data_25826 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; solution structure of microRNA 20b pre-element ; _BMRB_accession_number 25826 _BMRB_flat_file_name bmr25826.str _Entry_type original _Submission_date 2015-09-25 _Accession_date 2015-09-25 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Yang Fan . . 2 Chen Yu . . 3 Varani Gabriele . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 175 "13C chemical shifts" 153 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2016-07-22 update BMRB 'update entry citation' 2016-04-12 original author 'original release' stop_ loop_ _Related_BMRB_accession_number _Relationship 25831 'complex of microRNA 20b pre-element with Rbfox RRM' stop_ _Original_release_date 2016-04-12 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Rbfox proteins regulate microRNA biogenesis by sequence-specific binding to their precursors and target downstream Dicer ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 27001519 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Chen Yu . . 2 Zubovic Lorena . . 3 Yang Fan . . 4 Godin Katherine . . 5 Pavelitz Tom . . 6 Castellanos Javier . . 7 Macchi Paolo . . 8 Varani Gabriele . . stop_ _Journal_abbreviation 'Nucleic Acids Res.' _Journal_volume 44 _Journal_issue 9 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 4381 _Page_last 4395 _Year 2016 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'microRNA 20b pre-element' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label miR-20b $miR-20b stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_miR-20b _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common miR-20b _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 23 _Mol_residue_sequence ; GGUAGUUUUGGCAUGACUCU ACC ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 19 G 2 20 G 3 21 U 4 22 A 5 23 G 6 24 U 7 25 U 8 26 U 9 27 U 10 28 G 11 29 G 12 30 C 13 31 A 14 32 U 15 33 G 16 34 A 17 35 C 18 36 U 19 37 C 20 38 U 21 39 A 22 40 C 23 41 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $miR-20b human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_type _Vector_name $miR-20b 'T7 in vitro transcription' . . . . NA NA stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $miR-20b 1 mM 'natural abundance' 'sodium phosphate' 10 mM 'natural abundance' 'sodium chloride' 20 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $miR-20b 1 mM 'natural abundance' 'sodium phosphate' 10 mM 'natural abundance' 'sodium chloride' 20 mM 'natural abundance' D2O 100 % 'natural abundance' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $miR-20b 1 mM '[U-99% 13C; U-99% 15N]' 'sodium phosphate' 10 mM 'natural abundance' 'sodium chloride' 20 mM 'natural abundance' D2O 100 % 'natural abundance' stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $miR-20b 1 mM '[U-99% 13C; U-99% 15N]' 'sodium phosphate' 10 mM 'natural abundance' 'sodium chloride' 20 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection stop_ _Details . save_ save_CYANA _Saveframe_category software _Name CYANA _Version . loop_ _Vendor _Address _Electronic_address 'Guntert, Mumenthaler and Wuthrich' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ save_CcpNMR _Saveframe_category software _Name CcpNMR _Version . loop_ _Vendor _Address _Electronic_address CCPN . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' 'peak picking' stop_ _Details . save_ save_AMBER _Saveframe_category software _Name AMBER _Version . loop_ _Vendor _Address _Electronic_address 'Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman' . . stop_ loop_ _Task refinement stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_TALOS _Saveframe_category software _Name TALOS _Version . loop_ _Vendor _Address _Electronic_address 'Cornilescu, Delaglio and Bax' . . stop_ loop_ _Task 'geometry optimization' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_2D_1H-1H_TOCSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_2 save_ save_2D_1H-13C_HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_3 save_ save_3D_1H-13C_NOESY_5 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_3 save_ save_2D_1H-15N_HSQC_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_4 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 . pH pressure 1 . atm temperature 279 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 . pH pressure 1 . atm temperature 298 . K stop_ save_ save_sample_conditions_3 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 . pH pressure 1 . atm temperature 298 . K stop_ save_ save_sample_conditions_4 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 . pH pressure 1 . atm temperature 279 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' '2D 1H-1H TOCSY' '2D 1H-13C HSQC' '3D 1H-13C NOESY' stop_ loop_ _Sample_label $sample_2 $sample_3 stop_ _Sample_conditions_label $sample_conditions_2 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name miR-20b _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 19 1 G H1' H 5.827 0.001 1 2 19 1 G H2' H 4.955 0.003 2 3 19 1 G H3' H 4.703 0.004 1 4 19 1 G H4' H 4.554 0.002 1 5 19 1 G H5' H 4.266 0.003 2 6 19 1 G H5'' H 4.417 0.005 2 7 19 1 G H8 H 8.128 0.003 1 8 19 1 G C1' C 91.546 0.029 1 9 19 1 G C2' C 75.120 0.024 1 10 19 1 G C3' C 75.836 0.028 1 11 19 1 G C4' C 83.461 0.024 1 12 19 1 G C5' C 67.346 0.003 1 13 19 1 G C8 C 139.163 0.021 1 14 20 2 G H1' H 5.912 0.003 1 15 20 2 G H2' H 4.535 0.005 2 16 20 2 G H4' H 4.546 0.003 1 17 20 2 G H5' H 4.250 0.004 2 18 20 2 G H5'' H 4.499 0.009 2 19 20 2 G H8 H 7.544 0.002 1 20 20 2 G C1' C 93.108 0.044 1 21 20 2 G C2' C 75.372 0.005 1 22 20 2 G C4' C 82.664 0.015 1 23 20 2 G C5' C 66.305 0.004 1 24 20 2 G C8 C 137.063 0.025 1 25 21 3 U H1' H 5.539 0.009 1 26 21 3 U H2' H 4.508 0.002 1 27 21 3 U H3' H 4.589 0.006 1 28 21 3 U H4' H 4.465 0.002 1 29 21 3 U H5 H 5.159 0.004 1 30 21 3 U H5' H 4.137 0.003 2 31 21 3 U H5'' H 4.584 0.010 2 32 21 3 U H6 H 7.763 0.005 1 33 21 3 U C1' C 93.651 0.029 1 34 21 3 U C2' C 75.373 0.009 1 35 21 3 U C3' C 72.152 0.014 1 36 21 3 U C4' C 82.199 0.013 1 37 21 3 U C5 C 103.000 0.010 1 38 21 3 U C5' C 64.510 0.002 1 39 21 3 U C6 C 141.441 0.010 1 40 22 4 A