data_25998 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution structure of the K domain of EMCV IRES ; _BMRB_accession_number 25998 _BMRB_flat_file_name bmr25998.str _Entry_type original _Submission_date 2016-03-16 _Accession_date 2016-03-23 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Imai Shunsuke . . 2 D'Souza Victoria . . 3 Wagner Gerhard . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 176 "13C chemical shifts" 167 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2016-10-12 update BMRB 'update entry citation' 2016-08-08 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 25996 'Solution structure of the J-K region of EMCV IRES' 25999 'Solution structure of the St domain of EMCV IRES' 26000 'Solution structure of the delta-J-delta-K domain of EMCV IRES' 26997 'Solution structure of the J domain of EMCV IRES' stop_ _Original_release_date 2016-08-08 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; An accurately preorganized IRES RNA structure enables eIF4G capture for initiation of viral translation ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 27525590 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Imai Shunsuke . . 2 Hellen Christopher . . 3 D'Souza Victoria . . 4 Wagner Gerhard . . stop_ _Journal_abbreviation 'Nat. Struct. Mol. Biol.' _Journal_volume 23 _Journal_issue 9 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 859 _Page_last 864 _Year 2016 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'K domain of EMCV IRES' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'RNA (40-MER)' $RNA_(40-MER) stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_RNA_(40-MER) _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common RNA_(40-MER) _Molecular_mass 12798.640 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 40 _Mol_residue_sequence ; GGGCUCGGUGCACAUGCUUU ACAUGUGUUUAGUCGAGCCC ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 730 G 2 731 G 3 732 G 4 733 C 5 734 U 6 735 C 7 736 G 8 737 G 9 738 U 10 739 G 11 740 C 12 741 A 13 742 C 14 743 A 15 744 U 16 745 G 17 746 C 18 747 U 19 748 U 20 749 U 21 750 A 22 751 C 23 752 A 24 753 U 25 754 G 26 755 U 27 756 G 28 757 U 29 758 U 30 759 U 31 760 A 32 761 G 33 762 U 34 763 C 35 764 G 36 765 A 37 766 G 38 767 C 39 768 C 40 769 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $RNA_(40-MER) 'Encephalomyocarditis virus' 12104 Viruses . Cardiovirus . stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $RNA_(40-MER) 'enzymatic semisynthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(40-MER) 0.5 mM 'natural abundance' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' D2O 100 % [U-2H] stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(40-MER) 