data_16752 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Mapping the Interacting Regions between Troponins T and C ; _BMRB_accession_number 16752 _BMRB_flat_file_name bmr16752.str _Entry_type new _Submission_date 2010-03-03 _Accession_date 2010-03-03 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Blumenschein Tharin M.A. . 2 Tripet Brian P. . 3 Hodges Robert S. . 4 Sykes Brian D. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count binding_constants 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-06-03 update BMRB 'edit assembly name' 2010-03-24 original author 'original release' stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Mapping the Interacting Regions between Troponins T and C' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 11473120 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Blumenschein Tharin M.A. . 2 Tripet Brian P. . 3 Hodges Robert S. . 4 Sykes Brian D. . stop_ _Journal_abbreviation 'J. Biol. Chem.' _Journal_volume 276 _Journal_issue 39 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 36606 _Page_last 36612 _Year 2001 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Troponins T and C' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label TnC $TnC TnT(160-193) $TnT(160-193) stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_TnC _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common TnC _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 161 _Mol_residue_sequence ; MDDIYKAAVEQLTEEQKNEF KAAFDIFVLGAEDGCISTKE LGKVMRMLGQNPTPEELQEM IDEVDEDGSGTVDFDEFLVM MVRCMKDDSKGKTEEELSDL FRMFDKNADGYIDLEELKIM LQATGETITEDDIEELMKDG DKNNDGRIDYDEFLEFMKGV E ; loop_ _Residue_seq_code _Residue_label 1 MET 2 ASP 3 ASP 4 ILE 5 TYR 6 LYS 7 ALA 8 ALA 9 VAL 10 GLU 11 GLN 12 LEU 13 THR 14 GLU 15 GLU 16 GLN 17 LYS 18 ASN 19 GLU 20 PHE 21 LYS 22 ALA 23 ALA 24 PHE 25 ASP 26 ILE 27 PHE 28 VAL 29 LEU 30 GLY 31 ALA 32 GLU 33 ASP 34 GLY 35 CYS 36 ILE 37 SER 38 THR 39 LYS 40 GLU 41 LEU 42 GLY 43 LYS 44 VAL 45 MET 46 ARG 47 MET 48 LEU 49 GLY 50 GLN 51 ASN 52 PRO 53 THR 54 PRO 55 GLU 56 GLU 57 LEU 58 GLN 59 GLU 60 MET 61 ILE 62 ASP 63 GLU 64 VAL 65 ASP 66 GLU 67 ASP 68 GLY 69 SER 70 GLY 71 THR 72 VAL 73 ASP 74 PHE 75 ASP 76 GLU 77 PHE 78 LEU 79 VAL 80 MET 81 MET 82 VAL 83 ARG 84 CYS 85 MET 86 LYS 87 ASP 88 ASP 89 SER 90 LYS 91 GLY 92 LYS 93 THR 94 GLU 95 GLU 96 GLU 97 LEU 98 SER 99 ASP 100 LEU 101 PHE 102 ARG 103 MET 104 PHE 105 ASP 106 LYS 107 ASN 108 ALA 109 ASP 110 GLY 111 TYR 112 ILE 113 ASP 114 LEU 115 GLU 116 GLU 117 LEU 118 LYS 119 ILE 120 MET 121 LEU 122 GLN 123 ALA 124 THR 125 GLY 126 GLU 127 THR 128 ILE 129 THR 130 GLU 131 ASP 132 ASP 133 ILE 134 GLU 135 GLU 136 LEU 137 MET 138 LYS 139 ASP 140 GLY 141 ASP 142 LYS 143 ASN 144 ASN 145 ASP 146 GLY 147 ARG 148 ILE 149 ASP 150 TYR 151 ASP 152 GLU 153 PHE 154 LEU 155 GLU 156 PHE 157 MET 158 LYS 159 GLY 160 VAL 161 GLU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-10-07 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 16190 cNTnC 55.28 89 97.75 97.75 1.85e-52 BMRB 17103 cNTnC 55.28 89 100.00 