data_19222 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; NMR Studies of DNA Support the Role of Pre-Existing Minor Groove Variations in Nucleosome Indirect Readout ; _BMRB_accession_number 19222 _BMRB_flat_file_name bmr19222.str _Entry_type original _Submission_date 2013-05-03 _Accession_date 2013-05-03 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details "Using NMR to examine the properties of four dodecamers that together cover 39 bp of the 5' half of the strong nucleosome positioning sequence 601." loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Xu Xiaoqian . . 2 'Ben Imeddourene' Akli . . 3 Zargarian Loussine . . 4 Foloppe Nicolas . . 5 Mauffret Olivier . . 6 Hartmann Brigitte . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 24 residual_dipolar_couplings 1 stop_ loop_ _Data_type _Data_type_count "31P chemical shifts" 264 "residual dipolar couplings" 317 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2015-03-30 update author 'update entry citation, and data, etc.' 2013-06-06 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'NMR Studies of DNA Support the Role of Pre-Existing Minor Groove Variations in Nucleosome Indirect Readout' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID ? loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Xu Xiaoqian . . 2 'Ben Imeddourene' Akli . . 3 Zargarian Loussine . . 4 Foloppe Nicolas . . 5 Mauffret Olivier . . 6 Hartmann Brigitte . . stop_ _Journal_abbreviation Biochemistry _Journal_name_full Biochemistry _Journal_volume 53 _Journal_issue 35 _Journal_ISSN 0006-2960 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 5601 _Page_last 5612 _Year 2014 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name "Four dodecamers that together cover 39 bp of the 5' half of the strong nucleosome positioning sequence 601" _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'DNA dodecamer 1-1' $DNA_dodecamer_1-1 'DNA dodecamer 1-2' $DNA_dodecamer_1-2 'DNA dodecamer 2-1' $DNA_dodecamer_2-1 'DNA dodecamer 2-2' $DNA_dodecamer_2-2 'DNA dodecamer 3-1' $DNA_dodecamer_3-1 'DNA dodecamer 3-2' $DNA_dodecamer_3-2 'DNA dodecamer 4-1' $DNA_dodecamer_4-1 'DNA dodecamer 4-2' $DNA_dodecamer_4-2 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_DNA_dodecamer_1-1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class DNA _Name_common DNA_dodecamer_1-1 _Molecular_mass . _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 12 _Mol_residue_sequence TCGTAGCAAGCT loop_ _Residue_seq_code _Residue_label 1 DT 2 DC 3 DG 4 DT 5 DA 6 DG 7 DC 8 DA 9 DA 10 DG 11 DC 12 DT stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ save_DNA_dodecamer_1-2 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class DNA _Name_common DNA_dodecamer_1-2 _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 12 _Mol_residue_sequence AGCTTGCTACGA loop_ _Residue_seq_code _Residue_label 1 DA 2 DG 3 DC 4 DT 5 DT 6 DG 7 DC 8 DT 9 DA 10 DC 11 DG 12 DA stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ save_DNA_dodecamer_2-1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class DNA _Name_common DNA_dodecamer_2-1 _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 12 _Mol_residue_sequence GCTCTAGCACCG loop_ _Residue_seq_code _Residue_label 1 DG 2 DC 3 DT 4 DC 5 DT 6 DA 7 DG 8 DC 9 DA 10 DC 11 DC 12 DG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ save_DNA_dodecamer_2-2 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class DNA _Name_common DNA_dodecamer_2-2 _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 12 _Mol_residue_sequence CGGTGCTAGAGC loop_ _Residue_seq_code _Residue_label 1 DC 2 DG 3 DG 4 DT 5 DG 6 DC 7 DT 8 DA 9 DG 10 DA 11 DG 12 DC stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ save_DNA_dodecamer_3-1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class DNA _Name_common DNA_dodecamer_3-1 _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 12 _Mol_residue_sequence CCGCTTAAACGC loop_ _Residue_seq_code _Residue_label 1 DC 2 DC 3 DG 4 DC 5 DT 6 DT 7 DA 8 DA 9 DA 10 DC 11 DG 12 DC stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ save_DNA_dodecamer_3-2 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class DNA _Name_common DNA_dodecamer_3-2 _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 12 _Mol_residue_sequence GCGTTTAAGCGG loop_ _Residue_seq_code _Residue_label 1 DG 2 DC 3 DG 4 DT 5 DT 6 DT 7 DA 8 DA 9 DG 10 DC 11 DG 12 DG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ save_DNA_dodecamer_4-1 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class DNA _Name_common DNA_dodecamer_4-1 _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 12 _Mol_residue_sequence CGCACGTACGCG loop_ _Residue_seq_code _Residue_label 1 DC 2 DG 3 DC 4 DA 5 DC 6 DG 7 DT 8 DA 9 DC 10 DG 11 DC 12 DG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ save_DNA_dodecamer_4-2 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class DNA _Name_common DNA_dodecamer_4-2 _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 12 _Mol_residue_sequence CGCGTACGTGCG loop_ _Residue_seq_code _Residue_label 1 DC 2 DG 3 DC 4 DG 5 DT 6 DA 7 DC 8 DG 9 DT 10 DG 11 DC 12 DG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species _Details $DNA_dodecamer_1-1 . . . . . . "Four dodecamers that together cover 39 bp of the 5' half of the strong nucleosome positioning sequence 601" $DNA_dodecamer_1-2 . . . . . . "Four dodecamers that together cover 39 bp of the 5' half of the strong nucleosome positioning sequence 601" $DNA_dodecamer_2-1 . . . . . . "Four dodecamers that together cover 39 bp of the 5' half of the strong nucleosome positioning sequence 601" $DNA_dodecamer_2-2 . . . . . . "Four dodecamers that together cover 39 bp of the 5' half of the strong nucleosome positioning sequence 601" $DNA_dodecamer_3-1 . . . . . . "Four dodecamers that together cover 39 bp of the 5' half of the strong nucleosome positioning sequence 601" $DNA_dodecamer_3-2 . . . . . . "Four dodecamers that together cover 39 bp of the 5' half of the strong nucleosome positioning sequence 601" $DNA_dodecamer_4-1 . . . . . . "Four dodecamers that together cover 39 bp of the 5' half of the strong nucleosome positioning sequence 601" $DNA_dodecamer_4-2 . . . . . . "Four dodecamers that together cover 39 bp of the 5' half of the strong nucleosome positioning sequence 601" stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $DNA_dodecamer_1-1 'chemical synthesis' . . . . . $DNA_dodecamer_1-2 'chemical synthesis' . . . . . $DNA_dodecamer_2-1 'chemical synthesis' . . . . . $DNA_dodecamer_2-2 'chemical synthesis' . . . . . $DNA_dodecamer_3-1 'chemical synthesis' . . . . . $DNA_dodecamer_3-2 'chemical synthesis' . . . . . $DNA_dodecamer_4-1 'chemical synthesis' . . . . . $DNA_dodecamer_4-2 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details ; DNA Dodecamers Sequences: 5'-TCGTAGCAAGCT-3' 5'-GCTCTAGCACCG-3' 5'-CCGCTTAAACGC-3' 5'-CGCACGTACGCG-3' ; loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $DNA_dodecamer_1-1 1.5 mM 'natural abundance' $DNA_dodecamer_1-2 1.5 mM 'natural abundance' $DNA_dodecamer_2-1 1.5 mM 'natural abundance' $DNA_dodecamer_2-2 1.5 mM 'natural abundance' $DNA_dodecamer_3-1 1.5 mM 'natural abundance' $DNA_dodecamer_3-2 1.5 mM 'natural abundance' $DNA_dodecamer_4-1 1.5 mM 'natural abundance' $DNA_dodecamer_4-2 1.5 mM 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address 'T. D. Goddard and D. G. Kneller, SPARKY 3, University of California, San Francisco' . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_NMRPIPE _Saveframe_category software _Name NMRPIPE _Version 8.2 loop_ _Vendor _Address _Electronic_address 'F. Delaglio, S. Grzesiek, G. W. Vuister, G. Zhu, J. Pfeifer and A. Bax: NMRPipe: a multidimensional spectral processing system based on UNIX pipes. J. Biomol. NMR. 6, 277-293 (1995).' . . stop_ loop_ _Task processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ save_2D_1H-1H_TOCSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_1 save_ save_2D_1H-13C_TROSY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C TROSY' _Sample_label $sample_1 save_ save_2D_1H-13C_HSQC_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_1 save_ save_2D_1H-31P_Hetero_-_Correlation_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-31P Hetero - Correlation' _Sample_label $sample_1 save_ save_NMR_spectrometer_expt _Saveframe_category NMR_applied_experiment _Experiment_name . _BMRB_pulse_sequence_accession_number . _Details . save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.1 . M pH 6.5 . pH pressure 1 . atm temperature 293 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.1 . M pH 6.5 . pH pressure 1 . atm temperature 303 . K stop_ save_ save_sample_conditions_3 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.1 . M pH 6.5 . pH pressure 1 . atm temperature 313 . K stop_ save_ save_sample_conditions_4 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.1 . M pH 6.5 . pH pressure 1 . atm temperature 298 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio TSP C 13 'methyl carbon' ppm 0 internal direct . . . 1.0 TSP H 1 'methyl protons' ppm 0 internal direct . . . 1.0 TMP P 31 phosphorus ppm 0 internal direct . . . 1.0 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 1-1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 DC P P -4.24 0.02 . 2 3 3 DG P P -3.80 0.02 . 3 4 4 DT P P -4.45 0.02 . 4 5 5 DA P P -4.29 0.02 . 5 6 6 DG P P -4.20 0.02 . 6 7 7 DC P P -4.17 0.02 . 7 8 8 DA P P -4.24 0.02 . 8 9 9 DA P P -4.27 0.02 . 9 10 10 DG P P -4.20 0.02 . 10 11 11 DC P P -4.07 0.02 . 11 12 12 DT P P -4.18 0.02 . stop_ save_ save_assigned_chem_shift_list_1_2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 1-2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 14 2 DG P P -4.20 0.02 . 2 15 3 DC P P -4.18 0.02 . 3 16 4 DT P P -4.39 0.02 . 4 17 5 DT P P -4.46 0.02 . 5 18 6 DG P P -4.17 0.02 . 6 19 7 DC P P -4.05 0.02 . 7 20 8 DT P P -4.50 0.02 . 8 21 9 DA P P -4.18 0.02 . 9 22 10 DC P P -4.20 0.02 . 10 23 11 DG P P -4.06 0.02 . 11 24 12 DA P P -4.11 0.02 . stop_ save_ save_assigned_chem_shift_list_1_3 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 2-1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 DC P P -4.19 0.02 . 2 3 3 DT P P -4.45 0.02 . 3 4 4 DC P P -4.25 0.02 . 4 5 5 DT P P -4.37 0.02 . 5 6 6 DA P P -4.25 0.02 . 6 7 7 DG P P -4.27 0.02 . 7 8 8 DC P P -4.1 0.02 . 8 9 9 DA P P -4.25 0.02 . 9 10 10 DC P P -4.33 0.02 . 10 11 11 DC P P -4.12 0.02 . 11 12 12 DG P P -3.83 0.02 . stop_ save_ save_assigned_chem_shift_list_1_4 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 2-2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 14 2 DG P P -4.07 0.02 . 2 15 3 DG P P -3.85 0.02 . 3 16 4 DT P P -4.45 0.02 . 4 17 5 DG P P -4.31 0.02 . 5 18 6 DC P P -4.09 0.02 . 6 19 7 DT P P -4.5 0.02 . 7 20 8 DA P P -4.25 0.02 . 8 21 9 DG P P -4.24 0.02 . 9 22 10 DA P P -4.02 0.02 . 10 23 11 DG P P -4.24 0.02 . 11 24 12 DC P P -4.05 0.02 . stop_ save_ save_assigned_chem_shift_list_1_5 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 3-1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 DC P P -4.11 0.02 . 2 3 3 DG P P -3.92 0.02 . 3 4 4 DC P P -4.12 0.02 . 4 5 5 DT P P -4.35 0.02 . 5 6 6 DT P P -4.46 0.02 . 6 7 7 DA P P -4.23 0.02 . 7 8 8 DA P P -4.34 0.02 . 8 9 9 DA P P -4.18 0.02 . 9 10 10 DC P P -4.27 0.02 . 10 11 11 DG P P -4.22 0.02 . 11 12 12 DC P P -4.07 0.02 . stop_ save_ save_assigned_chem_shift_list_1_6 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 3-2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 14 2 DC P P -4.29 0.02 . 2 15 3 DG P P -3.87 0.02 . 3 16 4 DT P P -4.41 0.02 . 4 17 5 DT P P -4.51 0.02 . 5 18 6 DT P P -4.41 0.02 . 6 19 7 DA P P -4.29 0.02 . 7 20 8 DA P P -4.18 0.02 . 8 21 9 DG P P -4.19 0.02 . 9 22 10 DC P P -4.17 0.02 . 10 23 11 DG P P -4.12 0.02 . 11 24 12 DG P P -3.93 0.02 . stop_ save_ save_assigned_chem_shift_list_1_7 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 4-1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 DG P P -4.14 0.02 . 2 3 3 DC P P -4.25 0.02 . 3 4 4 DA P P -4.03 0.02 . 4 5 5 DC P P -4.33 0.02 . 5 6 6 DG P P -4.01 0.02 . 6 7 7 DT P P -4.47 0.02 . 