data_17339 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; The Solution Structure of a Fungal AREA Protein-DNA Complex: An Alternative Binding Mode for a Basic Carboxyl Tail of GATA factors ; _BMRB_accession_number 17339 _BMRB_flat_file_name bmr17339.str _Entry_type original _Submission_date 2010-12-02 _Accession_date 2010-12-02 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Starich Mary R. . 2 Wikstrom Mats . . 3 Arst Herbert N. Jr. 4 Clore G. M. . 5 Gronenborn Angela M. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count chemical_rates 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2012-06-06 original author . stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'The solution structure of a fungal AREA protein-DNA complex: an alternative binding mode for the basic carboxyl tail of GATA factors.' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 9533883 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Starich Mary R. . 2 Wikstrom Mats . . 3 Arst Herbert N. Jr. 4 Clore G. M. . 5 Gronenborn Angela M. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_name_full 'Journal of molecular biology' _Journal_volume 277 _Journal_issue 3 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 605 _Page_last 620 _Year 1998 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'AREA/GATA complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label AREA $AREA GATA $GATA stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_AREA _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common AREA _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 66 _Mol_residue_sequence ; MKNGEQNGPTTCTNCFTQTT PLWRRNPEGQPLCNACGLFL KLHGVVRPLSLKTDVIKKRN RNSANS ; loop_ _Residue_seq_code _Residue_label 1 MET 2 LYS 3 ASN 4 GLY 5 GLU 6 GLN 7 ASN 8 GLY 9 PRO 10 THR 11 THR 12 CYS 13 THR 14 ASN 15 CYS 16 PHE 17 THR 18 GLN 19 THR 20 THR 21 PRO 22 LEU 23 TRP 24 ARG 25 ARG 26 ASN 27 PRO 28 GLU 29 GLY 30 GLN 31 PRO 32 LEU 33 CYS 34 ASN 35 ALA 36 CYS 37 GLY 38 LEU 39 PHE 40 LEU 41 LYS 42 LEU 43 HIS 44 GLY 45 VAL 46 VAL 47 ARG 48 PRO 49 LEU 50 SER 51 LEU 52 LYS 53 THR 54 ASP 55 VAL 56 ILE 57 LYS 58 LYS 59 ARG 60 ASN 61 ARG 62 ASN 63 SER 64 ALA 65 ASN 66 SER stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-07-08 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 2VUS "Crystal Structure Of Unliganded Nmra-Area Zinc Finger Complex" 65.15 43 100.00 100.00 2.67e-22 PDB 2VUT "Crystal Structure Of Nad-Bound Nmra-Area Zinc Finger Complex" 65.15 43 100.00 100.00 2.67e-22 PDB 2VUU "Crystal Structure Of Nadp-Bound Nmra-Area Zinc Finger Complex" 65.15 43 100.00 100.00 2.67e-22 PDB 4GAT "Solution Nmr Structure Of The Wild Type Dna Binding Domain Of Area Complexed To A 13bp Dna Containing A Cgata Site, Regularized" 100.00 66 100.00 100.00 9.23e-40 PDB 5GAT "Solution Nmr Structure Of The Wild Type Dna Binding Domain Of Area Complexed To A 13bp Dna Containing A Cgata Site, 35 Structur" 100.00 66 100.00 100.00 9.23e-40 PDB 6GAT "Solution Nmr Structure Of The L22v Mutant Dna Binding Domain Of Area Complexed To A 13 Bp Dna Containing A Tgata Site, Regulari" 100.00 66 98.48 100.00 2.30e-39 PDB 7GAT "Solution Nmr Structure Of The L22v Mutant Dna Binding Domain Of Area Complexed To A 13 Bp Dna Containing A Tgata Site, 34 Struc" 100.00 66 98.48 100.00 2.30e-39 DBJ GAN02876 "hypothetical protein MAM1_0029d02325 [Mucor ambiguus]" 57.58 722 97.37 97.37 1.42e-15 EMBL CAA36731 "areA [Aspergillus nidulans]" 98.48 876 100.00 100.00 2.83e-34 GB AAU05980 "global nitrogen regulator protein, partial [Colletotrichum gloeosporioides]" 71.21 47 100.00 100.00 1.62e-25 GB AEO65119 "hypothetical protein THITE_37279 [Thielavia terrestris NRRL 8126]" 80.30 988 100.00 100.00 1.06e-26 GB EAA60701 "AREA_EMENI Nitrogen regulatory protein areA [Aspergillus nidulans FGSC A4]" 98.48 876 100.00 100.00 2.83e-34 GB EAW08915 "GATA transcriptional activator AreA [Aspergillus clavatus NRRL 1]" 100.00 879 96.97 98.48 4.07e-32 GB EGS22804 "putative sequence-specific DNA binding protein [Chaetomium