data_26505 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; 13C NMR Relaxation Studies of RNA Base and Ribose Nuclei Reveal a Complex Pattern of Motions in the RNA Binding Site for Human U1A Protein ; _BMRB_accession_number 26505 _BMRB_flat_file_name bmr26505.str _Entry_type original _Submission_date 2015-01-22 _Accession_date 2015-01-22 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Shajani Zahra . . 2 Varani Gabriele . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count heteronucl_NOE 2 T1_relaxation 2 T1rho_relaxation 2 S2_parameters 2 stop_ loop_ _Data_type _Data_type_count "T1 relaxation values" 98 "T1rho relaxation values" 98 "order parameters" 65 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2015-02-11 original author . stop_ _Original_release_date 2015-02-11 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title '13C NMR Relaxation Studies of RNA Base and Ribose Nuclei Reveal a Complex Pattern of Motions in the RNA' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 15890361 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Shajani Zahra . . 2 Varani Gabriele . . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 349 _Journal_issue 4 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 699 _Page_last 715 _Year 2005 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'U1A Protein' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label '3'-untranslated region' $3'-UTR stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_3'-UTR _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common 3'-UTR _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 30 _Mol_residue_sequence ; GGCAGAGUCCUUCGGGACAU UGCACCUGCC ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 19 G 2 20 G 3 21 C 4 22 A 5 23 G 6 24 A 7 25 G 8 26 U 9 27 C 10 28 C 11 29 U 12 30 U 13 33 C 14 34 G 15 35 G 16 36 G 17 37 A 18 38 C 19 39 A 20 40 U 21 41 U 22 42 G 23 43 C 24 44 A 25 45 C 26 46 C 27 47 U 28 48 G 29 49 C 30 50 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $3'-UTR human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $3'-UTR 'enzymatic semisynthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $3'-UTR 0.7 mM '[U-13C; U-15N]' 'potassium phosphate' 10 mM 'natural abundance' EDTA .01 mM 'natural abundance' H2O 99.9 v/v 'natural abundance' D20 .1 v/v 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $3'-UTR 1.0 mM '[U-13C; U-15N]' 'potassium phosphate' 10 mM 'natural abundance' EDTA .01 mM 'natural abundance' H2O 99.9 v/v 'natural abundance' D20 .1 v/v 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_ModelFree _Saveframe_category software _Name ModelFree _Version . loop_ _Vendor _Address _Electronic_address Palmer . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 500 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 750 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-13C_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_13C-{1H}_NOE_5 _Saveframe_category NMR_applied_experiment _Experiment_name '13C-{1H} NOE' _Sample_label $sample_1 save_ save_13C-{1H}_NOE_6 _Saveframe_category NMR_applied_experiment _Experiment_name '13C-{1H} NOE' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 298 . K pH 6.0 . pH pressure 1.0 . atm stop_ save_ save_heteronuclear_T1_list_1 _Saveframe_category T1_relaxation _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 500 _T1_coherence_type Nz _T1_value_units ms _Mol_system_component_name '3'-untranslated region' _Text_data_format . _Text_data . loop_ _T1_ID _Residue_seq_code _Residue_label _Atom_name _T1_value _T1_value_error 1 1 G C1' 368.3 25 2 1 G C5 397.5 12.6 3 2 G C5 391.1 27.1 4 3 C C1' 568.7 20.3 5 3 C C5 470.5 59.7 6 4 A C5 398.2 11.2 7 5 G C1' 49.5 11.8 8 6 A C1' 444.1 21.8 9 6 A C5 389.8 10.4 10 7 G C1' 577.2 14 11 7 G C5 410.1 11.6 12 8 U C1' 549.5 13.2 13 8 U C5 360.9 34.1 14 8 U C6 398.5 23.1 15 9 C C1' 530.7 17.7 16 9 C C5 441.1 18.1 17 9 C C6 359.7 10.5 18 10 C C1' 547.1 20.2 19 11 U C1' 495 18.3 20 11 U C5 361.9 17.3 21 11 U C6 354 16.2 22 12 U C1' 516.3 15.2 23 12 U C5 440.4 27.3 24 12 U C6 442.9 17.6 25 13 C C1' 562.6 25 26 13 C C5 434.2 27.5 27 13 C C6 387 7.9 28 14 G C1' 556.6 25.2 29 14 G C5 677.5 29.3 30 15 G C1' 580.3 60 31 15 G C5 424.3 9.91 32 17 A C1' 537.2 8.08 33 17 A C5 447.2 19.9 34 18 C C1' 461.4 27 35 18 C C5 490.8 64.5 36 18 C C6 415.7 23.4 37 19 A C5 443.5 8.71 38 20 U C1' 389.8 10.8 39 20 U C5 372.4 15.2 40 20 U C6 344.2 7.43 41 21 U C1' 349.2 8.61 42 21 U C5 309.5 9.89 43 21 U C6 277.9 6.97 44 22 G C1' 389.4 6.57 45 22 G C5 373.2 16.8 46 23 C C1' 451.4 40.7 47 23 C C5 310.3 16.6 48 23 C C6 309.5 16.3 49 24 A C1' 355 18 50 24 A C5 376.4 22.5 51 25 C C5 372.5 26.1 52 26 C C5 354 19.8 53 27 U C1' 498.1 20.6 54 27 U C5 407.1 20 55 28 G C5 352.8 12.5 56 29 C C1' 475.8 14.5 57 29 C C5 283 14.9 stop_ save_ save_heteronuclear_T1_list_2 _Saveframe_category T1_relaxation _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 750 _T1_coherence_type Nz _T1_value_units ms _Mol_system_component_name '3'-untranslated region' _Text_data_format . _Text_data . loop_ _T1_ID _Residue_seq_code _Residue_label _Atom_name _T1_value _T1_value_error 1 1 G C1' 877.2 212 2 1 G C8 574.2 18.8 3 2 G C8 662 37.1 4 3 C C1' 1122 64.9 5 4 A C8 752.4 19.2 6 5 G C1' 918.4 40.6 7 6 A C1' 757 30.9 8 6 A C8 629.7 26.4 9 7 G C1' 1234 60 10 7 G C8 686.2 34.3 11 8 U C1' 1095 76.5 12 8 U C6 535.9 58.4 13 9 C C1' 1094 69.5 14 9 C C6 566.2 37.2 15 10 C C1' 1134 56.7 16 11 U C1' 940.3 47 17 11 U C6 505.2 45.5 18 12 U C1' 1052 68.6 19 12 U C6 587 37.3 20 13 C C1' 1156 59.7 21 13 C C6 507 23.2 22 14 G C1' 1087 123 23 14 G C8 868 41.7 24 15 G C8 708.3 18.5 25 17 A C1' 1113 66.4 26 17 A C8 760.2 18.4 27 18 C C1' 1057 70.2 28 18 C C6 531.5 70 29 19 A C8 795.5 22.4 30 20 U C1' 700.1 18 31 20 U C6 422.3 29.2 32 21 U C1' 618.9 20 33 21 U C6 354 9.7 34 22 G C1' 702.3 25.6 35 22 G C8 528.1 