data_18891 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution structure of the Tetrahymena telomerase RNA stem IV terminal loop ; _BMRB_accession_number 18891 _BMRB_flat_file_name bmr18891.str _Entry_type original _Submission_date 2012-12-11 _Accession_date 2012-12-11 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Cash Darian D. . 2 Richards Rebecca J. . 3 Wu Haihong . . 4 Trantirek Lukas . . 5 O'Connor Catherine M . 6 Feigon Juli . . 7 Collins Kathleen . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 181 "13C chemical shifts" 141 "15N chemical shifts" 19 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2013-01-24 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 18892 'Solution structure of the helix II template boundary element from Tetrahymena telomerase RNA' stop_ _Original_release_date 2013-01-24 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Solution structure of the Tetrahymena telomerase RNA stem IV terminal loop' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 16809815 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Richards Rebecca J. . 2 Wu Haihong . . 3 Trantirek Lukas . . 4 O'Connor Catherine M . 5 Collins Kathleen . . 6 Feigon Juli . . stop_ _Journal_abbreviation RNA _Journal_volume 12 _Journal_issue 8 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 1475 _Page_last 1485 _Year 2006 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Tetrahymena telomerase RNA stem IV terminal loop' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label stemIV_telo_RNA $stemIV_telo_RNA stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_stemIV_telo_RNA _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common stemIV_telo_RNA _Molecular_mass 6650.027 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 21 _Mol_residue_sequence ; GGCGAUACACUAUUUAUCGC C ; loop_ _Residue_seq_code _Residue_label 1 G 2 G 3 C 4 G 5 A 6 U 7 A 8 C 9 A 10 C 11 U 12 A 13 U 14 U 15 U 16 A 17 U 18 C 19 G 20 C 21 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $stemIV_telo_RNA 'tetrahymena thermophila' 5911 Eukaryota . tetrahymena thermophila stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name _Details $stemIV_telo_RNA 'enzymatic synthesis' . n/a n/a . . 'in vitro transcription using T7 polymerase' stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $stemIV_telo_RNA . mM 0.6 1.0 unlabeled 'potassium chloride' 20 mM . . 'natural abundance' 'sodium azide' 0.2 % . . 'natural abundance' EDTA 50 uM . . 'natural abundance' 'sodium phosphate' 10 mM . . 'natural abundance' H2O 90 % . . 'natural abundance' D2O 10 % . . 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $stemIV_telo_RNA . mM 0.6 1.0 '13C,15N uniformly labelled' 'sodium phosphate' 10 mM . . 'natural abundance' 'potassium chloride' 20 mM . . 'natural abundance' 'sodium azide' 0.2 % . . 'natural abundance' EDTA 50 uM . . 'natural abundance' H2O 90 % . . 