H1' H 6.038 0.002 1 41 22 4 A H2 H 6.903 0.001 1 42 22 4 A H2' H 4.742 0.004 2 43 22 4 A H3' H 4.733 0.006 1 44 22 4 A H4' H 4.500 0.006 1 45 22 4 A H5' H 4.182 0.004 2 46 22 4 A H5'' H 4.566 0.006 2 47 22 4 A H8 H 8.103 0.002 1 48 22 4 A C1' C 92.472 0.050 1 49 22 4 A C2 C 152.966 0.000 1 50 22 4 A C2' C 75.983 0.016 1 51 22 4 A C3' C 73.101 0.035 1 52 22 4 A C4' C 81.987 0.002 1 53 22 4 A C5' C 65.172 0.001 1 54 22 4 A C8 C 139.560 0.044 1 55 23 5 G H1' H 5.421 0.002 1 56 23 5 G H2' H 4.482 0.004 2 57 23 5 G H3' H 4.048 0.006 1 58 23 5 G H4' H 4.465 0.009 1 59 23 5 G H5' H 4.039 0.003 2 60 23 5 G H5'' H 4.339 0.009 2 61 23 5 G H8 H 7.012 0.005 1 62 23 5 G C1' C 92.750 0.018 1 63 23 5 G C2' C 75.391 0.025 1 64 23 5 G C3' C 73.638 0.022 1 65 23 5 G C4' C 82.344 0.000 1 66 23 5 G C5' C 67.308 0.007 1 67 23 5 G C8 C 135.585 0.021 1 68 24 6 U H1' H 5.370 0.001 1 69 24 6 U H2' H 4.180 0.004 1 70 24 6 U H3' H 4.352 0.007 1 71 24 6 U H4' H 4.356 0.001 1 72 24 6 U H5 H 5.180 0.005 1 73 24 6 U H5' H 4.027 0.009 2 74 24 6 U H5'' H 4.416 0.006 2 75 24 6 U H6 H 7.459 0.003 1 76 24 6 U C1' C 94.272 0.054 1 77 24 6 U C2' C 75.098 0.023 1 78 24 6 U C3' C 72.141 0.022 1 79 24 6 U C4' C 82.591 0.000 1 80 24 6 U C5 C 103.655 0.031 1 81 24 6 U C5' C 64.455 0.007 1 82 24 6 U C6 C 140.692 0.013 1 83 25 7 U H1' H 5.569 0.007 1 84 25 7 U H2' H 4.090 0.003 1 85 25 7 U H3' H 4.556 0.007 1 86 25 7 U H4' H 4.322 0.004 1 87 25 7 U H5 H 5.487 0.004 1 88 25 7 U H5' H 4.031 0.003 2 89 25 7 U H5'' H 4.526 0.008 2 90 25 7 U H6 H 7.977 0.004 1 91 25 7 U C1' C 93.316 0.027 1 92 25 7 U C2' C 75.601 0.020 1 93 25 7 U C3' C 72.381 0.011 1 94 25 7 U C4' C 82.281 0.000 1 95 25 7 U C5 C 103.018 0.009 1 96 25 7 U C5' C 64.272 0.000 1 97 25 7 U C6 C 142.770 0.063 1 98 26 8 U H1' H 5.784 0.001 1 99 26 8 U H2' H 4.653 0.005 1 100 26 8 U H3' H 4.465 0.006 1 101 26 8 U H4' H 4.362 0.004 1 102 26 8 U H5 H 5.606 0.002 1 103 26 8 U H6 H 7.970 0.002 1 104 26 8 U C1' C 92.815 0.019 1 105 26 8 U C2' C 75.414 0.014 1 106 26 8 U C3' C 72.940 0.023 1 107 26 8 U C4' C 82.186 0.007 1 108 26 8 U C5 C 103.108 0.022 1 109 26 8 U C6 C 142.612 0.038 1 110 27 9 U H1' H 5.557 0.006 1 111 27 9 U H2' H 4.338 0.007 1 112 27 9 U H3' H 4.359 0.007 1 113 27 9 U H4' H 4.426 0.004 1 114 27 9 U H5 H 5.651 0.003 1 115 27 9 U H5' H 4.112 0.005 2 116 27 9 U H5'' H 4.361 0.007 2 117 27 9 U H6 H 7.684 0.004 1 118 27 9 U C1' C 94.303 0.068 1 119 27 9 U C2' C 75.578 0.015 1 120 27 9 U C3' C 73.978 0.024 1 121 27 9 U C4' C 83.332 0.033 1 122 27 9 U C5 C 105.264 0.025 1 123 27 9 U C5' C 66.838 0.002 1 124 27 9 U C6 C 141.440 0.027 1 125 28 10 G H1' H 5.567 0.002 1 126 28 10 G H2' H 4.494 0.004 2 127 28 10 G H3' H 4.706 0.003 1 128 28 10 G H4' H 4.375 0.003 1 129 28 10 G H5' H 4.068 0.004 2 130 28 10 G H5'' H 4.334 0.007 2 131 28 10 G H8 H 7.892 0.004 1 132 28 10 G C1' C 90.641 0.035 1 133 28 10 G C2' C 75.684 0.013 1 134 28 10 G C3' C 76.097 0.000 1 135 28 10 G C4' C 84.229 0.022 1 136 28 10 G C5' C 66.481 0.007 1 137 28 10 G C8 C 138.622 0.008 1 138 29 11 G H1' H 5.597 0.003 1 139 29 11 G H2' H 