100.00 9.84e-55 BMRB 19789 cNTnC 55.28 89 100.00 100.00 9.84e-55 BMRB 25034 cChimera 55.28 141 98.88 98.88 5.84e-54 BMRB 25035 cChimeraX 55.28 141 98.88 98.88 4.45e-54 BMRB 25120 cTnC 100.00 161 97.52 98.76 2.30e-104 BMRB 25495 cNTnC 55.28 89 100.00 100.00 9.84e-55 PDB 1AJ4 "Structure Of Calcium-Saturated Cardiac Troponin C, Nmr, 1 Structure" 98.76 161 98.74 98.74 2.70e-104 PDB 1AP4 "Regulatory Domain Of Human Cardiac Troponin C In The Calcium-Saturated State, Nmr, 40 Structures" 55.28 89 100.00 100.00 9.84e-55 PDB 1DTL "Crystal Structure Of Calcium-Saturated (3ca2+) Cardiac Troponin C Complexed With The Calcium Sensitizer Bepridil At 2.15 A Reso" 100.00 161 98.14 98.76 4.27e-105 PDB 1FI5 "Nmr Structure Of The C Terminal Domain Of Cardiac Troponin C Bound To The N Terminal Domain Of Cardiac Troponin I." 50.31 81 100.00 100.00 9.53e-47 PDB 1J1D "Crystal Structure Of The 46kda Domain Of Human Cardiac Troponin In The Ca2+ Saturated Form" 100.00 161 98.14 98.76 4.27e-105 PDB 1J1E "Crystal Structure Of The 52kda Domain Of Human Cardiac Troponin In The Ca2+ Saturated Form" 100.00 161 98.14 98.76 4.27e-105 PDB 1LA0 "Solution Structure Of Calcium Saturated Cardiac Troponin C In The Troponin C-Troponin I Complex" 100.00 161 100.00 100.00 1.41e-107 PDB 1LXF "Structure Of The Regulatory N-Domain Of Human Cardiac Troponin C In Complex With Human Cardiac Troponin-I(147- 163) And Bepridi" 55.28 89 100.00 100.00 9.84e-55 PDB 1MXL "Structure Of Cardiac Troponin C-troponin I Complex" 55.28 89 100.00 100.00 9.84e-55 PDB 1SBJ "Nmr Structure Of The Mg2+-Loaded C Terminal Domain Of Cardiac Troponin C Bound To The N Terminal Domain Of Cardiac Troponin I" 50.31 81 100.00 100.00 9.53e-47 PDB 1SCV "Nmr Structure Of The C Terminal Domain Of Cardiac Troponin C Bound To The N Terminal Domain Of Cardiac Troponin I" 50.31 81 100.00 100.00 9.53e-47 PDB 1SPY "Regulatory Domain Of Human Cardiac Troponin C In The Calcium-Free State, Nmr, 40 Structures" 55.28 89 100.00 100.00 9.84e-55 PDB 1WRK "Crystal Structure Of The N-Terminal Domain Of Human Cardiac Troponin C In Complex With Trifluoperazine (Orthrombic Crystal Form" 54.66 88 97.73 97.73 1.11e-51 PDB 1WRL "Crystal Structure Of The N-Terminal Domain Of Human Cardiac Troponin C In Complex With Trifluoperazine (Monoclinic Crystal Form" 54.66 88 97.73 97.73 1.11e-51 PDB 2CTN "Structure Of Calcium-Saturated Cardiac Troponin C, Nmr, 30 Structures" 54.04 89 97.70 97.70 3.55e-51 PDB 2JXL "Solution Structure Of Cardiac N-Domain Troponin C Mutant F77w-V82a" 55.28 89 97.75 98.88 7.84e-54 PDB 2KFX "Structure Of The N-Terminal Domain Of Human Cardiac Troponin C Bound To Calcium Ion And To The Inhibitor W7" 55.28 89 97.75 97.75 1.85e-52 PDB 2KGB "Nmr Solution Of The Regulatory Domain Cardiac F77w-Troponin C In Complex With The Cardiac Troponin I 144-163 Switch Peptide" 55.28 89 98.88 100.00 2.18e-54 PDB 2KRD "Solution Structure Of The Regulatory Domain Of Human Cardiac C In Complex With The Switch Region Of Cardiac Troponin I A" 55.28 89 100.00 100.00 9.84e-55 PDB 2L1R "The Structure Of The