data_18838 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution structure of the ID3 stem loop of domain 1 in the ai5gamma group II intron ; _BMRB_accession_number 18838 _BMRB_flat_file_name bmr18838.str _Entry_type original _Submission_date 2012-11-13 _Accession_date 2012-11-13 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details 'RNA stem loop' loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Popovic Milena . . 2 Greenbaum Nancy L. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 158 "13C chemical shifts" 132 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2014-02-17 update BMRB 'update entry citation' 2013-10-21 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title "Role of helical constraints of the EBS1-IBS1 duplex of a group II intron on demarcation of the 5' splice site." _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 24243113 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Popovic Milena . . 2 Greenbaum Nancy L. . stop_ _Journal_abbreviation RNA _Journal_name_full 'RNA (New York, N.Y.)' _Journal_volume 20 _Journal_issue 1 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 24 _Page_last 35 _Year 2014 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'ID3 stem loop of domain 1 in the ai5gamma group II intron' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'ID3 stem loop' $ID3 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_ID3 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class RNA _Name_common ID3 _Molecular_mass 132.116 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 23 _Mol_residue_sequence ; GGGUGUAUUGGAAAUGAGCA CCC ; loop_ _Residue_seq_code _Residue_author_seq_code _Residue_label 1 1 G 2 2 G 3 3 G 4 4 U 5 5 G 6 6 U 7 7 A 8 8 U 9 9 U 10 10 G 11 11 G 12 12 A 13 13 A 14 14 A 15 15 U 16 16 G 17 17 A 18 18 G 19 19 C 20 20 A 21 21 C 22 22 C 23 23 C stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $ID3 'Baker's yeast' 4932 Eukaryota Fungi Saccharomyces cerevisiae stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $ID3 'enzymatic semisynthesis' . Saccharomyces cerevisiae . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $ID3 . mM 0.03 0.9 'natural abundance' 'sodium chloride' 60 mM . . 'natural abundance' H2O 90 % . . 'natural abundance' D2O 10 % . . 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $ID3 . mM 0.03 0.9 'natural abundance' 'sodium chloride' 60 mM . . 'natural abundance' D2O 100 % . . 'natural abundance' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $ID3 . mM 0.3 0.5 '[U-100% 13C; U-100% 15N]' 'sodium chloride' 60 mM . . 'natural abundance' H2O 90 % . . 'natural abundance' D2O 10 % . . 