data_16459 ####################### # Entry information # ####################### save_entry_information _Saveframe_category entry_information _Entry_title ; Solution Structure of the Ran-binding Domain 2 of RanBP and its Interaction with the C Terminus of Ran ; _BMRB_accession_number 16459 _BMRB_flat_file_name bmr16459.str _Entry_type new _Submission_date 2009-08-19 _Accession_date 2009-08-19 _Entry_origination author _NMR_STAR_version 2.1.1 _Experimental_method NMR _Details . loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Geyer J. P. . 2 Doker Rolf D. . 3 Kremer Werner D. . 4 Zhao Xiaodong . . 5 Kuhlmann Jurgen . . 6 Kalbitzer Hans R. . stop_ loop_ _Saveframe_category_type _Saveframe_category_type_count binding_constants 1 stop_ loop_ _Revision_date _Revision_keyword _Revision_author _Revision_detail 2010-03-08 original author . stop_ loop_ _Related_BMRB_accession_number _Relationship 5159 . stop_ save_ ############################# # Citation for this entry # ############################# save_entry_citation _Saveframe_category entry_citation _Citation_full . _Citation_title 'Solution Structure of the Ran-binding Domain 2 of RanBP2 and its Interaction with the C Terminus of Ran' _Citation_status published _Citation_type journal _CAS_abstract_code . _MEDLINE_UI_code . _PubMed_ID 15826666 loop_ _Author_ordinal _Author_family_name _Author_given_name _Author_middle_initials _Author_family_title 1 Geyer J. P. . 2 Doker Rolf . . 3 Kremer Werner . . 4 Zhao Xiaodong . . 5 Kuhlmann Jurgen . . 6 Kalbitzer Hans R. . stop_ _Journal_abbreviation 'J. Mol. Biol.' _Journal_name_full 'Journal of Molecular Biology' _Journal_volume 348 _Journal_issue 1 _Journal_CSD . _Book_chapter_title . _Book_volume . _Book_series . _Book_ISBN . _Conference_state_province . _Conference_abstract_number . _Page_first 711 _Page_last 725 _Year 2005 _Details . save_ ################################## # Molecular system description # ################################## save_assembly _Saveframe_category molecular_system _Mol_system_name 'Ran BD2/Ran C complex' _Enzyme_commission_number . loop_ _Mol_system_component_name _Mol_label RanBD2(delta)N $RanBD2(delta)N 'Ran C' $peptide_2 stop_ _System_molecular_weight . _System_physical_state native _System_oligomer_state ? _System_paramagnetic no _System_thiol_state . _Database_query_date . _Details . save_ ######################## # Monomeric polymers # ######################## save_RanBD2(delta)N _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common RanBD2(delta)N _Molecular_mass . _Mol_thiol_state 'not reported' _Details . ############################## # Polymer residue sequence # ############################## _Residue_count 130 _Mol_residue_sequence ; GSGEEDEKVLYSQRVKLFRF DAEVSQWKERGLGNLKILKN EVNGKLRMLMRREQVLKVCA NHWITTTMNLKPLSGSDRAW MWLASDFSDGDAKLEQLAAK FKTPELAEEFKQKFEECQRL LLDIPLQTPK ; loop_ _Residue_seq_code _Residue_label 1 GLY 2 SER 3 GLY 4 GLU 5 GLU 6 ASP 7 GLU 8 LYS 9 VAL 10 LEU 11 TYR 12 SER 13 GLN 14 ARG 15 VAL 16 LYS 17 LEU 18 PHE 19 ARG 20 PHE 21 ASP 22 ALA 23 GLU 24 VAL 25 SER 26 GLN 27 TRP 28 LYS 29 GLU 30 ARG 31 GLY 32 LEU 33 GLY 34 ASN 35 