data_19399 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; NMR structure of EKLF(22-40)/Ubiquitin Complex ; _BMRB_accession_number 19399 _BMRB_flat_file_name bmr19399.str _Entry_type original _Submission_date 2013-07-31 _Accession_date 2013-07-31 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Raiola Luca . . 2 Omichinski James G. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 2 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 583 "13C chemical shifts" 339 "15N chemical shifts" 101 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2013-12-06 update BMRB 'update entry citation' 2013-10-08 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Structural Characterization of a Noncovalent Complex between Ubiquitin and the Transactivation Domain of the Erythroid-Specific Factor EKLF.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 24139988 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Raiola Luca . . 2 Lussier-Price Mathieu . . 3 Gagnon David . . 4 Lafrance-Vanasse Julien . . 5 Mascle Xavier . . 6 Arseneault Genevieve . . 7 Legault Pascale . . 8 Archambault Jacques . . 9 Omichinski James G. . stop_ _Journal_abbreviation Structure _Journal_name_full 'Structure (London, England : 1993)' _Journal_volume 21 _Journal_issue 11 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 2014 _Page_last 2024 _Year 2013 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'EKLF(22-40)/Ubiquitin Complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label EKLF $EKLF Ubiquitin $Ubiquitin stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_EKLF _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common EKLF _Molecular_mass 2359.502 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 19 _Mol_residue_sequence DTQDDFLKWWRSEEAQDMG loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 22 ASP 2 23 THR 3 24 GLN 4 25 ASP 5 26 ASP 6 27 PHE 7 28 LEU 8 29 LYS 9 30 TRP 10 31 TRP 11 32 ARG 12 33 SER 13 34 GLU 14 35 GLU 15 36 ALA 16 37 GLN 17 38 ASP 18 39 MET 19 40 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-11-04 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2MBH "Nmr Structure Of Eklf(22-40)/ubiquitin Complex" 100.00 41 100.00 100.00 3.12e-04 DBJ BAG73333 "Kruppel-like factor 1 [synthetic construct]" 100.00 362 100.00 100.00 3.94e-05 GB AAB51173 "erythroid Kruppel-like factor [Homo sapiens]" 100.00 362 100.00 100.00 3.94e-05 GB AAC50562 "erythroid Kruppel-like factor EKLF [Homo sapiens]" 100.00 362 100.00 100.00 3.94e-05 GB AAC51108 "erythroid-specific transcription factor EKLF [Homo sapiens]" 100.00 362 100.00 100.00 4.31e-05 GB AAH33580 "Kruppel-like factor 1 (erythroid) [Homo sapiens]" 100.00 362 100.00 100.00 3.50e-05 GB AFW90650 "krueppel-like factor 1 [Homo sapiens]" 100.00 353 100.00 100.00 3.06e-05 REF NP_001181384 "Krueppel-like factor 1 [Macaca mulatta]" 100.00 362 100.00 100.00 3.02e-05 REF NP_006554 "Krueppel-like factor 1 [Homo sapiens]" 100.00 362 100.00 100.00 3.94e-05 REF XP_002761831 "PREDICTED: Krueppel-like factor 1 [Callithrix jacchus]" 100.00 362 100.00 100.00 5.04e-05 REF XP_002828790 "PREDICTED: Krueppel-like factor 1 [Pongo abelii]" 100.00 362 100.00 100.00 3.40e-05 REF XP_003275639 "PREDICTED: Krueppel-like factor 1 [Nomascus leucogenys]" 100.00 362 100.00 100.00 4.06e-05 SP Q13351 "RecName: Full=Krueppel-like factor 1; AltName: Full=Erythroid krueppel-like transcription factor; Short=EKLF" 100.00 362 100.00 100.00 3.94e-05 stop_ save_ save_Ubiquitin _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common Ubiquitin _Molecular_mass 8576.914 _Mol_thiol_state 'not present' _Details . _Residue_count 76 _Mol_residue_sequence ; MQIFVKTLTGKTITLEVEPS DTIENVKAKIQDKEGIPPDQ QRLIFAGKQLEDGRTLSDYN IQKESTLHLVLRLRGG ; loop_ _Residue_seq_code _Residue_label 1 MET 2 GLN 3 ILE 4 PHE 5 VAL 6 LYS 7 THR 8 LEU 9 THR 10 GLY 11 LYS 12 THR 13 ILE 14 THR 15 LEU 16 GLU 17 VAL 18 GLU 19 PRO 20 SER 21 ASP 22 THR 23 ILE 24 GLU 25 ASN 26 VAL 27 LYS 28 ALA 29 LYS 30 ILE 31 GLN 32 ASP 33 LYS 34 GLU 35 GLY 36 ILE 37 PRO 38 PRO 39 ASP 40 GLN 41 GLN 42 ARG 43 LEU 44 ILE 45 PHE 46 ALA 47 GLY 48 LYS 49 GLN 50 LEU 51 GLU 52 ASP 53 GLY 54 ARG 55 THR 56 LEU 57 SER 58 ASP 59 TYR 60 ASN 61 ILE 62 GLN 63 LYS 64 GLU 65 SER 66 THR 67 LEU 68 HIS 69 LEU 70 VAL 71 LEU 72 ARG 73 LEU 74 ARG 75 GLY 76 GLY stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-29 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 11505 entity 100.00 76 98.68 98.68 2.41e-45 BMRB 11547 ubiquitin 100.00 76 98.68 98.68 2.41e-45 BMRB 15047 denatured_ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 15410 Ubi 100.00 76 100.00 100.00 4.45e-46 BMRB 15689 UBB 98.68 103 98.67 100.00 4.23e-44 BMRB 15866 ubiquitin 98.68 76 100.00 100.00 2.71e-45 BMRB 15907 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 16228 ubiquitin 100.00 76 97.37 98.68 1.18e-44 BMRB 16582 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 16626 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 16763 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 16880 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 16885 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 16895 UBB+1 98.68 103 98.67 100.00 4.23e-44 BMRB 17059 ubiquitin 100.00 156 100.00 100.00 5.21e-46 BMRB 17181 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 17239 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 17333 UB 100.00 76 100.00 100.00 4.45e-46 BMRB 17439 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 17769 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 17919 entity 100.00 76 100.00 100.00 4.45e-46 BMRB 18582 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 18583 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 18584 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 18610 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 18611 Ubiquitin_A_state 100.00 76 100.00 100.00 4.45e-46 BMRB 18737 UBIQUITIN 100.00 76 100.00 100.00 4.45e-46 BMRB 19394 ubiquitin 100.00 79 100.00 100.00 4.53e-46 BMRB 19406 entity 100.00 152 100.00 100.00 4.37e-45 BMRB 19412 entity 100.00 152 100.00 100.00 4.37e-45 BMRB 19447 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 25070 Ubiquitin 100.00 79 100.00 100.00 4.53e-46 BMRB 25230 Ubiquitin 100.00 78 100.00 100.00 3.66e-46 BMRB 4245 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 4375 Ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 4983 Ubiquitin 98.68 76 97.33 100.00 3.66e-44 BMRB 5101 uq1_51 67.11 53 100.00 100.00 2.13e-26 BMRB 5387 ubq 100.00 76 100.00 100.00 4.45e-46 BMRB 6457 Ub 100.00 76 100.00 100.00 4.45e-46 BMRB 6466 Ub 100.00 76 100.00 100.00 4.45e-46 BMRB 6470 Ub 100.00 76 100.00 100.00 4.45e-46 BMRB 6488 Ub 100.00 76 100.00 100.00 4.45e-46 BMRB 68 ubiquitin 100.00 76 100.00 100.00 4.45e-46 BMRB 7111 human_ubiquitin 100.00 76 100.00 100.00 4.45e-46 PDB 1AAR "Structure Of A Diubiquitin Conjugate And A Model For Interaction With Ubiquitin Conjugating Enzyme (E2)" 100.00 76 100.00 100.00 4.45e-46 PDB 1CMX "Structural Basis For The Specificity Of Ubiquitin C- Terminal Hydrolases" 98.68 76 100.00 100.00 2.99e-45 PDB 1D3Z "Ubiquitin Nmr Structure" 100.00 76 100.00 100.00 4.45e-46 PDB 1F9J "Structure Of A New Crystal Form Of Tetraubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 1FXT "Structure Of A Conjugating Enzyme-Ubiquitin Thiolester Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 1G6J "Structure Of Recombinant Human Ubiquitin In Aot Reverse Micelles" 98.68 76 100.00 100.00 5.16e-45 PDB 1GJZ "Solution Structure Of A Dimeric N-Terminal Fragment Of Human Ubiquitin" 67.11 53 100.00 100.00 2.13e-26 PDB 1NBF "Crystal Structure Of A Ubp-Family Deubiquitinating Enzyme In Isolation And In Complex With Ubiquitin Aldehyde" 98.68 76 100.00 100.00 2.99e-45 PDB 1OGW "Synthetic Ubiquitin With Fluoro-Leu At 50 And 67" 100.00 76 97.37 97.37 1.80e-44 PDB 1P3Q "Mechanism Of Ubiquitin Recognition By The Cue Domain Of Vps9" 100.00 76 100.00 100.00 4.45e-46 PDB 1Q5W "Ubiquitin Recognition By Npl4 Zinc-Fingers" 100.00 76 100.00 100.00 4.45e-46 PDB 1S1Q "Tsg101(Uev) Domain In Complex With Ubiquitin" 98.68 76 100.00 100.00 5.16e-45 PDB 1TBE "Structure Of Tetraubiquitin Shows How Multiubiquitin Chains Can Be Formed" 100.00 76 100.00 100.00 4.45e-46 PDB 1UBI "Synthetic Structural And Biological Studies Of The Ubiquitin System. Part 1" 100.00 76 100.00 100.00 4.45e-46 PDB 1UBQ "Structure Of Ubiquitin Refined At 1.8 Angstroms Resolution" 100.00 76 100.00 100.00 4.45e-46 PDB 1UZX "A Complex Of The Vps23 Uev With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 1V80 "Solution Structures Of Ubiquitin At 30 Bar And 3 Kbar" 100.00 76 100.00 100.00 4.45e-46 PDB 1V81 "Solution Structures Of Ubiquitin At 30 Bar And 3 Kbar" 100.00 76 100.00 100.00 4.45e-46 PDB 1VX7 "Cryo-em Structure Of The Plasmodium Falciparum 80s Ribosome Bound To The Anti-protozoan Drug Emetine, Large Subunit (protein On" 100.00 128 98.68 100.00 9.37e-46 PDB 1WR6 "Crystal Structure Of Gga3 Gat Domain In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 1WRD "Crystal Structure Of Tom1 Gat Domain In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 1XD3 "Crystal Structure Of Uchl3-Ubvme Complex" 98.68 75 100.00 100.00 2.91e-45 PDB 1XQQ "Simultaneous Determination Of Protein Structure And Dynamics" 100.00 76 100.00 100.00 4.45e-46 PDB 1YD8 "Complex Of Human Gga3 Gat Domain And Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 1YIW "X-Ray Crystal Structure Of A Chemically