 1 103 77 77 SER H H 8.617 0.01 1 104 77 77 SER N N 117.38 0.03 1 105 79 79 SER H H 8.443 0.01 1 106 79 79 SER N N 116.59 0.03 1 107 80 80 SER H H 8.544 0.01 1 108 80 80 SER N N 118.335 0.03 1 109 81 81 SER H H 8.471 0.01 1 110 81 81 SER N N 118.009 0.03 1 111 82 82 SER H H 8.504 0.01 1 112 82 82 SER N N 118.196 0.03 1 113 83 83 SER H H 8.533 0.01 1 114 83 83 SER N N 118.297 0.03 1 115 84 84 SER H H 8.53 0.01 1 116 84 84 SER N N 118.285 0.03 1 117 85 85 SER H H 8.529 0.01 1 118 85 85 SER N N 118.297 0.03 1 119 87 87 GLU H H 8.479 0.01 1 120 87 87 GLU N N 121.589 0.03 1 121 89 89 GLY H H 8.63 0.01 1 122 89 89 GLY N N 110.447 0.03 1 123 90 90 SER H H 8.467 0.01 1 124 90 90 SER N N 116.18 0.03 1 125 91 91 ASP H H 8.689 0.01 1 126 91 91 ASP N N 122.205 0.03 1 127 92 92 GLY H H 8.485 0.01 1 128 92 92 GLY N N 109.079 0.03 1 129 102 102 LYS H H 8.559 0.01 1 130 102 102 LYS N N 124.266 0.03 1 131 103 103 LYS H H 8.644 0.01 1 132 103 103 LYS N N 125.694 0.03 1 133 105 105 THR H H 8.569 0.01 1 134 105 105 THR N N 115.646 0.03 1 135 106 106 THR H H 8.279 0.01 1 136 106 106 THR N N 116.379 0.03 1 137 109 109 GLU H H 8.551 0.01 1 138 109 109 GLU N N 125.702 0.03 1 139 110 110 VAL H H 8.501 0.01 1 140 110 110 VAL N N 124.152 0.03 1 141 111 111 LYS H H 8.596 0.01 1 142 111 111 LYS N N 126.327 0.03 1 143 112 112 GLU H H 8.661 0.01 1 144 112 112 GLU N N 123.405 0.03 1 145 113 113 GLU H H 8.733 0.01 1 146 113 113 GLU N N 121.8 0.03 1 147 114 114 GLU H H 8.45 0.01 1 148 114 114 GLU N N 122.23 0.03 1 149 117 117 GLY H H 8.575 0.01 1 150 117 117 GLY N N 110.036 0.03 1 151 118 118 PHE H H 8.188 0.01 1 152 118 118 PHE N N 120.777 0.03 1 153 122 122 LEU H H 8.23 0.01 1 154 122 122 LEU N N 123.145 0.03 1 155 126 126 LEU H H 8.454 0.01 1 156 126 126 LEU N N 125.412 0.03 1 157 128 128 GLY H H 8.598 0.01 1 158 128 128 GLY N N 109.346 0.03 1 159 129 129 HIS H H 8.382 0.01 1 160 129 129 HIS N N 119.01 0.03 1 161 130 130 LYS H H 8.536 0.01 1 162 130 130 LYS N N 123.857 0.03 1 163 131 131 LYS H H 8.697 0.01 1 164 131 131 LYS N N 125.615 0.03 1 165 133 133 GLU H H 8.855 0.01 1 166 133 133 GLU N N 121.456 0.03 1 167 134 134 ASP H H 8.455 0.01 1 168 134 134 ASP N N 121.94 0.03 1 169 135 135 GLY H H 8.573 0.01 1 170 135 135 GLY N N 110.402 0.03 1 171 136 136 SER H H 8.331 0.01 1 172 136 136 SER N N 116.155 0.03 1 173 137 137 ALA H H 8.428 0.01 1 174 137 137 ALA N N 126.087 0.03 1 175 138 138 VAL H H 8.205 0.01 1 176 138 138 VAL N N 120.223 0.03 1 177 139 139 ALA H H 8.507 0.01 1 178 139 139 ALA N N 128.888 0.03 1 179 140 140 ALA H H 8.419 0.01 1 180 140 140 ALA N N 124.374 0.03 1 181 141 141 ALA H H 8.427 0.01 1 182 141 141 ALA N N 125.611 0.03 1 183 144 144 VAL H H 8.527 0.01 1 184 144 144 VAL N N 127.014 0.03 1 185 145 145 VAL H H 8.629 0.01 1 186 145 145 VAL N N 128.938 0.03 1 187 149 149 VAL H H 8.417 0.01 1 188 149 149 VAL N N 121.427 0.03 1 189 150 150 GLU H H 8.658 0.01 1 190 150 150 GLU N N 125.735 0.03 1 191 153 153 HIS H H 8.647 0.01 1 192 153 153 HIS N N 118.919 0.03 1 193 155 155 VAL H H 8.549 0.01 1 194 155 155 VAL N N 121.704 0.03 1 195 156 156 GLU H H 8.686 0.01 1 196 156 156 GLU N N 126.016 0.03 1 197 159 159 GLY H H 8.65 0.01 1 198 159 159 GLY N N 110.668 0.03 1 199 160 160 ILE H H 8.166 0.01 1 200 160 160 ILE N N 120.575 0.03 1 201 168 168 LEU H H 8.378 0.01 1 202 168 168 LEU N N 125.296 0.03 1 203 170 170 GLY H H 8.686 0.01 1 204 170 170 GLY N N 109.81 0.03 1 205 171 171 TYR H H 8.056 0.01 1 206 171 171 TYR N N 120.976 0.03 1 207 172 172 HIS H H 8.32 0.01 1 208 172 172 HIS N N 124.804 0.03 1 209 174 174 LYS H H 8.698 0.01 1 210 174 174 LYS N N 122.346 0.03 1 211 175 175 THR H H 8.435 0.01 1 212 175 175 THR N N 116.42 0.03 1 213 176 176 THR H H 8.393 0.01 1 214 176 176 THR N N 117.421 0.03 1 215 178 178 GLU H H 8.612 0.01 1 216 178 178 GLU N N 125.048 0.03 1 217 183 183 ASP H H 8.52 0.01 1 218 183 183 ASP N N 121.717 0.03 1 219 184 184 LYS H H 8.322 0.01 1 220 184 184 LYS N N 121.391 0.03 1 221 185 185 GLU H H 8.188 0.01 1 222 185 185 GLU N N 127.635 0.03 1 stop_ save_ save_Assignment_277K_6.94pH _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CARA stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $277K-6.94pH _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name ERD14 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 4 4 GLU H H 8.789 0.01 1 2 4 4 GLU N N 123.889 0.03 1 3 5 5 ILE H H 8.571 0.01 1 4 5 5 ILE N N 124.646 0.03 1 5 6 6 LYS H H 8.649 0.01 1 6 6 6 LYS N N 127.094 0.03 1 7 7 7 ASN H H 8.667 0.01 1 8 7 7 ASN N N 121.146 0.03 1 9 10 10 GLU H H 8.707 0.01 1 10 10 10 GLU N N 121.619 0.03 1 11 11 11 GLN H H 8.552 0.01 1 12 11 11 GLN N N 121.56 0.03 1 13 12 12 GLU H H 8.615 0.01 1 14 12 12 GLU N N 123.499 0.03 1 15 13 13 VAL H H 8.486 0.01 1 16 13 13 VAL N N 124.651 0.03 1 17 15 15 LYS H H 8.621 0.01 1 18 15 15 LYS N N 123.07 0.03 1 19 17 17 ALA H H 8.67 0.01 1 20 17 17 ALA N N 129.236 0.03 1 21 18 18 THR H H 8.352 0.01 1 22 18 18 THR N N 114.637 0.03 1 23 19 19 GLU H H 8.647 0.01 1 24 19 19 GLU N N 123.634 0.03 1 25 20 20 GLU H H 8.655 0.01 1 26 20 20 GLU N N 122.822 0.03 1 27 21 21 SER H H 8.589 0.01 1 28 21 21 SER N N 