0.5 mM 'natural abundance' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % [U-2H] stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(40-MER) 0.5 mM '[U-13C; U-15N]-Ade, [U-13C; U-15N]-Cyt' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' D2O 100 % [U-2H] stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(40-MER) 0.5 mM '[U-13C; U-15N]-Gua, [U-13C; U-15N]-Ura' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' D2O 100 % [U-2H] stop_ save_ save_sample_5 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(40-MER) 0.5 mM '[U-13C; U-15N]-Ade, [U-13C; U-15N]-Cyt' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % [U-2H] stop_ save_ save_sample_6 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $RNA_(40-MER) 0.5 mM '[U-13C; U-15N]-Gua, [U-13C; U-15N]-Ura' 'potassium phosphate' 10 mM 'natural abundance' 'sodium chloride' 10 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % [U-2H] stop_ save_ ############################ # Computer software used # ############################ save_CARA _Saveframe_category software _Name CARA _Version . loop_ _Vendor _Address _Electronic_address 'Keller and Wuthrich' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_CYANA _Saveframe_category software _Name CYANA _Version . loop_ _Vendor _Address _Electronic_address 'Guntert, Mumenthaler and Wuthrich' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ save_X-PLOR_NIH _Saveframe_category software _Name X-PLOR_NIH _Version . loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task refinement stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 700 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_2D_1H-1H_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_2D_1H-13C_HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_3 save_ save_3D_1H-13C_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_3 save_ save_2D_1H-13C_HSQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_4 save_ save_3D_1H-13C_NOESY_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_4 save_ save_2D_1H-15N_HSQC_8 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_5 save_ save_2D_1H-15N_HSQC_9 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_5 save_ save_2D_1H-15N_HSQC_10 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_6 save_ save_2D_1H-15N_HSQC_11 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_6 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 6.5 . pH pressure 1 . atm temperature 308 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 6.5 . pH pressure 1 . atm temperature 283 . K stop_ save_ save_sample_conditions_3 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 5.5 . pH pressure 1 . atm temperature 283 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0 external direct . . . 