data_25121 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; The influenza hemagglutinin fusion domain is an amphipathic helical-hairpin that functions by inducing negative membrane curvature ; _BMRB_accession_number 25121 _BMRB_flat_file_name bmr25121.str _Entry_type original _Submission_date 2014-07-31 _Accession_date 2014-07-31 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Smrt Sean T. . 2 Draney Adrian W. . 3 Lorieau Justin L. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count H_exch_rates 1 assigned_chemical_shifts 2 heteronucl_NOE 2 T1_relaxation 2 T2_relaxation 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 48 "15N chemical shifts" 48 "T1 relaxation values" 42 "T2 relaxation values" 42 "H exchange rates" 21 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2019-07-11 original BMRB . stop_ _Original_release_date 2014-08-01 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; The influenza hemagglutinin fusion domain is an amphipathic helical hairpin that functions by inducing membrane curvature ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 25398882 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Smrt Sean T. . 2 Draney Adrian W. . 3 Lorieau Justin L. . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_volume 290 _Journal_issue 1 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 228 _Page_last 238 _Year 2015 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Influenza Hemagluttinin Fusion Peptide' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label HAfp23-F3G $HAfp23-F3G stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_HAfp23-F3G _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common HAfp23-F3G _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 30 _Mol_residue_sequence ; GLGGAIAGFIEGGWTGMIDG WYGGSKKKKD ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 1 GLY 2 2 LEU 3 3 GLY 4 4 GLY 5 5 ALA 6 6 ILE 7 7 ALA 8 8 GLY 9 9 PHE 10 10 ILE 11 11 GLU 12 12 GLY 13 13 GLY 14 14 TRP 15 15 THR 16 16 GLY 17 17 MET 18 18 ILE 19 19 ASP 20 20 GLY 21 21 TRP 22 22 TYR 23 23 GLY 24 24 GLY 25 25 SER 26 26 LYS 27 27 LYS 28 28 LYS 29 29 LYS 30 30 ASP stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $HAfp23-F3G 'Influenza Virus Type A' 11320 Viruses . 'Influenzavirus A' . stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $HAfp23-F3G 'recombinant technology' . Escherichia coli . pET15b stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_pH_7.4_sample _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $HAfp23-F3G 850 uM '[U-100% 15N]' TRIS 25 mM 'natural abundance' DPC 150 mM 'natural abundance' D2O 10 % 'natural abundance' H2O 90 % 'natural abundance' stop_ save_ save_pH_4.2_sample _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $HAfp23-F3G 600 uM '[U-100% 15N]' Citrate 25 mM 'natural abundance' DPC 150 mM 'natural abundance' D2O 10 % 'natural abundance' H2O 90 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' 'peak picking' stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task 'data analysis' processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $pH_7.4_sample save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $pH_4.2_sample save_ ####################### # Sample conditions # ####################### save_pH_7.4_Sample_condition _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0 . M pH 7.4 . pH pressure 1 . atm temperature 305 . K stop_ save_ save_pH_4.2_Sample_condition _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0 . M pH 4.2 . pH pressure 1 . atm temperature 305 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio water H 1 protons ppm 4.7 internal direct . . . 