LEU 36 LYS 37 ILE 38 LEU 39 LYS 40 ASN 41 GLU 42 VAL 43 ASN 44 GLY 45 LYS 46 LEU 47 ARG 48 MET 49 LEU 50 MET 51 ARG 52 ARG 53 GLU 54 GLN 55 VAL 56 LEU 57 LYS 58 VAL 59 CYS 60 ALA 61 ASN 62 HIS 63 TRP 64 ILE 65 THR 66 THR 67 THR 68 MET 69 ASN 70 LEU 71 LYS 72 PRO 73 LEU 74 SER 75 GLY 76 SER 77 ASP 78 ARG 79 ALA 80 TRP 81 MET 82 TRP 83 LEU 84 ALA 85 SER 86 ASP 87 PHE 88 SER 89 ASP 90 GLY 91 ASP 92 ALA 93 LYS 94 LEU 95 GLU 96 GLN 97 LEU 98 ALA 99 ALA 100 LYS 101 PHE 102 LYS 103 THR 104 PRO 105 GLU 106 LEU 107 ALA 108 GLU 109 GLU 110 PHE 111 LYS 112 GLN 113 LYS 114 PHE 115 GLU 116 GLU 117 CYS 118 GLN 119 ARG 120 LEU 121 LEU 122 LEU 123 ASP 124 ILE 125 PRO 126 LEU 127 GLN 128 THR 129 PRO 130 LYS stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-02-05 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value PDB 1XKE "Solution Structure Of The Second Ran-Binding Domain From Human Ranbp2" 100.00 130 100.00 100.00 7.47e-89 stop_ save_ save_peptide_2 _Saveframe_category monomeric_polymer _Mol_type polymer _Mol_polymer_class protein _Name_common peptide_2 _Molecular_mass . _Mol_thiol_state 'not present' _Details . _Residue_count 28 _Mol_residue_sequence ; MDPALAAQYEHDLEVAQTTA LPDEDDDL ; loop_ _Residue_seq_code _Residue_label 1 MET 2 ASP 3 PRO 4 ALA 5 LEU 6 ALA 7 ALA 8 GLN 9 TYR 10 GLU 11 HIS 12 ASP 13 LEU 14 GLU 15 VAL 16 ALA 17 GLN 18 THR 19 THR 20 ALA 21 LEU 22 PRO 23 ASP 24 GLU 25 ASP 26 ASP 27 ASP 28 LEU stop_ _Sequence_homology_query_date . _Sequence_homology_query_revised_last_date 2015-01-30 loop_ _Database_name _Database_accession_code _Database_entry_mol_name _Sequence_query_to_submitted_percentage _Sequence_subject_length _Sequence_identity _Sequence_positive _Sequence_homology_expectation_value BMRB 19852 entity 100.00 216 100.00 100.00 4.03e-09 BMRB 19854 entity 100.00 216 100.00 100.00 4.03e-09 PDB 1A2K "Gdpran-Ntf2 Complex" 100.00 216 100.00 100.00 4.03e-09 PDB 1BYU "Canine Gdp-Ran" 100.00 216 100.00 100.00 4.56e-09 PDB 1I2M "Ran-Rcc1-So4 Complex" 100.00 216 100.00 100.00 4.03e-09 PDB 1IBR "Complex Of Ran With Importin Beta" 100.00 216 100.00 100.00 4.03e-09 PDB 1K5D "Crystal Structure Of Ran-Gppnhp-Ranbp1-Rangap Complex" 100.00 216 100.00 100.00 4.03e-09 PDB 1K5G "Crystal Structure Of Ran-Gdp-Alfx-Ranbp1-Rangap Complex" 100.00 216 100.00 100.00 4.03e-09 PDB 1QBK "Structure Of The Karyopherin Beta2-ran Gppnhp Nuclear Transport Complex" 96.43 216 100.00 100.00 6.86e-09 PDB 1QG2 "Canine Gdp-Ran R76e Mutant" 100.00 216 100.00 100.00 4.46e-09 PDB 1QG4 "Canine Gdp-Ran F72y Mutant" 100.00 216 100.00 100.00 4.28e-09 PDB 1RRP "Structure Of The Ran-Gppnhp-Ranbd1 Complex" 82.14 204 100.00 100.00 9.07e-07 PDB 2MMC "Nucleotide-free Human Ran Gtpase" 100.00 216 100.00 100.00 4.03e-09 PDB 2MMG "Structural Characterization Of The Mengovirus Leader Protein Bound To Ran Gtpase By Nuclear Magnetic Resonance" 100.00 216 100.00 100.00 4.03e-09 PDB 3A6P "Crystal Structure Of Exportin-5:rangtp:pre-Mirna Complex" 100.00 216 100.00 100.00 4.03e-09 PDB 3CH5 "The Crystal Structure Of The Rangdp-Nup153znf2 Complex" 100.00 216 100.00 100.00 4.03e-09 PDB 3EA5 "Kap95p Binding Induces The Switch