'natural abundance' D2O 10 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_X-PLOR_NIH _Saveframe_category software _Name 'X-PLOR NIH' _Version 2.9.8 loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task refinement 'structure solution' stop_ _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'data analysis' 'peak picking' stop_ _Details . save_ save_xwinnmr _Saveframe_category software _Name xwinnmr _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 800 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details . save_ save_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 500 _Details . save_ ############################# # NMR applied experiments # ############################# save_11echo_and_watergate_1 _Saveframe_category NMR_applied_experiment _Experiment_name '11echo and watergate' _Sample_label $sample_1 save_ save_2D_TOCSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D TOCSY' _Sample_label $sample_1 save_ save_2D_NOESY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label $sample_1 save_ save_2D_15N-HMQC,_2D_15N-CPMG-NOESY,_2D_JNN-HNN-COSY_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 15N-HMQC, 2D 15N-CPMG-NOESY, 2D JNN-HNN-COSY' _Sample_label $sample_2 save_ save_2D_13C-HSQCs_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 13C-HSQCs' _Sample_label $sample_2 save_ save_2D_HCCH_COSY,_3D_HCCH-TOCSY_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D HCCH COSY, 3D HCCH-TOCSY' _Sample_label $sample_2 save_ save_3D_1H,13C_HMQCs_7 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H,13C HMQCs' _Sample_label $sample_2 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 6.3 . pH pressure 1 . atm temperature 298 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 20 . mM pH 6.3 . M pressure 1 . atm temperature 283 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS C 13 'methyl protons' ppm 0.00 na indirect . . . 0.251449530 DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 DSS N 15 'methyl protons' ppm 0.00 na indirect . . . 0.101329118 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '11echo and watergate' '2D NOESY' '2D 13C-HSQCs' '2D HCCH COSY, 3D HCCH-TOCSY' stop_ loop_ _Sample_label $sample_1 $sample_2 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name stemIV_telo_RNA _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 G H1 H 12.088 . 1 2 1 1 G H1' H 5.759 . 1 3 1 1 G H2' H 4.880 . 1 4 1 1 G H3' H 4.661 . 1 5 1 1 G H4' H 4.501 . 1 6 1 1 G H5' H 4.342 . 2 7 1 1 G H5'' H 4.214 . 2 8 1 1 G H8 H 8.068 . 1 9 1 1 G C1' C 89.367 . 1 10 1 1 G C2' C 72.209 . 1 11 1 1 G C3' C 71.226 . 1 12 1 1 G C4' C 80.504 . 1 13 1 1 G C5' C 63.782 . 1 14 1 1 G C8 C 136.636 . 1 15 1 1 G N1 N 146.23 . 1 16 2 2 G H1 H 13.131 . 1 17 2 2 G H1' H 5.854 . 1 18 2 2 G H2' H 4.507 . 1 19 2 2 G H3' H 4.537 . 1 20 2 2 G H4' H 4.466 . 1 21 2 2 G H5' H 4.480 . 2 22 2 2 G H5'' H 4.163 . 2 23 2 2 G H8 H 7.560 . 