4.675 0.005 2 140 29 11 G H3' H 4.624 0.004 1 141 29 11 G H4' H 4.325 0.001 1 142 29 11 G H5' H 4.063 0.006 2 143 29 11 G H5'' H 4.189 0.004 2 144 29 11 G H8 H 7.850 0.004 1 145 29 11 G C1' C 89.593 0.019 1 146 29 11 G C2' C 75.287 0.026 1 147 29 11 G C3' C 77.061 0.017 1 148 29 11 G C4' C 85.029 0.016 1 149 29 11 G C5' C 67.535 0.005 1 150 29 11 G C8 C 140.205 0.028 1 151 30 12 C H1' H 5.766 0.004 1 152 30 12 C H2' H 4.107 0.004 2 153 30 12 C H3' H 4.514 0.005 1 154 30 12 C H4' H 4.107 0.001 1 155 30 12 C H5 H 5.706 0.003 1 156 30 12 C H5' H 3.751 0.002 2 157 30 12 C H5'' H 3.919 0.005 2 158 30 12 C H6 H 7.536 0.002 1 159 30 12 C C1' C 90.770 0.004 1 160 30 12 C C2' C 76.719 0.022 1 161 30 12 C C3' C 77.251 0.022 1 162 30 12 C C4' C 84.645 0.030 1 163 30 12 C C5 C 98.826 0.021 1 164 30 12 C C5' C 67.527 0.000 1 165 30 12 C C6 C 143.295 0.027 1 166 31 13 A H1' H 5.914 0.003 1 167 31 13 A H2 H 7.945 0.002 1 168 31 13 A H2' H 4.836 0.007 2 169 31 13 A H3' H 4.822 0.007 1 170 31 13 A H4' H 4.451 0.002 1 171 31 13 A H5' H 4.102 0.004 2 172 31 13 A H5'' H 4.228 0.006 2 173 31 13 A H8 H 8.221 0.001 1 174 31 13 A C1' C 90.356 0.054 1 175 31 13 A C2 C 155.073 0.000 1 176 31 13 A C2' C 76.055 0.014 1 177 31 13 A C3' C 77.056 0.010 1 178 31 13 A C4' C 85.274 0.035 1 179 31 13 A C5' C 68.114 0.014 1 180 31 13 A C8 C 142.352 0.018 1 181 32 14 U H1' H 5.935 0.003 1 182 32 14 U H2' H 4.449 0.003 1 183 32 14 U H3' H 4.678 0.003 1 184 32 14 U H4' H 4.520 0.004 1 185 32 14 U H5 H 5.811 0.002 1 186 32 14 U H5' H 4.222 0.007 2 187 32 14 U H5'' H 4.269 0.008 2 188 32 14 U H6 H 7.817 0.003 1 189 32 14 U C1' C 90.852 0.032 1 190 32 14 U C2' C 75.649 0.004 1 191 32 14 U C3' C 76.699 0.021 1 192 32 14 U C4' C 85.067 0.042 1 193 32 14 U C5 C 105.303 0.043 1 194 32 14 U C5' C 67.970 0.001 1 195 32 14 U C6 C 143.775 0.011 1 196 33 15 G H1' H 5.177 0.004 1 197 33 15 G H2' H 4.684 0.006 2 198 33 15 G H3' H 4.488 0.003 1 199 33 15 G H4' H 4.491 0.004 1 200 33 15 G H5' H 4.212 0.003 2 201 33 15 G H5'' H 4.366 0.008 2 202 33 15 G H8 H 7.813 0.003 1 203 33 15 G C1' C 92.521 0.014 1 204 33 15 G C2' C 75.161 0.031 1 205 33 15 G C3' C 74.557 0.031 1 206 33 15 G C4' C 83.142 0.059 1 207 33 15 G C5' C 67.800 0.038 1 208 33 15 G C8 C 138.362 0.072 1 209 34 16 A H1' H 5.903 0.002 1 210 34 16 A H2 H 7.971 0.002 1 211 34 16 A H2' H 4.595 0.001 2 212 34 16 A H3' H 4.482 0.006 1 213 34 16 A H4' H 4.482 0.010 1 214 34 16 A H5' H 4.147 0.009 2 215 34 16 A H5'' H 4.470 0.000 2 216 34 16 A H8 H 7.819 0.003 1 217 34 16 A C1' C 92.797 0.028 1 218 34 16 A C2 C 154.188 0.030 1 219 34 16 A C2' C 75.508 0.035 1 220 34 16 A C3' C 72.707 0.014 1 221 34 16 A C4' C 82.313 0.010 1 222 34 16 A C5' C 65.263 0.003 1 223 34 16 A C8 C 138.938 0.088 1 224 35 17 C H1' H 5.283 0.006 1 225 35 17 C H2' H 4.039 0.004 2 226 35 17 C H3' H 4.290 0.011 1 227 35 17 C H4' H 4.321 0.010 1 228 35 17 C H5 H 5.152 0.004 1 229 35 17 C H5' H 4.012 0.007 2 230 35 17 C H5'' H 4.440 0.008 2 231 35 17 C H6 H 7.155 0.004 1 232 35 17 C C1' C 93.586 0.011 1 233 35 17 C C2' C 75.724 0.026 1 234 35 17 C C3' C 