'natural abundance' stop_ save_ save_sample_4 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $ID3 . mM 0.3 0.5 '[U-100% 13C; U-100% 15N]' 'potassium chloride' 60 mM . . 'natural abundance' D2O 100 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_CCPN _Saveframe_category software _Name CCPN _Version . loop_ _Vendor _Address _Electronic_address CCPN . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection processing stop_ _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_X-PLOR_NIH _Saveframe_category software _Name 'X-PLOR NIH' _Version . loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_2 save_ save_2D_1H-1H_TOCSY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_2 save_ save_2D_DQF-COSY_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D DQF-COSY' _Sample_label $sample_2 save_ save_2D_1H-15N_HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_3 save_ save_2D_1H-13C_HSQC_5 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC' _Sample_label $sample_4 save_ save_2D_1H-13C_HSQC_aliphatic_6 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC aliphatic' _Sample_label $sample_4 save_ save_2D_1H-13C_HSQC_aromatic_7 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-13C HSQC aromatic' _Sample_label $sample_4 save_ save_3D_1H-15N_NOESY_8 _Saveframe_category NMR_applied_experiment _Experiment_name '3D 1H-15N NOESY' _Sample_label $sample_3 save_ save_3D_HCCH-COSY_9 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCCH-COSY' _Sample_label $sample_4 save_ save_3D_HCCH-TOCSY_10 _Saveframe_category NMR_applied_experiment _Experiment_name '3D HCCH-TOCSY' _Sample_label $sample_4 save_ save_2D_1H-1H_NOESY_11 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.06 . M pH 6.4 . pH pressure 1 . atm temperature 277 . K stop_ save_ save_sample_conditions_2 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units 'ionic strength' 0.06 . M pH 6.4 . pH pressure 1 . atm temperature 303 . K stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H NOESY' stop_ loop_ _Sample_label $sample_2 $sample_1 stop_ _Sample_conditions_label $sample_conditions_2 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'ID3 stem loop' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 1 1 G H1' H 5.888 0.01 1 2 1 1 G H3' H 4.532 0.01 1 3 1 1 G H5' H 4.188 0.01 2 4 1 1 G H5'' H 4.326 0.01 2 5 1 1 G H8 H 8.121 0.01 1 6 1 1 G H2' H 4.950 0.01 1 7 2 2 G H1 H 12.893 0.01 1 8 2 2 G H3' H 4.560 0.01 1 9 2 2 G H5'' H 4.261 0.01 1 10 2 2 G H8 H 7.556 0.01 1 11 2 2 G H21 H 8.450 0.01 1 12 2 2 G H22 H 6.017 0.01 1 13 2 2 G H2' H 4.772 0.01 1 14 3 3 G H1 H 13.256 0.01 1 15 3 3 G H1' H 5.812 0.01 1 16 3 3 G H3' H 4.444 0.01 1 17 3 3 G H5' H 4.501 0.01 1 18 3 3 G