data_26000 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution structure of the delta-J-delta-K domain of EMCV IRES ; _BMRB_accession_number 26000 _BMRB_flat_file_name bmr26000.str _Entry_type original _Submission_date 2016-03-16 _Accession_date 2016-03-23 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Imai Shunsuke . . 2 D'Souza Victoria . . 3 Wagner Gerhard . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 274 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2016-10-12 update BMRB 'update entry citation' 2016-08-08 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 25996 'Solution structure of the J-K region of EMCV IRES' 25998 'Solution structure of the K domain of EMCV IRES' 25999 'Solution structure of the St domain of EMCV IRES' 26997 'Solution structure of the J domain of EMCV IRES' stop_ _Original_release_date 2016-08-08 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; An accurately preorganized IRES RNA structure enables eIF4G capture for initiation of viral translation ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 27525590 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Imai Shunsuke . . 2 Hellen Christopher . . 3 D'Souza Victoria . . 4 Wagner Gerhard . . stop_ _Journal_abbreviation 'Nat. Struct. Mol. Biol.' _Journal_volume 23 _Journal_issue 9 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 859 _Page_last 864 _Year 2016 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'delta-J-delta-K domain of EMCV IRES' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'RNA (67-MER)' $RNA_(67-MER) stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_RNA_(67-MER) _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common RNA_(67-MER) _Molecular_mass 21821.207 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 67 _Mol_residue_sequence ; GGGGCUGAAGGAUGCCCAGA GAGAUCUGGGGCCUCGGGAG AUCGAGGUUAAAAAACGUCU AGGCCCC ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 680 G 2 681 G 3 682 G 4 683 G 5 684 C 6 685 U 7 686 G 8 687 A 9 688 A 10 689 G 11 690 G 12 691 A 13 692 U 14 693 G 15 694 C 16 695 C 17 696 C 18 697 A 19 698 G 20 699 A 21 700 G 22 701 A 23 702 G 24 703 A 25 725 U 26 726 C 27 727 U 28 728 G 29 729 G 30 730 G 31 731 G 32 732 C 33 733 C 34 734 U 35 735 C 36 736 G 37 737 G 38 738 G 39 739 A 40 740 G 41 741 A 42 762 U 43 763 C 44 764 G 45 765 A 46 766 G 47 767 G 48 768 U 49 769 U 50 770 A 51 771 A 52 772 A 53 773 A 54 774 A 55 775 A 56 776 C 57 777 G 58 778 U 59 779 C 60 780 U 61 781 A 62 782 G 63 783 G 64 784 C 65 785 C 66 786 C 67 787 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $RNA_(67-MER) 'Encephalomyocarditis virus' 12104 Viruses . Cardiovirus . stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $RNA_(67-MER) 'enzymatic semisynthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(67-MER) 0.5 mM 'natural abundance' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' D2O 100 % [U-2H] stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(67-MER) 0.5 mM 'natural abundance' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % [U-2H] stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(67-MER) 0.5 mM "[U-2H, {H1',H2',H2,H8}-Ade]" 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' D2O 100 % [U-2H] stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(67-MER) 0.5 mM '[U-2H]-Cyt, [U-2H]-Ura' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' D2O 100 % [U-2H] stop_ save_ save_sample_5 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(67-MER) 0.5 mM '[U-2H]-Gua, [U-2H]-Ura' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' D2O 100 % [U-2H] stop_ save_ save_sample_6 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(67-MER) 0.5 mM "[U-2H, {H1',H2',H2,H8}-Ade]" 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % [U-2H] stop_ save_ ############################ # Computer software used # ############################ save_CARA _Saveframe_category software _Name CARA _Version . loop_ _Vendor _Address _Electronic_address 'Keller and Wuthrich' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_CYANA _Saveframe_category software _Name CYANA _Version . loop_ _Vendor _Address _Electronic_address 'Guntert, Mumenthaler and Wuthrich' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ save_X-PLOR_NIH _Saveframe_category software _Name X-PLOR_NIH _Version . loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task refinement stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 700 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_3 save_ save_2D_1H-1H_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_4 save_ save_2D_1H-1H_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_5 save_ save_2D_1H-1H_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_2D_1H-1H_NOESY_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_2D_1H-1H_NOESY_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_6 save_ save_2D_1H-1H_NOESY_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_6 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 6.5 . pH pressure 1 . atm temperature 308 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 6.5 . pH pressure 1 . atm temperature 283 . K stop_ save_ save_sample_conditions_3 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 5.5 . pH pressure 1 . atm temperature 283 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0 external direct . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'RNA (67-MER)' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 680 1 G H1' H 5.79 0.03 1 2 680 1 G H2' H 4.86 0.03 1 3 680 1 G H8 H 8.08 0.03 1 4 681 2 G H1' H 5.89 0.03 1 5 681 2 G H8 H 7.52 0.03 1 6 682 3 G H1' H 5.76 0.03 1 7 682 3 G H8 H 7.18 0.03 1 8 683 4 G H1' H 5.72 0.03 1 9 683 4 G H8 H 7.15 0.03 1 10 684 5 C H1' H 5.49 0.03 1 11 684 5 C H5 H 5.10 0.03 1 12 684 5 C H6 H 7.44 0.03 1 13 685 6 U H1' H 5.52 0.03 1 14 685 6 U H2' H 4.29 0.03 1 15 685 6 U H5 H 5.53 0.03 1 16 685 6 U H6 H 7.57 0.03 1 17 686 7 G H1' H 5.31 0.03 1 18 686 7 G H8 H 7.67 0.03 1 19 687 8 A H1' H 5.70 0.03 1 20 687 8 A H2 H 7.50 0.03 1 21 687 8 A H2' H 4.46 0.03 1 22 687 8 A H8 H 7.94 0.03 1 23 688 9 A H1' H 5.89 0.03 1 24 688 9 A H2 H 8.06 0.03 1 25 688 9 A H2' H 4.80 0.03 1 26 688 9 A H8 H 8.26 0.03 1 27 689 10 G H1' H 5.52 0.03 1 28 689 10 G H8 H 7.75 0.03 1 29 690 11 G H1' H 5.58 0.03 1 30 690 11 G H8 H 7.26 0.03 1 31 691 12 A H1' H 5.95 0.03 1 32 691 12 A H2 H 7.80 0.03 1 33 691 12 A H8 H 7.65 0.03 1 34 692 13 U H1' H 5.25 0.03 1 35 692 13 U H2' H 4.07 0.03 1 36 692 13 U H5 H 5.34 0.03 1 37 692 13 U H6 H 7.45 0.03 1 38 693 14 G H1' H 5.84 0.03 1 39 693 14 G H2' H 4.74 0.03 1 40 693 14 G H3' H 4.50 0.03 1 41 693 14 G H8 H 7.88 0.03 1 42 694 15 C H1' H 5.50 0.03 1 43 694 15 C H5 H 5.84 0.03 1 44 694 15 C H6 H 7.87 0.03 1 45 695 16 C H1' H 5.49 0.03 1 46 695 16 C H5 H 5.58 0.03 1 47 695 16 C H6 H 7.87 0.03 1 48 696 17 C H1' H 5.41 0.03 1 49 696 17 C H2' H 4.53 0.03 1 50 696 17 C H5 H 5.53 0.03 1 51 696 17 C H6 H 7.73 0.03 1 52 697 18 A H1' H 5.85 0.03 1 53 697 18 A H2 H 6.85 0.03 1 54 697 18 A H2' H 4.45 0.03 1 55 697 18 A H3' H 4.59 0.03 1 56 697 18 A H4' H 4.51 0.03 1 57 697 18 A H5' H 4.10 0.03 2 58 697 18 A H5'' H 4.12 0.03 2 59 697 18 A H8 H 7.97 0.03 1 60 698 19 G H1' H 5.53 0.03 1 61 698 19 G H2' H 4.32 0.03 1 62 698 19 G H3' H 4.47 0.03 1 63 698 19 G H4' H 4.39 0.03 1 64 698 19 G H5' H 4.02 0.03 2 65 698 19 G H5'' H 4.04 0.03 2 66 698 19 G H8 H 7.11 0.03 1 67 699 20 A H1' H 5.87 0.03 1 68 699 20 A H2 H 7.69 0.03 1 69 699 20 A H2' H 4.48 0.03 1 70 699 20 A H3' H 4.39 0.03 1 71 699 20 A H4' H 4.47 0.03 1 72 699 20 A H5' H 4.00 0.03 2 73 699 20 A H5'' H 4.07 0.03 2 74 699 20 A H8 H 7.53 0.03 1 75 700 21 G H1' H 5.40 0.03 1 76 700 21 G H2' H 4.20 0.03 1 77 700 21 G H3' H 4.46 0.03 1 78 700 21 G H4' H 4.34 0.03 1 79 700 21 G H5' H 4.26 0.03 2 80 700 21 G H5'' H 4.27 0.03 2 81 700 21 G H8 H 7.04 0.03 1 82 701 22 A H1' H 5.68 0.03 1 83 701 22 A H2 H 7.89 0.03 1 84 701 22 A H2' H 4.58 0.03 1 85 701 22 A H3' H 4.34 0.03 1 86 701 22 A H4' H 4.15 0.03 1 87 701 22 A H5' H 4.16 0.03 2 88 701 22 A H5'' H 3.84 0.03 2 89 701 22 A H8 H 8.14 0.03 1 90 702 23 G H1' H 5.22 0.03 1 91 702 23 G H2' H 4.22 0.03 1 92 702 23 G H3' H 4.48 0.03 1 93 702 23 G H4' H 4.21 0.03 1 94 702 23 G H5' H 4.02 0.03 2 95 702 23 G H5'' H 