1 na N 15 nitrogen ppm 0 internal indirect . . . 0.1013 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_pH_7.4 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $pH_7.4_sample stop_ _Sample_conditions_label $pH_7.4_Sample_condition _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name HAfp23-F3G _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 LEU H H 9.794 . . 2 2 2 LEU N N 126.238 . . 3 3 3 GLY H H 9.184 . . 4 3 3 GLY N N 106.431 . . 5 4 4 GLY H H 8.498 . . 6 4 4 GLY N N 109.07 . . 7 5 5 ALA H H 8.426 . . 8 5 5 ALA N N 126.456 . . 9 6 6 ILE H H 8.081 . . 10 6 6 ILE N N 118.335 . . 11 7 7 ALA H H 8.167 . . 12 7 7 ALA N N 120.068 . . 13 8 8 GLY H H 8.353 . . 14 8 8 GLY N N 105.519 . . 15 9 9 PHE H H 8.305 . . 16 9 9 PHE N N 122.983 . . 17 10 10 ILE H H 7.885 . . 18 10 10 ILE N N 119.032 . . 19 11 11 GLU H H 7.773 . . 20 11 11 GLU N N 115.351 . . 21 12 12 GLY H H 8.433 . . 22 12 12 GLY N N 105.299 . . 23 13 13 GLY H H 8.365 . . 24 13 13 GLY N N 112.051 . . 25 14 14 TRP H H 9.054 . . 26 14 14 TRP HE1 H 10.736 . . 27 14 14 TRP N N 124.361 . . 28 14 14 TRP NE1 N 130.598 . . 29 15 15 THR H H 8.585 . . 30 15 15 THR N N 110.617 . . 31 16 16 GLY H H 7.23 . . 32 16 16 GLY N N 108.935 . . 33 17 17 MET H H 8.502 . . 34 17 17 MET N N 124.743 . . 35 18 18 ILE H H 7.816 . . 36 18 18 ILE N N 119.297 . . 37 19 19 ASP H H 7.978 . . 38 19 19 ASP N N 118.53 . . 39 20 20 GLY H H 8.353 . . 40 20 20 GLY N N 107.146 . . 41 21 21 TRP H H 8.544 . . 42 21 21 TRP HE1 H 10.359 . . 43 21 21 TRP N N 123.706 . . 44 21 21 TRP NE1 N 129.845 . . 45 22 22 TYR H H 7.658 . . 46 22 22 TYR N N 113.207 . . 47 23 23 GLY H H 7.959 . . 48 23 23 GLY N N 107.912 . . stop_ save_ save_assigned_chem_shift_list_pH_4.2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label $pH_4.2_sample stop_ _Sample_conditions_label $pH_4.2_Sample_condition _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name HAfp23-F3G _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 LEU H H 9.785 . . 2 2 2 LEU N N 125.469 . . 3 3 3 GLY H H 9.203 . . 4 3 3 GLY N N 105.89 . . 5 4 4 GLY H H 8.52 . . 6 4 4 GLY N N 108.134 . . 7 5 5 ALA H H 8.382 . . 8 5 5 ALA N N 125.428 . . 9 6 6 ILE H H 8.082 . . 10 6 6 ILE N N 117.637 . . 11 7 7 ALA H H 8.235 . . 12 7 7 ALA N N 119.43 . . 13 8 8 GLY H H 8.296 . . 14 8 8 GLY N N 103.946 . . 15 9 9 PHE H H 8.237 . . 16 9 9 PHE N N 121.963 . . 17 10 10 ILE H H 7.895 . . 18 10 10 ILE N N 118.043 . . 19 11 11 GLU H H 8 . . 20 11 11 GLU N N 113.324 . . 21 12 12 GLY H H 8.343 . . 22 12 12 GLY N N 105.055 . . 23 13 13 GLY H H 8.278 . . 24 13 13 GLY N N 110.948 . . 25 14 14 TRP H H 8.969 . . 26 14 14 TRP HE1 H 10.68 . . 27 14 14 TRP N N 123.246 . . 28 14 14 TRP NE1 N 129.807 . . 29 15 15 THR H H 8.539 . . 30 15 15 THR N N 110.631 . . 31 16 16 GLY H H 7.312 . . 32 16 16 GLY N N 108.075 . . 33 17 17 MET H H 8.408 . . 34 17 17 MET N N 123.557 . . 35 18 18 ILE H H 7.882 . . 36 18 18 ILE N N 118.441 . . 37 19 19 ASP H H 8.114 . . 38 19 19 ASP N N 117.452 . . 39 20 20 GLY H H 8.325 . . 40 20 20 GLY N N 106.731 . . 41 21 21 TRP H H 8.467 . . 42 21 21 TRP HE1 H 10.361 . . 43 21 21 TRP N N 122.702 . . 44 21 21 TRP NE1 N 129.122 . . 45 22 22 TYR H H 7.704 . . 46 22 22 TYR N N 112.897 . . 47 23 23 GLY H H 7.927 . . 