32.4 36 23 C C6 416 37.7 37 24 A C8 655.6 19.8 38 26 C C1' 1134 81.9 39 27 U C1' 1029 68.8 40 28 G C8 590.2 24.4 41 29 C C1' 910.4 41.6 stop_ save_ save_heteronuclear_T1rho_list_1 _Saveframe_category T1rho_relaxation _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 500 _T1rho_coherence_type Nz _T1rho_value_units ms _Text_data_format . _Text_data . loop_ _T1rho_ID _Residue_seq_code _Residue_label _Atom_name _T1rho_value _T1rho_value_error 1 1 G C1' 57.6 4.4 2 1 G C5 31.1 0.8 3 2 G C5 30.3 0.6 4 3 C C1' 36.2 0.7 5 3 C C5 28.2 1.6 6 4 A C5 29.6 0.5 7 5 G C1' 35.7 0.3 8 6 A C1' 24 0.7 9 6 A C5 25.6 0.7 10 7 G C1' 38.5 0.3 11 7 G C5 26.8 0.7 12 8 U C1' 36.1 0.6 13 8 U C5 26.8 0.8 14 8 U C6 20.1 1.1 15 9 C C1' 36.1 0.5 16 9 C C5 30.1 1.7 17 9 C C6 21.8 0.7 18 10 C C1' 34.6 0.6 19 11 U C1' 36.1 0.9 20 11 U C5 26.9 1.1 21 11 U C6 19.2 0.7 22 12 U C1' 40.5 1.8 23 12 U C5 39.7 1.2 24 12 U C6 28.8 0.9 25 13 C C1' 31 0.9 26 13 C C5 31.4 3.1 27 13 C C6 24.7 1.2 28 14 G C1' 36.1 0.8 29 14 G C5 26.8 0.7 30 15 G C1' 34.7 1.4 31 15 G C5 33 0.5 32 17 A C1' 39.1 0.8 33 17 A C5 31.9 0.9 34 18 C C1' 37.7 1.1 35 18 C C5 60.6 1.9 36 18 C C6 20.6 1.3 37 19 A C5 29.4 0.4 38 20 U C1' 48.8 0.3 39 20 U C5 39.6 2.7 40 20 U C6 30.1 1 41 21 U C1' 56.3 0.8 42 21 U C5 54.3 2.6 43 21 U C6 46.4 1.6 44 22 G C1' 37 0.3 45 22 G C5 27 0.9 46 23 C C1' 20.5 2.3 47 23 C C5 44.6 5.9 48 23 C C6 25.3 1.3 49 24 A C1' 33.9 2 50 24 A C5 26.5 0.6 51 25 C C5 28.4 2.5 52 26 C C5 28.5 1.5 53 27 U C1' 30.9 0.4 54 27 U C5 32.8 1.8 55 28 G C5 31.2 0.7 56 29 C C1' 36.3 0.6 57 29 C C5 27.7 2.4 stop_ save_ save_heteronuclear_T1rho_list_2 _Saveframe_category T1rho_relaxation _Details . loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 750 _T1rho_coherence_type Nz _T1rho_value_units ms _Text_data_format . _Text_data . loop_ _T1rho_ID _Residue_seq_code _Residue_label _Atom_name _T1rho_value _T1rho_value_error 1 1 G C1' 50.8 7.9 2 1 G C8 19.5 0.8 3 2 G C8 16 1 4 3 C C1' 32 2 5 4 A C8 19.4 1 6 5 G C1' 27.1 3 7 6 A C1' 15.45 1.7 8 6 A C8 12.5 0.2 9 7 G C1' 28.6 2.1 10 7 G C8 19.5 0.4 11 8 U C1' 31.5 0.1 12 8 U C6 13.2 58.4 13 9 C C1' 32 1.2 14 9 C C6 15.2 37.2 15 10 C C1' 32.3 0.6 16 11 U C1' 39.5 0.8 17 11 U C6 18.7 45.5 18 12 U C1' 33.9 0.9 19 12 U C6 23.7 37.3 20 13 C C1' 34 1.7 21 13 C C6 20.5 23.2 22 14 G C1' 36.67 3.9 23 14 G C8 22.2 1.4 24 15 G C8 22 0.9 25 17 A C1' 36.4 0.8 26 17 A C8 19.5 0.6 27 18 C C1' 35.7 1.2 28 18 C C6 15.6 70 29 19 A C8 20.3 0.7 30 20 U C1' 45.3 1.1 31 20 U C6 22.9 29.2 32 21 U C1' 50.5 0.8 33 21 U C6 38.2 9.7 34 22 G C1' 27.4 0.3 35 22 G C8 24.9 1.1 36 23 C C6 15.1 37.7 37 24 A C8 13.1 0.6 38 26 C C1' 32.3 1 39 27 U C1' 32.6 3.4 40 28 G C8 15.6 0.99 41 29 C C1' 33.9 1.6 stop_ save_ save_heteronuclear_noe_list_1 _Saveframe_category heteronuclear_NOE _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '13C-{1H} NOE' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 500 _Mol_system_component_name '3'-untranslated region' _Atom_one_atom_name C5 _Atom_two_atom_name H _Heteronuclear_NOE_value_type 'peak height' _NOE_reference_value 1 _NOE_reference_description . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Heteronuclear_NOE_value _Heteronuclear_NOE_value_error 1 G 1.25 0.02 1 G 1.69 0.19 2 G 1.19 0.03 3 C 1.19 0.04 3 C 1.21 0.01 4 A 1.21 0.02 5 G 1.23 0.02 6 A 1.28 0.02 6 A 1.26 0.03 7 G 1.18 0.02 7 G 1.19 0.02 8 U 1.17 0.05 8 U 1.18 0.01 8 U 1.08 0.03 9 C 1.4 0.05 9 C 1.21 0.01 9 C 1.18 0.03 10 C 1.23 0.01 11 U 1.28 0.03 11 U 1.22 0.01 11 U 1.2 0.02 12 U 1.27 0.02 12 U 1.27 0.01 12 U 1.27 0.02 13 C 1.13 0.04 13 C 1.22 0.02 13 C 1.15 0.03 14 G 1.22 0.03 14 G 1.21 0.02 15 G 1.26 0.02 15 G 1.19 0.03 17 A 1.15 0.02 17 A 1.2 0.01 18 C 1.15 0.03 18 C 1.17 0.01 18 C 1.16 0.03 19 A 1.2 0.02 20 U 1.26 0.02 20 U 1.36 0.01 20 U 1.24 0.02 21 U 1.43 0.02 21 U 1.44 0.01 21 U 1.36 0.02 22 G 1.28 0.02 22 G 1.38 0.02 23 C 1.22 0.03 23 C 1.45 0.07 24 A 1.31 0.03 24 A 1.34 0.05 25 C 1.24 0.04 26 C 1.1 0.05 27 U 1.08 0.02 27 U 1.27 0.02 28 G 1.25 0.02 29 C 1.13 0.04 29 C 1.22 0.01 stop_ save_ save_heteronuclear_noe_list_2 _Saveframe_category heteronuclear_NOE _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '13C-{1H} NOE' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Spectrometer_frequency_1H 750 _Mol_system_component_name '3'-untranslated region' _Atom_one_atom_name C8 _Atom_two_atom_name H _Heteronuclear_NOE_value_type 'peak height' _NOE_reference_value 1 _NOE_reference_description . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Heteronuclear_NOE_value _Heteronuclear_NOE_value_error 1 G 1.16 0.01 1 G 2.26 0.2 2 G 1.24 0.01 3 C 1.36 0.02 4 A 1.09 0.01 5 G 1.44 0.03 6 A 1.11 0.01 6 A 1.34 0.06 7 G 1.06 0.01 7 G 1.38 0.05 8 U 1.35 0.01 8 U 1.15 0.01 9 C 1.36 0.01 9 C 1.12 0.01 10 C 1.44 0.02 11 U 1.37 0.02 11 U 1.12 0.01 12 U 1.4 0.02 12 U 1.21 0.01 13 C 1.37 0.03 14 G 1 0.01 14 G 1.31 0.02 15 G 1.14 0.01 17 A 1.1 0.01 17 A 1.27 0.02 18 C 1.37 0.02 18 C 1.07 0.01 19 A 1.13 0.01 20 U 1.55 0.02 20 U 1.11 0.01 21 U 1.62 0.02 21 U 1.27 0.01 22 G 1.31 0.01 22 G 1.44 0.03 23 C 1.43 0.11 24 A 1.17 0.01 24 A 1.56 0.07 26 C 1.34 0.09 27 U 1.36 0.03 28 G 1.18 0.01 29 C 1.43 0.02 stop_ save_ save_order_parameter_list_1 _Saveframe_category S2_parameters _Details . _Sample_conditions_label $sample_conditions_1 _Mol_system_component_name '3'-untranslated region' _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 1 G C1' . 0.58 0.05 . . . . . . . . . . . . 1 G C5 . 0.88 0.02 . . . . . . . . . . . . 2 G C5 . 0.66 0.05 . . . . . . . . . . . . 3 C C1' . 0.99 0.02 . . . . . . . . . . . . 4 A C5 . 0.89 0.02 . . . . . . . . . . . . 6 A C1' . 0.88 0.03 . . . . . . . . . . . . 6 A C5 . 0.86 0.02 . . . . . . . . . . . . 7 G C1' . 0.90 0.02 . . . . . . . . . . . . 8 U C1' . 1.00 0.01 . . . . . . . . . . . . 9 C C1' . 0.97 0.03 . . . . . . . . . . . . 9 C C5 . 0.93 0.03 . . . . . . . . . . . . 