data_19696 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Nuclear magnetic resonance studies of N2-guanine adducts derived from the tumorigen dibenzo[a,l]pyrene in DNA: Impact of adduct stereochemistry, size, and local DNA structure on solution conformations ; _BMRB_accession_number 19696 _BMRB_flat_file_name bmr19696.str _Entry_type original _Submission_date 2013-12-20 _Accession_date 2013-12-20 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 'Rodr guez' 'Fabi n' A . 2 Liu Zhi . . 3 Lin Chin H. . 4 Ding Shuang . . 5 Cai Yuqin . . 6 Kolbanovskiy Alexander . . 7 Kolbanovskiy Marina . . 8 Amin Shantu . . 9 Broyde Suse . . 10 Geacintov Nicholas E. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 3 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 154 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2014-03-31 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 19695 'N2-guanine adducts derived from the tumorigen dibenzo[a,l]pyrene in DNA (1)' stop_ _Original_release_date 2014-03-31 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title ; Nuclear Magnetic Resonance Studies of an N(2)-Guanine Adduct Derived from the Tumorigen Dibenzo[a,l]pyrene in DNA: Impact of Adduct Stereochemistry, Size, and Local DNA Sequence on Solution Conformations ; _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 24617538 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Rodriguez Fabian A. . 2 Liu Zhi . . 3 Lin Chin H. . 4 Ding Shuang . . 5 Cai Yuqin . . 6 Kolbanovskiy Alexander . . 7 Kolbanovskiy Marina . . 8 Amin Shantu . . 9 Broyde Suse . . 10 Geacintov Nicholas E. . stop_ _Journal_abbreviation Biochemistry _Journal_volume 53 _Journal_issue 11 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1827 _Page_last 1841 _Year 2014 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'N2-guanine adducts derived from the tumorigen dibenzo[a,l]pyrene in DNA' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'DNA (5'-D(*CP*CP*AP*TP*CP*GP*CP*TP*AP*CP*C)-3')' $DNA_(5'-D(*CP*CP*AP*TP*CP*GP*CP*TP*AP*CP*C)-3') 'DNA (5'-D(*GP*GP*TP*AP*GP*CP*GP*AP*TP*GP*G)-3')' $DNA_(5'-D(*GP*GP*TP*AP*GP*CP*GP*AP*TP*GP*G)-3') (11R,12R,13R)-11,12,13,14-tetrahydronaphtho[1,2,3,4-pqr]tetraphene-11,12,13-triol $entity_2QL stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_DNA_(5'-D(*CP*CP*AP*TP*CP*GP*CP*TP*AP*CP*C)-3') _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class DNA _Name_common DNA_(5'-D(*CP*CP*AP*TP*CP*GP*CP*TP*AP*CP*C)-3') _Molecular_mass 3254.165 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 11 _Mol_residue_sequence CCATCGCTACC loop_ _Residue_seq_code _Residue_label 1 DC 2 DC 3 DA 4 DT 5 DC 6 DG 7 DC 8 DT 9 DA 10 DC 11 DC stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ save_DNA_(5'-D(*GP*GP*TP*AP*GP*CP*GP*AP*TP*GP*G)-3') _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class