7 8 8 DA P P -4.26 0.02 . 8 9 9 DC P P -4.26 0.02 . 9 10 10 DG P P -4.09 0.02 . 10 11 11 DC P P -4.17 0.02 . 11 12 12 DG P P -3.98 0.02 . stop_ save_ save_assigned_chem_shift_list_1_8 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 4-2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 14 2 DG P P -4.14 0.02 . 2 15 3 DC P P -4.25 0.02 . 3 16 4 DG P P -3.99 0.02 . 4 17 5 DT P P -4.43 0.02 . 5 18 6 DA P P -4.26 0.02 . 6 19 7 DC P P -4.26 0.02 . 7 20 8 DG P P -4.09 0.02 . 8 21 9 DT P P -4.47 0.02 . 9 22 10 DG P P -4.26 0.02 . 10 23 11 DC P P -4.04 0.02 . 11 24 12 DG P P -3.87 0.02 . stop_ save_ save_assigned_chem_shift_list_2_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_2 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 1-1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 DC P P -4.17 0.02 . 2 3 3 DG P P -3.78 0.02 . 3 4 4 DT P P -4.42 0.02 . 4 5 5 DA P P -4.27 0.02 . 5 6 6 DG P P -4.16 0.02 . 6 7 7 DC P P -4.15 0.02 . 7 8 8 DA P P -4.22 0.02 . 8 9 9 DA P P -4.24 0.02 . 9 10 10 DG P P -4.16 0.02 . 10 11 11 DC P P -4.04 0.02 . 11 12 12 DT P P -4.11 0.02 . stop_ save_ save_assigned_chem_shift_list_2_2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_2 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 1-2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 14 2 DG P P -4.15 0.02 . 2 15 3 DC P P -4.16 0.02 . 3 16 4 DT P P -4.35 0.02 . 4 17 5 DT P P -4.42 0.02 . 5 18 6 DG P P -4.15 0.02 . 6 19 7 DC P P -4.02 0.02 . 7 20 8 DT P P -4.46 0.02 . 8 21 9 DA P P -4.16 0.02 . 9 22 10 DC P P -4.16 0.02 . 10 23 11 DG P P -4.04 0.02 . 11 24 12 DA P P -4.07 0.02 . stop_ save_ save_assigned_chem_shift_list_2_3 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_2 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 2-1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 DC P P -4.25 0.02 . 2 3 3 DT P P -4.41 0.02 . 3 4 4 DC P P -4.22 0.02 . 4 5 5 DT P P -4.34 0.02 . 5 6 6 DA P P -4.23 0.02 . 6 7 7 DG P P -4.24 0.02 . 7 8 8 DC P P -4.07 0.02 . 8 9 9 DA P P -4.23 0.02 . 9 10 10 DC P P -4.3 0.02 . 10 11 11 DC P P -4.09 0.02 . 11 12 12 DG P P -3.91 0.02 . stop_ save_ save_assigned_chem_shift_list_2_4 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_2 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 2-2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 14 2 DG P P -4.11 0.02 . 2 15 3 DG P P -3.85 0.02 . 3 16 4 DT P P -4.42 0.02 . 4 17 5 DG P P -4.29 0.02 . 5 18 6 DC P P -4.09 0.02 . 6 19 7 DT P P -4.46 0.02 . 7 20 8 DA P P -4.24 0.02 . 8 21 9 DG P P -4.23 0.02 . 9 22 10 DA P P -3.99 0.02 . 10 23 11 DG P P -4.23 0.02 . 11 24 12 DC P P -4.15 0.02 . stop_ save_ save_assigned_chem_shift_list_2_5 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_2 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 3-1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 DC P P -4.08 0.02 . 2 3 3 DG P P -3.91 0.02 . 3 4 4 DC P P -4.11 0.02 . 4 5 5 DT P P -4.33 0.02 . 5 6 6 DT P P -4.43 0.02 . 6 7 7 DA P P -4.23 0.02 . 7 8 8 DA P P -4.29 0.02 . 8 9 9 DA P P -4.17 0.02 . 9 10 10 DC P P -4.23 0.02 . 10 11 11 DG P P -4.18 0.02 . 11 12 12 DC P P -4.05 0.02 . stop_ save_ save_assigned_chem_shift_list_2_6 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_2 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 3-2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 14 2 DC P P -4.25 0.02 . 2 15 3 DG P P -3.91 0.02 . 3 16 4 DT P P -4.38 0.02 . 4 17 5 DT P P -4.47 0.02 . 5 18 6 DT P P -4.38 0.02 . 6 19 7 DA P P -4.26 0.02 . 7 20 8 DA P P -4.17 0.02 . 8 21 9 DG P P -4.17 0.02 . 9 22 10 DC P P -4.15 0.02 . 10 23 11 DG P P -4.11 0.02 . 11 24 12 DG P P -3.92 0.02 . stop_ save_ save_assigned_chem_shift_list_2_7 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_2 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 4-1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 DG P P -4.1 0.02 . 2 3 3 DC P P -4.23 0.02 . 3 4 4 DA P P -4.03 0.02 . 4 5 5 DC P P -4.31 0.02 . 5 6 6 DG P P -3.99 0.02 . 6 7 7 DT P P -4.44 0.02 . 7 8 8 DA P P -4.24 0.02 . 8 9 9 DC P P -4.24 0.02 . 