117.729 0.03 1 29 22 22 SER H H 8.558 0.01 1 30 22 22 SER N N 118.601 0.03 1 31 23 23 ALA H H 8.442 0.01 1 32 23 23 ALA N N 126.298 0.03 1 33 24 24 GLU H H 8.436 0.01 1 34 24 24 GLU N N 120.451 0.03 1 35 26 26 THR H H 8.397 0.01 1 36 26 26 THR N N 118.595 0.03 1 37 27 27 ASP H H 8.482 0.01 1 38 27 27 ASP N N 123.253 0.03 1 39 29 29 GLY H H 8.639 0.01 1 40 29 29 GLY N N 109.508 0.03 1 41 30 30 LEU H H 8.055 0.01 1 42 30 30 LEU N N 121.194 0.03 1 43 31 31 PHE H H 8.242 0.01 1 44 31 31 PHE N N 119.651 0.03 1 45 33 33 PHE H H 8.203 0.01 1 46 33 33 PHE N N 120.539 0.03 1 47 35 35 GLY H H 8.096 0.01 1 48 35 35 GLY N N 109.105 0.03 1 49 36 36 LYS H H 8.163 0.01 1 50 36 36 LYS N N 121.281 0.03 1 51 37 37 LYS H H 8.608 0.01 1 52 37 37 LYS N N 123.787 0.03 1 53 38 38 LYS H H 8.583 0.01 1 54 38 38 LYS N N 124.001 0.03 1 55 39 39 ASP H H 8.561 0.01 1 56 39 39 ASP N N 122.329 0.03 1 57 41 41 THR H H 8.454 0.01 1 58 41 41 THR N N 116.429 0.03 1 59 44 44 GLU H H 8.763 0.01 1 60 44 44 GLU N N 121.575 0.03 1 61 46 46 THR H H 8.508 0.01 1 62 46 46 THR N N 119.777 0.03 1 63 49 49 ALA H H 8.605 0.01 1 64 49 49 ALA N N 129.453 0.03 1 65 50 50 SER H H 8.513 0.01 1 66 50 50 SER N N 116.177 0.03 1 67 52 52 PHE H H 8.251 0.01 1 68 52 52 PHE N N 120.259 0.03 1 69 53 53 GLU H H 8.235 0.01 1 70 53 53 GLU N N 122.527 0.03 1 71 54 54 GLN H H 8.502 0.01 1 72 54 54 GLN N N 122.316 0.03 1 73 57 57 HIS H H 8.74 0.01 1 74 57 57 HIS N N 124.725 0.03 1 75 58 58 ILE H H 8.424 0.01 1 76 58 58 ILE N N 125.097 0.03 1 77 59 59 SER H H 8.591 0.01 1 78 59 59 SER N N 121.302 0.03 1 79 60 60 GLU H H 8.603 0.01 1 80 60 60 GLU N N 124.821 0.03 1 81 64 64 GLU H H 8.656 0.01 1 82 64 64 GLU N N 122.197 0.03 1 83 66 66 LYS H H 8.583 0.01 1 84 66 66 LYS N N 126.426 0.03 1 85 67 67 HIS H H 8.718 0.01 1 86 67 67 HIS N N 122.168 0.03 1 87 68 68 GLU H H 8.731 0.01 1 88 68 68 GLU N N 123.431 0.03 1 89 69 69 SER H H 8.747 0.01 1 90 69 69 SER N N 118.489 0.03 1 91 70 70 LEU H H 8.554 0.01 1 92 70 70 LEU N N 124.396 0.03 1 93 71 71 LEU H H 8.179 0.01 1 94 71 71 LEU N N 121.301 0.03 1 95 72 72 GLU H H 8.21 0.01 1 96 72 72 GLU N N 121.227 0.03 1 97 74 74 LEU H H 8.229 0.01 1 98 74 74 LEU N N 122.327 0.03 1 99 75 75 HIS H H 8.429 0.01 1 100 75 75 HIS N N 119.693 0.03 1 101 76 76 ARG H H 8.462 0.01 1 102 76 76 ARG N N 123.025 0.03 1 103 77 77 SER H H 8.626 0.01 1 104 77 77 SER N N 117.452 0.03 1 105 79 79 SER H H 8.446 0.01 1 106 79 79 SER N N 116.614 0.03 1 107 80 80 SER H H 8.546 0.01 1 108 80 80 SER N N 118.355 0.03 1 109 81 81 SER H H 8.476 0.01 1 110 81 81 SER N N 118.02 0.03 1 111 82 82 SER H H 8.508 0.01 1 112 82 82 SER N N 118.211 0.03 1 113 83 83 SER H H 8.538 0.01 1 114 83 83 SER N N 118.307 0.03 1 115 84 84 SER H H 8.535 0.01 1 116 84 84 SER N N 118.298 0.03 1 117 85 85 SER H H 8.533 0.01 1 118 85 85 SER N N 118.311 0.03 1 119 87 87 GLU H H 8.482 0.01 1 120 87 87 GLU N N 121.596 0.03 1 121 89 89 GLY H H 8.631 0.01 1 122 89 89 GLY N N 110.453 0.03 1 123 90 90 SER H H 8.471 0.01 1 124 90 90 SER N N 116.194 0.03 1 125 91 91 ASP H H 8.693 0.01 1 126 91 91 ASP N N 122.214 0.03 1 127 92 92 GLY H H 8.486 0.01 1 128 92 92 GLY N N 109.089 0.03 1 129 102 102 LYS H H 8.559 0.01 1 130 102 102 LYS N N 124.266 0.03 1 131 103 103 LYS H H 8.645 0.01 1 132 103 103 LYS N N 125.682 0.03 1 133 105 105 THR H H 8.577 0.01 1 134 105 105 THR N N 115.67 0.03 1 135 106 106 THR H H 8.287 0.01 1 136 106 106 THR N N 116.431 0.03 1 137 109 109 GLU H H 8.557 0.01 1 138 109 109 GLU N N 125.724 0.03 1 139 110 110 VAL H H 8.503 0.01 1 140 110 110 VAL N N 124.154 0.03 1 141 111 111 LYS H H 8.606 0.01 1 142 111 111 LYS N N 126.371 0.03 1 143 112 112 GLU H H 8.662 0.01 1 144 112 112 GLU N N 123.413 0.03 1 145 113 113 GLU H H 8.738 0.01 1 146 113 113 GLU N N 121.816 0.03 1 147 114 114 GLU H H 8.455 0.01 1 148 114 114 GLU N N 122.262 0.03 1 149 117 117 GLY H H 8.579 0.01 1 150 117 117 GLY N N 110.05 0.03 1 151 118 118 PHE H H 8.191 0.01 1 152 118 118 PHE N N 120.77 0.03 1 153 122 122 LEU H H 8.238 0.01 1 154 122 122 LEU N N 123.195 0.03 1 155 126 126 LEU H H 8.464 0.01 1 156 126 126 LEU N N 125.459 0.03 1 157 128 128 GLY H H 8.601 0.01 1 158 128 128 GLY N N 109.346 0.03 1 159 129 129 HIS H H 8.329 0.01 1 160 129 129 HIS N N 119.28 0.03 1 161 130 130 LYS H H 8.498 0.01 1 162 130 130 LYS N N 123.857 0.03 1 163 131 131 LYS H H 8.688 0.01 1 164 131 131 LYS N N 125.596 0.03 1 165 133 133 GLU H H 8.855 0.01 1 166 133 133 GLU N N 121.481 0.03 1 167 134 134 ASP H H 8.459 0.01 1 168 134 134 ASP N N 121.941 0.03 1 169 135 135 GLY H H 8.576 0.01 1 170 135 135 GLY N N 110.404 0.03 1 171 136 136 SER H H 8.334 0.01 1 172 136 136 SER N N 116.16 0.03 1 173 137 137 ALA H H 8.429 0.01 1 174 137 137 ALA N N 126.078 0.03 1 175 138 138 VAL H H 8.206 0.01 1 176 138 138 VAL N N 120.216 0.03 1 177 139 139 ALA H H 8.508 0.01 1 178 139 139 ALA N N 128.894 0.03 1 179 140 140 ALA H H 8.421 0.01 1 180 140 140 ALA N N 124.365 0.03 1 181 141 141 ALA H H 8.429 0.01 1 182 141 141 ALA N N 125.611 0.03 1 183 144 144 VAL H H 8.528 0.01 1 184 144 144 VAL N N 127.02 0.03 1 185 145 145 VAL H H 8.631 0.01 1 186 145 145 VAL N N 128.944 0.03 1 187 149 149 VAL H H 