1 24 2 2 G C1' C 90.237 . 1 25 2 2 G C2' C 72.618 . 1 26 2 2 G C3' C 69.837 . 1 27 2 2 G C4' C 79.579 . 1 28 2 2 G C5' C 62.937 . 1 29 2 2 G C8 C 136.970 . 1 30 2 2 G N1 N 148.23 . 1 31 3 3 C H1' H 5.500 . 1 32 3 3 C H2' H 4.558 . 1 33 3 3 C H3' H 4.436 . 1 34 3 3 C H4' H 4.353 . 1 35 3 3 C H5 H 5.189 . 1 36 3 3 C H5' H 4.504 . 2 37 3 3 C H5'' H 4.036 . 2 38 3 3 C H6 H 7.549 . 1 39 3 3 C H41 H 6.526 . 2 40 3 3 C H42 H 8.210 . 2 41 3 3 C C1' C 90.889 . 1 42 3 3 C C2' C 72.817 . 1 43 3 3 C C3' C 69.630 . 1 44 3 3 C C4' C 79.074 . 1 45 3 3 C C5' C 61.837 . 1 46 3 3 C N4 N 98.348 . 1 47 4 4 G H1 H 11.880 . 1 48 4 4 G H1' H 5.656 . 1 49 4 4 G H2' H 4.593 . 1 50 4 4 G H3' H 4.496 . 1 51 4 4 G H4' H 4.434 . 1 52 4 4 G H5' H 4.391 . 2 53 4 4 G H5'' H 4.063 . 2 54 4 4 G H8 H 7.453 . 1 55 4 4 G C1' C 90.229 . 1 56 4 4 G C2' C 72.527 . 1 57 4 4 G C3' C 70.446 . 1 58 4 4 G C4' C 79.350 . 1 59 4 4 G C5' C 63.575 . 1 60 4 4 G C8 C 133.536 . 1 61 4 4 G N1 N 146.09 . 1 62 5 5 A H1' H 5.874 . 1 63 5 5 A H2 H 7.447 . 1 64 5 5 A H2' H 4.413 . 1 65 5 5 A H3' H 4.537 . 1 66 5 5 A H4' H 4.440 . 1 67 5 5 A H5' H 4.527 . 2 68 5 5 A H5'' H 4.064 . 2 69 5 5 A H8 H 7.753 . 1 70 5 5 A C1' C 90.319 . 1 71 5 5 A C2 C 151.079 . 1 72 5 5 A C2' C 73.072 . 1 73 5 5 A C3' C 69.783 . 1 74 5 5 A C4' C 79.410 . 1 75 5 5 A C5' C 62.162 . 1 76 5 5 A C8 C 136.752 . 1 77 5 5 A N1 N 212.467 . 1 78 5 5 A N3 N 222.031 . 1 79 5 5 A N6 N 83.899 . 1 80 6 6 U H1' H 5.381 . 1 81 6 6 U H2' H 4.272 . 1 82 6 6 U H3 H 12.996 . 1 83 6 6 U H3' H 4.422 . 1 84 6 6 U H4' H 4.329 . 1 85 6 6 U H5 H 5.001 . 1 86 6 6 U H5' H 4.300 . 2 87 6 6 U H5'' H 3.978 . 2 88 6 6 U H6 H 7.502 . 1 89 6 6 U C1' C 90.229 . 1 90 6 6 U C2' C 72.663 . 1 91 6 6 U C3' C 69.421 . 1 92 6 6 U C4' C 79.244 . 1 93 6 6 U C5 C 100.262 . 1 94 6 6 U C5' C 62.815 . 1 95 6 6 U C6 C 138.213 . 1 96 6 6 U N3 N 161.25 . 1 97 7 7 A H1' H 5.903 . 1 98 7 7 A H2 H 7.013 . 1 99 7 7 A H2' H 4.362 . 1 100 7 7 A H3' H 4.527 . 1 101 7 7 A H4' H 4.435 . 1 102 7 7 A H5' H 4.414 . 2 103 7 7 A H5'' H 4.074 . 2 104 7 7 A H8 H 7.984 . 1 105 7 7 A H61 H 6.220 . 2 106 7 7 A H62 H 7.527 . 2 107 7 7 A C1' C 89.128 . 1 108 7 7 A C2 C 150.569 . 1 109 7 7 A C2' C 73.380 . 1 110 7 7 A C3' C 70.890 . 1 111 7 7 A C4' C 80.086 . 1 112 7 7 A C5' C 63.013 . 1 113 7 7 A C8 C 136.915 . 1 114 7 7 A N1 N 213.217 . 1 115 7 7 A N3 N 222.204 . 1 116 7 7 A N6 N 83.088 . 1 117 8 8 C H1' H 5.374 . 1 118 8 8 C H2' H 4.184 . 1 119 8 8 C H3' H 4.314 . 1 120 8 8 C H4' H 4.270 . 1 121 8 8 C H5 H 5.158 . 1 122 8 8 C H5' H 4.475 . 2 123 8 8 C H5'' H 3.990 . 2 124 8 8 C H6 H 7.259 . 1 125 8 8 C C1' C 90.412 . 1 126 8 8 C C2' C 72.984 . 1 127 8 8 C C3' C 70.808 . 1 128 8 8 C C4' C 80.012 . 1 129 8 8 C C5 C 94.703 . 1 130 8 8 C C5' C 61.783 . 1 131 8 8 C C6 C 138.448 . 1 132 9 9 A H1' H 5.885 . 1 133 9 9 A H2 H 7.762 . 1 134 9 9 A H2' H 4.396 . 1 135 9 9 A H3' H 4.500 . 1 136 9 9 A H4' H 4.359 . 