9 10 10 DG P P -4.07 0.02 . 10 11 11 DC P P -4.14 0.02 . 11 12 12 DG P P -3.96 0.02 . stop_ save_ save_assigned_chem_shift_list_2_8 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_2 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 4-2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 14 2 DG P P -4.1 0.02 . 2 15 3 DC P P -4.23 0.02 . 3 16 4 DG P P -3.98 0.02 . 4 17 5 DT P P -4.4 0.02 . 5 18 6 DA P P -4.24 0.02 . 6 19 7 DC P P -4.24 0.02 . 7 20 8 DG P P -4.07 0.02 . 8 21 9 DT P P -4.44 0.02 . 9 22 10 DG P P -4.24 0.02 . 10 23 11 DC P P -4.02 0.02 . 11 24 12 DG P P -3.91 0.02 . stop_ save_ save_assigned_chem_shift_list_3_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_3 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 1-1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 DC P P -4.11 0.02 . 2 3 3 DG P P -3.80 0.02 . 3 4 4 DT P P -4.38 0.02 . 4 5 5 DA P P -4.24 0.02 . 5 6 6 DG P P -4.13 0.02 . 6 7 7 DC P P -4.16 0.02 . 7 8 8 DA P P -4.21 0.02 . 8 9 9 DA P P -4.22 0.02 . 9 10 10 DG P P -4.13 0.02 . 10 11 11 DC P P -4.02 0.02 . 11 12 12 DT P P -4.06 0.02 . stop_ save_ save_assigned_chem_shift_list_3_2 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_3 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 1-2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 14 2 DG P P -4.12 0.02 . 2 15 3 DC P P -4.16 0.02 . 3 16 4 DT P P -4.32 0.02 . 4 17 5 DT P P -4.39 0.02 . 5 18 6 DG P P -4.16 0.02 . 6 19 7 DC P P -4.03 0.02 . 7 20 8 DT P P -4.42 0.02 . 8 21 9 DA P P -4.16 0.02 . 9 22 10 DC P P -4.13 0.02 . 10 23 11 DG P P -4.03 0.02 . 11 24 12 DA P P -4.04 0.02 . stop_ save_ save_assigned_chem_shift_list_3_3 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_3 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 2-1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 DC P P -4.21 0.02 . 2 3 3 DT P P -4.38 0.02 . 3 4 4 DC P P -4.21 0.02 . 4 5 5 DT P P -4.32 0.02 . 5 6 6 DA P P -4.23 0.02 . 6 7 7 DG P P -4.23 0.02 . 7 8 8 DC P P -4.07 0.02 . 8 9 9 DA P P -4.23 0.02 . 9 10 10 DC P P -4.29 0.02 . 10 11 11 DC P P -4.08 0.02 . 11 12 12 DG P P -3.88 0.02 . stop_ save_ save_assigned_chem_shift_list_3_4 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_3 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 2-2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 14 2 DG P P -4.09 0.02 . 2 15 3 DG P P -3.87 0.02 . 3 16 4 DT P P -4.49 0.02 . 4 17 5 DG P P -4.27 0.02 . 5 18 6 DC P P -4.09 0.02 . 6 19 7 DT P P -4.43 0.02 . 7 20 8 DA P P -4.23 0.02 . 8 21 9 DG P P -4.22 0.02 . 9 22 10 DA P P -3.99 0.02 . 10 23 11 DG P P -4.22 0.02 . 11 24 12 DC P P -4.16 0.02 . stop_ save_ save_assigned_chem_shift_list_3_5 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_3 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 3-1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 DC P P -4.04 0.02 . 2 3 3 DG P P -3.9 0.02 . 3 4 4 DC P P -4.09 0.02 . 4 5 5 DT P P -4.3 0.02 . 5 6 6 DT P P -4.39 0.02 . 6 7 7 DA P P -4.21 0.02 . 7 8 8 DA P P -4.22 0.02 . 8 9 9 DA P P -4.16 0.02 . 9 10 10 DC P P -4.2 0.02 . 10 11 11 DG P P -4.15 0.02 . 11 12 12 DC P P -4.03 0.02 . stop_ save_ save_assigned_chem_shift_list_3_6 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_3 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 3-2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 14 2 DC P P -4.21 0.02 . 2 15 3 DG P P -3.9 0.02 . 3 16 4 DT P P -4.34 0.02 . 4 17 5 DT P P -4.42 0.02 . 5 18 6 DT P P -4.34 0.02 . 6 19 7 DA P P -4.23 0.02 . 7 20 8 DA P P -4.16 0.02 . 8 21 9 DG P P -4.15 0.02 . 9 22 10 DC P P -4.12 0.02 . 10 23 11 DG P P -4.09 0.02 . 11 24 12 DG P P -3.91 0.02 . stop_ save_ save_assigned_chem_shift_list_3_7 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_3 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 4-1' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 DG P P -4.05 0.02 . 2 3 3 DC P P -4.21 0.02 . 3 4 4 DA P P -4.02 0.02 . 4 5 5 DC P P -4.27 0.02 . 5 6 6 DG P P -3.98 0.02 . 6 7 7 DT P P -4.4 0.02 . 7 8 8 DA P P -4.22 0.02 . 8 9 9 DC P P -4.21 0.02 . 