Synthesized Ubiquitin" 100.00 76 98.68 100.00 1.94e-45 PDB 1YJ1 "X-Ray Crystal Structure Of A Chemically Synthesized [d-Gln35]ubiquitin" 100.00 76 97.37 98.68 2.29e-44 PDB 1YX5 "Solution Structure Of S5a Uim-1UBIQUITIN COMPLEX" 100.00 98 100.00 100.00 1.57e-46 PDB 1YX6 "Solution Structure Of S5a Uim-2UBIQUITIN COMPLEX" 100.00 98 100.00 100.00 1.57e-46 PDB 1ZGU "Solution Structure Of The Human Mms2-Ubiquitin Complex" 100.00 76 98.68 100.00 1.06e-45 PDB 2AYO "Structure Of Usp14 Bound To Ubquitin Aldehyde" 98.68 76 100.00 100.00 2.99e-45 PDB 2BGF "Nmr Structure Of Lys48-Linked Di-Ubiquitin Using Chemical Shift Perturbation Data Together With Rdcs And 15n- Relaxation Data" 100.00 76 100.00 100.00 4.45e-46 PDB 2C7M "Human Rabex-5 Residues 1-74 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2C7N "Human Rabex-5 Residues 1-74 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2D3G "Double Sided Ubiquitin Binding Of Hrs-Uim" 100.00 76 100.00 100.00 4.45e-46 PDB 2DEN "Solution Structure Of The Ubiquitin-Associated Domain Of Human Bmsc-Ubp And Its Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2DX5 "The Complex Structure Between The Mouse Eap45-Glue Domain And Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2FCM "X-Ray Crystal Structure Of A Chemically Synthesized [d-Gln35]ubiquitin With A Cubic Space Group" 100.00 76 97.37 98.68 2.29e-44 PDB 2FCN "X-Ray Crystal Structure Of A Chemically Synthesized [d-Val35]ubiquitin With A Cubic Space Group" 100.00 76 97.37 98.68 2.29e-44 PDB 2FCQ "X-Ray Crystal Structure Of A Chemically Synthesized Ubiquitin With A Cubic Space Group" 100.00 76 98.68 100.00 1.94e-45 PDB 2FCS "X-Ray Crystal Structure Of A Chemically Synthesized [l-Gln35]ubiquitin With A Cubic Space Group" 100.00 76 97.37 98.68 2.61e-44 PDB 2FID "Crystal Structure Of A Bovine Rabex-5 Fragment Complexed With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2FIF "Crystal Structure Of A Bovine Rabex-5 Fragment Complexed With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2FUH "Solution Structure Of The Ubch5cUB NON-Covalent Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 2G45 "Co-Crystal Structure Of Znf Ubp Domain From The Deubiquitinating Enzyme Isopeptidase T (Isot) In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2GMI Mms2UBC13~UBIQUITIN 100.00 76 100.00 100.00 4.45e-46 PDB 2HD5 "Usp2 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2HTH "Structural Basis For Ubiquitin Recognition By The Human Eap45ESCRT-Ii Glue Domain" 100.00 76 100.00 100.00 4.45e-46 PDB 2IBI "Covalent Ubiquitin-Usp2 Complex" 98.68 75 100.00 100.00 2.91e-45 PDB 2J7Q "Crystal Structure Of The Ubiquitin-Specific Protease Encoded By Murine Cytomegalovirus Tegument Protein M48 In Complex With A U" 98.68 75 100.00 100.00 2.91e-45 PDB 2JF5 "Crystal Structure Of Lys63-Linked Di-Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2JRI "Solution Structure Of The Josephin Domain Of Ataxin-3 In Complex With Ubiquitin Molecule." 100.00 76 100.00 100.00 4.45e-46 PDB 2JY6 "Solution Structure Of The Complex Of Ubiquitin And Ubiquilin 1 Uba Domain" 100.00 76 100.00 100.00 4.45e-46 PDB 2JZZ "Solid-State Nmr Structure Of Microcrystalline Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2K25 "Automated Nmr Structure Of The Ubb By Fapsy" 98.68 103 98.67 100.00 4.23e-44 PDB 2K39 "Recognition Dynamics Up To Microseconds Revealed From Rdc Derived Ubiquitin Ensemble In Solution" 100.00 76 100.00 100.00 4.45e-46 PDB 2K6D "Cin85 Sh3-C Domain In Complex With Ubiquitin" 98.68 76 100.00 100.00 2.71e-45 PDB 2K8B "Solution Structure Of Plaa Family Ubiquitin Binding Domain (Pfuc) Cis Isomer In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2K8C "Solution Structure Of Plaa Family Ubiquitin Binding Domain (Pfuc) Trans Isomer In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2KDE "Nmr Structure Of Major S5a (196-306):k48 Linked Diubiquitin Species" 100.00 76 100.00 100.00 4.45e-46 PDB 2KDF "Nmr Structure Of Minor S5a (196-306):k48 Linked Diubiquitin Species" 100.00 76 100.00 100.00 4.45e-46 PDB 2KHW "Solution Structure Of The Human Polymerase Iota Ubm2- Ubiquitin Complex" 100.00 79 100.00 100.00 4.53e-46 PDB 2KJH "Nmr Based Structural Model Of The Ubch8-Ubiquitin Complex" 98.68 76 100.00 100.00 2.71e-45 PDB 2KLG "Pere Nmr Structure Of Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2KN5 "A Correspondence Between Solution-State Dynamics Of An Individual Protein And The Sequence And Conformational Diversity Of Its " 100.00 76 100.00 100.00 4.45e-46 PDB 2KOX "Nmr Residual Dipolar Couplings Identify Long Range Correlated Motions In The Backbone Of The Protein Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2KTF "Solution Nmr Structure Of Human Polymerase Iota Ubm2 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2KWU "Solution Structure Of Ubm2 Of Murine Polymerase Iota In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2KWV "Solution Structure Of Ubm1 Of Murine Polymerase Iota In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2KX0 "The Solution Structure Of Ubb+1, Frameshift Mutant Of Ubiquitin B" 98.68 103 98.67 100.00 4.23e-44 PDB 2L0F "Solution Nmr Structure Of Human Polymerase Iota Ubm2 (P692a Mutant) In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2L0T "Solution Structure Of The Complex Of Ubiquitin And The Vhs Domain Of Stam2" 100.00 76 100.00 100.00 4.45e-46 PDB 2L3Z "Proton-Detected 4d Dream Solid-State Nmr Structure Of Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2LD9 "Backbone Structure Of Ubiquitin Determined Using Backbone Amide Noes And Backbone N-H And N-C Rdcs" 100.00 77 100.00 100.00 5.39e-46 PDB 2LJ5 "Description Of The Structural Fluctuations Of Proteins From Structure- Based Calculations Of Residual Dipolar Couplings" 100.00 76 100.00 100.00 4.45e-46 PDB 2LVO "Structure Of The Gp78cue Domain Bound To Monubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2LVP "Gp78cue Domain Bound To The Distal Ubiquitin Of K48-Linked Diubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2LVQ "Gp78cue Domain Bound To The Proximal Ubiquitin Of K48-Linked Diubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2LZ6 "Distinct Ubiquitin Binding Modes Exhibited By Sh3 Domains: Molecular Determinants And Functional Implications" 100.00 76 100.00 100.00 4.45e-46 PDB 2MBB "Solution Structure Of The Human Polymerase Iota Ubm1-ubiquitin Complex" 100.00 78 100.00 100.00 3.66e-46 PDB 2MBH "Nmr Structure Of Eklf(22-40)/ubiquitin Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 2MBO "K11-linked Diubiquitin Average Solution Structure At Ph 6.8, 0 Mm Nacl" 100.00 76 100.00 100.00 4.45e-46 PDB 2MBQ "K11-linked Diubiquitin Average Solution Structure At Ph 6.8, 150 Mm Nacl" 100.00 76 100.00 100.00 4.45e-46 PDB 2MCN "Distinct Ubiquitin Binding Modes Exhibited By Sh3 Domains: Molecular Determinants And Functional Implications" 100.00 76 100.00 100.00 4.45e-46 PDB 2MJ5 "Structure Of The Uba Domain Of Human Nbr1 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2MJB "Solution Nmr Structure Of Ubiquitin Refined Against Dipolar Couplings In 4 Media" 100.00 76 100.00 100.00 4.45e-46 PDB 2MOR "A Tensor-free Method For The Structural And Dynamical Refinement Of Proteins Using Residual Dipolar Couplings" 100.00 76 100.00 100.00 4.45e-46 PDB 2MRE "Nmr Structure Of The Rad18-ubz/ubiquitin Complex" 100.00 79 100.00 100.00 4.53e-46 PDB 2MUR "Solution Structure Of The Human Faap20 Ubz-ubiquitin Complex" 100.00 78 100.00 100.00 3.66e-46 PDB 2NR2 "The Mumo (Minimal Under-Restraining Minimal Over- Restraining) Method For The Determination Of Native States Ensembles Of Prote" 100.00 76 100.00 100.00 4.45e-46 PDB 2O6V "Crystal Structure And Solution Nmr Studies Of Lys48-Linked Tetraubiquitin At Neutral Ph" 100.00 76 100.00 100.00 4.45e-46 PDB 2OJR "Structure Of Ubiquitin Solved By Sad Using The Lanthanide- Binding Tag" 100.00 111 100.00 100.00 1.56e-45 PDB 2OOB "Crystal Structure Of The Uba Domain From Cbl-B Ubiquitin Ligase In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2PE9 "Nmr Based Structure Of The Open Conformation Of Lys48- Linked Di-Ubiquitin Using Experimental Global Rotational Diffusion Tenso" 100.00 76 100.00 100.00 4.45e-46 PDB 2PEA "Nmr Based Structure Of The Closed Conformation Of Lys48- Linked Di-Ubiquitin Using Experimental Global Rotational Diffusion Ten" 100.00 76 100.00 100.00 4.45e-46 PDB 2QHO "Crystal Structure Of The Uba Domain From Edd Ubiquitin Ligase In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2RR9 "The Solution Structure Of The K63-Ub2:tuims Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 2RSU "Alternative Structure Of Ubiquitin" 100.00 76 98.68 98.68 2.41e-45 PDB 2RU6 "The Pure Alternative State Of Ubiquitin" 100.00 76 98.68 98.68 2.41e-45 PDB 2W9N "Crystal Structure Of Linear Di-Ubiquitin" 98.68 152 100.00 100.00 3.82e-44 PDB 2WDT "Crystal Structure Of Plasmodium Falciparum Uchl3 In Complex With The Suicide Inhibitor Ubvme" 98.68 76 100.00 100.00 2.99e-45 PDB 2WWZ "Tab2 Nzf Domain In Complex With Lys63-Linked Di-Ubiquitin, P212121" 100.00 76 100.00 100.00 4.45e-46 PDB 2WX0 "Tab2 Nzf Domain In Complex With Lys63-Linked Di-Ubiquitin, P21" 100.00 76 100.00 100.00 4.45e-46 PDB 2WX1 "Tab2 Nzf Domain In Complex With Lys63-Linked Tri-Ubiquitin, P212121" 100.00 76 100.00 100.00 4.45e-46 PDB 2XBB "Nedd4 Hect:ub Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 2XEW "Crystal Structure Of K11-Linked Diubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2XK5 "Crystal Structure Of K6-Linked Diubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2Y5B "Structure Of Usp21 In Complex With Linear Diubiquitin-Aldehyde" 98.68 152 100.00 100.00 2.82e-44 PDB 2Z59 "Complex Structures Of Mouse Rpn13 (22-130aa) And Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 2ZCB "Crystal Structure Of Ubiquitin P37aP38A" 100.00 76 97.37 97.37 2.34e-44 PDB 2ZCC "Ubiquitin Crystallized Under High Pressure" 100.00 76 100.00 100.00 4.45e-46 PDB 2ZNV "Crystal Structure Of Human Amsh-Lp Dub Domain In Complex With Lys63-Linked Ubiquitin Dimer" 100.00 77 100.00 100.00 5.05e-46 PDB 2ZVN "Nemo Cozi Domain Incomplex With Diubiquitin In P212121 Space Group" 100.00 154 100.00 100.00 4.07e-45 PDB 2ZVO "Nemo Cozi Domain In Complex With Diubiquitin In C2 Space Group" 100.00 154 100.00 100.00 4.07e-45 PDB 3A1Q "Crystal Structure Of The Mouse Rap80 Uims In Complex With Lys63-Linked Di-Ubiquitin" 100.00 77 100.00 100.00 5.05e-46 PDB 3A33 "Ubch5b~ubiquitin Conjugate" 100.00 76 100.00 100.00 4.45e-46 PDB 3A9J "Crystal Structure Of The Mouse Tab2-Nzf In Complex With Lys63-Linked Di-Ubiquitin" 100.00 77 100.00 100.00 5.05e-46 PDB 3A9K "Crystal Structure Of The Mouse Tab3-Nzf In Complex With Lys63-Linked Di-Ubiquitin" 100.00 77 100.00 100.00 5.05e-46 PDB 3AI5 "Crystal Structure Of Yeast Enhanced Green Fluorescent Protein- Ubiquitin Fusion Protein" 97.37 307 100.00 100.00 1.12e-41 PDB 3ALB "Cyclic Lys48-Linked Tetraubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3AUL "Crystal Structure Of Wild-Type Lys48-Linked Diubiquitin In An Open Conformation" 100.00 76 100.00 100.00 4.45e-46 PDB 3AXC "Crystal Structure Of Linear Diubiquitin" 100.00 154 100.00 100.00 4.07e-45 PDB 3B08 "Crystal Structure Of The Mouse Hoil1-l-nzf In Complex With Linear Di- Ubiquitin" 100.00 152 100.00 100.00 4.37e-45 PDB 3B0A "Crystal Structure Of The Mouse Hoil1-l-nzf In Complex With Linear Di- Ubiquitin" 100.00 152 100.00 100.00 4.37e-45 PDB 3BY4 "Structure Of Ovarian Tumor (Otu) Domain In Complex With Ubiquitin" 98.68 75 100.00 100.00 2.91e-45 PDB 3C0R "Structure Of Ovarian Tumor (Otu) Domain In Complex With Ubiquitin" 98.68 75 100.00 100.00 2.91e-45 PDB 3DVG "Crystal Structure Of K63-Specific Fab Apu.3a8 Bound To K63-Linked Di- Ubiquitin" 100.00 80 100.00 100.00 5.61e-46 PDB 3DVN "Crystal Structure Of K63-specific Fab Apu2.16 Bound To K63-linked Di- Ubiquitin" 100.00 80 100.00 100.00 5.61e-46 PDB 3EEC "X-Ray Structure Of Human Ubiquitin Cd(Ii) Adduct" 100.00 76 100.00 100.00 4.45e-46 PDB 3EFU "X-Ray Structure Of Human Ubiquitin-Hg(Ii) Adduct" 100.00 76 100.00 100.00 4.45e-46 PDB 3EHV "X-Ray Structure Of Human Ubiquitin Zn(Ii) Adduct" 100.00 76 100.00 100.00 4.45e-46 PDB 3H1U "Structure Of Ubiquitin In Complex With Cd Ions" 100.00 76 100.00 100.00 4.45e-46 PDB 3H7P "Crystal Structure Of K63-Linked Di-Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3H7S "Crystal Structures Of K63-Linked Di- And Tri-Ubiquitin Reveal A Highly Extended Chain Architecture" 100.00 76 98.68 98.68 1.91e-43 PDB 3HM3 "The Structure And Conformation Of Lys-63 Linked Tetra-Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3I3T "Crystal Structure Of Covalent Ubiquitin-usp21 Complex" 98.68 75 100.00 100.00 2.91e-45 PDB 3IFW "Crystal Structure Of The S18y Variant Of Ubiquitin Carboxy T Hydrolase L1 Bound To Ubiquitin Vinylmethylester." 98.68 75 100.00 100.00 2.91e-45 PDB 3IHP "Covalent Ubiquitin-Usp5 Complex" 98.68 75 100.00 100.00 2.91e-45 PDB 3JSV "Crystal Structure Of Mouse Nemo Cozi In Complex With Lys63- Linked Di-Ubiquitin" 100.00 77 100.00 100.00 5.05e-46 PDB 3JVZ E2~ubiquitin-Hect 100.00 81 100.00 100.00 5.23e-46 PDB 3JW0 E2~ubiquitin-Hect 100.00 81 100.00 100.00 5.23e-46 PDB 3K9O "The Crystal Structure Of E2-25k And Ubb+1 Complex" 98.68 96 100.00 100.00 8.68e-45 PDB 3K9P "The Crystal Structure Of E2-25k And Ubiquitin Complex" 100.00 79 100.00 100.00 4.53e-46 PDB 3KVF "Crystal Structure Of The I93m Mutant Of Ubiquitin Carboxy Te Hydrolase L1 Bound To Ubiquitin Vinylmethylester" 98.68 75 100.00 100.00 2.91e-45 PDB 3KW5 "Crystal Structure Of Ubiquitin Carboxy Terminal Hydrolase L1 Ubiquitin Vinylmethylester" 98.68 75 100.00 100.00 2.91e-45 PDB 3LDZ "Crystal Structure Of Human Stam1 Vhs Domain In Complex With Ubiquitin" 96.05 73 100.00 100.00 1.22e-43 PDB 3M3J "A New Crystal Form Of Lys48-Linked Diubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3MHS "Structure Of The Saga Ubp8SGF11SUS1SGF73 DUB MODULE BOUND Ubiquitin Aldehyde" 98.68 76 100.00 100.00 2.99e-45 PDB 3MTN "Usp21 In Complex With A Ubiquitin-based, Usp21-specific Inhibitor" 88.16 85 98.51 98.51 1.00e-37 PDB 3N30 "Crystal Structure Of Cubic Zn3-Hub (Human Ubiquitin) Adduct" 100.00 76 100.00 100.00 4.45e-46 PDB 3N32 "The Crystal Structure Of Human Ubiquitin Adduct With Zeise's Salt" 100.00 76 100.00 100.00 4.45e-46 PDB 3NHE "High Resolution Structure (1.26a) Of Usp2a In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3NOB "Structure Of K11-linked Di-ubiquitin" 100.00 78 100.00 100.00 3.66e-46 PDB 3NS8 "Crystal Structure Of An Open Conformation Of Lys48-Linked Diubiquitin At Ph 7.5" 100.00 76 100.00 100.00 4.45e-46 PDB 3O65 "Crystal Structure Of A Josephin-Ubiquitin Complex: Evolutionary Restraints On Ataxin-3 Deubiquitinating Activity" 98.68 76 100.00 100.00 2.99e-45 PDB 3OFI "Crystal Structure Of Human Insulin-Degrading Enzyme In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3OJ3 "Crystal Structure Of The A20 Znf4 And Ubiquitin Complex" 100.00 79 100.00 100.00 4.53e-46 PDB 3OJ4 "Crystal Structure Of The A20 Znf4, Ubiquitin And Ubch5a Complex" 100.00 79 100.00 100.00 4.53e-46 PDB 3ONS "Crystal Structure Of Human Ubiquitin In A New Crystal Form" 94.74 72 100.00 100.00 5.80e-43 PDB 3PHD "Crystal Structure Of Human Hdac6 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3PHW "Otu Domain Of Crimean Congo Hemorrhagic Fever Virus In Complex With Ubiquitin" 98.68 75 100.00 100.00 2.91e-45 PDB 3PRM "Structural Analysis Of A Viral Otu Domain Protease From The Crimean- Congo Hemorrhagic Fever Virus In Complex With Human Ubiqui" 98.68 75 100.00 100.00 2.91e-45 PDB 3PRP "Structural Analysis Of A Viral Otu Domain Protease From The Crimean- Congo Hemorrhagic Fever Virus In Complex With Human Ubiqui" 98.68 75 100.00 100.00 2.91e-45 PDB 3PT2 "Structure Of A Viral Otu Domain Protease Bound To Ubiquitin" 98.68 75 100.00 100.00 2.91e-45 PDB 3PTF "X-Ray Structure Of The Non-Covalent Complex Between Ubch5a And Ubiquitin" 100.00 79 100.00 100.00 4.53e-46 PDB 3Q3F "Engineering Domain-Swapped Binding Interfaces By Mutually Exclusive Folding: Insertion Of Ubiquitin Into Position 103 Of Barnas" 98.68 189 100.00 100.00 3.64e-44 PDB 3RUL "New Strategy To Analyze Structures Of Glycopeptide-Target Complexes" 98.68 79 100.00 100.00 2.70e-45 PDB 3TBL "Structure Of Mono-ubiquitinated Pcna: Implications For Dna Polymerase Switching And Okazaki Fragment Maturation" 100.00 76 100.00 100.00 4.45e-46 PDB 3TMP "The Catalytic Domain Of Human Deubiquitinase Duba In Complex With Ubiquitin Aldehyde" 98.68 76 100.00 100.00 2.99e-45 PDB 3U30 "Crystal Structure Of A Linear-Specific Ubiquitin Fab Bound To Linear Ubiquitin" 100.00 172 100.00 100.00 4.69e-45 PDB 3UGB "Ubch5c~ubiquitin Conjugate" 100.00 76 100.00 100.00 4.45e-46 PDB 3VDZ "Tailoring Encodable Lanthanide-Binding Tags As Mri Contrast Agents: Xq-Dse3-Ubiquitin At 2.4 Angstroms" 100.00 111 100.00 100.00 9.99e-46 PDB 3VFK "The Structure Of Monodechloro-teicoplanin In Complex With Its Ligand, Using Ubiquitin As A Ligand Carrier" 98.68 79 100.00 100.00 2.70e-45 PDB 3VHT "Crystal Structure Of Gfp-Wrnip1 Ubz Domain Fusion Protein In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3VUW "Crystal Structure Of A20 Zf7 In Complex With Linear Ubiquitin, Form I" 100.00 76 100.00 100.00 4.45e-46 PDB 3VUX "Crystal Structure Of A20 Zf7 In Complex With Linear Ubiquitin, Form Ii" 100.00 76 100.00 100.00 4.45e-46 PDB 3VUY "Crystal Structure Of A20 Zf7 In Complex With Linear Tetraubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 3ZLZ "Lys6-linked Tri-ubiquitin" 100.00 76 98.68 100.00 1.06e-45 PDB 3ZNH "Crimean Congo Hemorrhagic Fever Virus Otu Domain In Complex With Ubiquitin-propargyl." 98.68 76 100.00 100.00 2.99e-45 PDB 3ZNI "Structure Of Phosphotyr363-cbl-b - Ubch5b-ub - Zap-70 Peptide Complex" 100.00 81 100.00 100.00 5.23e-46 PDB 3ZNZ "Crystal Structure Of Otulin Otu Domain (c129a) In Complex With Met1-di Ubiquitin" 100.00 152 100.00 100.00 4.37e-45 PDB 4A18 "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 1" 100.00 129 97.37 98.68 1.83e-44 PDB 4A19 "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 2" 100.00 129 97.37 98.68 1.83e-44 PDB 4A1B "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 3" 100.00 129 97.37 98.68 1.83e-44 PDB 4A1D "T.Thermophila 60s Ribosomal Subunit In Complex With Initiation Factor 6. This File Contains 26s Rrna And Proteins Of Molecule 4" 100.00 129 97.37 98.68 1.83e-44 PDB 4ADX "The Cryo-em Structure Of The Archaeal 50s Ribosomal Subunit In Complex With Initiation Factor 6" 100.00 129 97.37 98.68 1.83e-44 PDB 4AP4 "Rnf4 - Ubch5a - Ubiquitin Heterotrimeric Complex" 100.00 80 100.00 100.00 4.66e-46 PDB 4AUQ "Structure Of Birc7-Ubch5b-Ub Complex." 