8.419 0.01 1 188 149 149 VAL N N 121.43 0.03 1 189 150 150 GLU H H 8.66 0.01 1 190 150 150 GLU N N 125.726 0.03 1 191 153 153 HIS H H 8.616 0.01 1 192 153 153 HIS N N 119.269 0.03 1 193 155 155 VAL H H 8.549 0.01 1 194 155 155 VAL N N 121.676 0.03 1 195 156 156 GLU H H 8.685 0.01 1 196 156 156 GLU N N 125.944 0.03 1 197 159 159 GLY H H 8.651 0.01 1 198 159 159 GLY N N 110.658 0.03 1 199 160 160 ILE H H 8.167 0.01 1 200 160 160 ILE N N 120.568 0.03 1 201 168 168 LEU H H 8.392 0.01 1 202 168 168 LEU N N 125.368 0.03 1 203 170 170 GLY H H 8.693 0.01 1 204 170 170 GLY N N 109.841 0.03 1 205 171 171 TYR H H 8.05 0.01 1 206 171 171 TYR N N 120.912 0.03 1 207 172 172 HIS H H 8.307 0.01 1 208 172 172 HIS N N 124.964 0.03 1 209 174 174 LYS H H 8.713 0.01 1 210 174 174 LYS N N 122.321 0.03 1 211 175 175 THR H H 8.435 0.01 1 212 175 175 THR N N 116.429 0.03 1 213 176 176 THR H H 8.396 0.01 1 214 176 176 THR N N 117.492 0.03 1 215 178 178 GLU H H 8.619 0.01 1 216 178 178 GLU N N 125.108 0.03 1 217 183 183 ASP H H 8.523 0.01 1 218 183 183 ASP N N 121.73 0.03 1 219 184 184 LYS H H 8.327 0.01 1 220 184 184 LYS N N 121.404 0.03 1 221 185 185 GLU H H 8.191 0.01 1 222 185 185 GLU N N 127.648 0.03 1 stop_ save_ save_Assignment_277K_7.29pH _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CARA stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $277K-7.29pH _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name ERD14 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 4 4 GLU H H 8.788 0.01 1 2 4 4 GLU N N 123.886 0.03 1 3 5 5 ILE H H 8.57 0.01 1 4 5 5 ILE N N 124.646 0.03 1 5 6 6 LYS H H 8.648 0.01 1 6 6 6 LYS N N 127.091 0.03 1 7 7 7 ASN H H 8.667 0.01 1 8 7 7 ASN N N 121.14 0.03 1 9 10 10 GLU H H 8.706 0.01 1 10 10 10 GLU N N 121.613 0.03 1 11 11 11 GLN H H 8.55 0.01 1 12 11 11 GLN N N 121.572 0.03 1 13 12 12 GLU H H 8.614 0.01 1 14 12 12 GLU N N 123.505 0.03 1 15 13 13 VAL H H 8.486 0.01 1 16 13 13 VAL N N 124.657 0.03 1 17 15 15 LYS H H 8.62 0.01 1 18 15 15 LYS N N 123.07 0.03 1 19 17 17 ALA H H 8.669 0.01 1 20 17 17 ALA N N 129.236 0.03 1 21 18 18 THR H H 8.352 0.01 1 22 18 18 THR N N 114.646 0.03 1 23 19 19 GLU H H 8.646 0.01 1 24 19 19 GLU N N 123.64 0.03 1 25 20 20 GLU H H 8.654 0.01 1 26 20 20 GLU N N 122.822 0.03 1 27 21 21 SER H H 8.588 0.01 1 28 21 21 SER N N 117.724 0.03 1 29 22 22 SER H H 8.559 0.01 1 30 22 22 SER N N 118.606 0.03 1 31 23 23 ALA H H 8.443 0.01 1 32 23 23 ALA N N 126.298 0.03 1 33 24 24 GLU H H 8.437 0.01 1 34 24 24 GLU N N 120.451 0.03 1 35 26 26 THR H H 8.396 0.01 1 36 26 26 THR N N 118.598 0.03 1 37 27 27 ASP H H 8.481 0.01 1 38 27 27 ASP N N 123.249 0.03 1 39 29 29 GLY H H 8.643 0.01 1 40 29 29 GLY N N 109.512 0.03 1 41 30 30 LEU H H 8.054 0.01 1 42 30 30 LEU N N 121.194 0.03 1 43 31 31 PHE H H 8.241 0.01 1 44 31 31 PHE N N 119.644 0.03 1 45 33 33 PHE H H 8.203 0.01 1 46 33 33 PHE N N 120.54 0.03 1 47 35 35 GLY H H 8.094 0.01 1 48 35 35 GLY N N 109.101 0.03 1 49 36 36 LYS H H 8.164 0.01 1 50 36 36 LYS N N 121.278 0.03 1 51 37 37 LYS H H 8.605 0.01 1 52 37 37 LYS N N 123.718 0.03 1 53 38 38 LYS H H 8.583 0.01 1 54 38 38 LYS N N 124.001 0.03 1 55 39 39 ASP H H 8.56 0.01 1 56 39 39 ASP N N 122.335 0.03 1 57 41 41 THR H H 8.455 0.01 1 58 41 41 THR N N 116.425 0.03 1 59 44 44 GLU H H 8.762 0.01 1 60 44 44 GLU N N 121.572 0.03 1 61 46 46 THR H H 8.508 0.01 1 62 46 46 THR N N 119.787 0.03 1 63 49 49 ALA H H 8.604 0.01 1 64 49 49 ALA N N 129.459 0.03 1 65 50 50 SER H H 8.513 0.01 1 66 50 50 SER N N 116.186 0.03 1 67 52 52 PHE H H 8.251 0.01 1 68 52 52 PHE N N 120.262 0.03 1 69 53 53 GLU H H 8.237 0.01 1 70 53 53 GLU N N 122.554 0.03 1 71 54 54 GLN H H 8.503 0.01 1 72 54 54 GLN N N 122.36 0.03 1 73 57 57 HIS H H 8.708 0.01 1 74 57 57 HIS N N 125.001 0.03 1 75 58 58 ILE H H 8.388 0.01 1 76 58 58 ILE N N 125.1 0.03 1 77 59 59 SER H H 8.58 0.01 1 78 59 59 SER N N 121.317 0.03 1 79 60 60 GLU H H 8.602 0.01 1 80 60 60 GLU N N 124.817 0.03 1 81 64 64 GLU H H 8.654 0.01 1 82 64 64 GLU N N 122.197 0.03 1 83 66 66 LYS H H 8.585 0.01 1 84 66 66 LYS N N 126.512 0.03 1 85 67 67 HIS H H 8.674 0.01 1 86 67 67 HIS N N 122.384 0.03 1 87 68 68 GLU H H 8.705 0.01 1 88 68 68 GLU N N 123.424 0.03 1 89 69 69 SER H H 8.736 0.01 1 90 69 69 SER N N 118.378 0.03 1 91 70 70 LEU H H 8.532 0.01 1 92 70 70 LEU N N 124.364 0.03 1 93 71 71 LEU H H 8.172 0.01 1 94 71 71 LEU N N 121.376 0.03 1 95 72 72 GLU H H 8.223 0.01 1 96 72 72 GLU N N 121.234 0.03 1 97 74 74 LEU H H 8.236 0.01 1 98 74 74 LEU N N 122.473 0.03 1 99 75 75 HIS H H 8.411 0.01 1 100 75 75 HIS N N 119.999 0.03 1 101 76 76 ARG H H 8.45 0.01 1 102 76 76 ARG N N 123.069 0.03 1 103 77 77 SER H H 8.629 0.01 1 104 77 77 SER N N 117.525 0.03 1 105 79 79 SER H H 8.446 0.01 1 106 79 79 SER N N 116.642 0.03 1 107 80 80 SER H H 8.548 0.01 1 108 80 80 SER N N 118.373 0.03 1 109 81 81 SER H H 8.479 0.01 1 110 81 81 SER N N 118.037 0.03 1 111 82 82 SER H H 8.51 0.01 1 112 82 82 SER N N 118.214 0.03 1 113 83 83 SER H H 8.542 0.01 1 114 83 83 SER N N 118.317 0.03 1 115 84 84 SER H H 8.536 0.01 1 116 84 84 SER N N 118.296 0.03 1 117 85 85 SER H H 8.537 0.01 1 118 85 85 SER N N 118.315 0.03 1 119 