9 10 10 DG P P -4.05 0.02 . 10 11 11 DC P P -4.11 0.02 . 11 12 12 DG P P -3.95 0.02 . stop_ save_ save_assigned_chem_shift_list_3_8 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-31P Hetero - Correlation' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_3 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'DNA dodecamer 4-2' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 14 2 DG P P -4.05 0.02 . 2 15 3 DC P P -4.21 0.02 . 3 16 4 DG P P -3.98 0.02 . 4 17 5 DT P P -4.36 0.02 . 5 18 6 DA P P -4.22 0.02 . 6 19 7 DC P P -4.21 0.02 . 7 20 8 DG P P -4.04 0.02 . 8 21 9 DT P P -4.4 0.02 . 9 22 10 DG P P -4.22 0.02 . 10 23 11 DC P P -4.00 0.02 . 11 24 12 DG P P -3.9 0.02 . stop_ save_ save_RDC_list_1 _Saveframe_category residual_dipolar_couplings loop_ _Sample_label $sample_1 stop_ _Details 'residual dipolar coupling error : 2 Hz' _Sample_conditions_label $sample_conditions_4 _Spectrometer_frequency_1H 500 _Text_data_format . _Text_data . loop_ _Residual_dipolar_coupling_ID _Atom_one_residue_seq_code _Atom_one_residue_label _Atom_one_atom_name _Atom_two_residue_seq_code _Atom_two_residue_label _Atom_two_atom_name _Residual_dipolar_coupling_value _Atom_one_mol_system_component_name _Atom_two_mol_system_component_name _Residual_dipolar_coupling_min_value _Residual_dipolar_coupling_max_value _Residual_dipolar_coupling_value_error DCH 1 DT C6 1 DT H6 12 ? ? . . 2 DCH 1 DT C7 1 DT H71 11 ? ? . . 2 DCH 2 DC C6 2 DC H6 14 ? ? . . 2 DCH 2 DC C5 2 DC H5 9 ? ? . . 2 DCH 3 DG C8 3 DG H8 10 ? ? . . 2 DCH 4 DT C6 4 DT H6 12 ? ? . . 2 DCH 5 DA C8 5 DA H8 11 ? ? . . 2 DCH 5 DA C2 5 DA H2 10 ? ? . . 2 DCH 6 DG C8 6 DG H8 10 ? ? . . 2 DCH 7 DC C6 7 DC H6 7 ? ? . . 2 DCH 7 DC C6 7 DC H6 7 ? ? . . 2 DCH 8 DA C8 8 DA H8 7 ? ? . . 2 DCH 8 DA C2 8 DA H2 7 ? ? . . 2 DCH 9 DA C8 9 DA H8 7 ? ? . . 2 DCH 9 DA C2 9 DA H2 7 ? ? . . 2 DCH 10 DG C8 10 DG H8 4 ? ? . . 2 DCH 11 DC C6 11 DC H6 1 ? ? . . 2 DCH 12 DT C7 12 DT H71 7 ? ? . . 2 DCH 1 DA C8 1 DA H8 5 ? ? . . 2 DCH 2 DG C8 2 DG H8 10 ? ? . . 2 DCH 3 DC C6 3 DC H6 11 ? ? . . 2 DCH 3 DC C6 3 DC H6 10 ? ? . . 2 DCH 4 DT C6 4 DT H6 13 ? ? . . 2 DCH 4 DT C7 4 DT H71 4 ? ? . . 2 DCH 5 DT C6 5 DT H6 17 ? ? . . 2 DCH 5 DT C7 5 DT H71 16 ? ? . . 2 DCH 7 DC C6 7 DC H6 9 ? ? . . 2 DCH 7 DC C6 7 DC H6 9 ? ? . . 2 DCH 8 DT C6 8 DT H6 11 ? ? . . 2 DCH 9 DA C8 9 DA H8 7 ? ? . . 2 DCH 9 DA C2 9 DA H2 9 ? ? . . 2 DCH 10 DC C6 10 DC H6 8 ? ? . . 2 DCH 10 DC C6 10 DC H6 9 ? ? . . 2 DCH 11 DG C8 11 DG H8 4 ? ? . . 2 DCH 12 DA C2 12 DA H2 8 ? ? . . 2 DCH 1 DT C1' 1 DT H1' 6 ? ? . . 2 DCH 2 DC C1' 2 DC H1' 6 ? ? . . 2 DCH 3 DG C1' 3 DG H1' 6 ? ? . . 2 DCH 4 DT C1' 4 DT H1' 6 ? ? . . 2 DCH 5 DA C1' 5 DA H1' 13 ? ? . . 2 DCH 6 DG C1' 6 DG H1' 7 ? ? . . 2 DCH 7 DC C1' 7 DC H1' 10 ? ? . . 2 DCH 8 DA C1' 8 DA H1' -1 ? ? . . 2 DCH 9 DA C1' 9 DA H1' -1 ? ? . . 2 DCH 10 DG C1' 10 DG H1' 9 ? ? . . 2 DCH 11 DC C1' 11 DC H1' 0 ? ? . . 2 DCH 12 DT C1' 12 DT H1' 3 ? ? . . 2 DCH 1 DA C1' 1 DA H1' 1 ? ? . . 2 DCH 2 DG C1' 2 DG H1' 12 ? ? . . 2 DCH 3 DC C1' 3 DC H1' 2 ? ? . . 2 DCH 4 DT C1' 4 DT H1' 7 ? ? . . 2 DCH 5 DT C1' 5 DT H1' 2 ? ? . . 2 DCH 6 DG C1' 6 DG H1' 7 ? ? . . 2 DCH 7 DC C1' 7 DC H1' 12 ? ? . . 2 DCH 8 DT C1' 8 DT H1' 1 ? ? . . 2 DCH 9 DA C1' 9 DA H1' 6 ? ? . . 2 DCH 10 DC C1' 10 DC H1' 11 ? ? . . 2 DCH 11 DG C1' 11 DG H1' 4 ? ? . . 2 DCH 12 DA C1' 12 DA H1' 4 ? ? . . 2 DCH 1 DT C3' 1 DT H3' 5 ? ? . . 2 DCH 2 DC C3' 2 DC H3' -3 ? ? . . 2 DCH 3 DG C3' 3 DG H3' 10 ? ? . . 2 DCH 4 DT C3' 4 DT H3' 1 ? ? . . 2 DCH 5 DA C3' 5 DA H3' -1 ? ? . . 2 DCH 6 DG C3' 6 DG H3' -6 ? ? . . 2 DCH 7 DC C3' 7 DC H3' 0 ? ? . . 2 DCH 9 DA C3' 9 DA H3' 12 ? ? . . 2 DCH 10 DG C3' 10 DG H3' 9 ? ? . . 2 DCH 2 DG C3' 2 DG H3' -5 ? ? . . 2 DCH 3 DC C3' 3 DC H3' 3 ? ? . . 2 DCH 4 DT C3' 4 DT H3' 0 ? ? . . 2 DCH 5 DT C3' 5 DT H3' 5 ? ? . . 2 DCH 6 DG C3' 6 DG H3' 10 ? ? . . 2 DCH 7 DC C3' 7 DC H3' 18 ? ? . . 2 DCH 8 DT C3' 8 DT H3' 8 ? ? . . 2 DCH 10 DC C3' 10 DC H3' 0 ? ? . . 2 DCH 11 DG C3' 11 DG H3' 12 ? ? . . 2 DCH 1 DT C4' 1 DT H4' 10 ? ? . . 2 DCH 2 DC C4' 2 DC H4' 8 ? ? . . 