1 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '2D 1H-13C HSQC' stop_ loop_ _Sample_label $sample_1 $sample_3 $sample_4 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'RNA (40-MER)' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 730 1 G H1' H 5.83 0.03 1 2 730 1 G H2' H 4.92 0.03 1 3 730 1 G H8 H 8.14 0.03 1 4 730 1 G C1' C 88.64 0.1 1 5 730 1 G C2' C 72.43 0.1 1 6 730 1 G C8 C 136.43 0.1 1 7 731 2 G H1' H 5.94 0.03 1 8 731 2 G H2' H 4.71 0.03 1 9 731 2 G H8 H 7.59 0.03 1 10 731 2 G C1' C 90.07 0.1 1 11 731 2 G C2' C 72.95 0.1 1 12 731 2 G C8 C 134.23 0.1 1 13 732 3 G H1' H 5.80 0.03 1 14 732 3 G H2' H 4.54 0.03 1 15 732 3 G H8 H 7.29 0.03 1 16 732 3 G C1' C 90.34 0.1 1 17 732 3 G C2' C 72.66 0.1 1 18 732 3 G C8 C 133.33 0.1 1 19 733 4 C H1' H 5.53 0.03 1 20 733 4 C H2' H 4.46 0.03 1 21 733 4 C H5 H 5.26 0.03 1 22 733 4 C H6 H 7.59 0.03 1 23 733 4 C C1' C 91.43 0.1 1 24 733 4 C C2' C 72.74 0.1 1 25 733 4 C C5 C 94.57 0.1 1 26 733 4 C C6 C 138.28 0.1 1 27 734 5 U H1' H 5.57 0.03 1 28 734 5 U H2' H 4.53 0.03 1 29 734 5 U H5 H 5.44 0.03 1 30 734 5 U H6 H 7.87 0.03 1 31 734 5 U C1' C 91.24 0.1 1 32 734 5 U C2' C 72.63 0.1 1 33 734 5 U C5 C 100.77 0.1 1 34 734 5 U C6 C 139.29 0.1 1 35 735 6 C H1' H 5.54 0.03 1 36 735 6 C H2' H 4.51 0.03 1 37 735 6 C H5 H 5.64 0.03 1 38 735 6 C H6 H 7.82 0.03 1 39 735 6 C C1' C 91.07 0.1 1 40 735 6 C C2' C 70.05 0.1 1 41 735 6 C C5 C 95.23 0.1 1 42 735 6 C C6 C 138.59 0.1 1 43 736 7 G H1' H 5.65 0.03 1 44 736 7 G H2' H 4.51 0.03 1 45 736 7 G H8 H 7.52 0.03 1 46 736 7 G C1' C 90.14 0.1 1 47 736 7 G C2' C 72.78 0.1 1 48 736 7 G C8 C 133.70 0.1 1 49 737 8 G H1' H 5.75 0.03 1 50 737 8 G H2' H 4.46 0.03 1 51 737 8 G H3' H 4.42 0.03 1 52 737 8 G H4' H 4.45 0.03 1 53 737 8 G H8 H 7.22 0.03 1 54 737 8 G C1' C 90.16 0.1 1 55 737 8 G C2' C 72.86 0.1 1 56 737 8 G C3' C 71.03 0.1 1 57 737 8 G C4' C 80.31 0.1 1 58 737 8 G C8 C 134.75 0.1 1 59 738 9 U H1' H 5.65 0.03 1 60 738 9 U H2' H 4.47 0.03 1 61 738 9 U H3' H 4.59 0.03 1 62 738 9 U H5 H 5.45 0.03 1 63 738 9 U H5' H 4.09 0.03 2 64 738 9 U H5'' H 4.46 0.03 2 65 738 9 U H6 H 7.69 0.03 1 66 738 9 U C1' C 90.06 0.1 1 67 738 9 U C2' C 73.11 0.1 1 68 738 9 U C3' C 70.85 0.1 1 69 738 9 U C5 C 101.61 0.1 1 70 738 9 U C5' C 62.98 0.1 1 71 738 9 U C6 C 138.57 0.1 1 72 739 10 G H1' H 5.69 0.03 1 73 739 10 G H2' H 4.67 0.03 1 74 739 10 G H3' H 4.41 0.03 1 75 739 10 G H4' H 4.50 0.03 1 76 739 10 G H8 H 7.82 0.03 1 77 739 10 G C1' C 90.48 0.1 1 78 739 10 G C2' C 72.36 0.1 1 79 739 10 G C3' C 71.02 0.1 1 80 739 10 G C4' C 80.10 0.1 1 81 739 10 G C8 C 134.93 0.1 1 