87 87 GLU H H 8.483 0.01 1 120 87 87 GLU N N 121.604 0.03 1 121 89 89 GLY H H 8.634 0.01 1 122 89 89 GLY N N 110.459 0.03 1 123 90 90 SER H H 8.472 0.01 1 124 90 90 SER N N 116.194 0.03 1 125 91 91 ASP H H 8.691 0.01 1 126 91 91 ASP N N 122.226 0.03 1 127 92 92 GLY H H 8.485 0.01 1 128 92 92 GLY N N 109.095 0.03 1 129 102 102 LYS H H 8.559 0.01 1 130 102 102 LYS N N 124.266 0.03 1 131 103 103 LYS H H 8.646 0.01 1 132 103 103 LYS N N 125.667 0.03 1 133 105 105 THR H H 8.58 0.01 1 134 105 105 THR N N 115.703 0.03 1 135 106 106 THR H H 8.289 0.01 1 136 106 106 THR N N 116.464 0.03 1 137 109 109 GLU H H 8.56 0.01 1 138 109 109 GLU N N 125.742 0.03 1 139 110 110 VAL H H 8.505 0.01 1 140 110 110 VAL N N 124.166 0.03 1 141 111 111 LYS H H 8.606 0.01 1 142 111 111 LYS N N 126.379 0.03 1 143 112 112 GLU H H 8.664 0.01 1 144 112 112 GLU N N 123.423 0.03 1 145 113 113 GLU H H 8.737 0.01 1 146 113 113 GLU N N 121.815 0.03 1 147 114 114 GLU H H 8.453 0.01 1 148 114 114 GLU N N 122.273 0.03 1 149 117 117 GLY H H 8.58 0.01 1 150 117 117 GLY N N 110.052 0.03 1 151 118 118 PHE H H 8.191 0.01 1 152 118 118 PHE N N 120.761 0.03 1 153 122 122 LEU H H 8.24 0.01 1 154 122 122 LEU N N 123.206 0.03 1 155 126 126 LEU H H 8.467 0.01 1 156 126 126 LEU N N 125.48 0.03 1 157 128 128 GLY H H 8.602 0.01 1 158 128 128 GLY N N 109.346 0.03 1 159 129 129 HIS H H 8.281 0.01 1 160 129 129 HIS N N 119.501 0.03 1 161 130 130 LYS H H 8.459 0.01 1 162 130 130 LYS N N 123.857 0.03 1 163 131 131 LYS H H 8.678 0.01 1 164 131 131 LYS N N 125.575 0.03 1 165 133 133 GLU H H 8.854 0.01 1 166 133 133 GLU N N 121.49 0.03 1 167 134 134 ASP H H 8.461 0.01 1 168 134 134 ASP N N 121.949 0.03 1 169 135 135 GLY H H 8.574 0.01 1 170 135 135 GLY N N 110.41 0.03 1 171 136 136 SER H H 8.334 0.01 1 172 136 136 SER N N 116.16 0.03 1 173 137 137 ALA H H 8.428 0.01 1 174 137 137 ALA N N 126.069 0.03 1 175 138 138 VAL H H 8.204 0.01 1 176 138 138 VAL N N 120.204 0.03 1 177 139 139 ALA H H 8.508 0.01 1 178 139 139 ALA N N 128.885 0.03 1 179 140 140 ALA H H 8.42 0.01 1 180 140 140 ALA N N 124.356 0.03 1 181 141 141 ALA H H 8.427 0.01 1 182 141 141 ALA N N 125.608 0.03 1 183 144 144 VAL H H 8.528 0.01 1 184 144 144 VAL N N 127.011 0.03 1 185 145 145 VAL H H 8.63 0.01 1 186 145 145 VAL N N 128.935 0.03 1 187 149 149 VAL H H 8.418 0.01 1 188 149 149 VAL N N 121.418 0.03 1 189 150 150 GLU H H 8.661 0.01 1 190 150 150 GLU N N 125.706 0.03 1 191 153 153 HIS H H 8.578 0.01 1 192 153 153 HIS N N 119.658 0.03 1 193 155 155 VAL H H 8.548 0.01 1 194 155 155 VAL N N 121.649 0.03 1 195 156 156 GLU H H 8.682 0.01 1 196 156 156 GLU N N 125.86 0.03 1 197 159 159 GLY H H 8.652 0.01 1 198 159 159 GLY N N 110.636 0.03 1 199 160 160 ILE H H 8.165 0.01 1 200 160 160 ILE N N 120.562 0.03 1 201 168 168 LEU H H 8.399 0.01 1 202 168 168 LEU N N 125.425 0.03 1 203 170 170 GLY H H 8.692 0.01 1 204 170 170 GLY N N 109.843 0.03 1 205 171 171 TYR H H 8.044 0.01 1 206 171 171 TYR N N 120.847 0.03 1 207 172 172 HIS H H 8.288 0.01 1 208 172 172 HIS N N 125.109 0.03 1 209 174 174 LYS H H 8.723 0.01 1 210 174 174 LYS N N 122.297 0.03 1 211 175 175 THR H H 8.435 0.01 1 212 175 175 THR N N 116.429 0.03 1 213 176 176 THR H H 8.396 0.01 1 214 176 176 THR N N 117.544 0.03 1 215 178 178 GLU H H 8.622 0.01 1 216 178 178 GLU N N 125.149 0.03 1 217 183 183 ASP H H 8.523 0.01 1 218 183 183 ASP N N 121.738 0.03 1 219 184 184 LYS H H 8.329 0.01 1 220 184 184 LYS N N 121.406 0.03 1 221 185 185 GLU H H 8.19 0.01 1 222 185 185 GLU N N 127.65 0.03 1 stop_ save_ save_Assignment_277K_7.42pH _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CARA stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $277K-7.42pH _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name ERD14 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 4 4 GLU H H 8.785 0.01 1 2 4 4 GLU N N 123.873 0.03 1 3 5 5 ILE H H 8.569 0.01 1 4 5 5 ILE N N 124.646 0.03 1 5 6 6 LYS H H 8.647 0.01 1 6 6 6 LYS N N 127.088 0.03 1 7 7 7 ASN H H 8.666 0.01 1 8 7 7 ASN N N 121.143 0.03 1 9 10 10 GLU H H 8.706 0.01 1 10 10 10 GLU N N 121.62 0.03 1 11 11 11 GLN H H 8.55 0.01 1 12 11 11 GLN N N 121.572 0.03 1 13 12 12 GLU H H 8.614 0.01 1 14 12 12 GLU N N 123.505 0.03 1 15 13 13 VAL H H 8.486 0.01 1 16 13 13 VAL N N 124.661 0.03 1 17 15 15 LYS H H 8.62 0.01 1 18 15 15 LYS N N 123.07 0.03 1 19 17 17 ALA H H 8.669 0.01 1 20 17 17 ALA N N 129.238 0.03 1 21 18 18 THR H H 8.353 0.01 1 22 18 18 THR N N 114.651 0.03 1 23 19 19 GLU H H 8.645 0.01 1 24 19 19 GLU N N 123.644 0.03 1 25 20 20 GLU H H 8.654 0.01 1 26 20 20 GLU N N 122.824 0.03 1 27 21 21 SER H H 8.588 0.01 1 28 21 21 SER N N 117.724 0.03 1 29 22 22 SER H H 8.559 0.01 1 30 22 22 SER N N 118.606 0.03 1 31 23 23 ALA H H 8.443 0.01 1 32 23 23 ALA N N 126.304 0.03 1 33 24 24 GLU H H 8.435 0.01 1 34 24 24 GLU N N 120.455 0.03 1 35 26 26 THR H H 8.396 0.01 1 36 26 26 THR N N 118.598 0.03 1 37 27 27 ASP H H 8.48 0.01 1 38 27 27 ASP N N 123.253 0.03 1 39 29 29 GLY H H 8.643 0.01 1 40 29 29 GLY N N 109.512 0.03 1 41 30 30 LEU H H 8.053 0.01 1 42 30 30 LEU N N 121.199 0.03 1 43 31 31 PHE H H 8.241 0.01 1 44 31 31 PHE N N 119.644 0.03 1 45 33 