1 137 9 9 A H5' H 4.286 . 2 138 9 9 A H5'' H 4.030 . 2 139 9 9 A H8 H 7.931 . 1 140 9 9 A C1' C 89.345 . 1 141 9 9 A C2 C 152.084 . 1 142 9 9 A C2' C 73.263 . 1 143 9 9 A C3' C 71.037 . 1 144 9 9 A C4' C 80.461 . 1 145 9 9 A C5' C 63.087 . 1 146 9 9 A C8 C 137.471 . 1 147 10 10 C H1' H 5.428 . 1 148 10 10 C H2' H 3.980 . 1 149 10 10 C H3' H 4.358 . 1 150 10 10 C H4' H 3.977 . 1 151 10 10 C H5 H 5.428 . 1 152 10 10 C H5' H 4.141 . 2 153 10 10 C H5'' H 3.916 . 2 154 10 10 C H6 H 7.336 . 1 155 10 10 C C1' C 88.587 . 1 156 10 10 C C2' C 73.278 . 1 157 10 10 C C3' C 73.171 . 1 158 10 10 C C4' C 81.413 . 1 159 10 10 C C5 C 94.408 . 1 160 10 10 C C5' C 63.857 . 1 161 10 10 C C6 C 139.629 . 1 162 11 11 U H1' H 5.698 . 1 163 11 11 U H2' H 4.131 . 1 164 11 11 U H3' H 4.393 . 1 165 11 11 U H4' H 4.131 . 1 166 11 11 U H5 H 5.702 . 1 167 11 11 U H5' H 3.824 . 2 168 11 11 U H5'' H 3.780 . 2 169 11 11 U H6 H 7.571 . 1 170 11 11 U C1' C 87.059 . 1 171 11 11 U C2' C 72.827 . 1 172 11 11 U C3' C 74.632 . 1 173 11 11 U C4' C 81.906 . 1 174 11 11 U C5 C 102.670 . 1 175 11 11 U C5' C 64.832 . 1 176 11 11 U C6 C 140.715 . 1 177 12 12 A H1' H 5.875 . 1 178 12 12 A H2 H 7.885 . 1 179 12 12 A H2' H 4.758 . 1 180 12 12 A H3' H 4.776 . 1 181 12 12 A H4' H 4.392 . 1 182 12 12 A H5' H 4.169 . 2 183 12 12 A H5'' H 4.031 . 2 184 12 12 A H8 H 8.214 . 1 185 12 12 A C1' C 88.015 . 1 186 12 12 A C2 C 152.197 . 1 187 12 12 A C2' C 72.968 . 1 188 12 12 A C3' C 73.064 . 1 189 12 12 A C4' C 81.805 . 1 190 12 12 A C5' C 64.799 . 1 191 12 12 A C8 C 139.412 . 1 192 13 13 U H1' H 5.862 . 1 193 13 13 U H2' H 4.325 . 1 194 13 13 U H3' H 4.619 . 1 195 13 13 U H4' H 4.439 . 1 196 13 13 U H5 H 5.667 . 1 197 13 13 U H5' H 4.251 . 2 198 13 13 U H5'' H 4.156 . 2 199 13 13 U H6 H 7.717 . 1 200 13 13 U C1' C 87.894 . 1 201 13 13 U C2' C 72.899 . 1 202 13 13 U C3' C 74.086 . 1 203 13 13 U C4' C 82.371 . 1 204 13 13 U C5 C 102.471 . 1 205 13 13 U C5' C 64.987 . 1 206 13 13 U C6 C 140.403 . 1 207 14 14 U H1' H 5.560 . 1 208 14 14 U H2' H 4.235 . 1 209 14 14 U H3' H 4.370 . 1 210 14 14 U H4' H 4.295 . 1 211 14 14 U H5 H 5.716 . 1 212 14 14 U H5' H 4.194 . 2 213 14 14 U H5'' H 4.081 . 2 214 14 14 U H6 H 7.715 . 1 215 14 14 U C1' C 90.499 . 1 216 14 14 U C2' C 72.673 . 1 217 14 14 U C3' C 71.986 . 1 218 14 14 U C4' C 80.889 . 1 219 14 14 U C5 C 101.658 . 1 220 14 14 U C5' C 64.589 . 1 221 14 14 U C6 C 140.660 . 1 222 15 15 U H1' H 5.651 . 1 223 15 15 U H2' H 4.607 . 1 224 15 15 U H3 H 13.295 . 1 225 15 15 U H3' H 4.615 . 1 226 15 15 U H4' H 4.397 . 1 227 15 15 U H5 H 5.702 . 1 228 15 15 U H5' H 4.370 . 2 229 15 15 U H5'' H 4.097 . 2 230 15 15 U H6 H 7.913 . 1 231 15 15 U C1' C 89.668 . 1 232 15 15 U C2' C 72.353 . 1 233 15 15 U C3' C 71.100 . 1 234 15 15 U C4' C 80.131 . 1 235 15 15 U C5 C 101.397 . 1 236 15 15 U C5' C 62.966 . 1 237 15 15 U C6 C 140.023 . 1 238 15 15 U N1 N 161.93 . 1 239 16 16 A H1' H 5.965 . 