82 740 11 C H1' H 5.40 0.03 1 83 740 11 C H2' H 4.39 0.03 1 84 740 11 C H5 H 5.45 0.03 1 85 740 11 C H6 H 7.71 0.03 1 86 740 11 C C1' C 91.06 0.1 1 87 740 11 C C2' C 72.64 0.1 1 88 740 11 C C5 C 95.05 0.1 1 89 740 11 C C6 C 138.02 0.1 1 90 741 12 A H1' H 5.95 0.03 1 91 741 12 A H2 H 7.37 0.03 1 92 741 12 A H2' H 4.55 0.03 1 93 741 12 A H8 H 8.05 0.03 1 94 741 12 A C1' C 90.24 0.1 1 95 741 12 A C2 C 150.63 0.1 1 96 741 12 A C2' C 72.93 0.1 1 97 741 12 A C8 C 136.74 0.1 1 98 742 13 C H1' H 5.37 0.03 1 99 742 13 C H2' H 4.39 0.03 1 100 742 13 C H5 H 5.25 0.03 1 101 742 13 C H6 H 7.52 0.03 1 102 742 13 C C1' C 90.84 0.1 1 103 742 13 C C2' C 72.64 0.1 1 104 742 13 C C5 C 94.61 0.1 1 105 742 13 C C6 C 137.81 0.1 1 106 743 14 A H1' H 5.92 0.03 1 107 743 14 A H2 H 7.30 0.03 1 108 743 14 A H2' H 4.48 0.03 1 109 743 14 A H8 H 8.00 0.03 1 110 743 14 A C1' C 90.41 0.1 1 111 743 14 A C2 C 150.51 0.1 1 112 743 14 A C2' C 72.82 0.1 1 113 743 14 A C8 C 136.94 0.1 1 114 744 15 U H1' H 5.43 0.03 1 115 744 15 U H2' H 4.39 0.03 1 116 744 15 U H5 H 5.00 0.03 1 117 744 15 U H6 H 7.52 0.03 1 118 744 15 U C1' C 90.52 0.1 1 119 744 15 U C2' C 72.63 0.1 1 120 744 15 U C5 C 100.44 0.1 1 121 744 15 U C6 C 138.13 0.1 1 122 745 16 G H1' H 5.75 0.03 1 123 745 16 G H2' H 4.43 0.03 1 124 745 16 G H3' H 4.43 0.03 1 125 745 16 G H8 H 7.57 0.03 1 126 745 16 G C1' C 89.62 0.1 1 127 745 16 G C2' C 71.04 0.1 1 128 745 16 G C3' C 80.31 0.1 1 129 745 16 G C8 C 133.70 0.1 1 130 746 17 C H1' H 5.62 0.03 1 131 746 17 C H2' H 4.18 0.03 1 132 746 17 C H5 H 5.62 0.03 1 133 746 17 C H6 H 7.71 0.03 1 134 746 17 C C1' C 89.57 0.1 1 135 746 17 C C2' C 73.78 0.1 1 136 746 17 C C5 C 95.67 0.1 1 137 746 17 C C6 C 139.29 0.1 1 138 747 18 U H1' H 5.82 0.03 1 139 747 18 U H2' H 4.38 0.03 1 140 747 18 U H3' H 4.45 0.03 1 141 747 18 U H4' H 4.31 0.03 1 142 747 18 U H5 H 5.81 0.03 1 143 747 18 U H5' H 4.15 0.03 2 144 747 18 U H5'' H 4.03 0.03 2 145 747 18 U H6 H 7.84 0.03 1 146 747 18 U C1' C 87.86 0.1 1 147 747 18 U C2' C 72.83 0.1 1 148 747 18 U C3' C 74.48 0.1 1 149 747 18 U C4' C 82.70 0.1 1 150 747 18 U C5 C 102.47 0.1 1 151 747 18 U C5' C 64.74 0.1 1 152 747 18 U C6 C 140.97 0.1 1 153 748 19 U H1' H 5.53 0.03 1 154 748 19 U H2' H 4.11 0.03 1 155 748 19 U H3' H 4.43 0.03 1 156 748 19 U H4' H 3.80 0.03 1 157 748 19 U H5 H 5.67 0.03 1 158 748 19 U H6 H 7.59 0.03 1 159 748 19 U C1' C 87.18 0.1 1 160 748 19 U C2' C 73.36 0.1 1 161 748 19 U C3' C 74.71 0.1 1 162 748 19 U C4' C 83.11 0.1 1 163 748 19 U C5 C 102.54 0.1 1 164 748 19 U C6 C 140.84 0.1 1 165 749 20 U H1' H 5.87 0.03 1 166 749 20 U H2' H 4.30 0.03 1 167 749 20 U H3' H 4.72 0.03 1 168 749 20 U H4' H 4.39 0.03 1 169 749 20 U H5 H 5.83 0.03 