33 PHE H H 8.202 0.01 1 46 33 33 PHE N N 120.548 0.03 1 47 35 35 GLY H H 8.093 0.01 1 48 35 35 GLY N N 109.101 0.03 1 49 36 36 LYS H H 8.165 0.01 1 50 36 36 LYS N N 121.289 0.03 1 51 37 37 LYS H H 8.604 0.01 1 52 37 37 LYS N N 123.703 0.03 1 53 38 38 LYS H H 8.583 0.01 1 54 38 38 LYS N N 124.001 0.03 1 55 39 39 ASP H H 8.56 0.01 1 56 39 39 ASP N N 122.335 0.03 1 57 41 41 THR H H 8.455 0.01 1 58 41 41 THR N N 116.425 0.03 1 59 44 44 GLU H H 8.761 0.01 1 60 44 44 GLU N N 121.575 0.03 1 61 46 46 THR H H 8.507 0.01 1 62 46 46 THR N N 119.791 0.03 1 63 49 49 ALA H H 8.604 0.01 1 64 49 49 ALA N N 129.468 0.03 1 65 50 50 SER H H 8.512 0.01 1 66 50 50 SER N N 116.186 0.03 1 67 52 52 PHE H H 8.251 0.01 1 68 52 52 PHE N N 120.27 0.03 1 69 53 53 GLU H H 8.236 0.01 1 70 53 53 GLU N N 122.578 0.03 1 71 54 54 GLN H H 8.504 0.01 1 72 54 54 GLN N N 122.384 0.03 1 73 57 57 HIS H H 8.691 0.01 1 74 57 57 HIS N N 125.144 0.03 1 75 58 58 ILE H H 8.368 0.01 1 76 58 58 ILE N N 125.1 0.03 1 77 59 59 SER H H 8.576 0.01 1 78 59 59 SER N N 121.317 0.03 1 79 60 60 GLU H H 8.602 0.01 1 80 60 60 GLU N N 124.814 0.03 1 81 64 64 GLU H H 8.654 0.01 1 82 64 64 GLU N N 122.197 0.03 1 83 66 66 LYS H H 8.585 0.01 1 84 66 66 LYS N N 126.54 0.03 1 85 67 67 HIS H H 8.646 0.01 1 86 67 67 HIS N N 122.544 0.03 1 87 68 68 GLU H H 8.696 0.01 1 88 68 68 GLU N N 123.424 0.03 1 89 69 69 SER H H 8.73 0.01 1 90 69 69 SER N N 118.325 0.03 1 91 70 70 LEU H H 8.517 0.01 1 92 70 70 LEU N N 124.342 0.03 1 93 71 71 LEU H H 8.165 0.01 1 94 71 71 LEU N N 121.403 0.03 1 95 72 72 GLU H H 8.225 0.01 1 96 72 72 GLU N N 121.234 0.03 1 97 74 74 LEU H H 8.237 0.01 1 98 74 74 LEU N N 122.505 0.03 1 99 75 75 HIS H H 8.396 0.01 1 100 75 75 HIS N N 120.148 0.03 1 101 77 77 SER H H 8.628 0.01 1 102 77 77 SER N N 117.559 0.03 1 103 79 79 SER H H 8.446 0.01 1 104 79 79 SER N N 116.652 0.03 1 105 80 80 SER H H 8.55 0.01 1 106 80 80 SER N N 118.39 0.03 1 107 81 81 SER H H 8.479 0.01 1 108 81 81 SER N N 118.042 0.03 1 109 82 82 SER H H 8.513 0.01 1 110 82 82 SER N N 118.223 0.03 1 111 83 83 SER H H 8.542 0.01 1 112 83 83 SER N N 118.327 0.03 1 113 84 84 SER H H 8.538 0.01 1 114 84 84 SER N N 118.305 0.03 1 115 85 85 SER H H 8.537 0.01 1 116 85 85 SER N N 118.322 0.03 1 117 87 87 GLU H H 8.483 0.01 1 118 87 87 GLU N N 121.611 0.03 1 119 89 89 GLY H H 8.634 0.01 1 120 89 89 GLY N N 110.459 0.03 1 121 90 90 SER H H 8.473 0.01 1 122 90 90 SER N N 116.199 0.03 1 123 91 91 ASP H H 8.691 0.01 1 124 91 91 ASP N N 122.226 0.03 1 125 92 92 GLY H H 8.485 0.01 1 126 92 92 GLY N N 109.1 0.03 1 127 102 102 LYS H H 8.559 0.01 1 128 102 102 LYS N N 124.266 0.03 1 129 103 103 LYS H H 8.648 0.01 1 130 103 103 LYS N N 125.659 0.03 1 131 105 105 THR H H 8.58 0.01 1 132 105 105 THR N N 115.708 0.03 1 133 106 106 THR H H 8.289 0.01 1 134 106 106 THR N N 116.474 0.03 1 135 109 109 GLU H H 8.56 0.01 1 136 109 109 GLU N N 125.75 0.03 1 137 110 110 VAL H H 8.504 0.01 1 138 110 110 VAL N N 124.17 0.03 1 139 111 111 LYS H H 8.606 0.01 1 140 111 111 LYS N N 126.379 0.03 1 141 112 112 GLU H H 8.664 0.01 1 142 112 112 GLU N N 123.423 0.03 1 143 113 113 GLU H H 8.737 0.01 1 144 113 113 GLU N N 121.822 0.03 1 145 114 114 GLU H H 8.453 0.01 1 146 114 114 GLU N N 122.273 0.03 1 147 117 117 GLY H H 8.58 0.01 1 148 117 117 GLY N N 110.052 0.03 1 149 118 118 PHE H H 8.191 0.01 1 150 118 118 PHE N N 120.761 0.03 1 151 122 122 LEU H H 8.24 0.01 1 152 122 122 LEU N N 123.208 0.03 1 153 126 126 LEU H H 8.466 0.01 1 154 126 126 LEU N N 125.48 0.03 1 155 128 128 GLY H H 8.602 0.01 1 156 128 128 GLY N N 109.346 0.03 1 157 129 129 HIS H H 8.257 0.01 1 158 129 129 HIS N N 119.606 0.03 1 159 130 130 LYS H H 8.437 0.01 1 160 130 130 LYS N N 123.855 0.03 1 161 131 131 LYS H H 8.674 0.01 1 162 131 131 LYS N N 125.575 0.03 1 163 133 133 GLU H H 8.854 0.01 1 164 133 133 GLU N N 121.504 0.03 1 165 134 134 ASP H H 8.459 0.01 1 166 134 134 ASP N N 121.949 0.03 1 167 135 135 GLY H H 8.574 0.01 1 168 135 135 GLY N N 110.41 0.03 1 169 136 136 SER H H 8.334 0.01 1 170 136 136 SER N N 116.16 0.03 1 171 137 137 ALA H H 8.428 0.01 1 172 137 137 ALA N N 126.069 0.03 1 173 138 138 VAL H H 8.203 0.01 1 174 138 138 VAL N N 120.208 0.03 1 175 139 139 ALA H H 8.507 0.01 1 176 139 139 ALA N N 128.887 0.03 1 177 140 140 ALA H H 8.419 0.01 1 178 140 140 ALA N N 124.356 0.03 1 179 141 141 ALA H H 8.427 0.01 1 180 141 141 ALA N N 125.608 0.03 1 181 144 144 VAL H H 8.527 0.01 1 182 144 144 VAL N N 127.019 0.03 1 183 145 145 VAL H H 8.63 0.01 1 184 145 145 VAL N N 128.941 0.03 1 185 149 149 VAL H H 8.416 0.01 1 186 149 149 VAL N N 121.415 0.03 1 187 150 150 GLU H H 8.661 0.01 1 188 150 150 GLU N N 125.695 0.03 1 189 153 153 HIS H H 8.555 0.01 1 190 153 153 HIS N N 119.877 0.03 1 191 155 155 VAL H H 8.547 0.01 1 192 155 155 VAL N N 121.633 0.03 1 193 156 156 GLU H H 8.678 0.01 1 194 156 156 GLU N N 125.798 0.03 1 195 159 159 GLY H H 8.65 0.01 1 196 159 159 GLY N N 110.631 0.03 1 197 160 160 ILE H H 8.164 0.01 1 198 160 160 ILE N N 120.566 0.03 1 199 168 168 LEU H H 8.403 0.01 1 200 168 168 LEU N N 125.443 0.03 1 201 170 170 GLY H H 8.687 0.01 1 202 170 170 GLY N N 109.838 0.03 1 