Calcium-Sensitizer, Dfbp-O, In Complex With The N-Domain Of Troponin C And The Switch Region Of Troponin I" 55.28 89 100.00 100.00 9.84e-55 PDB 2MKP "N Domain Of Cardiac Troponin C Bound To The Switch Fragment Of Fast Skeletal Troponin I At Ph 6" 55.28 89 100.00 100.00 9.84e-55 PDB 2MZP "Structure And Dynamics Of The Acidosis-resistant A162h Mutant Of The Switch Region Of Troponin I Bound To The Regulatory Domain" 55.28 89 100.00 100.00 9.84e-55 PDB 3SD6 "Crystal Structure Of The Amino-terminal Domain Of Human Cardiac Troponin C In Complex With Cadmium At 1.4 Resolution." 55.28 89 100.00 100.00 9.84e-55 PDB 3SWB "Crystal Structure Of The Amino-terminal Domain Of Human Cardiac Troponin C In Complex With Cadmium At 1.7 A Resolution" 55.28 89 100.00 100.00 9.84e-55 PDB 4GJE "Crystal Structure Of The Refolded Amino-terminal Domain Of Human Cardiac Troponin C In Complex With Cadmium" 55.28 89 100.00 100.00 9.84e-55 DBJ BAA02369 "cardiac troponin C [Gallus gallus]" 100.00 161 100.00 100.00 1.41e-107 DBJ BAG36483 "unnamed protein product [Homo sapiens]" 100.00 161 98.76 100.00 1.29e-106 EMBL CAA30736 "unnamed protein product [Homo sapiens]" 100.00 161 98.76 100.00 1.29e-106 EMBL CAG46663 "TNNC1 [Homo sapiens]" 100.00 161 98.76 100.00 1.29e-106 EMBL CAG46683 "TNNC1 [Homo sapiens]" 100.00 161 98.76 100.00 1.29e-106 GB AAA36772 "slow twitch skeletal/cardiac muscle troponin C [Homo sapiens]" 100.00 161 98.76 100.00 1.29e-106 GB AAA37492 "slow/cardiac troponin C, partial [Mus musculus]" 100.00 161 98.14 100.00 4.17e-106 GB AAA37493 "slow/cardiac troponin C [Mus musculus]" 100.00 161 98.14 100.00 4.17e-106 GB AAA48654 "slow muscle troponin C [Gallus gallus]" 100.00 161 100.00 100.00 1.41e-107 GB AAA49502 "troponin C, partial [Coturnix coturnix]" 100.00 160 97.52 99.38 4.09e-104 PIR S07450 "troponin C - quail [Phasianidae gen. sp.]" 100.00 161 98.14 100.00 1.94e-106 PIR TPHUCC "troponin C, cardiac and slow skeletal muscle - human" 100.00 161 98.76 100.00 1.29e-106 PRF 1403394A "slow/cardiac troponin C [Phasianidae gen. sp.]" 100.00 161 98.14 100.00 1.94e-106 PRF 1510257A "troponin C" 100.00 161 99.38 100.00 3.64e-107 PRF 750650A "troponin c,cardiac" 100.00 161 99.38 100.00 3.64e-107 REF NP_001029277 "troponin C, slow skeletal and cardiac muscles [Rattus norvegicus]" 100.00 161 98.14 100.00 4.17e-106 REF NP_001029523 "troponin C, slow skeletal and cardiac muscles [Bos taurus]" 100.00 161 99.38 100.00 3.64e-107 REF NP_001123715 "troponin C, slow skeletal and cardiac muscles [Sus scrofa]" 100.00 161 99.38 100.00 3.64e-107 REF NP_001272501 "troponin C, slow skeletal and cardiac muscles [Capra hircus]" 100.00 161 99.38 100.00 3.64e-107 REF NP_001291793 "troponin C type 1 (slow) [Ailuropoda melanoleuca]" 100.00 161 97.52 99.38 1.48e-105 SP P02591 "RecName: Full=Troponin C, slow skeletal and cardiac muscles; Short=TN-C" 100.00 161 98.76 100.00 1.29e-106 SP P05936 "RecName: Full=Troponin C, slow skeletal and cardiac muscles; Short=TN-C" 100.00 161 98.14 100.00 1.94e-106 SP P09860 "RecName: Full=Troponin C, slow skeletal and