Loops Of Rangdp To Adopt The Gtp- Bound Conformation: Implications For Nuclear Import Complex" 100.00 216 100.00 100.00 8.35e-10 PDB 3GJ0 "Crystal Structure Of Human Rangdp" 100.00 221 100.00 100.00 4.56e-09 PDB 3GJ3 "Crystal Structure Of Human Rangdp-Nup153znf2 Complex" 100.00 221 100.00 100.00 4.56e-09 PDB 3GJ4 "Crystal Structure Of Human Rangdp-Nup153znf3 Complex" 100.00 221 100.00 100.00 4.52e-09 PDB 3GJ5 "Crystal Structure Of Human Rangdp-Nup153znf4 Complex" 100.00 221 100.00 100.00 4.52e-09 PDB 3GJ6 "Crystal Structure Of Human Rangdp-Nup153znf1 Complex" 100.00 221 100.00 100.00 4.52e-09 PDB 3GJ7 "Crystal Structure Of Human Rangdp-Nup153znf12 Complex" 100.00 221 100.00 100.00 4.52e-09 PDB 3GJ8 "Crystal Structure Of Human Rangdp-Nup153znf34 Complex" 100.00 221 100.00 100.00 4.52e-09 PDB 3GJX "Crystal Structure Of The Nuclear Export Complex Crm1- Snurportin1-Rangtp" 100.00 216 100.00 100.00 3.95e-09 PDB 3RAN "Canine Gdp-Ran Q69l Mutant" 100.00 216 100.00 100.00 3.95e-09 PDB 4C0Q "Transportin 3 In Complex With Ran(q69l)gtp" 100.00 215 100.00 100.00 4.07e-09 PDB 4GMX "Crystal Structure Of Kpt185 In Complex With Crm1-Ran-Ranbp1" 100.00 216 100.00 100.00 4.03e-09 PDB 4GPT "Crystal Structure Of Kpt251 In Complex With Crm1-ran-ranbp1" 100.00 216 100.00 100.00 4.03e-09 PDB 4HAT "Crystal Structure Of Crm1 Inhibitor Leptomycin B In Complex With Crm1- Ran-ranbp1" 100.00 216 100.00 100.00 4.03e-09 PDB 4HAU "Crystal Structure Of Crm1 Inhibitor Ratjadone A In Complex With Crm1- Ran-ranbp1" 100.00 216 100.00 100.00 4.03e-09 PDB 4HAV "Crystal Structure Of Crm1 Inhibitor Anguinomycin A In Complex With Crm1-ran-ranbp1" 100.00 216 100.00 100.00 4.03e-09 PDB 4HAW "Crystal Structure Of Crm1 Inhibitor Leptomycin B In Complex With Crm1(k548a)-ran-ranbp1" 100.00 216 100.00 100.00 4.03e-09 PDB 4HAX "Crystal Structure Of Crm1 Inhibitor Ratjadone A In Complex With Crm1(k579a)-ran-ranbp1" 100.00 216 100.00 100.00 4.03e-09 PDB 4HAY "Crystal Structure Of Crm1 Inhibitor Leptomycin B In Complex With Crm1(k548e,k579q)-ran-ranbp1" 100.00 216 100.00 100.00 4.03e-09 PDB 4HAZ "Crystal Structure Of Crm1 Inhibitor Leptomycin B In Complex With Crm1(r543s,k548e,k579q)-ran-ranbp1" 100.00 216 100.00 100.00 4.03e-09 PDB 4HB0 "Crystal Structure Of Crm1 Inhibitor Leptomycin B In Complex With Crm1(k541q,k542q,r543s,k545q,k548q,k579q)-ran-ranbp1" 100.00 216 100.00 100.00 4.03e-09 PDB 4HB2 "Crystal Structure Of Crm1-ran-ranbp1" 100.00 216 100.00 100.00 4.03e-09 PDB 4HB3 "Crystal Structure Of Crm1(t539s)-ran-ranbp1 With Weakly Bound Unmodeled Leptomycin B" 100.00 216 100.00 100.00 4.03e-09 PDB 4HB4 "Crystal Structure Of Crm1 Inhibitor Leptomycin B In Complex With Crm1(537dltvk541/glceq)-ran-ranbp1" 100.00 216 100.00 100.00 4.03e-09 PDB 4OL0 "Crystal Structure Of Transportin-sr2, A Karyopherin Involved In Human Disease, In Complex With Ran" 100.00 216 100.00 100.00 4.03e-09 DBJ BAB27034 "unnamed protein product [Mus musculus]" 100.00 216 100.00 100.00 4.03e-09 DBJ BAB27105 "unnamed protein product [Mus musculus]" 100.00 216 100.00 100.00 4.28e-09 DBJ BAB93486 "member RAS oncogene family [Homo sapiens]" 100.00 128 100.00 