203 171 171 TYR H H 8.039 0.01 1 204 171 171 TYR N N 120.824 0.03 1 205 172 172 HIS H H 8.276 0.01 1 206 172 172 HIS N N 125.193 0.03 1 207 174 174 LYS H H 8.727 0.01 1 208 174 174 LYS N N 122.273 0.03 1 209 175 175 THR H H 8.435 0.01 1 210 175 175 THR N N 116.429 0.03 1 211 176 176 THR H H 8.396 0.01 1 212 176 176 THR N N 117.563 0.03 1 213 178 178 GLU H H 8.622 0.01 1 214 178 178 GLU N N 125.169 0.03 1 215 183 183 ASP H H 8.523 0.01 1 216 183 183 ASP N N 121.741 0.03 1 217 184 184 LYS H H 8.328 0.01 1 218 184 184 LYS N N 121.406 0.03 1 219 185 185 GLU H H 8.19 0.01 1 220 185 185 GLU N N 127.652 0.03 1 stop_ save_ save_Assignment_277K_7.57pH _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CARA stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $277K-7.57pH _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name ERD14 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 4 4 GLU H H 8.783 0.01 1 2 4 4 GLU N N 123.867 0.03 1 3 5 5 ILE H H 8.568 0.01 1 4 5 5 ILE N N 124.644 0.03 1 5 6 6 LYS H H 8.647 0.01 1 6 6 6 LYS N N 127.088 0.03 1 7 7 7 ASN H H 8.663 0.01 1 8 7 7 ASN N N 121.143 0.03 1 9 10 10 GLU H H 8.703 0.01 1 10 10 10 GLU N N 121.616 0.03 1 11 11 11 GLN H H 8.55 0.01 1 12 11 11 GLN N N 121.572 0.03 1 13 12 12 GLU H H 8.614 0.01 1 14 12 12 GLU N N 123.505 0.03 1 15 13 13 VAL H H 8.485 0.01 1 16 13 13 VAL N N 124.663 0.03 1 17 15 15 LYS H H 8.62 0.01 1 18 15 15 LYS N N 123.07 0.03 1 19 17 17 ALA H H 8.669 0.01 1 20 17 17 ALA N N 129.24 0.03 1 21 18 18 THR H H 8.351 0.01 1 22 18 18 THR N N 114.651 0.03 1 23 19 19 GLU H H 8.645 0.01 1 24 19 19 GLU N N 123.642 0.03 1 25 20 20 GLU H H 8.653 0.01 1 26 20 20 GLU N N 122.828 0.03 1 27 21 21 SER H H 8.585 0.01 1 28 21 21 SER N N 117.724 0.03 1 29 22 22 SER H H 8.555 0.01 1 30 22 22 SER N N 118.606 0.03 1 31 23 23 ALA H H 8.443 0.01 1 32 23 23 ALA N N 126.307 0.03 1 33 24 24 GLU H H 8.434 0.01 1 34 24 24 GLU N N 120.455 0.03 1 35 26 26 THR H H 8.395 0.01 1 36 26 26 THR N N 118.608 0.03 1 37 27 27 ASP H H 8.478 0.01 1 38 27 27 ASP N N 123.255 0.03 1 39 29 29 GLY H H 8.639 0.01 1 40 29 29 GLY N N 109.507 0.03 1 41 30 30 LEU H H 8.05 0.01 1 42 30 30 LEU N N 121.194 0.03 1 43 31 31 PHE H H 8.241 0.01 1 44 31 31 PHE N N 119.644 0.03 1 45 33 33 PHE H H 8.201 0.01 1 46 33 33 PHE N N 120.548 0.03 1 47 35 35 GLY H H 8.091 0.01 1 48 35 35 GLY N N 109.096 0.03 1 49 36 36 LYS H H 8.164 0.01 1 50 36 36 LYS N N 121.294 0.03 1 51 37 37 LYS H H 8.604 0.01 1 52 37 37 LYS N N 123.683 0.03 1 53 38 38 LYS H H 8.583 0.01 1 54 38 38 LYS N N 124.001 0.03 1 55 39 39 ASP H H 8.56 0.01 1 56 39 39 ASP N N 122.343 0.03 1 57 41 41 THR H H 8.455 0.01 1 58 41 41 THR N N 116.425 0.03 1 59 44 44 GLU H H 8.759 0.01 1 60 44 44 GLU N N 121.572 0.03 1 61 46 46 THR H H 8.505 0.01 1 62 46 46 THR N N 119.791 0.03 1 63 49 49 ALA H H 8.604 0.01 1 64 49 49 ALA N N 129.472 0.03 1 65 50 50 SER H H 8.509 0.01 1 66 50 50 SER N N 116.186 0.03 1 67 52 52 PHE H H 8.249 0.01 1 68 52 52 PHE N N 120.281 0.03 1 69 53 53 GLU H H 8.237 0.01 1 70 53 53 GLU N N 122.598 0.03 1 71 54 54 GLN H H 8.505 0.01 1 72 54 54 GLN N N 122.421 0.03 1 73 57 57 HIS H H 8.673 0.01 1 74 57 57 HIS N N 125.254 0.03 1 75 58 58 ILE H H 8.348 0.01 1 76 58 58 ILE N N 125.098 0.03 1 77 59 59 SER H H 8.569 0.01 1 78 59 59 SER N N 121.32 0.03 1 79 60 60 GLU H H 8.6 0.01 1 80 60 60 GLU N N 124.813 0.03 1 81 64 64 GLU H H 8.652 0.01 1 82 64 64 GLU N N 122.194 0.03 1 83 66 66 LYS H H 8.586 0.01 1 84 66 66 LYS N N 126.577 0.03 1 85 67 67 HIS H H 8.624 0.01 1 86 67 67 HIS N N 122.666 0.03 1 87 68 68 GLU H H 8.686 0.01 1 88 68 68 GLU N N 123.418 0.03 1 89 69 69 SER H H 8.723 0.01 1 90 69 69 SER N N 118.291 0.03 1 91 70 70 LEU H H 8.509 0.01 1 92 70 70 LEU N N 124.323 0.03 1 93 71 71 LEU H H 8.158 0.01 1 94 71 71 LEU N N 121.419 0.03 1 95 72 72 GLU H H 8.226 0.01 1 96 72 72 GLU N N 121.229 0.03 1 97 74 74 LEU H H 8.24 0.01 1 98 74 74 LEU N N 122.55 0.03 1 99 75 75 HIS H H 8.381 0.01 1 100 75 75 HIS N N 120.272 0.03 1 101 77 77 SER H H 8.623 0.01 1 102 77 77 SER N N 117.598 0.03 1 103 79 79 SER H H 8.442 0.01 1 104 79 79 SER N N 116.661 0.03 1 105 80 80 SER H H 8.551 0.01 1 106 80 80 SER N N 118.404 0.03 1 107 81 81 SER H H 8.475 0.01 1 108 81 81 SER N N 118.047 0.03 1 109 82 82 SER H H 8.514 0.01 1 110 82 82 SER N N 118.227 0.03 1 111 83 83 SER H H 8.541 0.01 1 112 83 83 SER N N 118.336 0.03 1 113 84 84 SER H H 8.538 0.01 1 114 84 84 SER N N 118.316 0.03 1 115 85 85 SER H H 8.536 0.01 1 116 85 85 SER N N 118.334 0.03 1 117 87 87 GLU H H 8.481 0.01 1 118 87 87 GLU N N 121.607 0.03 1 119 89 89 GLY H H 8.631 0.01 1 120 89 89 GLY N N 110.47 0.03 1 121 90 90 SER H H 8.471 0.01 1 122 90 90 SER N N 116.194 0.03 1 123 91 91 ASP H H 8.691 0.01 1 124 91 91 ASP N N 122.226 0.03 1 125 92 92 GLY H H 8.482 0.01 1 126 92 92 GLY N N 109.095 0.03 1 127 102 102 LYS H H 8.559 0.01 1 128 102 102 LYS N N 124.266 0.03 1 129 103 103 LYS H H 8.648 0.01 1 130 103 103 LYS N N 125.655 0.03 1 131 105 105 THR H H 8.579 0.01 1 132 105 105 THR N N 115.708 0.03 1 133 106 106 THR H H 8.288 0.01 1 134 106 106 THR N N 116.479 0.03 1 135 109 109 GLU H H 8.56 0.01 1 136 109 109 GLU N N 125.751 