100.00 81 100.00 100.00 5.23e-46 PDB 4BBN "Nedd4 Hect-ub:ub Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 4BOS "Structure Of Otud2 Otu Domain In Complex With Ubiquitin K11- Linked Peptide" 100.00 76 100.00 100.00 4.45e-46 PDB 4BOZ "Structure Of Otud2 Otu Domain In Complex With K11-linked Di Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 4BVU "Structure Of Shigella Effector Ospg In Complex With Host Ubch5c-ubiquitin Conjugate" 100.00 76 100.00 100.00 4.45e-46 PDB 4CXC "Regulation Of The Mammalian Elongation Cycle By 40s Subunit Rolling: A Eukaryotic-specific Ribosome Rearrangement" 100.00 156 100.00 100.00 5.21e-46 PDB 4CXD "Regulation Of The Mammalian Elongation Cycle By 40s Subunit Rolling: A Eukaryotic-specific Ribosome Rearrangement" 100.00 128 100.00 100.00 3.09e-46 PDB 4DDG "Crystal Structure Of Human Otub1UBCH5B~UBUB" 100.00 76 100.00 100.00 4.45e-46 PDB 4DDI "Crystal Structure Of Human Otub1UBCH5B~UBUB" 100.00 76 100.00 100.00 4.45e-46 PDB 4DHJ "The Structure Of A Ceotub1 Ubiquitin Aldehyde Ubc13~ub Complex" 98.68 76 100.00 100.00 2.71e-45 PDB 4DHZ "The Structure Of HCEOTUB1-Ubiquitin Aldehyde-Ubc13~ub" 98.68 76 100.00 100.00 2.71e-45 PDB 4FJV "Crystal Structure Of Human Otubain2 And Ubiquitin Complex" 100.00 86 100.00 100.00 6.71e-46 PDB 4HXD "Diversity Of Ubiquitin And Isg15 Specificity Amongst Nairoviruses Viral Ovarian Tumor Domain Proteases" 98.68 75 100.00 100.00 2.91e-45 PDB 4I6N "Crystal Structure Of Trichinella Spiralis Uch37 Catalytic Domain Bound To Ubiquitin Vinyl Methyl Ester" 97.37 75 100.00 100.00 3.09e-44 PDB 4IG7 "Crystal Structure Of Trichinella Spiralis Uch37 Bound To Ubiquitin Vinyl Methyl Ester" 98.68 75 100.00 100.00 2.91e-45 PDB 4IUM "Equine Arteritis Virus Papain-like Protease 2 (plp2) Covalently Bound To Ubiquitin" 98.68 76 100.00 100.00 2.99e-45 PDB 4JIO "Bro1 V Domain And Ubiquitin" 100.00 76 98.68 98.68 3.30e-45 PDB 4JQW "Crystal Structure Of A Complex Of Nod1 Card And Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 4K1R "Crystal Structure Of Schizosaccharomyces Pombe Sst2 Catalytic Domain And Ubiquitin" 100.00 81 100.00 100.00 7.82e-46 PDB 4K7S "Crystal Structure Of Zn2-hub (human Ubiquitin) Adduct From A Solution 35 Mm Zinc Acetate/1.3 Mm Hub" 100.00 76 100.00 100.00 4.45e-46 PDB 4K7U "Crystal Structure Of Zn2.3-hub (human Ubiquitin) Adduct From A Solution 70 Mm Zinc Acetate/1.3 Mm Hub" 100.00 76 100.00 100.00 4.45e-46 PDB 4K7W "Crystal Structure Of Zn3-hub(human Ubiquitin) Adduct From A Solution 100 Mm Zinc Acetate/1.3 Mm Hub" 100.00 76 100.00 100.00 4.45e-46 PDB 4KSK "Gumby/fam105b In Complex With Ubiquitin" 100.00 80 100.00 100.00 4.66e-46 PDB 4KSL "Gumby/fam105b In Complex With Linear Di-ubiquitin" 100.00 156 100.00 100.00 4.31e-45 PDB 4KZX "Rabbit 40s Ribosomal Subunit In Complex With Eif1." 100.00 156 100.00 100.00 5.21e-46 PDB 4KZY "Rabbit 40s Ribosomal Subunit In Complex With Eif1 And Eif1a." 100.00 156 100.00 100.00 5.21e-46 PDB 4KZZ "Rabbit 40s Ribosomal Subunit In Complex With Mrna, Initiator Trna And Eif1a" 100.00 156 100.00 100.00 5.21e-46 PDB 4LCD "Structure Of An Rsp5xubxsna3 Complex: Mechanism Of Ubiquitin Ligation And Lysine Prioritization By A Hect E3" 97.37 83 100.00 100.00 1.22e-44 PDB 4LDT "The Structure Of H/ceotub1-ubiquitin Aldehyde-ubch5b~ub" 100.00 76 100.00 100.00 4.45e-46 PDB 4LJO "Structure Of An Active Ligase (hoip)/ubiquitin Transfer Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 4LJP "Structure Of An Active Ligase (hoip-h889a)/ubiquitin Transfer Complex" 100.00 76 100.00 100.00 4.45e-46 PDB 4M0W "Crystal Structure Of Sars-cov Papain-like Protease C112s Mutant In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 4MDK "Cdc34-ubiquitin-cc0651 Complex" 100.00 80 100.00 100.00 4.66e-46 PDB 4MM3 "Crystal Structure Of Sars-cov Papain-like Protease Plpro In Complex With Ubiquitin Aldehyde" 98.68 76 100.00 100.00 2.99e-45 PDB 4MSM "Crystal Structure Of Schizosaccharomyces Pombe Amsh-like Protease Sst2 E286a Mutant Bound To Ubiquitin" 100.00 81 100.00 100.00 7.82e-46 PDB 4MSQ "Crystal Structure Of Schizosaccharomyces Pombe Amsh-like Protease Sst2 Catalytic Domain Bound To Ubiquitin" 100.00 81 100.00 100.00 7.82e-46 PDB 4NQK "Structure Of An Ubiquitin Complex" 100.00 79 100.00 100.00 8.16e-46 PDB 4NQL "The Crystal Structure Of The Dub Domain Of Amsh Orthologue, Sst2 From S. Pombe, In Complex With Lysine 63-linked Diubiquitin" 100.00 77 100.00 100.00 5.05e-46 PDB 4P4H "Caught-in-action Signaling Complex Of Rig-i 2card Domain And Mavs Card Domain" 100.00 79 100.00 100.00 8.16e-46 PDB 4PIG "Crystal Structure Of The Ubiquitin K11s Mutant" 100.00 76 98.68 98.68 1.92e-45 PDB 4PIH "X-ray Crystal Structure Of The K33s Mutant Of Ubiquitin" 100.00 76 98.68 98.68 1.92e-45 PDB 4PIJ "X-ray Crystal Structure Of The K11s/k63s Double Mutant Of Ubiquitin" 98.68 75 97.33 97.33 5.38e-44 PDB 4PQT "Insights Into The Mechanism Of Deubiquitination By Jamm Deubiquitinases From Co-crystal Structures Of Enzyme With Substrate And" 100.00 81 100.00 100.00 7.82e-46 PDB 4RF0 "Crystal Structure Of The Middle-east Respiratory Syndrome Coronavirus Papain-like Protease In Complex With Ubiquitin (space Gro" 98.68 75 100.00 100.00 2.91e-45 PDB 4RF1 "Crystal Structure Of The Middle-east Respiratory Syndrome Coronavirus Papain-like Protease In Complex With Ubiquitin (space Gro" 98.68 75 100.00 100.00 2.91e-45 PDB 4UN2 "Crystal Structure Of The Uba Domain Of Dsk2 In Complex With Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 4UPX "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Pre-like State" 100.00 128 100.00 100.00 3.09e-46 PDB 4UQ1 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Post-like State" 100.00 128 100.00 100.00 3.09e-46 PDB 4UQ4 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Post-like State" 100.00 156 100.00 100.00 5.21e-46 PDB 4UQ5 "Mammalian 80s Hcv-ires Initiation Complex With Eif5b Pre-like State" 100.00 156 100.00 100.00 5.21e-46 PDB 4W20 "Structure Of The Mammalian 60s Ribosomal Subunit (this Entry Contains The Large Ribosomal Proteins)" 100.00 128 100.00 100.00 3.09e-46 PDB 4W22 "Structure Of The 80s Mammalian Ribosome Bound To Eef2 (this Entry Contains The Large Ribosomal Subunit Proteins)" 100.00 128 100.00 100.00 3.09e-46 PDB 4W23 "Structure Of The 80s Mammalian Ribosome Bound To Eef2 (this Entry Contains The Small Ribosomal Subunit)" 100.00 156 100.00 100.00 5.21e-46 PDB 4W25 "Structure Of The Idle Mammalian Ribosome-sec61 Complex (this Entry Contains The Large Ribosomal Subunit Proteins)" 100.00 128 100.00 100.00 3.09e-46 PDB 4W27 "Structure Of The Translating Mammalian Ribosome-sec61 Complex (this Entry Contains The Large Ribosomal Subunit Proteins)" 100.00 128 100.00 100.00 3.09e-46 PDB 4W28 "Structure Of The Translating Mammalian Ribosome-sec61 Complex (this Entry Contains The Small Ribosomal Subunit)" 100.00 156 100.00 100.00 5.21e-46 PDB 4WUR "The Crystal Structure Of The Mers-cov Papain-like Protease (c111s) With Human Ubiquitin" 100.00 76 100.00 100.00 4.45e-46 PDB 4WZP "Ser65 Phosphorylated Ubiquitin, Major Conformation" 100.00 76 98.68 98.68 3.16e-45 DBJ BAA03983 "polyubiquitin [Rattus norvegicus]" 100.00 305 100.00 100.00 2.21e-43 DBJ BAA09860 "polyubiquitin [Homo sapiens]" 100.00 611 98.68 98.68 1.04e-40 DBJ BAA11842 "ubiquitin [Cavia porcellus]" 100.00 311 100.00 100.00 2.40e-43 DBJ BAA11843 "ubiquitin extention protein [Cavia porcellus]" 100.00 156 100.00 100.00 5.21e-46 DBJ BAA23486 "polyubiquitin [Homo sapiens]" 100.00 609 98.68 98.68 5.60e-41 EMBL CAA25706 "unnamed protein product [Saccharomyces cerevisiae]" 50.00 191 100.00 100.00 2.98e-16 EMBL CAA26488 "unnamed protein product [Gallus gallus]" 100.00 157 98.68 98.68 2.66e-44 EMBL CAA28495 "ubiquitin [Homo sapiens]" 100.00 229 100.00 100.00 3.53e-44 EMBL CAA30183 "unnamed protein product [Dictyostelium discoideum]" 100.00 128 97.37 97.37 5.38e-44 EMBL CAA30815 "unnamed protein product [Cricetulus sp.]" 93.42 223 100.00 100.00 3.70e-40 GB AAA02769 "polyprotein [Bovine viral diarrhea virus 1-Osloss]" 98.68 3975 97.33 100.00 1.83e-39 GB AAA28154 "polyubiquitin [Caenorhabditis elegans]" 100.00 838 97.37 98.68 7.19e-40 GB AAA28997 "ubiquitin [Drosophila melanogaster]" 100.00 231 100.00 100.00 3.30e-44 GB AAA28998 "ubiquitin-hybrid protein precursor [Drosophila melanogaster]" 100.00 156 100.00 100.00 9.66e-46 GB AAA28999 "ubiquitin, partial [Drosophila melanogaster]" 100.00 76 100.00 100.00 4.45e-46 PIR I50437 "polyubiquitin 4 - chicken [Gallus gallus]" 100.00 305 100.00 100.00 2.21e-43 PIR I51568 "polyubiquitin - African clawed frog (fragment)" 100.00 167 100.00 100.00 6.83e-45 PIR I65237 "ubiquitin / ribosomal protein L40, cytosolic [validated] - rat" 100.00 128 100.00 100.00 3.09e-46 PIR JN0790 "ubiquitin/ribosomal protein CEP52 fusion protein - Leishmania major" 100.00 128 97.37 98.68 2.33e-45 PIR S13928 "ubiquitin precursor - chicken [Gallus gallus]" 100.00 229 100.00 100.00 3.64e-44 PRF 0412265A ubiquitin 98.68 75 98.67 98.67 1.22e-44 PRF 1101405A "ubiquitin precursor" 50.00 191 100.00 100.00 2.95e-16 PRF 1212243A "ubiquitin S1" 100.00 76 100.00 100.00 4.45e-46 PRF 1212243B "ubiquitin S5" 92.11 77 98.57 98.57 7.61e-41 PRF 1212243C "ubiquitin S3" 100.00 76 100.00 100.00 4.45e-46 REF NP_001005123 "ubiquitin A-52 residue ribosomal protein fusion product 1 [Xenopus (Silurana) tropicalis]" 100.00 128 100.00 100.00 3.09e-46 REF NP_001006688 "ubiquitin C [Xenopus (Silurana) tropicalis]" 100.00 609 100.00 100.00 9.87e-42 REF NP_001009117 "polyubiquitin-B [Pan troglodytes]" 100.00 229 100.00 100.00 3.53e-44 REF NP_001009202 "polyubiquitin-B [Ovis aries]" 100.00 305 98.68 100.00 3.89e-43 REF NP_001009286 "ubiquitin-60S