1 240 16 16 A H2 H 7.124 . 1 241 16 16 A H2' H 4.448 . 1 242 16 16 A H3' H 4.547 . 1 243 16 16 A H4' H 4.504 . 1 244 16 16 A H5' H 4.446 . 2 245 16 16 A H5'' H 4.228 . 2 246 16 16 A H8 H 8.244 . 1 247 16 16 A C1' C 90.234 . 1 248 16 16 A C2 C 150.469 . 1 249 16 16 A C2' C 72.722 . 1 250 16 16 A C3' C 70.539 . 1 251 16 16 A C4' C 79.788 . 1 252 16 16 A C5' C 63.681 . 1 253 16 16 A C8 C 137.707 . 1 254 16 16 A N1 N 212.794 . 1 255 16 16 A N3 N 222.146 . 1 256 16 16 A N6 N 83.920 . 1 257 17 17 U H1' H 5.399 . 1 258 17 17 U H2' H 4.278 . 1 259 17 17 U H3 H 13.890 . 1 260 17 17 U H3' H 4.353 . 1 261 17 17 U H4' H 4.336 . 1 262 17 17 U H5 H 4.960 . 1 263 17 17 U H5' H 4.476 . 2 264 17 17 U H5'' H 4.013 . 2 265 17 17 U H6 H 7.583 . 1 266 17 17 U C1' C 90.506 . 1 267 17 17 U C2' C 72.659 . 1 268 17 17 U C3' C 69.407 . 1 269 17 17 U C4' C 79.246 . 1 270 17 17 U C5 C 99.787 . 1 271 17 17 U C5' C 61.763 . 1 272 17 17 U C6 C 138.884 . 1 273 18 18 C H1' H 5.490 . 1 274 18 18 C H2' H 4.413 . 1 275 18 18 C H3' H 4.436 . 1 276 18 18 C H4' H 4.353 . 1 277 18 18 C H5 H 5.514 . 1 278 18 18 C H5' H 4.420 . 2 279 18 18 C H5'' H 4.019 . 2 280 18 18 C H6 H 7.699 . 1 281 18 18 C H41 H 6.591 . 2 282 18 18 C H42 H 8.121 . 2 283 18 18 C C1' C 90.889 . 1 284 18 18 C C2' C 72.702 . 1 285 18 18 C C3' C 69.630 . 1 286 18 18 C C4' C 79.031 . 1 287 18 18 C C5 C 95.215 . 1 288 18 18 C C5' C 62.341 . 1 289 18 18 C C6 C 138.432 . 1 290 18 18 C N4 N 97.728 . 1 291 19 19 G H1 H 12.584 . 1 292 19 19 G H1' H 5.597 . 1 293 19 19 G H2' H 4.442 . 1 294 19 19 G H3' H 4.468 . 1 295 19 19 G H4' H 4.376 . 1 296 19 19 G H5' H 4.421 . 2 297 19 19 G H5'' H 4.022 . 2 298 19 19 G H8 H 7.508 . 1 299 19 19 G C1' C 90.177 . 1 300 19 19 G C2' C 72.460 . 1 301 19 19 G C3' C 69.919 . 1 302 19 19 G C4' C 79.345 . 1 303 19 19 G C5' C 62.734 . 1 304 19 19 G C8 C 133.414 . 1 305 19 19 G N1 N 147.47 . 1 306 20 20 C H1' H 5.460 . 1 307 20 20 C H2' H 4.189 . 1 308 20 20 C H3' H 4.372 . 1 309 20 20 C H4' H 4.311 . 1 310 20 20 C H5 H 5.122 . 1 311 20 20 C H5' H 4.472 . 2 312 20 20 C H5'' H 3.965 . 2 313 20 20 C H6 H 7.623 . 1 314 20 20 C H41 H 6.686 . 2 315 20 20 C H42 H 8.334 . 2 316 20 20 C C1' C 91.331 . 1 317 20 20 C C2' C 72.875 . 1 318 20 20 C C3' C 69.055 . 1 319 20 20 C C4' C 79.166 . 1 320 20 20 C C5 C 94.071 . 1 321 20 20 C C5' C 61.546 . 1 322 20 20 C C6 C 138.406 . 1 323 20 20 C N4 N 99.108 . 1 324 21 21 C H1' H 5.691 . 1 325 21 21 C H2' H 3.941 . 1 326 21 21 C H3' H 4.096 . 1 327 21 21 C H4' H 4.085 . 1 328 21 21 C H5 H 5.445 . 1 329 21 21 C H5' H 4.401 . 2 330 21 21 C H5'' H 3.946 . 2 331 21 21 C H6 H 7.602 . 1 332 21 21 C H41 H 6.838 . 2 333 21 21 C H42 H 8.216 . 2 334 21 21 C C1' C 90.207 . 1 335 21 21 C C2' C 74.801 . 1 336 21 21 C C3' C 66.737 . 1 337 21 21 C C4' C 80.655 . 1 338 21 21 C C5 C 95.290 . 1 339 21 21 C C5' C 62.287 . 1 340 21 21 C C6 C 138.884 . 1 341 21 21 C N4 N 97.517 . 1 stop_ save_