H5'' H 4.101 0.01 1 19 3 3 G H8 H 7.141 0.01 1 20 3 3 G H21 H 8.518 0.01 1 21 3 3 G H22 H 6.098 0.01 1 22 3 3 G H2' H 4.518 0.01 1 23 4 4 U H1' H 5.591 0.01 1 24 4 4 U H3 H 13.732 0.01 1 25 4 4 U H3' H 4.525 0.01 1 26 4 4 U H4' H 4.450 0.01 1 27 4 4 U H5 H 5.135 0.01 1 28 4 4 U H6 H 7.695 0.01 1 29 4 4 U H2' H 4.761 0.01 1 30 5 5 G H1 H 12.750 0.01 1 31 5 5 G H1' H 5.812 0.01 1 32 5 5 G H3' H 4.443 0.01 1 33 5 5 G H5' H 4.496 0.01 1 34 5 5 G H5'' H 4.144 0.01 1 35 5 5 G H8 H 7.673 0.01 1 36 5 5 G H21 H 7.911 0.01 1 37 5 5 G H22 H 5.957 0.01 1 38 5 5 G H2' H 4.587 0.01 1 39 6 6 U H1' H 5.550 0.01 1 40 6 6 U H3 H 12.023 0.01 1 41 6 6 U H3' H 4.545 0.01 1 42 6 6 U H4' H 4.399 0.01 1 43 6 6 U H5 H 5.451 0.01 1 44 6 6 U H6 H 7.637 0.01 1 45 6 6 U H2' H 4.250 0.01 1 46 7 7 A H1' H 5.958 0.01 1 47 7 7 A H2 H 7.763 0.01 1 48 7 7 A H3' H 4.550 0.01 1 49 7 7 A H4' H 4.509 0.01 1 50 7 7 A H5' H 4.346 0.01 1 51 7 7 A H5'' H 4.146 0.01 1 52 7 7 A H8 H 8.247 0.01 1 53 7 7 A H2' H 4.621 0.01 1 54 8 8 U H1' H 5.621 0.01 1 55 8 8 U H3' H 4.492 0.01 1 56 8 8 U H4' H 4.368 0.01 1 57 8 8 U H5 H 5.473 0.01 1 58 8 8 U H5' H 4.094 0.01 1 59 8 8 U H6 H 7.626 0.01 1 60 8 8 U H2' H 4.286 0.01 1 61 9 9 U H1' H 5.711 0.01 1 62 9 9 U H3' H 4.400 0.01 1 63 9 9 U H5 H 5.668 0.01 1 64 9 9 U H5'' H 4.067 0.01 1 65 9 9 U H6 H 7.662 0.01 1 66 9 9 U H2' H 4.250 0.01 1 67 10 10 G H1' H 5.606 0.01 1 68 10 10 G H4' H 4.314 0.01 1 69 10 10 G H8 H 7.848 0.01 1 70 10 10 G H2' H 4.644 0.01 1 71 11 11 G H1' H 5.614 0.01 1 72 11 11 G H4' H 4.367 0.01 1 73 11 11 G H5'' H 4.099 0.01 1 74 11 11 G H8 H 7.859 0.01 1 75 11 11 G H2' H 4.729 0.01 1 76 12 12 A H1' H 5.826 0.01 1 77 12 12 A H2 H 7.955 0.01 1 78 12 12 A H4' H 4.378 0.01 1 79 12 12 A H5' H 4.109 0.01 2 80 12 12 A H5'' H 4.137 0.01 2 81 12 12 A H8 H 8.208 0.01 1 82 12 12 A H2' H 4.645 0.01 1 83 13 13 A H1' H 5.830 0.01 1 84 13 13 A H2 H 7.928 0.01 1 85 13 13 A H4' H 4.431 0.01 1 86 13 13 A H8 H 8.175 0.01 1 87 13 13 A H2' H 4.775 0.01 1 88 14 14 A H1' H 5.832 0.01 1 89 14 14 A H2 H 8.005 0.01 1 90 14 14 A H4' H 4.484 0.01 1 91 14 14 A H5' H 4.147 0.01 1 92 14 14 A H5'' H 4.184 0.01 1 93 14 14 A H8 H 8.175 0.01 1 94 14 14 A H2' H 4.589 0.01 1 95 15 15 U H1' H 5.739 0.01 1 96 15 15 U H4' H 4.367 0.01 1 97 15 15 U H5'' H 4.143 0.01 1 98 15 15 U H6 H 7.666 0.01 1 99 15 15 U H2' H 4.300 0.01 1 100 16 16 G H1' H 5.544 0.01 1 101 16 16 G H4' H 4.403 0.01 1 102 16 16 G H5' H 4.141 0.01 1 103 16 16 G H5'' H 4.225 0.01 1 104 16 16 G H8 H 7.810 0.01 1 105 16 16 G H2' H 4.643 0.01 1 106 17 17 A H1' H 6.006 0.01 1 107 17 17 A H2 H 8.013 0.01 1 108 17 17 A H5' H 4.248 0.01 1 109 17 17 A H5'' H 4.395 0.01 1 110 17 17 A H8 H 8.197 