thermophilum var. thermophilum DSM 1495]" 80.30 928 100.00 100.00 6.27e-29 REF XP_001270341 "GATA transcriptional activator AreA [Aspergillus clavatus NRRL 1]" 100.00 879 96.97 98.48 4.07e-32 REF XP_003651455 "hypothetical protein THITE_37279 [Thielavia terrestris NRRL 8126]" 80.30 988 100.00 100.00 1.06e-26 REF XP_006691796 "putative sequence-specific DNA binding protein [Chaetomium thermophilum var. thermophilum DSM 1495]" 80.30 928 100.00 100.00 6.27e-29 REF XP_681936 "AREA_EMENI Nitrogen regulatory protein areA [Aspergillus nidulans FGSC A4]" 98.48 876 100.00 100.00 2.83e-34 SP P17429 "RecName: Full=Nitrogen regulatory protein areA" 98.48 876 100.00 100.00 2.83e-34 TPE CBF78221 "TPA: Nitrogen regulatory protein areA [Source:UniProtKB/Swiss-Prot;Acc:P17429] [Aspergillus nidulans FGSC A4]" 98.48 876 100.00 100.00 2.83e-34 stop_ save_ save_GATA _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class DNA _Name_common GATA _Molecular_mass . _Mol_thiol_state 'not reported' _Details . _Residue_count 26 _Mol_residue_sequence ; CAGCGATAGAGACGTCGCTA TCTCTG ; loop_ _Residue_seq_code _Residue_label 1 DC 2 DA 3 DG 4 DC 5 DG 6 DA 7 DT 8 DA 9 DG 10 DA 11 DG 12 DA 13 DC 14 DG 15 DT 16 DC 17 DG 18 DC 19 DT 20 DA 21 DT 22 DC 23 DT 24 DC 25 DT 26 DG stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $AREA 'Aspergillus nidulans' 162425 Eukaryota Fungi . . $GATA . . . . . . stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Variant _Vector_name $AREA 'recombinant technology' . . . BL21 DE3 pET11a $GATA 'obtained from a vendor' . . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'AREA has zinc bound and was in a complex with GATA' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $AREA 2 mM [U-15N] $GATA 2 mM 'natural abundance' 'sodium chloride' 12 mM 'natural abundance' 'zinc chloride' 2.2 mM 'natural abundance' 'sodium azide' 5.0 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details 'AREA has zinc bound and was in a complex with GATA' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $AREA 2 mM '[U-13C; U-15N]' $GATA 2 mM 'natural abundance' 'sodium chloride' 12 mM 'natural abundance' 'zinc chloride' 2.2 mM 'natural abundance' 'sodium azide' 5.0 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ save_sample_3 _Saveframe_category sample _Sample_type solution _Details 'AREA has zinc bound and was in a complex with GATA' loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $AREA 2 mM '[U-13C; U-15N]' $GATA 2 mM 'natural abundance' 'sodium chloride' 12 mM 'natural abundance' 'zinc chloride' 2.2 mM 'natural abundance' 'sodium azide' 5.0 mM 'natural abundance' D2O 99.996 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task processing stop_ _Details . save_ save_PIPP _Saveframe_category software _Name PIPP _Version . loop_ _Vendor _Address _Electronic_address Garrett . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_CAPP _Saveframe_category software _Name CAPP _Version . loop_ _Vendor _Address _Electronic_address Garrett . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_STAPP _Saveframe_category software _Name STAPP _Version . loop_ _Vendor _Address _Electronic_address '(Garrett et al., 1991)' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AMX _Field_strength 500 _Details . save_ save_spectrometer_2 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 500 _Details . save_ save_spectrometer_3 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AMX _Field_strength 600 _Details . save_ save_spectrometer_4 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 600 _Details . save_ save_spectrometer_5 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DMX _Field_strength 750 _Details . save_ save_spectrometer_6 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model AMX _Field_strength 360 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_2 save_ save_2D_1H-15N_HSQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_3 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 298 . K pH 6.5 . pH pressure 1 . atm stop_ save_