100.00 3.41e-09 DBJ BAC36040 "unnamed protein product [Mus musculus]" 100.00 216 100.00 100.00 4.03e-09 DBJ BAC40068 "unnamed protein product [Mus musculus]" 100.00 216 100.00 100.00 4.03e-09 EMBL CAA77980 "Ran [Canis lupus familiaris]" 100.00 216 100.00 100.00 4.03e-09 EMBL CAG29343 "RAN [Homo sapiens]" 100.00 216 100.00 100.00 4.03e-09 EMBL CAH92646 "hypothetical protein [Pongo abelii]" 100.00 128 100.00 100.00 3.41e-09 EMBL CAH93110 "hypothetical protein [Pongo abelii]" 100.00 216 100.00 100.00 4.03e-09 EMBL CAI29709 "hypothetical protein [Pongo abelii]" 100.00 216 100.00 100.00 4.03e-09 GB AAA36546 "ras-like protein [Homo sapiens]" 100.00 216 100.00 100.00 4.03e-09 GB AAA64247 "Ran [Mus musculus]" 100.00 216 100.00 100.00 4.03e-09 GB AAB20486 "Ran=25 kda ras-related protein [Homo sapiens]" 100.00 36 100.00 100.00 7.22e-09 GB AAB24940 "Ran/TC4 gene product nuclear GTP-binding protein [human, Peptide, 216 aa]" 100.00 216 100.00 100.00 4.03e-09 GB AAB26214 "epidermal growth factor inhibitor, EGFI=34 kda ras-like protein {internal fragments} [Oryctolagus cuniculus]" 53.57 37 100.00 100.00 1.76e+00 REF NP_001029877 "GTP-binding nuclear protein Ran [Bos taurus]" 100.00 216 100.00 100.00 4.03e-09 REF NP_001126549 "GTP-binding nuclear protein Ran isoform 2 [Pongo abelii]" 100.00 128 100.00 100.00 3.41e-09 REF NP_001129282 "GTP-binding nuclear protein Ran isoform 1 [Pongo abelii]" 100.00 216 100.00 100.00 4.03e-09 REF NP_001155348 "GTP-binding nuclear protein Ran [Ovis aries]" 100.00 216 100.00 100.00 4.03e-09 REF NP_001181913 "GTP-binding nuclear protein Ran [Canis lupus familiaris]" 100.00 216 100.00 100.00 4.03e-09 SP P62825 "RecName: Full=GTP-binding nuclear protein Ran; AltName: Full=GTPase Ran; AltName: Full=Ras-like protein TC4; AltName: Full=Ras-" 100.00 216 100.00 100.00 4.03e-09 SP P62826 "RecName: Full=GTP-binding nuclear protein Ran; AltName: Full=Androgen receptor-associated protein 24; AltName: Full=GTPase Ran;" 100.00 216 100.00 100.00 4.03e-09 SP P62827 "RecName: Full=GTP-binding nuclear protein Ran; AltName: Full=GTPase Ran; AltName: Full=Ras-like protein TC4; AltName: Full=Ras-" 100.00 216 100.00 100.00 4.03e-09 SP P62828 "RecName: Full=GTP-binding nuclear protein Ran; AltName: Full=GTPase Ran; AltName: Full=Ras-like protein TC4; AltName: Full=Ras-" 100.00 216 100.00 100.00 4.03e-09 SP Q3T054 "RecName: Full=GTP-binding nuclear protein Ran; AltName: Full=GTPase Ran; AltName: Full=Ras-related nuclear protein [Bos taurus]" 100.00 216 100.00 100.00 4.03e-09 TPG DAA20885 "TPA: RAN, member RAS oncogene family-like [Bos taurus]" 100.00 146 100.00 100.00 4.52e-09 stop_ save_ #################### # Natural source # #################### save_natural_source _Saveframe_category natural_source loop_ _Mol_label _Organism_name_common _NCBI_taxonomy_ID _Superkingdom _Kingdom _Genus _Species $RanBD2(delta)N Human 9606 Eukaryota Metazoa Homo sapiens stop_ save_ ######################### # Experimental source # ######################### save_experimental_source _Saveframe_category experimental_source loop_ _Mol_label _Production_method _Host_organism_name_common _Genus _Species _Strain _Vector_name $RanBD2(delta)N 'chemical synthesis' . . . . na $peptide_2 'obtained from a vendor' . . . . . stop_ save_ ##################################### # Sample contents and methodology # ##################################### ######################## # Sample description # ######################## save_sample_1 _Saveframe_category sample _Sample_type solution _Details . loop_ _Mol_label _Concentration_value _Concentration_value_units _Concentration_min_value _Concentration_max_value _Isotopic_labeling $RanBD2(delta)N 0.5 mM . . 'natural abundance' $peptide_2 . mM 0.5 2.5 'natural abundance' 'sodium sulfate' 150 mM . . 'natural abundance' DTE 10 mM . . 'natural abundance' EDTA 0.5 mM . . 'natural abundance' 'sodium azide' 1 mM . . 'natural abundance' DSS 0.1 mM . . 'natural abundance' 'potassium phosphate buffer' 10 mM . . 'natural abundance' D2O 8 % . . 'natural abundance' H2O 92 % . . 'natural abundance' stop_ save_ ############################ # Computer software used # ############################ save_xwinnmr _Saveframe_category software _Name xwinnmr _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task processing stop_ _Details . save_ save_Auremol _Saveframe_category software _Name Auremol _Version . loop_ _Vendor _Address _Electronic_address 'Bruker Biospin' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_TALOS _Saveframe_category software _Name TALOS _Version . loop_ _Vendor _Address _Electronic_address 'Cornilescu, Delaglio and Bax' . . stop_ loop_ _Task 'structure solution' stop_ _Details . save_ save_X-PLOR_NIH _Saveframe_category software _Name X-PLOR_NIH _Version 2.9.6 loop_ _Vendor _Address _Electronic_address 'Schwieters, Kuszewski, Tjandra and Clore' . . stop_ loop_ _Task refinement stop_ _Details . save_ save_Procheck _Saveframe_category software _Name Procheck _Version . loop_ _Vendor _Address _Electronic_address 'Laskowski, MacArthur, Smith, Jones, Hutchinson, Morris, Moss and Tho' . . stop_ loop_ _Task 'data analysis' stop_ _Details . save_ save_Molmol _Saveframe_category software _Name Molmol _Version . loop_ _Vendor _Address _Electronic_address 'Koradi, Billeter and Wuthrich' . . stop_ loop_ _Task visualization stop_ _Details . save_ ######################### # Experimental detail # ######################### ################################## # NMR Spectrometer definitions # ################################## save_spectrometer_1 _Saveframe_category NMR_spectrometer _Manufacturer Bruker _Model DRX _Field_strength 600 _Details . save_ ############################# # NMR applied experiments # ############################# save_2D_1H-15N_TROSY_1 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N TROSY' _Sample_label $sample_1 save_ save_2D_1H-15N_HSQC_2 _Saveframe_category NMR_applied_experiment _Experiment_name '2D 1H-15N HSQC' _Sample_label $sample_1 save_ ####################### # Sample conditions # ####################### save_sample_conditions_1 _Saveframe_category sample_conditions _Details . loop_ _Variable_type _Variable_value _Variable_value_error _Variable_value_units temperature 298 . K pH 6.5 . pH pressure 1 . atm stop_ save_