0.01 1 111 17 17 A H2' H 4.837 0.01 1 112 18 18 G H1 H 10.973 0.01 1 113 18 18 G H1' H 5.593 0.01 1 114 18 18 G H3' H 4.441 0.01 1 115 18 18 G H8 H 7.788 0.01 1 116 18 18 G H22 H 6.329 0.01 1 117 18 18 G H2' H 4.641 0.01 1 118 19 19 C H1' H 5.408 0.01 1 119 19 19 C H5 H 5.451 0.01 1 120 19 19 C H6 H 7.706 0.01 1 121 19 19 C H41 H 8.404 0.01 1 122 19 19 C H42 H 7.008 0.01 1 123 19 19 C H2' H 4.353 0.01 1 124 20 20 A H1' H 5.956 0.01 1 125 20 20 A H2 H 7.488 0.01 1 126 20 20 A H4' H 4.498 0.01 1 127 20 20 A H5' H 4.565 0.01 1 128 20 20 A H5'' H 4.149 0.01 1 129 20 20 A H8 H 8.092 0.01 1 130 20 20 A H61 H 8.146 0.01 1 131 20 20 A H62 H 6.364 0.01 1 132 20 20 A H2' H 4.560 0.01 1 133 21 21 C H1' H 5.431 0.01 1 134 21 21 C H3' H 4.375 0.01 1 135 21 21 C H4' H 4.397 0.01 1 136 21 21 C H5 H 5.261 0.01 1 137 21 21 C H5' H 4.509 0.01 1 138 21 21 C H5'' H 4.068 0.01 1 139 21 21 C H6 H 7.568 0.01 1 140 21 21 C H41 H 8.426 0.01 1 141 21 21 C H42 H 7.054 0.01 1 142 21 21 C H2' H 4.228 0.01 1 143 22 22 C H1' H 5.526 0.01 1 144 22 22 C H3' H 4.474 0.01 1 145 22 22 C H4' H 4.370 0.01 1 146 22 22 C H5 H 5.453 0.01 1 147 22 22 C H5' H 4.388 0.01 1 148 22 22 C H5'' H 4.042 0.01 1 149 22 22 C H6 H 7.778 0.01 1 150 22 22 C H41 H 8.546 0.01 1 151 22 22 C H42 H 6.942 0.01 1 152 22 22 C H2' H 4.253 0.01 1 153 23 23 C H1' H 5.783 0.01 1 154 23 23 C H3' H 4.185 0.01 1 155 23 23 C H4' H 4.197 0.01 1 156 23 23 C H5 H 5.516 0.01 1 157 23 23 C H6 H 7.689 0.01 1 158 23 23 C H2' H 4.014 0.01 1 159 2 2 G C1' C 92.75 0.1 1 160 2 2 G C2' C 74.79 0.1 1 161 2 2 G C3' C 75.22 0.1 1 162 2 2 G C4' C 81.80 0.1 1 163 2 2 G C5' C 65.37 0.1 1 164 2 2 G C8 C 136.57 0.1 1 165 3 3 G C1' C 93.21 0.1 1 166 3 3 G C2' C 75.22 0.1 1 167 3 3 G C3' C 72.31 0.1 1 168 3 3 G C4' C 84.50 0.1 1 169 3 3 G C5' C 65.70 0.1 1 170 3 3 G C8 C 136.23 0.1 1 171 4 4 U C1' C 93.46 0.1 1 172 4 4 U C2' C 74.71 0.1 1 173 4 4 U C3' C 73.04 0.1 1 174 4 4 U C4' C 84.26 0.1 1 175 4 4 U C5' C 65.39 0.1 1 176 4 4 U C5 C 103.01 0.1 1 177 4 4 U C6 C 140.41 0.1 1 178 5 5 G C1' C 93.01 0.1 1 179 5 5 G C2' C 73.00 0.1 1 180 5 5 G C4' C 81.33 0.1 1 181 5 5 G C5' C 67.23 0.1 1 182 5 5 G C8 C 136.08 0.1 1 183 6 6 U C1' C 92.18 0.1 1 184 6 6 U C2' C 75.50 0.1 1 185 6 6 U C3' C 74.20 0.1 1 186 6 6 U C4' C 83.00 0.1 1 187 6 6 U C5' C 64.93 0.1 1 188 6 6 U C5 C 104.35 0.1 1 189 6 6 U C6 C 140.81 0.1 1 190 7 7 A C1' C 92.75 0.1 1 191 7 7 A C2' C 77.63 0.1 1 192 7 7 A C3' C 71.51 0.1 1 193 7 7 A C4' C 84.27 0.1 1 194 7 7 A C5' C 66.91 0.1 1 195 7 7 A C2 C 154.67 0.1 1 196 7 7 A C8 C 141.40 0.1 1 197 8 8 U C1' C 90.40 0.1 1 198 8 8 U C2' C 74.20 0.1 1 199 8 8 U C3' C 73.50 0.1 1 200 8 8 U C4' C 81.80 0.1 1 201 8 8 U C5' C 