2 DCH 3 DG C4' 3 DG H4' -2 ? ? . . 2 DCH 5 DA C4' 5 DA H4' 4 ? ? . . 2 DCH 7 DC C4' 7 DC H4' 8 ? ? . . 2 DCH 8 DA C4' 8 DA H4' 7 ? ? . . 2 DCH 9 DA C4' 9 DA H4' 16 ? ? . . 2 DCH 10 DG C4' 10 DG H4' 12 ? ? . . 2 DCH 11 DC C4' 11 DC H4' 6 ? ? . . 2 DCH 12 DT C4' 12 DT H4' 4 ? ? . . 2 DCH 1 DA C4' 1 DA H4' 12 ? ? . . 2 DCH 2 DG C4' 2 DG H4' 12 ? ? . . 2 DCH 3 DC C4' 3 DC H4' 12 ? ? . . 2 DCH 5 DT C4' 5 DT H4' 2 ? ? . . 2 DCH 6 DG C4' 6 DG H4' 4 ? ? . . 2 DCH 7 DC C4' 7 DC H4' 13 ? ? . . 2 DCH 9 DA C4' 9 DA H4' -3 ? ? . . 2 DCH 11 DG C4' 11 DG H4' 10 ? ? . . 2 DCH 12 DA C4' 12 DA H4' 8 ? ? . . 2 DCH 1 DG C8 1 DG H8 12 ? ? . . 2 DCH 2 DC C6 2 DC H6 8 ? ? . . 2 DCH 2 DC C5 2 DC H5 6 ? ? . . 2 DCH 3 DT C6 3 DT H6 9 ? ? . . 2 DCH 3 DT C7 3 DT H71 9 ? ? . . 2 DCH 4 DC C6 4 DC H6 11 ? ? . . 2 DCH 4 DC C5 4 DC H5 6 ? ? . . 2 DCH 5 DT C6 5 DT H6 8 ? ? . . 2 DCH 6 DA C8 6 DA H8 18 ? ? . . 2 DCH 6 DA C2 6 DA H2 16 ? ? . . 2 DCH 7 DG C8 7 DG H8 10 ? ? . . 2 DCH 8 DC C6 8 DC H6 7 ? ? . . 2 DCH 9 DA C8 9 DA H8 13 ? ? . . 2 DCH 9 DA C2 9 DA H2 16 ? ? . . 2 DCH 10 DC C6 10 DC H6 9 ? ? . . 2 DCH 10 DC C5 10 DC H5 9 ? ? . . 2 DCH 11 DC C6 11 DC H6 3 ? ? . . 2 DCH 11 DC C5 11 DC H5 2 ? ? . . 2 DCH 12 DG C8 12 DG H8 7 ? ? . . 2 DCH 1 DC C6 1 DC H6 7 ? ? . . 2 DCH 2 DG C8 2 DG H8 12 ? ? . . 2 DCH 3 DG C8 3 DG H8 2 ? ? . . 2 DCH 4 DT C6 4 DT H6 0 ? ? . . 2 DCH 4 DT C7 4 DT H71 2 ? ? . . 2 DCH 5 DG C8 5 DG H8 5 ? ? . . 2 DCH 6 DC C6 6 DC H6 0 ? ? . . 2 DCH 6 DC C5 6 DC H5 1 ? ? . . 2 DCH 7 DT C6 7 DT H6 1 ? ? . . 2 DCH 8 DA C8 8 DA H8 3 ? ? . . 2 DCH 8 DA C2 8 DA H2 4 ? ? . . 2 DCH 9 DG C8 9 DG H8 -6 ? ? . . 2 DCH 10 DA C8 10 DA H8 -5 ? ? . . 2 DCH 10 DA C2 10 DA H2 -1 ? ? . . 2 DCH 11 DG C8 11 DG H8 0 ? ? . . 2 DCH 12 DC C6 12 DC H6 3 ? ? . . 2 DCH 12 DC C5 12 DC H5 1 ? ? . . 2 DCH 1 DG C1' 1 DG H1' 0 ? ? . . 2 DCH 2 DC C1' 2 DC H1' 4 ? ? . . 2 DCH 3 DT C1' 3 DT H1' 6 ? ? . . 2 DCH 4 DC C1' 4 DC H1' 3 ? ? . . 2 DCH 5 DT C1' 5 DT H1' 7 ? ? . . 2 DCH 6 DA C1' 6 DA H1' 4 ? ? . . 2 DCH 7 DG C1' 7 DG H1' -7 ? ? . . 2 DCH 8 DC C1' 8 DC H1' 4 ? ? . . 2 DCH 9 DA C1' 9 DA H1' 0 ? ? . . 2 DCH 10 DC C1' 10 DC H1' 13 ? ? . . 2 DCH 11 DC C1' 11 DC H1' 6 ? ? . . 2 DCH 12 DG C1' 12 DG H1' 6 ? ? . . 2 DCH 1 DC C1' 1 DC H1' 4 ? ? . . 2 DCH 2 DG C1' 2 DG H1' 5 ? ? . . 2 DCH 3 DG C1' 3 DG H1' 4 ? ? . . 2 DCH 4 DT C1' 4 DT H1' 7 ? ? . . 2 DCH 5 DG C1' 5 DG H1' 4 ? ? . . 2 DCH 6 DC C1' 6 DC H1' 2 ? ? . . 2 DCH 7 DT C1' 7 DT H1' 4 ? ? . . 2 DCH 8 DA C1' 8 DA H1' 5 ? ? . . 2 DCH 9 DG C1' 9 DG H1' 14 ? ? . . 2 DCH 10 DA C1' 10 DA H1' 8 ? ? . . 2 DCH 11 DG C1' 11 DG H1' 5 ? ? . . 2 DCH 12 DC C1' 12 DC H1' 5 ? ? . . 2 DCH 2 DC C3' 2 DC H3' -2 ? ? . . 2 DCH 3 DT C3' 3 DT H3' 3 ? ? . . 2 DCH 4 DC C3' 4 DC H3' -2 ? ? . . 2 DCH 5 DT C3' 5 DT H3' -10 ? ? . . 2 DCH 7 DG C3' 7 DG H3' 3 ? ? . . 2 DCH 8 DC C3' 8 DC H3' 3 ? ? . . 2 DCH 9 DA C3' 9 DA H3' -1 ? ? . . 2 DCH 10 DC C3' 10 DC H3' -3 ? ? . . 2 DCH 2 DG C3' 2 DG H3' 2 ? ? . . 2 DCH 3 DG C3' 3 DG H3' 8 ? ? . . 2 DCH 4 DT C3' 4 DT H3' 6 ? ? . . 2 DCH 5 DG C3' 5 DG H3' 11 ? ? . . 2 DCH 6 DC C3' 6 DC H3' 10 ? ? . . 2 DCH 8 DA C3' 8 DA H3' 10 ? ? . . 2 DCH 9 DG C3' 9 DG H3' 11 ? ? . . 2 DCH 1 DG C4' 1 DG H4' 10 ? ? . . 2 DCH 2 DC C4' 2 DC H4' 6 ? ? . . 2 DCH 4 DC C4' 4 DC H4' 4 ? ? . . 2 DCH 5 DT C4' 5 DT H4' 1 ? ? . . 2 DCH 6 DA C4' 6 DA H4' -1 ? ? . . 2 DCH 7 DG C4' 7 DG H4' 5 ? ? . . 2 DCH 8 DC C4' 8 DC H4' -1 ? ? . . 2 DCH 9 DA C4' 9 DA H4' -4 ? ? . . 2 DCH 12 DG C4' 12 DG H4' 8 ? ? . . 2 DCH 3 DG C4' 3 DG H4' 2 ? ? . . 2 DCH 4 DT C4' 4 DT H4' 5 ? ? . . 2 DCH 5 DG C4' 5 DG H4' 3 ? ? . . 2 DCH 6 DC C4' 6 DC H4' 8 ? ? . . 2 DCH 7 DT C4' 7 DT H4' 14 ? ? . . 2 DCH 10 DA C4' 10 DA H4' 3 ? ? . . 2 DCH 11 DG C4' 11 DG H4' -2 ? ? . . 2 DCH 1 DC C6 1 DC H6 9 ? ? . . 2 DCH 1 DC C5 1 DC H5 11 ? ? . . 2 DCH 2 DC C6 2 DC H6 14 ? ? . . 2 DCH 2 DC C5 2 DC H5 15 ? ? . . 2 DCH 3 DG C8 3 DG H8 6 ? ? . . 2 DCH 4 DC C6 4 DC H6 -1 ? ? . . 2 DCH 5 DT C6 5 DT H6 3 ? ? . . 2 DCH 5 DT C7 5 DT H6 3 ? ? . . 2 DCH 6 DT C6 6 DT H6 8 ? ? . . 2 DCH 7 DA C8 7 DA H8 12 ? ? . . 2 DCH 7 DA C2 7 DA H2 12 ? ? . . 