3.84 0.03 2 96 702 23 G H8 H 7.55 0.03 1 97 703 24 A H1' H 6.05 0.03 1 98 703 24 A H2 H 8.16 0.03 1 99 703 24 A H2' H 4.44 0.03 1 100 703 24 A H3' H 4.85 0.03 1 101 703 24 A H4' H 4.39 0.03 1 102 703 24 A H5' H 4.22 0.03 2 103 703 24 A H5'' H 4.20 0.03 2 104 703 24 A H8 H 8.12 0.03 1 105 725 25 U H1' H 4.19 0.03 1 106 725 25 U H5 H 5.88 0.03 1 107 725 25 U H6 H 7.94 0.03 1 108 726 26 C H1' H 5.50 0.03 1 109 726 26 C H5 H 5.69 0.03 1 110 726 26 C H6 H 7.75 0.03 1 111 727 27 U H1' H 5.41 0.03 1 112 727 27 U H2' H 4.50 0.03 1 113 727 27 U H5 H 5.33 0.03 1 114 727 27 U H6 H 7.74 0.03 1 115 728 28 G H1' H 5.70 0.03 1 116 728 28 G H2' H 4.52 0.03 1 117 728 28 G H3' H 4.41 0.03 1 118 728 28 G H4' H 4.07 0.03 1 119 728 28 G H5' H 4.00 0.03 2 120 728 28 G H5'' H 4.06 0.03 2 121 728 28 G H8 H 7.60 0.03 1 122 729 29 G H1' H 5.62 0.03 1 123 729 29 G H2' H 4.52 0.03 1 124 729 29 G H8 H 7.06 0.03 1 125 730 30 G H1' H 5.73 0.03 1 126 730 30 G H5' H 4.00 0.03 2 127 730 30 G H8 H 7.05 0.03 1 128 731 31 G H1' H 5.68 0.03 1 129 731 31 G H2' H 4.43 0.03 1 130 731 31 G H3' H 4.55 0.03 1 131 731 31 G H8 H 7.38 0.03 1 132 732 32 C H1' H 5.39 0.03 1 133 732 32 C H2' H 4.26 0.03 1 134 732 32 C H3' H 4.44 0.03 1 135 732 32 C H4' H 4.34 0.03 1 136 732 32 C H5 H 5.41 0.03 1 137 732 32 C H5' H 4.04 0.03 2 138 732 32 C H5'' H 4.05 0.03 2 139 732 32 C H6 H 7.73 0.03 1 140 733 33 C H1' H 5.51 0.03 1 141 733 33 C H5 H 5.47 0.03 1 142 733 33 C H6 H 7.79 0.03 1 143 734 34 U H1' H 5.47 0.03 1 144 734 34 U H2' H 4.42 0.03 1 145 734 34 U H5 H 5.32 0.03 1 146 734 34 U H6 H 7.85 0.03 1 147 735 35 C H1' H 5.50 0.03 1 148 735 35 C H5 H 5.57 0.03 1 149 735 35 C H6 H 7.79 0.03 1 150 736 36 G H1' H 5.59 0.03 1 151 736 36 G H8 H 7.44 0.03 1 152 737 37 G H1' H 5.56 0.03 1 153 737 37 G H8 H 7.16 0.03 1 154 738 38 G H1' H 5.53 0.03 1 155 738 38 G H8 H 7.39 0.03 1 156 739 39 A H1' H 5.76 0.03 1 157 739 39 A H2 H 7.94 0.03 1 158 739 39 A H8 H 8.17 0.03 1 159 740 40 G H1' H 5.47 0.03 1 160 740 40 G H8 H 7.70 0.03 1 161 741 41 A H1' H 5.97 0.03 1 162 741 41 A H2 H 8.04 0.03 1 163 741 41 A H2' H 4.51 0.03 1 164 741 41 A H8 H 8.21 0.03 1 165 762 42 U H1' H 5.38 0.03 1 166 762 42 U H2' H 4.28 0.03 1 167 762 42 U H5 H 5.81 0.03 1 168 762 42 U H6 H 7.91 0.03 1 169 763 43 C H1' H 5.46 0.03 1 170 763 43 C H5 H 5.81 0.03 1 171 763 43 C H6 H 7.83 0.03 1 172 764 44 G H1' H 5.59 0.03 1 173 764 44 G H8 H 7.48 0.03 1 174 765 45 A H1' H 5.86 0.03 1 175 765 45 A H2 H 7.36 0.03 1 176 765 45 A H2' H 4.41 0.03 1 177 765 45 A H3' H 4.59 0.03 1 178 765 45 A H8 H 7.66 0.03 1 179 766 46 G H1' H 5.50 0.03 1 180 766 46 G H8 H 6.93 0.03 1 181 767 47 G H1' H 5.66 0.03 1 182 767 47 G H2' H 4.40 0.03 1 183 767 47 G H3' H 4.63 0.03 1 184 767 47 G H4' H 4.07 0.03 1 185 767 47 G H5'' H 