1 170 749 20 U H5' H 3.94 0.03 2 171 749 20 U H5'' H 3.92 0.03 2 172 749 20 U H6 H 7.71 0.03 1 173 749 20 U C1' C 87.38 0.1 1 174 749 20 U C2' C 73.04 0.1 1 175 749 20 U C3' C 74.55 0.1 1 176 749 20 U C4' C 82.44 0.1 1 177 749 20 U C5 C 102.86 0.1 1 178 749 20 U C5' C 65.46 0.1 1 179 749 20 U C6 C 141.21 0.1 1 180 750 21 A H1' H 6.08 0.03 1 181 750 21 A H2 H 8.16 0.03 1 182 750 21 A H2' H 4.88 0.03 1 183 750 21 A H4' H 4.62 0.03 1 184 750 21 A H8 H 8.54 0.03 1 185 750 21 A C1' C 88.85 0.1 1 186 750 21 A C2 C 148.17 0.1 1 187 750 21 A C2' C 72.83 0.1 1 188 750 21 A C4' C 81.36 0.1 1 189 750 21 A C8 C 140.18 0.1 1 190 751 22 C H1' H 5.51 0.03 1 191 751 22 C H2' H 4.51 0.03 1 192 751 22 C H5 H 5.61 0.03 1 193 751 22 C H6 H 7.70 0.03 1 194 751 22 C C1' C 90.82 0.1 1 195 751 22 C C2' C 70.72 0.1 1 196 751 22 C C5 C 95.36 0.1 1 197 751 22 C C6 C 138.84 0.1 1 198 752 23 A H1' H 5.99 0.03 1 199 752 23 A H2 H 7.36 0.03 1 200 752 23 A H2' H 4.53 0.03 1 201 752 23 A H8 H 8.08 0.03 1 202 752 23 A C1' C 90.50 0.1 1 203 752 23 A C2 C 150.52 0.1 1 204 752 23 A C2' C 72.86 0.1 1 205 752 23 A C8 C 137.16 0.1 1 206 753 24 U H1' H 5.46 0.03 1 207 753 24 U H2' H 4.50 0.03 1 208 753 24 U H5 H 5.04 0.03 1 209 753 24 U H6 H 7.55 0.03 1 210 753 24 U C1' C 90.36 0.1 1 211 753 24 U C2' C 72.69 0.1 1 212 753 24 U C5 C 100.32 0.1 1 213 753 24 U C6 C 138.28 0.1 1 214 754 25 G H1' H 5.75 0.03 1 215 754 25 G H2' H 4.46 0.03 1 216 754 25 G H8 H 7.63 0.03 1 217 754 25 G C1' C 90.07 0.1 1 218 754 25 G C2' C 72.66 0.1 1 219 754 25 G C8 C 133.70 0.1 1 220 755 26 U H1' H 5.48 0.03 1 221 755 26 U H2' H 4.59 0.03 1 222 755 26 U H5 H 5.04 0.03 1 223 755 26 U H6 H 7.67 0.03 1 224 755 26 U C1' C 90.72 0.1 1 225 755 26 U C2' C 72.56 0.1 1 226 755 26 U C5 C 100.34 0.1 1 227 755 26 U C6 C 138.56 0.1 1 228 756 27 G H1' H 5.76 0.03 1 229 756 27 G H2' H 4.51 0.03 1 230 756 27 G H8 H 7.57 0.03 1 231 756 27 G C1' C 90.21 0.1 1 232 756 27 G C2' C 72.63 0.1 1 233 756 27 G C8 C 133.40 0.1 1 234 757 28 U H1' H 5.52 0.03 1 235 757 28 U H2' H 4.22 0.03 1 236 757 28 U H4' H 4.35 0.03 1 237 757 28 U H5 H 5.39 0.03 1 238 757 28 U H6 H 7.61 0.03 1 239 757 28 U C1' C 90.08 0.1 1 240 757 28 U C2' C 73.34 0.1 1 241 757 28 U C4' C 80.45 0.1 1 242 757 28 U C5 C 101.63 0.1 1 243 757 28 U C6 C 138.18 0.1 1 244 758 29 U H1' H 5.71 0.03 1 245 758 29 U H2' H 4.19 0.03 1 246 758 29 U H3' H 4.53 0.03 1 247 758 29 U H4' H 4.32 0.03 1 248 758 29 U H5 H 5.62 0.03 1 249 758 29 U H6 H 7.70 0.03 1 250 758 29 U C1' C 88.54 0.1 1 251 758 29 U C2' C 72.85 0.1 1 252 758 29 U C3' C 73.37 0.1 1 253 758 29 U C4' C 81.91 0.1 1 254 758 29 U C5 C 102.16 0.1 1 255 758 29 U C6 C 140.79 0.1 1 256 759 30 U H1' H 5.68 0.03 1 257 