data_17537 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution structure of a Human minimembrane protein OST4 ; _BMRB_accession_number 17537 _BMRB_flat_file_name bmr17537.str _Entry_type original _Submission_date 2011-03-21 _Accession_date 2011-03-21 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Gayen Shovanlal . . 2 Kang Congbao . . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count assigned_chemical_shifts 1 stop_ loop_ _Data_type _Data_type_count "1H chemical shifts" 156 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2011-06-24 update BMRB 'update entry citation' 2011-06-03 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_citations _Saveframe_category entry_citation _Citation_full . _Citation_title 'Solution structure of a human minimembrane protein Ost4, a subunit of the oligosaccharyltransferase complex.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 21609714 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Gayen Shovanlal . . 2 Kang Congbao . . stop_ _Journal_abbreviation 'Biochem. Biophys. Res. Commun.' _Journal_name_full 'Biochemical and biophysical research communications' _Journal_volume 409 _Journal_issue 3 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 572 _Page_last 576 _Year 2011 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Human minimembrane protein OST4' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label 'Human minimembrane protein OST4' $OST4 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_OST4 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common 'Human minimembrane protein OST4' _Molecular_mass 4196.043 _Mol_thiol_state 'not present' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 37 _Mol_residue_sequence ; MITDVQLAIFANMLGVSLFL LVVLYHYVAVNNPKKQE ; loop_ _Residue_seq_code _Residue_label 1 MET 2 ILE 3 THR 4 ASP 5 VAL 6 GLN 7 LEU 8 ALA 9 ILE 10 PHE 11 ALA 12 ASN 13 MET 14 LEU 15 GLY 16 VAL 17 SER 18 LEU 19 PHE 20 LEU 21 LEU 22 VAL 23 VAL 24 LEU 25 TYR 26 HIS 27 TYR 28 VAL 29 ALA 30 VAL 31 ASN 32 ASN 33 PRO 34 LYS 35 LYS 36 GLN 37 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2LAT "Solution Structure Of A Human Minimembrane Protein Ost4" 100.00 37 100.00 100.00 2.31e-16 DBJ BAC24992 "unnamed protein product [Mus musculus]" 100.00 37 100.00 100.00 2.31e-16 DBJ BAC25041 "unnamed protein product [Mus musculus]" 100.00 37 100.00 100.00 2.31e-16 DBJ BAC25115 "unnamed protein product [Mus musculus]" 100.00 37 100.00 100.00 2.31e-16 DBJ BAC25288 "unnamed protein product [Mus musculus]" 100.00 37 100.00 100.00 2.31e-16 DBJ BAC25588 "unnamed protein product [Mus musculus]" 100.00 37 100.00 100.00 2.31e-16 GB AAH02177 "2310016E02Rik protein [Mus musculus]" 100.00 37 100.00 100.00 2.31e-16 GB AAH82316 "RIKEN cDNA 2310016E02 gene [Mus musculus]" 100.00 37 100.00 100.00 2.31e-16 GB AAI57815 "LOC100188932 protein [Rattus norvegicus]" 100.00 37 100.00 100.00 2.31e-16 GB ACH44975 "putative RIKEN cDNA 2310016E02 variant 1 [Taeniopygia guttata]" 100.00 37 100.00 100.00 2.31e-16 GB ACH44976 "putative RIKEN cDNA 2310016E02 variant 1 [Taeniopygia guttata]" 100.00 37 100.00 100.00 2.31e-16 REF NP_001128162 "dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 [Rattus norvegicus]" 100.00 37 100.00 100.00 2.31e-16 REF NP_001128163 "dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 [Rattus norvegicus]" 100.00 37 100.00 100.00 2.31e-16 REF NP_001128164 "dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 [Mus musculus]" 100.00 37 100.00 100.00 2.31e-16 REF NP_001128165 "dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 [Homo sapiens]" 100.00 37 100.00 100.00 2.31e-16 REF NP_001185794 "dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 [Taeniopygia guttata]" 100.00 37 100.00 100.00 2.31e-16 SP B0BLS0 "RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4" 100.00 37 100.00 100.00 2.31e-16 SP P0C6T2 "RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4" 100.00 37 100.00 100.00 2.31e-16 SP Q99LX8 "RecName: Full=Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4" 100.00 37 100.00 100.00 2.31e-16 TPG DAA24455 "TPA: hCG23722-like protein [Bos taurus]" 100.00 37 100.00 100.00 2.31e-16 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $OST4 Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $OST4 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $OST4 . mM 2 3 'natural abundance' CDC13 44 % . . 'natural abundance' Cd3OH 44 % . . 'natural abundance' H2O 12 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_TOPSPIN _Saveframe_category software _Name TOPSPIN _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task collection processing stop_ _Details . save_ save_SPARKY _Saveframe_category software _Name SPARKY _Version . loop_ _Vendor _Address _Electronic_address Goddard . . stop_ loop_ _Task 'chemical shift assignment' 'data analysis' stop_ _Details . save_ save_CYANA _Saveframe_category software _Name CYANA _Version 2.1 loop_ _Vendor _Address _Electronic_address 'Guntert, Mumenthaler and Wuthrich' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model Avance _Field_strength 700 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-1H_TOCSY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H TOCSY' _Sample_label $sample_1 save_ save_2D_1H-1H_NOESY_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-1H NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 298 . K pressure 1 . atm pH 7.0 . pH stop_ save_ #################### # NMR parameters # #################### ############################## # Assigned chemical shifts # ############################## ################################ # Chemical shift referencing # ################################ save_chemical_shift_reference_1 _Saveframe_category chemical_shift_reference _Details . loop_ _Mol_common_name _Atom_type _Atom_isotope_number _Atom_group _Chem_shift_units _Chem_shift_value _Reference_method _Reference_type _External_reference_sample_geometry _External_reference_location _External_reference_axis _Indirect_shift_ratio DSS H 1 'methyl protons' ppm 0.00 internal direct . . . 1.000000000 stop_ save_ ################################### # Assigned chemical shift lists # ################################### ################################################################### # Chemical Shift Ambiguity Index Value Definitions # # # # The values other than 1 are used for those atoms with different # # chemical shifts that cannot be assigned to stereospecific atoms # # or to specific residues or chains. # # # # Index Value Definition # # # # 1 Unique (including isolated methyl