2 DCH 8 DA C8 8 DA H8 6 ? ? . . 2 DCH 8 DA C2 8 DA H2 8 ? ? . . 2 DCH 9 DA C8 9 DA H8 4 ? ? . . 2 DCH 9 DA C2 9 DA H2 3 ? ? . . 2 DCH 10 DC C6 10 DC H6 -3 ? ? . . 2 DCH 11 DG C8 11 DG H8 -3 ? ? . . 2 DCH 12 DC C6 12 DC H6 6 ? ? . . 2 DCH 12 DC C5 12 DC H5 7 ? ? . . 2 DCH 1 DG C8 1 DG H8 12 ? ? . . 2 DCH 2 DC C6 2 DC H6 14 ? ? . . 2 DCH 2 DC C5 2 DC H5 9 ? ? . . 2 DCH 3 DG C8 3 DG H8 7 ? ? . . 2 DCH 4 DT C6 4 DT H6 2 ? ? . . 2 DCH 4 DT C7 4 DT H71 0 ? ? . . 2 DCH 5 DT C6 5 DT H6 7 ? ? . . 2 DCH 5 DT C7 5 DT H71 2 ? ? . . 2 DCH 6 DT C6 6 DT H6 11 ? ? . . 2 DCH 7 DA C8 7 DA H8 13 ? ? . . 2 DCH 7 DA C2 7 DA H2 14 ? ? . . 2 DCH 8 DA C8 8 DA H8 5 ? ? . . 2 DCH 8 DA C2 8 DA H2 4 ? ? . . 2 DCH 9 DG C8 9 DG H8 0 ? ? . . 2 DCH 10 DC C6 10 DC H6 -7 ? ? . . 2 DCH 11 DG C8 11 DG H8 -6 ? ? . . 2 DCH 12 DG C8 12 DG H8 8 ? ? . . 2 DCH 1 DC C1' 1 DC H1' 8 ? ? . . 2 DCH 2 DC C1' 2 DC H1' 0 ? ? . . 2 DCH 3 DG C1' 3 DG H1' 11 ? ? . . 2 DCH 4 DC C1' 4 DC H1' 4 ? ? . . 2 DCH 5 DT C1' 5 DT H1' 12 ? ? . . 2 DCH 6 DT C1' 6 DT H1' 4 ? ? . . 2 DCH 7 DA C1' 7 DA H1' 0 ? ? . . 2 DCH 8 DA C1' 8 DA H1' 0 ? ? . . 2 DCH 9 DA C1' 9 DA H1' 8 ? ? . . 2 DCH 10 DC C1' 10 DC H1' 11 ? ? . . 2 DCH 11 DG C1' 11 DG H1' 6 ? ? . . 2 DCH 12 DC C1' 12 DC H1' 0 ? ? . . 2 DCH 1 DG C1' 1 DG H1' 4 ? ? . . 2 DCH 2 DC C1' 2 DC H1' -10 ? ? . . 2 DCH 3 DG C1' 3 DG H1' 1 ? ? . . 2 DCH 4 DT C1' 4 DT H1' 0 ? ? . . 2 DCH 5 DT C1' 5 DT H1' 0 ? ? . . 2 DCH 6 DT C1' 6 DT H1' 0 ? ? . . 2 DCH 7 DA C1' 7 DA H1' 11 ? ? . . 2 DCH 8 DA C1' 8 DA H1' 12 ? ? . . 2 DCH 9 DG C1' 9 DG H1' 1 ? ? . . 2 DCH 10 DC C1' 10 DC H1' 8 ? ? . . 2 DCH 11 DG C1' 11 DG H1' 7 ? ? . . 2 DCH 12 DG C1' 12 DG H1' 2 ? ? . . 2 DCH 3 DG C3' 3 DG H3' -3 ? ? . . 2 DCH 4 DC C3' 4 DC H3' 10 ? ? . . 2 DCH 5 DT C3' 5 DT H3' 5 ? ? . . 2 DCH 6 DT C3' 6 DT H3' -2 ? ? . . 2 DCH 7 DA C3' 7 DA H3' 3 ? ? . . 2 DCH 8 DA C3' 8 DA H3' 0 ? ? . . 2 DCH 9 DA C3' 9 DA H3' 3 ? ? . . 2 DCH 10 DC C3' 10 DC H3' 4 ? ? . . 2 DCH 5 DT C3' 5 DT H3' 2 ? ? . . 2 DCH 6 DT C3' 6 DT H3' -5 ? ? . . 2 DCH 7 DA C3' 7 DA H3' -5 ? ? . . 2 DCH 8 DA C3' 8 DA H3' 1 ? ? . . 2 DCH 9 DG C3' 9 DG H3' 11 ? ? . . 2 DCH 12 DG C3' 12 DG H3' 2 ? ? . . 2 DCH 3 DG C4' 3 DG H4' 4 ? ? . . 2 DCH 4 DC C4' 4 DC H4' 2 ? ? . . 2 DCH 5 DT C4' 5 DT H4' 0 ? ? . . 2 DCH 6 DT C4' 6 DT H4' 4 ? ? . . 2 DCH 7 DA C4' 7 DA H4' 6 ? ? . . 2 DCH 8 DA C4' 8 DA H4' 8 ? ? . . 2 DCH 9 DA C4' 9 DA H4' 2 ? ? . . 2 DCH 2 DC C4' 2 DC H4' 12 ? ? . . 2 DCH 3 DG C4' 3 DG H4' -5 ? ? . . 2 DCH 4 DT C4' 4 DT H4' 6 ? ? . . 2 DCH 5 DT C4' 5 DT H4' 3 ? ? . . 2 DCH 8 DA C4' 8 DA H4' 2 ? ? . . 2 DCH 9 DG C4' 9 DG H4' 5 ? ? . . 2 DCH 10 DC C4' 10 DC H4' 2 ? ? . . 2 DCH 11 DG C4' 11 DG H4' 0 ? ? . . 2 DCH 1 DC C6 1 DC H6 6 ? ? . . 2 DCH 3 DC C6 3 DC H6 -2 ? ? . . 2 DCH 6 DG C8 6 DG H8 7 ? ? . . 2 DCH 7 DT C6 7 DT H6 11 ? ? . . 2 DCH 8 DA C8 8 DA H8 9 ? ? . . 2 DCH 9 DC C6 9 DC H6 7 ? ? . . 2 DCH 10 DG C8 10 DG H8 -1 ? ? . . 2 DCH 12 DG C8 12 DG H8 7 ? ? . . 2 DCH 1 DC C6 1 DC H6 6 ? ? . . 2 DCH 4 DG C8 4 DG H8 11 ? ? . . 2 DCH 5 DT C6 5 DT H6 12 ? ? . . 2 DCH 6 DA C8 6 DA H8 9 ? ? . . 2 DCH 7 DC C6 7 DC H6 4 ? ? . . 2 DCH 8 DG C8 8 DG H8 7 ? ? . . 2 DCH 9 DT C6 9 DT H6 10 ? ? . . 2 DCH 11 DC C6 11 DC H6 7 ? ? . . 2 DCH 12 DG C8 12 DG H8 7 ? ? . . 2 DCH 1 DC C3' 1 DC H3' -1 ? ? . . 2 DCH 4 DA C3' 4 DA H3' -3 ? ? . . 2 DCH 5 DC C3' 5 DC H3' 3 ? ? . . 2 DCH 7 DT C3' 7 DT H3' 3 ? ? . . 2 DCH 9 DC C3' 9 DC H3' 5 ? ? . . 2 DCH 11 DC C3' 11 DC H3' 0 ? ? . . 2 DCH 1 DC C3' 1 DC H3' -1 ? ? . . 2 DCH 2 DG C3' 2 DG H3' -1 ? ? . . 2 DCH 5 DT C3' 5 DT H3' 3 ? ? . . 2 DCH 6 DA C3' 6 DA H3' 7 ? ? . . 2 DCH 7 DC C3' 7 DC H3' 5 ? ? . . 2 DCH 9 DT C3' 9 DT H3' 4 ? ? . . 2 DCH 11 DC C3' 11 DC H3' 0 ? ? . . 2 DCH 1 DC C4' 1 DC H4' 4 ? ? . . 2 DCH 2 DG C4' 2 DG H4' 6 ? ? . . 2 DCH 3 DC C4' 3 DC H4' -7 ? ? . . 2 DCH 7 DT C4' 7 DT H4' 3 ? ? . . 2 DCH 8 DA C4' 8 DA H4' 2 ? ? . . 2 DCH 12 DG C4' 12 DG H4' 5 ? ? . . 2 DCH 2 DG C4' 2 DG H4' 3 ? ? . . 2 DCH 3 DC C4' 3 DC H4' 0 ? ? . . 2 DCH 6 DA C4' 6 DA H4' 2 ? ? . . 2 DCH 9 DT C4' 9 DT H4' 3 ? ? . . 2 DCH 12 DG C4' 12 DG H4' 5 ? ? . . 2 stop_ save_