759 30 U H2' H 4.19 0.03 1 258 759 30 U H3' H 4.54 0.03 1 259 759 30 U H4' H 4.28 0.03 1 260 759 30 U H5 H 5.66 0.03 1 261 759 30 U H6 H 7.60 0.03 1 262 759 30 U C1' C 88.15 0.1 1 263 759 30 U C2' C 72.99 0.1 1 264 759 30 U C3' C 73.93 0.1 1 265 759 30 U C4' C 82.12 0.1 1 266 759 30 U C5 C 102.30 0.1 1 267 759 30 U C6 C 140.86 0.1 1 268 760 31 A H1' H 5.93 0.03 1 269 760 31 A H2 H 7.93 0.03 1 270 760 31 A H2' H 4.72 0.03 1 271 760 31 A H3' H 4.44 0.03 1 272 760 31 A H4' H 4.46 0.03 1 273 760 31 A H5' H 4.09 0.03 2 274 760 31 A H5'' H 4.07 0.03 2 275 760 31 A H8 H 8.24 0.03 1 276 760 31 A C1' C 87.27 0.1 1 277 760 31 A C2 C 152.28 0.1 1 278 760 31 A C2' C 74.07 0.1 1 279 760 31 A C4' C 82.21 0.1 1 280 760 31 A C8 C 139.10 0.1 1 281 761 32 G H1' H 5.75 0.03 1 282 761 32 G H2' H 4.79 0.03 1 283 761 32 G H3' H 4.59 0.03 1 284 761 32 G H4' H 4.54 0.03 1 285 761 32 G H5' H 4.38 0.03 2 286 761 32 G H5'' H 4.21 0.03 2 287 761 32 G H8 H 7.82 0.03 1 288 761 32 G C1' C 89.10 0.1 1 289 761 32 G C2' C 72.60 0.1 1 290 761 32 G C3' C 72.73 0.1 1 291 761 32 G C4' C 81.22 0.1 1 292 761 32 G C5' C 64.90 0.1 1 293 761 32 G C8 C 136.53 0.1 1 294 762 33 U H1' H 5.64 0.03 1 295 762 33 U H2' H 4.63 0.03 1 296 762 33 U H3' H 4.29 0.03 1 297 762 33 U H4' H 4.49 0.03 1 298 762 33 U H5 H 5.61 0.03 1 299 762 33 U H5' H 4.17 0.03 2 300 762 33 U H5'' H 4.43 0.03 2 301 762 33 U H6 H 7.79 0.03 1 302 762 33 U C1' C 90.26 0.1 1 303 762 33 U C2' C 71.31 0.1 1 304 762 33 U C3' C 72.98 0.1 1 305 762 33 U C4' C 81.03 0.1 1 306 762 33 U C5 C 101.94 0.1 1 307 762 33 U C5' C 63.11 0.1 1 308 762 33 U C6 C 139.47 0.1 1 309 763 34 C H1' H 5.59 0.03 1 310 763 34 C H2' H 4.59 0.03 1 311 763 34 C H5 H 5.77 0.03 1 312 763 34 C H6 H 7.93 0.03 1 313 763 34 C C1' C 90.82 0.1 1 314 763 34 C C2' C 72.76 0.1 1 315 763 34 C C5 C 95.45 0.1 1 316 763 34 C C6 C 139.46 0.1 1 317 764 35 G H1' H 5.67 0.03 1 318 764 35 G H2' H 4.59 0.03 1 319 764 35 G H8 H 7.53 0.03 1 320 764 35 G C1' C 90.24 0.1 1 321 764 35 G C2' C 70.60 0.1 1 322 764 35 G C8 C 133.75 0.1 1 323 765 36 A H1' H 5.92 0.03 1 324 765 36 A H2 H 7.38 0.03 1 325 765 36 A H2' H 4.67 0.03 1 326 765 36 A H8 H 7.77 0.03 1 327 765 36 A C1' C 90.16 0.1 1 328 765 36 A C2 C 150.59 0.1 1 329 765 36 A C2' C 70.09 0.1 1 330 765 36 A C8 C 136.63 0.1 1 331 766 37 G H1' H 5.65 0.03 1 332 766 37 G H8 H 7.23 0.03 1 333 766 37 G C1' C 89.96 0.1 1 334 766 37 G C8 C 133.01 0.1 1 335 767 38 C H1' H 5.52 0.03 1 336 767 38 C H5 H 5.18 0.03 1 337 767 38 C H6 H 7.61 0.03 1 338 767 38 C C1' C 91.26 0.1 1 339 767 38 C C5 C 94.40 0.1 1 340 767 38 C C6 C 138.27 0.1 1 341 768 39 C H5 H 5.50 0.03 1 342 768 39 C H6 H 7.78 0.03 1 343 768 39 C C6 C 138.69 0.1 1 stop_ save_