67.23 0.1 1 202 8 8 U C5 C 104.21 0.1 1 203 9 9 U C1' C 89.95 0.1 1 204 9 9 U C2' C 75.22 0.1 1 205 9 9 U C3' C 75.22 0.1 1 206 9 9 U C4' C 83.80 0.1 1 207 9 9 U C5' C 67.74 0.1 1 208 9 9 U C5 C 104.89 0.1 1 209 9 9 U C6 C 142.67 0.1 1 210 10 10 G C1' C 91.08 0.1 1 211 10 10 G C2' C 73.33 0.1 1 212 10 10 G C3' C 71.99 0.1 1 213 10 10 G C5' C 63.91 0.1 1 214 10 10 G C8 C 139.02 0.1 1 215 11 11 G C1' C 90.40 0.1 1 216 11 11 G C2' C 75.72 0.1 1 217 11 11 G C3' C 71.80 0.1 1 218 11 11 G C4' C 85.00 0.1 1 219 11 11 G C5' C 66.45 0.1 1 220 11 11 G C8 C 139.69 0.1 1 221 12 12 A C1' C 90.35 0.1 1 222 12 12 A C4' C 83.95 0.1 1 223 12 12 A C5' C 64.69 0.1 1 224 12 12 A C2 C 154.69 0.1 1 225 12 12 A C8 C 141.84 0.1 1 226 13 13 A C1' C 90.77 0.1 1 227 13 13 A C4' C 81.79 0.1 1 228 13 13 A C5' C 65.71 0.1 1 229 13 13 A C2 C 154.69 0.1 1 230 13 13 A C8 C 141.41 0.1 1 231 14 14 A C1' C 90.73 0.1 1 232 14 14 A C2' C 74.71 0.1 1 233 14 14 A C3' C 73.00 0.1 1 234 14 14 A C4' C 85.00 0.1 1 235 14 14 A C5' C 64.00 0.1 1 236 14 14 A C2 C 154.69 0.1 1 237 14 14 A C8 C 141.41 0.1 1 238 15 15 U C1' C 90.07 0.1 1 239 15 15 U C2' C 75.72 0.1 1 240 15 15 U C3' C 75.22 0.1 1 241 15 15 U C4' C 84.30 0.1 1 242 15 15 U C5' C 66.72 0.1 1 243 15 15 U C5 C 105.01 0.1 1 244 15 15 U C6 C 142.67 0.1 1 245 16 16 G C1' C 90.82 0.1 1 246 16 16 G C3' C 75.22 0.1 1 247 16 16 G C4' C 83.99 0.1 1 248 17 17 A C3' C 74.71 0.1 1 249 17 17 A C4' C 83.45 0.1 1 250 17 17 A C5' C 65.89 0.1 1 251 17 17 A C2 C 154.85 0.1 1 252 17 17 A C8 C 140.78 0.1 1 253 18 18 G C1' C 92.57 0.1 1 254 18 18 G C3' C 74.00 0.1 1 255 18 18 G C4' C 82.35 0.1 1 256 19 19 C C1' C 93.60 0.1 1 257 19 19 C C2' C 75.54 0.1 1 258 19 19 C C3' C 71.75 0.1 1 259 19 19 C C4' C 81.82 0.1 1 260 19 19 C C5' C 63.62 0.1 1 261 19 19 C C5 C 97.59 0.1 1 262 19 19 C C6 C 141.21 0.1 1 263 20 20 A C1' C 92.76 0.1 1 264 20 20 A C2' C 75.33 0.1 1 265 20 20 A C3' C 72.16 0.1 1 266 20 20 A C4' C 82.04 0.1 1 267 20 20 A C5' C 64.88 0.1 1 268 20 20 A C2 C 153.57 0.1 1 269 20 20 A C8 C 139.41 0.1 1 270 21 21 C C1' C 93.58 0.1 1 271 21 21 C C2' C 75.42 0.1 1 272 21 21 C C3' C 71.91 0.1 1 273 21 21 C C4' C 81.76 0.1 1 274 21 21 C C5' C 64.49 0.1 1 275 21 21 C C5 C 97.02 0.1 1 276 21 21 C C6 C 140.72 0.1 1 277 22 22 C C1' C 94.23 0.1 1 278 22 22 C C2' C 75.52 0.1 1 279 22 22 C C3' C 71.96 0.1 1 280 22 22 C C4' C 81.83 0.1 1 281 22 22 C C5' C 65.05 0.1 1 282 22 22 C C5 C 97.59 0.1 1 283 22 22 C C6 C 141.41 0.1 1 284 23 23 C C1' C 92.81 0.1 1 285 23 23 C C2' C 77.45 0.1 1 286 23 23 C C3' C 69.62 0.1 1 287 23 23 C C4' C 83.42 0.1 1 288 23 23 C C5' C 64.82 0.1 1 289 23 23 C C5 C 97.99 0.1 1 290 23 23 C C6 C 141.82 0.1 1 stop_ save_