72.274 0.024 1 235 35 17 C C4' C 81.914 0.000 1 236 35 17 C C5 C 96.481 0.035 1 237 35 17 C C5' C 64.366 0.008 1 238 35 17 C C6 C 140.268 0.023 1 239 36 18 U H1' H 5.523 0.004 1 240 36 18 U H2' H 4.313 0.004 1 241 36 18 U H3' H 4.481 0.007 1 242 36 18 U H4' H 4.338 0.000 1 243 36 18 U H5 H 5.354 0.003 1 244 36 18 U H5' H 4.448 0.005 2 245 36 18 U H5'' H 4.049 0.006 2 246 36 18 U H6 H 7.783 0.004 1 247 36 18 U C1' C 93.766 0.026 1 248 36 18 U C2' C 75.435 0.024 1 249 36 18 U C3' C 72.384 0.064 1 250 36 18 U C4' C 82.267 0.000 1 251 36 18 U C5 C 103.362 0.017 1 252 36 18 U C5' C 64.320 0.011 1 253 36 18 U C6 C 142.407 0.023 1 254 37 19 C H1' H 5.652 0.003 1 255 37 19 C H2' H 4.391 0.004 2 256 37 19 C H3' H 4.510 0.008 1 257 37 19 C H4' H 4.402 0.007 1 258 37 19 C H5 H 5.871 0.004 1 259 37 19 C H5' H 4.103 0.008 2 260 37 19 C H5'' H 4.426 0.006 2 261 37 19 C H6 H 7.932 0.002 1 262 37 19 C C1' C 92.895 0.012 1 263 37 19 C C2' C 75.633 0.013 1 264 37 19 C C3' C 73.638 0.057 1 265 37 19 C C4' C 82.320 0.016 1 266 37 19 C C5 C 98.290 0.033 1 267 37 19 C C5' C 65.457 0.008 1 268 37 19 C C6 C 142.241 0.050 1 269 38 20 U H1' H 5.514 0.004 1 270 38 20 U H2' H 4.611 0.008 1 271 38 20 U H3' H 4.529 0.006 1 272 38 20 U H4' H 4.505 0.003 1 273 38 20 U H5 H 5.525 0.002 1 274 38 20 U H5' H 4.190 0.013 2 275 38 20 U H5'' H 4.496 0.009 2 276 38 20 U H6 H 7.814 0.004 1 277 38 20 U C1' C 94.119 0.058 1 278 38 20 U C2' C 75.075 0.049 1 279 38 20 U C3' C 73.238 0.029 1 280 38 20 U C4' C 82.479 0.000 1 281 38 20 U C5 C 103.929 0.016 1 282 38 20 U C5' C 66.077 0.000 1 283 38 20 U C6 C 142.456 0.033 1 284 39 21 A H1' H 6.025 0.002 1 285 39 21 A H2 H 7.093 0.002 1 286 39 21 A H2' H 4.509 0.002 2 287 39 21 A H3' H 4.725 0.003 1 288 39 21 A H4' H 4.508 0.003 1 289 39 21 A H5' H 4.610 0.003 2 290 39 21 A H5'' H 4.180 0.004 2 291 39 21 A H8 H 8.277 0.002 1 292 39 21 A C1' C 92.869 0.028 1 293 39 21 A C2 C 153.363 0.000 1 294 39 21 A C2' C 75.517 0.029 1 295 39 21 A C3' C 72.670 0.079 1 296 39 21 A C4' C 82.058 0.057 1 297 39 21 A C5' C 64.927 0.008 1 298 39 21 A C8 C 139.956 0.030 1 299 40 22 C H1' H 5.376 0.003 1 300 40 22 C H2' H 4.057 0.005 2 301 40 22 C H3' H 4.357 0.004 1 302 40 22 C H4' H 4.355 0.005 1 303 40 22 C H5 H 5.203 0.003 1 304 40 22 C H5' H 4.036 0.004 2 305 40 22 C H5'' H 4.512 0.004 2 306 40 22 C H6 H 7.544 0.004 1 307 40 22 C C1' C 93.856 0.030 1 308 40 22 C C2' C 75.762 0.011 1 309 40 22 C C3' C 72.112 0.008 1 310 40 22 C C4' C 81.950 0.000 1 311 40 22 C C5 C 96.940 0.027 1 312 40 22 C C5' C 64.361 0.002 1 313 40 22 C C6 C 140.884 0.021 1 314 41 23 C H1' H 5.733 0.001 1 315 41 23 C H2' H 3.976 0.003 2 316 41 23 C H3' H 4.140 0.002 1 317 41 23 C H4' H 4.132 0.005 1 318 41 23 C H5 H 5.423 0.003 1 319 41 23 C H5' H 3.982 0.008 2 320 41 23 C H5'' H 4.431 0.006 2 321 41 23 C H6 H 7.611 0.006 1 322 41 23 C C1' C 92.777 0.006 1 323 41 23 C C2' C 77.548 0.010 1 324 41 23 C C3' C 69.835 0.014 1 325 41 23 C C4' C 83.539 0.012 1 326 41 23 C C5 C 98.106 0.017 1 327 41 23 C C5' C 65.232 0.002 1 328 41 23 C C6 C 141.890 0.013 1 stop_ save_