data_17334 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; EGF-like Domain Calcium Affinity Modulated by N-terminal Domain Linkage in Human Fibrillin-1 ; _BMRB_accession_number 17334 _BMRB_flat_file_name bmr17334.str _Entry_type original _Submission_date 2010-12-02 _Accession_date 2010-12-02 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Smallridge Rachel S. . 2 Whiteman Pat . . 3 Doering Klaus . . 4 Handford Penny A. . 5 Downing A. K. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count binding_constants 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2012-06-06 original author . stop_ _Original_release_date 2012-06-06 save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'EGF-like Domain Calcium Affinity Modulated by N-terminal Domain Linkage in Human Fibrillin-1' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 10024441 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Smallridge Rachel S. . 2 Whiteman Pat . . 3 Doering Klaus . . 4 Handford Penny A. . 5 Downing A. K. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_volume 286 _Journal_issue . _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 661 _Page_last 668 _Year 1999 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'EGF/CA compplex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label cbEGF12 $EGF cbEGF13 $EGF CA $CA stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_EGF _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common EGF _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 86 _Mol_residue_sequence ; TDIDECRISPDLCGRGQCVN TPGDFECKCDEGYESGFMMM KNCMDIDECQRDPLLCRGGV CHNTEGSYRCECPPGHQLSP NISACI ; loop_ _Residue_seq_code _Residue_label 1 THR 2 ASP 3 ILE 4 ASP 5 GLU 6 CYS 7 ARG 8 ILE 9 SER 10 PRO 11 ASP 12 LEU 13 CYS 14 GLY 15 ARG 16 GLY 17 GLN 18 CYS 19 VAL 20 ASN 21 THR 22 PRO 23 GLY 24 ASP 25 PHE 26 GLU 27 CYS 28 LYS 29 CYS 30 ASP 31 GLU 32 GLY 33 TYR 34 GLU 35 SER 36 GLY 37 PHE 38 MET 39 MET 40 MET 41 LYS 42 ASN 43 CYS 44 MET 45 ASP 46 ILE 47 ASP 48 GLU 49 CYS 50 GLN 51 ARG 52 ASP 53 PRO 54 LEU 55 LEU 56 CYS 57 ARG 58 GLY 59 GLY 60 VAL 61 CYS 62 HIS 63 ASN 64 THR 65 GLU 66 GLY 67 SER 68 TYR 69 ARG 70 CYS 71 GLU 72 CYS 73 PRO 74 PRO 75 GLY 76 HIS 77 GLN 78 LEU 79 SER 80 PRO 81 ASN 82 ILE 83 SER 84 ALA 85 CYS 86 ILE stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2013-12-04 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1LMJ "Nmr Study Of The Fibrillin-1 Cbegf12-13 Pair Of Ca2+ Binding Epidermal Growth Factor-like Domains" 98.84 86 100.00 100.00 5.32e-54 stop_ save_ ############# # Ligands # ############# save_CA _Saveframe_category ligand _Mol_type non-polymer _Name_common "CA (CALCIUM ION)" _BMRB_code . _PDB_code CA _Molecular_mass 40.078 _Mol_charge 2 _Mol_paramagnetic no _Mol_aromatic no _Details ; Information obtained from PDB's Chemical Component Dictionary at http://wwpdb-remediation.rutgers.edu/downloads.html Downloaded on Tue Jun 9 13:21:54 2009 ; loop_ _Atom_name _PDB_atom_name _Atom_type _Atom_chirality _Atom_charge _Atom_oxidation_number _Atom_unpaired_electrons CA CA CA . 2 . ? stop_ _Mol_thiol_state 'not present' _Sequence_homology_query_date . save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $EGF Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $EGF 'recombinant technology' . Escherichia coli . pQE-30 stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details 'Ca titration' loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $EGF 131 uM . . 'natural abundance' 'sodium chloride' 150 mM . . 'natural abundance' Tris 4.55 mM . . 'natural abundance' $CA . mM 0 277.72 'natural abundance' H2O 90 % . . 'natural abundance' D2O 10 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_software _Saveframe_category software _Name software _Version . _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer GE _Model Omega _Field_strength 600 _Details home-built save_ ############################# # NMR applied experiments # ############################# save_2D_NOESY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D NOESY' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units pH 6.5 . pH pressure 1 . atm temperature 306 . K stop_ save_