3.96 0.03 2 186 767 47 G H8 H 7.02 0.03 1 187 768 48 U H1' H 5.66 0.03 1 188 768 48 U H5 H 5.26 0.03 1 189 768 48 U H6 H 7.52 0.03 1 190 769 49 U H1' H 5.74 0.03 1 191 769 49 U H2' H 4.27 0.03 1 192 769 49 U H3' H 4.50 0.03 1 193 769 49 U H4' H 4.32 0.03 1 194 769 49 U H5 H 5.63 0.03 1 195 769 49 U H6 H 7.67 0.03 1 196 770 50 A H1' H 5.76 0.03 1 197 770 50 A H2 H 7.72 0.03 1 198 770 50 A H2' H 4.40 0.03 1 199 770 50 A H3' H 4.64 0.03 1 200 770 50 A H4' H 4.23 0.03 1 201 770 50 A H5' H 4.08 0.03 2 202 770 50 A H8 H 8.15 0.03 1 203 771 51 A H1' H 5.66 0.03 1 204 771 51 A H2 H 7.91 0.03 1 205 771 51 A H2' H 4.48 0.03 1 206 771 51 A H3' H 4.31 0.03 1 207 771 51 A H4' H 4.07 0.03 1 208 771 51 A H5' H 4.26 0.03 2 209 771 51 A H5'' H 4.21 0.03 2 210 771 51 A H8 H 7.98 0.03 1 211 772 52 A H1' H 5.60 0.03 1 212 772 52 A H2 H 7.72 0.03 1 213 772 52 A H2' H 4.47 0.03 1 214 772 52 A H3' H 4.33 0.03 1 215 772 52 A H4' H 4.45 0.03 1 216 772 52 A H5' H 4.20 0.03 2 217 772 52 A H5'' H 4.05 0.03 2 218 772 52 A H8 H 7.92 0.03 1 219 773 53 A H1' H 5.65 0.03 1 220 773 53 A H2 H 7.71 0.03 1 221 773 53 A H2' H 4.53 0.03 1 222 773 53 A H3' H 4.40 0.03 1 223 773 53 A H4' H 4.25 0.03 1 224 773 53 A H5' H 4.09 0.03 2 225 773 53 A H5'' H 4.08 0.03 2 226 773 53 A H8 H 7.91 0.03 1 227 774 54 A H1' H 5.65 0.03 1 228 774 54 A H2 H 7.75 0.03 1 229 774 54 A H2' H 4.44 0.03 1 230 774 54 A H3' H 4.58 0.03 1 231 774 54 A H4' H 4.31 0.03 1 232 774 54 A H5' H 4.14 0.03 2 233 774 54 A H5'' H 4.13 0.03 2 234 774 54 A H8 H 7.89 0.03 1 235 775 55 A H1' H 5.61 0.03 1 236 775 55 A H2 H 7.90 0.03 1 237 775 55 A H2' H 4.53 0.03 1 238 775 55 A H8 H 7.88 0.03 1 239 776 56 C H1' H 5.36 0.03 1 240 776 56 C H2' H 4.41 0.03 1 241 776 56 C H3' H 4.36 0.03 1 242 776 56 C H5 H 5.43 0.03 1 243 776 56 C H6 H 7.54 0.03 1 244 777 57 G H1' H 5.73 0.03 1 245 777 57 G H8 H 7.62 0.03 1 246 778 58 U H1' H 5.53 0.03 1 247 778 58 U H2' H 4.33 0.03 1 248 778 58 U H5 H 5.20 0.03 1 249 778 58 U H6 H 7.68 0.03 1 250 779 59 C H1' H 5.56 0.03 1 251 779 59 C H5 H 5.54 0.03 1 252 779 59 C H6 H 7.74 0.03 1 253 780 60 U H1' H 5.62 0.03 1 254 780 60 U H2' H 4.31 0.03 1 255 780 60 U H5 H 5.45 0.03 1 256 780 60 U H6 H 7.67 0.03 1 257 781 61 A H1' H 5.82 0.03 1 258 781 61 A H2 H 7.41 0.03 1 259 781 61 A H8 H 8.10 0.03 1 260 782 62 G H1' H 5.62 0.03 1 261 782 62 G H8 H 7.79 0.03 1 262 783 63 G H1' H 5.73 0.03 1 263 783 63 G H8 H 7.33 0.03 1 264 784 64 C H1' H 5.53 0.03 1 265 784 64 C H2' H 4.40 0.03 1 266 784 64 C H5 H 5.22 0.03 1 267 784 64 C H6 H 7.64 0.03 1 268 785 65 C H5 H 5.51 0.03 1 269 785 65 C H6 H 7.77 0.03 1 270 786 66 C H5 H 5.48 0.03 1 271 786 66 C H6 H 7.80 0.03 1 272 787 67 C H1' H 5.72 0.03 1 273 787 67 C H5 H 5.47 0.03 1 274 787 67 C H6 H 7.66 0.03 1 stop_ save_