48 23 23 GLY N N 107.093 . . stop_ save_ save_heteronuclear_T1_pH7 _Saveframe_category T1_relaxation _Details . loop_ _Sample_label $pH_7.4_sample stop_ _Sample_conditions_label $pH_7.4_Sample_condition _Spectrometer_frequency_1H 500 _T1_coherence_type Nz _T1_value_units s _Mol_system_component_name HAfp23-F3G _Text_data_format . _Text_data . loop_ _T1_ID _Residue_seq_code _Residue_label _Atom_name _T1_value _T1_value_error 1 3 GLY N 0.6292 0.00828 2 4 GLY N 0.6272 0.00991 3 5 ALA N 0.6107 0.00944 4 6 ILE N 0.6102 0.00413 5 7 ALA N 0.5915 0.00463 6 8 GLY N 0.6015 0.00741 7 9 PHE N 0.6312 0.00767 8 10 ILE N 0.5829 0.00142 9 11 GLU N 0.6009 0.00437 10 12 GLY N 0.6361 0.00343 11 13 GLY N 0.6092 0.01020 12 14 TRP N 0.6321 0.01490 13 15 THR N 0.6448 0.00877 14 16 GLY N 0.6130 0.00423 15 17 MET N 0.5956 0.00541 16 18 ILE N 0.5898 0.00725 17 19 ASP N 0.6086 0.00437 18 20 GLY N 0.6030 0.01010 19 21 TRP N 0.6098 0.00600 20 22 TYR N 0.6242 0.01840 21 23 GLY N 0.6813 0.02240 stop_ save_ save_heteronuclear_T1_pH4.2 _Saveframe_category T1_relaxation _Details . loop_ _Sample_label $pH_4.2_sample stop_ _Sample_conditions_label $pH_4.2_Sample_condition _Spectrometer_frequency_1H 500 _T1_coherence_type Nz _T1_value_units s _Mol_system_component_name HAfp23-F3G _Text_data_format . _Text_data . loop_ _T1_ID _Residue_seq_code _Residue_label _Atom_name _T1_value _T1_value_error 1 3 GLY N 0.5891 0.024320047 2 4 GLY N 0.5987 0.032641292 3 5 ALA N 0.6025 0.030577986 4 6 ILE N 0.6208 0.025091294 5 7 ALA N 0.5880 0.045698552 6 8 GLY N 0.6227 0.012121047 7 9 PHE N 0.6390 0.013420814 8 10 ILE N 0.5984 0.020581846 9 11 GLU N 0.6255 0.022773148 10 12 GLY N 0.6637 0.021180588 11 13 GLY N 0.6064 0.024855804 12 14 TRP N 0.6278 0.022251381 13 15 THR N 0.6337 0.047313621 14 16 GLY N 0.6272 0.024454736 15 17 MET N 0.6085 0.025818840 16 18 ILE N 0.5891 0.007751294 17 19 ASP N 0.5889 0.017502722 18 20 GLY N 0.6178 0.022636951 19 21 TRP N 0.6101 0.033313452 20 22 TYR N 0.5788 0.044774858 21 23 GLY N 0.6460 0.033547719 stop_ save_ save_heteronuclear_T2_pH7.4 _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $pH_7.4_sample stop_ _Sample_conditions_label $pH_7.4_Sample_condition _Spectrometer_frequency_1H 500 _T2_coherence_type Nz _T2_value_units s _Mol_system_component_name HAfp23-F3G _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 3 GLY N 0.0924 0.0028 . . 2 4 GLY N 0.0954 0.0029 . . 3 5 ALA N 0.0895 0.0027 . . 4 6 ILE N 0.0944 0.0028 . . 5 7 ALA N 0.0931 0.0028 . . 6 8 GLY N 0.0890 0.0027 . . 7 9 PHE N 0.0968 0.0029 . . 8 10 ILE N 0.0942 0.0028 . . 9 11 GLU N 0.0888 0.0027 . . 10 12 GLY N 0.0966 0.0029 . . 11 13 GLY N 0.0942 0.0028 . . 12 14 TRP N 0.0960 0.0029 . . 13 15 THR N 0.0978 0.0029 . . 14 16 GLY N 0.0940 0.0028 . . 15 17 MET N 0.0862 0.0026 . . 16 18 ILE N 0.0922 0.0028 . . 17 19 ASP N 0.0922 0.0028 . . 18 20 GLY N 0.0895 0.0027 . . 19 21 TRP N 0.0939 0.0028 . . 20 22 TYR N 0.0957 0.0029 . . 21 23 GLY N 0.1186 0.0036 . . stop_ save_ save_heteronuclear_T2_pH4.2 _Saveframe_category T2_relaxation _Details . loop_ _Sample_label $pH_4.2_sample stop_ _Sample_conditions_label $pH_4.2_Sample_condition _Spectrometer_frequency_1H 500 _T2_coherence_type Nz _T2_value_units s _Mol_system_component_name HAfp23-F3G _Text_data_format . _Text_data . loop_ _T2_ID _Residue_seq_code _Residue_label _Atom_name _T2_value _T2_value_error _Rex_value _Rex_error 1 3 GLY N 0.0955 0.0030 . . 2 4 GLY N 0.0941 0.0030 . . 3 5 ALA N 0.0846 0.0028 . . 4 6 ILE N 0.0881 0.0030 . . 5 7 ALA N 0.0874 0.0030 . . 6 8 GLY N 0.0869 0.0025 . . 7 9 PHE N 0.0819 0.0027 . . 