DNA _Name_common DNA_(5'-D(*GP*GP*TP*AP*GP*CP*GP*AP*TP*GP*G)-3') _Molecular_mass 3454.286 _Mol_thiol_state 'not present' _Details . _Residue_count 11 _Mol_residue_sequence GGTAGCGATGG loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 12 DG 2 13 DG 3 14 DT 4 15 DA 5 16 DG 6 17 DC 7 18 DG 8 19 DA 9 20 DT 10 21 DG 11 22 DG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ ############# # Ligands # ############# save_2QL _Saveframe_category ligand _Mol_type "non-polymer (NON-POLYMER)" _Name_common (11R,12R,13R)-11,12,13,14-tetrahydronaphtho[1,2,3,4-pqr]tetraphene-11,12,13-triol _BMRB_code 2QL _PDB_code 2QL _Molecular_mass 354.398 _Mol_charge 0 _Mol_paramagnetic . _Mol_aromatic yes _Details . loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons C14 C14 C . 0 . ? C13 C13 C . 0 . ? O13 O13 O . 0 . ? C12 C12 C . 0 . ? O12 O12 O . 0 . ? C11 C11 C . 0 . ? O11 O11 O . 0 . ? C16 C16 C . 0 . ? C10 C10 C . 0 . ? C17 C17 C . 0 . ? C18 C18 C . 0 . ? C9 C9 C . 0 . ? C8 C8 C . 0 . ? C20 C20 C . 0 . ? C21 C21 C . 0 . ? C7 C7 C . 0 . ? C6 C6 C . 0 . ? C5 C5 C . 0 . ? C22 C22 C . 0 . ? C23 C23 C . 0 . ? C4 C4 C . 0 . ? C3 C3 C . 0 . ? C2 C2 C . 0 . ? C1 C1 C . 0 . ? C24 C24 C . 0 . ? C19 C19 C . 0 . ? C15 C15 C . 0 . ? H14 H14 H . 0 . ? H15 H15 H . 0 . ? H13 H13 H . 0 . ? HO13 HO13 H . 0 . ? H12 H12 H . 0 . ? HO12 HO12 H . 0 . ? H11 H11 H . 0 . ? HO11 HO11 H . 0 . ? H10 H10 H . 0 . ? H9 H9 H . 0 . ? H8 H8 H . 0 . ? H7 H7 H . 0 . ? H6 H6 H . 0 . ? H5 H5 H . 0 . ? H4 H4 H . 0 . ? H3 H3 H . 0 . ? H2 H2 H . 0 . ? H1 H1 H . 0 . ? stop_ loop_ _Bond_order _Bond_atom_one_atom_name _Bond_atom_two_atom_name _PDB_bond_atom_one_atom_name _PDB_bond_atom_two_atom_name DOUB C7 C6 ? ? SING C7 C20 ? ? SING C6 C5 ? ? DOUB C8 C20 ? ? SING C8 C9 ? ? SING C20 C21 ? ? DOUB C5 C22 ? ? DOUB C9 C17 ? ? SING C21 C22 ? ? DOUB C21 C18 ? ? SING C22 C23 ? ? SING C17 C18 ? ? SING C17 C10 ? ? SING C18 C19 ? ? DOUB C23 C4 ? ? SING C23 C24 ? ? SING C4 C3 ? ? DOUB C10 C16 ? ? SING C19 C24 ? ? DOUB C19 C15 ? ? DOUB C24 C1 ? ? DOUB C3 C2 ? ? SING C16 C15 ? ? SING C16 C11 ? ? SING C15 C14 ? ? SING C1 C2 ? ? SING C11 O11 ? ? SING C11 C12 ? ? SING O13 C13 ? ? SING C14 C13 ? ? SING C13 C12 ? ? SING C12 O12 ? ? SING C14 H14 ? ? SING C14 H15 ? ? SING C13 H13 ? ? SING O13 HO13 ? ? SING C12 H12 ? ? SING O12 HO12 ? ? SING C11 H11 ? ? SING O11 HO11 ? ? SING C10 H10 ? ? SING C9 H9 ? ? SING C8 H8 ? ? SING C7 H7 ? ? SING C6 H6 ? ? SING C5 H5 ? ? SING C4 H4 ? ? SING C3 H3 ? ? SING C2 H2 ? ? SING C1 H1 ? ? stop_ _Mol_thiol_state . _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $DNA_(5'-D(*CP*CP*AP*TP*CP*GP*CP*TP*AP*CP*C)-3') . . . . . . $DNA_(5'-D(*GP*GP*TP*AP*GP*CP*GP*AP*TP*GP*G)-3') . . . . . . stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $DNA_(5'-D(*CP*CP*AP*TP*CP*GP*CP*TP*AP*CP*C)-3') 'chemical