0.03 1 137 110 110 VAL H H 8.504 0.01 1 138 110 110 VAL N N 124.174 0.03 1 139 111 111 LYS H H 8.606 0.01 1 140 111 111 LYS N N 126.379 0.03 1 141 112 112 GLU H H 8.664 0.01 1 142 112 112 GLU N N 123.423 0.03 1 143 113 113 GLU H H 8.734 0.01 1 144 113 113 GLU N N 121.829 0.03 1 145 114 114 GLU H H 8.453 0.01 1 146 114 114 GLU N N 122.273 0.03 1 147 117 117 GLY H H 8.577 0.01 1 148 117 117 GLY N N 110.063 0.03 1 149 118 118 PHE H H 8.19 0.01 1 150 118 118 PHE N N 120.761 0.03 1 151 122 122 LEU H H 8.239 0.01 1 152 122 122 LEU N N 123.21 0.03 1 153 126 126 LEU H H 8.465 0.01 1 154 126 126 LEU N N 125.48 0.03 1 155 128 128 GLY H H 8.599 0.01 1 156 128 128 GLY N N 109.346 0.03 1 157 129 129 HIS H H 8.226 0.01 1 158 129 129 HIS N N 119.695 0.03 1 159 130 130 LYS H H 8.414 0.01 1 160 130 130 LYS N N 123.853 0.03 1 161 131 131 LYS H H 8.668 0.01 1 162 131 131 LYS N N 125.58 0.03 1 163 133 133 GLU H H 8.851 0.01 1 164 133 133 GLU N N 121.5 0.03 1 165 134 134 ASP H H 8.458 0.01 1 166 134 134 ASP N N 121.946 0.03 1 167 135 135 GLY H H 8.572 0.01 1 168 135 135 GLY N N 110.41 0.03 1 169 136 136 SER H H 8.332 0.01 1 170 136 136 SER N N 116.16 0.03 1 171 137 137 ALA H H 8.428 0.01 1 172 137 137 ALA N N 126.069 0.03 1 173 138 138 VAL H H 8.202 0.01 1 174 138 138 VAL N N 120.212 0.03 1 175 139 139 ALA H H 8.507 0.01 1 176 139 139 ALA N N 128.886 0.03 1 177 140 140 ALA H H 8.418 0.01 1 178 140 140 ALA N N 124.358 0.03 1 179 141 141 ALA H H 8.427 0.01 1 180 141 141 ALA N N 125.608 0.03 1 181 144 144 VAL H H 8.527 0.01 1 182 144 144 VAL N N 127.02 0.03 1 183 145 145 VAL H H 8.63 0.01 1 184 145 145 VAL N N 128.941 0.03 1 185 149 149 VAL H H 8.414 0.01 1 186 149 149 VAL N N 121.413 0.03 1 187 150 150 GLU H H 8.661 0.01 1 188 150 150 GLU N N 125.687 0.03 1 189 153 153 HIS H H 8.532 0.01 1 190 153 153 HIS N N 120.082 0.03 1 191 155 155 VAL H H 8.545 0.01 1 192 155 155 VAL N N 121.618 0.03 1 193 156 156 GLU H H 8.674 0.01 1 194 156 156 GLU N N 125.755 0.03 1 195 159 159 GLY H H 8.645 0.01 1 196 159 159 GLY N N 110.62 0.03 1 197 160 160 ILE H H 8.161 0.01 1 198 160 160 ILE N N 120.566 0.03 1 199 168 168 LEU H H 8.406 0.01 1 200 168 168 LEU N N 125.463 0.03 1 201 170 170 GLY H H 8.678 0.01 1 202 170 170 GLY N N 109.822 0.03 1 203 171 171 TYR H H 8.032 0.01 1 204 171 171 TYR N N 120.783 0.03 1 205 172 172 HIS H H 8.261 0.01 1 206 172 172 HIS N N 125.266 0.03 1 207 174 174 LYS H H 8.729 0.01 1 208 174 174 LYS N N 122.259 0.03 1 209 175 175 THR H H 8.435 0.01 1 210 175 175 THR N N 116.429 0.03 1 211 176 176 THR H H 8.392 0.01 1 212 176 176 THR N N 117.583 0.03 1 213 178 178 GLU H H 8.623 0.01 1 214 178 178 GLU N N 125.191 0.03 1 215 183 183 ASP H H 8.52 0.01 1 216 183 183 ASP N N 121.745 0.03 1 217 184 184 LYS H H 8.326 0.01 1 218 184 184 LYS N N 121.406 0.03 1 219 185 185 GLU H H 8.19 0.01 1 220 185 185 GLU N N 127.654 0.03 1 stop_ save_ save_Assignment_277K_7.70pH _Saveframe_category assigned_chemical_shifts _Details . loop_ _Software_label $CARA stop_ loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $277K-7.70pH _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name ERD14 _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 4 4 GLU H H 8.783 0.01 1 2 4 4 GLU N N 123.867 0.03 1 3 5 5 ILE H H 8.567 0.01 1 4 5 5 ILE N N 124.644 0.03 1 5 6 6 LYS H H 8.646 0.01 1 6 6 6 LYS N N 127.089 0.03 1 7 7 7 ASN H H 8.665 0.01 1 8 7 7 ASN N N 121.14 0.03 1 9 10 10 GLU H H 8.706 0.01 1 10 10 10 GLU N N 121.62 0.03 1 11 11 11 GLN H H 8.55 0.01 1 12 11 11 GLN N N 121.572 0.03 1 13 12 12 GLU H H 8.614 0.01 1 14 12 12 GLU N N 123.505 0.03 1 15 13 13 VAL H H 8.485 0.01 1 16 13 13 VAL N N 124.669 0.03 1 17 15 15 LYS H H 8.62 0.01 1 18 15 15 LYS N N 123.07 0.03 1 19 17 17 ALA H H 8.669 0.01 1 20 17 17 ALA N N 129.242 0.03 1 21 18 18 THR H H 8.353 0.01 1 22 18 18 THR N N 114.656 0.03 1 23 19 19 GLU H H 8.645 0.01 1 24 19 19 GLU N N 123.642 0.03 1 25 20 20 GLU H H 8.653 0.01 1 26 20 20 GLU N N 122.833 0.03 1 27 21 21 SER H H 8.588 0.01 1 28 21 21 SER N N 117.729 0.03 1 29 22 22 SER H H 8.558 0.01 1 30 22 22 SER N N 118.611 0.03 1 31 23 23 ALA H H 8.443 0.01 1 32 23 23 ALA N N 126.311 0.03 1 33 24 24 GLU H H 8.435 0.01 1 34 24 24 GLU N N 120.459 0.03 1 35 26 26 THR H H 8.397 0.01 1 36 26 26 THR N N 118.613 0.03 1 37 27 27 ASP H H 8.479 0.01 1 38 27 27 ASP N N 123.26 0.03 1 39 29 29 GLY H H 8.641 0.01 1 40 29 29 GLY N N 109.507 0.03 1 41 30 30 LEU H H 8.053 0.01 1 42 30 30 LEU N N 121.197 0.03 1 43 31 31 PHE H H 8.241 0.01 1 44 31 31 PHE N N 119.644 0.03 1 45 33 33 PHE H H 8.202 0.01 1 46 33 33 PHE N N 120.552 0.03 1 47 35 35 GLY H H 8.092 0.01 1 48 35 35 GLY N N 109.101 0.03 1 49 36 36 LYS H H 8.165 0.01 1 50 36 36 LYS N N 121.3 0.03 1 51 37 37 LYS H H 8.603 0.01 1 52 37 37 LYS N N 123.666 0.03 1 53 38 38 LYS H H 8.583 0.01 1 54 38 38 LYS N N 124.001 0.03 1 55 39 39 ASP H H 8.56 0.01 1 56 39 39 ASP N N 122.342 0.03 1 57 41 41 THR H H 8.455 0.01 1 58 41 41 THR N N 116.425 0.03 1 59 44 44 GLU H H 8.76 0.01 1 60 44 44 GLU N N 121.579 0.03 1 61 46 46 THR H H 8.507 0.01 1 62 46 46 THR N N 119.795 0.03 1 63 49 49 ALA H H 8.604 0.01 1 64 49 49 ALA N N 129.481 0.03 1 65 50 50 SER