8 10 ILE N 0.0904 0.0031 . . 9 11 GLU N 0.0792 0.0026 . . 10 12 GLY N 0.0937 0.0028 . . 11 13 GLY N 0.0816 0.0026 . . 12 14 TRP N 0.0868 0.0029 . . 13 15 THR N 0.0919 0.0030 . . 14 16 GLY N 0.0923 0.0029 . . 15 17 MET N 0.0732 0.0024 . . 16 18 ILE N 0.0907 0.0031 . . 17 19 ASP N 0.0886 0.0031 . . 18 20 GLY N 0.0928 0.0031 . . 19 21 TRP N 0.0836 0.0028 . . 20 22 TYR N 0.0857 0.0029 . . 21 23 GLY N 0.1191 0.0039 . . stop_ save_ save_heteronuclear_noe_pH7.4 _Saveframe_category heteronuclear_NOE _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label . stop_ _Sample_conditions_label $pH_7.4_Sample_condition _Spectrometer_frequency_1H 500 _Mol_system_component_name HAfp23-F3G _Atom_one_atom_name N _Atom_two_atom_name H _Heteronuclear_NOE_value_type 'peak height' _NOE_reference_value 1 _NOE_reference_description . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Heteronuclear_NOE_value _Heteronuclear_NOE_value_error 3 GLY 0.688235294 0.012738511 4 GLY 0.639573618 0.012747050 5 ALA 0.712643678 0.009423380 6 ILE 0.684782609 0.010578250 7 ALA 0.694052419 0.009845687 8 GLY 0.695269527 0.010749762 9 PHE 0.548938135 0.008551965 10 ILE 0.660835214 0.010884155 11 GLU 0.650275966 0.009638768 12 GLY 0.619565217 0.010321993 13 GLY 0.675186368 0.010326085 14 TRP 0.689425982 0.011781806 15 THR 0.661572052 0.008424500 16 GLY 0.715605749 0.010112382 17 MET 0.671700224 0.010831411 18 ILE 0.663282572 0.010888111 19 ASP 0.701713756 0.009074619 20 GLY 0.684514436 0.010216257 21 TRP 0.648111332 0.009539993 22 TYR 0.664647577 0.010635808 23 GLY 0.487461505 0.007986476 stop_ save_ save_heteronuclear_noe_pH4.2 _Saveframe_category heteronuclear_NOE _Details . loop_ _Experiment_label '2D 1H-15N HSQC' stop_ loop_ _Sample_label . stop_ _Sample_conditions_label $pH_4.2_Sample_condition _Spectrometer_frequency_1H 500 _Mol_system_component_name HAfp23-F3G _Atom_one_atom_name N _Atom_two_atom_name H _Heteronuclear_NOE_value_type 'peak height' _NOE_reference_value 1 _NOE_reference_description . _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Heteronuclear_NOE_value _Heteronuclear_NOE_value_error 3 GLY 0.6882 0.00954 4 GLY 0.6396 0.00973 5 ALA 0.7126 0.00699 6 ILE 0.6848 0.00793 7 ALA 0.6941 0.00736 8 GLY 0.6953 0.00803 9 PHE 0.5489 0.00674 10 ILE 0.6608 0.00824 11 GLU 0.6503 0.00733 12 GLY 0.6196 0.00793 13 GLY 0.6752 0.00777 14 TRP 0.6894 0.00882 15 THR 0.6616 0.00638 16 GLY 0.7156 0.00749 17 MET 0.6717 0.00817 18 ILE 0.6633 0.00823 19 ASP 0.7017 0.00676 20 GLY 0.6845 0.00766 21 TRP 0.6481 0.00726 22 TYR 0.6646 0.00804 23 GLY 0.4875 0.00642 stop_ save_ save_H_exch_rate_pH7 _Saveframe_category H_exchange_rates _Details . loop_ _Sample_label $pH_7.4_sample stop_ _Sample_conditions_label $pH_7.4_Sample_condition _H_exchange_rate_units s-1 _Mol_system_component_name HAfp23-F3G _Text_data_format . _Text_data . loop_ _Residue_seq_code _Residue_label _Atom_name _H_exchange_rate_value _H_exchange_rate_min_value _H_exchange_rate_max_value _H_exchange_rate_value_error 3 GLY H 8.53934 . . . 4 GLY H 7.19348 . . . 5 ALA H 4.16480 . . . 6 ILE H 1.06743 . . . 7 ALA H 1.21041 . . . 8 GLY H 2.89972 . . . 9 PHE H 8.90517 . . . 10 ILE H 1.32725 . . . 11 GLU H 1.62253 . . . 12 GLY H 4.65513 . . . 13 GLY H 5.69954 . . . 14 TRP H 9.61916 . . . 15 THR H 5.80902 . . . 16 GLY H 3.76586 . . . 17 MET H 5.01204 . . . 18 ILE H 1.49679 . . . 19 ASP H 2.75504 . . . 20 GLY H 3.39632 . . . 21 TRP H 3.81525 . . . 22 TYR H 3.18645 . . . 23 GLY H 7.90474 . . . stop_ save_