ribosomal protein L40 [Ovis aries]" 100.00 128 100.00 100.00 3.09e-46 SP P0C273 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 100.00 128 100.00 100.00 3.09e-46 SP P0C275 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 100.00 128 100.00 100.00 3.09e-46 SP P0C276 "RecName: Full=Ubiquitin-60S ribosomal protein L40; AltName: Full=Ubiquitin A-52 residue ribosomal protein fusion product 1; Con" 100.00 128 100.00 100.00 3.09e-46 SP P0CG47 "RecName: Full=Polyubiquitin-B; Contains: RecName: Full=Ubiquitin; Flags: Precursor [Homo sapiens]" 100.00 229 100.00 100.00 3.53e-44 SP P0CG48 "RecName: Full=Polyubiquitin-C; Contains: RecName: Full=Ubiquitin; Flags: Precursor [Homo sapiens]" 100.00 685 100.00 100.00 1.45e-41 TPD FAA00319 "TPA: polyubiquitin [Cryptococcus neoformans var. neoformans B-3501A]" 100.00 456 97.37 98.68 7.53e-41 TPG DAA18802 "TPA: polyubiquitin [Bos taurus]" 100.00 305 100.00 100.00 2.33e-43 TPG DAA20663 "TPA: ubiquitin C [Bos taurus]" 98.68 314 98.67 100.00 8.85e-42 TPG DAA20672 "TPA: ubiquitin B-like [Bos taurus]" 100.00 77 98.68 98.68 3.32e-45 TPG DAA24675 "TPA: 40S ribosomal protein S27a [Bos taurus]" 100.00 156 100.00 100.00 5.21e-46 TPG DAA28295 "TPA: ubiquitin and ribosomal protein L40 [Bos taurus]" 100.00 128 100.00 100.00 3.09e-46 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $EKLF 'E. coli' 562 Bacteria . Escherichia coli $Ubiquitin 'E. coli' 562 Bacteria . Escherichia coli stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $EKLF 'recombinant technology' . Escherichia coli . pGEX-2T $Ubiquitin 'recombinant technology' . Escherichia coli . pET21b+ stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Ubiquitin 4 mM 'natural abundance' $EKLF 0.8 mM '[U-100% 15N]' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $EKLF 4 mM 'natural abundance' $Ubiquitin 0.8 mM '[U-100% 15N]' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $EKLF 4 mM 'natural abundance' $Ubiquitin 0.8 mM '[U-100% 13C; U-100% 15N]' D2O 100 % 'natural abundance' stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $Ubiquitin 4 mM 'natural abundance' 'phosphate buffer' 20 mM 'natural abundance' $EKLF 0.8 mM '[U-100% 13C; U-100% 15N]' D2O 100 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CNS _Saveframe_category software _Name CNS _Version . loop_ _Vendor _Address _Electronic_address 'Brunger, Adams, Clore, Gros, Nilges and Read' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model INOVA _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_3D_1H-15N_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_1 save_ save_3D_1H-13C_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_4 save_ save_3D_1H-15N_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_2 save_ save_3D_1H-13C_NOESY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-13C NOESY' _Sample_label $sample_3 save_ ####################### # Sample conditions # ####################### save_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 6.5 . pH temperature 300 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1_2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D 1H-15N NOESY' '3D 1H-13C NOESY' stop_ loop_ _Sample_label $sample_1 $sample_4 stop_ _Sample_conditions_label $conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name Ubiquitin _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 MET HA H 4.194 0.006 1 2 1 1 MET HB2 H 2.112 0.011 2 3 1 1 MET HB3 H 2.053 0.011 2 4 1 1 MET HG2 H 2.498 0.006 2 5 1 1 MET HG3 H 1.973 0.006 2 6 1 1 MET HE H 1.624 0.003 1 7 1 1 MET CA C 54.296 0.031 1 8 1 1 MET CB C 33.035 0.042 1 9 1 1 MET CG C 30.814 0.031 1 10 1 1 MET CE C 17.805 0.068 1 11 2 2 GLN H H 8.937 0.002 1 12 2 2 GLN HA H 5.239 0.008 1 13 2 2 GLN HB2 H 1.627 0.022 2 14 2 2 GLN HB3 H 1.816 0.008 2 15 2 2 GLN HG2 H 2.187 0.005 1 16 2 2 GLN HG3 H 2.187 0.005 1 17 2 2 GLN HE21 H 6.709 0.000 1 18 2 2 GLN HE22 H 7.700 0.000 1 19 2 2 GLN CA C 55.079 0.000 1 20 2 2 GLN CB C 30.742 0.006 1 21 2 2 GLN CG C 34.618 0.000 1 22 2 2 GLN N N 123.070 0.083 1 23 2 2 GLN NE2 N 112.103 0.001 1 24 3 3 ILE H H 8.275 0.020 1 25 3 3 ILE HA H 4.171 0.018 1 26 3 3 ILE HB H 1.754 0.015 1 27 3 3 ILE HG12 H 1.026 0.009 2 28 3 3 ILE HG13 H 0.800 0.014 2 29 3 3 ILE HG2 H 0.606 0.008 1 30 3 3 ILE HD1 H 0.566 0.006 1 31 3 3 ILE CA C 59.616 0.034 1 32 3 3 ILE CB C 42.232 0.000 1 33 3 3 ILE CG1 C 24.987 0.052 1 34 3 3 ILE CG2 C 17.926 0.018 1 35 3 3 ILE CD1 C 14.246 0.025 1 36 3 3 ILE N N 115.330 0.048 1 37 4 4 PHE H H 8.538 0.014 1 38 4 4 PHE HA H 5.570 0.014 1 39 4 4 PHE HB2 H 2.839 0.014 2 40 4 4 PHE HB3 H 3.013 0.013 2 41 4 4 PHE HD1 H 7.052 0.005 3 42 4 4 PHE HD2 H 7.052 0.005 3 43 4 4 PHE HE1 H 7.209 0.001 3 44 4 4 PHE HE2 H 7.209 0.001 3 45 4 4 PHE CA C 55.260 0.000 1 46 4 4 PHE CB C 41.233 0.019 1 47 4 4 PHE N N 118.730 0.000 1 48 5 5 VAL H H 9.312 0.003 1 49 5 5 VAL HA H 4.686 0.015 1 50 5 5 VAL HB H 1.899 0.008 1 51 5 5 VAL HG1 H 0.704 0.013 1 52 5 5 VAL HG2 H 0.672 0.007 1 53 5 5 VAL CA C 60.581 0.027 1 54 5 5 VAL CB C 34.159 0.008 1 55 5 5 VAL CG1 C 20.759 0.026 1 56 5 5 VAL CG2 C 22.627 0.027 1 57 5 5 VAL N N 121.902 0.039 1 58 6 6 LYS H H 8.925 0.002 1 59 6 6 LYS HA H 5.383 0.036 1 60 6 6 LYS HB2 H 1.289 0.008 2 61 6 6 LYS HB3 H 1.645 0.016 2 62 6 6 LYS HG2 H 1.253 0.013 2 63 6 6 LYS HG3 H 1.489 0.015 2 64 6 6 LYS HD2 H 1.574 0.005 1 65 6 6 LYS HD3 H 1.514 0.017 2 66 6 6 LYS HE2 H 2.854 0.004 1 67 6 6 LYS HE3 H 2.854 0.004 1 68 6 6 LYS CA C 54.410 0.031 1 69 6 6 LYS CB C 35.271 0.002 1 70 6 6 LYS CG C 25.214 0.007 1 71 6 6 LYS CD C 29.331 0.037 1 72 6 6 LYS CE C 41.811 0.016 1 73 6 6 LYS N N 128.033 0.061 1 74 7 7 THR H H 8.645 0.002 1 75 7 7 THR HA H 5.033 0.021 1 76 7 7 THR HB H 4.783 0.020 1 77 7 7 THR HG2 H 1.157 0.007 1 78 7 7 THR CA C 60.245 0.033 1 79 7 7 THR CB C 70.480 0.035 1 80 7 7 THR CG2 C 21.525 0.027 1 81 7 7 THR N N 114.076 0.000 1 82 8 8 LEU H H 9.240 0.003 1 83 8 8 LEU HA H 4.209 0.004 1 84 8 8 LEU HB2 H 1.677 0.005 2 85 8 8 LEU HB3 H 1.843 0.006 2 86 8 8 LEU HG H 1.784 0.006 1 87 8 8 LEU HD1 H 0.806 0.026 2 88 8 8 LEU HD2 H 0.918 0.005 2 89 8 8 LEU CA C 57.414 0.000 1 90 8 8 LEU CB C 41.875 0.001 1 91 8 8 LEU CG C 27.361 0.000 1 92 8 8 LEU CD1 C 24.032 0.080 2 93 8 8 LEU CD2 C 25.278 0.041 2 94 8 8 LEU N N 122.035 0.056 1 95 9 9 THR H H 7.551 0.002 1 96 9 9 THR HA H 4.368 0.005 1 97 9 9 THR HB H 4.540 0.005 1 98 9 9 THR HG2 H 1.192 0.006 1 99 9 9 THR CA C 61.199 0.048 1 100 9 9 THR CB C 68.857 0.048 1 101 9 9 THR CG2 C 21.987 0.000 1 102 9 9 THR N N 105.609 0.000 1 103 10 10 GLY H H 7.813 0.002 1 104 10 10 GLY HA2 H 3.583 0.009 2 105 10 10 GLY HA3 H 4.293 0.008 2 106 10 10 GLY CA C 45.321 0.001 1 107 10 10 GLY N N 109.211 0.000 1 108 11 11 LYS H H 7.286 0.002 1 109 11 11 LYS HA H 4.332 0.004 1 110 11 11 LYS HB2 H 1.783 0.019 2 111 11 11 LYS HB3 H 1.677 0.009 2 112 11 11 LYS HG2 H 1.218 0.011 2 113 11 11 LYS HG3 H 1.373 0.011 2 114 11 11 LYS HD2 H 1.582 0.010 1 115 11 11 LYS HD3 H 1.582 0.010 1 116 11 11 LYS HE2 H 2.882 0.011 1 117 11 11 LYS HE3 H 2.882 0.011 1 118 11 11 LYS CA C 56.212 0.000 1 119 11 11 LYS CB C 33.207 0.000 1 120 11 11 LYS CG C 25.018 0.035 1 121 11 11 LYS CD C 29.213 0.000 1 122 11 11 LYS CE C 41.800 0.000 1 123 11 11 LYS N N 121.934 0.000 1 124 12 12 THR H H 8.604 0.005 1 125 12 12 THR HA H 5.010 0.012 1 126 12 12 THR HB H 3.921 0.008 1 127 12 12 THR HG2 H 1.059 0.012 1 128 12 12 THR CA C 62.375 0.000 1 129 12 12 THR CB C 69.776 0.017 1 130 12 12 THR CG2 C 22.029 0.000 1 131 12 12 THR N N 120.682 0.023 1 132 13 13 ILE H H 9.624 0.003 1 133 13 13 ILE HA H 4.476 0.024 1 134 13 13 ILE HB H 1.838 0.010 1 135 13 13 ILE HG12 H 1.428 0.005 2 136 13 13 ILE HG13 H 1.056 0.010 2 137 13 13 ILE HG2 H 0.849 0.008 1 138 13 13 ILE HD1 H 0.693 0.008 1 139 13 13 ILE CA C 60.016 0.000 1 140 13 13 ILE CB C 40.824 0.031 1 141 13 13 ILE CG1 C 26.948 0.042 1 142 13 13 ILE CG2 C 17.849 0.000 1 143 13 13 ILE CD1 C 14.506 0.026 1 144 13 13 ILE N N 128.922 0.000 1 145 14 14 THR H H 8.745 0.002 1 146 14 14 THR HA H 4.923 0.009 1 147 14 14 THR HB H 4.004 0.005 1 148 14 14 THR HG2 H 1.103 0.007 1 149 14 14 THR CA C 61.860 0.034 1 150 14 14 THR CB C 69.361 0.042 1 151 14 14 THR CG2 C 21.784 0.037 1 152 14 14 THR N N 122.640 0.000 1 153 15 15 LEU H H 8.679 0.010 1 154 15 15 LEU HA H 4.744 0.010 1 155 15 15 LEU HB2 H 1.340 0.006 2 156 15 15 LEU HB3 H 1.184 0.009 2 157 15 15 LEU HG H 1.394 0.008 1 158 15 15 LEU HD1 H 0.741 0.005 1 159 15 15 LEU HD2 H 0.741 0.005 1 160 15 15 LEU CA C 52.682 0.037 1 161 15 15 LEU CB C 46.972 0.029 1 162 15 15 LEU CG C 26.809 0.000 1 163 15 15 LEU CD1 C 24.134 0.098 1 164 15 15 LEU CD2 C 24.131 0.098 1 165 15 15 LEU N N 125.401 0.000 1 166 16 16 GLU H H 8.093 0.002 1 167 16 16 GLU HA H 4.869 0.011 1 168 16 16 GLU HB2 H 1.809 0.009 2 169 16 16 GLU HB3 H 1.903 0.012 2 170 16 16 GLU HG2 H 2.243 0.019 2 171 16 16 GLU HG3 H 2.094 0.022 2 172 16 16 GLU CA C 54.789 0.000 1 173 16 16 GLU CB C 29.480 0.082 1 174 16 16 GLU CG C 35.086 0.001 1 175 16 16 GLU N N 122.589 0.005 1 176 17 17 VAL H H 8.926 0.002 1 177 17 17 VAL HA H 4.678 0.009 1 178 17 17 VAL HB H 2.310 0.012 1 179 17 17 VAL HG1 H 0.406 0.010 2 180 17 17 VAL HG2 H 0.686 0.010 2 181 17 17 VAL CA C 58.398 0.000 1 182 17 17 VAL CB C 36.355 0.000 1 183 17 17 VAL CG1 C 19.668 0.000 2 184 17 17 VAL CG2 C 22.324 0.040 2 185 17 17 VAL N N 117.736 0.000 1 186 18 18 GLU H H 8.634 0.002 1 187 18 18 GLU HA H 5.060 0.008 1 188 18 18 GLU HB2 H 2.157 0.006 2 189 18 18 GLU HB3 H 1.601 0.016 2 190 18 18 GLU HG2 H 