synthesis' . . . . . $DNA_(5'-D(*GP*GP*TP*AP*GP*CP*GP*AP*TP*GP*G)-3') 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $DNA_(5'-D(*CP*CP*AP*TP*CP*GP*CP*TP*AP*CP*C)-3') 1.5 mM 'natural abundance' NaCl 100 mM 'natural abundance' Na2HPO4 10 mM 'natural abundance' DSS 25 mM 'natural abundance' D20 100 % 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $DNA_(5'-D(*CP*CP*AP*TP*CP*GP*CP*TP*AP*CP*C)-3') 1.5 mM 'natural abundance' NaCl 100 mM 'natural abundance' Na2HPO4 10 mM 'natural abundance' DSS 25 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection processing stop_ _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' stop_ _Details . save_ save_AMBER _Saveframe_category software _Name AMBER _Version . loop_ _Vendor _Address _Electronic_address 'Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman' . . stop_ loop_ _Task refinement stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_TOCSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.8 . pH stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA (5'-D(*CP*CP*AP*TP*CP*GP*CP*TP*AP*CP*C)-3')' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 DC H1' H 5.913 . . 2 1 1 DC H2' H 2.487 . . 3 1 1 DC H2'' H 2.094 . . 4 1 1 DC H3' H 4.637 . . 5 1 1 DC H4' H 4.090 . . 6 1 1 DC H5 H 5.884 . . 7 1 1 DC H6 H 7.736 . . 8 2 2 DC H1' H 5.382 . . 9 2 2 DC H2' H 2.414 . . 10 2 2 DC H2'' H 2.175 . . 11 2 2 DC H3' H 4.834 . . 12 2 2 DC H4' H 4.108 . . 13 2 2 DC H5 H 5.668 . . 14 2 2 DC H6 H 7.598 . . 15 3 3 DA H1' H 6.230 . . 16 3 3 DA H2 H 7.697 . . 17 3 3 DA H2' H 2.875 . . 18 3 3 DA H2'' H 2.692 . . 19 3 3 DA H3' H 5.007 . . 20 3 3 DA H4' H 4.413 . . 21 3 3 DA H8 H 8.326 . . 22 4 4 DT H1' H 5.770 . . 23 4 4 DT H2' H 2.238 . . 24 4 4 DT H2'' H 1.822 . . 25 4 4 DT H3' H 4.769 . . 26 4 4 DT H4' H 4.081 . . 27 4 4 DT H6 H 7.024 . . 28 4 4 DT H71 H 1.261 . . 29 4 4 DT H72 H 1.261 . . 30 4 4 DT H73 H 1.261 . . 31 5 5 DC H1' H 5.777 . . 32 5 5 DC H2' H 2.452 . . 33 5 5 DC H2'' H 2.326 . . 34 5 5 DC H5 H 5.336 . . 35 5 5 DC H6 H 7.312 . . 36 6 6 DG H1' H 6.099 . . 37 6 6 DG H2' H 2.865 . . 38 6 6 DG H2'' H 2.662 . . 39 6 6 DG H4' H 4.486 . . 40 6 6 DG H8 H 8.027 . . 41 7 7 DC H1' H 5.744 . . 42 7 7 DC H2' H 2.439 . . 43 7 7 DC H2'' H 1.953 . . 44 7 7 DC H3' H 4.626 . . 45 7 7 DC H4' H 4.209 . . 46 7 7 DC H5 H 5.353 . . 47 7 7 DC H6 H 7.356 . . 48 8 8 DT H1' H 5.599 . . 49 8 8 DT H2' H 2.437 . . 50 8 8 DT H2'' H 2.117 . . 51 8 8 DT H6 H 7.372 . . 52 8 8 DT H71 H 1.601 . . 53 8 8 DT H72 H 1.601 . . 54 8 8 DT H73 H 1.601 . . 55 9 9 DA H1' H 6.175 . . 56 9 9 DA H2 H 7.470 . . 57 9 9 DA H2' H 2.848 . . 58 9 9 DA H2'' H 2.720 . . 59 9 9 DA H3' H 5.011 . . 60 9 9 DA H4' H 4.407 . . 61 9 9 DA H8 H 8.305 . . 62 10 10 DC H1' H 5.870 . . 63 10 10 DC H2' H 2.379 . . 64 10 10 DC H2'' H 2.022 . . 65 10 10 DC H3' H 4.727 . . 66 10 10 DC H4' H 4.136 . . 