9 C C6 . 0.88 0.02 . . . . . . . . . . . . 10 C C1' . 0.96 0.01 . . . . . . . . . . . . 11 U C1' . 0.95 0.02 . . . . . . . . . . . . 11 U C5 . 0.81 0.04 . . . . . . . . . . . . 11 U C6 . 0.86 0.03 . . . . . . . . . . . . 12 U C1' . 0.89 0.03 . . . . . . . . . . . . 12 U C5 . 0.78 0.03 . . . . . . . . . . . . 12 U C6 . 0.89 0.03 . . . . . . . . . . . . 13 C C1' . 0.91 0.04 . . . . . . . . . . . . 13 C C5 . 1.00 0.06 . . . . . . . . . . . . 14 G C1' . 0.93 0.04 . . . . . . . . . . . . 14 G C5 . 0.66 0.03 . . . . . . . . . . . . 15 G C1' . 0.90 0.09 . . . . . . . . . . . . 15 G C5 . 0.92 0.01 . . . . . . . . . . . . 17 A C1' . 0.95 0.01 . . . . . . . . . . . . 17 A C5 . 1.00 0.02 . . . . . . . . . . . . 18 C C1' . 0.93 0.04 . . . . . . . . . . . . 18 C C5 . 0.99 0.03 . . . . . . . . . . . . 18 C C6 . 0.97 0.03 . . . . . . . . . . . . 19 A C5 . 0.96 0.01 . . . . . . . . . . . . 20 U C1' . 0.69 0.02 . . . . . . . . . . . . 20 U C5 . 0.83 0.03 . . . . . . . . . . . . 20 U C6 . 0.94 0.01 . . . . . . . . . . . . 21 U C1' . 0.57 0.02 . . . . . . . . . . . . 21 U C5 . 0.54 0.02 . . . . . . . . . . . . 21 U C6 . 0.54 0.02 . . . . . . . . . . . . 22 G C1' . 0.76 0.01 . . . . . . . . . . . . 22 G C5 . 0.83 0.03 . . . . . . . . . . . . 23 C C1' . 0.84 0.05 . . . . . . . . . . . . 23 C C5 . 0.62 0.09 . . . . . . . . . . . . 23 C C6 . 0.74 0.04 . . . . . . . . . . . . 24 A C1' . 0.70 0.04 . . . . . . . . . . . . 24 A C5 . 0.82 0.04 . . . . . . . . . . . . 25 C C5 . 0.84 0.05 . . . . . . . . . . . . 27 U C1' . 0.94 0.02 . . . . . . . . . . . . 27 U C5 . 1.00 0.08 . . . . . . . . . . . . 28 G C5 . 0.80 0.03 . . . . . . . . . . . . 29 C C1' . 0.93 0.02 . . . . . . . . . . . . stop_ save_ save_order_parameter_list_2 _Saveframe_category S2_parameters _Details . _Sample_conditions_label $sample_conditions_1 _Mol_system_component_name '3'-untranslated region' _Tau_e_value_units . _Tau_s_value_units . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _Model_fit _S2_value _S2_value_fit_error _Tau_e_value _Tau_e_value_fit_error _S2f_value _S2f_value_fit_error _S2s_value _S2s_value_fit_error _Tau_s_value _Tau_s_value_fit_error _S2H_value _S2H_value_fit_error _S2N_value _S2N_value_fit_error 1 G C1' . 0.56 0.08 . . . . . . . . . . . . 3 C C1' . 0.94 0.01 . . . . . . . . . . . . 5 G C1' . 0.86 0.02 . . . . . . . . . . . . 6 A C1' . 0.79 0.02 . . . . . . . . . . . . 7 G C1' . 0.90 0.04 . . . . . . . . . . . . 9 C C1' . 0.93 0.02 . . . . . . . . . . . . 12 U C1' . 0.91 0.02 . . . . . . . . . . . . 13 C C1' . 0.94 0.01 . . . . . . . . . . . . 14 G C1' . 0.93 0.03 . . . . . . . . . . . . 17 A C1' . 0.88 0.02 . . . . . . . . . . . . 18 C C1' . 0.91 0.02 . . . . . . . . . . . . 20 U C1' . 0.68 0.02 . . . . . . . . . . . . 21 U C1' . 0.60 0.02 . . . . . . . . . . . . 22 G C1' . 0.74 0.02 . . . . . . . . . . . . 27 U C1' . 0.91 0.02 . . . . . . . . . . . . 29 C C1' . 0.86 0.02 . . . . . . . . . . . . stop_ save_