2.246 0.004 2 191 18 18 GLU HG3 H 2.374 0.007 2 192 18 18 GLU CA C 52.540 0.047 1 193 18 18 GLU CB C 30.497 0.000 1 194 18 18 GLU CG C 34.586 0.026 1 195 18 18 GLU N N 119.472 0.000 1 196 19 19 PRO HA H 4.109 0.006 1 197 19 19 PRO HB2 H 1.983 0.006 2 198 19 19 PRO HB3 H 2.422 0.006 2 199 19 19 PRO HG2 H 2.190 0.005 2 200 19 19 PRO HG3 H 2.040 0.009 2 201 19 19 PRO HD2 H 3.786 0.008 2 202 19 19 PRO HD3 H 3.983 0.005 2 203 19 19 PRO CA C 65.322 0.000 1 204 19 19 PRO CB C 31.906 0.003 1 205 19 19 PRO CG C 28.048 0.000 1 206 19 19 PRO CD C 50.391 0.031 1 207 20 20 SER H H 7.005 0.005 1 208 20 20 SER HA H 4.338 0.007 1 209 20 20 SER HB2 H 4.123 0.010 2 210 20 20 SER HB3 H 3.757 0.006 2 211 20 20 SER CA C 57.240 0.000 1 212 20 20 SER CB C 63.280 0.001 1 213 20 20 SER N N 103.554 0.000 1 214 21 21 ASP H H 8.030 0.002 1 215 21 21 ASP HA H 4.663 0.009 1 216 21 21 ASP HB2 H 2.508 0.017 2 217 21 21 ASP HB3 H 2.926 0.015 2 218 21 21 ASP CA C 55.793 0.000 1 219 21 21 ASP CB C 40.837 0.003 1 220 21 21 ASP N N 124.274 0.040 1 221 22 22 THR H H 7.855 0.003 1 222 22 22 THR HA H 4.889 0.008 1 223 22 22 THR HB H 4.798 0.013 1 224 22 22 THR HG2 H 1.241 0.010 1 225 22 22 THR CA C 59.687 0.000 1 226 22 22 THR CB C 70.990 0.034 1 227 22 22 THR CG2 C 22.171 0.035 1 228 22 22 THR N N 109.319 0.039 1 229 23 23 ILE H H 8.504 0.002 1 230 23 23 ILE HA H 3.620 0.015 1 231 23 23 ILE HB H 2.540 0.005 1 232 23 23 ILE HG12 H 1.257 0.012 2 233 23 23 ILE HG13 H 1.878 0.007 2 234 23 23 ILE HG2 H 0.755 0.007 1 235 23 23 ILE HD1 H 0.551 0.006 1 236 23 23 ILE CA C 62.149 0.033 1 237 23 23 ILE CB C 34.389 0.000 1 238 23 23 ILE CG1 C 27.775 0.037 1 239 23 23 ILE CG2 C 18.003 0.037 1 240 23 23 ILE CD1 C 9.762 0.000 1 241 23 23 ILE N N 121.672 0.043 1 242 24 24 GLU HA H 3.879 0.016 1 243 24 24 GLU HB2 H 2.018 0.009 2 244 24 24 GLU HB3 H 2.018 0.009 2 245 24 24 GLU HG2 H 2.323 0.014 2 246 24 24 GLU HG3 H 2.431 0.011 2 247 24 24 GLU CA C 60.225 0.000 1 248 24 24 GLU CB C 28.328 0.009 1 249 24 24 GLU CG C 34.789 0.041 1 250 25 25 ASN H H 7.903 0.002 1 251 25 25 ASN HA H 4.505 0.004 1 252 25 25 ASN HB2 H 3.186 0.006 2 253 25 25 ASN HB3 H 2.846 0.013 2 254 25 25 ASN HD21 H 6.865 0.002 1 255 25 25 ASN HD22 H 7.812 0.000 1 256 25 25 ASN CA C 56.035 0.000 1 257 25 25 ASN CB C 38.329 0.032 1 258 25 25 ASN N N 121.465 0.000 1 259 25 25 ASN ND2 N 109.985 0.000 1 260 26 26 VAL H H 8.103 0.006 1 261 26 26 VAL HA H 3.367 0.009 1 262 26 26 VAL HB H 2.324 0.012 1 263 26 26 VAL HG1 H 0.674 0.007 2 264 26 26 VAL HG2 H 0.962 0.008 2 265 26 26 VAL CA C 67.668 0.000 1 266 26 26 VAL CB C 30.877 0.000 1 267 26 26 VAL CG1 C 21.493 0.030 2 268 26 26 VAL CG2 C 23.580 0.027 2 269 26 26 VAL N N 122.565 0.047 1 270 27 27 LYS H H 8.509 0.002 1 271 27 27 LYS HA H 4.558 0.007 1 272 27 27 LYS HB2 H 1.998 0.015 2 273 27 27 LYS HB3 H 1.406 0.009 2 274 27 27 LYS HG2 H 1.368 0.004 2 275 27 27 LYS HG3 H 1.549 0.009 2 276 27 27 LYS HD2 H 1.643 0.007 2 277 27 27 LYS HD3 H 1.644 0.007 2 278 27 27 LYS HE2 H 2.605 0.007 1 279 27 27 LYS HE3 H 2.604 0.007 1 280 27 27 LYS CA C 59.265 0.000 1 281 27 27 LYS CB C 33.713 0.011 1 282 27 27 LYS CG C 26.191 0.000 1 283 27 27 LYS CD C 30.229 0.180 1 284 27 27 LYS CE C 42.308 0.000 1 285 27 27 LYS N N 119.302 0.042 1 286 28 28 ALA H H 7.961 0.001 1 287 28 28 ALA HA H 4.136 0.008 1 288 28 28 ALA HB H 1.607 0.010 1 289 28 28 ALA CA C 55.340 0.013 1 290 28 28 ALA CB C 17.788 0.022 1 291 28 28 ALA N N 123.716 0.042 1 292 29 29 LYS H H 7.884 0.002 1 293 29 29 LYS HA H 4.178 0.010 1 294 29 29 LYS HB2 H 1.910 0.006 2 295 29 29 LYS HB3 H 2.123 0.008 2 296 29 29 LYS HG2 H 1.765 0.007 2 297 29 29 LYS HG3 H 1.583 0.006 2 298 29 29 LYS HD2 H 1.444 0.008 2 299 29 29 LYS HD3 H 1.766 0.006 2 300 29 29 LYS HE2 H 3.159 0.006 2 301 29 29 LYS HE3 H 2.956 0.003 2 302 29 29 LYS CA C 59.619 0.034 1 303 29 29 LYS CB C 33.293 0.104 1 304 29 29 LYS CG C 26.411 0.031 1 305 29 29 LYS CD C 29.880 0.067 1 306 29 29 LYS CE C 42.134 0.033 1 307 29 29 LYS N N 120.704 0.057 1 308 30 30 ILE H H 8.245 0.002 1 309 30 30 ILE HA H 3.480 0.010 1 310 30 30 ILE HB H 2.339 0.011 1 311 30 30 ILE HG12 H 1.991 0.009 1 312 30 30 ILE HG13 H 1.991 0.009 1 313 30 30 ILE HG2 H 0.672 0.009 1 314 30 30 ILE HD1 H 0.868 0.006 1 315 30 30 ILE CA C 66.081 0.023 1 316 30 30 ILE CB C 36.871 0.018 1 317 30 30 ILE CG1 C 31.083 0.039 1 318 30 30 ILE CG2 C 17.101 0.000 1 319 30 30 ILE CD1 C 15.185 0.027 1 320 30 30 ILE N N 121.823 0.044 1 321 31 31 GLN H H 8.502 0.001 1 322 31 31 GLN HA H 3.793 0.007 1 323 31 31 GLN HB2 H 1.956 0.009 2 324 31 31 GLN HB3 H 2.483 0.010 2 325 31 31 GLN HG2 H 1.907 0.005 2 326 31 31 GLN HG3 H 2.242 0.005 2 327 31 31 GLN HE21 H 6.785 0.000 1 328 31 31 GLN HE22 H 7.626 0.000 1 329 31 31 GLN CA C 59.982 0.000 1 330 31 31 GLN CB C 27.649 0.033 1 331 31 31 GLN CG C 33.641 0.030 1 332 31 31 GLN N N 123.747 0.000 1 333 31 31 GLN NE2 N 110.255 0.004 1 334 32 32 ASP H H 8.071 0.002 1 335 32 32 ASP HA H 4.312 0.013 1 336 32 32 ASP HB2 H 2.846 0.019 2 337 32 32 ASP HB3 H 2.753 0.018 2 338 32 32 ASP CA C 57.220 0.024 1 339 32 32 ASP CB C 40.536 0.058 1 340 32 32 ASP N N 120.236 0.044 1 341 33 33 LYS H H 7.445 0.002 1 342 33 33 LYS HA H 4.296 0.013 1 343 33 33 LYS HB2 H 1.830 0.009 2 344 33 33 LYS HB3 H 1.968 0.005 2 345 33 33 LYS HG2 H 1.581 0.005 1 346 33 33 LYS HG3 H 1.581 0.005 1 347 33 33 LYS HD2 H 1.687 0.005 1 348 33 33 LYS HD3 H 1.687 0.005 1 349 33 33 LYS HE2 H 3.130 0.013 1 350 33 33 LYS HE3 H 3.130 0.013 1 351 33 33 LYS CA C 58.018 0.000 1 352 33 33 LYS CB C 34.135 0.000 1 353 33 33 LYS CG C 25.246 0.028 1 354 33 33 LYS CD C 28.867 0.034 1 355 33 33 LYS CE C 42.030 0.024 1 356 33 33 LYS N N 115.876 0.044 1 357 34 34 GLU H H 8.665 0.002 1 358 34 34 GLU HA H 4.603 0.015 1 359 34 34 GLU HB2 H 2.263 0.008 2 360 34 34 GLU HB3 H 1.665 0.015 2 361 34 34 GLU HG2 H 2.155 0.008 1 362 34 34 GLU HG3 H 2.155 0.008 1 363 34 34 GLU CA C 55.109 0.000 1 364 34 34 GLU CB C 32.709 0.000 1 365 34 34 GLU CG C 35.242 0.000 1 366 34 34 GLU N N 114.203 0.000 1 367 35 35 GLY H H 8.478 0.002 1 368 35 35 GLY HA2 H 4.114 0.008 2 369 35 35 GLY HA3 H 3.880 0.008 2 370 35 35 GLY CA C 45.970 0.000 1 371 35 35 GLY N N 109.243 0.054 1 372 36 36 ILE H H 6.114 0.001 1 373 36 36 ILE HA H 4.348 0.008 1 374 36 36 ILE HB H 1.382 0.010 1 375 36 36 ILE HG12 H 1.339 0.005 2 376 36 36 ILE HG13 H 1.010 0.010 2 377 36 36 ILE HG2 H 0.886 0.004 1 378 36 36 ILE HD1 H 0.731 0.004 1 379 36 36 ILE CA C 57.631 0.036 1 380 36 36 ILE CB C 40.385 0.033 1 381 36 36 ILE CG1 C 26.885 0.035 1 382 36 36 ILE CG2 C 17.778 0.034 1 383 36 36 ILE CD1 C 13.621 0.000 1 384 36 36 ILE N N 120.726 0.065 1 385 37 37 PRO HA H 4.606 0.004 1 386 37 37 PRO HB2 H 2.395 0.007 2 387 37 37 PRO HB3 H 1.938 0.007 2 388 37 37 PRO HG2 H 2.021 0.004 2 389 37 37 PRO HG3 H 2.085 0.009 2 390 37 37 PRO HD2 H 3.520 0.005 2 391 37 37 PRO HD3 H 4.160 0.003 2 392 37 37 PRO CA C 61.558 0.000 1 393 37 37 PRO CB C 31.759 0.034 1 394 37 37 PRO CG C 28.306 0.013 1 395 37 37 PRO CD C 51.040 0.011 1 396 38 38 PRO HA H 4.097 0.005 1 397 38 38 PRO HB2 H 2.209 0.010 2 398 38 38 PRO HB3 H 2.021 0.010 2 399 38 38 PRO HG2 H 2.166 0.005 2 400 38 38 PRO HG3 H 1.599 0.008 2 401 38 38 PRO HD2 H 3.733 0.005 1 402 38 38 PRO HD3 H 3.732 0.005 1 403 38 38 PRO CA C 66.022 0.033 1 404 38 38 PRO CB C 32.892 0.000 1 405 38 38 PRO CG C 27.581 0.034 1 406 38 38 PRO CD C 51.140 0.000 1 407 39 39 ASP H H 8.512 0.002 1 408 39 39 ASP HA H 4.394 0.012 1 409 39 39 ASP HB2 H 2.776 0.014 2 410 39 39 ASP HB3 H 2.668 0.013 2 411 39 39 ASP CA C 55.609 0.000 1 412 39 39 ASP CB C 39.411 0.071 1 413 39 39 ASP N N 113.783 0.000 1 414 40 40 GLN H H 7.774 0.002 1 415 40 40 GLN HA H 4.438 0.015 1 416 40 40 GLN HB2 H 1.812 0.012 2 417 40 40 GLN HB3 H 2.454 0.004 2 418 40 40 GLN HG2 H 2.378 0.005 1 419 40 40 GLN HG3 H 2.378 0.005 1 420 40 40 GLN HE21 H 6.705 0.000 1 421 40 40 GLN HE22 H 7.640 0.000 1 422 40 40 GLN CA C 55.563 0.037 1 423 40 40 GLN CB C 30.111 0.004 1 424 40 40 GLN CG C 34.182 0.009 1 425 40 40 GLN N N 117.107 0.056 1 426 40 40 GLN NE2 N 110.920 0.002 1 427 41 41 GLN H H 7.455 0.002 1 428 41 41 GLN HA H 4.206 0.013 1 429 41 41 GLN HB2 H 1.954 0.012 2 430 41 41 GLN HB3 H 1.868 0.007 2 431 41 41 GLN HG2 H 1.610 0.007 2 432 41 41 GLN HG3 H 2.496 0.005 2 433 41 41 GLN CA C 56.537 0.000 1 434 41 41 GLN CB C 31.524 0.037 1 435 41 41 GLN CG C 33.398 0.030 1 436 41 41 GLN N N 118.003 0.043 1 437 42 42 ARG H H 8.429 0.001 1 438 42 42 ARG HA H 4.477 0.011 1 439 42 42 ARG HB2 H 1.573 0.009 2 440 42 42 ARG HB3 H 1.720 0.007 2 441 42 42 ARG HG2 H 1.402 0.010 2 442 42 42 ARG HG3 H 1.472 0.008 2 443 42 42 ARG HD2 H 2.993 0.005 2 444 42 42 ARG HD3 H 3.083 0.006 2 445 42 42 ARG CA C 54.894 0.034 1 446 42 42 ARG CB C 31.706 0.002 1 447 42 42 ARG CG C 26.895 0.035 1 448 42 42 ARG CD C 43.561 0.002 1 449 42 42 ARG N N 123.058 0.045 1 450 43 43 LEU H H 8.756 0.002 1 451 43 43 LEU HA H 5.313 0.011 1 452 43 43 LEU HB2 H 1.117 0.007 2 453 43 43 LEU HB3 H 1.547 0.013 2 454 43 43 LEU HG H 1.431 0.011 1 455 43 43 LEU HD1 H 0.775 0.014 2 456 43 43 LEU HD2 H 0.738 0.009 2 457 43 43 LEU CA C 52.972 0.019 1 458 43 43 LEU CB C 45.659 0.029 1 459 43 43 LEU CG C 27.359 0.147 1 460 43 43 LEU CD1 C 26.476 0.040 2 461 43 43 LEU CD2 C 24.310 0.000 2 462 43 43 LEU N N 124.449 0.000 1 463 44 44 ILE H H 9.103 0.002 1 464 44 44 ILE HA H 4.964 0.005 1 465 44 44 ILE HB H 1.620 0.005 1 466 44 44 ILE HG12 H 1.254 0.006 2 467 44 44 ILE HG13 H 0.996 0.005 2 468 44 44 ILE HG2 H 0.613 0.005 1 469 44 44 ILE HD1 H 