cardiac muscles; Short=TN-C" 100.00 161 100.00 100.00 1.41e-107 SP P19123 "RecName: Full=Troponin C, slow skeletal and cardiac muscles; Short=TN-C" 100.00 161 98.14 100.00 4.17e-106 SP P63315 "RecName: Full=Troponin C, slow skeletal and cardiac muscles; Short=TN-C" 100.00 161 99.38 100.00 3.64e-107 TPG DAA16908 "TPA: troponin C, slow skeletal and cardiac muscles [Bos taurus]" 100.00 161 99.38 100.00 3.64e-107 stop_ save_ save_TnT(160-193) _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common TnT(160-193) _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 34 _Mol_residue_sequence ; LAKADQKRGKKQTAREMKKK ILAERRKPLNIDHL ; loop_ _Residue_seq_code _Residue_label 1 LEU 2 ALA 3 LYS 4 ALA 5 ASP 6 GLN 7 LYS 8 ARG 9 GLY 10 LYS 11 LYS 12 GLN 13 THR 14 ALA 15 ARG 16 GLU 17 MET 18 LYS 19 LYS 20 LYS 21 ILE 22 LEU 23 ALA 24 GLU 25 ARG 26 ARG 27 LYS 28 PRO 29 LEU 30 ASN 31 ILE 32 ASP 33 HIS 34 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-30 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value DBJ BAB92975 "troponin T fast skeletal muscle type [Bos taurus]" 100.00 271 97.06 100.00 2.55e-12 DBJ BAB92976 "troponin T fast skeletal muscle type [Bos taurus]" 100.00 271 97.06 100.00 2.52e-12 DBJ BAB92977 "troponin T fast skeletal muscle type [Bos taurus]" 100.00 266 97.06 100.00 2.49e-12 DBJ BAB92978 "troponin T fast skeletal muscle type [Bos taurus]" 100.00 266 97.06 100.00 2.52e-12 DBJ BAB92979 "troponin T fast skeletal muscle type [Bos taurus]" 100.00 260 97.06 100.00 2.63e-12 EMBL CAE45814 "hypothetical protein [Homo sapiens]" 100.00 256 100.00 100.00 1.53e-12 EMBL CAG46725 "TNNT3 [Homo sapiens]" 100.00 256 100.00 100.00 1.45e-12 GB AAA36777 "troponin T [Homo sapiens]" 100.00 258 100.00 100.00 1.17e-12 GB AAA96440 "troponin T class IIa beta-4 [Rattus norvegicus]" 100.00 243 100.00 100.00 1.23e-12 GB AAA96441 "troponin T class Ia alpha-1 [Rattus norvegicus]" 100.00 259 100.00 100.00 1.08e-12 GB AAA96442 "troponin T class Ib beta-2 [Rattus norvegicus]" 100.00 247 100.00 100.00 1.27e-12 GB AAA96443 "troponin T class Ic beta-2 [Rattus norvegicus]" 100.00 248 100.00 100.00 1.24e-12 PIR TPRBTS "troponin T, fast skeletal muscle - rabbit" 100.00 266 100.00 100.00 1.45e-12 PRF 1810398A troponin:ISOTYPE=T 100.00 267 100.00 100.00 1.41e-12 REF NP_001001441 "troponin T, fast skeletal muscle [Bos taurus]" 100.00 250 97.06 100.00 1.68e-12 REF NP_001001863 "troponin T, fast skeletal muscle [Sus scrofa]" 100.00 271 97.06 100.00 2.50e-12 REF NP_001036245 "troponin T, fast skeletal muscle isoform 3 [Homo sapiens]" 100.00 250 100.00 100.00 1.28e-12 REF NP_001036246 "troponin T, fast skeletal muscle isoform 2 [Homo sapiens]" 100.00 256 100.00 100.00 1.45e-12 REF NP_001036247 "troponin T, fast skeletal muscle isoform 4 [Homo sapiens]" 100.00 250 100.00 100.00 1.26e-12 SP P02641 "RecName: Full=Troponin T, fast skeletal muscle; Short=TnTf; AltName: Full=Fast skeletal muscle troponin T; Short=fTnT [Oryctola" 100.00 279 100.00 100.00 1.44e-12 SP P09739 "RecName: Full=Troponin T, fast skeletal muscle; Short=TnTf; AltName: Full=Fast skeletal muscle troponin T; Short=fTnT [Rattus n" 100.00 259 100.00 100.00 1.07e-12 SP P45378 "RecName: Full=Troponin T, fast skeletal muscle; Short=TnTf; AltName: Full=Beta-TnTF; AltName: Full=Fast skeletal muscle troponi" 100.00 269 100.00 100.00 1.27e-12 SP Q75NG9 "RecName: Full=Troponin T, fast skeletal muscle; Short=TnTf [Sus scrofa]" 100.00 271 97.06 100.00 2.50e-12 SP Q8MKI3 "RecName: Full=Troponin T, fast skeletal muscle; Short=TnTf [Bos taurus]" 100.00 271 97.06 100.00 2.52e-12 TPG DAA13525 "TPA: troponin T, fast skeletal muscle [Bos taurus]" 100.00 250 97.06 100.00 1.68e-12 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $TnC chicken 9031 Eukaryota Metazoa Gallus gallus $TnT(160-193) chicken 9031 Eukaryota Metazoa Gallus gallus stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $TnC 'chemical synthesis' . . . . . $TnT(160-193) 'chemical synthesis' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $TnC 0.6 mM . . [U-15N] $TnT(160-193) . mM 21 62 'natural abundance' 'potassium chloride' 100 mM . . 'natural abundance' imidazole 10 mM . . 'natural abundance' dithiothreitol 20 mM . . 'natural abundance' CaCl2 6 mM . . 'natural abundance' 'sodium azide' 0.03 % . . 'natural abundance' '2,2-dimethyl-2-silapentane-5-sulfonic acid' 0.2 mM . . 'natural abundance' H2O 90 % . . 'natural abundance' D2O 10 % . . 'natural abundance' stop_ save_ save_sample_2 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Isotopic_labeling $TnC 0.9 mM '[U-13C; U-15N]' $TnT(160-193) 33 mM 'natural abundance' 'potassium chloride' 100 mM 'natural abundance' imidazole 10 mM 'natural abundance' dithiothreitol 20 mM 'natural abundance' CaCl2 6 mM 'natural abundance' 'sodium azide' 0.03 % 'natural abundance' '2,2-dimethyl-2-silapentane-5-sulfonic acid' 0.2 mM 'natural abundance' H2O 90 % 'natural abundance' D2O 10 % 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_VNMR _Saveframe_category software _Name VNMR _Version 6.1B loop_ _Vendor _Address _Electronic_address Varian . . stop_ loop_ _Task processing stop_ _Details . save_ save_NMRPipe _Saveframe_category software _Name NMRPipe _Version . loop_ _Vendor _Address _Electronic_address 'Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax' . . stop_ loop_ _Task processing stop_ _Details . save_ save_NMRView _Saveframe_category software _Name NMRView _Version . loop_ _Vendor _Address _Electronic_address 'Johnson, One Moon Scientific' . . stop_ loop_ _Task processing stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Varian _Model Unity _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_1D_1H-15N_HSQC_1 _Saveframe_category NMR_applied_experiment _Experiment_name '1D 1H-15N HSQC' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ save_1D_1H-15N_HSQC_3 _Saveframe_category NMR_applied_experiment _Experiment_name '1D 1H-15N HSQC' _Sample_label $sample_2 save_ save_2D_1H-15N_HSQC_4 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_2 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 298 . K pH 6.9 . pH pressure 1 . atm stop_ save_