protons, # # geminal atoms, and geminal methyl # # groups with identical chemical shifts) # # (e.g. ILE HD11, HD12, HD13 protons) # # 2 Ambiguity of geminal atoms or geminal methyl # # proton groups (e.g. ASP HB2 and HB3 # # protons, LEU CD1 and CD2 carbons, or # # LEU HD11, HD12, HD13 and HD21, HD22, # # HD23 methyl protons) # # 3 Aromatic atoms on opposite sides of # # symmetrical rings (e.g. TYR HE1 and HE2 # # protons) # # 4 Intraresidue ambiguities (e.g. LYS HG and # # HD protons or TRP HZ2 and HZ3 protons) # # 5 Interresidue ambiguities (LYS 12 vs. LYS 27) # # 6 Intermolecular ambiguities (e.g. ASP 31 CA # # in monomer 1 and ASP 31 CA in monomer 2 # # of an asymmetrical homodimer, duplex # # DNA assignments, or other assignments # # that may apply to atoms in one or more # # molecule in the molecular assembly) # # 9 Ambiguous, specific ambiguity not defined # # # ################################################################### save_assigned_chem_shift_list_1 _Saveframe_category assigned_chemical_shifts _Details . loop_ _Experiment_label '2D 1H-1H TOCSY' '2D 1H-1H NOESY' stop_ loop_ _Sample_label $sample_1 stop_ _Sample_conditions_label $sample_conditions_1 _Chem_shift_reference_set_label $chemical_shift_reference_1 _Mol_system_component_name 'Human minimembrane protein OST4' _Text_data_format . _Text_data . loop_ _Atom_shift_assign_ID _Residue_author_seq_code _Residue_seq_code _Residue_label _Atom_name _Atom_type _Chem_shift_value _Chem_shift_value_error _Chem_shift_ambiguity_code 1 2 2 ILE H H 8.627 0.020 1 2 2 2 ILE HA H 4.467 0.020 1 3 3 3 THR H H 8.184 0.020 1 4 3 3 THR HA H 4.632 0.020 1 5 3 3 THR HG2 H 1.345 0.020 1 6 4 4 ASP H H 8.875 0.020 1 7 4 4 ASP HA H 4.538 0.020 1 8 4 4 ASP HB2 H 2.995 0.020 2 9 5 5 VAL H H 7.846 0.020 1 10 5 5 VAL HA H 3.772 0.020 1 11 5 5 VAL HB H 2.105 0.020 1 12 5 5 VAL HG1 H 1.164 0.020 2 13 6 6 GLN H H 7.762 0.020 1 14 6 6 GLN HA H 3.961 0.020 1 15 6 6 GLN HB2 H 2.099 0.020 2 16 6 6 GLN HG2 H 2.455 0.020 2 17 7 7 LEU H H 7.949 0.020 1 18 7 7 LEU HA H 4.199 0.020 1 19 7 7 LEU HB2 H 1.856 0.020 2 20 7 7 LEU HG H 1.066 0.020 1 21 8 8 ALA H H 8.043 0.020 1 22 8 8 ALA HA H 4.188 0.020 1 23 8 8 ALA HB H 1.658 0.020 1 24 9 9 ILE H H 8.282 0.020 1 25 9 9 ILE HA H 3.769 0.020 1 26 9 9 ILE HB H 2.047 0.020 1 27 9 9 ILE HG13 H 1.059 0.020 2 28 9 9 ILE HD1 H 0.942 0.020 1 29 10 10 PHE H H 8.374 0.020 1 30 10 10 PHE HA H 4.395 0.020 1 31 10 10 PHE HB3 H 3.383 0.020 2 32 11 11 ALA H H 8.927 0.020 1 33 11 11 ALA HA H 4.021 0.020 1 34 11 11 ALA HB H 1.663 0.020 1 35 12 12 ASN H H 8.136 0.020 1 36 12 12 ASN HA H 4.578 0.020 1 37 12 12 ASN HB2 H 3.061 0.020 2 38 12 12 ASN HB3 H 2.813 0.020 2 39 12 12 ASN HD21 H 7.476 0.020 2 40 12 12 ASN HD22 H 6.603 0.020 2 41 13 13 MET H H 8.564 0.020 1 42 13 13 MET HA H 4.178 0.020 1 43 13 13 MET HB2 H 2.227 0.020 2 44 13 13 MET HB3 H 2.357 0.020 2 45 13 13 MET HG2 H 2.826 0.020 2 46 14 14 LEU H H 8.624 0.020 1 47 14 14 LEU HA H 4.086 0.020 1 48 14 14 LEU HB2 H 1.704 0.020 2 49 14 14 LEU HB3 H 1.602 0.020 2 50 14 14 LEU HD1 H 1.219 0.020 2 51 14 14 LEU HD2 H 1.219 0.020 2 52 15 15 GLY H H 8.437 0.020 1 53 15 15 GLY HA2 H 3.966 0.020 2 54 15 15 GLY HA3 H 3.893 0.020 2 55 16 16 VAL H H 8.499 0.020 1 56 16 16 VAL HA H 3.848 0.020 1 57 16 16 VAL HB H 2.271 