0.604 0.004 1 470 44 44 ILE CA C 58.735 0.061 1 471 44 44 ILE CB C 41.312 0.037 1 472 44 44 ILE CG1 C 28.096 0.007 1 473 44 44 ILE CG2 C 17.679 0.015 1 474 44 44 ILE CD1 C 13.086 0.004 1 475 44 44 ILE N N 123.074 0.054 1 476 45 45 PHE H H 8.775 0.003 1 477 45 45 PHE HA H 5.010 0.005 1 478 45 45 PHE HB2 H 2.768 0.008 2 479 45 45 PHE HB3 H 3.017 0.003 2 480 45 45 PHE HD1 H 7.326 0.004 3 481 45 45 PHE HD2 H 7.326 0.004 3 482 45 45 PHE HE1 H 7.510 0.003 3 483 45 45 PHE HE2 H 7.510 0.003 3 484 45 45 PHE CA C 56.925 0.028 1 485 45 45 PHE CB C 43.954 0.014 1 486 45 45 PHE N N 125.292 0.086 1 487 46 46 ALA H H 8.899 0.000 1 488 46 46 ALA HA H 3.629 0.005 1 489 46 46 ALA HB H 0.878 0.005 1 490 46 46 ALA CA C 52.446 0.000 1 491 46 46 ALA CB C 16.649 0.003 1 492 46 46 ALA N N 132.792 0.000 1 493 47 47 GLY H H 7.917 0.004 1 494 47 47 GLY HA2 H 3.995 0.010 2 495 47 47 GLY HA3 H 3.336 0.006 2 496 47 47 GLY CA C 45.290 0.047 1 497 47 47 GLY N N 102.277 0.000 1 498 48 48 LYS H H 7.823 0.002 1 499 48 48 LYS HA H 4.622 0.013 1 500 48 48 LYS HB2 H 1.861 0.008 2 501 48 48 LYS HB3 H 1.861 0.008 2 502 48 48 LYS HG2 H 1.495 0.013 1 503 48 48 LYS HG3 H 1.495 0.013 1 504 48 48 LYS HD2 H 1.838 0.010 1 505 48 48 LYS HD3 H 1.838 0.009 1 506 48 48 LYS HE2 H 3.134 0.004 1 507 48 48 LYS HE3 H 3.134 0.004 1 508 48 48 LYS CA C 54.408 0.000 1 509 48 48 LYS CB C 34.638 0.000 1 510 48 48 LYS CG C 24.527 0.000 1 511 48 48 LYS CD C 29.014 0.039 1 512 48 48 LYS CE C 42.036 0.000 1 513 48 48 LYS N N 121.030 0.000 1 514 49 49 GLN H H 8.667 0.002 1 515 49 49 GLN HA H 4.536 0.021 1 516 49 49 GLN HB2 H 1.965 0.016 1 517 49 49 GLN HB3 H 1.965 0.016 1 518 49 49 GLN HG2 H 2.199 0.011 2 519 49 49 GLN HG3 H 2.200 0.009 2 520 49 49 GLN HE21 H 6.845 0.000 1 521 49 49 GLN HE22 H 7.611 0.000 1 522 49 49 GLN CA C 55.584 0.035 1 523 49 49 GLN CB C 29.159 0.000 1 524 49 49 GLN CG C 34.785 0.000 1 525 49 49 GLN N N 122.971 0.000 1 526 49 49 GLN NE2 N 112.192 0.000 1 527 50 50 LEU H H 8.533 0.001 1 528 50 50 LEU HA H 4.052 0.007 1 529 50 50 LEU HB2 H 1.001 0.013 2 530 50 50 LEU HB3 H 1.458 0.012 2 531 50 50 LEU HG H 1.466 0.017 1 532 50 50 LEU HD1 H -0.184 0.012 2 533 50 50 LEU HD2 H 0.493 0.007 2 534 50 50 LEU CA C 54.158 0.033 1 535 50 50 LEU CB C 41.315 0.075 1 536 50 50 LEU CG C 25.922 0.075 1 537 50 50 LEU CD1 C 19.627 0.020 2 538 50 50 LEU CD2 C 25.922 0.026 2 539 50 50 LEU N N 125.868 0.038 1 540 51 51 GLU H H 8.374 0.001 1 541 51 51 GLU HA H 4.454 0.009 1 542 51 51 GLU HB2 H 1.964 0.014 2 543 51 51 GLU HB3 H 2.215 0.017 2 544 51 51 GLU HG2 H 2.341 0.022 2 545 51 51 GLU HG3 H 2.437 0.019 2 546 51 51 GLU CA C 55.812 0.036 1 547 51 51 GLU CB C 31.537 0.042 1 548 51 51 GLU CG C 36.155 0.001 1 549 51 51 GLU N N 123.494 0.049 1 550 52 52 ASP H H 8.144 0.001 1 551 52 52 ASP HA H 4.339 0.005 1 552 52 52 ASP HB2 H 2.645 0.021 2 553 52 52 ASP HB3 H 2.515 0.014 2 554 52 52 ASP CA C 56.722 0.032 1 555 52 52 ASP CB C 40.799 0.002 1 556 52 52 ASP N N 120.831 0.046 1 557 53 53 GLY HA2 H 3.918 0.013 2 558 53 53 GLY HA3 H 4.128 0.000 2 559 53 53 GLY CA C 45.131 0.007 1 560 54 54 ARG H H 7.440 0.002 1 561 54 54 ARG HA H 4.670 0.004 1 562 54 54 ARG HB2 H 2.040 0.012 2 563 54 54 ARG HB3 H 2.215 0.010 2 564 54 54 ARG HG2 H 1.599 0.009 2 565 54 54 ARG HG3 H 1.805 0.009 2 566 54 54 ARG HD2 H 3.125 0.005 2 567 54 54 ARG HD3 H 3.047 0.004 2 568 54 54 ARG CA C 54.395 0.000 1 569 54 54 ARG CB C 32.672 0.001 1 570 54 54 ARG CG C 27.554 0.007 1 571 54 54 ARG CD C 42.708 0.032 1 572 54 54 ARG N N 119.697 0.052 1 573 55 55 THR H H 8.795 0.001 1 574 55 55 THR HA H 5.208 0.011 1 575 55 55 THR HB H 4.517 0.017 1 576 55 55 THR HG2 H 1.102 0.009 1 577 55 55 THR CA C 59.718 0.000 1 578 55 55 THR CB C 72.193 0.000 1 579 55 55 THR CG2 C 22.275 0.000 1 580 55 55 THR N N 109.075 0.050 1 581 56 56 LEU H H 8.122 0.009 1 582 56 56 LEU HA H 4.038 0.009 1 583 56 56 LEU HB2 H 1.193 0.011 2 584 56 56 LEU HB3 H 2.089 0.010 2 585 56 56 LEU HG H 1.695 0.008 1 586 56 56 LEU HD1 H 0.602 0.008 2 587 56 56 LEU HD2 H 0.729 0.008 2 588 56 56 LEU CA C 58.715 0.000 1 589 56 56 LEU CB C 40.328 0.001 1 590 56 56 LEU CG C 26.830 0.000 1 591 56 56 LEU CD1 C 23.346 0.000 2 592 56 56 LEU CD2 C 26.901 0.000 2 593 56 56 LEU N N 118.357 0.046 1 594 57 57 SER H H 8.449 0.001 1 595 57 57 SER HA H 4.219 0.010 1 596 57 57 SER HB2 H 3.725 0.020 2 597 57 57 SER HB3 H 3.815 0.009 2 598 57 57 SER CA C 60.805 0.071 1 599 57 57 SER CB C 62.300 0.031 1 600 57 57 SER N N 113.781 0.043 1 601 58 58 ASP H H 7.918 0.001 1 602 58 58 ASP HA H 4.254 0.014 1 603 58 58 ASP HB2 H 2.987 0.016 2 604 58 58 ASP HB3 H 2.258 0.016 2 605 58 58 ASP CA C 57.213 0.000 1 606 58 58 ASP CB C 40.180 0.002 1 607 58 58 ASP N N 124.902 0.046 1 608 59 59 TYR H H 7.222 0.002 1 609 59 59 TYR HA H 4.630 0.014 1 610 59 59 TYR HB2 H 2.507 0.014 2 611 59 59 TYR HB3 H 3.444 0.012 2 612 59 59 TYR HD1 H 6.868 0.000 3 613 59 59 TYR HD2 H 6.868 0.000 3 614 59 59 TYR CA C 58.172 0.042 1 615 59 59 TYR CB C 39.929 0.000 1 616 59 59 TYR N N 116.058 0.051 1 617 60 60 ASN H H 8.127 0.001 1 618 60 60 ASN HA H 4.323 0.009 1 619 60 60 ASN HB2 H 2.761 0.005 2 620 60 60 ASN HB3 H 3.284 0.007 2 621 60 60 ASN HD21 H 7.522 0.000 1 622 60 60 ASN HD22 H 6.788 0.000 1 623 60 60 ASN CA C 53.935 0.033 1 624 60 60 ASN CB C 37.388 0.009 1 625 60 60 ASN N N 116.081 0.058 1 626 60 60 ASN ND2 N 111.643 0.003 1 627 61 61 ILE H H 7.231 0.002 1 628 61 61 ILE HA H 3.355 0.010 1 629 61 61 ILE HB H 1.373 0.010 1 630 61 61 ILE HG12 H 1.092 0.013 2 631 61 61 ILE HG13 H -0.399 0.026 2 632 61 61 ILE HG2 H 0.450 0.008 1 633 61 61 ILE HD1 H 0.389 0.008 1 634 61 61 ILE CA C 62.317 0.035 1 635 61 61 ILE CB C 36.635 0.032 1 636 61 61 ILE CG1 C 28.284 0.046 1 637 61 61 ILE CG2 C 17.300 0.025 1 638 61 61 ILE CD1 C 14.577 0.000 1 639 61 61 ILE N N 119.287 0.076 1 640 62 62 GLN H H 7.606 0.001 1 641 62 62 GLN HA H 4.466 0.008 1 642 62 62 GLN HB2 H 1.865 0.009 2 643 62 62 GLN HB3 H 2.224 0.008 2 644 62 62 GLN HG2 H 2.274 0.012 2 645 62 62 GLN HG3 H 2.333 0.013 2 646 62 62 GLN HE21 H 6.787 0.000 1 647 62 62 GLN HE22 H 7.260 0.000 1 648 62 62 GLN CA C 53.437 0.031 1 649 62 62 GLN CB C 31.535 0.044 1 650 62 62 GLN CG C 33.377 0.000 1 651 62 62 GLN N N 125.246 0.067 1 652 62 62 GLN NE2 N 112.502 0.001 1 653 63 63 LYS H H 8.446 0.001 1 654 63 63 LYS HA H 3.960 0.012 1 655 63 63 LYS HB2 H 2.033 0.010 2 656 63 63 LYS HB3 H 1.874 0.011 2 657 63 63 LYS HG2 H 1.477 0.011 1 658 63 63 LYS HG3 H 1.477 0.011 1 659 63 63 LYS HD2 H 1.714 0.008 2 660 63 63 LYS HD3 H 1.713 0.009 2 661 63 63 LYS HE2 H 3.010 0.003 1 662 63 63 LYS HE3 H 3.010 0.003 1 663 63 63 LYS CA C 57.636 0.025 1 664 63 63 LYS CB C 32.631 0.013 1 665 63 63 LYS CG C 23.911 0.033 1 666 63 63 LYS CD C 29.648 0.033 1 667 63 63 LYS CE C 41.848 0.003 1 668 63 63 LYS N N 120.856 0.045 1 669 64 64 GLU H H 9.273 0.002 1 670 64 64 GLU HA H 3.397 0.037 1 671 64 64 GLU HB2 H 2.413 0.011 2 672 64 64 GLU HB3 H 2.547 0.017 2 673 64 64 GLU HG2 H 2.325 0.009 2 674 64 64 GLU HG3 H 2.223 0.007 2 675 64 64 GLU CA C 57.803 0.037 1 676 64 64 GLU CB C 25.935 0.001 1 677 64 64 GLU CG C 36.235 0.033 1 678 64 64 GLU N N 115.065 0.068 1 679 65 65 SER H H 7.645 0.002 1 680 65 65 SER HA H 4.603 0.006 1 681 65 65 SER HB2 H 3.617 0.009 2 682 65 65 SER HB3 H 3.863 0.003 2 683 65 65 SER CA C 60.842 0.000 1 684 65 65 SER CB C 64.898 0.023 1 685 65 65 SER N N 115.131 0.111 1 686 66 66 THR H H 8.672 0.002 1 687 66 66 THR HA H 5.232 0.002 1 688 66 66 THR HB H 4.027 0.015 1 689 66 66 THR HG2 H 0.864 0.012 1 690 66 66 THR CA C 62.301 0.000 1 691 66 66 THR CB C 69.942 0.000 1 692 66 66 THR CG2 C 21.555 0.014 1 693 66 66 THR N N 117.761 0.051 1 694 67 67 LEU H H 9.375 0.004 1 695 67 67 LEU HA H 5.007 0.006 1 696 67 67 LEU HB2 H 1.575 0.007 1 697 67 67 LEU HB3 H 1.575 0.007 1 698 67 67 LEU HG H 1.742 0.014 1 699 67 67 LEU HD1 H 0.634 0.006 2 700 67 67 LEU HD2 H 0.662 0.007 2 701 67 67 LEU CA C 53.709 0.037 1 702 67 67 LEU CB C 44.172 0.033 1 703 67 67 LEU CG C 29.480 0.037 1 704 67 67 LEU CD1 C 24.877 0.024 2 705 67 67 LEU CD2 C 25.290 0.003 2 706 67 67 LEU N N 128.001 0.052 1 707 68 68 HIS H H 9.091 0.007 1 708 68 68 HIS HA H 5.173 0.009 1 709 68 68 HIS HB2 H 2.944 0.007 2 710 68 68 HIS HB3 H 2.832 0.005 2 711 68 68 HIS HD2 H 8.811 0.004 1 712 68 68 HIS CA C 54.839 0.058 1 713 68 68 HIS CB C 30.650 0.000 1 714 68 68 HIS N N 119.380 0.053 1 715 69 69 LEU H H 8.422 0.003 1 716 69 69 LEU HA H 5.168 0.009 1 717 69 69 LEU HB2 H 1.564 0.010 2 718 69 69 LEU HB3 H 1.059 0.009 2 719 69 69 LEU HG H 1.311 0.007 1 720 69 69 LEU HD1 H 0.710 0.005 2 721 69 69 LEU HD2 H 0.835 0.006 2 722 69 69 LEU CA C 53.739 0.039 1 723 69 69 LEU CB C 44.323 0.047 1 724 69 69 LEU CG C 27.568 0.034 1 725 69 69 LEU CD1 C 26.228 0.000 2 726 69 69 LEU CD2 C 23.903 0.061 2 727 69 69 LEU N N 124.184 0.000 1 728 70 70 VAL H H 9.128 0.001 1 729 70 70 VAL HA H 4.320 0.004 1 730 70 70 VAL HB H 1.963 0.005 1 731 70 70 VAL HG1 H 0.931 0.004 2 732 70 70 VAL HG2 H 0.827 0.005 2 733 70 70 VAL CA C 60.655 0.029 1 734 70 70 VAL CB C 34.782 0.031 1 735 70 70 VAL CG1 C 21.526 0.026 2 736 70 70 VAL CG2 C 21.031 0.003 2 737 70 70 VAL N N 127.044 0.000 1 738 71 71 LEU H H 8.153 0.002 1 739 71 71 LEU HA H 5.058 0.005 1 740 71 71 LEU HB2 H 1.498 0.009 2 741 71 71 LEU HB3 H 1.643 0.011 2 742 71 71 LEU HG H 1.634 0.006 1 743 71 71 LEU HD1 H 0.824 0.006 2 744 71 71 LEU HD2 H 0.915 0.005 2 745 71 71 LEU CA C 53.816 0.000 1 746 71 71 LEU CB C 42.719 0.042 1 747 71 71 LEU CG C 27.633 0.039 1 748 71 71 LEU CD1 C 24.059 0.048 