H H 8.512 0.01 1 66 50 50 SER N N 116.191 0.03 1 67 52 52 PHE H H 8.251 0.01 1 68 52 52 PHE N N 120.289 0.03 1 69 53 53 GLU H H 8.238 0.01 1 70 53 53 GLU N N 122.624 0.03 1 71 54 54 GLN H H 8.507 0.01 1 72 54 54 GLN N N 122.452 0.03 1 73 57 57 HIS H H 8.663 0.01 1 74 57 57 HIS N N 125.362 0.03 1 75 58 58 ILE H H 8.335 0.01 1 76 58 58 ILE N N 125.096 0.03 1 77 59 59 SER H H 8.565 0.01 1 78 59 59 SER N N 121.331 0.03 1 79 60 60 GLU H H 8.601 0.01 1 80 60 60 GLU N N 124.813 0.03 1 81 64 64 GLU H H 8.654 0.01 1 82 64 64 GLU N N 122.194 0.03 1 83 66 66 LYS H H 8.586 0.01 1 84 66 66 LYS N N 126.607 0.03 1 85 67 67 HIS H H 8.61 0.01 1 86 67 67 HIS N N 122.756 0.03 1 87 68 68 GLU H H 8.679 0.01 1 88 68 68 GLU N N 123.418 0.03 1 89 69 69 SER H H 8.721 0.01 1 90 69 69 SER N N 118.267 0.03 1 91 70 70 LEU H H 8.504 0.01 1 92 70 70 LEU N N 124.31 0.03 1 93 71 71 LEU H H 8.156 0.01 1 94 71 71 LEU N N 121.441 0.03 1 95 72 72 GLU H H 8.231 0.01 1 96 72 72 GLU N N 121.226 0.03 1 97 74 74 LEU H H 8.241 0.01 1 98 74 74 LEU N N 122.582 0.03 1 99 75 75 HIS H H 8.372 0.01 1 100 75 75 HIS N N 120.38 0.03 1 101 77 77 SER H H 8.625 0.01 1 102 77 77 SER N N 117.665 0.03 1 103 79 79 SER H H 8.446 0.01 1 104 79 79 SER N N 116.666 0.03 1 105 80 80 SER H H 8.552 0.01 1 106 80 80 SER N N 118.411 0.03 1 107 81 81 SER H H 8.483 0.01 1 108 81 81 SER N N 118.09 0.03 1 109 82 82 SER H H 8.515 0.01 1 110 82 82 SER N N 118.232 0.03 1 111 83 83 SER H H 8.541 0.01 1 112 83 83 SER N N 118.339 0.03 1 113 84 84 SER H H 8.538 0.01 1 114 84 84 SER N N 118.313 0.03 1 115 85 85 SER H H 8.536 0.01 1 116 85 85 SER N N 118.329 0.03 1 117 87 87 GLU H H 8.483 0.01 1 118 87 87 GLU N N 121.621 0.03 1 119 89 89 GLY H H 8.634 0.01 1 120 89 89 GLY N N 110.475 0.03 1 121 90 90 SER H H 8.472 0.01 1 122 90 90 SER N N 116.199 0.03 1 123 91 91 ASP H H 8.691 0.01 1 124 91 91 ASP N N 122.226 0.03 1 125 92 92 GLY H H 8.484 0.01 1 126 92 92 GLY N N 109.1 0.03 1 127 102 102 LYS H H 8.559 0.01 1 128 102 102 LYS N N 124.266 0.03 1 129 103 103 LYS H H 8.649 0.01 1 130 103 103 LYS N N 125.645 0.03 1 131 105 105 THR H H 8.581 0.01 1 132 105 105 THR N N 115.713 0.03 1 133 106 106 THR H H 8.29 0.01 1 134 106 106 THR N N 116.488 0.03 1 135 109 109 GLU H H 8.56 0.01 1 136 109 109 GLU N N 125.753 0.03 1 137 110 110 VAL H H 8.506 0.01 1 138 110 110 VAL N N 124.181 0.03 1 139 111 111 LYS H H 8.606 0.01 1 140 111 111 LYS N N 126.379 0.03 1 141 112 112 GLU H H 8.664 0.01 1 142 112 112 GLU N N 123.423 0.03 1 143 113 113 GLU H H 8.737 0.01 1 144 113 113 GLU N N 121.829 0.03 1 145 114 114 GLU H H 8.456 0.01 1 146 114 114 GLU N N 122.273 0.03 1 147 117 117 GLY H H 8.581 0.01 1 148 117 117 GLY N N 110.068 0.03 1 149 118 118 PHE H H 8.192 0.01 1 150 118 118 PHE N N 120.769 0.03 1 151 122 122 LEU H H 8.239 0.01 1 152 122 122 LEU N N 123.221 0.03 1 153 126 126 LEU H H 8.464 0.01 1 154 126 126 LEU N N 125.48 0.03 1 155 128 128 GLY H H 8.601 0.01 1 156 128 128 GLY N N 109.351 0.03 1 157 129 129 HIS H H 8.208 0.01 1 158 129 129 HIS N N 119.757 0.03 1 159 130 130 LYS H H 8.4 0.01 1 160 130 130 LYS N N 123.857 0.03 1 161 131 131 LYS H H 8.662 0.01 1 162 131 131 LYS N N 125.584 0.03 1 163 133 133 GLU H H 8.852 0.01 1 164 133 133 GLU N N 121.507 0.03 1 165 134 134 ASP H H 8.46 0.01 1 166 134 134 ASP N N 121.949 0.03 1 167 135 135 GLY H H 8.573 0.01 1 168 135 135 GLY N N 110.415 0.03 1 169 136 136 SER H H 8.333 0.01 1 170 136 136 SER N N 116.16 0.03 1 171 137 137 ALA H H 8.428 0.01 1 172 137 137 ALA N N 126.069 0.03 1 173 138 138 VAL H H 8.203 0.01 1 174 138 138 VAL N N 120.208 0.03 1 175 139 139 ALA H H 8.506 0.01 1 176 139 139 ALA N N 128.889 0.03 1 177 140 140 ALA H H 8.418 0.01 1 178 140 140 ALA N N 124.358 0.03 1 179 141 141 ALA H H 8.427 0.01 1 180 141 141 ALA N N 125.608 0.03 1 181 144 144 VAL H H 8.527 0.01 1 182 144 144 VAL N N 127.021 0.03 1 183 145 145 VAL H H 8.63 0.01 1 184 145 145 VAL N N 128.944 0.03 1 185 149 149 VAL H H 8.416 0.01 1 186 149 149 VAL N N 121.415 0.03 1 187 150 150 GLU H H 8.662 0.01 1 188 150 150 GLU N N 125.683 0.03 1 189 153 153 HIS H H 8.516 0.01 1 190 153 153 HIS N N 120.264 0.03 1 191 155 155 VAL H H 8.544 0.01 1 192 155 155 VAL N N 121.599 0.03 1 193 156 156 GLU H H 8.671 0.01 1 194 156 156 GLU N N 125.729 0.03 1 195 159 159 GLY H H 8.647 0.01 1 196 159 159 GLY N N 110.615 0.03 1 197 160 160 ILE H H 8.163 0.01 1 198 160 160 ILE N N 120.562 0.03 1 199 168 168 LEU H H 8.411 0.01 1 200 168 168 LEU N N 125.485 0.03 1 201 170 170 GLY H H 8.677 0.01 1 202 170 170 GLY N N 109.811 0.03 1 203 171 171 TYR H H 8.03 0.01 1 204 171 171 TYR N N 120.756 0.03 1 205 172 172 HIS H H 8.254 0.01 1 206 172 172 HIS N N 125.329 0.03 1 207 174 174 LYS H H 8.735 0.01 1 208 174 174 LYS N N 122.248 0.03 1 209 175 175 THR H H 8.435 0.01 1 210 175 175 THR N N 116.429 0.03 1 211 176 176 THR H H 8.394 0.01 1 212 176 176 THR N N 117.597 0.03 1 213 178 178 GLU H H 8.623 0.01 1 214 178 178 GLU N N 125.2 0.03 1 215 183 183 ASP H H 8.523 0.01 1 216 183 183 ASP N N 121.755 0.03 1 217 184 184 LYS H H 8.329 0.01 1 218 184 184 LYS N N 121.42 0.03 1 219 185 185 GLU H H 8.19 0.01 1 220 185 185 GLU N N 127.656 0.03 1 stop_ save_