0.020 1 58 16 16 VAL HG1 H 1.214 0.020 2 59 16 16 VAL HG2 H 1.132 0.020 2 60 17 17 SER H H 8.087 0.020 1 61 17 17 SER HA H 4.283 0.020 1 62 17 17 SER HB2 H 4.027 0.020 2 63 18 18 LEU H H 8.318 0.020 1 64 18 18 LEU HA H 4.224 0.020 1 65 18 18 LEU HB2 H 1.963 0.020 2 66 18 18 LEU HB3 H 1.908 0.020 2 67 18 18 LEU HG H 1.790 0.020 1 68 18 18 LEU HD1 H 1.006 0.020 2 69 18 18 LEU HD2 H 1.006 0.020 2 70 19 19 PHE H H 8.201 0.020 1 71 19 19 PHE HA H 4.239 0.020 1 72 19 19 PHE HB2 H 3.480 0.020 2 73 19 19 PHE HB3 H 3.368 0.020 2 74 19 19 PHE HD1 H 7.278 0.020 3 75 19 19 PHE HD2 H 7.312 0.020 3 76 20 20 LEU H H 8.553 0.020 1 77 20 20 LEU HA H 3.941 0.020 1 78 20 20 LEU HB3 H 1.658 0.020 2 79 20 20 LEU HD1 H 1.045 0.020 2 80 20 20 LEU HD2 H 1.045 0.020 2 81 21 21 LEU H H 8.070 0.020 1 82 21 21 LEU HA H 4.149 0.020 1 83 21 21 LEU HB2 H 1.961 0.020 2 84 21 21 LEU HB3 H 1.886 0.020 2 85 21 21 LEU HD2 H 1.036 0.020 2 86 22 22 VAL H H 8.231 0.020 1 87 22 22 VAL HA H 3.730 0.020 1 88 22 22 VAL HB H 2.376 0.020 1 89 22 22 VAL HG1 H 1.203 0.020 2 90 22 22 VAL HG2 H 1.042 0.020 2 91 23 23 VAL H H 8.268 0.020 1 92 23 23 VAL HA H 3.558 0.020 1 93 23 23 VAL HB H 2.199 0.020 1 94 23 23 VAL HG1 H 0.984 0.020 2 95 23 23 VAL HG2 H 0.701 0.020 2 96 24 24 LEU H H 8.677 0.020 1 97 24 24 LEU HA H 4.205 0.020 1 98 24 24 LEU HB2 H 1.957 0.020 2 99 24 24 LEU HB3 H 1.918 0.020 2 100 24 24 LEU HG H 1.830 0.020 1 101 25 25 TYR H H 8.977 0.020 1 102 25 25 TYR HA H 4.185 0.020 1 103 25 25 TYR HB2 H 3.319 0.020 2 104 25 25 TYR HB3 H 3.224 0.020 2 105 25 25 TYR HD2 H 7.071 0.020 3 106 26 26 HIS H H 8.478 0.020 1 107 26 26 HIS HA H 4.176 0.020 1 108 26 26 HIS HB2 H 3.492 0.020 2 109 27 27 TYR H H 8.574 0.020 1 110 27 27 TYR HA H 4.088 0.020 1 111 27 27 TYR HB2 H 3.377 0.020 2 112 27 27 TYR HB3 H 3.228 0.020 2 113 27 27 TYR HD2 H 7.115 0.020 3 114 28 28 VAL H H 8.620 0.020 1 115 28 28 VAL HA H 3.598 0.020 1 116 28 28 VAL HB H 2.208 0.020 1 117 28 28 VAL HG1 H 1.029 0.020 2 118 28 28 VAL HG2 H 1.212 0.020 2 119 29 29 ALA H H 8.238 0.020 1 120 29 29 ALA HA H 4.032 0.020 1 121 29 29 ALA HB H 1.331 0.020 1 122 30 30 VAL H H 7.779 0.020 1 123 30 30 VAL HA H 3.911 0.020 1 124 30 30 VAL HB H 2.073 0.020 1 125 30 30 VAL HG1 H 0.904 0.020 2 126 30 30 VAL HG2 H 0.904 0.020 2 127 31 31 ASN H H 7.494 0.020 1 128 31 31 ASN HB2 H 2.665 0.020 2 129 31 31 ASN HB3 H 2.418 0.020 2 130 31 31 ASN HD21 H 6.231 0.020 2 131 31 31 ASN HD22 H 6.868 0.020 2 132 32 32 ASN H H 7.606 0.020 1 133 32 32 ASN HA H 4.858 0.020 1 134 32 32 ASN HB2 H 2.904 0.020 2 135 32 32 ASN HD21 H 7.769 0.020 2 136 32 32 ASN HD22 H 6.833 0.020 2 137 33 33 PRO HA H 4.484 0.020 1 138 33 33 PRO HD2 H 3.965 0.020 2 139 33 33 PRO HD3 H 3.860 0.020 2 140 34 34 LYS H H 8.091 0.020 1 141 34 34 LYS HA H 4.362 0.020 1 142 34 34 LYS HB2 H 1.961 0.020 2 143 34 34 LYS HG2 H 1.535 0.020 2 144 34 34 LYS HG3 H 1.476 0.020 2 145 34 34 LYS HD2 H 1.797 0.020 2 146 35 35 LYS H H 7.911 0.020 1 147 35 35 LYS HA H 4.302 0.020 1 148 35 35 LYS HB2 H 1.859 0.020 2 149 35 35 LYS HB3 H 1.941 0.020 2 150 35 35 LYS HG3 H 1.510 0.020 2 151 35 35 LYS HD2 H 1.792 0.020 2 152 36 36 GLN H H 8.142 0.020 1 153 36 36 GLN HA H 4.440 0.020 1 154 36 36 GLN HB2 H 2.200 0.020 2 155 36 36 GLN HB3 H 2.034 0.020 2 156 36 36 GLN HG2 H 2.410 0.020 2 stop_ save_