2 749 71 71 LEU CD2 C 25.095 0.036 2 750 71 71 LEU N N 124.079 0.000 1 751 72 72 ARG H H 8.574 0.001 1 752 72 72 ARG HA H 4.297 0.010 1 753 72 72 ARG HB2 H 1.516 0.004 2 754 72 72 ARG HB3 H 1.749 0.014 2 755 72 72 ARG HG2 H 1.508 0.007 2 756 72 72 ARG HG3 H 1.509 0.008 2 757 72 72 ARG HD2 H 3.122 0.004 1 758 72 72 ARG HD3 H 3.122 0.004 1 759 72 72 ARG CA C 55.535 0.000 1 760 72 72 ARG CB C 31.330 0.035 1 761 72 72 ARG CG C 27.321 0.002 1 762 72 72 ARG CD C 43.383 0.000 1 763 72 72 ARG N N 123.329 0.000 1 764 73 73 LEU H H 8.340 0.001 1 765 73 73 LEU HA H 4.365 0.007 1 766 73 73 LEU HB2 H 1.532 0.011 2 767 73 73 LEU HB3 H 1.612 0.013 2 768 73 73 LEU HG H 1.601 0.009 1 769 73 73 LEU HD1 H 0.883 0.004 2 770 73 73 LEU HD2 H 0.828 0.005 2 771 73 73 LEU CA C 54.856 0.000 1 772 73 73 LEU CB C 42.498 0.001 1 773 73 73 LEU CG C 27.090 0.042 1 774 73 73 LEU CD1 C 25.026 0.036 2 775 73 73 LEU CD2 C 23.371 0.083 2 776 73 73 LEU N N 124.213 0.000 1 777 74 74 ARG H H 8.390 0.001 1 778 74 74 ARG HA H 4.278 0.007 1 779 74 74 ARG HB2 H 1.842 0.008 2 780 74 74 ARG HB3 H 1.758 0.010 2 781 74 74 ARG HG2 H 1.608 0.012 2 782 74 74 ARG HG3 H 1.610 0.010 2 783 74 74 ARG HD2 H 3.163 0.006 1 784 74 74 ARG HD3 H 3.163 0.006 1 785 74 74 ARG CA C 56.259 0.034 1 786 74 74 ARG CB C 30.881 0.043 1 787 74 74 ARG CG C 27.087 0.000 1 788 74 74 ARG CD C 43.154 0.034 1 789 74 74 ARG N N 121.782 0.000 1 790 75 75 GLY H H 8.437 0.001 1 791 75 75 GLY HA2 H 3.918 0.001 1 792 75 75 GLY HA3 H 3.918 0.001 1 793 75 75 GLY N N 110.997 0.000 1 794 76 76 GLY H H 7.920 0.001 1 795 76 76 GLY HA2 H 3.759 0.000 2 796 76 76 GLY HA3 H 3.696 0.000 2 797 76 76 GLY N N 115.215 0.000 1 stop_ save_ save_assigned_chem_shift_list_1_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '3D 1H-15N NOESY' '3D 1H-13C NOESY' stop_ loop_ _Sample_label $sample_1 $sample_4 stop_ _Sample_conditions_label $conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name EKLF _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 22 1 ASP HA H 4.680 0.006 1 2 22 1 ASP HB2 H 2.708 0.007 1 3 22 1 ASP HB3 H 2.708 0.007 1 4 22 1 ASP H H 8.373 0.001 1 5 22 1 ASP C C 176.435 0.000 1 6 22 1 ASP CA C 54.093 0.127 1 7 22 1 ASP CB C 41.249 0.071 1 8 22 1 ASP N N 119.667 0.000 1 9 23 2 THR H H 7.932 0.002 1 10 23 2 THR HA H 4.394 0.008 1 11 23 2 THR HB H 4.326 0.004 1 12 23 2 THR HG2 H 1.204 0.006 1 13 23 2 THR C C 174.827 0.000 1 14 23 2 THR CA C 61.570 0.033 1 15 23 2 THR CB C 69.954 0.038 1 16 23 2 THR CG2 C 21.662 0.037 1 17 23 2 THR N N 112.528 0.017 1 18 24 3 GLN H H 8.493 0.004 1 19 24 3 GLN HA H 4.230 0.010 1 20 24 3 GLN HB2 H 2.135 0.003 2 21 24 3 GLN HB3 H 2.031 0.005 2 22 24 3 GLN HG2 H 2.325 0.005 1 23 24 3 GLN HG3 H 2.325 0.005 1 24 24 3 GLN HE21 H 6.778 0.000 1 25 24 3 GLN HE22 H 7.564 0.000 1 26 24 3 GLN C C 176.231 0.000 1 27 24 3 GLN CA C 57.066 0.044 1 28 24 3 GLN CB C 29.124 0.031 1 29 24 3 GLN CG C 33.854 0.041 1 30 24 3 GLN N N 122.034 0.011 1 31 24 3 GLN NE2 N 112.060 0.009 1 32 25 4 ASP H H 8.350 0.003 1 33 25 4 ASP HA H 4.504 0.006 1 34 25 4 ASP HB2 H 2.637 0.006 1 35 25 4 ASP HB3 H 2.637 0.006 1 36 25 4 ASP C C 176.888 0.000 1 37 25 4 ASP CA C 55.704 0.030 1 38 25 4 ASP CB C 41.340 0.046 1 39 25 4 ASP N N 120.475 0.001 1 40 26 5 ASP H H 8.222 0.001 1 41 26 5 ASP HA H 4.465 0.006 1 42 26 5 ASP HB2 H 2.625 0.005 1 43 26 5 ASP HB3 H 2.625 0.005 1 44 26 5 ASP C C 177.030 0.000 1 45 26 5 ASP CA C 55.881 0.026 1 46 26 5 ASP CB C 40.723 0.036 1 47 26 5 ASP N N 120.312 0.033 1 48 27 6 PHE H H 8.231 0.007 1 49 27 6 PHE HA H 4.261 0.007 1 50 27 6 PHE HB2 H 3.083 0.006 2 51 27 6 PHE HB3 H 3.015 0.005 2 52 27 6 PHE HD1 H 7.057 0.010 3 53 27 6 PHE HD2 H 7.057 0.010 3 54 27 6 PHE HE1 H 7.231 0.012 3 55 27 6 PHE HE2 H 7.231 0.012 3 56 27 6 PHE C C 177.016 0.000 1 57 27 6 PHE CA C 60.513 0.106 1 58 27 6 PHE CB C 39.001 0.027 1 59 27 6 PHE CD1 C 131.739 0.000 3 60 27 6 PHE CD2 C 131.739 0.000 3 61 27 6 PHE CE1 C 131.357 0.000 3 62 27 6 PHE CE2 C 131.357 0.000 3 63 27 6 PHE N N 120.994 0.052 1 64 28 7 LEU H H 8.035 0.002 1 65 28 7 LEU HA H 4.055 0.004 1 66 28 7 LEU HB2 H 1.538 0.004 2 67 28 7 LEU HB3 H 1.742 0.006 2 68 28 7 LEU HG H 1.602 0.007 1 69 28 7 LEU HD1 H 0.808 0.004 2 70 28 7 LEU HD2 H 0.898 0.007 2 71 28 7 LEU C C 178.432 0.000 1 72 28 7 LEU CA C 56.844 0.093 1 73 28 7 LEU CB C 41.560 0.083 1 74 28 7 LEU CG C 26.981 0.011 1 75 28 7 LEU CD1 C 23.411 0.044 2 76 28 7 LEU CD2 C 25.009 0.035 2 77 28 7 LEU N N 119.658 0.014 1 78 29 8 LYS H H 7.778 0.004 1 79 29 8 LYS HA H 3.939 0.004 1 80 29 8 LYS HB2 H 1.733 0.004 2 81 29 8 LYS HB3 H 1.661 0.003 2 82 29 8 LYS HG2 H 1.322 0.008 2 83 29 8 LYS HG3 H 1.225 0.004 2 84 29 8 LYS HD2 H 1.598 0.004 1 85 29 8 LYS HD3 H 1.598 0.004 1 86 29 8 LYS HE2 H 2.878 0.005 1 87 29 8 LYS HE3 H 2.878 0.005 1 88 29 8 LYS C C 177.895 0.000 1 89 29 8 LYS CA C 58.327 0.067 1 90 29 8 LYS CB C 32.364 0.038 1 91 29 8 LYS CG C 25.045 0.022 1 92 29 8 LYS CD C 29.305 0.032 1 93 29 8 LYS CE C 42.031 0.047 1 94 29 8 LYS N N 119.090 0.072 1 95 30 9 TRP H H 7.660 0.003 1 96 30 9 TRP HA H 4.353 0.005 1 97 30 9 TRP HB2 H 3.165 0.005 2 98 30 9 TRP HB3 H 3.253 0.006 2 99 30 9 TRP HD1 H 7.193 0.003 1 100 30 9 TRP HE1 H 10.141 0.003 1 101 30 9 TRP HE3 H 7.265 0.020 1 102 30 9 TRP HZ2 H 7.364 0.019 1 103 30 9 TRP HZ3 H 7.144 0.004 1 104 30 9 TRP HH2 H 7.175 0.006 1 105 30 9 TRP C C 175.521 0.000 1 106 30 9 TRP CA C 58.947 0.028 1 107 30 9 TRP CB C 28.729 0.026 1 108 30 9 TRP CD1 C 127.070 0.000 1 109 30 9 TRP CE3 C 120.467 0.000 1 110 30 9 TRP CZ2 C 114.524 0.000 1 111 30 9 TRP CZ3 C 129.464 0.000 1 112 30 9 TRP CH2 C 124.482 0.000 1 113 30 9 TRP N N 120.165 0.009 1 114 30 9 TRP NE1 N 129.806 0.028 1 115 31 10 TRP H H 8.158 0.003 1 116 31 10 TRP HA H 3.962 0.003 1 117 31 10 TRP HB2 H 2.825 0.008 2 118 31 10 TRP HB3 H 2.970 0.004 2 119 31 10 TRP HD1 H 6.974 0.005 1 120 31 10 TRP HE1 H 9.994 0.003 1 121 31 10 TRP HE3 H 6.968 0.004 1 122 31 10 TRP HZ2 H 7.368 0.006 1 123 31 10 TRP HZ3 H 6.964 0.004 1 124 31 10 TRP HH2 H 7.092 0.003 1 125 31 10 TRP C C 176.327 0.000 1 126 31 10 TRP CA C 58.688 0.070 1 127 31 10 TRP CB C 29.109 0.026 1 128 31 10 TRP CD1 C 126.471 0.000 1 129 31 10 TRP CE3 C 121.659 0.000 1 130 31 10 TRP CZ2 C 114.302 0.000 1 131 31 10 TRP CZ3 C 129.418 0.000 1 132 31 10 TRP CH2 C 124.000 0.000 1 133 31 10 TRP N N 121.173 0.015 1 134 31 10 TRP NE1 N 128.831 0.008 1 135 32 11 ARG H H 7.808 0.004 1 136 32 11 ARG HA H 4.086 0.007 1 137 32 11 ARG HB2 H 1.786 0.003 2 138 32 11 ARG HB3 H 1.699 0.004 2 139 32 11 ARG HG2 H 1.536 0.005 2 140 32 11 ARG HG3 H 1.616 0.020 2 141 32 11 ARG HD2 H 3.106 0.004 1 142 32 11 ARG HD3 H 3.106 0.004 1 143 32 11 ARG HE H 7.231 0.000 1 144 32 11 ARG C C 176.629 0.000 1 145 32 11 ARG CA C 56.736 0.074 1 146 32 11 ARG CB C 30.648 0.027 1 147 32 11 ARG CG C 27.410 0.028 1 148 32 11 ARG CD C 43.325 0.038 1 149 32 11 ARG N N 118.781 0.058 1 150 32 11 ARG NE N 108.420 0.000 1 151 33 12 SER H H 7.848 0.003 1 152 33 12 SER HA H 4.249 0.004 1 153 33 12 SER HB2 H 3.835 0.004 2 154 33 12 SER HB3 H 3.891 0.005 2 155 33 12 SER C C 177.148 0.000 1 156 33 12 SER CA C 59.200 0.038 1 157 33 12 SER CB C 63.565 0.081 1 158 33 12 SER N N 115.910 0.019 1 159 34 13 GLU H H 8.208 0.002 1 160 34 13 GLU HA H 4.089 0.005 1 161 34 13 GLU HB2 H 1.628 0.005 2 162 34 13 GLU HB3 H 1.867 0.004 2 163 34 13 GLU HG2 H 1.961 0.004 1 164 34 13 GLU HG3 H 1.961 0.004 1 165 34 13 GLU C C 176.430 0.000 1 166 34 13 GLU CB C 29.969 0.039 1 167 34 13 GLU CG C 35.743 0.135 1 168 34 13 GLU N N 122.201 0.017 1 169 35 14 GLU H H 8.063 0.005 1 170 35 14 GLU HA H 4.079 0.008 1 171 35 14 GLU HB2 H 1.795 0.005 2 172 35 14 GLU HB3 H 1.904 0.006 2 173 35 14 GLU HG2 H 2.166 0.004 1 174 35 14 GLU HG3 H 2.166 0.004 1 175 35 14 GLU C C 176.355 0.000 1 176 35 14 GLU CA C 56.999 0.062 1 177 35 14 GLU CB C 29.989 0.048 1 178 35 14 GLU CG C 36.391 0.039 1 179 35 14 GLU N N 120.042 0.008 1 180 36 15 ALA H H 7.919 0.010 1 181 36 15 ALA HA H 4.180 0.006 1 182 36 15 ALA HB H 1.243 0.007 1 183 36 15 ALA C C 177.583 0.000 1 184 36 15 ALA CA C 52.563 0.034 1 185 36 15 ALA CB C 19.018 0.034 1 186 36 15 ALA N N 123.727 0.055 1 187 37 16 GLN H H 8.078 0.006 1 188 37 16 GLN HA H 4.230 0.004 1 189 37 16 GLN HB2 H 1.927 0.005 2 190 37 16 GLN HB3 H 2.079 0.004 2 191 37 16 GLN HG2 H 2.307 0.004 1 192 37 16 GLN HG3 H 2.308 0.004 1 193 37 16 GLN HE21 H 6.743 0.000 1 194 37 16 GLN HE22 H 7.445 0.000 1 195 37 16 GLN C C 175.634 0.000 1 196 37 16 GLN CA C 55.713 0.052 1 197 37 16 GLN CB C 29.517 0.034 1 198 37 16 GLN CG C 33.697 0.039 1 199 37 16 GLN N N 118.623 0.032 1 200 37 16 GLN NE2 N 112.518 0.002 1 201 38 17 ASP H H 8.284 0.001 1 202 38 17 ASP HA H 4.585 0.005 1 203 38 17 ASP HB2 H 2.612 0.006 2 204 38 17 ASP HB3 H 2.717 0.004 2 205 38 17 ASP C C 176.106 0.000 1 206 38 17 ASP CA C 54.356 0.039 1 207 38 17 ASP CB C 41.116 0.039 1 208 38 17 ASP N N 120.925 0.004 1 209 39 18 MET H H 8.284 0.002 1 210 39 18 MET HA H 4.505 0.004 1 211 39 18 MET HB2 H 1.985 0.003 2 212 39 18 MET HB3 H 2.157 0.003 2 213 39 18 MET HG2 H 2.501 0.005 2 214 39 18 MET HG3 H 2.611 0.005 2 215 39 18 MET HE H 2.060 0.005 1 216 39 18 MET C C 175.834 0.000 1 217 39 18 MET CA C 55.283 0.037 1 218 39 18 MET CB C 32.740 0.052 1 219 39 18 MET CG C 32.305 0.049 1 220 39 18 MET CE C 16.890 0.065 1 221 39 18 MET N N 121.220 0.006 1 222 40 19 GLY H H 8.080 0.001 1 223 40 19 GLY HA2 H 3.737 0.003 1 224 40 19 GLY HA3 H 3.737 0.003 1 225 40 19 GLY CA C 46.218 0.045 1 226 40 19 GLY N N 115.777 0.001 1 stop_ save_