67 10 10 DC H5 H 5.342 . . 68 10 10 DC H6 H 7.324 . . 69 11 11 DC H1' H 6.152 . . 70 11 11 DC H2' H 2.217 . . 71 11 11 DC H2'' H 2.217 . . 72 11 11 DC H3' H 4.511 . . 73 11 11 DC H4' H 3.983 . . 74 11 11 DC H5 H 5.604 . . 75 11 11 DC H6 H 7.548 . . stop_ save_ save_assigned_chem_shift_list_1_2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA (5'-D(*GP*GP*TP*AP*GP*CP*GP*AP*TP*GP*G)-3')' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 12 1 DG H1' H 5.697 . . 2 12 1 DG H2' H 2.610 . . 3 12 1 DG H2'' H 2.704 . . 4 12 1 DG H3' H 4.803 . . 5 12 1 DG H8 H 7.883 . . 6 13 2 DG H1' H 5.996 . . 7 13 2 DG H2' H 2.628 . . 8 13 2 DG H2'' H 2.780 . . 9 13 2 DG H3' H 4.965 . . 10 13 2 DG H4' H 4.396 . . 11 13 2 DG H5' H 4.177 . . 12 13 2 DG H5'' H 4.105 . . 13 13 2 DG H8 H 7.817 . . 14 14 3 DT H1' H 5.568 . . 15 14 3 DT H2' H 2.096 . . 16 14 3 DT H2'' H 2.384 . . 17 14 3 DT H3' H 4.846 . . 18 14 3 DT H4' H 4.171 . . 19 14 3 DT H6 H 7.279 . . 20 14 3 DT H71 H 1.420 . . 21 14 3 DT H72 H 1.420 . . 22 14 3 DT H73 H 1.420 . . 23 15 4 DA H1' H 5.922 . . 24 15 4 DA H2 H 7.343 . . 25 15 4 DA H2' H 2.619 . . 26 15 4 DA H2'' H 2.750 . . 27 15 4 DA H3' H 4.991 . . 28 15 4 DA H4' H 4.352 . . 29 15 4 DA H5' H 4.094 . . 30 15 4 DA H5'' H 3.993 . . 31 15 4 DA H8 H 8.122 . . 32 16 5 DG H1' H 5.287 . . 33 16 5 DG H2' H 2.222 . . 34 16 5 DG H2'' H 2.148 . . 35 16 5 DG H3' H 4.811 . . 36 16 5 DG H4' H 4.203 . . 37 16 5 DG H8 H 7.467 . . 38 17 6 DC H2' H 1.992 . . 39 17 6 DC H2'' H 2.348 . . 40 17 6 DC H5 H 4.462 . . 41 17 6 DC H6 H 6.786 . . 42 19 8 DA H1' H 5.884 . . 43 19 8 DA H2' H 2.495 . . 44 19 8 DA H2'' H 2.757 . . 45 19 8 DA H8 H 8.035 . . 46 20 9 DT H1' H 5.564 . . 47 20 9 DT H2' H 1.745 . . 48 20 9 DT H2'' H 2.154 . . 49 20 9 DT H3' H 4.764 . . 50 20 9 DT H4' H 4.085 . . 51 20 9 DT H6 H 7.020 . . 52 20 9 DT H71 H 1.296 . . 53 20 9 DT H72 H 1.296 . . 54 20 9 DT H73 H 1.296 . . 55 21 10 DG H1' H 5.603 . . 56 21 10 DG H2' H 2.605 . . 57 21 10 DG H2'' H 2.674 . . 58 21 10 DG H3' H 4.314 . . 59 21 10 DG H8 H 7.767 . . 60 22 11 DG H1' H 6.096 . . 61 22 11 DG H2' H 2.483 . . 62 22 11 DG H2'' H 2.319 . . 63 22 11 DG H3' H 4.624 . . 64 22 11 DG H4' H 4.185 . . 65 22 11 DG H8 H 7.756 . . stop_ save_ save_assigned_chem_shift_list_1_3 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name (11R,12R,13R)-11,12,13,14-tetrahydronaphtho[1,2,3,4-pqr]tetraphene-11,12,13-triol _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 101 1 2QL H1 H 8.105 . . 2 101 1 2QL H10 H 7.598 . . 3 101 1 2QL H11 H 4.038 . . 4 101 1 2QL H12 H 5.098 . . 5 101 1 2QL H13 H 4.273 . . 6 101 1 2QL H14 H 6.484 . . 7 101 1 2QL H2 H 6.705 . . 8 101 1 2QL H3 H 6.744 . . 9 101 1 2QL H4 H 8.107 . . 10 101 1 2QL H5 H 8.357 . . 11 101 1 2QL H6 H 7.668 . . 12 101 1 2QL H7 H 7.794 . . 13 101 1 2QL H8 H 7.813 . . 14 101 1 2QL H9 H 8.371 . . stop_ save_